MD10G1044500.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014655
Physical Location & Seq
Forward (+)
5998678 .. 6003718
5041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1044500.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGGCTGCAAATAAGTCTTCACGTTTGGGAGTAGTTTTCCTTTTAGGCCTCCCCCTCCTAGTCCTTGCTTGCTTCTTTCCCTGCAGTAGCTGCCACTGTGATGTCAGCGTAAACAAAAACACTGGTGATCACGACGGTGAGGCGGGGGCAGCTAGTGGAGGGATCAAAGGCATGTTTGTGTTTGGAAGCTCTTTGGTCGACAATGGCAACAACAACTTCCTCCCGGACTCACTGGCCAAAGCTGATTACTTTCCGTACGGAATCGATTTTCCTATCGGGCCTTCTGGGAGATTTACGAACGGGAAGAATGTTATCGACCTTCTCGGCGACCATCTCAAGCTTCCTTCCTTCGTTCCAGCATTTTCTGACCCCAAAACTAAAGGAACCAAAATCCTTCATGGCGTCAACTTTGCTTCTGGTGCTTCTGGTATTCTTGATGATACTGGTTCTTTAGCGGGGCATGTGATAAATCTGAATCAGCAAATCAGAAACTTTGAGGAGGTAACGTTGCCAGAGTTGGAAGCGGAGCGAGGCCGGTGCACAACCTCTCAGTCACTTGACGACTACTTGTTTGTGGTGGGAACTGGCGGAAACGATTACTCATTCAACTACTTCCTGACCACCTCCACCCGCAACGTCACCCTTGAAACCTTCACTGCCAATCTCACTGCCTCTTTGTCCACACAACTAAAGAAGTTGCACAGTTTAGGAGCTCGAAAGTTTGTGTTGATGTCAATAAATCCATTAGGGTGTAGTCCTGTGGTGAGGATGGGCCGACCAACACATAATGGTTGCATGCAGAAAATGAACCGGGCAGCTCATCTCTTCAATACTCACTTGAAGTCGTTGGTTGATGGTATGAGGGCAGAGATGCCGGGCTCCGCTCTTGTATTTGTTAACTCCTACAAGATCATTAGAGACATTATAAAAAATCCCATCTCCAAAGGATTCAAGGATGCTAGCACTACTTGTTGTGAAGTGGCATCAATAAATGAAGGAGGGAATGGAATTTTGTGCAAAAAGGGAGGCCAAGTGTGCGGAAACAGAAGCAGTCTTGTGTTCTTTGATGGCTTGCATCCAACGGAAGCTGTGAACGTTCAAATAGCAACCAAGGCCTTCGCCTCTTCCCTTAAAACTGAAGTTTATCCCACCAATATTGAGCGAATGACCACACGCAATATGTAG

Protein Analysis

395

Amino Acids

42.28

Weight (kDa)

8.57

Isoelectric Point (pI)

27.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 59 - 369 5.9e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014655)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12420
malus_domestica MD10G1044500.v1.1
prunus_persica Prupe.7G048300_v2.0.a1 Prupe.8G051400_v2.0.a1 Prupe.8G051400_v2.0.a1
pyrus_communis pycom10g03120
rosa_chinensis RchiOBHm_Chr6g0272561
rosa_laevigata RLG00000013666
rosa_multiflora Rmu_co8481641.1_g000001 Rmu_sc0003080.1_g000014
rosa_roxburghii Rroxscaffold_7G00196120
rosa_rugosa Rorug06G0070100
rosa_samantha Rh6AG186400 Rh6BG189500 Rh6CG187300 Rh6DG179000
rosa_wichuraiana Rw6G016060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 926
AccBSI CCGCTC 1 cut(s) 884
AccI GTMKAC 1 cut(s) 198
AciI CCGC 7 cut(s) 143, 455, 524, 588, 631, 882, 1038
AclI AACGTT 2 cut(s) 506, 1095
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 1 cut(s) 1008
AcuI CTGAAG 1 cut(s) 1158
AcyI GRCGYC 1 cut(s) 402
AfaI GTAC 1 cut(s) 257
AgsI TTSAA 6 cut(s) 607, 647, 829, 841, 952, 1100
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 8 cut(s) 90, 152, 189, 242, 340, 713, 818, 1088
AluI AGCT 8 cut(s) 90, 152, 189, 242, 340, 713, 818, 1088
Alw21I GWGCWC 2 cut(s) 542, 715
Alw26I GTCTC 1 cut(s) 912
Alw44I GTGCAC 1 cut(s) 538
AlwI GGATC 1 cut(s) 170
AlwNI CAGNNNCTG 1 cut(s) 90
AoxI GGCC 7 cut(s) 46, 234, 278, 532, 772, 1027, 1113
ApaLI GTGCAC 1 cut(s) 538
ApeKI GCWGC 4 cut(s) 5, 90, 149, 815
ApoI RAATTY 1 cut(s) 1008
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AspS9I GGNCC 2 cut(s) 278, 772
AsuC2I CCSGG 3 cut(s) 224, 812, 876
AsuHPI GGTGA 4 cut(s) 137, 149, 631, 775
AsuNHI GCTAGC 1 cut(s) 959
BaeGI GKGCMC 1 cut(s) 542
BalI TGGCCA 1 cut(s) 236
BanII GRGCYC 2 cut(s) 715, 881
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 542, 715
BbvI GCAGC 3 cut(s) 77, 161, 827
BccI CCATC 5 cut(s) 339, 763, 848, 944, 1061
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 3 cut(s) 224, 812, 876
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 3 cut(s) 59, 153, 960
BfmI CTRYAG 1 cut(s) 82
BisI GCNGC 4 cut(s) 6, 91, 150, 816
BlsI GCNGC 4 cut(s) 7, 92, 151, 817
Bme1390I CCNGG 3 cut(s) 224, 812, 876
BmgT120I GGNCC 2 cut(s) 278, 772
BmiI GGNNCC 2 cut(s) 385, 880
BmrFI CCNGG 3 cut(s) 224, 812, 876
BmsI GCATC 4 cut(s) 861, 946, 992, 1084
BmtI GCTAGC 1 cut(s) 963
BpiI GAAGAC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 3 cut(s) 224, 812, 876
Bsa29I ATCGAT 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 402
BsaJI CCNNGG 1 cut(s) 1110
BsaXI ACNNNNNCTCC 4 cut(s) 491, 521, 1017, 1047
Bse118I RCCGGY 1 cut(s) 534
Bse1I ACTGG 4 cut(s) 127, 237, 448, 589
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 1110
BseGI GGATG 3 cut(s) 774, 961, 1075
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 4 cut(s) 127, 237, 448, 589
BseRI GAGGAG 1 cut(s) 512
BseSI GKGCMC 1 cut(s) 542
BseXI GCAGC 3 cut(s) 77, 161, 827
BshFI GGCC 7 cut(s) 48, 236, 280, 534, 774, 1029, 1115
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 542, 715
BsiSI CCGG 4 cut(s) 224, 535, 811, 875
BsiWI CGTACG 1 cut(s) 255
BsmAI GTCTC 1 cut(s) 912
BsnI GGCC 7 cut(s) 48, 236, 280, 534, 774, 1029, 1115
Bsp1286I GDGCHC 3 cut(s) 542, 715, 881
Bsp143I GATC 3 cut(s) 127, 162, 909
BspACI CCGC 7 cut(s) 143, 455, 524, 588, 631, 882, 1038
BspANI GGCC 7 cut(s) 48, 236, 280, 534, 774, 1029, 1115
BspCNI CTCAG 1 cut(s) 562
BspDI ATCGAT 1 cut(s) 264
BspLI GGNNCC 2 cut(s) 385, 880
BspMAI CTGCAG 1 cut(s) 86
BspOI GCTAGC 1 cut(s) 963
BspPI GGATC 1 cut(s) 170
BsrBI CCGCTC 1 cut(s) 884
BsrFI RCCGGY 1 cut(s) 534
BsrI ACTGG 4 cut(s) 127, 237, 448, 589
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 1 cut(s) 1110
BssMI GATC 3 cut(s) 127, 162, 909
BssNI GRCGYC 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 1110
Bst4CI ACNGT 3 cut(s) 98, 137, 704
Bst6I CTCTTC 2 cut(s) 830, 1129
BstACI GRCGYC 1 cut(s) 402
BstAPI GCANNNNNTGC 1 cut(s) 90
BstC8I GCNNGC 4 cut(s) 70, 797, 961, 1073
BstDEI CTNAG 1 cut(s) 549
BstF5I GGATG 3 cut(s) 774, 961, 1075
BstKTI GATC 3 cut(s) 130, 165, 912
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 3 cut(s) 127, 162, 909
BstMWI GCNNNNNNNGC 5 cut(s) 90, 149, 419, 1035, 1112
BstNSI RCATGY 3 cut(s) 175, 464, 799
BstSCI CCNGG 3 cut(s) 222, 810, 874
BstSFI CTRYAG 1 cut(s) 82
BstSLI GKGCMC 1 cut(s) 542
BstV1I GCAGC 3 cut(s) 77, 161, 827
BstV2I GAAGAC 1 cut(s) 9
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 7 cut(s) 48, 236, 280, 534, 774, 1029, 1115
BsuTUI ATCGAT 1 cut(s) 264
BtsCI GGATG 3 cut(s) 774, 961, 1075
BtsI GCAGTG 2 cut(s) 654, 666
BtsIMutI CAGTG 5 cut(s) 94, 120, 230, 654, 666
Cac8I GCNNGC 4 cut(s) 70, 797, 961, 1073
CaiI CAGNNNCTG 1 cut(s) 90
Cfr10I RCCGGY 1 cut(s) 534
Cfr13I GGNCC 2 cut(s) 278, 772
ClaI ATCGAT 1 cut(s) 264
CseI GACGC 1 cut(s) 391
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 1138, 1173
CviAII CATG 4 cut(s) 172, 398, 461, 796
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 1 cut(s) 549
DpnI GATC 3 cut(s) 129, 164, 911
DpnII GATC 3 cut(s) 127, 162, 909
EaeI YGGCCR 1 cut(s) 234
Eam1104I CTCTTC 2 cut(s) 830, 1129
EarI CTCTTC 2 cut(s) 830, 1129
EciI GGCGGA 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 713
Eco130I CCWWGG 1 cut(s) 1110
Eco147I AGGCCT 2 cut(s) 48, 1115
Eco24I GRGCYC 2 cut(s) 715, 881
Eco53kI GAGCTC 1 cut(s) 713
Eco57I CTGAAG 1 cut(s) 1158
EcoICRI GAGCTC 1 cut(s) 713
EcoT14I CCWWGG 1 cut(s) 1110
EcoT38I GRGCYC 2 cut(s) 715, 881
ErhI CCWWGG 1 cut(s) 1110
FaeI CATG 4 cut(s) 175, 401, 464, 799
FaiI YATR 8 cut(s) 173, 399, 462, 786, 797, 860, 926, 1181
FatI CATG 4 cut(s) 171, 397, 460, 795
FauI CCCGC 3 cut(s) 136, 448, 638
FbaI TGATCA 1 cut(s) 127
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 6, 91, 150, 816
FokI GGATG 3 cut(s) 781, 968, 1062
FriOI GRGCYC 2 cut(s) 715, 881
Fsp4HI GCNGC 4 cut(s) 6, 91, 150, 816
FspBI CTAG 3 cut(s) 59, 153, 960
GluI GCNGC 4 cut(s) 6, 91, 150, 816
HaeIII GGCC 7 cut(s) 48, 236, 280, 534, 774, 1029, 1115
HapII CCGG 4 cut(s) 224, 535, 811, 875
HgaI GACGC 1 cut(s) 391
Hin1I GRCGYC 1 cut(s) 402
Hin1II CATG 4 cut(s) 175, 401, 464, 799
HincII GTYRAC 3 cut(s) 199, 406, 898
HindII GTYRAC 3 cut(s) 199, 406, 898
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 4 cut(s) 227, 261, 475, 948
HpaI GTTAAC 1 cut(s) 898
HpaII CCGG 4 cut(s) 224, 535, 811, 875
HphI GGTGA 4 cut(s) 137, 149, 631, 775
Hpy166II GTNNAC 7 cut(s) 112, 199, 406, 540, 681, 898, 1093
Hpy188I TCNGA 3 cut(s) 367, 474, 488
Hpy188III TCNNGA 3 cut(s) 131, 434, 616
Hpy8I GTNNAC 7 cut(s) 112, 199, 406, 540, 681, 898, 1093
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 8 cut(s) 291, 329, 354, 358, 404, 661, 989, 1126
HpyCH4III ACNGT 3 cut(s) 98, 137, 704
HpyCH4IV ACGT 4 cut(s) 22, 506, 636, 1095
HpyCH4V TGCA 8 cut(s) 8, 84, 540, 700, 795, 799, 1017, 1075
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 149, 419, 1035, 1112
HpyF3I CTNAG 1 cut(s) 549
HpySE526I ACGT 4 cut(s) 22, 506, 636, 1095
Hsp92I GRCGYC 1 cut(s) 402
Hsp92II CATG 4 cut(s) 175, 401, 464, 799
Ksp22I TGATCA 1 cut(s) 127
KspAI GTTAAC 1 cut(s) 898
Kzo9I GATC 3 cut(s) 127, 162, 909
LmnI GCTCC 3 cut(s) 526, 710, 884
Lsp1109I GCAGC 3 cut(s) 77, 161, 827
LweI GCATC 4 cut(s) 861, 946, 992, 1084
MaeI CTAG 3 cut(s) 59, 153, 960
MaeII ACGT 4 cut(s) 22, 506, 636, 1095
MaeIII GTNAC 3 cut(s) 502, 552, 637
MalI GATC 3 cut(s) 129, 164, 911
MbiI CCGCTC 1 cut(s) 884
MboI GATC 3 cut(s) 127, 162, 909
MboII GAAGA 4 cut(s) 9, 316, 817, 1116
MhlI GDGCHC 3 cut(s) 542, 715, 881
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 1 cut(s) 1008
MluNI TGGCCA 1 cut(s) 236
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 498, 1103
Mox20I TGGCCA 1 cut(s) 236
MscI TGGCCA 1 cut(s) 236
MseI TTAA 2 cut(s) 897, 1131
MslI CAYNNNNRTG 4 cut(s) 99, 135, 176, 748
Msp20I TGGCCA 1 cut(s) 236
MspI CCGG 4 cut(s) 224, 535, 811, 875
MspR9I CCNGG 3 cut(s) 224, 812, 876
MwoI GCNNNNNNNGC 5 cut(s) 90, 149, 419, 1035, 1112
NciI CCSGG 3 cut(s) 224, 812, 876
NdeII GATC 3 cut(s) 127, 162, 909
NheI GCTAGC 1 cut(s) 959
NlaIII CATG 4 cut(s) 175, 401, 464, 799
NlaIV GGNNCC 2 cut(s) 385, 880
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 2 cut(s) 552, 637
NspI RCATGY 3 cut(s) 175, 464, 799
OliI CACNNNNGTG 1 cut(s) 135
PaeI GCATGC 1 cut(s) 799
PceI AGGCCT 2 cut(s) 48, 1115
PfeI GAWTC 3 cut(s) 261, 475, 948
Pfl23II CGTACG 1 cut(s) 255
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 4 cut(s) 7, 92, 151, 817
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PsiI TTATAA 1 cut(s) 926
Psp124BI GAGCTC 1 cut(s) 715
Psp1406I AACGTT 2 cut(s) 506, 1095
PspLI CGTACG 1 cut(s) 255
PspN4I GGNNCC 2 cut(s) 385, 880
PspPI GGNCC 2 cut(s) 278, 772
PstI CTGCAG 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 90
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 4 cut(s) 99, 135, 176, 748
SacI GAGCTC 1 cut(s) 715
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 2 cut(s) 897, 1131
SatI GCNGC 4 cut(s) 6, 91, 150, 816
Sau3AI GATC 3 cut(s) 127, 162, 909
Sau96I GGNCC 2 cut(s) 278, 772
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 224, 812, 876
SduI GDGCHC 3 cut(s) 542, 715, 881
SfaNI GCATC 4 cut(s) 861, 946, 992, 1084
SfcI CTRYAG 1 cut(s) 82
SmiMI CAYNNNNRTG 4 cut(s) 99, 135, 176, 748
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
SphI GCATGC 1 cut(s) 799
Sse9I AATT 1 cut(s) 1008
SseBI AGGCCT 2 cut(s) 48, 1115
SsiI CCGC 7 cut(s) 143, 455, 524, 588, 631, 882, 1038
SspI AATATT 1 cut(s) 1156
SspMI CTAG 3 cut(s) 59, 153, 960
SstI GAGCTC 1 cut(s) 715
StuI AGGCCT 2 cut(s) 48, 1115
StyD4I CCNGG 3 cut(s) 222, 810, 874
StyI CCWWGG 1 cut(s) 1110
TaaI ACNGT 3 cut(s) 98, 137, 704
TaiI ACGT 4 cut(s) 25, 509, 639, 1098
TaqI TCGA 4 cut(s) 198, 264, 315, 715
TasI AATT 1 cut(s) 1008
TfiI GAWTC 3 cut(s) 261, 475, 948
Tru1I TTAA 2 cut(s) 897, 1131
Tru9I TTAA 2 cut(s) 897, 1131
TscAI CASTG 5 cut(s) 101, 127, 237, 661, 673
TseFI GTSAC 2 cut(s) 552, 637
TseI GCWGC 4 cut(s) 5, 90, 149, 815
Tsp45I GTSAC 2 cut(s) 552, 637
TspDTI ATGAA 3 cut(s) 386, 821, 1008
TspGWI ACGGA 3 cut(s) 243, 273, 1097
TspRI CASTG 5 cut(s) 101, 127, 237, 661, 673
VneI GTGCAC 1 cut(s) 538
XapI RAATTY 1 cut(s) 1008
XceI RCATGY 3 cut(s) 175, 464, 799
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 3 cut(s) 59, 153, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.