RLG00000013666

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014655
Physical Location & Seq
Forward (+)
35402657 .. 35405888
3232 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013666

Sequence Viewer

Length: 1158 bp
ATGGCTGGATGCTCATCTCTGAGACTTTTCATCTTAGGTGTCTCCCTCCTCTCTTTCTCGTTCCAATGCTTCTGCCACTGTAATGTGAACAGAAAAGGGGAGACAAGAAGTGTTGGGATCAAAGGGATGTTTGTGTTTGGAAGCTCCCTGGTTGACAATGGGAACAATAACTTCCTACAAAACTCACTGGCCAGAGCTGATTACTTGCCATATGGAGTTGATTTTCCTCTTGGCCCTTCTGGGAGATTCACAAATGGGAAGAACGTTATCGATCTTCTTGGAGAGCAGCTTAAGATACCTTCCTTCATTCCAGTATTCACTGACCCCCAAACTAAAGGAAGCAAAATTGTTCATGGTGTCAACTTCGCTTCTGGTGCTTCTGGTATACTCGATGATACTGGCTCCTTAGCGGGACGTGTAATGAGTCTAAATGAGCAAATTAGAAACTTCGAGGAGGTAACTGTGCCAGAGTTAAAAACCCAATTAGGGTTGGGGTGCAGTACAAGCTTTGAATCATCACTTGATAACTACTTATTTGTGGTGGGAACTGGCGGAAACGATTACTCTTTCAACTACTTCCTGGCCAACCCCCATAATGTCAGTCTTCAAGTCTTCACCGCCAATCTCACATCATCTCTCTCCACAAAACTAAAGAAGTTGCACAGTTTGGGAGCAAGAAAATTTGTGGTGATGTCAATAAACCCACTTGGGTGCACTCCTATGGCTATGAACATGATGAAGCTGCCAACCGTGACGCGTAATCGTTGCGTACAAGGTATGAACCGTGCAGTTCACCTTTTCAATTCTCGGTTCAAGTCCATGGTTGATGCCATAAGGGCCGAAATGCCCGGTTCAGCACTTGTATTCGTCAACTCCTACAAGATTATCAGGGATATTATCAAGAGTCCCATCTCCAAAGGATTCAAAGATTCGAGCGGTACGTGTTGTGAAGTGGCATCAATAAGTGAAGGAGGAAATGGAATCTTATGCAAAAGGGGAGGGCAAGTGTGCCTGAACAGAAGCAGTCATGTCTTCTTTGATGGCTTGCATCCAACGGAAGCTGTGAACGTTCAAATAGCAACCAAGGCATTCGTCTCCACTCTCAAAACTGAAGTTTATCCAACTAATATCCAACAAATGACCCAAATCCAAATATAG

Protein Analysis

386

Amino Acids

41.81

Weight (kDa)

9.17

Isoelectric Point (pI)

24.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 360 6.9e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014655)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12420
malus_domestica MD10G1044500.v1.1
prunus_persica Prupe.7G048300_v2.0.a1 Prupe.8G051400_v2.0.a1 Prupe.8G051400_v2.0.a1
pyrus_communis pycom10g03120
rosa_chinensis RchiOBHm_Chr6g0272561
rosa_laevigata RLG00000013666
rosa_multiflora Rmu_co8481641.1_g000001 Rmu_sc0003080.1_g000014
rosa_roxburghii Rroxscaffold_7G00196120
rosa_rugosa Rorug06G0070100
rosa_samantha Rh6AG186400 Rh6BG189500 Rh6CG187300 Rh6DG179000
rosa_wichuraiana Rw6G016060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 936
AccI GTMKAC 1 cut(s) 385
AccII CGCG 1 cut(s) 757
AciI CCGC 4 cut(s) 410, 552, 618, 936
AclI AACGTT 2 cut(s) 264, 1068
AclWI GGATC 1 cut(s) 125
AcoI YGGCCR 2 cut(s) 189, 582
AcsI RAATTY 1 cut(s) 680
AcuI CTGAAG 1 cut(s) 1131
AfaI GTAC 3 cut(s) 502, 771, 940
AfiI CCNNNNNNNGG 1 cut(s) 486
AflII CTTAAG 1 cut(s) 290
AflIII ACRYGT 3 cut(s) 415, 755, 941
AgsI TTSAA 7 cut(s) 512, 571, 608, 802, 814, 925, 1073
AjiI CACGTC 1 cut(s) 416
AjnI CCWGG 2 cut(s) 147, 579
AleI CACNNNNGTG 1 cut(s) 709
AluBI AGCT 6 cut(s) 144, 197, 289, 507, 742, 1061
AluI AGCT 6 cut(s) 144, 197, 289, 507, 742, 1061
Alw21I GWGCWC 1 cut(s) 716
Alw26I GTCTC 4 cut(s) 16, 46, 95, 1099
Alw44I GTGCAC 1 cut(s) 712
AlwI GGATC 1 cut(s) 125
AoxI GGCC 4 cut(s) 189, 232, 582, 837
ApaLI GTGCAC 1 cut(s) 712
ApeKI GCWGC 2 cut(s) 286, 742
ApoI RAATTY 1 cut(s) 680
AspS9I GGNCC 2 cut(s) 233, 837
AsuC2I CCSGG 1 cut(s) 849
AsuHPI GGTGA 3 cut(s) 607, 700, 785
BaeGI GKGCMC 1 cut(s) 716
BalI TGGCCA 2 cut(s) 191, 584
BbsI GAAGAC 3 cut(s) 596, 604, 1024
Bbv12I GWGCWC 1 cut(s) 716
BbvI GCAGC 2 cut(s) 298, 729
BccI CCATC 2 cut(s) 917, 1034
BciT130I CCWGG 2 cut(s) 149, 581
BcnI CCSGG 1 cut(s) 849
BcoDI GTCTC 4 cut(s) 16, 46, 95, 1099
BfrI CTTAAG 1 cut(s) 290
BglI GCCNNNNNGGC 1 cut(s) 836
BisI GCNGC 2 cut(s) 287, 743
BlsI GCNGC 2 cut(s) 288, 744
Bme1390I CCNGG 3 cut(s) 149, 581, 849
BmgBI CACGTC 1 cut(s) 416
BmgT120I GGNCC 2 cut(s) 233, 837
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 3 cut(s) 149, 581, 849
BmsI GCATC 3 cut(s) 817, 965, 1057
BpiI GAAGAC 3 cut(s) 596, 604, 1024
Bpu10I CCTNAGC 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 849
Bsa29I ATCGAT 1 cut(s) 270
BsaAI YACGTR 1 cut(s) 942
BsaBI GATNNNNATC 1 cut(s) 13
BsaJI CCNNGG 3 cut(s) 147, 819, 1083
BsaXI ACNNNNNCTCC 2 cut(s) 990, 1020
Bsc4I CCNNNNNNNGG 1 cut(s) 486
Bse1I ACTGG 4 cut(s) 192, 311, 403, 553
Bse8I GATNNNNATC 1 cut(s) 13
BseBI CCWGG 2 cut(s) 149, 581
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 3 cut(s) 147, 819, 1083
BseGI GGATG 3 cut(s) 14, 132, 1048
BseJI GATNNNNATC 1 cut(s) 13
BseLI CCNNNNNNNGG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 11
BseNI ACTGG 4 cut(s) 192, 311, 403, 553
BseRI GAGGAG 2 cut(s) 38, 467
BseSI GKGCMC 1 cut(s) 716
BseXI GCAGC 2 cut(s) 298, 729
BsgI GTGCAG 2 cut(s) 517, 807
Bsh1236I CGCG 1 cut(s) 757
BshFI GGCC 4 cut(s) 191, 234, 584, 839
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 716
BsiSI CCGG 1 cut(s) 849
BslFI GGGAC 2 cut(s) 426, 891
BslI CCNNNNNNNGG 1 cut(s) 486
BsmAI GTCTC 4 cut(s) 16, 46, 95, 1099
BsmBI CGTCTC 1 cut(s) 1099
BsmFI GGGAC 2 cut(s) 426, 891
BsmI GAATGC 1 cut(s) 1088
BsnI GGCC 4 cut(s) 191, 234, 584, 839
Bsp1286I GDGCHC 1 cut(s) 716
Bsp143I GATC 2 cut(s) 117, 271
Bsp19I CCATGG 1 cut(s) 819
BspACI CCGC 4 cut(s) 410, 552, 618, 936
BspANI GGCC 4 cut(s) 191, 234, 584, 839
BspCNI CTCAG 1 cut(s) 12
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 1 cut(s) 757
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 1 cut(s) 125
BspTI CTTAAG 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 936
BsrI ACTGG 4 cut(s) 192, 311, 403, 553
BssECI CCNNGG 3 cut(s) 147, 819, 1083
BssMI GATC 2 cut(s) 117, 271
BssNAI GTATAC 1 cut(s) 386
BssT1I CCWWGG 2 cut(s) 819, 1083
Bst1107I GTATAC 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 149, 581
Bst4CI ACNGT 5 cut(s) 80, 463, 665, 751, 785
BstAFI CTTAAG 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 942
BstC8I GCNNGC 1 cut(s) 1046
BstDEI CTNAG 3 cut(s) 20, 34, 406
BstDSI CCRYGG 1 cut(s) 819
BstF5I GGATG 3 cut(s) 14, 132, 1048
BstFNI CGCG 1 cut(s) 757
BstKTI GATC 2 cut(s) 120, 274
BstMAI GTCTC 4 cut(s) 16, 46, 95, 1099
BstMBI GATC 2 cut(s) 117, 271
BstMWI GCNNNNNNNGC 4 cut(s) 374, 504, 836, 1085
BstNI CCWGG 2 cut(s) 149, 581
BstSCI CCNGG 3 cut(s) 147, 579, 847
BstSLI GKGCMC 1 cut(s) 716
BstUI CGCG 1 cut(s) 757
BstV1I GCAGC 2 cut(s) 298, 729
BstV2I GAAGAC 3 cut(s) 596, 604, 1024
BstZ17I GTATAC 1 cut(s) 386
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 4 cut(s) 191, 234, 584, 839
BsuTUI ATCGAT 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 819
BtrI CACGTC 1 cut(s) 416
BtsCI GGATG 3 cut(s) 14, 132, 1048
BtsIMutI CAGTG 3 cut(s) 76, 185, 318
Cac8I GCNNGC 1 cut(s) 1046
Cfr13I GGNCC 2 cut(s) 233, 837
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 1 cut(s) 763
Csp6I GTAC 3 cut(s) 501, 770, 939
CviAII CATG 4 cut(s) 353, 733, 820, 1028
CviQI GTAC 3 cut(s) 501, 770, 939
DdeI CTNAG 3 cut(s) 20, 34, 406
DpnI GATC 2 cut(s) 119, 273
DpnII GATC 2 cut(s) 117, 271
EaeI YGGCCR 2 cut(s) 189, 582
EciI GGCGGA 1 cut(s) 567
Eco130I CCWWGG 2 cut(s) 819, 1083
Eco57I CTGAAG 1 cut(s) 1131
EcoRII CCWGG 2 cut(s) 147, 579
EcoT14I CCWWGG 2 cut(s) 819, 1083
ErhI CCWWGG 2 cut(s) 819, 1083
Esp3I CGTCTC 1 cut(s) 1099
FaeI CATG 4 cut(s) 356, 736, 823, 1031
FaqI GGGAC 2 cut(s) 426, 891
FatI CATG 4 cut(s) 352, 732, 819, 1027
FauI CCCGC 1 cut(s) 403
FauNDI CATATG 1 cut(s) 211
FblI GTMKAC 1 cut(s) 385
Fnu4HI GCNGC 2 cut(s) 287, 743
FokI GGATG 3 cut(s) 21, 139, 1035
Fsp4HI GCNGC 2 cut(s) 287, 743
GluI GCNGC 2 cut(s) 287, 743
HaeIII GGCC 4 cut(s) 191, 234, 584, 839
HapII CCGG 1 cut(s) 849
HgaI GACGC 1 cut(s) 763
Hin1II CATG 4 cut(s) 356, 736, 823, 1031
HincII GTYRAC 3 cut(s) 154, 361, 871
HindII GTYRAC 3 cut(s) 154, 361, 871
HindIII AAGCTT 1 cut(s) 505
HinfI GANTC 7 cut(s) 246, 424, 512, 904, 921, 929, 981
HpaII CCGG 1 cut(s) 849
HphI GGTGA 3 cut(s) 607, 700, 785
Hpy166II GTNNAC 8 cut(s) 88, 154, 361, 386, 714, 793, 871, 1066
Hpy188I TCNGA 1 cut(s) 21
Hpy188III TCNNGA 1 cut(s) 901
Hpy8I GTNNAC 8 cut(s) 88, 154, 361, 386, 714, 793, 871, 1066
HpyAV CCTTC 4 cut(s) 246, 309, 313, 962
HpyCH4III ACNGT 5 cut(s) 80, 463, 665, 751, 785
HpyCH4IV ACGT 4 cut(s) 264, 415, 941, 1068
HpyCH4V TGCA 6 cut(s) 498, 661, 714, 788, 990, 1048
HpyF10VI GCNNNNNNNGC 4 cut(s) 374, 504, 836, 1085
HpyF3I CTNAG 3 cut(s) 20, 34, 406
HpySE526I ACGT 4 cut(s) 264, 415, 941, 1068
Hsp92II CATG 4 cut(s) 356, 736, 823, 1031
Kzo9I GATC 2 cut(s) 117, 271
LmnI GCTCC 3 cut(s) 149, 407, 671
Lsp1109I GCAGC 2 cut(s) 298, 729
LweI GCATC 3 cut(s) 817, 965, 1057
MaeII ACGT 4 cut(s) 264, 415, 941, 1068
MaeIII GTNAC 2 cut(s) 457, 751
MalI GATC 2 cut(s) 119, 273
MbiI CCGCTC 1 cut(s) 936
MboI GATC 2 cut(s) 117, 271
MboII GAAGA 5 cut(s) 266, 271, 596, 604, 1024
MhlI GDGCHC 1 cut(s) 716
MlsI TGGCCA 2 cut(s) 191, 584
MluCI AATT 5 cut(s) 345, 438, 482, 680, 802
MluI ACGCGT 1 cut(s) 755
MluNI TGGCCA 2 cut(s) 191, 584
MlyI GAGTC 2 cut(s) 433, 913
MmeI TCCRAC 3 cut(s) 1076, 1145, 1156
MnlI CCTC 7 cut(s) 56, 59, 237, 445, 448, 965, 992
Mox20I TGGCCA 2 cut(s) 191, 584
MscI TGGCCA 2 cut(s) 191, 584
MseI TTAA 2 cut(s) 291, 473
MslI CAYNNNNRTG 3 cut(s) 81, 709, 719
Msp20I TGGCCA 2 cut(s) 191, 584
MspCI CTTAAG 1 cut(s) 290
MspI CCGG 1 cut(s) 849
MspR9I CCNGG 3 cut(s) 149, 581, 849
Mva1269I GAATGC 1 cut(s) 1088
MvaI CCWGG 2 cut(s) 149, 581
MvnI CGCG 1 cut(s) 757
MwoI GCNNNNNNNGC 4 cut(s) 374, 504, 836, 1085
NciI CCSGG 1 cut(s) 849
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 211
NdeII GATC 2 cut(s) 117, 271
NlaIII CATG 4 cut(s) 356, 736, 823, 1031
NlaIV GGNNCC 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 751
OliI CACNNNNGTG 1 cut(s) 709
PcsI WCGNNNNNNNCGW 1 cut(s) 938
PctI GAATGC 1 cut(s) 1088
PfeI GAWTC 5 cut(s) 246, 512, 921, 929, 981
PkrI GCNGC 2 cut(s) 288, 744
PleI GAGTC 2 cut(s) 432, 912
PpsI GAGTC 2 cut(s) 432, 912
Ppu21I YACGTR 1 cut(s) 942
Psp1406I AACGTT 2 cut(s) 264, 1068
Psp6I CCWGG 2 cut(s) 147, 579
PspGI CCWGG 2 cut(s) 147, 579
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 2 cut(s) 233, 837
RsaI GTAC 3 cut(s) 502, 771, 940
RsaNI GTAC 3 cut(s) 501, 770, 939
RseI CAYNNNNRTG 3 cut(s) 81, 709, 719
SaqAI TTAA 2 cut(s) 291, 473
SatI GCNGC 2 cut(s) 287, 743
Sau3AI GATC 2 cut(s) 117, 271
Sau96I GGNCC 2 cut(s) 233, 837
SchI GAGTC 2 cut(s) 433, 913
ScrFI CCNGG 3 cut(s) 149, 581, 849
SduI GDGCHC 1 cut(s) 716
SfaNI GCATC 3 cut(s) 817, 965, 1057
SmiMI CAYNNNNRTG 3 cut(s) 81, 709, 719
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
Sse9I AATT 5 cut(s) 345, 438, 482, 680, 802
SsiI CCGC 4 cut(s) 410, 552, 618, 936
StyD4I CCNGG 3 cut(s) 147, 579, 847
StyI CCWWGG 2 cut(s) 819, 1083
TaaI ACNGT 5 cut(s) 80, 463, 665, 751, 785
TaiI ACGT 4 cut(s) 267, 418, 944, 1071
TaqI TCGA 4 cut(s) 270, 390, 450, 932
TasI AATT 5 cut(s) 345, 438, 482, 680, 802
TatI WGTACW 1 cut(s) 500
TfiI GAWTC 5 cut(s) 246, 512, 921, 929, 981
Tru1I TTAA 2 cut(s) 291, 473
Tru9I TTAA 2 cut(s) 291, 473
TscAI CASTG 3 cut(s) 83, 192, 325
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 2 cut(s) 286, 742
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 6 cut(s) 19, 295, 341, 743, 752, 794
TspGWI ACGGA 1 cut(s) 1070
TspRI CASTG 3 cut(s) 83, 192, 325
Vha464I CTTAAG 1 cut(s) 290
VneI GTGCAC 1 cut(s) 712
XapI RAATTY 1 cut(s) 680
XmiI GTMKAC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.