FvH4_2g13700

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014656
Physical Location & Seq
Reverse (-)
11965572 .. 11977138
11567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13700.t3

Sequence Viewer

Length: 1098 bp
ATGGTCGTTTTTACAGAAGTAGATAAGAGAGATATGCTGAGAGCCAAGAGCTTGACCAAAGTGGCGGTGCCGCCTTCTCTTATAGACCATCCTTCTCCGGCTAACCTCCAATCCACTCGCCTCGCTCTCCATGTCAGCCAGGACGGCTCTTCTTGCTGGGTCTACATCGCCTCCGGTTGCCAAATTTACAAGCTCCAGGTTGCGTTGGACGATTCTCTGGTCAGTGAAGGGAAAGATAGCCTTCTGATTCCTGTTCACAGGCAGGTTATGAACTCATTTATGGTTAATCGGTGCCCGCATCGTTCAGAAATACAGAGTATTGTGCTTGCTGAAACTGAGAGCACTGGCTATTCAATGTTGGGAACTGTGGATTCTTATGGTCACCTTATTGTAACTAAACTAGACACTGATGGTAAAGATGTAGAGAAGCTTAGTTATTCAGTACTGCCTCGAGATTGTGGCCTTGGAGAGAGTGGATGGGCTGGTCTTTGCTTCAATCCAATACAATTGTCCATGGCAGCTGTTGCACGTAGCTTCTGCAAAACCGTTGATGTTTATGACCAGGATATTCACATTAGGACATTATGTACACTCTTGTATCCAACATCACTGTGCTTCATGCAAAGTTCGCGTTATGGAAATGAAAGCTCTATACTAGCTGTTACAGAAGGTTGCCAGCTGAGTATATGGGACTTGAGAATGAAAGAAAAGGGCGGCTGTCTACAACGAATATGTGGATCACTTGGTGATGACTTCTACACTGTTTGCAGTTCCTCAACTGGGAACATTGCGGTCGGTGGAGCTGATCGTACTGTGACGATCTATGATCCTCGCAGATGGGCCACGCTATCAAGATGGGTGCACTGCTCGAAATATGAGATAACTGGACTTGCATTCTCTTCAGTTGACTCCAACCATATCTATATACAAGGGGTTGATTACGAGGTCTTCTGTGGAGACTGGAAAGAGAATAGCAAGGTGATTTCCTTTAGAGGAGATTCAAACTGGCTTGGTTTTAGCAAGTGCCAGAACAGGGATGTTGTCGGTGGATGGTGCGACTCGGGTAGCATCTTTGTAGCTGATGTTGTAGACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

366

Amino Acids

40.3

Weight (kDa)

5.5

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13700 FvH4_2g13700 FvH4_2g13700
malus_domestica MD05G1026600.v1.1
prunus_persica Prupe.8G031100_v2.0.a1
pyrus_communis pycom05g01840
rosa_chinensis RchiOBHm_Chr6g0275381
rosa_laevigata RLG00000013470
rosa_multiflora Rmu_sc0004143.1_g000003
rosa_roxburghii Rroxscaffold_7G00193370
rosa_rugosa Rorug06G0091200 Rorug06G0091200 Rorug06G0091200
rosa_samantha Rh6AG205100 Rh6BG207900 Rh6CG210300
rosa_wichuraiana Rw6G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 253
AccB1I GGYRCC 2 cut(s) 67, 291
AccI GTMKAC 3 cut(s) 162, 721, 1089
AccII CGCG 1 cut(s) 631
AciI CCGC 5 cut(s) 65, 71, 296, 714, 791
AclWI GGATC 2 cut(s) 745, 821
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 885
AdeI CACNNNGTG 1 cut(s) 746
AfaI GTAC 3 cut(s) 444, 589, 811
AfiI CCNNNNNNNGG 2 cut(s) 780, 1033
AgsI TTSAA 3 cut(s) 354, 496, 1002
AjnI CCWGG 3 cut(s) 138, 195, 561
Alw21I GWGCWC 2 cut(s) 344, 864
Alw26I GTCTC 1 cut(s) 951
Alw44I GTGCAC 1 cut(s) 860
AlwI GGATC 2 cut(s) 745, 821
Ama87I CYCGRG 2 cut(s) 450, 1060
AoxI GGCC 2 cut(s) 460, 840
ApaLI GTGCAC 1 cut(s) 860
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 1 cut(s) 183
AspS9I GGNCC 1 cut(s) 840
AsuHPI GGTGA 3 cut(s) 374, 758, 991
AvaI CYCGRG 2 cut(s) 450, 1060
BaeGI GKGCMC 2 cut(s) 296, 864
BaeI ACNNNNGTAYC 2 cut(s) 581, 614
BanI GGYRCC 2 cut(s) 67, 291
BbsI GAAGAC 1 cut(s) 940
Bbv12I GWGCWC 2 cut(s) 344, 864
BbvI GCAGC 1 cut(s) 530
BccI CCATC 6 cut(s) 96, 404, 471, 831, 849, 1044
BceAI ACGGC 1 cut(s) 160
BciT130I CCWGG 3 cut(s) 140, 197, 563
BciVI GTATCC 1 cut(s) 609
BcoDI GTCTC 1 cut(s) 951
BfaI CTAG 2 cut(s) 401, 656
BfuAI ACCTGC 1 cut(s) 253
BfuI GTATCC 1 cut(s) 609
BglI GCCNNNNNGGC 1 cut(s) 144
BisI GCNGC 3 cut(s) 71, 519, 715
BlsI GCNGC 3 cut(s) 72, 520, 716
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 3 cut(s) 140, 197, 563
BmeT110I CYCGRG 2 cut(s) 450, 1060
BmgT120I GGNCC 1 cut(s) 840
BmiI GGNNCC 2 cut(s) 69, 293
BmrFI CCNGG 3 cut(s) 140, 197, 563
BmrI ACTGGG 1 cut(s) 789
BmsI GCATC 2 cut(s) 307, 1077
BmuI ACTGGG 1 cut(s) 789
BpiI GAAGAC 1 cut(s) 940
BpmI CTGGAG 1 cut(s) 179
BpuEI CTTGAG 1 cut(s) 715
BsaAI YACGTR 1 cut(s) 530
BsaJI CCNNGG 2 cut(s) 463, 513
BsaWI WCCGGW 1 cut(s) 173
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 780, 1033
Bse1I ACTGG 5 cut(s) 349, 784, 889, 965, 1010
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 3 cut(s) 140, 197, 563
BseDI CCNNGG 2 cut(s) 463, 513
BseGI GGATG 4 cut(s) 88, 482, 1042, 1055
BseLI CCNNNNNNNGG 2 cut(s) 780, 1033
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 3 cut(s) 29, 327, 671
BseNI ACTGG 5 cut(s) 349, 784, 889, 965, 1010
BseRI GAGGAG 1 cut(s) 1008
BseSI GKGCMC 2 cut(s) 296, 864
BseXI GCAGC 1 cut(s) 530
BseYI CCCAGC 1 cut(s) 156
Bsh1236I CGCG 1 cut(s) 631
Bsh1285I CGRYCG 1 cut(s) 795
BshFI GGCC 2 cut(s) 462, 842
BshNI GGYRCC 2 cut(s) 67, 291
BsiEI CGRYCG 1 cut(s) 795
BsiHKAI GWGCWC 2 cut(s) 344, 864
BsiHKCI CYCGRG 2 cut(s) 450, 1060
BsiSI CCGG 2 cut(s) 98, 174
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 2 cut(s) 780, 1033
BsmAI GTCTC 1 cut(s) 951
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 893
BsnI GGCC 2 cut(s) 462, 842
BsoBI CYCGRG 2 cut(s) 450, 1060
Bsp1286I GDGCHC 3 cut(s) 296, 344, 864
Bsp1407I TGTACA 1 cut(s) 587
Bsp143I GATC 4 cut(s) 737, 805, 819, 826
Bsp19I CCATGG 1 cut(s) 513
BspACI CCGC 5 cut(s) 65, 71, 296, 714, 791
BspANI GGCC 2 cut(s) 462, 842
BspCNI CTCAG 3 cut(s) 30, 328, 672
BspFNI CGCG 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 69, 293
BspMI ACCTGC 1 cut(s) 253
BspPI GGATC 2 cut(s) 745, 821
BspQI GCTCTTC 1 cut(s) 154
BspT107I GGYRCC 2 cut(s) 67, 291
BsrDI GCAATG 1 cut(s) 786
BsrGI TGTACA 1 cut(s) 587
BsrI ACTGG 5 cut(s) 349, 784, 889, 965, 1010
BssECI CCNNGG 2 cut(s) 463, 513
BssMI GATC 4 cut(s) 737, 805, 819, 826
BssT1I CCWWGG 2 cut(s) 463, 513
Bst2UI CCWGG 3 cut(s) 140, 197, 563
Bst4CI ACNGT 5 cut(s) 367, 547, 612, 763, 814
Bst6I CTCTTC 2 cut(s) 154, 904
BstAPI GCANNNNNTGC 1 cut(s) 524
BstAUI TGTACA 1 cut(s) 587
BstBAI YACGTR 1 cut(s) 530
BstC8I GCNNGC 3 cut(s) 296, 327, 677
BstDEI CTNAG 4 cut(s) 38, 336, 431, 680
BstDSI CCRYGG 1 cut(s) 513
BstEII GGTNACC 1 cut(s) 380
BstF5I GGATG 4 cut(s) 88, 482, 1042, 1055
BstFNI CGCG 1 cut(s) 631
BstKTI GATC 4 cut(s) 740, 808, 822, 829
BstMAI GTCTC 1 cut(s) 951
BstMBI GATC 4 cut(s) 737, 805, 819, 826
BstMCI CGRYCG 1 cut(s) 795
BstMWI GCNNNNNNNGC 4 cut(s) 144, 153, 524, 628
BstNI CCWGG 3 cut(s) 140, 197, 563
BstPI GGTNACC 1 cut(s) 380
BstSCI CCNGG 3 cut(s) 138, 195, 561
BstSLI GKGCMC 2 cut(s) 296, 864
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 1 cut(s) 530
BstV2I GAAGAC 1 cut(s) 940
BsuI GTATCC 1 cut(s) 609
BsuRI GGCC 2 cut(s) 462, 842
BtgI CCRYGG 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 151
BtsCI GGATG 4 cut(s) 88, 482, 1042, 1055
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 6 cut(s) 229, 342, 405, 608, 759, 862
BveI ACCTGC 1 cut(s) 253
Cac8I GCNNGC 3 cut(s) 296, 327, 677
Cfr13I GGNCC 1 cut(s) 840
Csp6I GTAC 3 cut(s) 443, 588, 810
CviAII CATG 3 cut(s) 131, 514, 619
CviQI GTAC 3 cut(s) 443, 588, 810
DdeI CTNAG 4 cut(s) 38, 336, 431, 680
DpnI GATC 4 cut(s) 739, 807, 821, 828
DpnII GATC 4 cut(s) 737, 805, 819, 826
DraIII CACNNNGTG 1 cut(s) 746
Eam1104I CTCTTC 2 cut(s) 154, 904
EarI CTCTTC 2 cut(s) 154, 904
Eco130I CCWWGG 2 cut(s) 463, 513
Eco57I CTGAAG 1 cut(s) 885
Eco88I CYCGRG 2 cut(s) 450, 1060
Eco91I GGTNACC 1 cut(s) 380
EcoO65I GGTNACC 1 cut(s) 380
EcoRII CCWGG 3 cut(s) 138, 195, 561
EcoT14I CCWWGG 2 cut(s) 463, 513
ErhI CCWWGG 2 cut(s) 463, 513
FaeI CATG 3 cut(s) 134, 517, 622
FalI AAGNNNNNCTT 2 cut(s) 225, 257
FaqI GGGAC 1 cut(s) 704
FatI CATG 3 cut(s) 130, 513, 618
FauI CCCGC 1 cut(s) 303
FblI GTMKAC 3 cut(s) 162, 721, 1089
Fnu4HI GCNGC 3 cut(s) 71, 519, 715
FokI GGATG 4 cut(s) 75, 489, 1049, 1062
Fsp4HI GCNGC 3 cut(s) 71, 519, 715
FspBI CTAG 2 cut(s) 401, 656
GluI GCNGC 3 cut(s) 71, 519, 715
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 179
HaeIII GGCC 2 cut(s) 462, 842
HapII CCGG 2 cut(s) 98, 174
Hin1II CATG 3 cut(s) 134, 517, 622
HincII GTYRAC 1 cut(s) 907
HindII GTYRAC 1 cut(s) 907
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 6 cut(s) 212, 247, 371, 908, 998, 1058
HpaII CCGG 2 cut(s) 98, 174
HphI GGTGA 3 cut(s) 374, 758, 991
Hpy166II GTNNAC 7 cut(s) 163, 256, 590, 722, 862, 907, 1090
Hpy188I TCNGA 2 cut(s) 246, 307
Hpy188III TCNNGA 2 cut(s) 452, 852
Hpy8I GTNNAC 7 cut(s) 163, 256, 590, 722, 862, 907, 1090
HpyAV CCTTC 5 cut(s) 84, 102, 221, 251, 662
HpyCH4III ACNGT 5 cut(s) 367, 547, 612, 763, 814
HpyCH4IV ACGT 1 cut(s) 529
HpyCH4V TGCA 6 cut(s) 527, 540, 622, 768, 862, 893
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 153, 524, 628
HpyF3I CTNAG 4 cut(s) 38, 336, 431, 680
HpySE526I ACGT 1 cut(s) 529
Hsp92II CATG 3 cut(s) 134, 517, 622
Kzo9I GATC 4 cut(s) 737, 805, 819, 826
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 2 cut(s) 198, 800
Lsp1109I GCAGC 1 cut(s) 530
LweI GCATC 2 cut(s) 307, 1077
MaeI CTAG 2 cut(s) 401, 656
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 4 cut(s) 380, 391, 661, 814
MalI GATC 4 cut(s) 739, 807, 821, 828
MboI GATC 4 cut(s) 737, 805, 819, 826
MboII GAAGA 3 cut(s) 141, 891, 940
MfeI CAATTG 1 cut(s) 506
MhlI GDGCHC 3 cut(s) 296, 344, 864
MluCI AATT 2 cut(s) 183, 506
MlyI GAGTC 2 cut(s) 902, 1052
MmeI TCCRAC 3 cut(s) 186, 626, 936
MnlI CCTC 8 cut(s) 116, 131, 181, 459, 784, 840, 937, 986
MseI TTAA 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 610
MspA1I CMGCKG 2 cut(s) 521, 679
MspI CCGG 2 cut(s) 98, 174
MspR9I CCNGG 3 cut(s) 140, 197, 563
MunI CAATTG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 893
MvaI CCWGG 3 cut(s) 140, 197, 563
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 4 cut(s) 144, 153, 524, 628
NcoI CCATGG 1 cut(s) 513
NdeII GATC 4 cut(s) 737, 805, 819, 826
NlaIII CATG 3 cut(s) 134, 517, 622
NlaIV GGNNCC 2 cut(s) 69, 293
NmuCI GTSAC 2 cut(s) 380, 814
PaeR7I CTCGAG 1 cut(s) 450
PciSI GCTCTTC 1 cut(s) 154
PctI GAATGC 1 cut(s) 893
PfeI GAWTC 4 cut(s) 212, 247, 371, 998
PkrI GCNGC 3 cut(s) 72, 520, 716
PleI GAGTC 2 cut(s) 902, 1052
PpsI GAGTC 2 cut(s) 902, 1052
Ppu21I YACGTR 1 cut(s) 530
Psp6I CCWGG 3 cut(s) 138, 195, 561
PspEI GGTNACC 1 cut(s) 380
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 3 cut(s) 138, 195, 561
PspN4I GGNNCC 2 cut(s) 69, 293
PspPI GGNCC 1 cut(s) 840
PvuII CAGCTG 2 cut(s) 521, 679
RsaI GTAC 3 cut(s) 444, 589, 811
RsaNI GTAC 3 cut(s) 443, 588, 810
RseI CAYNNNNRTG 1 cut(s) 610
SapI GCTCTTC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 3 cut(s) 71, 519, 715
Sau3AI GATC 4 cut(s) 737, 805, 819, 826
Sau96I GGNCC 1 cut(s) 840
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 2 cut(s) 902, 1052
ScrFI CCNGG 3 cut(s) 140, 197, 563
SduI GDGCHC 3 cut(s) 296, 344, 864
SfaNI GCATC 2 cut(s) 307, 1077
Sfr274I CTCGAG 1 cut(s) 450
SlaI CTCGAG 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 450, 694
SmoI CTYRAG 2 cut(s) 450, 694
Sse9I AATT 2 cut(s) 183, 506
SsiI CCGC 5 cut(s) 65, 71, 296, 714, 791
SspMI CTAG 2 cut(s) 401, 656
StyD4I CCNGG 3 cut(s) 138, 195, 561
StyI CCWWGG 2 cut(s) 463, 513
TaaI ACNGT 5 cut(s) 367, 547, 612, 763, 814
TaiI ACGT 1 cut(s) 532
TaqI TCGA 2 cut(s) 451, 869
TasI AATT 2 cut(s) 183, 506
TatI WGTACW 2 cut(s) 442, 587
TauI GCSGC 2 cut(s) 73, 717
TfiI GAWTC 4 cut(s) 212, 247, 371, 998
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 6 cut(s) 229, 349, 412, 615, 766, 869
TseFI GTSAC 2 cut(s) 380, 814
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 2 cut(s) 380, 814
TspDTI ATGAA 4 cut(s) 284, 607, 657, 716
TspRI CASTG 6 cut(s) 229, 349, 412, 615, 766, 869
VneI GTGCAC 1 cut(s) 860
XapI RAATTY 1 cut(s) 183
XhoI CTCGAG 1 cut(s) 450
XmiI GTMKAC 3 cut(s) 162, 721, 1089
XspI CTAG 2 cut(s) 401, 656
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.