Prupe.8G031100_v2.0.a1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0014656
Physical Location & Seq
Forward (+)
2946677 .. 2954834
8158 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G031100.1

Sequence Viewer

Length: 1110 bp
ATGTTGGAGGCCAAGAGTCTGAGCAAGGCGGTGGTGCCGTCCTCTCTCATAGAGAATCCTTCTCCTGGTAGCCTCCAGTCCACTCGCCTCGCTCTCCATGTTAGCGAGGACTGCTCTTCTTGTTGGGTCTACATCGCCTCCGGTTGCCAGATTTACAAACTCCAGATTCCATTGGAAGCTTCTCTGATCAGCGAAGGAAAAGAAAGCCTTCTGATCCCAGTACAAACTGAGGTTATGAACTCTTTGATGGTTAACCGCTGCCCTCATCGTTCAGAAATTCAGAGTATAGTTCTTGCTGAAACTGAGAGCACGGGCTATTCAATGTTGGGAACTGTTGATTCTTATGGTCATCTTATTGTATCTAAACTAGATACTACTGGTACAGATGTAGAGAGGCTTACTTATTCAGTATTGCCTCGTGATTGTGGCATTGGAGAAAGTGGTTGGGCGGGGCTTTGCTTCAGTCCAACACAATGGTCTACGGCAGCTGTTGCACGTAGCTTCTGCAAAACCATCGATGTTTATGACCAGGACATCCATGTCAGGACATTATGTACACTCTGGTACCCATCATCACTGAACTTCACAAAAAGTTCGCATTATGGGAACGAGGGCTCTATATTAGCAATCACAGAAGGTTCCCAGCTGACTATATGGGACTTGAGAATGAAAGAAAATGGTGGCTGTCTACAACGAATATGCGGTTCCCTTGGTGATGCGTTTTATGCTGTTTGCAGTTCTTCAACTGGGAATATTGCAGTCGGTGGAGCTGATCGTACAGTGACAATCTATGATCCTCGCAGATGGTCAGCATTATCAAGATGGGTGCACTGCTCAAAATATGAGATAACTGGGCTTGCATTCTCTTCAGTTGACTCCGACTATATCTACATACAAGGGGTTGACTATGAGGTCTTTTGTGGACAGTGGAAAGAAAGCAGCAAGGTATTTTCATTTAGAGGAGACTCAAACTGGCTTGGATTTAGCAAGTGCCAAAACAAGGATGTTCTAGGTGGATGGTGCGACTCGGGGAGTATCTTTGTGGCCGATGTTGTAGCGAAAGAAAGCGATACAAGCATGCTGCAAGGTTTTGCCAATGGATCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

370

Amino Acids

40.28

Weight (kDa)

5.05

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13700 FvH4_2g13700 FvH4_2g13700
malus_domestica MD05G1026600.v1.1
prunus_persica Prupe.8G031100_v2.0.a1
pyrus_communis pycom05g01840
rosa_chinensis RchiOBHm_Chr6g0275381
rosa_laevigata RLG00000013470
rosa_multiflora Rmu_sc0004143.1_g000003
rosa_roxburghii Rroxscaffold_7G00193370
rosa_rugosa Rorug06G0091200 Rorug06G0091200 Rorug06G0091200
rosa_samantha Rh6AG205100 Rh6BG207900 Rh6CG210300
rosa_wichuraiana Rw6G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 539, 911
Acc65I GGTACC 1 cut(s) 564
AccB1I GGYRCC 2 cut(s) 34, 564
AccI GTMKAC 3 cut(s) 129, 479, 688
AciI CCGC 4 cut(s) 29, 256, 449, 702
AclWI GGATC 3 cut(s) 208, 788, 1108
AcoI YGGCCR 1 cut(s) 1044
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 2 cut(s) 445, 852
AfaI GTAC 5 cut(s) 222, 382, 556, 566, 778
AfiI CCNNNNNNNGG 2 cut(s) 65, 1000
AgsI TTSAA 2 cut(s) 321, 744
AjnI CCWGG 2 cut(s) 64, 528
AluBI AGCT 5 cut(s) 179, 488, 501, 646, 770
AluI AGCT 5 cut(s) 179, 488, 501, 646, 770
Alw21I GWGCWC 2 cut(s) 311, 831
Alw26I GTCTC 1 cut(s) 957
Alw44I GTGCAC 1 cut(s) 827
AlwI GGATC 3 cut(s) 208, 788, 1108
Ama87I CYCGRG 1 cut(s) 1027
AoxI GGCC 2 cut(s) 9, 1044
ApaLI GTGCAC 1 cut(s) 827
ApeKI GCWGC 4 cut(s) 258, 485, 939, 1081
ApoI RAATTY 1 cut(s) 276
Asp700I GAANNNNTTC 1 cut(s) 207
Asp718I GGTACC 1 cut(s) 564
AsuHPI GGTGA 1 cut(s) 725
AvaI CYCGRG 1 cut(s) 1027
BaeGI GKGCMC 1 cut(s) 831
BaeI ACNNNNGTAYC 4 cut(s) 372, 405, 548, 581
BanI GGYRCC 2 cut(s) 34, 564
BanII GRGCYC 1 cut(s) 617
BauI CACGAG 1 cut(s) 417
Bbv12I GWGCWC 2 cut(s) 311, 831
BbvI GCAGC 4 cut(s) 245, 497, 951, 1068
BccI CCATC 6 cut(s) 241, 521, 577, 798, 816, 1011
BceAI ACGGC 2 cut(s) 22, 498
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BciT130I CCWGG 2 cut(s) 66, 530
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 957
BfaI CTAG 2 cut(s) 368, 1010
BisI GCNGC 4 cut(s) 259, 486, 940, 1082
BlsI GCNGC 4 cut(s) 260, 487, 941, 1083
Bme1390I CCNGG 2 cut(s) 66, 530
BmeT110I CYCGRG 1 cut(s) 1027
BmiI GGNNCC 4 cut(s) 36, 566, 640, 706
BmrFI CCNGG 2 cut(s) 66, 530
BmrI ACTGGG 3 cut(s) 212, 756, 861
BmsI GCATC 1 cut(s) 706
BmuI ACTGGG 3 cut(s) 212, 756, 861
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 2 cut(s) 59, 146
BpuEI CTTGAG 1 cut(s) 682
Bsa29I ATCGAT 1 cut(s) 516
BsaAI YACGTR 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 709
BsaWI WCCGGW 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 1000
Bse1I ACTGG 6 cut(s) 76, 218, 382, 751, 856, 977
BseBI CCWGG 2 cut(s) 66, 530
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 1 cut(s) 709
BseGI GGATG 3 cut(s) 534, 1009, 1022
BseLI CCNNNNNNNGG 2 cut(s) 65, 1000
BseMII CTCAG 3 cut(s) 11, 219, 294
BseNI ACTGG 6 cut(s) 76, 218, 382, 751, 856, 977
BseRI GAGGAG 1 cut(s) 975
BseSI GKGCMC 1 cut(s) 831
BseXI GCAGC 4 cut(s) 245, 497, 951, 1068
BseYI CCCAGC 1 cut(s) 642
BshFI GGCC 2 cut(s) 11, 1046
BshNI GGYRCC 2 cut(s) 34, 564
BshVI ATCGAT 1 cut(s) 516
BsiHKAI GWGCWC 2 cut(s) 311, 831
BsiHKCI CYCGRG 1 cut(s) 1027
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 2 cut(s) 65, 1000
BsmAI GTCTC 1 cut(s) 957
BsmFI GGGAC 1 cut(s) 671
BsmI GAATGC 1 cut(s) 860
BsnI GGCC 2 cut(s) 11, 1046
BsoBI CYCGRG 1 cut(s) 1027
Bsp1286I GDGCHC 3 cut(s) 311, 617, 831
Bsp1407I TGTACA 1 cut(s) 554
Bsp143I GATC 5 cut(s) 186, 213, 772, 793, 1100
BspACI CCGC 4 cut(s) 29, 256, 449, 702
BspANI GGCC 2 cut(s) 11, 1046
BspCNI CTCAG 3 cut(s) 12, 220, 295
BspDI ATCGAT 1 cut(s) 516
BspLI GGNNCC 4 cut(s) 36, 566, 640, 706
BspPI GGATC 3 cut(s) 208, 788, 1108
BspQI GCTCTTC 1 cut(s) 121
BspT107I GGYRCC 2 cut(s) 34, 564
BsrGI TGTACA 1 cut(s) 554
BsrI ACTGG 6 cut(s) 76, 218, 382, 751, 856, 977
BssECI CCNNGG 1 cut(s) 709
BssMI GATC 5 cut(s) 186, 213, 772, 793, 1100
BssSI CACGAG 1 cut(s) 417
BssT1I CCWWGG 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 417
Bst2UI CCWGG 2 cut(s) 66, 530
Bst4CI ACNGT 3 cut(s) 334, 781, 927
Bst6I CTCTTC 2 cut(s) 121, 871
BstAPI GCANNNNNTGC 1 cut(s) 491
BstAUI TGTACA 1 cut(s) 554
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 858, 1079
BstDEI CTNAG 3 cut(s) 20, 228, 303
BstF5I GGATG 3 cut(s) 534, 1009, 1022
BstKTI GATC 5 cut(s) 189, 216, 775, 796, 1103
BstMAI GTCTC 1 cut(s) 957
BstMBI GATC 5 cut(s) 186, 213, 772, 793, 1100
BstMWI GCNNNNNNNGC 4 cut(s) 111, 491, 725, 1074
BstNI CCWGG 2 cut(s) 66, 530
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 2 cut(s) 64, 528
BstSLI GKGCMC 1 cut(s) 831
BstV1I GCAGC 4 cut(s) 245, 497, 951, 1068
BstX2I RGATCY 1 cut(s) 1100
BstXI CCANNNNNNTGG 1 cut(s) 474
BstYI RGATCY 1 cut(s) 1100
Bsu15I ATCGAT 1 cut(s) 516
BsuRI GGCC 2 cut(s) 11, 1046
BsuTUI ATCGAT 1 cut(s) 516
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 3 cut(s) 534, 1009, 1022
BtsI GCAGTG 1 cut(s) 829
BtsIMutI CAGTG 4 cut(s) 575, 786, 829, 932
Cac8I GCNNGC 2 cut(s) 858, 1079
ClaI ATCGAT 1 cut(s) 516
Csp6I GTAC 5 cut(s) 221, 381, 555, 565, 777
CviAII CATG 3 cut(s) 98, 539, 1078
CviQI GTAC 5 cut(s) 221, 381, 555, 565, 777
DdeI CTNAG 3 cut(s) 20, 228, 303
DpnI GATC 5 cut(s) 188, 215, 774, 795, 1102
DpnII GATC 5 cut(s) 186, 213, 772, 793, 1100
DrdI GACNNNNNNGTC 2 cut(s) 539, 911
DseDI GACNNNNNNGTC 2 cut(s) 539, 911
EaeI YGGCCR 1 cut(s) 1044
Eam1104I CTCTTC 2 cut(s) 121, 871
EarI CTCTTC 2 cut(s) 121, 871
Eco130I CCWWGG 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 617
Eco57I CTGAAG 2 cut(s) 445, 852
Eco88I CYCGRG 1 cut(s) 1027
EcoRII CCWGG 2 cut(s) 64, 528
EcoT14I CCWWGG 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 709
FaeI CATG 3 cut(s) 101, 542, 1081
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FaqI GGGAC 1 cut(s) 671
FatI CATG 3 cut(s) 97, 538, 1077
FauI CCCGC 1 cut(s) 442
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 3 cut(s) 129, 479, 688
Fnu4HI GCNGC 4 cut(s) 259, 486, 940, 1082
FokI GGATG 3 cut(s) 521, 1016, 1029
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 4 cut(s) 259, 486, 940, 1082
FspBI CTAG 2 cut(s) 368, 1010
GluI GCNGC 4 cut(s) 259, 486, 940, 1082
GsaI CCCAGC 1 cut(s) 646
GsuI CTGGAG 2 cut(s) 59, 146
HaeIII GGCC 2 cut(s) 11, 1046
HapII CCGG 1 cut(s) 141
Hin1II CATG 3 cut(s) 101, 542, 1081
HincII GTYRAC 3 cut(s) 253, 874, 904
HindII GTYRAC 3 cut(s) 253, 874, 904
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 7 cut(s) 16, 55, 166, 338, 875, 965, 1025
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 1 cut(s) 141
HphI GGTGA 1 cut(s) 725
Hpy188I TCNGA 6 cut(s) 21, 186, 213, 274, 282, 880
Hpy188III TCNNGA 4 cut(s) 163, 419, 544, 819
HpyAV CCTTC 4 cut(s) 69, 188, 218, 629
HpyCH4III ACNGT 3 cut(s) 334, 781, 927
HpyCH4IV ACGT 1 cut(s) 496
HpyCH4V TGCA 7 cut(s) 494, 507, 735, 758, 829, 860, 1084
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 491, 725, 1074
HpyF3I CTNAG 3 cut(s) 20, 228, 303
HpySE526I ACGT 1 cut(s) 496
Hsp92II CATG 3 cut(s) 101, 542, 1081
KpnI GGTACC 1 cut(s) 568
Ksp22I TGATCA 1 cut(s) 186
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 5 cut(s) 186, 213, 772, 793, 1100
LguI GCTCTTC 1 cut(s) 121
LmnI GCTCC 1 cut(s) 767
Lsp1109I GCAGC 4 cut(s) 245, 497, 951, 1068
LweI GCATC 1 cut(s) 706
MaeI CTAG 2 cut(s) 368, 1010
MaeII ACGT 1 cut(s) 496
MaeIII GTNAC 1 cut(s) 781
MalI GATC 5 cut(s) 188, 215, 774, 795, 1102
MboI GATC 5 cut(s) 186, 213, 772, 793, 1100
MboII GAAGA 3 cut(s) 108, 732, 858
MflI RGATCY 1 cut(s) 1100
MhlI GDGCHC 3 cut(s) 311, 617, 831
MluCI AATT 1 cut(s) 276
MlyI GAGTC 4 cut(s) 25, 869, 959, 1019
MmeI TCCRAC 2 cut(s) 491, 903
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 1 cut(s) 252
MspA1I CMGCKG 3 cut(s) 258, 488, 646
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 2 cut(s) 66, 530
Mva1269I GAATGC 1 cut(s) 860
MvaI CCWGG 2 cut(s) 66, 530
MwoI GCNNNNNNNGC 4 cut(s) 111, 491, 725, 1074
NdeII GATC 5 cut(s) 186, 213, 772, 793, 1100
NlaIII CATG 3 cut(s) 101, 542, 1081
NlaIV GGNNCC 4 cut(s) 36, 566, 640, 706
NmuCI GTSAC 1 cut(s) 781
NspI RCATGY 1 cut(s) 1081
PaeI GCATGC 1 cut(s) 1081
PciSI GCTCTTC 1 cut(s) 121
PctI GAATGC 1 cut(s) 860
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 3 cut(s) 55, 166, 338
PkrI GCNGC 4 cut(s) 260, 487, 941, 1083
PleI GAGTC 4 cut(s) 24, 869, 959, 1019
PpsI GAGTC 4 cut(s) 24, 869, 959, 1019
Ppu21I YACGTR 1 cut(s) 497
Psp6I CCWGG 2 cut(s) 64, 528
PspFI CCCAGC 1 cut(s) 642
PspGI CCWGG 2 cut(s) 64, 528
PspN4I GGNNCC 4 cut(s) 36, 566, 640, 706
PsuI RGATCY 1 cut(s) 1100
PvuII CAGCTG 2 cut(s) 488, 646
RsaI GTAC 5 cut(s) 222, 382, 556, 566, 778
RsaNI GTAC 5 cut(s) 221, 381, 555, 565, 777
SapI GCTCTTC 1 cut(s) 121
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 4 cut(s) 259, 486, 940, 1082
Sau3AI GATC 5 cut(s) 186, 213, 772, 793, 1100
SchI GAGTC 4 cut(s) 25, 869, 959, 1019
ScrFI CCNGG 2 cut(s) 66, 530
SduI GDGCHC 3 cut(s) 311, 617, 831
SfaNI GCATC 1 cut(s) 706
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
SphI GCATGC 1 cut(s) 1081
Sse9I AATT 1 cut(s) 276
SsiI CCGC 4 cut(s) 29, 256, 449, 702
SspI AATATT 1 cut(s) 754
SspMI CTAG 2 cut(s) 368, 1010
StyD4I CCNGG 2 cut(s) 64, 528
StyI CCWWGG 1 cut(s) 709
TaaI ACNGT 3 cut(s) 334, 781, 927
TaiI ACGT 1 cut(s) 499
TaqI TCGA 1 cut(s) 516
TasI AATT 1 cut(s) 276
TatI WGTACW 2 cut(s) 220, 554
TfiI GAWTC 3 cut(s) 55, 166, 338
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 4 cut(s) 582, 786, 836, 932
TseFI GTSAC 1 cut(s) 781
TseI GCWGC 4 cut(s) 258, 485, 939, 1081
Tsp45I GTSAC 1 cut(s) 781
TspDTI ATGAA 3 cut(s) 251, 683, 942
TspRI CASTG 4 cut(s) 582, 786, 836, 932
VneI GTGCAC 1 cut(s) 827
XapI RAATTY 1 cut(s) 276
XceI RCATGY 1 cut(s) 1081
XmiI GTMKAC 3 cut(s) 129, 479, 688
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 2 cut(s) 368, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.