RLG00000013470

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014656
Physical Location & Seq
Forward (+)
32030562 .. 32044156
13595 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013470

Sequence Viewer

Length: 849 bp
ATGAACTCATCTATGGTTAATCGGTGTCCACATCGTTCAGAAATTCAGAGTATGGTGCTTGCTGAAACTGAGAGCACTGGCAATTCAATGTTGGGAACTGTGGATTCTTATGGTCACCTTATTGTATCTAAACTGGACACTAATGGTGAAGATGTAGAGAGGCTTACTTATTCAGTATTGCCTCGTGATTGCGGCATTGGAGAGAGTGGGTGGGCAGGTCTTTGCTTCAATCCAATACAATTGTCCACGGCAGCTGTTGCACATAGCTTCTGCAAAACCATTGATGTTTATGACCAGGATATCCACGTTAGGACATTATGTACACTCTTGTATCCAACATCACTGAGCTTCATGCAAAGTTCGCGTTATGGAAATGAAAGTTCTATATTAGCTGTCACTGAAGGTTGCCAGCTGAGTATATGGGACTTGAGAATGAAAGAAAATGGTGGCTGTCTACAACGAATATGTGGTTCCCTTGGTGATGTCTTCTACACTGTATGCAGTTCCTCAACTGGAAATATTGCAGTTGGTGGAGCTGATCGTACAGTGACGATATATGATCCTCGCAGATGGGCATCACTATCAAGATGGGTGAACTGCTCGAAATATGAGATAACTGGACTTGCTTTCTCTTCAATTGACTCCAACCATATCTATGTACAAGGGGTTGATTACGAGGTCTTCTGTGGAGACTGGAAAAGAAACAGCAAGTTATTTTCCTTTAGAGGAGATTCAAATTGGCTTGGTTTTAGCAAGTGCCAGAACAGTGATGTTGTCGGTGGATGGTGTGACTCGGGTAGCATCTTTGTGGCCAATGTTGTAGCTAAAGAGGGTGAAACAACCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

283

Amino Acids

30.97

Weight (kDa)

5.12

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 86 - 242 6.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 122 - 221 6.1e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13700 FvH4_2g13700 FvH4_2g13700
malus_domestica MD05G1026600.v1.1
prunus_persica Prupe.8G031100_v2.0.a1
pyrus_communis pycom05g01840
rosa_chinensis RchiOBHm_Chr6g0275381
rosa_laevigata RLG00000013470
rosa_multiflora Rmu_sc0004143.1_g000003
rosa_roxburghii Rroxscaffold_7G00193370
rosa_rugosa Rorug06G0091200 Rorug06G0091200 Rorug06G0091200
rosa_samantha Rh6AG205100 Rh6BG207900 Rh6CG210300
rosa_wichuraiana Rw6G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 206
AccI GTMKAC 1 cut(s) 454
AccII CGCG 1 cut(s) 364
AciI CCGC 1 cut(s) 192
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 3 cut(s) 322, 544, 660
AgsI TTSAA 4 cut(s) 87, 229, 636, 735
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 7 cut(s) 254, 267, 348, 392, 412, 536, 824
AluI AGCT 7 cut(s) 254, 267, 348, 392, 412, 536, 824
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 1 cut(s) 554
Ama87I CYCGRG 1 cut(s) 793
AoxI GGCC 1 cut(s) 810
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 42
AsuHPI GGTGA 5 cut(s) 107, 158, 491, 604, 845
AvaI CYCGRG 1 cut(s) 793
BaeI ACNNNNGTAYC 2 cut(s) 314, 347
BalI TGGCCA 1 cut(s) 812
BauI CACGAG 1 cut(s) 183
BbsI GAAGAC 2 cut(s) 478, 673
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 1 cut(s) 263
BccI CCATC 3 cut(s) 564, 582, 777
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 296
BciVI GTATCC 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 684
BfuAI ACCTGC 1 cut(s) 206
BfuI GTATCC 1 cut(s) 342
BisI GCNGC 2 cut(s) 193, 252
BlsI GCNGC 2 cut(s) 194, 253
Bme1390I CCNGG 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 793
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 2 cut(s) 584, 810
BpiI GAAGAC 2 cut(s) 478, 673
BpuEI CTTGAG 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 246, 475
Bse1I ACTGG 5 cut(s) 82, 138, 517, 622, 698
Bse8I GATNNNNATC 1 cut(s) 574
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 246, 475
BseGI GGATG 1 cut(s) 788
BseJI GATNNNNATC 1 cut(s) 574
BseMII CTCAG 3 cut(s) 60, 335, 404
BseNI ACTGG 5 cut(s) 82, 138, 517, 622, 698
BseRI GAGGAG 1 cut(s) 741
BseXI GCAGC 1 cut(s) 263
Bsh1236I CGCG 1 cut(s) 364
BshFI GGCC 1 cut(s) 812
BsiHKAI GWGCWC 1 cut(s) 77
BsiHKCI CYCGRG 1 cut(s) 793
BslFI GGGAC 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 684
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 1 cut(s) 812
BsoBI CYCGRG 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 77
Bsp1407I TGTACA 2 cut(s) 320, 658
Bsp143I GATC 2 cut(s) 538, 559
BspACI CCGC 1 cut(s) 192
BspANI GGCC 1 cut(s) 812
BspCNI CTCAG 3 cut(s) 61, 336, 405
BspFNI CGCG 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 472
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 554
BsrGI TGTACA 2 cut(s) 320, 658
BsrI ACTGG 5 cut(s) 82, 138, 517, 622, 698
BssECI CCNNGG 2 cut(s) 246, 475
BssMI GATC 2 cut(s) 538, 559
BssSI CACGAG 1 cut(s) 183
BssT1I CCWWGG 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 4 cut(s) 100, 496, 547, 767
Bst6I CTCTTC 1 cut(s) 637
BstAPI GCANNNNNTGC 1 cut(s) 257
BstAUI TGTACA 2 cut(s) 320, 658
BstC8I GCNNGC 2 cut(s) 60, 410
BstDEI CTNAG 3 cut(s) 69, 344, 413
BstDSI CCRYGG 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 113
BstF5I GGATG 1 cut(s) 788
BstFNI CGCG 1 cut(s) 364
BstKTI GATC 2 cut(s) 541, 562
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 2 cut(s) 538, 559
BstMWI GCNNNNNNNGC 2 cut(s) 257, 361
BstNI CCWGG 1 cut(s) 296
BstPI GGTNACC 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 294
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 1 cut(s) 263
BstV2I GAAGAC 2 cut(s) 478, 673
BsuI GTATCC 1 cut(s) 342
BsuRI GGCC 1 cut(s) 812
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 1 cut(s) 788
BtsIMutI CAGTG 6 cut(s) 75, 341, 396, 492, 552, 772
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 60, 410
Csp6I GTAC 3 cut(s) 321, 543, 659
CviAII CATG 2 cut(s) 352, 844
CviQI GTAC 3 cut(s) 321, 543, 659
DdeI CTNAG 3 cut(s) 69, 344, 413
DpnI GATC 2 cut(s) 540, 561
DpnII GATC 2 cut(s) 538, 559
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 637
EarI CTCTTC 1 cut(s) 637
Eco130I CCWWGG 1 cut(s) 475
Eco32I GATATC 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 420
Eco88I CYCGRG 1 cut(s) 793
Eco91I GGTNACC 1 cut(s) 113
EcoO65I GGTNACC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 294
EcoRV GATATC 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 475
FaeI CATG 2 cut(s) 355, 847
FaqI GGGAC 1 cut(s) 437
FatI CATG 2 cut(s) 351, 843
FblI GTMKAC 1 cut(s) 454
Fnu4HI GCNGC 2 cut(s) 193, 252
FokI GGATG 1 cut(s) 795
Fsp4HI GCNGC 2 cut(s) 193, 252
GluI GCNGC 2 cut(s) 193, 252
HaeIII GGCC 1 cut(s) 812
Hin1II CATG 2 cut(s) 355, 847
HinfI GANTC 4 cut(s) 104, 641, 731, 791
HphI GGTGA 5 cut(s) 107, 158, 491, 604, 845
Hpy166II GTNNAC 5 cut(s) 29, 246, 323, 455, 595
Hpy188I TCNGA 2 cut(s) 40, 48
Hpy188III TCNNGA 2 cut(s) 185, 585
Hpy8I GTNNAC 5 cut(s) 29, 246, 323, 455, 595
HpyAV CCTTC 1 cut(s) 395
HpyCH4III ACNGT 4 cut(s) 100, 496, 547, 767
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 5 cut(s) 260, 273, 355, 501, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 361
HpyF3I CTNAG 3 cut(s) 69, 344, 413
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 2 cut(s) 355, 847
Kzo9I GATC 2 cut(s) 538, 559
LmnI GCTCC 1 cut(s) 533
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 2 cut(s) 584, 810
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 4 cut(s) 113, 394, 547, 788
MalI GATC 2 cut(s) 540, 561
MboI GATC 2 cut(s) 538, 559
MboII GAAGA 4 cut(s) 161, 478, 624, 673
MfeI CAATTG 2 cut(s) 239, 636
MhlI GDGCHC 1 cut(s) 77
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 5 cut(s) 42, 82, 239, 636, 736
MluNI TGGCCA 1 cut(s) 812
MlyI GAGTC 2 cut(s) 635, 785
MmeI TCCRAC 2 cut(s) 359, 669
MnlI CCTC 7 cut(s) 153, 192, 517, 573, 670, 719, 823
Mox20I TGGCCA 1 cut(s) 812
MscI TGGCCA 1 cut(s) 812
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 2 cut(s) 654, 806
Msp20I TGGCCA 1 cut(s) 812
MspA1I CMGCKG 2 cut(s) 254, 412
MspR9I CCNGG 1 cut(s) 296
MunI CAATTG 2 cut(s) 239, 636
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 364
MwoI GCNNNNNNNGC 2 cut(s) 257, 361
NdeII GATC 2 cut(s) 538, 559
NlaIII CATG 2 cut(s) 355, 847
NlaIV GGNNCC 1 cut(s) 472
NmuCI GTSAC 4 cut(s) 113, 394, 547, 788
PfeI GAWTC 2 cut(s) 104, 731
PkrI GCNGC 2 cut(s) 194, 253
PleI GAGTC 2 cut(s) 635, 785
PpsI GAGTC 2 cut(s) 635, 785
Psp6I CCWGG 1 cut(s) 294
PspEI GGTNACC 1 cut(s) 113
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 472
PvuII CAGCTG 2 cut(s) 254, 412
RsaI GTAC 3 cut(s) 322, 544, 660
RsaNI GTAC 3 cut(s) 321, 543, 659
RseI CAYNNNNRTG 2 cut(s) 654, 806
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 2 cut(s) 193, 252
Sau3AI GATC 2 cut(s) 538, 559
SchI GAGTC 2 cut(s) 635, 785
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 1 cut(s) 77
SfaNI GCATC 2 cut(s) 584, 810
SmiMI CAYNNNNRTG 2 cut(s) 654, 806
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
Sse9I AATT 5 cut(s) 42, 82, 239, 636, 736
SsiI CCGC 1 cut(s) 192
SspI AATATT 1 cut(s) 520
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 4 cut(s) 100, 496, 547, 767
TaiI ACGT 1 cut(s) 309
TaqI TCGA 1 cut(s) 602
TasI AATT 5 cut(s) 42, 82, 239, 636, 736
TatI WGTACW 2 cut(s) 320, 658
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 2 cut(s) 104, 731
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 6 cut(s) 82, 348, 403, 499, 552, 772
TseFI GTSAC 4 cut(s) 113, 394, 547, 788
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 4 cut(s) 113, 394, 547, 788
TspDTI ATGAA 4 cut(s) 17, 340, 390, 449
TspRI CASTG 6 cut(s) 82, 348, 403, 499, 552, 772
XapI RAATTY 1 cut(s) 42
XmiI GTMKAC 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.