MD05G1026600.v1.1

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014656
Physical Location & Seq
Forward (+)
4236091 .. 4242617
6527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1026600.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGCTGGAGGCGAAGAGTCTGAGCAAGGCAGTAGTGCCGTCTTCCCTCATAGAGAATCCTTCTCCTGGTAGTCTCCAGTCCACTCGCCTGGCTCTCCATGTTGCCGAGGACTGCTCCTCGTGTTGGGTCTACATCGCCTCCGGCTGCCAAATTTATAAGCTTCAGATTCCATTGGAAGAGTCTCTGGTCGGTGAAGGGAAAGAAGGCCTTCTGATTCCTGTACTCAGGGAGGTTATGGATTCTTTTATGGTTAACCGCTGCCCTCATCGTTCTGAAATTCAGAGTATGGTGCTTGCTGAAACAGAGAGCAATGACTATTCAATGTTGGGAACTGTGGATTCTTATGGTCACTTTATTGTATCAAAACTAGATACTACTGGTGAAGAGCGACTTACTTATTCAGTATTGCCTCGTGATTGTGGCATTGGAGAAAGTGGTTGGGCAGGGCTTTCCTTCAGTCCTGCACAATGGTCTACGGCAGCCGTTGCGCGTAGCTTCTGCAAAACCATTGATGTTTATGACCAGGATATTCATGTCAGGACATTGTGTACACTTTGGTATCCGTCATCATTGAACTTCATAACATGTTCACATTATGGAAATGAGGGTTCTATATTAGCAATCACAGAAGGTTCCCAGCTGACTATATGGGACTTGAGAATGAAAGAAAATGGTGGCTGTCTACAACGAATATGTGGTTCCCTTGGTGATGCCTTTTATGCTGTGTGCTGTTCTTCAACTGGGAATGTTGCAGTCGGAGGAGCTGATCGTACAGTGACAATCTACGATCCTCGCAGATGGTCATCATTATCAAGATGGGTGCACTGCTCCAAATATGAGATAACTGGGCTTGCTTTCTCTTCAGTTGACTCCAACTACATCTACGTACAAGGGGTTGACTATGAGGTCTTATGTGGACAGTGGAAAGATAGCAGTAAGTTATTTTCATTTAGAGGAGACTCAAATTGGCTTGGTTTTAGCAAGTGCCAAAATAGGGATCTTCTAGGTGGATGGTGCGACTCTGGTAGTATCTTTGTGGCCGATGTTGTAGCTAAAGAGAAGGAAACAAACGCACCGACGTGTTCTTCCAGTGGATCTCCTGGCGCTCGTTCAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

373

Amino Acids

40.65

Weight (kDa)

5.08

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 157 - 318 1e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 198 - 303 6.3e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13700 FvH4_2g13700 FvH4_2g13700
malus_domestica MD05G1026600.v1.1
prunus_persica Prupe.8G031100_v2.0.a1
pyrus_communis pycom05g01840
rosa_chinensis RchiOBHm_Chr6g0275381
rosa_laevigata RLG00000013470
rosa_multiflora Rmu_sc0004143.1_g000003
rosa_roxburghii Rroxscaffold_7G00193370
rosa_rugosa Rorug06G0091200 Rorug06G0091200 Rorug06G0091200
rosa_samantha Rh6AG205100 Rh6BG207900 Rh6CG210300
rosa_wichuraiana Rw6G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
AasI GACNNNNNNGTC 1 cut(s) 905
AccI GTMKAC 3 cut(s) 129, 473, 682
AccII CGCG 1 cut(s) 490
AciI CCGC 1 cut(s) 256
AclWI GGATC 3 cut(s) 782, 1005, 1102
AcoI YGGCCR 1 cut(s) 1038
AcsI RAATTY 2 cut(s) 150, 276
AcuI CTGAAG 3 cut(s) 146, 439, 846
AfaI GTAC 4 cut(s) 222, 550, 772, 888
AfiI CCNNNNNNNGG 3 cut(s) 65, 123, 994
AflIII ACRYGT 2 cut(s) 584, 1079
AgsI TTSAA 4 cut(s) 321, 574, 738, 1113
AjiI CACGTC 1 cut(s) 1080
AjnI CCWGG 4 cut(s) 64, 87, 522, 1099
AleI CACNNNNGTG 1 cut(s) 1078
AluBI AGCT 5 cut(s) 160, 495, 640, 764, 1052
AluI AGCT 5 cut(s) 160, 495, 640, 764, 1052
Alw21I GWGCWC 1 cut(s) 825
Alw26I GTCTC 3 cut(s) 77, 186, 951
Alw44I GTGCAC 1 cut(s) 821
AlwI GGATC 3 cut(s) 782, 1005, 1102
AoxI GGCC 2 cut(s) 205, 1038
ApaLI GTGCAC 1 cut(s) 821
ApeKI GCWGC 3 cut(s) 144, 258, 479
ApoI RAATTY 2 cut(s) 150, 276
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 2 cut(s) 490, 1106
AsuHPI GGTGA 3 cut(s) 203, 392, 719
BaeGI GKGCMC 1 cut(s) 825
BaeI ACNNNNGTAYC 2 cut(s) 542, 575
BauI CACGAG 2 cut(s) 118, 411
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 825
BbvI GCAGC 3 cut(s) 131, 245, 491
BccI CCATC 3 cut(s) 792, 810, 1005
BceAI ACGGC 3 cut(s) 22, 467, 492
BciT130I CCWGG 4 cut(s) 66, 89, 524, 1101
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 3 cut(s) 77, 186, 951
BfaI CTAG 2 cut(s) 368, 1004
BfoI RGCGCY 1 cut(s) 1107
BfuI GTATCC 1 cut(s) 570
BisI GCNGC 3 cut(s) 145, 259, 480
BlsI GCNGC 3 cut(s) 146, 260, 481
Bme1390I CCNGG 4 cut(s) 66, 89, 524, 1101
BmgBI CACGTC 1 cut(s) 1080
BmiI GGNNCC 2 cut(s) 634, 700
BmrFI CCNGG 4 cut(s) 66, 89, 524, 1101
BmrI ACTGGG 2 cut(s) 750, 855
BmsI GCATC 1 cut(s) 700
BmuI ACTGGG 2 cut(s) 750, 855
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 2 cut(s) 26, 59
BpuEI CTTGAG 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 886
BsaBI GATNNNNATC 1 cut(s) 802
BsaJI CCNNGG 2 cut(s) 105, 703
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 123, 994
Bse1I ACTGG 5 cut(s) 76, 382, 745, 850, 1089
Bse3DI GCAATG 1 cut(s) 316
Bse8I GATNNNNATC 1 cut(s) 802
BseBI CCWGG 4 cut(s) 66, 89, 524, 1101
BseDI CCNNGG 2 cut(s) 105, 703
BseGI GGATG 1 cut(s) 1016
BseJI GATNNNNATC 1 cut(s) 802
BseLI CCNNNNNNNGG 3 cut(s) 65, 123, 994
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 11, 238
BseNI ACTGG 5 cut(s) 76, 382, 745, 850, 1089
BseRI GAGGAG 3 cut(s) 106, 774, 969
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 3 cut(s) 131, 245, 491
BseYI CCCAGC 1 cut(s) 636
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 490
BshFI GGCC 2 cut(s) 207, 1040
BsiHKAI GWGCWC 1 cut(s) 825
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 3 cut(s) 65, 123, 994
BsmAI GTCTC 3 cut(s) 77, 186, 951
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 2 cut(s) 207, 1040
Bsp1286I GDGCHC 1 cut(s) 825
Bsp1407I TGTACA 1 cut(s) 548
Bsp143I GATC 4 cut(s) 766, 787, 997, 1094
BspACI CCGC 1 cut(s) 256
BspANI GGCC 2 cut(s) 207, 1040
BspCNI CTCAG 2 cut(s) 12, 237
BspFNI CGCG 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 634, 700
BspPI GGATC 3 cut(s) 782, 1005, 1102
BspQI GCTCTTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 316
BsrGI TGTACA 1 cut(s) 548
BsrI ACTGG 5 cut(s) 76, 382, 745, 850, 1089
BssECI CCNNGG 2 cut(s) 105, 703
BssMI GATC 4 cut(s) 766, 787, 997, 1094
BssSI CACGAG 2 cut(s) 118, 411
BssT1I CCWWGG 1 cut(s) 703
Bst2BI CACGAG 2 cut(s) 118, 411
Bst2UI CCWGG 4 cut(s) 66, 89, 524, 1101
Bst4CI ACNGT 3 cut(s) 334, 775, 921
Bst6I CTCTTC 4 cut(s) 8, 171, 378, 865
BstAPI GCANNNNNTGC 1 cut(s) 485
BstAUI TGTACA 1 cut(s) 548
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 2 cut(s) 294, 852
BstDEI CTNAG 2 cut(s) 20, 224
BstF5I GGATG 1 cut(s) 1016
BstFNI CGCG 1 cut(s) 490
BstH2I RGCGCY 1 cut(s) 1107
BstHHI GCGC 2 cut(s) 490, 1106
BstKTI GATC 4 cut(s) 769, 790, 1000, 1097
BstMAI GTCTC 3 cut(s) 77, 186, 951
BstMBI GATC 4 cut(s) 766, 787, 997, 1094
BstMWI GCNNNNNNNGC 2 cut(s) 485, 719
BstNI CCWGG 4 cut(s) 66, 89, 524, 1101
BstNSI RCATGY 1 cut(s) 588
BstSCI CCNGG 4 cut(s) 64, 87, 522, 1099
BstSLI GKGCMC 1 cut(s) 825
BstSNI TACGTA 1 cut(s) 886
BstUI CGCG 1 cut(s) 490
BstV1I GCAGC 3 cut(s) 131, 245, 491
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 2 cut(s) 997, 1094
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 2 cut(s) 997, 1094
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 2 cut(s) 207, 1040
BtgZI GCGATG 1 cut(s) 118
BtrI CACGTC 1 cut(s) 1080
BtsCI GGATG 1 cut(s) 1016
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 4 cut(s) 780, 823, 926, 1096
Cac8I GCNNGC 2 cut(s) 294, 852
CfoI GCGC 2 cut(s) 490, 1106
Csp6I GTAC 4 cut(s) 221, 549, 771, 887
CviAII CATG 3 cut(s) 98, 533, 585
CviQI GTAC 4 cut(s) 221, 549, 771, 887
DdeI CTNAG 2 cut(s) 20, 224
DpnI GATC 4 cut(s) 768, 789, 999, 1096
DpnII GATC 4 cut(s) 766, 787, 997, 1094
DrdI GACNNNNNNGTC 1 cut(s) 905
DseDI GACNNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 1 cut(s) 1038
Eam1104I CTCTTC 4 cut(s) 8, 171, 378, 865
EarI CTCTTC 4 cut(s) 8, 171, 378, 865
Eco105I TACGTA 1 cut(s) 886
Eco130I CCWWGG 1 cut(s) 703
Eco147I AGGCCT 1 cut(s) 207
Eco57I CTGAAG 3 cut(s) 146, 439, 846
EcoRII CCWGG 4 cut(s) 64, 87, 522, 1099
EcoT14I CCWWGG 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 703
FaeI CATG 3 cut(s) 101, 536, 588
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 375, 407
FaqI GGGAC 1 cut(s) 665
FatI CATG 3 cut(s) 97, 532, 584
FblI GTMKAC 3 cut(s) 129, 473, 682
Fnu4HI GCNGC 3 cut(s) 145, 259, 480
FokI GGATG 1 cut(s) 1023
Fsp4HI GCNGC 3 cut(s) 145, 259, 480
FspBI CTAG 2 cut(s) 368, 1004
GlaI GCGC 2 cut(s) 489, 1105
GluI GCNGC 3 cut(s) 145, 259, 480
GsaI CCCAGC 1 cut(s) 640
GsuI CTGGAG 2 cut(s) 26, 59
HaeII RGCGCY 1 cut(s) 1107
HaeIII GGCC 2 cut(s) 207, 1040
HapII CCGG 1 cut(s) 141
HhaI GCGC 2 cut(s) 490, 1106
Hin1II CATG 3 cut(s) 101, 536, 588
Hin6I GCGC 2 cut(s) 488, 1104
HinP1I GCGC 2 cut(s) 488, 1104
HincII GTYRAC 3 cut(s) 253, 868, 898
HindII GTYRAC 3 cut(s) 253, 868, 898
HindIII AAGCTT 1 cut(s) 158
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 1 cut(s) 141
HphI GGTGA 3 cut(s) 203, 392, 719
Hpy188I TCNGA 6 cut(s) 21, 165, 213, 274, 282, 758
Hpy188III TCNNGA 3 cut(s) 413, 538, 813
Hpy99I CGWCG 1 cut(s) 1081
HpyAV CCTTC 7 cut(s) 69, 188, 197, 218, 463, 623, 1054
HpyCH4III ACNGT 3 cut(s) 334, 775, 921
HpyCH4IV ACGT 2 cut(s) 885, 1079
HpyCH4V TGCA 4 cut(s) 464, 501, 752, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 485, 719
HpyF3I CTNAG 2 cut(s) 20, 224
HpySE526I ACGT 2 cut(s) 885, 1079
Hsp92II CATG 3 cut(s) 101, 536, 588
HspAI GCGC 2 cut(s) 488, 1104
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 4 cut(s) 766, 787, 997, 1094
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 3 cut(s) 119, 761, 833
Lsp1109I GCAGC 3 cut(s) 131, 245, 491
LweI GCATC 1 cut(s) 700
MaeI CTAG 2 cut(s) 368, 1004
MaeII ACGT 2 cut(s) 885, 1079
MaeIII GTNAC 2 cut(s) 347, 775
MalI GATC 4 cut(s) 768, 789, 999, 1096
MboI GATC 4 cut(s) 766, 787, 997, 1094
MboII GAAGA 8 cut(s) 25, 33, 188, 395, 726, 852, 992, 1077
MflI RGATCY 2 cut(s) 997, 1094
MhlI GDGCHC 1 cut(s) 825
MluCI AATT 3 cut(s) 150, 276, 964
MlyI GAGTC 5 cut(s) 25, 188, 863, 953, 1013
MmeI TCCRAC 2 cut(s) 736, 897
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 2 cut(s) 252, 1117
MslI CAYNNNNRTG 1 cut(s) 1078
MspA1I CMGCKG 2 cut(s) 258, 640
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 4 cut(s) 66, 89, 524, 1101
MvaI CCWGG 4 cut(s) 66, 89, 524, 1101
MvnI CGCG 1 cut(s) 490
MwoI GCNNNNNNNGC 2 cut(s) 485, 719
NdeII GATC 4 cut(s) 766, 787, 997, 1094
NlaIII CATG 3 cut(s) 101, 536, 588
NlaIV GGNNCC 2 cut(s) 634, 700
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 347, 775
NspI RCATGY 1 cut(s) 588
OliI CACNNNNGTG 1 cut(s) 1078
PceI AGGCCT 1 cut(s) 207
PciI ACATGT 1 cut(s) 584
PciSI GCTCTTC 1 cut(s) 378
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 5 cut(s) 55, 166, 214, 239, 338
PkrI GCNGC 3 cut(s) 146, 260, 481
PleI GAGTC 5 cut(s) 24, 187, 863, 953, 1013
PpsI GAGTC 5 cut(s) 24, 187, 863, 953, 1013
Ppu21I YACGTR 1 cut(s) 886
PscI ACATGT 1 cut(s) 584
PsiI TTATAA 1 cut(s) 156
Psp6I CCWGG 4 cut(s) 64, 87, 522, 1099
PspFI CCCAGC 1 cut(s) 636
PspGI CCWGG 4 cut(s) 64, 87, 522, 1099
PspN4I GGNNCC 2 cut(s) 634, 700
PsuI RGATCY 2 cut(s) 997, 1094
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 4 cut(s) 222, 550, 772, 888
RsaNI GTAC 4 cut(s) 221, 549, 771, 887
RseI CAYNNNNRTG 1 cut(s) 1078
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 2 cut(s) 252, 1117
SatI GCNGC 3 cut(s) 145, 259, 480
Sau3AI GATC 4 cut(s) 766, 787, 997, 1094
SchI GAGTC 5 cut(s) 25, 188, 863, 953, 1013
ScrFI CCNGG 4 cut(s) 66, 89, 524, 1101
SduI GDGCHC 1 cut(s) 825
SfaNI GCATC 1 cut(s) 700
SmiMI CAYNNNNRTG 1 cut(s) 1078
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
SnaBI TACGTA 1 cut(s) 886
Sse9I AATT 3 cut(s) 150, 276, 964
SseBI AGGCCT 1 cut(s) 207
SsiI CCGC 1 cut(s) 256
SspMI CTAG 2 cut(s) 368, 1004
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 4 cut(s) 64, 87, 522, 1099
StyI CCWWGG 1 cut(s) 703
TaaI ACNGT 3 cut(s) 334, 775, 921
TaiI ACGT 2 cut(s) 888, 1082
TasI AATT 3 cut(s) 150, 276, 964
TatI WGTACW 2 cut(s) 220, 548
TfiI GAWTC 5 cut(s) 55, 166, 214, 239, 338
Tru1I TTAA 2 cut(s) 252, 1117
Tru9I TTAA 2 cut(s) 252, 1117
TscAI CASTG 4 cut(s) 780, 830, 926, 1096
TseFI GTSAC 2 cut(s) 347, 775
TseI GCWGC 3 cut(s) 144, 258, 479
Tsp45I GTSAC 2 cut(s) 347, 775
TspDTI ATGAA 4 cut(s) 521, 568, 677, 936
TspGWI ACGGA 1 cut(s) 552
TspRI CASTG 4 cut(s) 780, 830, 926, 1096
VneI GTGCAC 1 cut(s) 821
XapI RAATTY 2 cut(s) 150, 276
XceI RCATGY 1 cut(s) 588
XmiI GTMKAC 3 cut(s) 129, 473, 682
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 2 cut(s) 368, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.