pycom05g01840

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014656
Physical Location & Seq
Forward (+)
2189504 .. 2195683
6180 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01840.6

Sequence Viewer

Length: 1065 bp
ATGCTAGTGGCGAAGAGTCTGAGCAAGGCAGTAGTGCCGTCCTCTCTCATAGAGAATCCTTCTCCTGGTAGCCTCCAGTCCACTCGCCTCGCTCTCCATGTTGCCGAGGACTGCTCCTCGTGTTGGGTCTACATCGCCTCCGGCTGCCAAATTTATAAGCTTCAGATTCCGTTGGAAGAGTCTCTGGTCGGTGAAGGGAAAGAAGGCCTTCTGATTCCTGTACTCAGGGAGGTTATGGATTCCTCTATGGTTAACCGCTGCCCTCATCGTTCAGAAATTCAGAGTATGGTACTTGCTGAAACAGAGAGCAATGAATATTCAATGTTGGGAACTGTGGATTCTTATGGTCACCTTATTGTATCAAAACTAGATACTACTGGTGAAGAGCGGCTTACTTATTCAGTATTGCCTCGTGATTGTGGCATTGGAGAAAGTGGTTGGGCAGGGCTTTCCTTCAGTCCAGCACAGTGGTCTACGGCAGCCGTTGCGCGTAGCTTCTGCAAAACCATTGATGTTTATGACCAGGATATTCATGTCAGGACATTGTGTACACTTTGGTATCCGTCATCATTGAACTTCATAACAAGTTCGCATTATGGAAATGAGGGTTCTATATTAGCAATCACAGAAGGTTCCCAGCTGACTATATGGGACTTGAGAATGAAAGAAAATGGTGGCTGTCTACAACGAATATGTGGTTCCCTTGGTGATGCCTTTTATGCTGTGTGCTGTTCTTCAACTGGGAATGTTGCAGTCGGCGGAGCTGATCGTACAGTGACAATCTACGATCCTCGCAGATGGTCATCATTATCAAGATGGGTGCACTGCTCCAAATATGAGATAACTGGGCTTGCTTTCTCTTCAGTTGACTCCAACTACATCTACGTACAAGGTGTTGACTATGAGGTCTTATGTGGACAGTGGAAAGATAGCAGAAAGTTATCTTCATTTAGAGGAGACTCAAACTGGCTTGGTTTTAGCAAGTGCCAAAATAGGGATCTTCTAGGTGGATGGTGTGACTCTGGTAGTATCTTTGTAGCCGATGTTGTAGCTAAAGAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

355

Amino Acids

38.83

Weight (kDa)

5.15

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014656)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13700 FvH4_2g13700 FvH4_2g13700
malus_domestica MD05G1026600.v1.1
prunus_persica Prupe.8G031100_v2.0.a1
pyrus_communis pycom05g01840
rosa_chinensis RchiOBHm_Chr6g0275381
rosa_laevigata RLG00000013470
rosa_multiflora Rmu_sc0004143.1_g000003
rosa_roxburghii Rroxscaffold_7G00193370
rosa_rugosa Rorug06G0091200 Rorug06G0091200 Rorug06G0091200
rosa_samantha Rh6AG205100 Rh6BG207900 Rh6CG210300
rosa_wichuraiana Rw6G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
AasI GACNNNNNNGTC 1 cut(s) 905
AccBSI CCGCTC 1 cut(s) 388
AccI GTMKAC 3 cut(s) 129, 473, 682
AccII CGCG 1 cut(s) 490
AciI CCGC 3 cut(s) 256, 388, 759
AclWI GGATC 2 cut(s) 782, 1005
AcsI RAATTY 2 cut(s) 150, 276
AcuI CTGAAG 3 cut(s) 146, 439, 846
AfaI GTAC 5 cut(s) 222, 291, 550, 772, 888
AfiI CCNNNNNNNGG 3 cut(s) 65, 123, 994
AgsI TTSAA 3 cut(s) 321, 574, 738
AjnI CCWGG 2 cut(s) 64, 522
AluBI AGCT 5 cut(s) 160, 495, 640, 764, 1052
AluI AGCT 5 cut(s) 160, 495, 640, 764, 1052
Alw21I GWGCWC 1 cut(s) 825
Alw26I GTCTC 2 cut(s) 186, 951
Alw44I GTGCAC 1 cut(s) 821
AlwI GGATC 2 cut(s) 782, 1005
AoxI GGCC 1 cut(s) 205
ApaLI GTGCAC 1 cut(s) 821
ApeKI GCWGC 3 cut(s) 144, 258, 479
ApoI RAATTY 2 cut(s) 150, 276
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 490
AsuHPI GGTGA 4 cut(s) 203, 341, 392, 719
BaeGI GKGCMC 1 cut(s) 825
BaeI ACNNNNGTAYC 2 cut(s) 542, 575
BauI CACGAG 2 cut(s) 118, 411
Bbv12I GWGCWC 1 cut(s) 825
BbvI GCAGC 3 cut(s) 131, 245, 491
BccI CCATC 3 cut(s) 792, 810, 1005
BceAI ACGGC 3 cut(s) 22, 467, 492
BciT130I CCWGG 2 cut(s) 66, 524
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 186, 951
BfaI CTAG 3 cut(s) 5, 368, 1004
BfuI GTATCC 1 cut(s) 570
BisI GCNGC 4 cut(s) 145, 259, 389, 480
BlsI GCNGC 4 cut(s) 146, 260, 390, 481
Bme1390I CCNGG 2 cut(s) 66, 524
BmiI GGNNCC 2 cut(s) 634, 700
BmrFI CCNGG 2 cut(s) 66, 524
BmrI ACTGGG 2 cut(s) 750, 855
BmsI GCATC 1 cut(s) 700
BmuI ACTGGG 2 cut(s) 750, 855
BplI GAGNNNNNCTC 2 cut(s) 98, 130
BpmI CTGGAG 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 886
BsaBI GATNNNNATC 1 cut(s) 802
BsaJI CCNNGG 2 cut(s) 105, 703
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 123, 994
Bse1I ACTGG 5 cut(s) 76, 382, 745, 850, 971
Bse3DI GCAATG 1 cut(s) 316
Bse8I GATNNNNATC 1 cut(s) 802
BseBI CCWGG 2 cut(s) 66, 524
BseDI CCNNGG 2 cut(s) 105, 703
BseGI GGATG 1 cut(s) 1016
BseJI GATNNNNATC 1 cut(s) 802
BseLI CCNNNNNNNGG 3 cut(s) 65, 123, 994
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 2 cut(s) 11, 238
BseNI ACTGG 5 cut(s) 76, 382, 745, 850, 971
BseRI GAGGAG 2 cut(s) 106, 969
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 3 cut(s) 131, 245, 491
BseYI CCCAGC 1 cut(s) 636
Bsh1236I CGCG 1 cut(s) 490
BshFI GGCC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 825
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 3 cut(s) 65, 123, 994
BsmAI GTCTC 2 cut(s) 186, 951
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 825
Bsp1407I TGTACA 1 cut(s) 548
Bsp143I GATC 3 cut(s) 766, 787, 997
BspACI CCGC 3 cut(s) 256, 388, 759
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 2 cut(s) 12, 237
BspFNI CGCG 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 634, 700
BspPI GGATC 2 cut(s) 782, 1005
BspQI GCTCTTC 1 cut(s) 378
BsrBI CCGCTC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 316
BsrGI TGTACA 1 cut(s) 548
BsrI ACTGG 5 cut(s) 76, 382, 745, 850, 971
BssECI CCNNGG 2 cut(s) 105, 703
BssMI GATC 3 cut(s) 766, 787, 997
BssSI CACGAG 2 cut(s) 118, 411
BssT1I CCWWGG 1 cut(s) 703
Bst2BI CACGAG 2 cut(s) 118, 411
Bst2UI CCWGG 2 cut(s) 66, 524
Bst4CI ACNGT 4 cut(s) 334, 468, 775, 921
Bst6I CTCTTC 4 cut(s) 8, 171, 378, 865
BstAPI GCANNNNNTGC 1 cut(s) 485
BstAUI TGTACA 1 cut(s) 548
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 1 cut(s) 852
BstDEI CTNAG 2 cut(s) 20, 224
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 1 cut(s) 1016
BstFNI CGCG 1 cut(s) 490
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 3 cut(s) 769, 790, 1000
BstMAI GTCTC 2 cut(s) 186, 951
BstMBI GATC 3 cut(s) 766, 787, 997
BstMWI GCNNNNNNNGC 2 cut(s) 485, 719
BstNI CCWGG 2 cut(s) 66, 524
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 64, 522
BstSLI GKGCMC 1 cut(s) 825
BstSNI TACGTA 1 cut(s) 886
BstUI CGCG 1 cut(s) 490
BstV1I GCAGC 3 cut(s) 131, 245, 491
BstX2I RGATCY 1 cut(s) 997
BstXI CCANNNNNNTGG 1 cut(s) 468
BstYI RGATCY 1 cut(s) 997
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 1016
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 4 cut(s) 473, 780, 823, 926
Cac8I GCNNGC 1 cut(s) 852
CfoI GCGC 1 cut(s) 490
Csp6I GTAC 5 cut(s) 221, 290, 549, 771, 887
CviAII CATG 2 cut(s) 98, 533
CviQI GTAC 5 cut(s) 221, 290, 549, 771, 887
DdeI CTNAG 2 cut(s) 20, 224
DpnI GATC 3 cut(s) 768, 789, 999
DpnII GATC 3 cut(s) 766, 787, 997
DrdI GACNNNNNNGTC 1 cut(s) 905
DseDI GACNNNNNNGTC 1 cut(s) 905
Eam1104I CTCTTC 4 cut(s) 8, 171, 378, 865
EarI CTCTTC 4 cut(s) 8, 171, 378, 865
EciI GGCGGA 1 cut(s) 774
Eco105I TACGTA 1 cut(s) 886
Eco130I CCWWGG 1 cut(s) 703
Eco147I AGGCCT 1 cut(s) 207
Eco57I CTGAAG 3 cut(s) 146, 439, 846
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 2 cut(s) 64, 522
EcoT14I CCWWGG 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 703
FaeI CATG 2 cut(s) 101, 536
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 375, 407
FaqI GGGAC 1 cut(s) 665
FatI CATG 2 cut(s) 97, 532
FblI GTMKAC 3 cut(s) 129, 473, 682
Fnu4HI GCNGC 4 cut(s) 145, 259, 389, 480
FokI GGATG 1 cut(s) 1023
Fsp4HI GCNGC 4 cut(s) 145, 259, 389, 480
FspBI CTAG 3 cut(s) 5, 368, 1004
GlaI GCGC 1 cut(s) 489
GluI GCNGC 4 cut(s) 145, 259, 389, 480
GsaI CCCAGC 1 cut(s) 640
GsuI CTGGAG 1 cut(s) 59
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 141
HhaI GCGC 1 cut(s) 490
Hin1II CATG 2 cut(s) 101, 536
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HincII GTYRAC 3 cut(s) 253, 868, 898
HindII GTYRAC 3 cut(s) 253, 868, 898
HindIII AAGCTT 1 cut(s) 158
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 1 cut(s) 141
HphI GGTGA 4 cut(s) 203, 341, 392, 719
Hpy188I TCNGA 5 cut(s) 21, 165, 213, 274, 282
Hpy188III TCNNGA 3 cut(s) 413, 538, 813
HpyAV CCTTC 6 cut(s) 69, 188, 197, 218, 463, 623
HpyCH4III ACNGT 4 cut(s) 334, 468, 775, 921
HpyCH4IV ACGT 1 cut(s) 885
HpyCH4V TGCA 3 cut(s) 501, 752, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 485, 719
HpyF3I CTNAG 2 cut(s) 20, 224
HpySE526I ACGT 1 cut(s) 885
Hsp92II CATG 2 cut(s) 101, 536
HspAI GCGC 1 cut(s) 488
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 3 cut(s) 766, 787, 997
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 3 cut(s) 119, 761, 833
Lsp1109I GCAGC 3 cut(s) 131, 245, 491
LweI GCATC 1 cut(s) 700
MaeI CTAG 3 cut(s) 5, 368, 1004
MaeII ACGT 1 cut(s) 885
MaeIII GTNAC 3 cut(s) 347, 775, 1016
MalI GATC 3 cut(s) 768, 789, 999
MbiI CCGCTC 1 cut(s) 388
MboI GATC 3 cut(s) 766, 787, 997
MboII GAAGA 7 cut(s) 25, 188, 395, 726, 852, 936, 992
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 1 cut(s) 825
MluCI AATT 2 cut(s) 150, 276
MlyI GAGTC 5 cut(s) 25, 188, 863, 953, 1013
MmeI TCCRAC 2 cut(s) 153, 897
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 1 cut(s) 252
MspA1I CMGCKG 2 cut(s) 258, 640
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 2 cut(s) 66, 524
MvaI CCWGG 2 cut(s) 66, 524
MvnI CGCG 1 cut(s) 490
MwoI GCNNNNNNNGC 2 cut(s) 485, 719
NdeII GATC 3 cut(s) 766, 787, 997
NlaIII CATG 2 cut(s) 101, 536
NlaIV GGNNCC 2 cut(s) 634, 700
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 3 cut(s) 347, 775, 1016
PceI AGGCCT 1 cut(s) 207
PciSI GCTCTTC 1 cut(s) 378
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 5 cut(s) 55, 166, 214, 239, 338
PkrI GCNGC 4 cut(s) 146, 260, 390, 481
PleI GAGTC 5 cut(s) 24, 187, 863, 953, 1013
PpsI GAGTC 5 cut(s) 24, 187, 863, 953, 1013
Ppu21I YACGTR 1 cut(s) 886
PsiI TTATAA 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 64, 522
PspEI GGTNACC 1 cut(s) 347
PspFI CCCAGC 1 cut(s) 636
PspGI CCWGG 2 cut(s) 64, 522
PspN4I GGNNCC 2 cut(s) 634, 700
PsuI RGATCY 1 cut(s) 997
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 5 cut(s) 222, 291, 550, 772, 888
RsaNI GTAC 5 cut(s) 221, 290, 549, 771, 887
SapI GCTCTTC 1 cut(s) 378
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 4 cut(s) 145, 259, 389, 480
Sau3AI GATC 3 cut(s) 766, 787, 997
SchI GAGTC 5 cut(s) 25, 188, 863, 953, 1013
ScrFI CCNGG 2 cut(s) 66, 524
SduI GDGCHC 1 cut(s) 825
SfaNI GCATC 1 cut(s) 700
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
SnaBI TACGTA 1 cut(s) 886
Sse9I AATT 2 cut(s) 150, 276
SseBI AGGCCT 1 cut(s) 207
SsiI CCGC 3 cut(s) 256, 388, 759
SspI AATATT 1 cut(s) 317
SspMI CTAG 3 cut(s) 5, 368, 1004
StuI AGGCCT 1 cut(s) 207
StyD4I CCNGG 2 cut(s) 64, 522
StyI CCWWGG 1 cut(s) 703
TaaI ACNGT 4 cut(s) 334, 468, 775, 921
TaiI ACGT 1 cut(s) 888
TasI AATT 2 cut(s) 150, 276
TatI WGTACW 2 cut(s) 220, 548
TauI GCSGC 1 cut(s) 391
TfiI GAWTC 5 cut(s) 55, 166, 214, 239, 338
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 4 cut(s) 473, 780, 830, 926
TseFI GTSAC 3 cut(s) 347, 775, 1016
TseI GCWGC 3 cut(s) 144, 258, 479
Tsp45I GTSAC 3 cut(s) 347, 775, 1016
TspDTI ATGAA 5 cut(s) 327, 521, 568, 677, 936
TspGWI ACGGA 2 cut(s) 159, 552
TspRI CASTG 4 cut(s) 473, 780, 830, 926
VneI GTGCAC 1 cut(s) 821
XapI RAATTY 2 cut(s) 150, 276
XmiI GTMKAC 3 cut(s) 129, 473, 682
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 3 cut(s) 5, 368, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.