FvH4_2g14460

DUF218 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011939
Physical Location & Seq
Forward (+)
12720878 .. 12725397
4520 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14460.t2

Sequence Viewer

Length: 954 bp
ATGAGCAATAATCCTTATGGGTCTGGAAATCCTAAGTCCTTTGTTGCATACCCAAGTGGTGAGTTTGATTTAGAATCCGGAACCATTAGAAAAACCCGAAAGCATAGAAAATCAAAACCCAATCCCATCAAAATGATTAGGTCTGTTGGGAACCGCTTGAAGTACTATTGCAAGCTGCACCCTTGCTTAGTTTTCATGATCACTTTGTTAGTTGGGGTCACAATTCTTGTACTTTTGCCTCTGTATGAGAACCGGTTTCAAATGAGCAGCTATAAAAAATATGGCATGGATTTCGATAGCTATCCTCTGCCGATTCTCAAGAATCTTGTCATGGTTGCCGGGCATTCAGTTTATACTAGTAGTAGCTGTGGGAAAGTTGATAAAGAGGATTCTTGGTTTTTGGAGTCTTATCAGAAGAATCCTGGGCAAGCTTCTACTTTTTTGGCACATATACAAGAGGGAATTGAAACGGCCTCCAAAGACAATGCAGCTCTGCTTTTATTCAGTGGTGGAGAAACTCGGAGAGATGCCGGTCCTCGGAGTGAGGCACAGAGTTATTGGGCTGTTGCTGAATCAAAAGGATGGTTTGGCAAAGAAGATGTGAGACAGAGGGCACTCACAGAAGAGCATGCAAGGGACAGCTTTGAGAATCTTCTCTTCAGTGTGTGCCGGTTTCGAGAGCTTACTGGCATGTATCCTCAAAATATAACTGTTGTAAGTTATGATTTCAAGGAGGAGAGATTTGCGCATTTACATCGGTCTGCAATTGGCTTTCCTGAGTCGAGGTTCTTCTATGCTGGTACCCCAGCAATATCTACTGCAAAAGAAGCAGCTCGAAAAGGAGAGGCAGTGGTGCGGGCTCAGTTCCAAGAGGATCCATACGGGTGCCAAGGTTCACTTCGCCATAAGAAATTAGGGCGTGATCCATTTCACAGAAAAAAGCAAACTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF218

Protein Analysis

318

Amino Acids

36.0

Weight (kDa)

9.43

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21370 AT1G21370
fragaria_vesca FvH4_2g14460 FvH4_2g14460
malus_domestica MD05G1017500.v1.1 MD10G1017500.v1.1
prunus_persica Prupe.8G021400_v2.0.a1
pyrus_communis pycom05g00980 pycom10g01120
rosa_chinensis RchiOBHm_Chr6g0276791
rosa_laevigata RLG00000013361
rosa_multiflora Rmu_co8479231.1_g000001
rosa_roxburghii Rroxscaffold_7G00191940
rosa_rugosa Rorug06G0104500
rosa_samantha Rh6AG214400 Rh6BG219200 Rh6CG221900 Rh6DG211900
rosa_wichuraiana Rw6G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 747
Acc65I GGTACC 1 cut(s) 800
AccB1I GGYRCC 2 cut(s) 800, 885
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 2 cut(s) 154, 856
AclWI GGATC 3 cut(s) 869, 882, 917
AcuI CTGAAG 1 cut(s) 643
AfaI GTAC 3 cut(s) 164, 231, 802
AfiI CCNNNNNNNGG 1 cut(s) 537
AgeI ACCGGT 1 cut(s) 252
AgsI TTSAA 4 cut(s) 160, 260, 467, 730
AhlI ACTAGT 1 cut(s) 356
AjnI CCWGG 1 cut(s) 421
AjuI GAANNNNNNNTTGG 2 cut(s) 882, 914
AluBI AGCT 9 cut(s) 175, 270, 300, 366, 431, 491, 642, 682, 833
AluI AGCT 9 cut(s) 175, 270, 300, 366, 431, 491, 642, 682, 833
Alw26I GTCTC 1 cut(s) 598
AlwI GGATC 3 cut(s) 869, 882, 917
Aor13HI TCCGGA 1 cut(s) 77
AoxI GGCC 1 cut(s) 471
ApeKI GCWGC 4 cut(s) 175, 267, 488, 830
AsiGI ACCGGT 1 cut(s) 252
Asp718I GGTACC 1 cut(s) 800
AspLEI GCGC 1 cut(s) 748
AspS9I GGNCC 1 cut(s) 533
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 1 cut(s) 71
AvaII GGWCC 1 cut(s) 533
BaeGI GKGCMC 1 cut(s) 616
BamHI GGATCC 1 cut(s) 874
BanI GGYRCC 2 cut(s) 800, 885
BanII GRGCYC 1 cut(s) 862
BbvI GCAGC 4 cut(s) 162, 279, 500, 842
BccI CCATC 2 cut(s) 134, 576
BceAI ACGGC 1 cut(s) 486
BcgI CGANNNNNNTGC 4 cut(s) 274, 308, 737, 771
BciT130I CCWGG 1 cut(s) 423
BciVI GTATCC 1 cut(s) 705
BclI TGATCA 1 cut(s) 198
BcnI CCSGG 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 598
BcuI ACTAGT 1 cut(s) 356
BfaI CTAG 1 cut(s) 357
BfuI GTATCC 1 cut(s) 705
BisI GCNGC 4 cut(s) 176, 268, 489, 831
BlsI GCNGC 4 cut(s) 177, 269, 490, 832
BmcAI AGTACT 1 cut(s) 164
Bme1390I CCNGG 2 cut(s) 340, 423
Bme18I GGWCC 1 cut(s) 533
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 5 cut(s) 82, 152, 802, 876, 887
BmrFI CCNGG 2 cut(s) 340, 423
BmsI GCATC 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 340
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 3 cut(s) 422, 536, 889
BsaWI WCCGGW 2 cut(s) 77, 252
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 537
Bse118I RCCGGY 3 cut(s) 252, 530, 669
Bse1I ACTGG 1 cut(s) 691
Bse8I GATNNNNATC 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 77
BseBI CCWGG 1 cut(s) 423
BseDI CCNNGG 3 cut(s) 422, 536, 889
BseGI GGATG 1 cut(s) 587
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 537
BseMII CTCAG 2 cut(s) 768, 875
BseNI ACTGG 1 cut(s) 691
BseRI GAGGAG 1 cut(s) 749
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 4 cut(s) 162, 279, 500, 842
BseYI CCCAGC 1 cut(s) 805
BsgI GTGCAG 1 cut(s) 161
BshFI GGCC 1 cut(s) 473
BshNI GGYRCC 2 cut(s) 800, 885
BshTI ACCGGT 1 cut(s) 252
BsiSI CCGG 5 cut(s) 78, 253, 339, 531, 670
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 1 cut(s) 537
BsmAI GTCTC 1 cut(s) 598
BsmFI GGGAC 1 cut(s) 650
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 616, 862
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 3 cut(s) 198, 874, 922
BspACI CCGC 2 cut(s) 154, 856
BspANI GGCC 1 cut(s) 473
BspCNI CTCAG 2 cut(s) 769, 874
BspEI TCCGGA 1 cut(s) 77
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 5 cut(s) 82, 152, 802, 876, 887
BspPI GGATC 3 cut(s) 869, 882, 917
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 2 cut(s) 800, 885
BsrFI RCCGGY 3 cut(s) 252, 530, 669
BsrI ACTGG 1 cut(s) 691
BssAI RCCGGY 3 cut(s) 252, 530, 669
BssECI CCNNGG 3 cut(s) 422, 536, 889
BssMI GATC 3 cut(s) 198, 874, 922
BssT1I CCWWGG 1 cut(s) 889
Bst2UI CCWGG 1 cut(s) 423
Bst4CI ACNGT 1 cut(s) 712
Bst6I CTCTTC 2 cut(s) 618, 662
BstC8I GCNNGC 4 cut(s) 173, 429, 630, 858
BstDEI CTNAG 4 cut(s) 33, 187, 777, 861
BstF5I GGATG 1 cut(s) 587
BstHHI GCGC 1 cut(s) 748
BstKTI GATC 3 cut(s) 201, 877, 925
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 3 cut(s) 198, 874, 922
BstMWI GCNNNNNNNGC 1 cut(s) 827
BstNI CCWGG 1 cut(s) 423
BstNSI RCATGY 2 cut(s) 632, 694
BstSCI CCNGG 2 cut(s) 338, 421
BstSLI GKGCMC 1 cut(s) 616
BstV1I GCAGC 4 cut(s) 162, 279, 500, 842
BstX2I RGATCY 1 cut(s) 874
BstYI RGATCY 1 cut(s) 874
BsuI GTATCC 1 cut(s) 705
BsuRI GGCC 1 cut(s) 473
BtsCI GGATG 1 cut(s) 587
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 3 cut(s) 511, 667, 855
Cac8I GCNNGC 4 cut(s) 173, 429, 630, 858
CciI TCATGA 1 cut(s) 195
CfoI GCGC 1 cut(s) 748
Cfr10I RCCGGY 3 cut(s) 252, 530, 669
Cfr13I GGNCC 1 cut(s) 533
Csp6I GTAC 3 cut(s) 163, 230, 801
CspAI ACCGGT 1 cut(s) 252
CviAII CATG 5 cut(s) 196, 286, 331, 629, 691
CviQI GTAC 3 cut(s) 163, 230, 801
DdeI CTNAG 4 cut(s) 33, 187, 777, 861
DpnI GATC 3 cut(s) 200, 876, 924
DpnII GATC 3 cut(s) 198, 874, 922
Eam1104I CTCTTC 2 cut(s) 618, 662
EarI CTCTTC 2 cut(s) 618, 662
Eco130I CCWWGG 1 cut(s) 889
Eco24I GRGCYC 1 cut(s) 862
Eco47I GGWCC 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 889
EcoT38I GRGCYC 1 cut(s) 862
ErhI CCWWGG 1 cut(s) 889
FaeI CATG 5 cut(s) 199, 289, 334, 632, 694
FalI AAGNNNNNCTT 2 cut(s) 882, 914
FaqI GGGAC 1 cut(s) 650
FatI CATG 5 cut(s) 195, 285, 330, 628, 690
FauI CCCGC 1 cut(s) 849
FbaI TGATCA 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 176, 268, 489, 831
FokI GGATG 1 cut(s) 594
FriOI GRGCYC 1 cut(s) 862
Fsp4HI GCNGC 4 cut(s) 176, 268, 489, 831
FspBI CTAG 1 cut(s) 357
FspI TGCGCA 1 cut(s) 747
GlaI GCGC 1 cut(s) 747
GluI GCNGC 4 cut(s) 176, 268, 489, 831
GsaI CCCAGC 1 cut(s) 809
HaeIII GGCC 1 cut(s) 473
HapII CCGG 5 cut(s) 78, 253, 339, 531, 670
HhaI GCGC 1 cut(s) 748
Hin1II CATG 5 cut(s) 199, 289, 334, 632, 694
Hin6I GCGC 1 cut(s) 746
HinP1I GCGC 1 cut(s) 746
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 9 cut(s) 74, 313, 322, 389, 404, 418, 572, 649, 779
HpaII CCGG 5 cut(s) 78, 253, 339, 531, 670
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 896
Hpy188I TCNGA 3 cut(s) 414, 522, 540
Hpy188III TCNNGA 6 cut(s) 24, 78, 196, 319, 677, 776
Hpy8I GTNNAC 1 cut(s) 896
HpyCH4III ACNGT 1 cut(s) 712
HpyCH4V TGCA 7 cut(s) 47, 171, 178, 488, 632, 764, 821
HpyF10VI GCNNNNNNNGC 1 cut(s) 827
HpyF3I CTNAG 4 cut(s) 33, 187, 777, 861
Hsp92II CATG 5 cut(s) 199, 289, 334, 632, 694
HspAI GCGC 1 cut(s) 746
Kpn2I TCCGGA 1 cut(s) 77
KpnI GGTACC 1 cut(s) 804
Ksp22I TGATCA 1 cut(s) 198
Kzo9I GATC 3 cut(s) 198, 874, 922
LguI GCTCTTC 1 cut(s) 618
Lsp1109I GCAGC 4 cut(s) 162, 279, 500, 842
LweI GCATC 1 cut(s) 517
MaeI CTAG 1 cut(s) 357
MaeIII GTNAC 1 cut(s) 217
MalI GATC 3 cut(s) 200, 876, 924
MboI GATC 3 cut(s) 198, 874, 922
MboII GAAGA 6 cut(s) 427, 608, 635, 644, 649, 781
MfeI CAATTG 1 cut(s) 765
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 2 cut(s) 616, 862
MluCI AATT 4 cut(s) 222, 462, 765, 911
MlyI GAGTC 2 cut(s) 413, 788
MroI TCCGGA 1 cut(s) 77
MslI CAYNNNNRTG 2 cut(s) 131, 883
MspI CCGG 5 cut(s) 78, 253, 339, 531, 670
MspR9I CCNGG 2 cut(s) 340, 423
MunI CAATTG 1 cut(s) 765
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 1 cut(s) 423
MwoI GCNNNNNNNGC 1 cut(s) 827
NciI CCSGG 1 cut(s) 340
NdeII GATC 3 cut(s) 198, 874, 922
NlaIII CATG 5 cut(s) 199, 289, 334, 632, 694
NlaIV GGNNCC 5 cut(s) 82, 152, 802, 876, 887
NmuCI GTSAC 1 cut(s) 217
NsbI TGCGCA 1 cut(s) 747
NspI RCATGY 2 cut(s) 632, 694
PaeI GCATGC 1 cut(s) 632
PagI TCATGA 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 618
PctI GAATGC 1 cut(s) 343
PfeI GAWTC 7 cut(s) 74, 313, 322, 389, 418, 572, 649
PinAI ACCGGT 1 cut(s) 252
PkrI GCNGC 4 cut(s) 177, 269, 490, 832
PleI GAGTC 2 cut(s) 412, 787
PpsI GAGTC 2 cut(s) 412, 787
Psp6I CCWGG 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 805
PspGI CCWGG 1 cut(s) 421
PspN4I GGNNCC 5 cut(s) 82, 152, 802, 876, 887
PspPI GGNCC 1 cut(s) 533
PsuI RGATCY 1 cut(s) 874
RsaI GTAC 3 cut(s) 164, 231, 802
RsaNI GTAC 3 cut(s) 163, 230, 801
RseI CAYNNNNRTG 2 cut(s) 131, 883
SapI GCTCTTC 1 cut(s) 618
SatI GCNGC 4 cut(s) 176, 268, 489, 831
Sau3AI GATC 3 cut(s) 198, 874, 922
Sau96I GGNCC 1 cut(s) 533
ScaI AGTACT 1 cut(s) 164
SchI GAGTC 2 cut(s) 413, 788
ScrFI CCNGG 2 cut(s) 340, 423
SduI GDGCHC 2 cut(s) 616, 862
SfaNI GCATC 1 cut(s) 517
SinI GGWCC 1 cut(s) 533
SmiMI CAYNNNNRTG 2 cut(s) 131, 883
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SpeI ACTAGT 1 cut(s) 356
SphI GCATGC 1 cut(s) 632
Sse9I AATT 4 cut(s) 222, 462, 765, 911
SsiI CCGC 2 cut(s) 154, 856
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 2 cut(s) 338, 421
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 1 cut(s) 712
TaqI TCGA 4 cut(s) 294, 676, 782, 835
TaqII GACCGA 1 cut(s) 747
TasI AATT 4 cut(s) 222, 462, 765, 911
TatI WGTACW 2 cut(s) 162, 229
TfiI GAWTC 7 cut(s) 74, 313, 322, 389, 418, 572, 649
TscAI CASTG 3 cut(s) 511, 667, 855
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 4 cut(s) 175, 267, 488, 830
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 184
TspRI CASTG 3 cut(s) 511, 667, 855
VpaK11BI GGWCC 1 cut(s) 533
XceI RCATGY 2 cut(s) 632, 694
XspI CTAG 1 cut(s) 357
ZrmI AGTACT 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.