Prupe.8G021400_v2.0.a1

DUF218 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0011939
Physical Location & Seq
Reverse (-)
1924260 .. 1929204
4945 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G021400.1

Sequence Viewer

Length: 1038 bp
ATGAGCAATTATCCATTTCGGCCTGGCAGTCCTAAATCCTTCACAGCCTACCCAAGAGGTGACTTTGATTTAGAATCTGGAACCATTAGAAGAACCCGAAAGCATAGGCATTCACCATTGCAACCCATCAAAATGATTAGGTCCATTGGAAACCGCCTTCAGTATTATTGTAAGCTGCACCCACTTTTAGTTTTCACCATCTCCTTGTCAGTTGGGGTCACAATTCTCTTACTTTTCTCTCTATATGAGAGCCGGTTTGAAATGATGGCTAGCTATAAAAAATTTGACACGGGTTCGAATAGTTATCCGTTGCCGAATCTTCAGAATCTAGTCATGGTTGCCGGGCATTCAGTTTATACTAGCAGTAGCTGTGGAAAAGTTGATAAGGAGGATTCTTGGTTTTTGGAGTCTTATCAGAAGAATCCAGGTCAAGCTGCAACTTTCGTGGCGCATATACAAGAGGGTATTGAAGTCGCAGCCAAAGACGATAGAGCTTTGCTTCTGTTTAGTGGTGGAGAGACTCGAAGAGATGCTGGTCCTCGGAGCGAGGCACAGAGTTATTGGGCAGTTGCTGAATCAAAAGGATGGTTTGGCAAAGAAGATTTGAGATCAAGGACACTCACAGAAGAGCATGCAAGGGACAGTTTTGAGAATCTTCTATTCAGTGTGTGCCGGTTCCGAGAACTTACTGGCACATATCCTCGTAATATAACTGTTGTTAGTTATGATTTCAAGGAGGAGAGATTTGCACATCTACATCGGTCTGCAATTGGCTTTCCAGAGTCAAGGTTCTTCTATGCCGGTACCCCAGCAATTTCTACTGCAAAAGAAGCAGCTCGAAAAGGAGAAGCAATTGTGCGTGCTCAATTCCAAGATGATCCATATGGCTGTCGAGGTTCACTTTACCATAAAAAACTAGGGCGTGATCCGTTTCACCGGTCTATTCCCTATCCAAATGGGTGTCCTGAAATTGAAGGTCTTTTCAGATATTGCGGGACAGATCCTTATCCAGGGTCCCTTCCCTGGCCTAAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF218

Protein Analysis

346

Amino Acids

39.18

Weight (kDa)

9.15

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21370 AT1G21370
fragaria_vesca FvH4_2g14460 FvH4_2g14460
malus_domestica MD05G1017500.v1.1 MD10G1017500.v1.1
prunus_persica Prupe.8G021400_v2.0.a1
pyrus_communis pycom05g00980 pycom10g01120
rosa_chinensis RchiOBHm_Chr6g0276791
rosa_laevigata RLG00000013361
rosa_multiflora Rmu_co8479231.1_g000001
rosa_roxburghii Rroxscaffold_7G00191940
rosa_rugosa Rorug06G0104500
rosa_samantha Rh6AG214400 Rh6BG219200 Rh6CG221900 Rh6DG211900
rosa_wichuraiana Rw6G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 803
AccB1I GGYRCC 1 cut(s) 803
AciI CCGC 2 cut(s) 154, 993
AclWI GGATC 3 cut(s) 872, 920, 995
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 2 cut(s) 143, 305
AfaI GTAC 1 cut(s) 805
AfiI CCNNNNNNNGG 2 cut(s) 1010, 1023
AgeI ACCGGT 1 cut(s) 936
AgsI TTSAA 4 cut(s) 260, 470, 733, 974
AjnI CCWGG 4 cut(s) 22, 424, 1009, 1022
AluBI AGCT 6 cut(s) 175, 273, 369, 434, 494, 836
AluI AGCT 6 cut(s) 175, 273, 369, 434, 494, 836
Alw21I GWGCWC 1 cut(s) 865
Alw26I GTCTC 1 cut(s) 512
AlwI GGATC 3 cut(s) 872, 920, 995
AlwNI CAGNNNCTG 2 cut(s) 369, 572
AoxI GGCC 2 cut(s) 20, 1025
ApeKI GCWGC 4 cut(s) 175, 434, 476, 833
ApoI RAATTY 1 cut(s) 281
AsiGI ACCGGT 1 cut(s) 936
Asp718I GGTACC 1 cut(s) 803
AspLEI GCGC 1 cut(s) 451
AspS9I GGNCC 3 cut(s) 141, 536, 1014
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 4 cut(s) 71, 105, 187, 926
AsuII TTCGAA 1 cut(s) 296
AsuNHI GCTAGC 1 cut(s) 269
AvaII GGWCC 3 cut(s) 141, 536, 1014
BanI GGYRCC 1 cut(s) 803
Bbv12I GWGCWC 1 cut(s) 865
BbvI GCAGC 4 cut(s) 162, 421, 488, 845
BccI CCATC 4 cut(s) 134, 206, 259, 579
BciT130I CCWGG 4 cut(s) 24, 426, 1011, 1024
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 512
BfaI CTAG 4 cut(s) 270, 329, 360, 917
BisI GCNGC 4 cut(s) 176, 435, 477, 834
BlsI GCNGC 4 cut(s) 177, 436, 478, 835
Bme1390I CCNGG 5 cut(s) 24, 343, 426, 1011, 1024
Bme18I GGWCC 3 cut(s) 141, 536, 1014
BmgT120I GGNCC 3 cut(s) 141, 536, 1014
BmiI GGNNCC 5 cut(s) 82, 677, 805, 1015, 1016
BmrFI CCNGG 5 cut(s) 24, 343, 426, 1011, 1024
BmsI GCATC 1 cut(s) 520
BmtI GCTAGC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 343
BsaBI GATNNNNATC 1 cut(s) 1005
BsaJI CCNNGG 3 cut(s) 539, 1010, 1022
BsaWI WCCGGW 1 cut(s) 936
Bsc4I CCNNNNNNNGG 2 cut(s) 1010, 1023
Bse118I RCCGGY 4 cut(s) 252, 672, 800, 936
Bse1I ACTGG 1 cut(s) 694
Bse3DI GCAATG 1 cut(s) 116
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 4 cut(s) 24, 426, 1011, 1024
BseDI CCNNGG 3 cut(s) 539, 1010, 1022
BseGI GGATG 1 cut(s) 590
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 2 cut(s) 1010, 1023
BseMI GCAATG 1 cut(s) 116
BseNI ACTGG 1 cut(s) 694
BseRI GAGGAG 1 cut(s) 752
BseXI GCAGC 4 cut(s) 162, 421, 488, 845
BseYI CCCAGC 1 cut(s) 808
BsgI GTGCAG 1 cut(s) 161
BshFI GGCC 2 cut(s) 22, 1027
BshNI GGYRCC 1 cut(s) 803
BshTI ACCGGT 1 cut(s) 936
BsiHKAI GWGCWC 1 cut(s) 865
BsiSI CCGG 5 cut(s) 253, 342, 673, 801, 937
BslFI GGGAC 3 cut(s) 653, 1000, 1009
BslI CCNNNNNNNGG 2 cut(s) 1010, 1023
BsmAI GTCTC 1 cut(s) 512
BsmFI GGGAC 3 cut(s) 653, 1000, 1009
BsmI GAATGC 2 cut(s) 109, 346
BsnI GGCC 2 cut(s) 22, 1027
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 865
Bsp143I GATC 4 cut(s) 608, 877, 925, 1000
BspACI CCGC 2 cut(s) 154, 993
BspANI GGCC 2 cut(s) 22, 1027
BspLI GGNNCC 5 cut(s) 82, 677, 805, 1015, 1016
BspOI GCTAGC 1 cut(s) 273
BspPI GGATC 3 cut(s) 872, 920, 995
BspQI GCTCTTC 1 cut(s) 621
BspT104I TTCGAA 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 803
BsrDI GCAATG 1 cut(s) 116
BsrFI RCCGGY 4 cut(s) 252, 672, 800, 936
BsrI ACTGG 1 cut(s) 694
BssAI RCCGGY 4 cut(s) 252, 672, 800, 936
BssECI CCNNGG 3 cut(s) 539, 1010, 1022
BssMI GATC 4 cut(s) 608, 877, 925, 1000
Bst2UI CCWGG 4 cut(s) 24, 426, 1011, 1024
Bst4CI ACNGT 2 cut(s) 644, 715
Bst6I CTCTTC 2 cut(s) 520, 621
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 3 cut(s) 271, 633, 861
BstDEI CTNAG 1 cut(s) 1029
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstF5I GGATG 1 cut(s) 590
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 4 cut(s) 611, 880, 928, 1003
BstMAI GTCTC 1 cut(s) 512
BstMBI GATC 4 cut(s) 608, 877, 925, 1000
BstMWI GCNNNNNNNGC 1 cut(s) 830
BstNI CCWGG 4 cut(s) 24, 426, 1011, 1024
BstNSI RCATGY 1 cut(s) 635
BstSCI CCNGG 5 cut(s) 22, 341, 424, 1009, 1022
BstV1I GCAGC 4 cut(s) 162, 421, 488, 845
BstX2I RGATCY 1 cut(s) 1000
BstYI RGATCY 1 cut(s) 1000
BsuRI GGCC 2 cut(s) 22, 1027
BtsCI GGATG 1 cut(s) 590
BtsIMutI CAGTG 1 cut(s) 670
Cac8I GCNNGC 3 cut(s) 271, 633, 861
CaiI CAGNNNCTG 2 cut(s) 369, 572
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 4 cut(s) 252, 672, 800, 936
Cfr13I GGNCC 3 cut(s) 141, 536, 1014
Csp6I GTAC 1 cut(s) 804
CspAI ACCGGT 1 cut(s) 936
CspCI CAANNNNNGTGG 2 cut(s) 426, 461
CviAII CATG 2 cut(s) 334, 632
CviQI GTAC 1 cut(s) 804
DdeI CTNAG 1 cut(s) 1029
DpnI GATC 4 cut(s) 610, 879, 927, 1002
DpnII GATC 4 cut(s) 608, 877, 925, 1000
Eam1104I CTCTTC 2 cut(s) 520, 621
EarI CTCTTC 2 cut(s) 520, 621
Eco47I GGWCC 3 cut(s) 141, 536, 1014
Eco57I CTGAAG 2 cut(s) 143, 305
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoO109I RGGNCCY 1 cut(s) 1014
EcoRII CCWGG 4 cut(s) 22, 424, 1009, 1022
FaeI CATG 2 cut(s) 337, 635
FaqI GGGAC 3 cut(s) 653, 1000, 1009
FatI CATG 2 cut(s) 333, 631
FauI CCCGC 1 cut(s) 986
FauNDI CATATG 1 cut(s) 883
Fnu4HI GCNGC 4 cut(s) 176, 435, 477, 834
FokI GGATG 1 cut(s) 597
Fsp4HI GCNGC 4 cut(s) 176, 435, 477, 834
FspBI CTAG 4 cut(s) 270, 329, 360, 917
GlaI GCGC 1 cut(s) 450
GluI GCNGC 4 cut(s) 176, 435, 477, 834
GsaI CCCAGC 1 cut(s) 812
HaeIII GGCC 2 cut(s) 22, 1027
HapII CCGG 5 cut(s) 253, 342, 673, 801, 937
HhaI GCGC 1 cut(s) 451
Hin1II CATG 2 cut(s) 337, 635
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HpaII CCGG 5 cut(s) 253, 342, 673, 801, 937
HphI GGTGA 4 cut(s) 71, 105, 187, 926
Hpy166II GTNNAC 1 cut(s) 899
Hpy188I TCNGA 5 cut(s) 324, 417, 543, 680, 986
Hpy188III TCNNGA 3 cut(s) 78, 779, 965
Hpy8I GTNNAC 1 cut(s) 899
HpyAV CCTTC 4 cut(s) 49, 167, 968, 1028
HpyCH4III ACNGT 2 cut(s) 644, 715
HpyCH4V TGCA 7 cut(s) 121, 178, 437, 635, 749, 767, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 830
HpyF3I CTNAG 1 cut(s) 1029
Hsp92II CATG 2 cut(s) 337, 635
HspAI GCGC 1 cut(s) 449
KflI GGGWCCC 1 cut(s) 1014
KpnI GGTACC 1 cut(s) 807
Kzo9I GATC 4 cut(s) 608, 877, 925, 1000
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 1 cut(s) 543
Lsp1109I GCAGC 4 cut(s) 162, 421, 488, 845
LweI GCATC 1 cut(s) 520
MaeI CTAG 4 cut(s) 270, 329, 360, 917
MaeIII GTNAC 2 cut(s) 59, 217
MalI GATC 4 cut(s) 610, 879, 927, 1002
MboI GATC 4 cut(s) 608, 877, 925, 1000
MboII GAAGA 8 cut(s) 102, 311, 430, 537, 611, 638, 647, 784
MfeI CAATTG 2 cut(s) 768, 852
MflI RGATCY 1 cut(s) 1000
MhlI GDGCHC 1 cut(s) 865
MluCI AATT 8 cut(s) 7, 222, 281, 768, 813, 852, 866, 969
MlyI GAGTC 3 cut(s) 416, 514, 791
MnlI CCTC 8 cut(s) 50, 382, 454, 541, 549, 711, 730, 887
MseI TTAA 1 cut(s) 1036
MslI CAYNNNNRTG 1 cut(s) 131
MspI CCGG 5 cut(s) 253, 342, 673, 801, 937
MspR9I CCNGG 5 cut(s) 24, 343, 426, 1011, 1024
MunI CAATTG 2 cut(s) 768, 852
Mva1269I GAATGC 2 cut(s) 109, 346
MvaI CCWGG 4 cut(s) 24, 426, 1011, 1024
MwoI GCNNNNNNNGC 1 cut(s) 830
NciI CCSGG 1 cut(s) 343
NdeI CATATG 1 cut(s) 883
NdeII GATC 4 cut(s) 608, 877, 925, 1000
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 2 cut(s) 337, 635
NlaIV GGNNCC 5 cut(s) 82, 677, 805, 1015, 1016
NmuCI GTSAC 2 cut(s) 59, 217
NspI RCATGY 1 cut(s) 635
NspV TTCGAA 1 cut(s) 296
PaeI GCATGC 1 cut(s) 635
PciSI GCTCTTC 1 cut(s) 621
PctI GAATGC 2 cut(s) 109, 346
PfeI GAWTC 7 cut(s) 74, 316, 325, 392, 421, 575, 652
PinAI ACCGGT 1 cut(s) 936
PkrI GCNGC 4 cut(s) 177, 436, 478, 835
PleI GAGTC 3 cut(s) 415, 514, 790
PpsI GAGTC 3 cut(s) 415, 514, 790
PpuMI RGGWCCY 1 cut(s) 1014
Psp5II RGGWCCY 1 cut(s) 1014
Psp6I CCWGG 4 cut(s) 22, 424, 1009, 1022
PspFI CCCAGC 1 cut(s) 808
PspGI CCWGG 4 cut(s) 22, 424, 1009, 1022
PspN4I GGNNCC 5 cut(s) 82, 677, 805, 1015, 1016
PspPI GGNCC 3 cut(s) 141, 536, 1014
PspPPI RGGWCCY 1 cut(s) 1014
PstNI CAGNNNCTG 2 cut(s) 369, 572
PsuI RGATCY 1 cut(s) 1000
RsaI GTAC 1 cut(s) 805
RsaNI GTAC 1 cut(s) 804
RseI CAYNNNNRTG 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 1 cut(s) 1036
SatI GCNGC 4 cut(s) 176, 435, 477, 834
Sau3AI GATC 4 cut(s) 608, 877, 925, 1000
Sau96I GGNCC 3 cut(s) 141, 536, 1014
SchI GAGTC 3 cut(s) 416, 514, 791
ScrFI CCNGG 5 cut(s) 24, 343, 426, 1011, 1024
SduI GDGCHC 1 cut(s) 865
SfaNI GCATC 1 cut(s) 520
SfuI TTCGAA 1 cut(s) 296
SinI GGWCC 3 cut(s) 141, 536, 1014
SmiMI CAYNNNNRTG 1 cut(s) 131
SphI GCATGC 1 cut(s) 635
Sse9I AATT 8 cut(s) 7, 222, 281, 768, 813, 852, 866, 969
SsiI CCGC 2 cut(s) 154, 993
SspMI CTAG 4 cut(s) 270, 329, 360, 917
StyD4I CCNGG 5 cut(s) 22, 341, 424, 1009, 1022
TaaI ACNGT 2 cut(s) 644, 715
TaqI TCGA 4 cut(s) 296, 523, 838, 892
TaqII GACCGA 1 cut(s) 750
TasI AATT 8 cut(s) 7, 222, 281, 768, 813, 852, 866, 969
TfiI GAWTC 7 cut(s) 74, 316, 325, 392, 421, 575, 652
Tru1I TTAA 1 cut(s) 1036
Tru9I TTAA 1 cut(s) 1036
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 2 cut(s) 59, 217
TseI GCWGC 4 cut(s) 175, 434, 476, 833
Tsp45I GTSAC 2 cut(s) 59, 217
TspGWI ACGGA 2 cut(s) 297, 918
TspRI CASTG 1 cut(s) 670
VpaK11BI GGWCC 3 cut(s) 141, 536, 1014
XagI CCTNNNNNAGG 1 cut(s) 1008
XapI RAATTY 1 cut(s) 281
XceI RCATGY 1 cut(s) 635
XspI CTAG 4 cut(s) 270, 329, 360, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.