Rmu_co8479231.1_g000001

DUF218 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8479231.1
OG0011939
Physical Location & Seq
Reverse (-)
808 .. 1895
1088 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8479231.1_g000001.1.cds

Sequence Viewer

Length: 462 bp
atgatggtttgtttaggcaaagaagatctgagacggagggcactcacagaagagcatgctagggatagctttgagaatcttctctttagtgtttgccgatttcgagagcttactggcatgtatcctcataatataactgtcgtaagttatgatttcaaggaggagagatttgcgcatttacataggtctgcaattggctttccagagtcaaggttcttctatgctggtaccccagcaatttctactgcaaaagaagcagctcgaaaaggagaggcaatcgtgcgggctcagttccaagaggatccatacgggtgtcaaggttcacttcaccgcaaaaaactagggcgtgatccttttcaccggactattccttatcctaatggatgtcctgaaattgaaggtctattcaggtattgtgggactgctccttatccagggtccctcccttggtctcatacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF218

Protein Analysis

153

Amino Acids

17.54

Weight (kDa)

8.63

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21370 AT1G21370
fragaria_vesca FvH4_2g14460 FvH4_2g14460
malus_domestica MD05G1017500.v1.1 MD10G1017500.v1.1
prunus_persica Prupe.8G021400_v2.0.a1
pyrus_communis pycom05g00980 pycom10g01120
rosa_chinensis RchiOBHm_Chr6g0276791
rosa_laevigata RLG00000013361
rosa_multiflora Rmu_co8479231.1_g000001
rosa_roxburghii Rroxscaffold_7G00191940
rosa_rugosa Rorug06G0104500
rosa_samantha Rh6AG214400 Rh6BG219200 Rh6CG221900 Rh6DG211900
rosa_wichuraiana Rw6G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 174
Acc65I GGTACC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 227
AciI CCGC 2 cut(s) 283, 331
AclWI GGATC 3 cut(s) 296, 309, 344
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 2 cut(s) 434, 447
AgsI TTSAA 2 cut(s) 157, 398
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 3 cut(s) 69, 109, 260
AluI AGCT 3 cut(s) 69, 109, 260
Alw26I GTCTC 2 cut(s) 25, 456
AlwI GGATC 3 cut(s) 296, 309, 344
ApeKI GCWGC 1 cut(s) 257
Asp718I GGTACC 1 cut(s) 227
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 1 cut(s) 438
AsuHPI GGTGA 2 cut(s) 320, 350
AvaII GGWCC 1 cut(s) 438
BaeGI GKGCMC 1 cut(s) 43
BamHI GGATCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 227
BanII GRGCYC 1 cut(s) 289
BbvI GCAGC 1 cut(s) 269
BciT130I CCWGG 1 cut(s) 435
BciVI GTATCC 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 25, 456
BfaI CTAG 2 cut(s) 60, 341
BfuI GTATCC 1 cut(s) 132
BglII AGATCT 1 cut(s) 25
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 1 cut(s) 438
BmgT120I GGNCC 1 cut(s) 438
BmiI GGNNCC 4 cut(s) 229, 303, 439, 440
BmrFI CCNGG 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 2 cut(s) 434, 446
BsaWI WCCGGW 1 cut(s) 360
Bsc4I CCNNNNNNNGG 2 cut(s) 434, 447
Bse1I ACTGG 1 cut(s) 118
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 2 cut(s) 434, 446
BseGI GGATG 1 cut(s) 389
BseLI CCNNNNNNNGG 2 cut(s) 434, 447
BseMII CTCAG 2 cut(s) 20, 302
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 176
BseSI GKGCMC 1 cut(s) 43
BseXI GCAGC 1 cut(s) 269
BseYI CCCAGC 1 cut(s) 232
BshNI GGYRCC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 361
BslFI GGGAC 2 cut(s) 424, 433
BslI CCNNNNNNNGG 2 cut(s) 434, 447
BsmAI GTCTC 2 cut(s) 25, 456
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 2 cut(s) 424, 433
Bso31I GGTCTC 1 cut(s) 456
Bsp1286I GDGCHC 2 cut(s) 43, 289
Bsp143I GATC 3 cut(s) 25, 301, 349
BspACI CCGC 2 cut(s) 283, 331
BspCNI CTCAG 2 cut(s) 21, 301
BspLI GGNNCC 4 cut(s) 229, 303, 439, 440
BspPI GGATC 3 cut(s) 296, 309, 344
BspQI GCTCTTC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 456
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 434, 446
BssMI GATC 3 cut(s) 25, 301, 349
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 57, 285
BstDEI CTNAG 2 cut(s) 29, 288
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 389
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 3 cut(s) 28, 304, 352
BstMAI GTCTC 2 cut(s) 25, 456
BstMBI GATC 3 cut(s) 25, 301, 349
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 2 cut(s) 59, 121
BstSCI CCNGG 1 cut(s) 433
BstSLI GKGCMC 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 269
BstX2I RGATCY 2 cut(s) 25, 301
BstYI RGATCY 2 cut(s) 25, 301
BsuI GTATCC 1 cut(s) 132
BtsCI GGATG 1 cut(s) 389
Cac8I GCNNGC 2 cut(s) 57, 285
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 1 cut(s) 438
Csp6I GTAC 1 cut(s) 228
CviAII CATG 2 cut(s) 56, 118
CviJI RGCY 5 cut(s) 69, 109, 198, 260, 287
CviKI_1 RGCY 5 cut(s) 69, 109, 198, 260, 287
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 2 cut(s) 29, 288
DpnI GATC 3 cut(s) 27, 303, 351
DpnII GATC 3 cut(s) 25, 301, 349
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco130I CCWWGG 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 289
Eco31I GGTCTC 1 cut(s) 456
Eco47I GGWCC 1 cut(s) 438
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoO109I RGGNCCY 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 1 cut(s) 446
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 446
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 2 cut(s) 59, 121
FaiI YATR 9 cut(s) 57, 119, 129, 134, 150, 183, 222, 307, 456
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 2 cut(s) 424, 433
FatI CATG 2 cut(s) 55, 117
FauI CCCGC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 1 cut(s) 396
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 2 cut(s) 60, 341
FspI TGCGCA 1 cut(s) 174
GlaI GCGC 1 cut(s) 174
GluI GCNGC 1 cut(s) 258
GsaI CCCAGC 1 cut(s) 236
HapII CCGG 1 cut(s) 361
HhaI GCGC 1 cut(s) 175
Hin1II CATG 2 cut(s) 59, 121
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 2 cut(s) 76, 206
HpaII CCGG 1 cut(s) 361
HphI GGTGA 2 cut(s) 320, 350
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 3 cut(s) 104, 203, 389
Hpy8I GTNNAC 1 cut(s) 323
HpyAV CCTTC 1 cut(s) 392
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 2 cut(s) 191, 248
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 29, 288
Hsp92II CATG 2 cut(s) 59, 121
HspAI GCGC 1 cut(s) 173
KflI GGGWCCC 1 cut(s) 438
KpnI GGTACC 1 cut(s) 231
Kzo9I GATC 3 cut(s) 25, 301, 349
LguI GCTCTTC 1 cut(s) 45
LmnI GCTCC 1 cut(s) 430
LpnPI CCDG 9 cut(s) 99, 210, 216, 246, 374, 394, 402, 420, 447
Lsp1109I GCAGC 1 cut(s) 269
MaeI CTAG 2 cut(s) 60, 341
MalI GATC 3 cut(s) 27, 303, 351
MboI GATC 3 cut(s) 25, 301, 349
MboII GAAGA 4 cut(s) 35, 62, 71, 208
MfeI CAATTG 1 cut(s) 192
MflI RGATCY 2 cut(s) 25, 301
MhlI GDGCHC 2 cut(s) 43, 289
MluCI AATT 3 cut(s) 192, 237, 393
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 6 cut(s) 30, 135, 154, 265, 292, 452
MseI TTAA 1 cut(s) 460
MslI CAYNNNNRTG 1 cut(s) 310
MspI CCGG 1 cut(s) 361
MspR9I CCNGG 1 cut(s) 435
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeII GATC 3 cut(s) 25, 301, 349
NlaIII CATG 2 cut(s) 59, 121
NlaIV GGNNCC 4 cut(s) 229, 303, 439, 440
NsbI TGCGCA 1 cut(s) 174
NspI RCATGY 2 cut(s) 59, 121
PaeI GCATGC 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 45
PfeI GAWTC 1 cut(s) 76
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PpuMI RGGWCCY 1 cut(s) 438
Psp5II RGGWCCY 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 433
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 4 cut(s) 229, 303, 439, 440
PspPI GGNCC 1 cut(s) 438
PspPPI RGGWCCY 1 cut(s) 438
PsuI RGATCY 2 cut(s) 25, 301
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 1 cut(s) 460
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 3 cut(s) 25, 301, 349
Sau96I GGNCC 1 cut(s) 438
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 2 cut(s) 43, 289
SetI ASST 8 cut(s) 71, 111, 188, 215, 262, 322, 403, 413
SinI GGWCC 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 310
SphI GCATGC 1 cut(s) 59
Sse9I AATT 3 cut(s) 192, 237, 393
SsiI CCGC 2 cut(s) 283, 331
SspMI CTAG 2 cut(s) 60, 341
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 2 cut(s) 103, 262
TasI AATT 3 cut(s) 192, 237, 393
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 1 cut(s) 460
Tru9I TTAA 1 cut(s) 460
TseI GCWGC 1 cut(s) 257
TspGWI ACGGA 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 438
XagI CCTNNNNNAGG 1 cut(s) 432
XceI RCATGY 2 cut(s) 59, 121
XspI CTAG 2 cut(s) 60, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.