MD05G1017500.v1.1

DUF218 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011939
Physical Location & Seq
Reverse (-)
3060804 .. 3064938
4135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1017500.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGAGCAATTATCCGTTTCGGCCCGGCAGTCCCAAGTCCTTTGTAGCCTACCCGAGTGGTGAGTTTGATTTAGAATCTGGAACATTTAGAAAACCCCGAAAGCATAGGAGTTCATCATCGCATCCCATCAAAATGATTAGGTCTATCGGAAACCGAATTCAGTATTACTGCAAGCTGCATCCCCTTTTAGTTTTCGTCATCTCCTTGTCAGTTGGGGTCTCAATTCTCATACTTTTGTCTCTGTATGAGAGAGGGTACCAAATGACGAATAACTACCAAAACTATGACGTGAGTTCGTCTAGTTATCCGTTACCGAATCTCCAGAATCTAGTCATGGTTGCCGGGCATTCAGTTTATACTAGTAGTAGCTGTGGAAAAGTTGATAAGGAGGATTCTTGGTTTTTGGAGTCTTACCCGAAGAATCCAGGTCAGGCTGCTACTTTCGTGGCTCATATACAAGAGGGAATTGGAGTCGCAGCCAAAGACAATAGAGCTCTCCTGCTGTTTAGTGGTGGAGAGACTCGAAGAGAGGCCGGTCCTCGCAGTGAAGCACAGAGTTACTGGGCAGTTGCTGAATCAAAAGGATGGTTTGGCCAAGAAGATTTGAGGTCGAGGACACTCACAGAAGAGCATGCAAGGGACAGTTTTGAGAATCTTCTCTTCAGTGTGTGCCGATTCCGAGAACTTACTGGCACATATCCTCAAAATATAACTGTCGTAAGTTATGATTTCAAGGAGCAGAGATTTGTGCATCTACACCGCGCTGCAATTGGCTTTCCTGAGTCAAGGTTCTTCTATGTGGGTACCCCAGCAATTTCCACTGCAAAAGTAGCAGCCCAGAAAGGAGAAGCAATTGTGCGAGCTCAATTCCAAGAAGATCCATATGGATGTCGAGGTTCACTTTACCGTAAAAAACTAGGGCGTGATCCATTTCACAGGTCTATTCCCTATCCAAATGGGTGTCCTGAAATTGAAGGTCTTTTCAGATATTGTGGGTCAGCTCCTTATCCAGGGTCCCTTCCTTGGCCTAAAGAGCTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF218

Protein Analysis

348

Amino Acids

39.14

Weight (kDa)

9.15

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21370 AT1G21370
fragaria_vesca FvH4_2g14460 FvH4_2g14460
malus_domestica MD05G1017500.v1.1 MD10G1017500.v1.1
prunus_persica Prupe.8G021400_v2.0.a1
pyrus_communis pycom05g00980 pycom10g01120
rosa_chinensis RchiOBHm_Chr6g0276791
rosa_laevigata RLG00000013361
rosa_multiflora Rmu_co8479231.1_g000001
rosa_roxburghii Rroxscaffold_7G00191940
rosa_rugosa Rorug06G0104500
rosa_samantha Rh6AG214400 Rh6BG219200 Rh6CG221900 Rh6DG211900
rosa_wichuraiana Rw6G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 255, 803
AccB1I GGYRCC 2 cut(s) 255, 803
AccII CGCG 1 cut(s) 762
AciI CCGC 1 cut(s) 760
AclWI GGATC 2 cut(s) 872, 920
AcoI YGGCCR 1 cut(s) 592
AcsI RAATTY 1 cut(s) 156
AcuI CTGAAG 1 cut(s) 646
AfaI GTAC 2 cut(s) 257, 805
AfiI CCNNNNNNNGG 2 cut(s) 1010, 1023
AgsI TTSAA 2 cut(s) 733, 974
AhlI ACTAGT 1 cut(s) 359
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 2 cut(s) 424, 1009
AluBI AGCT 6 cut(s) 175, 369, 494, 863, 1001, 1036
AluI AGCT 6 cut(s) 175, 369, 494, 863, 1001, 1036
Alw21I GWGCWC 2 cut(s) 496, 865
Alw26I GTCTC 3 cut(s) 223, 243, 512
AlwI GGATC 2 cut(s) 872, 920
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 4 cut(s) 20, 531, 592, 1025
ApeKI GCWGC 5 cut(s) 175, 434, 476, 764, 833
ApoI RAATTY 1 cut(s) 156
Asp718I GGTACC 2 cut(s) 255, 803
AspLEI GCGC 1 cut(s) 764
AspS9I GGNCC 3 cut(s) 21, 536, 1014
AsuC2I CCSGG 2 cut(s) 24, 343
AsuHPI GGTGA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 2 cut(s) 536, 1014
BalI TGGCCA 1 cut(s) 594
BanI GGYRCC 2 cut(s) 255, 803
BanII GRGCYC 2 cut(s) 496, 865
Bbv12I GWGCWC 2 cut(s) 496, 865
BbvI GCAGC 5 cut(s) 162, 421, 488, 751, 845
BccI CCATC 2 cut(s) 134, 579
BciT130I CCWGG 2 cut(s) 426, 1011
BcnI CCSGG 2 cut(s) 24, 343
BcoDI GTCTC 3 cut(s) 223, 243, 512
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 4 cut(s) 300, 329, 360, 917
BisI GCNGC 5 cut(s) 176, 435, 477, 765, 834
BlsI GCNGC 5 cut(s) 177, 436, 478, 766, 835
Bme1390I CCNGG 4 cut(s) 24, 343, 426, 1011
Bme18I GGWCC 2 cut(s) 536, 1014
BmeT110I CYCGRG 1 cut(s) 52
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 3 cut(s) 21, 536, 1014
BmiI GGNNCC 4 cut(s) 257, 805, 1015, 1016
BmrFI CCNGG 4 cut(s) 24, 343, 426, 1011
BmrI ACTGGG 1 cut(s) 571
BmsI GCATC 3 cut(s) 130, 187, 760
BmuI ACTGGG 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 2 cut(s) 24, 343
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 1010, 1022
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bsc4I CCNNNNNNNGG 2 cut(s) 1010, 1023
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 2 cut(s) 566, 694
BseBI CCWGG 2 cut(s) 426, 1011
BseDI CCNNGG 2 cut(s) 1010, 1022
BseGI GGATG 4 cut(s) 121, 178, 590, 893
BseLI CCNNNNNNNGG 2 cut(s) 1010, 1023
BseMII CTCAG 1 cut(s) 771
BseNI ACTGG 2 cut(s) 566, 694
BseXI GCAGC 5 cut(s) 162, 421, 488, 751, 845
BseYI CCCAGC 1 cut(s) 808
Bsh1236I CGCG 1 cut(s) 762
BshFI GGCC 4 cut(s) 22, 533, 594, 1027
BshNI GGYRCC 2 cut(s) 255, 803
BsiHKAI GWGCWC 2 cut(s) 496, 865
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 3 cut(s) 24, 342, 534
BslFI GGGAC 3 cut(s) 15, 653, 1000
BslI CCNNNNNNNGG 2 cut(s) 1010, 1023
BsmAI GTCTC 3 cut(s) 223, 243, 512
BsmFI GGGAC 3 cut(s) 15, 653, 1000
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 4 cut(s) 22, 533, 594, 1027
Bso31I GGTCTC 1 cut(s) 223
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 2 cut(s) 496, 865
Bsp143I GATC 2 cut(s) 877, 925
BspACI CCGC 1 cut(s) 760
BspANI GGCC 4 cut(s) 22, 533, 594, 1027
BspCNI CTCAG 1 cut(s) 772
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 4 cut(s) 257, 805, 1015, 1016
BspPI GGATC 2 cut(s) 872, 920
BspQI GCTCTTC 1 cut(s) 621
BspT107I GGYRCC 2 cut(s) 255, 803
BspTNI GGTCTC 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 2 cut(s) 566, 694
BssAI RCCGGY 1 cut(s) 533
BssECI CCNNGG 2 cut(s) 1010, 1022
BssMI GATC 2 cut(s) 877, 925
BssT1I CCWWGG 1 cut(s) 1022
Bst2UI CCWGG 2 cut(s) 426, 1011
Bst4CI ACNGT 3 cut(s) 644, 715, 908
Bst6I CTCTTC 3 cut(s) 520, 621, 665
BstC8I GCNNGC 3 cut(s) 173, 633, 861
BstDEI CTNAG 1 cut(s) 780
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstF5I GGATG 4 cut(s) 121, 178, 590, 893
BstFNI CGCG 1 cut(s) 762
BstHHI GCGC 1 cut(s) 764
BstKTI GATC 2 cut(s) 880, 928
BstMAI GTCTC 3 cut(s) 223, 243, 512
BstMBI GATC 2 cut(s) 877, 925
BstMWI GCNNNNNNNGC 2 cut(s) 830, 1033
BstNI CCWGG 2 cut(s) 426, 1011
BstNSI RCATGY 1 cut(s) 635
BstSCI CCNGG 4 cut(s) 22, 341, 424, 1009
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 5 cut(s) 162, 421, 488, 751, 845
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
BsuRI GGCC 4 cut(s) 22, 533, 594, 1027
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 4 cut(s) 121, 178, 590, 893
BtsI GCAGTG 2 cut(s) 550, 819
BtsIMutI CAGTG 3 cut(s) 550, 670, 819
Cac8I GCNNGC 3 cut(s) 173, 633, 861
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 1 cut(s) 764
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 3 cut(s) 21, 536, 1014
Csp6I GTAC 2 cut(s) 256, 804
CviAII CATG 2 cut(s) 334, 632
CviQI GTAC 2 cut(s) 256, 804
DdeI CTNAG 1 cut(s) 780
DpnI GATC 2 cut(s) 879, 927
DpnII GATC 2 cut(s) 877, 925
EaeI YGGCCR 1 cut(s) 592
Eam1104I CTCTTC 3 cut(s) 520, 621, 665
EarI CTCTTC 3 cut(s) 520, 621, 665
Ecl136II GAGCTC 2 cut(s) 494, 863
Eco130I CCWWGG 1 cut(s) 1022
Eco24I GRGCYC 2 cut(s) 496, 865
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 536, 1014
Eco53kI GAGCTC 2 cut(s) 494, 863
Eco57I CTGAAG 1 cut(s) 646
Eco88I CYCGRG 1 cut(s) 52
EcoICRI GAGCTC 2 cut(s) 494, 863
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoO109I RGGNCCY 1 cut(s) 1014
EcoRI GAATTC 1 cut(s) 156
EcoRII CCWGG 2 cut(s) 424, 1009
EcoT14I CCWWGG 1 cut(s) 1022
EcoT38I GRGCYC 2 cut(s) 496, 865
ErhI CCWWGG 1 cut(s) 1022
FaeI CATG 2 cut(s) 337, 635
FaqI GGGAC 3 cut(s) 15, 653, 1000
FatI CATG 2 cut(s) 333, 631
FauNDI CATATG 1 cut(s) 883
Fnu4HI GCNGC 5 cut(s) 176, 435, 477, 765, 834
FokI GGATG 4 cut(s) 108, 165, 597, 900
FriOI GRGCYC 2 cut(s) 496, 865
Fsp4HI GCNGC 5 cut(s) 176, 435, 477, 765, 834
FspBI CTAG 4 cut(s) 300, 329, 360, 917
GlaI GCGC 1 cut(s) 763
GluI GCNGC 5 cut(s) 176, 435, 477, 765, 834
GsaI CCCAGC 1 cut(s) 812
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 4 cut(s) 22, 533, 594, 1027
HapII CCGG 3 cut(s) 24, 342, 534
HhaI GCGC 1 cut(s) 764
Hin1II CATG 2 cut(s) 337, 635
Hin6I GCGC 1 cut(s) 762
HinP1I GCGC 1 cut(s) 762
HpaII CCGG 3 cut(s) 24, 342, 534
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 899
Hpy188I TCNGA 3 cut(s) 149, 680, 986
Hpy188III TCNNGA 4 cut(s) 78, 322, 779, 965
Hpy8I GTNNAC 1 cut(s) 899
HpyAV CCTTC 2 cut(s) 968, 1028
HpyCH4III ACNGT 3 cut(s) 644, 715, 908
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 6 cut(s) 171, 178, 635, 751, 767, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 830, 1033
HpyF3I CTNAG 1 cut(s) 780
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 2 cut(s) 337, 635
HspAI GCGC 1 cut(s) 762
KflI GGGWCCC 1 cut(s) 1014
KpnI GGTACC 2 cut(s) 259, 807
Kzo9I GATC 2 cut(s) 877, 925
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 2 cut(s) 736, 1006
Lsp1109I GCAGC 5 cut(s) 162, 421, 488, 751, 845
LweI GCATC 3 cut(s) 130, 187, 760
MaeI CTAG 4 cut(s) 300, 329, 360, 917
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 2 cut(s) 309, 557
MalI GATC 2 cut(s) 879, 927
MboI GATC 2 cut(s) 877, 925
MboII GAAGA 8 cut(s) 430, 537, 611, 638, 647, 652, 784, 887
MfeI CAATTG 2 cut(s) 768, 852
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 2 cut(s) 496, 865
MlsI TGGCCA 1 cut(s) 594
MluNI TGGCCA 1 cut(s) 594
MlyI GAGTC 4 cut(s) 416, 480, 514, 791
MnlI CCTC 9 cut(s) 245, 382, 454, 523, 549, 600, 606, 711, 887
Mox20I TGGCCA 1 cut(s) 594
MscI TGGCCA 1 cut(s) 594
MseI TTAA 1 cut(s) 1042
MslI CAYNNNNRTG 2 cut(s) 131, 886
Msp20I TGGCCA 1 cut(s) 594
MspI CCGG 3 cut(s) 24, 342, 534
MspR9I CCNGG 4 cut(s) 24, 343, 426, 1011
MunI CAATTG 2 cut(s) 768, 852
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 2 cut(s) 426, 1011
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 2 cut(s) 830, 1033
NciI CCSGG 2 cut(s) 24, 343
NdeI CATATG 1 cut(s) 883
NdeII GATC 2 cut(s) 877, 925
NlaIII CATG 2 cut(s) 337, 635
NlaIV GGNNCC 4 cut(s) 257, 805, 1015, 1016
NspI RCATGY 1 cut(s) 635
PaeI GCATGC 1 cut(s) 635
PciSI GCTCTTC 1 cut(s) 621
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 8 cut(s) 74, 316, 325, 392, 421, 575, 652, 675
PkrI GCNGC 5 cut(s) 177, 436, 478, 766, 835
PleI GAGTC 4 cut(s) 415, 479, 514, 790
PpsI GAGTC 4 cut(s) 415, 479, 514, 790
PpuMI RGGWCCY 1 cut(s) 1014
Psp124BI GAGCTC 2 cut(s) 496, 865
Psp5II RGGWCCY 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 424, 1009
PspFI CCCAGC 1 cut(s) 808
PspGI CCWGG 2 cut(s) 424, 1009
PspN4I GGNNCC 4 cut(s) 257, 805, 1015, 1016
PspPI GGNCC 3 cut(s) 21, 536, 1014
PspPPI RGGWCCY 1 cut(s) 1014
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 1 cut(s) 877
RsaI GTAC 2 cut(s) 257, 805
RsaNI GTAC 2 cut(s) 256, 804
RseI CAYNNNNRTG 2 cut(s) 131, 886
SacI GAGCTC 2 cut(s) 496, 865
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 1 cut(s) 1042
SatI GCNGC 5 cut(s) 176, 435, 477, 765, 834
Sau3AI GATC 2 cut(s) 877, 925
Sau96I GGNCC 3 cut(s) 21, 536, 1014
SchI GAGTC 4 cut(s) 416, 480, 514, 791
ScrFI CCNGG 4 cut(s) 24, 343, 426, 1011
SduI GDGCHC 2 cut(s) 496, 865
SfaNI GCATC 3 cut(s) 130, 187, 760
SinI GGWCC 2 cut(s) 536, 1014
SmiMI CAYNNNNRTG 2 cut(s) 131, 886
SpeI ACTAGT 1 cut(s) 359
SphI GCATGC 1 cut(s) 635
SsiI CCGC 1 cut(s) 760
SspMI CTAG 4 cut(s) 300, 329, 360, 917
SstI GAGCTC 2 cut(s) 496, 865
StyD4I CCNGG 4 cut(s) 22, 341, 424, 1009
StyI CCWWGG 1 cut(s) 1022
TaaI ACNGT 3 cut(s) 644, 715, 908
TaiI ACGT 1 cut(s) 291
TaqI TCGA 3 cut(s) 523, 611, 892
TfiI GAWTC 8 cut(s) 74, 316, 325, 392, 421, 575, 652, 675
Tru1I TTAA 1 cut(s) 1042
Tru9I TTAA 1 cut(s) 1042
TscAI CASTG 3 cut(s) 550, 670, 826
TseI GCWGC 5 cut(s) 175, 434, 476, 764, 833
TspDTI ATGAA 1 cut(s) 102
TspGWI ACGGA 1 cut(s) 297
TspRI CASTG 3 cut(s) 550, 670, 826
VpaK11BI GGWCC 2 cut(s) 536, 1014
XagI CCTNNNNNAGG 1 cut(s) 1008
XapI RAATTY 1 cut(s) 156
XceI RCATGY 1 cut(s) 635
XspI CTAG 4 cut(s) 300, 329, 360, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.