Rh6AG214400

DUF218 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0011939
Physical Location & Seq
Forward (+)
38340266 .. 38344501
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG214400.1

Sequence Viewer

Length: 780 bp
ATGAGCAATAATCCATATGGGTCTGGCAATCCCAAGTCCCCCAAGTCCTTTGTTGCATACCCAAGTGGTGAGTTTGATTTAGAATCTGGAACCATCCGAAGAACCCGAAAACATAGAAAATCGAAACTCCATCCCATCAAAATGATTAGGTCTGTCGGGAACCGCATGAAGTACTACTGCAAGCTGCACCCTCTGTTGGTTTTTATGATCACTTTGTCAGTTGGGGTCACAATTCTTGTACTGTTGCCTGTCTATGAGAACCATTTTCAAATGAATAACTATCGAAAATTTGTCATGGATTTCGATAGTTATCCTTTACCGAATCTCAAGAATCTTGTCATGGTTGCTGGGCATTCAGTTTATACTAGTAATAGCTGCGGAAAAGTTGATAAGGAGGATTCTTGGTTTTTGGAGTCTTATCAGAAGAATCCCGGGCAAGCTTCTACTTTTGTGGCCCATATACAAGAGGGAATTGAAATTGCGGCCAAAGACAATGCAGCTCTGCTTCTTTTTAGTGGTGGAGAAACTCGGAGAGATGCTGGTCCTCGGAGTGAGGCACAGAGTTATTGGGCAGTTGCTGAATCAAAAAAATGGTTTGGCAAAGAAGATCTGAGACGGAGGGCACTCACTGAAGAGCATGCTAGGGATAGCTTTGAGAATCTTCTCTTTAGTGTTTGCCGATTTCGAGAGCTTACTGGCATGTATCCTCATAATATAACTGTAAGTCGATGTTGTTCAACCTTTGGTTATGCTTATTTAGAGCTAAGCACAGGTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF218

Protein Analysis

259

Amino Acids

29.52

Weight (kDa)

9.27

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21370 AT1G21370
fragaria_vesca FvH4_2g14460 FvH4_2g14460
malus_domestica MD05G1017500.v1.1 MD10G1017500.v1.1
prunus_persica Prupe.8G021400_v2.0.a1
pyrus_communis pycom05g00980 pycom10g01120
rosa_chinensis RchiOBHm_Chr6g0276791
rosa_laevigata RLG00000013361
rosa_multiflora Rmu_co8479231.1_g000001
rosa_roxburghii Rroxscaffold_7G00191940
rosa_rugosa Rorug06G0104500
rosa_samantha Rh6AG214400 Rh6BG219200 Rh6CG221900 Rh6DG211900
rosa_wichuraiana Rw6G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 163, 378, 482
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 2 cut(s) 173, 240
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 3 cut(s) 269, 476, 738
AhlI ACTAGT 1 cut(s) 365
AluBI AGCT 7 cut(s) 184, 375, 440, 500, 651, 691, 763
AluI AGCT 7 cut(s) 184, 375, 440, 500, 651, 691, 763
Alw26I GTCTC 1 cut(s) 607
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 2 cut(s) 453, 483
ApeKI GCWGC 3 cut(s) 184, 375, 497
ApoI RAATTY 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 454, 542
AsuC2I CCSGG 2 cut(s) 432, 433
AsuHPI GGTGA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 625
BbvI GCAGC 3 cut(s) 171, 362, 509
BccI CCATC 3 cut(s) 101, 138, 143
BciVI GTATCC 1 cut(s) 714
BclI TGATCA 1 cut(s) 207
BcnI CCSGG 2 cut(s) 432, 433
BcoDI GTCTC 1 cut(s) 607
BcuI ACTAGT 1 cut(s) 365
BfaI CTAG 2 cut(s) 366, 642
BfuI GTATCC 1 cut(s) 714
BglII AGATCT 1 cut(s) 607
BisI GCNGC 4 cut(s) 185, 376, 483, 498
BlpI GCTNAGC 1 cut(s) 764
BlsI GCNGC 4 cut(s) 186, 377, 484, 499
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 2 cut(s) 432, 433
Bme18I GGWCC 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 454, 542
BmiI GGNNCC 2 cut(s) 91, 161
BmrFI CCNGG 2 cut(s) 432, 433
BmsI GCATC 1 cut(s) 526
Bpu1102I GCTNAGC 1 cut(s) 764
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 2 cut(s) 432, 433
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 431, 545
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 700
Bse8I GATNNNNATC 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 431, 545
BseGI GGATG 2 cut(s) 93, 130
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 1 cut(s) 700
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 3 cut(s) 171, 362, 509
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 170
BshFI GGCC 2 cut(s) 455, 485
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 1 cut(s) 432
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 1 cut(s) 607
BsmBI CGTCTC 1 cut(s) 607
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 2 cut(s) 455, 485
BsoBI CYCGRG 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 2 cut(s) 207, 607
Bsp1720I GCTNAGC 1 cut(s) 764
BspACI CCGC 3 cut(s) 163, 378, 482
BspANI GGCC 2 cut(s) 455, 485
BspCNI CTCAG 1 cut(s) 603
BspLI GGNNCC 2 cut(s) 91, 161
BspQI GCTCTTC 1 cut(s) 627
BsrI ACTGG 1 cut(s) 700
BssECI CCNNGG 2 cut(s) 431, 545
BssMI GATC 2 cut(s) 207, 607
Bst4CI ACNGT 2 cut(s) 243, 721
Bst6I CTCTTC 1 cut(s) 627
BstC8I GCNNGC 3 cut(s) 182, 438, 639
BstDEI CTNAG 2 cut(s) 611, 764
BstF5I GGATG 2 cut(s) 93, 130
BstKTI GATC 2 cut(s) 210, 610
BstMAI GTCTC 1 cut(s) 607
BstMBI GATC 2 cut(s) 207, 607
BstNSI RCATGY 2 cut(s) 641, 703
BstSCI CCNGG 2 cut(s) 430, 431
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 3 cut(s) 171, 362, 509
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
BsuI GTATCC 1 cut(s) 714
BsuRI GGCC 2 cut(s) 455, 485
BtsCI GGATG 2 cut(s) 93, 130
BtsIMutI CAGTG 1 cut(s) 627
Cac8I GCNNGC 3 cut(s) 182, 438, 639
CaiI CAGNNNCTG 1 cut(s) 578
Cfr13I GGNCC 2 cut(s) 454, 542
Cfr9I CCCGGG 1 cut(s) 431
Csp6I GTAC 2 cut(s) 172, 239
CviAII CATG 5 cut(s) 166, 295, 340, 638, 700
CviJI RGCY 9 cut(s) 184, 375, 440, 455, 485, 500, 651, 691, 763
CviKI_1 RGCY 9 cut(s) 184, 375, 440, 455, 485, 500, 651, 691, 763
CviQI GTAC 2 cut(s) 172, 239
DdeI CTNAG 2 cut(s) 611, 764
DpnI GATC 2 cut(s) 209, 609
DpnII GATC 2 cut(s) 207, 607
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
Eco47I GGWCC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 651
Eco88I CYCGRG 1 cut(s) 431
Esp3I CGTCTC 1 cut(s) 607
FaeI CATG 5 cut(s) 169, 298, 343, 641, 703
FaqI GGGAC 1 cut(s) 22
FatI CATG 5 cut(s) 165, 294, 339, 637, 699
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 185, 376, 483, 498
FokI GGATG 2 cut(s) 80, 117
Fsp4HI GCNGC 4 cut(s) 185, 376, 483, 498
FspBI CTAG 2 cut(s) 366, 642
GluI GCNGC 4 cut(s) 185, 376, 483, 498
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 2 cut(s) 455, 485
HapII CCGG 1 cut(s) 432
Hin1II CATG 5 cut(s) 169, 298, 343, 641, 703
HindIII AAGCTT 1 cut(s) 438
HinfI GANTC 8 cut(s) 83, 322, 331, 398, 413, 427, 581, 658
HpaII CCGG 1 cut(s) 432
HphI GGTGA 1 cut(s) 80
Hpy188I TCNGA 5 cut(s) 98, 423, 531, 549, 612
Hpy188III TCNNGA 4 cut(s) 87, 157, 328, 686
HpyCH4III ACNGT 2 cut(s) 243, 721
HpyCH4V TGCA 4 cut(s) 56, 180, 187, 497
HpyF3I CTNAG 2 cut(s) 611, 764
Hsp92II CATG 5 cut(s) 169, 298, 343, 641, 703
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 2 cut(s) 207, 607
LguI GCTCTTC 1 cut(s) 627
LpnPI CCDG 8 cut(s) 9, 72, 261, 333, 445, 525, 681, 756
Lsp1109I GCAGC 3 cut(s) 171, 362, 509
LweI GCATC 1 cut(s) 526
MaeI CTAG 2 cut(s) 366, 642
MaeIII GTNAC 1 cut(s) 226
MalI GATC 2 cut(s) 209, 609
MboI GATC 2 cut(s) 207, 607
MboII GAAGA 5 cut(s) 111, 436, 617, 644, 653
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 4 cut(s) 231, 287, 471, 477
MlyI GAGTC 1 cut(s) 422
MnlI CCTC 7 cut(s) 201, 388, 460, 547, 555, 612, 717
MslI CAYNNNNRTG 2 cut(s) 140, 773
MspI CCGG 1 cut(s) 432
MspR9I CCNGG 2 cut(s) 432, 433
Mva1269I GAATGC 1 cut(s) 352
NciI CCSGG 2 cut(s) 432, 433
NdeI CATATG 1 cut(s) 16
NdeII GATC 2 cut(s) 207, 607
NlaIII CATG 5 cut(s) 169, 298, 343, 641, 703
NlaIV GGNNCC 2 cut(s) 91, 161
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 2 cut(s) 641, 703
PaeI GCATGC 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 627
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 7 cut(s) 83, 322, 331, 398, 427, 581, 658
PkrI GCNGC 4 cut(s) 186, 377, 484, 499
PleI GAGTC 1 cut(s) 421
PpsI GAGTC 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 347
PspN4I GGNNCC 2 cut(s) 91, 161
PspPI GGNCC 2 cut(s) 454, 542
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 607
RsaI GTAC 2 cut(s) 173, 240
RsaNI GTAC 2 cut(s) 172, 239
RseI CAYNNNNRTG 2 cut(s) 140, 773
SapI GCTCTTC 1 cut(s) 627
SatI GCNGC 4 cut(s) 185, 376, 483, 498
Sau3AI GATC 2 cut(s) 207, 607
Sau96I GGNCC 2 cut(s) 454, 542
ScaI AGTACT 1 cut(s) 173
SchI GAGTC 1 cut(s) 422
ScrFI CCNGG 2 cut(s) 432, 433
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 1 cut(s) 526
SinI GGWCC 1 cut(s) 542
SmaI CCCGGG 1 cut(s) 433
SmiMI CAYNNNNRTG 2 cut(s) 140, 773
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SpeI ACTAGT 1 cut(s) 365
SphI GCATGC 1 cut(s) 641
Sse9I AATT 4 cut(s) 231, 287, 471, 477
SsiI CCGC 3 cut(s) 163, 378, 482
SspMI CTAG 2 cut(s) 366, 642
StyD4I CCNGG 2 cut(s) 430, 431
TaaI ACNGT 2 cut(s) 243, 721
TaqI TCGA 5 cut(s) 122, 283, 303, 685, 727
TasI AATT 4 cut(s) 231, 287, 471, 477
TatI WGTACW 2 cut(s) 171, 238
TauI GCSGC 1 cut(s) 485
TfiI GAWTC 7 cut(s) 83, 322, 331, 398, 427, 581, 658
TscAI CASTG 1 cut(s) 634
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 3 cut(s) 184, 375, 497
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 2 cut(s) 182, 287
TspGWI ACGGA 1 cut(s) 631
TspMI CCCGGG 1 cut(s) 431
TspRI CASTG 1 cut(s) 634
VpaK11BI GGWCC 1 cut(s) 542
XapI RAATTY 1 cut(s) 287
XceI RCATGY 2 cut(s) 641, 703
XmaI CCCGGG 1 cut(s) 431
XspI CTAG 2 cut(s) 366, 642
ZrmI AGTACT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.