MD10G1017500.v1.1

DUF218 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011939
Physical Location & Seq
Reverse (-)
2230226 .. 2233860
3635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1017500.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGAGCAATAATCCGTTTCGGCCCGGCAGTCCTAAGTCCTTCGTAGCCTACCCGAGTGGTGAGTTTGATTTAGAATCTGGAACATTTAGAAAACCCCGAAAGCATAGGAGGTCACCATTGCTTCCCATCAAAATGATTAGGTATATCGGAAACCGAATGCAGTATTATTGCAAGCTGCGTCCCCTTTTAGTTTTCATCCTCTCCTTGTCAGTTGGGGTCTCAATTCTCATACTTTTGTCTCTGTATGAGAGCCGGTATCAAATGACGAACAACTACCAAAAGTATGACGTGGGTTCTTCTAGTTATCCGCTCCCGAATCTCCAGAATCTAGTCATGGTTGCCGGGCATTCAGTTTATACTAGTAGTAGCTGTGGAAAAGTTGATAAGGAGGATTCTTGGTTTTTGGAGTCCTACCAAAAGAATCCGGGTCAGGCTGCTACTTTCGTGGCTCATATACAAGAAGGAATTGAAGTCGCGGCCAAAGACAATAGAGCTCTCCTTCTGTTTAGTGGTGGAGAGACTCGAAGAGATGCCGGTCCTCGCAGTGAGGCACAGAGTTACTGGGCAGTTGCTGAATCAAAAGGATGGTTTGGCCAAGAAGATTTGCGATCGAGGACACTCACAGAAGAGCATGCAAGGGACAGTTTTGAGAATCTTCTCTTCAGTGTGTGCCGGTTCCGAGAACTTACTGGCAAATATCCTCAAAATATAACTGTTGTAAGTTATGATTTCAAGGAGGAGAGATTCGCGCATCTACACCGGGCTGCAATTGGCTTTCCCGAGTCAAGGTTCTTCTACGTGGGCACCCCAGCAATTTCTACTGCAAAAGAAGCAGCCCGGAAAGGAGAAGCAGTCGTGCGGACTCAATTCCAAGAAGATCCATATGGATGTCGAGGTTCACTTTACCGTAAAAAACTAGGGCGCGATCCGTTTCACCGGTCGGTTCCCTATCCAAATGGGTGTCCTGAAATTGAAGGTCTTTTCAGATATTGTGGGTCAGCTCCTTATCCAGGGTCCCTTCCGTGGCCTAAAGAGCAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF218

Protein Analysis

348

Amino Acids

39.46

Weight (kDa)

9.28

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011939)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21370 AT1G21370
fragaria_vesca FvH4_2g14460 FvH4_2g14460
malus_domestica MD05G1017500.v1.1 MD10G1017500.v1.1
prunus_persica Prupe.8G021400_v2.0.a1
pyrus_communis pycom05g00980 pycom10g01120
rosa_chinensis RchiOBHm_Chr6g0276791
rosa_laevigata RLG00000013361
rosa_multiflora Rmu_co8479231.1_g000001
rosa_roxburghii Rroxscaffold_7G00191940
rosa_rugosa Rorug06G0104500
rosa_samantha Rh6AG214400 Rh6BG219200 Rh6CG221900 Rh6DG211900
rosa_wichuraiana Rw6G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 803
AccBSI CCGCTC 1 cut(s) 310
AccII CGCG 3 cut(s) 476, 749, 924
AciI CCGC 3 cut(s) 308, 476, 859
AclWI GGATC 2 cut(s) 872, 920
AcoI YGGCCR 2 cut(s) 477, 592
AcuI CTGAAG 1 cut(s) 646
AfiI CCNNNNNNNGG 3 cut(s) 786, 1010, 1023
AgeI ACCGGT 1 cut(s) 936
AgsI TTSAA 3 cut(s) 470, 733, 974
AhlI ACTAGT 1 cut(s) 359
AjiI CACGTC 1 cut(s) 289
AjnI CCWGG 1 cut(s) 1009
AluBI AGCT 4 cut(s) 175, 369, 494, 1001
AluI AGCT 4 cut(s) 175, 369, 494, 1001
Alw21I GWGCWC 1 cut(s) 496
Alw26I GTCTC 3 cut(s) 223, 243, 512
AlwI GGATC 2 cut(s) 872, 920
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 2 cut(s) 52, 779
AoxI GGCC 4 cut(s) 20, 477, 592, 1025
ApeKI GCWGC 4 cut(s) 175, 434, 764, 833
AsiGI ACCGGT 1 cut(s) 936
AspLEI GCGC 2 cut(s) 751, 924
AspS9I GGNCC 3 cut(s) 21, 536, 1014
AsuC2I CCSGG 5 cut(s) 24, 343, 426, 761, 838
AsuHPI GGTGA 3 cut(s) 71, 105, 926
AvaI CYCGRG 2 cut(s) 52, 779
AvaII GGWCC 2 cut(s) 536, 1014
BaeGI GKGCMC 1 cut(s) 806
BalI TGGCCA 1 cut(s) 594
BanI GGYRCC 1 cut(s) 803
BanII GRGCYC 1 cut(s) 496
Bbv12I GWGCWC 1 cut(s) 496
BbvI GCAGC 4 cut(s) 162, 421, 751, 845
BccI CCATC 2 cut(s) 134, 579
BciT130I CCWGG 1 cut(s) 1011
BcnI CCSGG 5 cut(s) 24, 343, 426, 761, 838
BcoDI GTCTC 3 cut(s) 223, 243, 512
BcuI ACTAGT 1 cut(s) 359
BfaI CTAG 4 cut(s) 300, 329, 360, 917
BisI GCNGC 5 cut(s) 176, 435, 477, 765, 834
BlsI GCNGC 5 cut(s) 177, 436, 478, 766, 835
Bme1390I CCNGG 6 cut(s) 24, 343, 426, 761, 838, 1011
Bme18I GGWCC 2 cut(s) 536, 1014
BmeT110I CYCGRG 2 cut(s) 52, 779
BmgBI CACGTC 1 cut(s) 289
BmgT120I GGNCC 3 cut(s) 21, 536, 1014
BmiI GGNNCC 5 cut(s) 677, 805, 945, 1015, 1016
BmrFI CCNGG 6 cut(s) 24, 343, 426, 761, 838, 1011
BmrI ACTGGG 1 cut(s) 571
BmsI GCATC 2 cut(s) 520, 760
BmuI ACTGGG 1 cut(s) 571
BpmI CTGGAG 1 cut(s) 305
BpuMI CCSGG 5 cut(s) 24, 343, 426, 761, 838
BsaAI YACGTR 1 cut(s) 799
BsaI GGTCTC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 1010, 1022
BsaWI WCCGGW 1 cut(s) 936
BsaXI ACNNNNNCTCC 2 cut(s) 837, 867
Bsc4I CCNNNNNNNGG 3 cut(s) 786, 1010, 1023
Bse118I RCCGGY 4 cut(s) 252, 533, 672, 936
Bse1I ACTGG 2 cut(s) 566, 694
Bse3DI GCAATG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 1011
BseDI CCNNGG 2 cut(s) 1010, 1022
BseGI GGATG 3 cut(s) 195, 590, 893
BseLI CCNNNNNNNGG 3 cut(s) 786, 1010, 1023
BseMI GCAATG 1 cut(s) 116
BseNI ACTGG 2 cut(s) 566, 694
BseRI GAGGAG 1 cut(s) 752
BseSI GKGCMC 1 cut(s) 806
BseXI GCAGC 4 cut(s) 162, 421, 751, 845
BseYI CCCAGC 1 cut(s) 808
Bsh1236I CGCG 3 cut(s) 476, 749, 924
Bsh1285I CGRYCG 2 cut(s) 611, 941
BshFI GGCC 4 cut(s) 22, 479, 594, 1027
BshNI GGYRCC 1 cut(s) 803
BshTI ACCGGT 1 cut(s) 936
BsiEI CGRYCG 2 cut(s) 611, 941
BsiHKAI GWGCWC 1 cut(s) 496
BsiHKCI CYCGRG 2 cut(s) 52, 779
BsiSI CCGG 9 cut(s) 24, 253, 342, 425, 534, 673, 760, 838, 937
BslFI GGGAC 3 cut(s) 165, 653, 1000
BslI CCNNNNNNNGG 3 cut(s) 786, 1010, 1023
BsmAI GTCTC 3 cut(s) 223, 243, 512
BsmFI GGGAC 3 cut(s) 165, 653, 1000
BsmI GAATGC 2 cut(s) 162, 346
BsnI GGCC 4 cut(s) 22, 479, 594, 1027
Bso31I GGTCTC 1 cut(s) 223
BsoBI CYCGRG 2 cut(s) 52, 779
Bsp1286I GDGCHC 2 cut(s) 496, 806
Bsp143I GATC 3 cut(s) 608, 877, 925
BspACI CCGC 3 cut(s) 308, 476, 859
BspANI GGCC 4 cut(s) 22, 479, 594, 1027
BspFNI CGCG 3 cut(s) 476, 749, 924
BspLI GGNNCC 5 cut(s) 677, 805, 945, 1015, 1016
BspPI GGATC 2 cut(s) 872, 920
BspQI GCTCTTC 1 cut(s) 621
BspT107I GGYRCC 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 223
BsrBI CCGCTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 116
BsrFI RCCGGY 4 cut(s) 252, 533, 672, 936
BsrI ACTGG 2 cut(s) 566, 694
BssAI RCCGGY 4 cut(s) 252, 533, 672, 936
BssECI CCNNGG 2 cut(s) 1010, 1022
BssMI GATC 3 cut(s) 608, 877, 925
Bst2UI CCWGG 1 cut(s) 1011
Bst4CI ACNGT 3 cut(s) 644, 715, 908
Bst6I CTCTTC 3 cut(s) 520, 621, 665
BstBAI YACGTR 1 cut(s) 799
BstC8I GCNNGC 2 cut(s) 173, 633
BstDEI CTNAG 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 1022
BstEII GGTNACC 1 cut(s) 111
BstENI CCTNNNNNAGG 1 cut(s) 1008
BstF5I GGATG 3 cut(s) 195, 590, 893
BstFNI CGCG 3 cut(s) 476, 749, 924
BstHHI GCGC 2 cut(s) 751, 924
BstKTI GATC 3 cut(s) 611, 880, 928
BstMAI GTCTC 3 cut(s) 223, 243, 512
BstMBI GATC 3 cut(s) 608, 877, 925
BstMCI CGRYCG 2 cut(s) 611, 941
BstMWI GCNNNNNNNGC 2 cut(s) 830, 1033
BstNI CCWGG 1 cut(s) 1011
BstNSI RCATGY 1 cut(s) 635
BstPI GGTNACC 1 cut(s) 111
BstSCI CCNGG 6 cut(s) 22, 341, 424, 759, 836, 1009
BstSLI GKGCMC 1 cut(s) 806
BstUI CGCG 3 cut(s) 476, 749, 924
BstV1I GCAGC 4 cut(s) 162, 421, 751, 845
BstX2I RGATCY 1 cut(s) 877
BstYI RGATCY 1 cut(s) 877
BsuRI GGCC 4 cut(s) 22, 479, 594, 1027
BtgI CCRYGG 1 cut(s) 1022
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 3 cut(s) 195, 590, 893
BtsI GCAGTG 1 cut(s) 550
BtsIMutI CAGTG 2 cut(s) 550, 670
Cac8I GCNNGC 2 cut(s) 173, 633
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 2 cut(s) 751, 924
Cfr10I RCCGGY 4 cut(s) 252, 533, 672, 936
Cfr13I GGNCC 3 cut(s) 21, 536, 1014
CseI GACGC 1 cut(s) 167
CspAI ACCGGT 1 cut(s) 936
CviAII CATG 2 cut(s) 334, 632
DdeI CTNAG 1 cut(s) 33
DpnI GATC 3 cut(s) 610, 879, 927
DpnII GATC 3 cut(s) 608, 877, 925
EaeI YGGCCR 2 cut(s) 477, 592
Eam1104I CTCTTC 3 cut(s) 520, 621, 665
EarI CTCTTC 3 cut(s) 520, 621, 665
Ecl136II GAGCTC 1 cut(s) 494
Eco24I GRGCYC 1 cut(s) 496
Eco31I GGTCTC 1 cut(s) 223
Eco47I GGWCC 2 cut(s) 536, 1014
Eco53kI GAGCTC 1 cut(s) 494
Eco57I CTGAAG 1 cut(s) 646
Eco88I CYCGRG 2 cut(s) 52, 779
Eco91I GGTNACC 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 494
EcoNI CCTNNNNNAGG 1 cut(s) 1008
EcoO109I RGGNCCY 1 cut(s) 1014
EcoO65I GGTNACC 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 496
FaeI CATG 2 cut(s) 337, 635
FaqI GGGAC 3 cut(s) 165, 653, 1000
FatI CATG 2 cut(s) 333, 631
FauNDI CATATG 1 cut(s) 883
Fnu4HI GCNGC 5 cut(s) 176, 435, 477, 765, 834
FokI GGATG 3 cut(s) 182, 597, 900
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 5 cut(s) 176, 435, 477, 765, 834
FspBI CTAG 4 cut(s) 300, 329, 360, 917
GlaI GCGC 2 cut(s) 750, 923
GluI GCNGC 5 cut(s) 176, 435, 477, 765, 834
GsaI CCCAGC 1 cut(s) 812
GsuI CTGGAG 1 cut(s) 305
HaeIII GGCC 4 cut(s) 22, 479, 594, 1027
HapII CCGG 9 cut(s) 24, 253, 342, 425, 534, 673, 760, 838, 937
HgaI GACGC 1 cut(s) 167
HhaI GCGC 2 cut(s) 751, 924
Hin1II CATG 2 cut(s) 337, 635
Hin6I GCGC 2 cut(s) 749, 922
HinP1I GCGC 2 cut(s) 749, 922
HpaII CCGG 9 cut(s) 24, 253, 342, 425, 534, 673, 760, 838, 937
HphI GGTGA 3 cut(s) 71, 105, 926
Hpy166II GTNNAC 1 cut(s) 899
Hpy188I TCNGA 3 cut(s) 149, 680, 986
Hpy188III TCNNGA 5 cut(s) 78, 313, 322, 779, 965
Hpy8I GTNNAC 1 cut(s) 899
HpyAV CCTTC 5 cut(s) 49, 455, 509, 968, 1028
HpyCH4III ACNGT 3 cut(s) 644, 715, 908
HpyCH4IV ACGT 2 cut(s) 288, 798
HpyCH4V TGCA 5 cut(s) 160, 171, 635, 767, 824
HpyF10VI GCNNNNNNNGC 2 cut(s) 830, 1033
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 2 cut(s) 288, 798
Hsp92II CATG 2 cut(s) 337, 635
HspAI GCGC 2 cut(s) 749, 922
KflI GGGWCCC 1 cut(s) 1014
Kzo9I GATC 3 cut(s) 608, 877, 925
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 2 cut(s) 315, 1006
Lsp1109I GCAGC 4 cut(s) 162, 421, 751, 845
LweI GCATC 2 cut(s) 520, 760
MaeI CTAG 4 cut(s) 300, 329, 360, 917
MaeII ACGT 2 cut(s) 288, 798
MaeIII GTNAC 2 cut(s) 111, 557
MalI GATC 3 cut(s) 610, 879, 927
MbiI CCGCTC 1 cut(s) 310
MboI GATC 3 cut(s) 608, 877, 925
MboII GAAGA 8 cut(s) 288, 537, 611, 638, 647, 652, 784, 887
MfeI CAATTG 1 cut(s) 768
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 2 cut(s) 496, 806
MlsI TGGCCA 1 cut(s) 594
MluCI AATT 6 cut(s) 222, 465, 768, 813, 866, 969
MluNI TGGCCA 1 cut(s) 594
MlyI GAGTC 4 cut(s) 416, 514, 791, 856
MnlI CCTC 9 cut(s) 102, 209, 382, 541, 549, 606, 711, 730, 887
Mox20I TGGCCA 1 cut(s) 594
MscI TGGCCA 1 cut(s) 594
MseI TTAA 1 cut(s) 1042
MslI CAYNNNNRTG 2 cut(s) 131, 886
Msp20I TGGCCA 1 cut(s) 594
MspI CCGG 9 cut(s) 24, 253, 342, 425, 534, 673, 760, 838, 937
MspR9I CCNGG 6 cut(s) 24, 343, 426, 761, 838, 1011
MunI CAATTG 1 cut(s) 768
Mva1269I GAATGC 2 cut(s) 162, 346
MvaI CCWGG 1 cut(s) 1011
MvnI CGCG 3 cut(s) 476, 749, 924
MwoI GCNNNNNNNGC 2 cut(s) 830, 1033
NciI CCSGG 5 cut(s) 24, 343, 426, 761, 838
NdeI CATATG 1 cut(s) 883
NdeII GATC 3 cut(s) 608, 877, 925
NlaIII CATG 2 cut(s) 337, 635
NlaIV GGNNCC 5 cut(s) 677, 805, 945, 1015, 1016
NmuCI GTSAC 1 cut(s) 111
NspI RCATGY 1 cut(s) 635
PaeI GCATGC 1 cut(s) 635
PciSI GCTCTTC 1 cut(s) 621
PctI GAATGC 2 cut(s) 162, 346
PfeI GAWTC 8 cut(s) 74, 316, 325, 392, 421, 575, 652, 744
PinAI ACCGGT 1 cut(s) 936
PkrI GCNGC 5 cut(s) 177, 436, 478, 766, 835
Ple19I CGATCG 1 cut(s) 611
PleI GAGTC 4 cut(s) 415, 514, 790, 856
PpsI GAGTC 4 cut(s) 415, 514, 790, 856
Ppu21I YACGTR 1 cut(s) 799
PpuMI RGGWCCY 1 cut(s) 1014
Psp124BI GAGCTC 1 cut(s) 496
Psp5II RGGWCCY 1 cut(s) 1014
Psp6I CCWGG 1 cut(s) 1009
PspEI GGTNACC 1 cut(s) 111
PspFI CCCAGC 1 cut(s) 808
PspGI CCWGG 1 cut(s) 1009
PspN4I GGNNCC 5 cut(s) 677, 805, 945, 1015, 1016
PspPI GGNCC 3 cut(s) 21, 536, 1014
PspPPI RGGWCCY 1 cut(s) 1014
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 1 cut(s) 877
PvuI CGATCG 1 cut(s) 611
RseI CAYNNNNRTG 2 cut(s) 131, 886
SacI GAGCTC 1 cut(s) 496
SapI GCTCTTC 1 cut(s) 621
SaqAI TTAA 1 cut(s) 1042
SatI GCNGC 5 cut(s) 176, 435, 477, 765, 834
Sau3AI GATC 3 cut(s) 608, 877, 925
Sau96I GGNCC 3 cut(s) 21, 536, 1014
SchI GAGTC 4 cut(s) 416, 514, 791, 856
ScrFI CCNGG 6 cut(s) 24, 343, 426, 761, 838, 1011
SduI GDGCHC 2 cut(s) 496, 806
SfaNI GCATC 2 cut(s) 520, 760
SinI GGWCC 2 cut(s) 536, 1014
SmiMI CAYNNNNRTG 2 cut(s) 131, 886
SpeI ACTAGT 1 cut(s) 359
SphI GCATGC 1 cut(s) 635
Sse9I AATT 6 cut(s) 222, 465, 768, 813, 866, 969
SsiI CCGC 3 cut(s) 308, 476, 859
SspMI CTAG 4 cut(s) 300, 329, 360, 917
SstI GAGCTC 1 cut(s) 496
StyD4I CCNGG 6 cut(s) 22, 341, 424, 759, 836, 1009
TaaI ACNGT 3 cut(s) 644, 715, 908
TaiI ACGT 2 cut(s) 291, 801
TaqI TCGA 3 cut(s) 523, 611, 892
TasI AATT 6 cut(s) 222, 465, 768, 813, 866, 969
TauI GCSGC 1 cut(s) 479
TfiI GAWTC 8 cut(s) 74, 316, 325, 392, 421, 575, 652, 744
Tru1I TTAA 1 cut(s) 1042
Tru9I TTAA 1 cut(s) 1042
TscAI CASTG 2 cut(s) 550, 670
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 4 cut(s) 175, 434, 764, 833
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 1 cut(s) 184
TspGWI ACGGA 2 cut(s) 918, 1011
TspRI CASTG 2 cut(s) 550, 670
VpaK11BI GGWCC 2 cut(s) 536, 1014
XagI CCTNNNNNAGG 1 cut(s) 1008
XceI RCATGY 1 cut(s) 635
XspI CTAG 4 cut(s) 300, 329, 360, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.