Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0015783

Physical Location & Seq

Genomic Coordinates Reverse (-)

14557062 .. 14560744

Gene Structure 3683 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g16680.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1269 bp

ATGGGGAATTGCTGGTTTTCTGCTTCAGCTGAAGACCCAACACCTGCTACTACTGGTCATCTCAGCACAGCTGCGAGTAATGTATCTCAATCGACCAGTAATACCATTTCCACTTCTTCTGGGGGAAGCAGTGTATCCAGGAACAGCCAGTTTTCGGCAAGAAGTGGCGATGAGACCTGTCCAGATGGGCAGATTCTACCCGATGCAAACTTGAGAATCTTCAGTTATCTGGAATTGAAGAGTGCAACTCGGAACTTCAGATCAGACACGGTACTTGGGGAAGGAGGTTTTGGGAAAGTCTATAAAGGTTGGCTTGAGGAGAGGCCAGCCATGAAACCGGGAAGGACAACTGTAATTGCTGTAAAGAAATTGAATTCGGAGAGCTTGCAAGGGTTTGAGGAATGGCAGTCAGAGGTGAATTTCTTGGGTAGACTTTCTCATCCTAATCTTGTAAAGCTGTTGGGGTACTGTTTGGAGGAGAAAGAACTGCTGCTTGTCTATGAGTTCATGCAAAAAGGAAGCTTGGAAAACCATCTTTTTGGAAGGGGCTCAGTTGTTCAGCCACTTCCATGGGACATACGGCTTAAAGTTGCAATAGGAGCAGCTCGAGGCCTGGCATTTTTGCATACATCAGAGAAGCAAGTAATCTATAGAGATTTCAAAGCTTCCAATATACTACTTGACGGGTCCTTTAATGCCAAGATATCCGACTTTGGCTTGGCAAAATTGGGTCCTTCAGAGAGTCAATCGCATGTGACAACCCGGGTCATGGGCACATATGGTTATGCTGCTCCTGAGTATGTTTCCACAGGGCATCTTTATGTGAAGAGCGATGTGTACGGTTTTGGTGTTGTGCTGGTTGAGATCTTAACAGGCTTACGAGCACTTGATACGAATCGTCCAAGTGGAAGACATAATCTTGTGGAGTGGATAAAACCATTTTTATCAGATAAAAGAAAATTGAAAGGCATTATGGACCCTCGACTGGAGGCAAAGTATCCAGCCAAAGCTGCATTCCGTATTTCCCAGCTTGCTCTAAAATGCATTGAATCTGAACACAAAAACCGGCCATCAATGATAGATGTTGTGGAGACTTTGGAAAGGATCCAGTCAGTGAATGAAGTTATAAGGGAACCTAGGATTCGTTCCAATCGTTCTACTCATCCTATGGCTCATCGCCAAGGTCAGACACCTTTGCACTATCGTTCTCCACTTCACCCCAGGCCAGATGAAAATCAAAACTACCAAGGGTCGCCTCGAATGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

423

Amino Acids

46.98

Weight (kDa)

9.32

Isoelectric Point (pI)

38.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	88 - 364	1.7e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	89 - 366	2.7e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015783)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G17220 AT2G17220
fragaria_vesca	FvH4_2g16680
malus_domestica	MD05G1135000.v1.1 MD10G1140500.v1.1
prunus_persica	Prupe.8G178900_v2.0.a1
pyrus_communis	pycom05g13160 pycom10g11910
rosa_chinensis	RchiOBHm_Chr6g0280821
rosa_multiflora	Rmu_co8480431.1_g000001
rosa_roxburghii	Rroxscaffold_7G00187600
rosa_rugosa	Rorug06G0134700
rosa_samantha	Rh6BG249600 Rh6DG242400
rosa_wichuraiana	Rw6G021440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1127
AarI	CACCTGC	1 cut(s)	52
Acc36I	ACCTGC	1 cut(s)	52
AccI	GTMKAC	1 cut(s)	430
AclWI	GGATC	2 cut(s)	1099, 1112
AcoI	YGGCCR	1 cut(s)	1067
AcsI	RAATTY	2 cut(s)	373, 418
AcuI	CTGAAG	5 cut(s)	9, 51, 205, 241, 720
AfaI	GTAC	3 cut(s)	273, 467, 839
AfiI	CCNNNNNNNGG	3 cut(s)	154, 769, 985
AgsI	TTSAA	5 cut(s)	238, 373, 661, 964, 1049
AjnI	CCWGG	3 cut(s)	137, 612, 1220
AjuI	GAANNNNNNNTTGG	4 cut(s)	273, 305, 998, 1030
AluBI	AGCT	9 cut(s)	29, 71, 384, 457, 522, 605, 665, 1010, 1030
AluI	AGCT	9 cut(s)	29, 71, 384, 457, 522, 605, 665, 1010, 1030
Alw21I	GWGCWC	1 cut(s)	886
Alw26I	GTCTC	2 cut(s)	167, 1085
AlwI	GGATC	2 cut(s)	1099, 1112
Ama87I	CYCGRG	2 cut(s)	606, 762
AoxI	GGCC	4 cut(s)	323, 610, 1067, 1223
ApeKI	GCWGC	5 cut(s)	71, 490, 602, 788, 1010
ApoI	RAATTY	2 cut(s)	373, 418
AspA2I	CCTAGG	1 cut(s)	1136
AspS9I	GGNCC	3 cut(s)	687, 731, 976
AsuC2I	CCSGG	3 cut(s)	339, 763, 764
AsuHPI	GGTGA	2 cut(s)	427, 1208
AvaI	CYCGRG	2 cut(s)	606, 762
AvaII	GGWCC	3 cut(s)	687, 731, 976
AvrII	CCTAGG	1 cut(s)	1136
BaeGI	GKGCMC	1 cut(s)	776
BamHI	GGATCC	1 cut(s)	1104
BanII	GRGCYC	1 cut(s)	551
BbsI	GAAGAC	2 cut(s)	39, 916
Bbv12I	GWGCWC	1 cut(s)	886
BbvI	GCAGC	5 cut(s)	58, 477, 614, 775, 997
BccI	CCATC	3 cut(s)	179, 540, 1078
BceAI	ACGGC	1 cut(s)	596
BciT130I	CCWGG	3 cut(s)	139, 614, 1222
BciVI	GTATCC	2 cut(s)	145, 1008
BcnI	CCSGG	3 cut(s)	339, 763, 764
BcoDI	GTCTC	2 cut(s)	167, 1085
BfaI	CTAG	1 cut(s)	1137
BfmI	CTRYAG	1 cut(s)	649
BfuAI	ACCTGC	1 cut(s)	52
BfuI	GTATCC	2 cut(s)	145, 1008
BglII	AGATCT	1 cut(s)	864
BisI	GCNGC	5 cut(s)	72, 491, 603, 789, 1011
BlnI	CCTAGG	1 cut(s)	1136
BlsI	GCNGC	5 cut(s)	73, 492, 604, 790, 1012
Bme1390I	CCNGG	6 cut(s)	139, 339, 614, 763, 764, 1222
Bme18I	GGWCC	3 cut(s)	687, 731, 976
BmeT110I	CYCGRG	2 cut(s)	606, 762
BmgT120I	GGNCC	3 cut(s)	687, 731, 976
BmiI	GGNNCC	5 cut(s)	688, 732, 978, 1106, 1134
BmrFI	CCNGG	6 cut(s)	139, 339, 614, 763, 764, 1222
BmsI	GCATC	2 cut(s)	193, 823
BpiI	GAAGAC	2 cut(s)	39, 916
BplI	GAGNNNNNCTC	2 cut(s)	232, 264
BpmI	CTGGAG	1 cut(s)	1007
BpuEI	CTTGAG	2 cut(s)	232, 335
BpuMI	CCSGG	3 cut(s)	339, 763, 764
BsaBI	GATNNNNATC	2 cut(s)	894, 1233
BsaI	GGTCTC	1 cut(s)	167
BsaJI	CCNNGG	6 cut(s)	569, 762, 1136, 1180, 1220, 1246
BsaXI	ACNNNNNCTCC	2 cut(s)	980, 1010
Bsc4I	CCNNNNNNNGG	3 cut(s)	154, 769, 985
Bse118I	RCCGGY	1 cut(s)	1065
Bse1I	ACTGG	5 cut(s)	58, 96, 148, 990, 1108
Bse8I	GATNNNNATC	2 cut(s)	894, 1233
BseBI	CCWGG	3 cut(s)	139, 614, 1222
BseDI	CCNNGG	6 cut(s)	569, 762, 1136, 1180, 1220, 1246
BseGI	GGATG	2 cut(s)	439, 1162
BseJI	GATNNNNATC	2 cut(s)	894, 1233
BseLI	CCNNNNNNNGG	3 cut(s)	154, 769, 985
BseMII	CTCAG	3 cut(s)	76, 564, 786
BseNI	ACTGG	5 cut(s)	58, 96, 148, 990, 1108
BseRI	GAGGAG	2 cut(s)	332, 491
BseSI	GKGCMC	1 cut(s)	776
BseXI	GCAGC	5 cut(s)	58, 477, 614, 775, 997
BseYI	CCCAGC	1 cut(s)	1026
BshFI	GGCC	4 cut(s)	325, 612, 1069, 1225
BsiHKAI	GWGCWC	1 cut(s)	886
BsiHKCI	CYCGRG	2 cut(s)	606, 762
BsiSI	CCGG	3 cut(s)	338, 763, 1066
BslFI	GGGAC	1 cut(s)	587
BslI	CCNNNNNNNGG	3 cut(s)	154, 769, 985
BsmAI	GTCTC	2 cut(s)	167, 1085
BsmFI	GGGAC	1 cut(s)	587
BsmI	GAATGC	1 cut(s)	1013
BsnI	GGCC	4 cut(s)	325, 612, 1069, 1225
Bso31I	GGTCTC	1 cut(s)	167
BsoBI	CYCGRG	2 cut(s)	606, 762
Bsp1286I	GDGCHC	3 cut(s)	551, 776, 886
Bsp143I	GATC	3 cut(s)	260, 864, 1104
Bsp19I	CCATGG	1 cut(s)	569
BspANI	GGCC	4 cut(s)	325, 612, 1069, 1225
BspCNI	CTCAG	3 cut(s)	75, 563, 787
BspLI	GGNNCC	5 cut(s)	688, 732, 978, 1106, 1134
BspMI	ACCTGC	1 cut(s)	52
BspPI	GGATC	2 cut(s)	1099, 1112
BspQI	GCTCTTC	1 cut(s)	821
BspTNI	GGTCTC	1 cut(s)	167
BsrFI	RCCGGY	1 cut(s)	1065
BsrI	ACTGG	5 cut(s)	58, 96, 148, 990, 1108
BssAI	RCCGGY	1 cut(s)	1065
BssECI	CCNNGG	6 cut(s)	569, 762, 1136, 1180, 1220, 1246
BssMI	GATC	3 cut(s)	260, 864, 1104
BssT1I	CCWWGG	4 cut(s)	569, 1136, 1180, 1246
Bst2UI	CCWGG	3 cut(s)	139, 614, 1222
Bst4CI	ACNGT	4 cut(s)	271, 352, 470, 842
Bst6I	CTCTTC	2 cut(s)	233, 821
BstC8I	GCNNGC	3 cut(s)	327, 386, 1032
BstDEI	CTNAG	3 cut(s)	62, 550, 795
BstDSI	CCRYGG	1 cut(s)	569
BstF5I	GGATG	2 cut(s)	439, 1162
BstKTI	GATC	3 cut(s)	263, 867, 1107
BstMAI	GTCTC	2 cut(s)	167, 1085
BstMBI	GATC	3 cut(s)	260, 864, 1104
BstMWI	GCNNNNNNNGC	2 cut(s)	599, 1010
BstNI	CCWGG	3 cut(s)	139, 614, 1222
BstNSI	RCATGY	1 cut(s)	755
BstSCI	CCNGG	6 cut(s)	137, 337, 612, 761, 762, 1220
BstSFI	CTRYAG	1 cut(s)	649
BstSLI	GKGCMC	1 cut(s)	776
BstV1I	GCAGC	5 cut(s)	58, 477, 614, 775, 997
BstV2I	GAAGAC	2 cut(s)	39, 916
BstX2I	RGATCY	2 cut(s)	864, 1104
BstXI	CCANNNNNNTGG	2 cut(s)	539, 570
BstYI	RGATCY	2 cut(s)	864, 1104
BsuI	GTATCC	2 cut(s)	145, 1008
BsuRI	GGCC	4 cut(s)	325, 612, 1069, 1225
BtgI	CCRYGG	1 cut(s)	569
BtgZI	GCGATG	3 cut(s)	183, 846, 1160
BtsCI	GGATG	2 cut(s)	439, 1162
BtsI	GCAGTG	1 cut(s)	136
BtsIMutI	CAGTG	2 cut(s)	136, 1119
BveI	ACCTGC	1 cut(s)	52
Cac8I	GCNNGC	3 cut(s)	327, 386, 1032
Cfr10I	RCCGGY	1 cut(s)	1065
Cfr13I	GGNCC	3 cut(s)	687, 731, 976
Cfr9I	CCCGGG	1 cut(s)	762
Csp6I	GTAC	3 cut(s)	272, 466, 838
CviAII	CATG	5 cut(s)	331, 508, 570, 752, 769
CviQI	GTAC	3 cut(s)	272, 466, 838
DdeI	CTNAG	3 cut(s)	62, 550, 795
DpnI	GATC	3 cut(s)	262, 866, 1106
DpnII	GATC	3 cut(s)	260, 864, 1104
EaeI	YGGCCR	1 cut(s)	1067
Eam1104I	CTCTTC	2 cut(s)	233, 821
EarI	CTCTTC	2 cut(s)	233, 821
Eco130I	CCWWGG	4 cut(s)	569, 1136, 1180, 1246
Eco147I	AGGCCT	1 cut(s)	612
Eco24I	GRGCYC	1 cut(s)	551
Eco31I	GGTCTC	1 cut(s)	167
Eco32I	GATATC	1 cut(s)	705
Eco47I	GGWCC	3 cut(s)	687, 731, 976
Eco57I	CTGAAG	5 cut(s)	9, 51, 205, 241, 720
Eco88I	CYCGRG	2 cut(s)	606, 762
EcoO109I	RGGNCCY	2 cut(s)	687, 731
EcoRI	GAATTC	1 cut(s)	373
EcoRII	CCWGG	3 cut(s)	137, 612, 1220
EcoRV	GATATC	1 cut(s)	705
EcoT14I	CCWWGG	4 cut(s)	569, 1136, 1180, 1246
EcoT22I	ATGCAT	1 cut(s)	1046
EcoT38I	GRGCYC	1 cut(s)	551
ErhI	CCWWGG	4 cut(s)	569, 1136, 1180, 1246
FaeI	CATG	5 cut(s)	334, 511, 573, 755, 772
FalI	AAGNNNNNCTT	2 cut(s)	297, 329
FaqI	GGGAC	1 cut(s)	587
FatI	CATG	5 cut(s)	330, 507, 569, 751, 768
FauNDI	CATATG	1 cut(s)	778
FblI	GTMKAC	1 cut(s)	430
Fnu4HI	GCNGC	5 cut(s)	72, 491, 603, 789, 1011
FokI	GGATG	2 cut(s)	426, 1149
FriOI	GRGCYC	1 cut(s)	551
Fsp4HI	GCNGC	5 cut(s)	72, 491, 603, 789, 1011
FspBI	CTAG	1 cut(s)	1137
GluI	GCNGC	5 cut(s)	72, 491, 603, 789, 1011
GsaI	CCCAGC	1 cut(s)	1030
GsuI	CTGGAG	1 cut(s)	1007
HaeIII	GGCC	4 cut(s)	325, 612, 1069, 1225
HapII	CCGG	3 cut(s)	338, 763, 1066
Hin1II	CATG	5 cut(s)	334, 511, 573, 755, 772
HindIII	AAGCTT	2 cut(s)	520, 663
HinfI	GANTC	6 cut(s)	193, 216, 742, 895, 1049, 1141
HpaII	CCGG	3 cut(s)	338, 763, 1066
HphI	GGTGA	2 cut(s)	427, 1208
Hpy166II	GTNNAC	2 cut(s)	431, 838
Hpy188III	TCNNGA	3 cut(s)	182, 230, 794
Hpy8I	GTNNAC	2 cut(s)	431, 838
HpyAV	CCTTC	4 cut(s)	275, 336, 537, 744
HpyCH4III	ACNGT	4 cut(s)	271, 352, 470, 842
HpyCH4V	TGCA	9 cut(s)	206, 245, 388, 511, 593, 625, 1013, 1044, 1198
HpyF10VI	GCNNNNNNNGC	2 cut(s)	599, 1010
HpyF3I	CTNAG	3 cut(s)	62, 550, 795
Hsp92II	CATG	5 cut(s)	334, 511, 573, 755, 772
Kzo9I	GATC	3 cut(s)	260, 864, 1104
LguI	GCTCTTC	1 cut(s)	821
LmnI	GCTCC	2 cut(s)	599, 796
Lsp1109I	GCAGC	5 cut(s)	58, 477, 614, 775, 997
LweI	GCATC	2 cut(s)	193, 823
MaeI	CTAG	1 cut(s)	1137
MaeIII	GTNAC	1 cut(s)	754
MalI	GATC	3 cut(s)	262, 866, 1106
MboI	GATC	3 cut(s)	260, 864, 1104
MboII	GAAGA	6 cut(s)	44, 108, 211, 250, 838, 921
MflI	RGATCY	2 cut(s)	864, 1104
MhlI	GDGCHC	3 cut(s)	551, 776, 886
MluCI	AATT	8 cut(s)	7, 233, 354, 368, 373, 418, 725, 959
MlyI	GAGTC	1 cut(s)	751
MmeI	TCCRAC	1 cut(s)	732
Mph1103I	ATGCAT	1 cut(s)	1046
MseI	TTAA	3 cut(s)	585, 693, 869
MslI	CAYNNNNRTG	2 cut(s)	568, 819
MspA1I	CMGCKG	2 cut(s)	29, 71
MspI	CCGG	3 cut(s)	338, 763, 1066
MspR9I	CCNGG	6 cut(s)	139, 339, 614, 763, 764, 1222
Mva1269I	GAATGC	1 cut(s)	1013
MvaI	CCWGG	3 cut(s)	139, 614, 1222
MwoI	GCNNNNNNNGC	2 cut(s)	599, 1010
NciI	CCSGG	3 cut(s)	339, 763, 764
NcoI	CCATGG	1 cut(s)	569
NdeI	CATATG	1 cut(s)	778
NdeII	GATC	3 cut(s)	260, 864, 1104
NlaIII	CATG	5 cut(s)	334, 511, 573, 755, 772
NlaIV	GGNNCC	5 cut(s)	688, 732, 978, 1106, 1134
NmuCI	GTSAC	1 cut(s)	754
NsiI	ATGCAT	1 cut(s)	1046
NspI	RCATGY	1 cut(s)	755
PaeR7I	CTCGAG	1 cut(s)	606
PaqCI	CACCTGC	1 cut(s)	52
PceI	AGGCCT	1 cut(s)	612
PciSI	GCTCTTC	1 cut(s)	821
PcsI	WCGNNNNNNNCGW	1 cut(s)	1150
PctI	GAATGC	1 cut(s)	1013
PfeI	GAWTC	5 cut(s)	193, 216, 895, 1049, 1141
PfoI	TCCNGGA	1 cut(s)	137
PkrI	GCNGC	5 cut(s)	73, 492, 604, 790, 1012
PleI	GAGTC	1 cut(s)	750
PpsI	GAGTC	1 cut(s)	750
PpuMI	RGGWCCY	2 cut(s)	687, 731
PsiI	TTATAA	1 cut(s)	1127
Psp5II	RGGWCCY	2 cut(s)	687, 731
Psp6I	CCWGG	3 cut(s)	137, 612, 1220
PspFI	CCCAGC	1 cut(s)	1026
PspGI	CCWGG	3 cut(s)	137, 612, 1220
PspN4I	GGNNCC	5 cut(s)	688, 732, 978, 1106, 1134
PspPI	GGNCC	3 cut(s)	687, 731, 976
PspPPI	RGGWCCY	2 cut(s)	687, 731
PspXI	VCTCGAGB	1 cut(s)	606
PsuI	RGATCY	2 cut(s)	864, 1104
PvuII	CAGCTG	2 cut(s)	29, 71
RsaI	GTAC	3 cut(s)	273, 467, 839
RsaNI	GTAC	3 cut(s)	272, 466, 838
RseI	CAYNNNNRTG	2 cut(s)	568, 819
SapI	GCTCTTC	1 cut(s)	821
SaqAI	TTAA	3 cut(s)	585, 693, 869
SatI	GCNGC	5 cut(s)	72, 491, 603, 789, 1011
Sau3AI	GATC	3 cut(s)	260, 864, 1104
Sau96I	GGNCC	3 cut(s)	687, 731, 976
SchI	GAGTC	1 cut(s)	751
ScrFI	CCNGG	6 cut(s)	139, 339, 614, 763, 764, 1222
SduI	GDGCHC	3 cut(s)	551, 776, 886
SfaNI	GCATC	2 cut(s)	193, 823
SfcI	CTRYAG	1 cut(s)	649
Sfr274I	CTCGAG	1 cut(s)	606
SinI	GGWCC	3 cut(s)	687, 731, 976
SlaI	CTCGAG	1 cut(s)	606
SmaI	CCCGGG	1 cut(s)	764
SmiMI	CAYNNNNRTG	2 cut(s)	568, 819
SmlI	CTYRAG	3 cut(s)	211, 314, 606
SmoI	CTYRAG	3 cut(s)	211, 314, 606
Sse9I	AATT	8 cut(s)	7, 233, 354, 368, 373, 418, 725, 959
SseBI	AGGCCT	1 cut(s)	612
SspMI	CTAG	1 cut(s)	1137
StuI	AGGCCT	1 cut(s)	612
StyD4I	CCNGG	6 cut(s)	137, 337, 612, 761, 762, 1220
StyI	CCWWGG	4 cut(s)	569, 1136, 1180, 1246
TaaI	ACNGT	4 cut(s)	271, 352, 470, 842
TaqI	TCGA	4 cut(s)	92, 607, 982, 1258
TasI	AATT	8 cut(s)	7, 233, 354, 368, 373, 418, 725, 959
TfiI	GAWTC	5 cut(s)	193, 216, 895, 1049, 1141
Tru1I	TTAA	3 cut(s)	585, 693, 869
Tru9I	TTAA	3 cut(s)	585, 693, 869
TscAI	CASTG	2 cut(s)	136, 1119
TseFI	GTSAC	1 cut(s)	754
TseI	GCWGC	5 cut(s)	71, 490, 602, 788, 1010
Tsp45I	GTSAC	1 cut(s)	754
TspDTI	ATGAA	4 cut(s)	347, 496, 1134, 1245
TspGWI	ACGGA	1 cut(s)	1007
TspMI	CCCGGG	1 cut(s)	762
TspRI	CASTG	2 cut(s)	136, 1119
VpaK11BI	GGWCC	3 cut(s)	687, 731, 976
XapI	RAATTY	2 cut(s)	373, 418
XceI	RCATGY	1 cut(s)	755
XhoI	CTCGAG	1 cut(s)	606
XmaI	CCCGGG	1 cut(s)	762
XmaJI	CCTAGG	1 cut(s)	1136
XmiI	GTMKAC	1 cut(s)	430
XspI	CTAG	1 cut(s)	1137
Zsp2I	ATGCAT	1 cut(s)	1046

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.