FvH4_2g19050

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017600
Physical Location & Seq
Forward (+)
16221143 .. 16221961
819 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19050.t1

Sequence Viewer

Length: 819 bp
ATGGGAACCATAGCACAGGAATCAGGCGTGCTGAAGCTGGTGCGCCCTGGCAGGCGAGTGGAGCGTTATGAGGTACCAATTACTGCAGCTGAGGTCATGAGAAGGTACCCCAGGCACAGCATCACTCGGCCGGATATCTTTGAGTTCCCATGGATTTTGGTTAAGCCTGAAGCAATTTTGACCCCTGGAAGAGTGTTCTACATAGTTCCTAACCGCACTATCTATCAGTTGCTTAAAGCTAGAGAGAATTCTGATTCTGATCAGTCATCTCCTGCTTTGCAGCAGCAGCAGCAACGAGAGTTTCCAAAAGATCAAGCTCAGCAGCAAGTATCAAAAAAGACTTCTCCATCCAACAAGTCAGACGCTGGGAGGACACCAAAGCACCAAGAGCACAGGAAGCGGCTTAAGCATCAATTCTTGAACATGTCTTGTGCTACTACTTCTGTGTGTCCAACTGAAGAACAAGACTATGACAAAGAAGTACGAAGAGCTGTCCAAAGGGTGTCATCTGCTCCAGTGTTGCTAGACTCCAAAAAAACTCATGAAGAGCCTAAACGAAAACCATCATCTCATTCTTCAAAAAATGAGAATAGAAAAGGTAAAGGTAAAGCTAGCTTCAGTAGCATTACTAGCAAACTTGGTGATCTAAAGGTTACAACTAGTAGACAGGTTCCCACACTGAAATCTTGTATAAGAAAACCAGATAGTGCTCGTAAGTTGCGTCAATTTATAGTGAGATTTGAGTTGCCAGACGACTATGTGGAGCGGCAAACCAAGGCTGCTGATTATCGGATGCAATATCCAGACTATAAGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

273

Amino Acids

31.46

Weight (kDa)

10.0

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 183 1.4e-14 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19050
malus_domestica MD05G1175300.v1.1 MD10G1164400.v1.1
prunus_persica Prupe.8G203000_v2.0.a1
rosa_laevigata RLG00000012706
rosa_multiflora Rmu_sc0000686.1_g000018
rosa_roxburghii Rroxscaffold_7G00184280
rosa_rugosa Rorug06G0167600
rosa_samantha Rh6AG280400 Rh6BG280700 Rh6CG282700 Rh6DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 73, 105
AccB1I GGYRCC 2 cut(s) 73, 105
AccBSI CCGCTC 1 cut(s) 766
AccI GTMKAC 1 cut(s) 664
AciI CCGC 3 cut(s) 214, 400, 766
AcoI YGGCCR 1 cut(s) 128
AcsI RAATTY 1 cut(s) 247
AcuI CTGAAG 4 cut(s) 53, 189, 477, 601
AfaI GTAC 3 cut(s) 75, 107, 483
AflII CTTAAG 1 cut(s) 404
AflIII ACRYGT 1 cut(s) 423
AgsI TTSAA 2 cut(s) 421, 579
AhlI ACTAGT 1 cut(s) 659
AjnI CCWGG 3 cut(s) 46, 110, 184
AluBI AGCT 7 cut(s) 37, 89, 239, 317, 491, 611, 615
AluI AGCT 7 cut(s) 37, 89, 239, 317, 491, 611, 615
Alw21I GWGCWC 2 cut(s) 393, 712
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 7 cut(s) 86, 280, 283, 286, 289, 322, 779
ApoI RAATTY 1 cut(s) 247
Asp718I GGTACC 2 cut(s) 73, 105
AspLEI GCGC 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 653
AsuNHI GCTAGC 1 cut(s) 611
BanI GGYRCC 2 cut(s) 73, 105
Bbv12I GWGCWC 2 cut(s) 393, 712
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 7 cut(s) 98, 292, 295, 298, 301, 334, 766
BccI CCATC 2 cut(s) 355, 571
BciT130I CCWGG 3 cut(s) 48, 112, 186
BclI TGATCA 1 cut(s) 259
BcuI ACTAGT 1 cut(s) 659
BfaI CTAG 5 cut(s) 240, 524, 612, 630, 660
BfmI CTRYAG 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 404
BisI GCNGC 9 cut(s) 87, 281, 284, 287, 290, 323, 401, 767, 780
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 9 cut(s) 88, 282, 285, 288, 291, 324, 402, 768, 781
Bme1390I CCNGG 3 cut(s) 48, 112, 186
BmiI GGNNCC 4 cut(s) 7, 75, 107, 672
BmrFI CCNGG 3 cut(s) 48, 112, 186
BmsI GCATC 3 cut(s) 129, 418, 783
BmtI GCTAGC 1 cut(s) 615
BpmI CTGGAG 1 cut(s) 498
Bpu10I CCTNAGC 1 cut(s) 90
Bpu1102I GCTNAGC 1 cut(s) 318
BsaBI GATNNNNATC 1 cut(s) 258
BsaJI CCNNGG 5 cut(s) 46, 110, 149, 184, 774
Bse1I ACTGG 1 cut(s) 515
Bse8I GATNNNNATC 1 cut(s) 258
BseBI CCWGG 3 cut(s) 48, 112, 186
BseDI CCNNGG 5 cut(s) 46, 110, 149, 184, 774
BseGI GGATG 2 cut(s) 347, 798
BseJI GATNNNNATC 1 cut(s) 258
BseMII CTCAG 2 cut(s) 81, 332
BseNI ACTGG 1 cut(s) 515
BseX3I CGGCCG 1 cut(s) 128
BseXI GCAGC 7 cut(s) 98, 292, 295, 298, 301, 334, 766
BseYI CCCAGC 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 131
BshFI GGCC 1 cut(s) 130
BshNI GGYRCC 2 cut(s) 73, 105
BsiEI CGRYCG 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 393, 712
BsiSI CCGG 1 cut(s) 131
BsnI GGCC 1 cut(s) 130
Bsp1286I GDGCHC 2 cut(s) 393, 712
Bsp143I GATC 3 cut(s) 259, 310, 643
Bsp1720I GCTNAGC 1 cut(s) 318
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 3 cut(s) 214, 400, 766
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 2 cut(s) 82, 331
BspHI TCATGA 2 cut(s) 96, 541
BspLI GGNNCC 4 cut(s) 7, 75, 107, 672
BspMAI CTGCAG 1 cut(s) 88
BspOI GCTAGC 1 cut(s) 615
BspQI GCTCTTC 2 cut(s) 481, 540
BspT107I GGYRCC 2 cut(s) 73, 105
BspTI CTTAAG 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 766
BsrI ACTGG 1 cut(s) 515
BssECI CCNNGG 5 cut(s) 46, 110, 149, 184, 774
BssMI GATC 3 cut(s) 259, 310, 643
BssT1I CCWWGG 2 cut(s) 149, 774
Bst2UI CCWGG 3 cut(s) 48, 112, 186
Bst6I CTCTTC 3 cut(s) 184, 481, 540
BstAFI CTTAAG 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 29, 53, 613
BstDEI CTNAG 2 cut(s) 90, 318
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 2 cut(s) 347, 798
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 3 cut(s) 262, 313, 646
BstMBI GATC 3 cut(s) 259, 310, 643
BstMCI CGRYCG 1 cut(s) 131
BstMWI GCNNNNNNNGC 8 cut(s) 61, 286, 289, 388, 397, 406, 621, 630
BstNI CCWGG 3 cut(s) 48, 112, 186
BstNSI RCATGY 1 cut(s) 427
BstSCI CCNGG 3 cut(s) 46, 110, 184
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 7 cut(s) 98, 292, 295, 298, 301, 334, 766
BstZI CGGCCG 1 cut(s) 128
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 2 cut(s) 347, 798
BtsIMutI CAGTG 2 cut(s) 522, 677
Cac8I GCNNGC 3 cut(s) 29, 53, 613
CaiI CAGNNNCTG 1 cut(s) 365
CciI TCATGA 2 cut(s) 96, 541
CfoI GCGC 1 cut(s) 45
CseI GACGC 2 cut(s) 371, 710
Csp6I GTAC 3 cut(s) 74, 106, 482
CviAII CATG 4 cut(s) 97, 150, 424, 542
CviQI GTAC 3 cut(s) 74, 106, 482
DdeI CTNAG 2 cut(s) 90, 318
DpnI GATC 3 cut(s) 261, 312, 645
DpnII GATC 3 cut(s) 259, 310, 643
EaeI YGGCCR 1 cut(s) 128
EagI CGGCCG 1 cut(s) 128
Eam1104I CTCTTC 3 cut(s) 184, 481, 540
EarI CTCTTC 3 cut(s) 184, 481, 540
EclXI CGGCCG 1 cut(s) 128
Eco130I CCWWGG 2 cut(s) 149, 774
Eco32I GATATC 1 cut(s) 136
Eco52I CGGCCG 1 cut(s) 128
Eco57I CTGAAG 4 cut(s) 53, 189, 477, 601
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 3 cut(s) 46, 110, 184
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 149, 774
ErhI CCWWGG 2 cut(s) 149, 774
FaeI CATG 4 cut(s) 100, 153, 427, 545
FatI CATG 4 cut(s) 96, 149, 423, 541
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 1 cut(s) 664
Fnu4HI GCNGC 9 cut(s) 87, 281, 284, 287, 290, 323, 401, 767, 780
FokI GGATG 2 cut(s) 334, 805
Fsp4HI GCNGC 9 cut(s) 87, 281, 284, 287, 290, 323, 401, 767, 780
FspBI CTAG 5 cut(s) 240, 524, 612, 630, 660
GlaI GCGC 1 cut(s) 44
GluI GCNGC 9 cut(s) 87, 281, 284, 287, 290, 323, 401, 767, 780
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 131
HgaI GACGC 2 cut(s) 371, 710
HhaI GCGC 1 cut(s) 45
Hin1II CATG 4 cut(s) 100, 153, 427, 545
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HinfI GANTC 3 cut(s) 20, 254, 527
HpaII CCGG 1 cut(s) 131
HphI GGTGA 1 cut(s) 653
Hpy166II GTNNAC 1 cut(s) 665
Hpy188I TCNGA 4 cut(s) 253, 259, 361, 792
Hpy188III TCNNGA 4 cut(s) 97, 418, 542, 803
Hpy8I GTNNAC 1 cut(s) 665
HpyAV CCTTC 1 cut(s) 96
HpyCH4V TGCA 3 cut(s) 86, 280, 796
HpyF10VI GCNNNNNNNGC 8 cut(s) 61, 286, 289, 388, 397, 406, 621, 630
HpyF3I CTNAG 2 cut(s) 90, 318
Hsp92II CATG 4 cut(s) 100, 153, 427, 545
HspAI GCGC 1 cut(s) 43
KpnI GGTACC 2 cut(s) 77, 109
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 259, 310, 643
LguI GCTCTTC 2 cut(s) 481, 540
LmnI GCTCC 3 cut(s) 61, 517, 763
Lsp1109I GCAGC 7 cut(s) 98, 292, 295, 298, 301, 334, 766
LweI GCATC 3 cut(s) 129, 418, 783
MaeI CTAG 5 cut(s) 240, 524, 612, 630, 660
MaeIII GTNAC 1 cut(s) 652
MalI GATC 3 cut(s) 261, 312, 645
MbiI CCGCTC 1 cut(s) 766
MboI GATC 3 cut(s) 259, 310, 643
MboII GAAGA 5 cut(s) 201, 470, 498, 557, 567
MhlI GDGCHC 2 cut(s) 393, 712
MluCI AATT 5 cut(s) 78, 174, 247, 413, 725
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 2 cut(s) 375, 476
MnlI CCTC 3 cut(s) 64, 85, 363
MseI TTAA 3 cut(s) 162, 234, 405
MspA1I CMGCKG 1 cut(s) 89
MspCI CTTAAG 1 cut(s) 404
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 3 cut(s) 48, 112, 186
MvaI CCWGG 3 cut(s) 48, 112, 186
MwoI GCNNNNNNNGC 8 cut(s) 61, 286, 289, 388, 397, 406, 621, 630
NcoI CCATGG 1 cut(s) 149
NdeII GATC 3 cut(s) 259, 310, 643
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 4 cut(s) 100, 153, 427, 545
NlaIV GGNNCC 4 cut(s) 7, 75, 107, 672
NmeAIII GCCGAG 1 cut(s) 106
NspI RCATGY 1 cut(s) 427
PagI TCATGA 2 cut(s) 96, 541
PciI ACATGT 1 cut(s) 423
PciSI GCTCTTC 2 cut(s) 481, 540
PcsI WCGNNNNNNNCGW 1 cut(s) 718
PfeI GAWTC 2 cut(s) 20, 254
PkrI GCNGC 9 cut(s) 88, 282, 285, 288, 291, 324, 402, 768, 781
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
PscI ACATGT 1 cut(s) 423
Psp6I CCWGG 3 cut(s) 46, 110, 184
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 3 cut(s) 46, 110, 184
PspN4I GGNNCC 4 cut(s) 7, 75, 107, 672
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 365
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 75, 107, 483
RsaNI GTAC 3 cut(s) 74, 106, 482
SapI GCTCTTC 2 cut(s) 481, 540
SaqAI TTAA 3 cut(s) 162, 234, 405
SatI GCNGC 9 cut(s) 87, 281, 284, 287, 290, 323, 401, 767, 780
Sau3AI GATC 3 cut(s) 259, 310, 643
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 3 cut(s) 48, 112, 186
SduI GDGCHC 2 cut(s) 393, 712
SfaNI GCATC 3 cut(s) 129, 418, 783
SfcI CTRYAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SpeI ACTAGT 1 cut(s) 659
Sse9I AATT 5 cut(s) 78, 174, 247, 413, 725
SsiI CCGC 3 cut(s) 214, 400, 766
SspMI CTAG 5 cut(s) 240, 524, 612, 630, 660
StyD4I CCNGG 3 cut(s) 46, 110, 184
StyI CCWWGG 2 cut(s) 149, 774
TasI AATT 5 cut(s) 78, 174, 247, 413, 725
TauI GCSGC 2 cut(s) 403, 769
TfiI GAWTC 2 cut(s) 20, 254
Tru1I TTAA 3 cut(s) 162, 234, 405
Tru9I TTAA 3 cut(s) 162, 234, 405
TscAI CASTG 2 cut(s) 522, 684
TseI GCWGC 7 cut(s) 86, 280, 283, 286, 289, 322, 779
TspDTI ATGAA 1 cut(s) 558
TspRI CASTG 2 cut(s) 522, 684
Vha464I CTTAAG 1 cut(s) 404
XapI RAATTY 1 cut(s) 247
XceI RCATGY 1 cut(s) 427
XmiI GTMKAC 1 cut(s) 664
XspI CTAG 5 cut(s) 240, 524, 612, 630, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.