MD10G1164400.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0017600
Physical Location & Seq
Forward (+)
25524270 .. 25525061
792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1164400.v1.1.491

Sequence Viewer

Length: 792 bp
ATGGCGATGGGAAGCATAGTGAGGGAGGAAGGCGTGCTGAAACTGGTGCGTCCAGGAAGGCGTTTGGAGGTGCACAAGAAACCGGTGACAGCTGAAGAGGTGATGAGAAGGTACCCCAGGCACAGCATAACTAGGCCAGACATCTTTGAGTTCCCATGGATTGTGGTTAAGCCAGAAGCAGTTTTGGTTCCTGGGAAAGTGTTTTTTATAGTTCCTAACCGCACAATTTATCAGTTGCTTAAAGCCAGAGCGCACAGTGATCACCTTCCTTCTTCTTCTTTGCTTGAACAGAAACAATCTGCGGAGGATCGAAGCCACCATCATGTATCTGAACACAGTTCTCCCCCCAAGGACTCTGCTGGTATGACACGGAAGCACAATGAGCAGAGACGAAGGCTTAAGCTTCAATTTTTTGCTTGTGTTACTGCTAGTGTTAGCCCTAGCCCAAGCGATGCTGACTCTGACAGAAGACTCCAAAAAGATATCCAAGTAGAGTTGTCATCCAGAAACAAGAAAACTCGGGGAGAATCTAAGCAGAAACCTCCGATTCATCCAAAAAATGAAACTAGACATCGTAGAAAATACGATGATGGCAACAAGGACATTGCTGCAAATTTTGCTGATCTTAATATTAGGGAGAGCAAGCAGGTCCCCTTGTTGAAATCTTGTATGAGAAAACCTGAAAGTGCTCGCAAGTTGCTTCGCCTTAAGGTAACTCTTAACTTGCCAAACAAGGAAGAGGAGCGACAAAGCGAGGCTACTGATTCTGGGGTGCAATTTCGAGGAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

264

Amino Acids

30.22

Weight (kDa)

10.08

Isoelectric Point (pI)

68.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 9 - 169 1.4e-12 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19050
malus_domestica MD05G1175300.v1.1 MD10G1164400.v1.1
prunus_persica Prupe.8G203000_v2.0.a1
rosa_laevigata RLG00000012706
rosa_multiflora Rmu_sc0000686.1_g000018
rosa_roxburghii Rroxscaffold_7G00184280
rosa_rugosa Rorug06G0167600
rosa_samantha Rh6AG280400 Rh6BG280700 Rh6CG282700 Rh6DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 637
Acc65I GGTACC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 111
AciI CCGC 2 cut(s) 220, 302
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 1 cut(s) 613
AcuI CTGAAG 1 cut(s) 114
AfaI GTAC 1 cut(s) 113
AflII CTTAAG 2 cut(s) 398, 707
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 3 cut(s) 287, 407, 661
AjnI CCWGG 3 cut(s) 52, 116, 190
AluBI AGCT 2 cut(s) 92, 403
AluI AGCT 2 cut(s) 92, 403
Alw21I GWGCWC 2 cut(s) 75, 691
Alw26I GTCTC 1 cut(s) 382
Alw44I GTGCAC 1 cut(s) 71
AlwI GGATC 1 cut(s) 315
Ama87I CYCGRG 1 cut(s) 519
AoxI GGCC 1 cut(s) 134
ApaLI GTGCAC 1 cut(s) 71
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 1 cut(s) 613
AsiGI ACCGGT 1 cut(s) 82
Asp718I GGTACC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 649
AsuHPI GGTGA 3 cut(s) 97, 112, 254
AvaI CYCGRG 1 cut(s) 519
AvaII GGWCC 1 cut(s) 649
BaeGI GKGCMC 1 cut(s) 75
BanI GGYRCC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 475
Bbv12I GWGCWC 2 cut(s) 75, 691
BbvI GCAGC 1 cut(s) 595
BccI CCATC 2 cut(s) 327, 584
BciT130I CCWGG 3 cut(s) 54, 118, 192
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 4 cut(s) 132, 429, 441, 567
BfrI CTTAAG 2 cut(s) 398, 707
BfuAI ACCTGC 1 cut(s) 637
BisI GCNGC 1 cut(s) 609
BlsI GCNGC 1 cut(s) 610
Bme1390I CCNGG 3 cut(s) 54, 118, 192
Bme18I GGWCC 1 cut(s) 649
BmeT110I CYCGRG 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 649
BmiI GGNNCC 3 cut(s) 113, 189, 651
BmrFI CCNGG 3 cut(s) 54, 118, 192
BmsI GCATC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 475
BsaJI CCNNGG 4 cut(s) 116, 155, 191, 348
BsaWI WCCGGW 1 cut(s) 82
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 3 cut(s) 54, 118, 192
BseDI CCNNGG 4 cut(s) 116, 155, 191, 348
BseGI GGATG 2 cut(s) 500, 550
BseMI GCAATG 1 cut(s) 603
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 1 cut(s) 755
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 1 cut(s) 595
BshFI GGCC 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 111
BshTI ACCGGT 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 75, 691
BsiHKCI CYCGRG 1 cut(s) 519
BsiSI CCGG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 635
BsmAI GTCTC 1 cut(s) 382
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 1 cut(s) 635
BsnI GGCC 1 cut(s) 136
BsoBI CYCGRG 1 cut(s) 519
Bsp1286I GDGCHC 2 cut(s) 75, 691
Bsp143I GATC 3 cut(s) 259, 307, 622
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 2 cut(s) 220, 302
BspANI GGCC 1 cut(s) 136
BspLI GGNNCC 3 cut(s) 113, 189, 651
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 111
BspTI CTTAAG 2 cut(s) 398, 707
BsrDI GCAATG 1 cut(s) 603
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 4 cut(s) 116, 155, 191, 348
BssMI GATC 3 cut(s) 259, 307, 622
BssT1I CCWWGG 2 cut(s) 155, 348
Bst2UI CCWGG 3 cut(s) 54, 118, 192
Bst4CI ACNGT 2 cut(s) 257, 338
Bst6I CTCTTC 2 cut(s) 90, 732
BstAFI CTTAAG 2 cut(s) 398, 707
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 3 cut(s) 35, 644, 691
BstDEI CTNAG 1 cut(s) 531
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 500, 550
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 3 cut(s) 262, 310, 625
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 3 cut(s) 259, 307, 622
BstMWI GCNNNNNNNGC 2 cut(s) 382, 617
BstNI CCWGG 3 cut(s) 54, 118, 192
BstSCI CCNGG 3 cut(s) 52, 116, 190
BstSLI GKGCMC 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 595
BstV2I GAAGAC 1 cut(s) 475
BsuRI GGCC 1 cut(s) 136
BtgI CCRYGG 1 cut(s) 155
BtgZI GCGATG 2 cut(s) 20, 465
BtsCI GGATG 2 cut(s) 500, 550
BtsIMutI CAGTG 1 cut(s) 262
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 3 cut(s) 35, 644, 691
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 649
CseI GACGC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 112
CspAI ACCGGT 1 cut(s) 82
CviAII CATG 2 cut(s) 156, 323
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 1 cut(s) 531
DpnI GATC 3 cut(s) 261, 309, 624
DpnII GATC 3 cut(s) 259, 307, 622
Eam1104I CTCTTC 2 cut(s) 90, 732
EarI CTCTTC 2 cut(s) 90, 732
Eco130I CCWWGG 2 cut(s) 155, 348
Eco32I GATATC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 649
Eco57I CTGAAG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 649
EcoRII CCWGG 3 cut(s) 52, 116, 190
EcoRV GATATC 1 cut(s) 484
EcoT14I CCWWGG 2 cut(s) 155, 348
ErhI CCWWGG 2 cut(s) 155, 348
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 2 cut(s) 159, 326
FaiI YATR 7 cut(s) 17, 128, 157, 209, 324, 365, 671
FaqI GGGAC 1 cut(s) 635
FatI CATG 2 cut(s) 155, 322
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 1 cut(s) 609
FokI GGATG 2 cut(s) 487, 537
Fsp4HI GCNGC 1 cut(s) 609
FspBI CTAG 4 cut(s) 132, 429, 441, 567
GlaI GCGC 1 cut(s) 252
GluI GCNGC 1 cut(s) 609
HaeIII GGCC 1 cut(s) 136
HapII CCGG 1 cut(s) 83
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 253
Hin1II CATG 2 cut(s) 159, 326
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 6 cut(s) 353, 458, 471, 527, 547, 764
HpaII CCGG 1 cut(s) 83
HphI GGTGA 3 cut(s) 97, 112, 254
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 331, 463, 546
Hpy188III TCNNGA 1 cut(s) 504
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 6 cut(s) 23, 51, 102, 275, 279, 387
HpyCH4III ACNGT 2 cut(s) 257, 338
HpyCH4V TGCA 3 cut(s) 73, 611, 775
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 617
HpyF3I CTNAG 1 cut(s) 531
Hsp92II CATG 2 cut(s) 159, 326
HspAI GCGC 1 cut(s) 251
KpnI GGTACC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 3 cut(s) 259, 307, 622
LmnI GCTCC 1 cut(s) 742
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 1 cut(s) 442
MaeI CTAG 4 cut(s) 132, 429, 441, 567
MaeIII GTNAC 3 cut(s) 85, 421, 712
MalI GATC 3 cut(s) 261, 309, 624
MboI GATC 3 cut(s) 259, 307, 622
MboII GAAGA 5 cut(s) 107, 264, 267, 480, 749
MhlI GDGCHC 2 cut(s) 75, 691
MluCI AATT 4 cut(s) 225, 407, 613, 776
MlyI GAGTC 3 cut(s) 347, 452, 465
MnlI CCTC 9 cut(s) 15, 19, 61, 91, 298, 552, 733, 748, 776
MseI TTAA 6 cut(s) 168, 240, 399, 627, 708, 720
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 92
MspCI CTTAAG 2 cut(s) 398, 707
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 3 cut(s) 54, 118, 192
MvaI CCWGG 3 cut(s) 54, 118, 192
MwoI GCNNNNNNNGC 2 cut(s) 382, 617
NcoI CCATGG 1 cut(s) 155
NdeII GATC 3 cut(s) 259, 307, 622
NlaIII CATG 2 cut(s) 159, 326
NlaIV GGNNCC 3 cut(s) 113, 189, 651
NmuCI GTSAC 1 cut(s) 85
PfeI GAWTC 3 cut(s) 527, 547, 764
PfoI TCCNGGA 1 cut(s) 52
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 1 cut(s) 610
PleI GAGTC 3 cut(s) 347, 452, 465
PpsI GAGTC 3 cut(s) 347, 452, 465
PpuMI RGGWCCY 1 cut(s) 649
Psp5II RGGWCCY 1 cut(s) 649
Psp6I CCWGG 3 cut(s) 52, 116, 190
PspGI CCWGG 3 cut(s) 52, 116, 190
PspN4I GGNNCC 3 cut(s) 113, 189, 651
PspPI GGNCC 1 cut(s) 649
PspPPI RGGWCCY 1 cut(s) 649
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 6 cut(s) 168, 240, 399, 627, 708, 720
SatI GCNGC 1 cut(s) 609
Sau3AI GATC 3 cut(s) 259, 307, 622
Sau96I GGNCC 1 cut(s) 649
SchI GAGTC 3 cut(s) 347, 452, 465
ScrFI CCNGG 3 cut(s) 54, 118, 192
SduI GDGCHC 2 cut(s) 75, 691
SfaNI GCATC 1 cut(s) 442
SinI GGWCC 1 cut(s) 649
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 398, 707
SmoI CTYRAG 2 cut(s) 398, 707
Sse9I AATT 4 cut(s) 225, 407, 613, 776
SsiI CCGC 2 cut(s) 220, 302
SspI AATATT 1 cut(s) 631
SspMI CTAG 4 cut(s) 132, 429, 441, 567
StyD4I CCNGG 3 cut(s) 52, 116, 190
StyI CCWWGG 2 cut(s) 155, 348
TaaI ACNGT 2 cut(s) 257, 338
TaqI TCGA 2 cut(s) 310, 781
TasI AATT 4 cut(s) 225, 407, 613, 776
TfiI GAWTC 3 cut(s) 527, 547, 764
Tru1I TTAA 6 cut(s) 168, 240, 399, 627, 708, 720
Tru9I TTAA 6 cut(s) 168, 240, 399, 627, 708, 720
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 2 cut(s) 539, 576
TspGWI ACGGA 1 cut(s) 385
TspRI CASTG 1 cut(s) 262
Vha464I CTTAAG 2 cut(s) 398, 707
VneI GTGCAC 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 649
XapI RAATTY 1 cut(s) 613
XspI CTAG 4 cut(s) 132, 429, 441, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.