RLG00000012706

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017600
Physical Location & Seq
Reverse (-)
21496117 .. 21496950
834 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012706

Sequence Viewer

Length: 834 bp
ATGGGAACCATAGCAAAGGAAGCAGGTGTGCTGAAACTCGTGCGCCCTGGTAGGCGAGTGGAGCTTCATCGGGTACCAATTACTGCAGCTGAGGTCATGAGAAGGTACCCGAGGCACAGCATCACTCGCCCCGATATCTTCGAGTTCCCATGGATTGTGGTTAAGCCAGAAGCAGTTTTGACCCCTGGAACCGCTGGAAGAGTGTTCTTCATAGTTCCTAATCGCACCATCTATCAGTTGCTTAAAGCTAGAGAGAACTCTGATTCTGATCAGTCTTCACCTTCTGCTTCGCAACAGAAACAACAAGAGTCTCCGAAAGATCAAGCTCAACAAGAAGTATTGAAAAAGACCTTCCCACCCAACAGGACAGATGCTGGGATGACACCAAAGCACCGAGAGCACAGGCGGCGGCTTAAGCATCAGTTCTTGAACATGTCTTGTGCTAGTACTGTGTGTCCTACTGAAGAACAAGACTATGACAAAGAAGTACGAAGAGATGTACAAAGGGTGTCTGCGCCGGTGTTGCTAGGCCACAAGACAGCTCAGGGAGAACCTAAACGAAAATCATCATCTCTATCTTCAAAAAATGAGACTAGAAAAGGTAAAGGTAAAGCTGGCCTCAGTAGCATTACTGGCAGTAAACTTGGTGATCTCAAGTTTACAACCACTAAACAGGTCCCCGCACCGAAATCTTGTATAAGAAAACCACATAGTGCTCGTAAGTTGCTTCAACTTAGAGTGAAATTTGACTTGCCAGACGACTATGCGGAGCGGCAAACCAAGGCCGCTGATTGTCGGATGCATCGGATGCAATATCCAGACTATAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

278

Amino Acids

31.47

Weight (kDa)

10.13

Isoelectric Point (pI)

55.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 167 3.1e-12 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19050
malus_domestica MD05G1175300.v1.1 MD10G1164400.v1.1
prunus_persica Prupe.8G203000_v2.0.a1
rosa_laevigata RLG00000012706
rosa_multiflora Rmu_sc0000686.1_g000018
rosa_roxburghii Rroxscaffold_7G00184280
rosa_rugosa Rorug06G0167600
rosa_samantha Rh6AG280400 Rh6BG280700 Rh6CG282700 Rh6DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 14
Acc65I GGTACC 2 cut(s) 73, 105
AccB1I GGYRCC 2 cut(s) 73, 105
AccBSI CCGCTC 1 cut(s) 772
AciI CCGC 7 cut(s) 192, 406, 409, 681, 767, 772, 786
AcsI RAATTY 1 cut(s) 743
AcuI CTGAAG 1 cut(s) 483
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 5 cut(s) 75, 107, 448, 489, 501
AflII CTTAAG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 4 cut(s) 343, 430, 582, 731
AjnI CCWGG 2 cut(s) 46, 184
AluBI AGCT 6 cut(s) 64, 89, 248, 326, 542, 614
AluI AGCT 6 cut(s) 64, 89, 248, 326, 542, 614
Alw21I GWGCWC 2 cut(s) 402, 718
Alw26I GTCTC 2 cut(s) 315, 584
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 3 cut(s) 529, 616, 783
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 743
Asp718I GGTACC 2 cut(s) 73, 105
AspLEI GCGC 2 cut(s) 45, 517
AspS9I GGNCC 1 cut(s) 676
AsuHPI GGTGA 2 cut(s) 270, 659
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 1 cut(s) 676
BanI GGYRCC 2 cut(s) 73, 105
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 2 cut(s) 402, 718
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 98
BccI CCATC 1 cut(s) 236
BciT130I CCWGG 2 cut(s) 48, 186
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 315, 584
BfaI CTAG 4 cut(s) 249, 444, 527, 594
BfmI CTRYAG 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 413
BfuAI ACCTGC 1 cut(s) 14
BisI GCNGC 5 cut(s) 87, 407, 410, 773, 786
BlsI GCNGC 5 cut(s) 88, 408, 411, 774, 787
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 48, 186
Bme18I GGWCC 1 cut(s) 676
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 676
BmiI GGNNCC 5 cut(s) 7, 75, 107, 190, 678
BmrFI CCNGG 2 cut(s) 48, 186
BmsI GCATC 6 cut(s) 129, 361, 427, 789, 798, 811
BpiI GAAGAC 1 cut(s) 267
Bpu10I CCTNAGC 2 cut(s) 90, 543
BpuEI CTTGAG 1 cut(s) 638
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 5 cut(s) 46, 110, 149, 184, 780
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 1 cut(s) 637
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 48, 186
BseDI CCNNGG 5 cut(s) 46, 110, 149, 184, 780
BseGI GGATG 3 cut(s) 384, 804, 813
BseJI GATNNNNATC 1 cut(s) 267
BseMII CTCAG 3 cut(s) 81, 557, 634
BseNI ACTGG 1 cut(s) 637
BseXI GCAGC 1 cut(s) 98
BseYI CCCAGC 1 cut(s) 374
BshFI GGCC 3 cut(s) 531, 618, 785
BshNI GGYRCC 2 cut(s) 73, 105
BsiHKAI GWGCWC 2 cut(s) 402, 718
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 1 cut(s) 662
BsmAI GTCTC 2 cut(s) 315, 584
BsmFI GGGAC 1 cut(s) 662
BsnI GGCC 3 cut(s) 531, 618, 785
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 402, 718
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 3 cut(s) 268, 319, 649
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 7 cut(s) 192, 406, 409, 681, 767, 772, 786
BspANI GGCC 3 cut(s) 531, 618, 785
BspCNI CTCAG 3 cut(s) 82, 556, 633
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 5 cut(s) 7, 75, 107, 190, 678
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 14
BspT107I GGYRCC 2 cut(s) 73, 105
BspTI CTTAAG 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 772
BsrFI RCCGGY 1 cut(s) 517
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 1 cut(s) 637
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 5 cut(s) 46, 110, 149, 184, 780
BssMI GATC 3 cut(s) 268, 319, 649
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 149, 780
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 2 cut(s) 48, 186
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 2 cut(s) 193, 487
BstAFI CTTAAG 1 cut(s) 413
BstAPI GCANNNNNTGC 1 cut(s) 808
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 1 cut(s) 616
BstDEI CTNAG 4 cut(s) 90, 543, 620, 734
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 3 cut(s) 384, 804, 813
BstHHI GCGC 2 cut(s) 45, 517
BstKTI GATC 3 cut(s) 271, 322, 652
BstMAI GTCTC 2 cut(s) 315, 584
BstMBI GATC 3 cut(s) 268, 319, 649
BstNI CCWGG 2 cut(s) 48, 186
BstNSI RCATGY 1 cut(s) 436
BstSCI CCNGG 2 cut(s) 46, 184
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 267
BsuRI GGCC 3 cut(s) 531, 618, 785
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 3 cut(s) 384, 804, 813
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 616
CaiI CAGNNNCTG 1 cut(s) 374
CciI TCATGA 1 cut(s) 96
CfoI GCGC 2 cut(s) 45, 517
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 676
Csp6I GTAC 5 cut(s) 74, 106, 447, 488, 500
CviAII CATG 3 cut(s) 97, 150, 433
CviQI GTAC 5 cut(s) 74, 106, 447, 488, 500
DdeI CTNAG 4 cut(s) 90, 543, 620, 734
DpnI GATC 3 cut(s) 270, 321, 651
DpnII GATC 3 cut(s) 268, 319, 649
DraIII CACNNNGTG 1 cut(s) 713
Eam1104I CTCTTC 2 cut(s) 193, 487
EarI CTCTTC 2 cut(s) 193, 487
Eco130I CCWWGG 2 cut(s) 149, 780
Eco32I GATATC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 676
Eco57I CTGAAG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 46, 184
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 149, 780
EcoT22I ATGCAT 1 cut(s) 804
ErhI CCWWGG 2 cut(s) 149, 780
FaeI CATG 3 cut(s) 100, 153, 436
FaqI GGGAC 1 cut(s) 662
FatI CATG 3 cut(s) 96, 149, 432
FauI CCCGC 1 cut(s) 688
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 5 cut(s) 87, 407, 410, 773, 786
FokI GGATG 3 cut(s) 391, 811, 820
Fsp4HI GCNGC 5 cut(s) 87, 407, 410, 773, 786
FspBI CTAG 4 cut(s) 249, 444, 527, 594
GlaI GCGC 2 cut(s) 44, 516
GluI GCNGC 5 cut(s) 87, 407, 410, 773, 786
GsaI CCCAGC 1 cut(s) 378
HaeIII GGCC 3 cut(s) 531, 618, 785
HapII CCGG 1 cut(s) 518
HhaI GCGC 2 cut(s) 45, 517
Hin1II CATG 3 cut(s) 100, 153, 436
Hin6I GCGC 2 cut(s) 43, 515
HinP1I GCGC 2 cut(s) 43, 515
HinfI GANTC 2 cut(s) 263, 308
HpaII CCGG 1 cut(s) 518
HphI GGTGA 2 cut(s) 270, 659
Hpy166II GTNNAC 2 cut(s) 641, 660
Hpy188I TCNGA 5 cut(s) 262, 268, 315, 798, 807
Hpy188III TCNNGA 3 cut(s) 97, 427, 818
Hpy8I GTNNAC 2 cut(s) 641, 660
HpyAV CCTTC 3 cut(s) 96, 291, 361
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4V TGCA 3 cut(s) 86, 802, 811
HpyF3I CTNAG 4 cut(s) 90, 543, 620, 734
Hsp92II CATG 3 cut(s) 100, 153, 436
HspAI GCGC 2 cut(s) 43, 515
KpnI GGTACC 2 cut(s) 77, 109
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 268, 319, 649
LmnI GCTCC 2 cut(s) 61, 769
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 6 cut(s) 129, 361, 427, 789, 798, 811
MaeI CTAG 4 cut(s) 249, 444, 527, 594
MalI GATC 3 cut(s) 270, 321, 651
MbiI CCGCTC 1 cut(s) 772
MboI GATC 3 cut(s) 268, 319, 649
MboII GAAGA 7 cut(s) 130, 199, 210, 267, 476, 504, 570
MhlI GDGCHC 2 cut(s) 402, 718
MluCI AATT 2 cut(s) 78, 743
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 776
MnlI CCTC 3 cut(s) 85, 105, 629
Mph1103I ATGCAT 1 cut(s) 804
MseI TTAA 3 cut(s) 162, 243, 414
MspA1I CMGCKG 3 cut(s) 89, 194, 788
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 2 cut(s) 48, 186
MvaI CCWGG 2 cut(s) 48, 186
NcoI CCATGG 1 cut(s) 149
NdeII GATC 3 cut(s) 268, 319, 649
NlaIII CATG 3 cut(s) 100, 153, 436
NlaIV GGNNCC 5 cut(s) 7, 75, 107, 190, 678
NsiI ATGCAT 1 cut(s) 804
NspI RCATGY 1 cut(s) 436
PagI TCATGA 1 cut(s) 96
PaqCI CACCTGC 1 cut(s) 14
PciI ACATGT 1 cut(s) 432
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 5 cut(s) 88, 408, 411, 774, 787
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 676
PscI ACATGT 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 2 cut(s) 46, 184
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 2 cut(s) 46, 184
PspN4I GGNNCC 5 cut(s) 7, 75, 107, 190, 678
PspPI GGNCC 1 cut(s) 676
PspPPI RGGWCCY 1 cut(s) 676
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 5 cut(s) 75, 107, 448, 489, 501
RsaNI GTAC 5 cut(s) 74, 106, 447, 488, 500
SaqAI TTAA 3 cut(s) 162, 243, 414
SatI GCNGC 5 cut(s) 87, 407, 410, 773, 786
Sau3AI GATC 3 cut(s) 268, 319, 649
Sau96I GGNCC 1 cut(s) 676
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 2 cut(s) 48, 186
SduI GDGCHC 2 cut(s) 402, 718
SfaNI GCATC 6 cut(s) 129, 361, 427, 789, 798, 811
SfcI CTRYAG 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 517
SinI GGWCC 1 cut(s) 676
SmlI CTYRAG 2 cut(s) 413, 653
SmoI CTYRAG 2 cut(s) 413, 653
Sse9I AATT 2 cut(s) 78, 743
SsiI CCGC 7 cut(s) 192, 406, 409, 681, 767, 772, 786
SspMI CTAG 4 cut(s) 249, 444, 527, 594
StyD4I CCNGG 2 cut(s) 46, 184
StyI CCWWGG 2 cut(s) 149, 780
TaaI ACNGT 1 cut(s) 451
TaqI TCGA 1 cut(s) 141
TasI AATT 2 cut(s) 78, 743
TatI WGTACW 2 cut(s) 446, 499
TauI GCSGC 4 cut(s) 409, 412, 775, 788
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 162, 243, 414
Tru9I TTAA 3 cut(s) 162, 243, 414
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 56, 199
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 676
XapI RAATTY 1 cut(s) 743
XceI RCATGY 1 cut(s) 436
XspI CTAG 4 cut(s) 249, 444, 527, 594
ZrmI AGTACT 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.