Prupe.8G203000_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0017600
Physical Location & Seq
Forward (+)
19106635 .. 19107913
1279 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G203000.1

Sequence Viewer

Length: 756 bp
ATGGGAAGCATAACAAGGGAGGAAGGTGTGCTCAAGTTGGTGCGTCCGGGGAGGCGTGTGGAGGTTCACAAGGAACCAATAACAGCTCAAGAGGTGATGAGAAAGTACCCCAGGCACTCCATAACTAGGCCAGACATCTTTGAGTTCCCATGGATTGTGGTTAAGGCTGAAGCAGTTTTGACTCCTGGAAAAGTGTTTTTTATAGTTCCTAACCGTACGATTTATCAGCTGCTTAAAGCCCGAGAGCACGGTTACCAATCTTCTTTTTCTTCTTCCTCACAGCAGCAGAGACAATCTGCTAGAGATCATGGTCACCATCCAGCATCAGAACAACTAACTTCCCCACCAAAGGCATATGCTGGCGTGACACTGAAGCATCAAGAGCGGAGACGAAGGCTTAAGCATCAGTTCTTTTCTTGTGCTACTGTAAGCCCTAGTGATGAAGAAGTTGACTCGGACAAAGGGCTCCAAACAGATTCCCAGGTTGGAGCTTGGCCAGGGTTGTCTTCCACAAACAAGAAAACTCACGGAAAATCTAAACAGAAGCCACAGATAAATTCAAAAATTAAAACTAGATATGGTAGAGTATATGACTACAGCAAGAAGAATAACATTACGGCCAAACTTGCTGGTCTTGAGATTACAGATAGTAAACAGGTCACCTTGTTGAAATCCTGTTTAAGAAAACCCGATAGTGCTCGTAAGTCTCTTCGTCTTAAGGTAGCTTTTACCTTGCCAAACAAGGACGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

252

Amino Acids

28.52

Weight (kDa)

10.08

Isoelectric Point (pI)

53.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19050
malus_domestica MD05G1175300.v1.1 MD10G1164400.v1.1
prunus_persica Prupe.8G203000_v2.0.a1
rosa_laevigata RLG00000012706
rosa_multiflora Rmu_sc0000686.1_g000018
rosa_roxburghii Rroxscaffold_7G00184280
rosa_rugosa Rorug06G0167600
rosa_samantha Rh6AG280400 Rh6BG280700 Rh6CG282700 Rh6DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 385
AciI CCGC 1 cut(s) 385
AcoI YGGCCR 2 cut(s) 494, 618
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 2 cut(s) 189, 392
AfaI GTAC 2 cut(s) 107, 217
AfiI CCNNNNNNNGG 2 cut(s) 126, 349
AflII CTTAAG 2 cut(s) 398, 716
AgsI TTSAA 2 cut(s) 561, 670
AjnI CCWGG 4 cut(s) 110, 184, 480, 496
AluBI AGCT 4 cut(s) 86, 229, 491, 725
AluI AGCT 4 cut(s) 86, 229, 491, 725
Alw21I GWGCWC 3 cut(s) 33, 249, 700
Alw26I GTCTC 3 cut(s) 283, 382, 711
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 3 cut(s) 128, 494, 618
ApeKI GCWGC 2 cut(s) 229, 283
ApoI RAATTY 1 cut(s) 556
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 3 cut(s) 106, 305, 652
AvaI CYCGRG 1 cut(s) 240
BalI TGGCCA 1 cut(s) 496
BanII GRGCYC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 498
Bbv12I GWGCWC 3 cut(s) 33, 249, 700
BbvI GCAGC 2 cut(s) 216, 295
BccI CCATC 1 cut(s) 324
BceAI ACGGC 1 cut(s) 633
BciT130I CCWGG 4 cut(s) 112, 186, 482, 498
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 3 cut(s) 283, 382, 711
BfaI CTAG 4 cut(s) 126, 300, 435, 573
BfmI CTRYAG 1 cut(s) 595
BfrI CTTAAG 2 cut(s) 398, 716
BisI GCNGC 2 cut(s) 230, 284
BlsI GCNGC 2 cut(s) 231, 285
Bme1390I CCNGG 5 cut(s) 48, 112, 186, 482, 498
BmeT110I CYCGRG 1 cut(s) 240
BmiI GGNNCC 2 cut(s) 75, 467
BmrFI CCNGG 5 cut(s) 48, 112, 186, 482, 498
BmsI GCATC 3 cut(s) 332, 385, 412
BpiI GAAGAC 1 cut(s) 498
BpuEI CTTGAG 3 cut(s) 17, 72, 656
BpuMI CCSGG 1 cut(s) 48
BsaJI CCNNGG 5 cut(s) 47, 110, 149, 480, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 349
BseBI CCWGG 4 cut(s) 112, 186, 482, 498
BseDI CCNNGG 5 cut(s) 47, 110, 149, 480, 497
BseGI GGATG 1 cut(s) 316
BseLI CCNNNNNNNGG 2 cut(s) 126, 349
BseXI GCAGC 2 cut(s) 216, 295
BshFI GGCC 3 cut(s) 130, 496, 620
BsiHKAI GWGCWC 3 cut(s) 33, 249, 700
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 1 cut(s) 47
BsiWI CGTACG 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 126, 349
BsmAI GTCTC 3 cut(s) 283, 382, 711
BsmBI CGTCTC 1 cut(s) 382
BsnI GGCC 3 cut(s) 130, 496, 620
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 4 cut(s) 33, 249, 468, 700
Bsp143I GATC 1 cut(s) 304
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 1 cut(s) 385
BspANI GGCC 3 cut(s) 130, 496, 620
BspLI GGNNCC 2 cut(s) 75, 467
BspTI CTTAAG 2 cut(s) 398, 716
BsrBI CCGCTC 1 cut(s) 385
BssECI CCNNGG 5 cut(s) 47, 110, 149, 480, 497
BssMI GATC 1 cut(s) 304
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 4 cut(s) 112, 186, 482, 498
Bst4CI ACNGT 3 cut(s) 215, 251, 427
Bst6I CTCTTC 1 cut(s) 714
BstAFI CTTAAG 2 cut(s) 398, 716
BstC8I GCNNGC 1 cut(s) 361
BstDSI CCRYGG 1 cut(s) 149
BstEII GGTNACC 3 cut(s) 251, 311, 658
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 1 cut(s) 307
BstMAI GTCTC 3 cut(s) 283, 382, 711
BstMBI GATC 1 cut(s) 304
BstMWI GCNNNNNNNGC 2 cut(s) 382, 626
BstNI CCWGG 4 cut(s) 112, 186, 482, 498
BstPI GGTNACC 3 cut(s) 251, 311, 658
BstSCI CCNGG 5 cut(s) 46, 110, 184, 480, 496
BstSFI CTRYAG 1 cut(s) 595
BstV1I GCAGC 2 cut(s) 216, 295
BstV2I GAAGAC 1 cut(s) 498
BsuRI GGCC 3 cut(s) 130, 496, 620
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 368
Cac8I GCNNGC 1 cut(s) 361
CseI GACGC 1 cut(s) 32
Csp6I GTAC 2 cut(s) 106, 216
CviAII CATG 2 cut(s) 150, 308
CviQI GTAC 2 cut(s) 106, 216
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
EaeI YGGCCR 2 cut(s) 494, 618
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco130I CCWWGG 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 468
Eco57I CTGAAG 2 cut(s) 189, 392
Eco88I CYCGRG 1 cut(s) 240
Eco91I GGTNACC 3 cut(s) 251, 311, 658
EcoO65I GGTNACC 3 cut(s) 251, 311, 658
EcoRII CCWGG 4 cut(s) 110, 184, 480, 496
EcoT14I CCWWGG 1 cut(s) 149
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 382
FaeI CATG 2 cut(s) 153, 311
FatI CATG 2 cut(s) 149, 307
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 2 cut(s) 230, 284
FokI GGATG 1 cut(s) 303
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 2 cut(s) 230, 284
FspBI CTAG 4 cut(s) 126, 300, 435, 573
GluI GCNGC 2 cut(s) 230, 284
HaeIII GGCC 3 cut(s) 130, 496, 620
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 32
Hin1II CATG 2 cut(s) 153, 311
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 3 cut(s) 181, 452, 476
HpaII CCGG 1 cut(s) 47
HphI GGTGA 3 cut(s) 106, 305, 652
Hpy166II GTNNAC 3 cut(s) 67, 451, 653
Hpy188I TCNGA 2 cut(s) 328, 457
Hpy188III TCNNGA 3 cut(s) 89, 380, 635
Hpy8I GTNNAC 3 cut(s) 67, 451, 653
HpyAV CCTTC 2 cut(s) 17, 387
HpyCH4III ACNGT 3 cut(s) 215, 251, 427
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 626
Hsp92II CATG 2 cut(s) 153, 311
Kzo9I GATC 1 cut(s) 304
LmnI GCTCC 2 cut(s) 471, 488
Lsp1109I GCAGC 2 cut(s) 216, 295
LweI GCATC 3 cut(s) 332, 385, 412
MaeI CTAG 4 cut(s) 126, 300, 435, 573
MaeIII GTNAC 4 cut(s) 251, 311, 364, 658
MalI GATC 1 cut(s) 306
MbiI CCGCTC 1 cut(s) 385
MboI GATC 1 cut(s) 304
MboII GAAGA 7 cut(s) 252, 261, 264, 455, 498, 616, 701
MhlI GDGCHC 4 cut(s) 33, 249, 468, 700
MlsI TGGCCA 1 cut(s) 496
MluCI AATT 2 cut(s) 556, 564
MluNI TGGCCA 1 cut(s) 496
MlyI GAGTC 2 cut(s) 175, 446
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 6 cut(s) 13, 45, 55, 85, 286, 742
Mox20I TGGCCA 1 cut(s) 496
MscI TGGCCA 1 cut(s) 496
MseI TTAA 6 cut(s) 162, 234, 399, 567, 680, 717
Msp20I TGGCCA 1 cut(s) 496
MspA1I CMGCKG 1 cut(s) 229
MspCI CTTAAG 2 cut(s) 398, 716
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 5 cut(s) 48, 112, 186, 482, 498
MvaI CCWGG 4 cut(s) 112, 186, 482, 498
MwoI GCNNNNNNNGC 2 cut(s) 382, 626
NciI CCSGG 1 cut(s) 48
NcoI CCATGG 1 cut(s) 149
NdeI CATATG 1 cut(s) 355
NdeII GATC 1 cut(s) 304
NlaIII CATG 2 cut(s) 153, 311
NlaIV GGNNCC 2 cut(s) 75, 467
NmuCI GTSAC 3 cut(s) 311, 364, 658
PfeI GAWTC 1 cut(s) 476
Pfl23II CGTACG 1 cut(s) 215
PfoI TCCNGGA 1 cut(s) 184
PkrI GCNGC 2 cut(s) 231, 285
PleI GAGTC 2 cut(s) 175, 446
PpsI GAGTC 2 cut(s) 175, 446
Psp6I CCWGG 4 cut(s) 110, 184, 480, 496
PspEI GGTNACC 3 cut(s) 251, 311, 658
PspGI CCWGG 4 cut(s) 110, 184, 480, 496
PspLI CGTACG 1 cut(s) 215
PspN4I GGNNCC 2 cut(s) 75, 467
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 2 cut(s) 107, 217
RsaNI GTAC 2 cut(s) 106, 216
SaqAI TTAA 6 cut(s) 162, 234, 399, 567, 680, 717
SatI GCNGC 2 cut(s) 230, 284
Sau3AI GATC 1 cut(s) 304
SchI GAGTC 2 cut(s) 175, 446
ScrFI CCNGG 5 cut(s) 48, 112, 186, 482, 498
SduI GDGCHC 4 cut(s) 33, 249, 468, 700
SfaNI GCATC 3 cut(s) 332, 385, 412
SfcI CTRYAG 1 cut(s) 595
SmlI CTYRAG 5 cut(s) 32, 87, 398, 635, 716
SmoI CTYRAG 5 cut(s) 32, 87, 398, 635, 716
Sse9I AATT 2 cut(s) 556, 564
SsiI CCGC 1 cut(s) 385
SspMI CTAG 4 cut(s) 126, 300, 435, 573
StyD4I CCNGG 5 cut(s) 46, 110, 184, 480, 496
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 3 cut(s) 215, 251, 427
TasI AATT 2 cut(s) 556, 564
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 6 cut(s) 162, 234, 399, 567, 680, 717
Tru9I TTAA 6 cut(s) 162, 234, 399, 567, 680, 717
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 3 cut(s) 311, 364, 658
TseI GCWGC 2 cut(s) 229, 283
Tsp45I GTSAC 3 cut(s) 311, 364, 658
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 1 cut(s) 375
Vha464I CTTAAG 2 cut(s) 398, 716
XapI RAATTY 1 cut(s) 556
XspI CTAG 4 cut(s) 126, 300, 435, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.