Rroxscaffold_7G00184280

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017600
Physical Location & Seq
Reverse (-)
23504502 .. 23505287
786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00184280.1

Sequence Viewer

Length: 786 bp
ATGGGAACCATAGCAAAGGAAGCAGGTGTGCTGAAACTGGTGCGCCCTGGTAGGCGAGTGGAGCTTCATCGGGTACCAATTACTGCAGCTGAGGTCATGAGAAGGTACCCGAGGCACAGCATCACTCGCCCCGATATCTTCGAGTTCCCATGGATTGTGGTTAAGCCAGAAGCAGTTTTGACCCCTGGAACCGCTGGAAGAGTGTTCTTCATAGTTCCTAACCGCACTATCTATCAGTTGCTTAAAGCTAGAGAGAACTCTGATTCTGATCAGTCTTCACCTTCTGCTTCGCAACAGAAACAACAAGAGTCTCCGAAAGATCAAGCTCAACAACAAGAATCGAAAAAGACCTTCCCACCCAACAGGACAGATGCTGGGATGACACCAAAGCACCAAGAGCACAGGCGGCGGCTTAAGCATCAGTTCTTGAACATATCTTGTGCTACTACTACTGTGTGTCCTGCTGAAGAACAAGACTATGACAAAGGAGTACGAAGAAATGTACAAAGGGTGTCTGCGCCGGTGTTGGTAGGCCACAAGACAGCTCAGGCAGAGCCTAAACGAAAATCATCATCTCTATCTTCAAAAAATGAGACTAGAAAAGGTAAAGCTAGCCTCGGCAGCATTACTAGCAAACTTGGTGATCTCAAGTTTACAACCAGTAAACAGGTCCCCGCCCTGAAATCTTGTATAAAAAAACCAGATAGTGCTCGTAAGTGGCGTCAACTTATAGTGAAATTTCACTTGCCAGACGACTATGTAGAGCGGCAAACCAAAGGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

261

Amino Acids

29.51

Weight (kDa)

10.34

Isoelectric Point (pI)

55.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 173 3.1e-12 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19050
malus_domestica MD05G1175300.v1.1 MD10G1164400.v1.1
prunus_persica Prupe.8G203000_v2.0.a1
rosa_laevigata RLG00000012706
rosa_multiflora Rmu_sc0000686.1_g000018
rosa_roxburghii Rroxscaffold_7G00184280
rosa_rugosa Rorug06G0167600
rosa_samantha Rh6AG280400 Rh6BG280700 Rh6CG282700 Rh6DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 14
Acc65I GGTACC 2 cut(s) 73, 105
AccB1I GGYRCC 2 cut(s) 73, 105
AccBSI CCGCTC 1 cut(s) 766
AciI CCGC 7 cut(s) 192, 223, 406, 409, 675, 766, 781
AcsI RAATTY 1 cut(s) 737
AcuI CTGAAG 1 cut(s) 486
AcyI GRCGYC 1 cut(s) 721
AfaI GTAC 4 cut(s) 75, 107, 492, 504
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 2 cut(s) 430, 585
AjnI CCWGG 2 cut(s) 46, 184
AluBI AGCT 6 cut(s) 64, 89, 248, 326, 545, 611
AluI AGCT 6 cut(s) 64, 89, 248, 326, 545, 611
Alw21I GWGCWC 2 cut(s) 402, 712
Alw26I GTCTC 2 cut(s) 315, 587
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 2 cut(s) 532, 778
ApeKI GCWGC 2 cut(s) 86, 621
ApoI RAATTY 1 cut(s) 737
Asp718I GGTACC 2 cut(s) 73, 105
AspLEI GCGC 2 cut(s) 45, 520
AspS9I GGNCC 1 cut(s) 670
AsuHPI GGTGA 2 cut(s) 270, 653
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 1 cut(s) 670
BanI GGYRCC 2 cut(s) 73, 105
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 2 cut(s) 402, 712
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 98, 633
BciT130I CCWGG 2 cut(s) 48, 186
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 315, 587
BfaI CTAG 4 cut(s) 249, 597, 612, 630
BfmI CTRYAG 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 413
BfuAI ACCTGC 1 cut(s) 14
BisI GCNGC 6 cut(s) 87, 407, 410, 622, 767, 781
BlsI GCNGC 6 cut(s) 88, 408, 411, 623, 768, 782
Bme1390I CCNGG 2 cut(s) 48, 186
Bme18I GGWCC 1 cut(s) 670
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 670
BmiI GGNNCC 5 cut(s) 7, 75, 107, 190, 672
BmrFI CCNGG 2 cut(s) 48, 186
BmsI GCATC 3 cut(s) 129, 361, 427
BmtI GCTAGC 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 267
Bpu10I CCTNAGC 2 cut(s) 90, 546
BpuEI CTTGAG 1 cut(s) 632
BsaBI GATNNNNATC 1 cut(s) 267
BsaHI GRCGYC 1 cut(s) 721
BsaJI CCNNGG 5 cut(s) 46, 110, 149, 184, 616
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 2 cut(s) 42, 660
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 48, 186
BseDI CCNNGG 5 cut(s) 46, 110, 149, 184, 616
BseGI GGATG 1 cut(s) 384
BseJI GATNNNNATC 1 cut(s) 267
BseMII CTCAG 2 cut(s) 81, 560
BseNI ACTGG 2 cut(s) 42, 660
BseXI GCAGC 2 cut(s) 98, 633
BseYI CCCAGC 1 cut(s) 374
BshFI GGCC 2 cut(s) 534, 780
BshNI GGYRCC 2 cut(s) 73, 105
BsiHKAI GWGCWC 2 cut(s) 402, 712
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 521
BslFI GGGAC 1 cut(s) 656
BsmAI GTCTC 2 cut(s) 315, 587
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 2 cut(s) 534, 780
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 402, 712
Bsp1407I TGTACA 1 cut(s) 502
Bsp143I GATC 3 cut(s) 268, 319, 643
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 7 cut(s) 192, 223, 406, 409, 675, 766, 781
BspANI GGCC 2 cut(s) 534, 780
BspCNI CTCAG 2 cut(s) 82, 559
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 5 cut(s) 7, 75, 107, 190, 672
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 14
BspOI GCTAGC 1 cut(s) 615
BspT107I GGYRCC 2 cut(s) 73, 105
BspTI CTTAAG 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 766
BsrFI RCCGGY 1 cut(s) 520
BsrGI TGTACA 1 cut(s) 502
BsrI ACTGG 2 cut(s) 42, 660
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 5 cut(s) 46, 110, 149, 184, 616
BssMI GATC 3 cut(s) 268, 319, 643
BssNI GRCGYC 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 149
Bst2UI CCWGG 2 cut(s) 48, 186
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 1 cut(s) 193
BstACI GRCGYC 1 cut(s) 721
BstAFI CTTAAG 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 502
BstC8I GCNNGC 1 cut(s) 613
BstDEI CTNAG 2 cut(s) 90, 546
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 384
BstHHI GCGC 2 cut(s) 45, 520
BstKTI GATC 3 cut(s) 271, 322, 646
BstMAI GTCTC 2 cut(s) 315, 587
BstMBI GATC 3 cut(s) 268, 319, 643
BstMWI GCNNNNNNNGC 8 cut(s) 20, 61, 126, 397, 406, 415, 621, 630
BstNI CCWGG 2 cut(s) 48, 186
BstSCI CCNGG 2 cut(s) 46, 184
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 98, 633
BstV2I GAAGAC 1 cut(s) 267
BsuRI GGCC 2 cut(s) 534, 780
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 1 cut(s) 384
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 613
CaiI CAGNNNCTG 1 cut(s) 374
CciI TCATGA 1 cut(s) 96
CfoI GCGC 2 cut(s) 45, 520
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 1 cut(s) 670
CseI GACGC 1 cut(s) 710
Csp6I GTAC 4 cut(s) 74, 106, 491, 503
CviAII CATG 2 cut(s) 97, 150
CviQI GTAC 4 cut(s) 74, 106, 491, 503
DdeI CTNAG 2 cut(s) 90, 546
DpnI GATC 3 cut(s) 270, 321, 645
DpnII GATC 3 cut(s) 268, 319, 643
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 149
Eco32I GATATC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 46, 184
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
FaeI CATG 2 cut(s) 100, 153
FaiI YATR 9 cut(s) 11, 98, 151, 212, 434, 480, 692, 731, 759
FaqI GGGAC 1 cut(s) 656
FatI CATG 2 cut(s) 96, 149
FauI CCCGC 1 cut(s) 682
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 6 cut(s) 87, 407, 410, 622, 767, 781
FokI GGATG 1 cut(s) 391
Fsp4HI GCNGC 6 cut(s) 87, 407, 410, 622, 767, 781
FspBI CTAG 4 cut(s) 249, 597, 612, 630
GlaI GCGC 2 cut(s) 44, 519
GluI GCNGC 6 cut(s) 87, 407, 410, 622, 767, 781
GsaI CCCAGC 1 cut(s) 378
HaeIII GGCC 2 cut(s) 534, 780
HapII CCGG 1 cut(s) 521
HgaI GACGC 1 cut(s) 710
HhaI GCGC 2 cut(s) 45, 520
Hin1I GRCGYC 1 cut(s) 721
Hin1II CATG 2 cut(s) 100, 153
Hin6I GCGC 2 cut(s) 43, 518
HinP1I GCGC 2 cut(s) 43, 518
HincII GTYRAC 1 cut(s) 725
HindII GTYRAC 1 cut(s) 725
HinfI GANTC 3 cut(s) 263, 308, 338
HpaII CCGG 1 cut(s) 521
HphI GGTGA 2 cut(s) 270, 653
Hpy166II GTNNAC 3 cut(s) 654, 665, 725
Hpy188I TCNGA 3 cut(s) 262, 268, 315
Hpy188III TCNNGA 2 cut(s) 97, 427
Hpy8I GTNNAC 3 cut(s) 654, 665, 725
HpyAV CCTTC 3 cut(s) 96, 291, 361
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 1 cut(s) 86
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 61, 126, 397, 406, 415, 621, 630
HpyF3I CTNAG 2 cut(s) 90, 546
Hsp92I GRCGYC 1 cut(s) 721
Hsp92II CATG 2 cut(s) 100, 153
HspAI GCGC 2 cut(s) 43, 518
KpnI GGTACC 2 cut(s) 77, 109
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 268, 319, 643
LmnI GCTCC 1 cut(s) 61
Lsp1109I GCAGC 2 cut(s) 98, 633
LweI GCATC 3 cut(s) 129, 361, 427
MaeI CTAG 4 cut(s) 249, 597, 612, 630
MalI GATC 3 cut(s) 270, 321, 645
MbiI CCGCTC 1 cut(s) 766
MboI GATC 3 cut(s) 268, 319, 643
MboII GAAGA 7 cut(s) 130, 199, 210, 267, 479, 507, 573
MhlI GDGCHC 2 cut(s) 402, 712
MluCI AATT 2 cut(s) 78, 737
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 3 cut(s) 85, 105, 626
MseI TTAA 3 cut(s) 162, 243, 414
MspA1I CMGCKG 3 cut(s) 89, 194, 783
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 1 cut(s) 521
MspR9I CCNGG 2 cut(s) 48, 186
MvaI CCWGG 2 cut(s) 48, 186
MwoI GCNNNNNNNGC 8 cut(s) 20, 61, 126, 397, 406, 415, 621, 630
NcoI CCATGG 1 cut(s) 149
NdeII GATC 3 cut(s) 268, 319, 643
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 2 cut(s) 100, 153
NlaIV GGNNCC 5 cut(s) 7, 75, 107, 190, 672
NmeAIII GCCGAG 1 cut(s) 597
PagI TCATGA 1 cut(s) 96
PaqCI CACCTGC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 718
PfeI GAWTC 2 cut(s) 263, 338
PkrI GCNGC 6 cut(s) 88, 408, 411, 623, 768, 782
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 670
Psp5II RGGWCCY 1 cut(s) 670
Psp6I CCWGG 2 cut(s) 46, 184
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 2 cut(s) 46, 184
PspN4I GGNNCC 5 cut(s) 7, 75, 107, 190, 672
PspPI GGNCC 1 cut(s) 670
PspPPI RGGWCCY 1 cut(s) 670
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 4 cut(s) 75, 107, 492, 504
RsaNI GTAC 4 cut(s) 74, 106, 491, 503
SaqAI TTAA 3 cut(s) 162, 243, 414
SatI GCNGC 6 cut(s) 87, 407, 410, 622, 767, 781
Sau3AI GATC 3 cut(s) 268, 319, 643
Sau96I GGNCC 1 cut(s) 670
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 2 cut(s) 48, 186
SduI GDGCHC 2 cut(s) 402, 712
SfaNI GCATC 3 cut(s) 129, 361, 427
SfcI CTRYAG 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 520
SinI GGWCC 1 cut(s) 670
SmlI CTYRAG 2 cut(s) 413, 647
SmoI CTYRAG 2 cut(s) 413, 647
Sse9I AATT 2 cut(s) 78, 737
SsiI CCGC 7 cut(s) 192, 223, 406, 409, 675, 766, 781
SspMI CTAG 4 cut(s) 249, 597, 612, 630
StyD4I CCNGG 2 cut(s) 46, 184
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 2 cut(s) 141, 341
TasI AATT 2 cut(s) 78, 737
TatI WGTACW 1 cut(s) 502
TauI GCSGC 4 cut(s) 409, 412, 769, 783
TfiI GAWTC 2 cut(s) 263, 338
Tru1I TTAA 3 cut(s) 162, 243, 414
Tru9I TTAA 3 cut(s) 162, 243, 414
TseI GCWGC 2 cut(s) 86, 621
TspDTI ATGAA 2 cut(s) 56, 199
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 670
XapI RAATTY 1 cut(s) 737
XspI CTAG 4 cut(s) 249, 597, 612, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.