MD05G1175300.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0017600
Physical Location & Seq
Forward (+)
30305922 .. 30306731
810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1175300.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGGAAGTGTAGTGAGGGAGGAAGGTGTGCTGAAATTGGTGCGTCCAGGGAGGCGTTTGGAGGTTCACAAGAAACCGGTAACAGCTGAAGAGGTGATGAGAAGGTACCCCCGGCACAGCATAACTAGGCCAGACATCTTTGAGTTCCCGTGGATTGTGGTTAAGCCTGAAGCAGTTTTGATTCCCGGGAAAGTGTTTTTTATAGTTCCTAACCGCACGATTTATCAGTTGCTTAAAGCCAGAGAGCACGGCAATCAGCCTCCTCCTTTGATTGAACAGAAGCAATCTGTTAAAGCTCGTGGCCACCATCAAGTACATGAACATAGTTCTCCTCCCAAGGAGTATGCTGATATGACACCGAAGCACCACGAGCAGAGGCGAAGGCTTAAGCTTCAGTTTTTTGCTTGTGCTACTGTAAGCCCTAGCCTGAGTGATGAAGATGCTGACTCTGACAGAAGACTCCAAACAGATATCAAAGTAGAGTTGTCATCCACAAACAAGAAAACCCGCGAAGAATCTAAGCAGAAACCTCCGATACATTCAAAAAATGAAACTAGACGTCGTAGAAAATATGATGATGGCAACAACGACATTACGGTCAAACTTGCTGATCTTAATATTAGGGAGAGCAAGCAAGTCCCCTTGCTGAAATCTTGTGTAAGAAAACCTGAAAGTGCTCGTAAGTCGCTTCGCCTTAAGGTAAAATTTACCTTGCCAAACAAGGAAGAGGAGCGACAAAGCGAGGCTACTGGGTCTCGGGTGCAATTCCAACGCTTCTCGAGTAGATTAGTCCTGCAAGGCATTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

270

Amino Acids

31.31

Weight (kDa)

10.13

Isoelectric Point (pI)

65.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 180 5.4e-11 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19050
malus_domestica MD05G1175300.v1.1 MD10G1164400.v1.1
prunus_persica Prupe.8G203000_v2.0.a1
rosa_laevigata RLG00000012706
rosa_multiflora Rmu_sc0000686.1_g000018
rosa_roxburghii Rroxscaffold_7G00184280
rosa_rugosa Rorug06G0167600
rosa_samantha Rh6AG280400 Rh6BG280700 Rh6CG282700 Rh6DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 596
AatII GACGTC 1 cut(s) 562
Acc65I GGTACC 1 cut(s) 105
AccB1I GGYRCC 1 cut(s) 105
AccII CGCG 1 cut(s) 510
AciI CCGC 2 cut(s) 214, 508
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 1 cut(s) 704
AcuI CTGAAG 3 cut(s) 108, 189, 377
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 2 cut(s) 107, 315
AflII CTTAAG 2 cut(s) 386, 695
AgeI ACCGGT 1 cut(s) 76
AgsI TTSAA 2 cut(s) 275, 543
AjnI CCWGG 1 cut(s) 46
AluBI AGCT 3 cut(s) 86, 296, 391
AluI AGCT 3 cut(s) 86, 296, 391
Alw21I GWGCWC 2 cut(s) 249, 679
Alw26I GTCTC 1 cut(s) 759
Ama87I CYCGRG 3 cut(s) 185, 756, 778
AoxI GGCC 2 cut(s) 128, 301
ApoI RAATTY 1 cut(s) 704
AsiGI ACCGGT 1 cut(s) 76
Asp718I GGTACC 1 cut(s) 105
AsuC2I CCSGG 3 cut(s) 112, 186, 187
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 3 cut(s) 185, 756, 778
BalI TGGCCA 1 cut(s) 303
BanI GGYRCC 1 cut(s) 105
BauI CACGAG 2 cut(s) 297, 368
BbsI GAAGAC 1 cut(s) 463
Bbv12I GWGCWC 2 cut(s) 249, 679
BccI CCATC 2 cut(s) 315, 572
BceAI ACGGC 1 cut(s) 265
BciT130I CCWGG 1 cut(s) 48
BcnI CCSGG 3 cut(s) 112, 186, 187
BcoDI GTCTC 1 cut(s) 759
BfaI CTAG 3 cut(s) 126, 423, 555
BfrI CTTAAG 2 cut(s) 386, 695
Bme1390I CCNGG 4 cut(s) 48, 112, 186, 187
BmeT110I CYCGRG 3 cut(s) 185, 756, 778
BmiI GGNNCC 1 cut(s) 107
BmrFI CCNGG 4 cut(s) 48, 112, 186, 187
BmrI ACTGGG 1 cut(s) 759
BmsI GCATC 1 cut(s) 430
BmuI ACTGGG 1 cut(s) 759
BpiI GAAGAC 1 cut(s) 463
BpuMI CCSGG 3 cut(s) 112, 186, 187
BsaHI GRCGYC 1 cut(s) 559
BsaI GGTCTC 1 cut(s) 759
BsaJI CCNNGG 5 cut(s) 47, 110, 149, 185, 336
BsaWI WCCGGW 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 1 cut(s) 754
BseBI CCWGG 1 cut(s) 48
BseDI CCNNGG 5 cut(s) 47, 110, 149, 185, 336
BseGI GGATG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 1 cut(s) 754
BseRI GAGGAG 3 cut(s) 252, 321, 743
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 2 cut(s) 130, 303
BshNI GGYRCC 1 cut(s) 105
BshTI ACCGGT 1 cut(s) 76
BsiHKAI GWGCWC 2 cut(s) 249, 679
BsiHKCI CYCGRG 3 cut(s) 185, 756, 778
BsiSI CCGG 3 cut(s) 77, 112, 186
BslFI GGGAC 1 cut(s) 623
BsmAI GTCTC 1 cut(s) 759
BsmFI GGGAC 1 cut(s) 623
BsnI GGCC 2 cut(s) 130, 303
Bso31I GGTCTC 1 cut(s) 759
BsoBI CYCGRG 3 cut(s) 185, 756, 778
Bsp1286I GDGCHC 2 cut(s) 249, 679
Bsp143I GATC 1 cut(s) 610
BspACI CCGC 2 cut(s) 214, 508
BspANI GGCC 2 cut(s) 130, 303
BspCNI CTCAG 1 cut(s) 420
BspFNI CGCG 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 105
BspTI CTTAAG 2 cut(s) 386, 695
BspTNI GGTCTC 1 cut(s) 759
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 1 cut(s) 754
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 5 cut(s) 47, 110, 149, 185, 336
BssMI GATC 1 cut(s) 610
BssNI GRCGYC 1 cut(s) 559
BssSI CACGAG 2 cut(s) 297, 368
BssT1I CCWWGG 1 cut(s) 336
Bst2BI CACGAG 2 cut(s) 297, 368
Bst2UI CCWGG 1 cut(s) 48
Bst4CI ACNGT 2 cut(s) 415, 598
Bst6I CTCTTC 2 cut(s) 84, 720
BstACI GRCGYC 1 cut(s) 559
BstAFI CTTAAG 2 cut(s) 386, 695
BstC8I GCNNGC 1 cut(s) 632
BstDEI CTNAG 2 cut(s) 428, 519
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 488
BstFNI CGCG 1 cut(s) 510
BstKTI GATC 1 cut(s) 613
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 1 cut(s) 610
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 1 cut(s) 48
BstSCI CCNGG 4 cut(s) 46, 110, 184, 185
BstUI CGCG 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 463
BsuRI GGCC 2 cut(s) 130, 303
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 1 cut(s) 488
Cac8I GCNNGC 1 cut(s) 632
Cfr10I RCCGGY 1 cut(s) 76
Cfr9I CCCGGG 1 cut(s) 185
CseI GACGC 1 cut(s) 32
Csp6I GTAC 2 cut(s) 106, 314
CspAI ACCGGT 1 cut(s) 76
CviAII CATG 1 cut(s) 317
CviQI GTAC 2 cut(s) 106, 314
DdeI CTNAG 2 cut(s) 428, 519
DpnI GATC 1 cut(s) 612
DpnII GATC 1 cut(s) 610
DrdI GACNNNNNNGTC 1 cut(s) 596
DseDI GACNNNNNNGTC 1 cut(s) 596
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 84, 720
EarI CTCTTC 2 cut(s) 84, 720
Eco130I CCWWGG 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 759
Eco32I GATATC 1 cut(s) 472
Eco57I CTGAAG 3 cut(s) 108, 189, 377
Eco88I CYCGRG 3 cut(s) 185, 756, 778
EcoRII CCWGG 1 cut(s) 46
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 1 cut(s) 320
FaiI YATR 7 cut(s) 122, 203, 318, 324, 345, 353, 573
FaqI GGGAC 1 cut(s) 623
FatI CATG 1 cut(s) 316
FauI CCCGC 1 cut(s) 515
FokI GGATG 1 cut(s) 475
FspBI CTAG 3 cut(s) 126, 423, 555
HaeIII GGCC 2 cut(s) 130, 303
HapII CCGG 3 cut(s) 77, 112, 186
HgaI GACGC 1 cut(s) 32
Hin1I GRCGYC 1 cut(s) 559
Hin1II CATG 1 cut(s) 320
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 4 cut(s) 181, 446, 459, 515
HpaII CCGG 3 cut(s) 77, 112, 186
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 451, 534
Hpy188III TCNNGA 1 cut(s) 778
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 564
HpyAV CCTTC 3 cut(s) 17, 96, 375
HpyCH4III ACNGT 2 cut(s) 415, 598
HpyCH4IV ACGT 1 cut(s) 559
HpyCH4V TGCA 2 cut(s) 763, 796
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpyF3I CTNAG 2 cut(s) 428, 519
HpySE526I ACGT 1 cut(s) 559
Hsp92I GRCGYC 1 cut(s) 559
Hsp92II CATG 1 cut(s) 320
KpnI GGTACC 1 cut(s) 109
Kzo9I GATC 1 cut(s) 610
LmnI GCTCC 1 cut(s) 730
LweI GCATC 1 cut(s) 430
MaeI CTAG 3 cut(s) 126, 423, 555
MaeII ACGT 1 cut(s) 559
MaeIII GTNAC 1 cut(s) 79
MalI GATC 1 cut(s) 612
MboI GATC 1 cut(s) 610
MboII GAAGA 5 cut(s) 101, 449, 468, 524, 737
MhlI GDGCHC 2 cut(s) 249, 679
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 3 cut(s) 35, 704, 764
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 2 cut(s) 440, 453
MmeI TCCRAC 1 cut(s) 793
Mox20I TGGCCA 1 cut(s) 303
MscI TGGCCA 1 cut(s) 303
MseI TTAA 7 cut(s) 162, 234, 291, 387, 615, 696, 808
Msp20I TGGCCA 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 86
MspCI CTTAAG 2 cut(s) 386, 695
MspI CCGG 3 cut(s) 77, 112, 186
MspR9I CCNGG 4 cut(s) 48, 112, 186, 187
MvaI CCWGG 1 cut(s) 48
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 1 cut(s) 370
NciI CCSGG 3 cut(s) 112, 186, 187
NdeII GATC 1 cut(s) 610
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 1 cut(s) 107
PaeR7I CTCGAG 1 cut(s) 778
PfeI GAWTC 2 cut(s) 181, 515
PinAI ACCGGT 1 cut(s) 76
PleI GAGTC 2 cut(s) 440, 453
PpsI GAGTC 2 cut(s) 440, 453
Psp6I CCWGG 1 cut(s) 46
PspGI CCWGG 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 2 cut(s) 107, 315
RsaNI GTAC 2 cut(s) 106, 314
SaqAI TTAA 7 cut(s) 162, 234, 291, 387, 615, 696, 808
Sau3AI GATC 1 cut(s) 610
SchI GAGTC 2 cut(s) 440, 453
ScrFI CCNGG 4 cut(s) 48, 112, 186, 187
SduI GDGCHC 2 cut(s) 249, 679
SfaNI GCATC 1 cut(s) 430
Sfr274I CTCGAG 1 cut(s) 778
SlaI CTCGAG 1 cut(s) 778
SmaI CCCGGG 1 cut(s) 187
SmlI CTYRAG 3 cut(s) 386, 695, 778
SmoI CTYRAG 3 cut(s) 386, 695, 778
Sse9I AATT 3 cut(s) 35, 704, 764
SsiI CCGC 2 cut(s) 214, 508
SspI AATATT 1 cut(s) 619
SspMI CTAG 3 cut(s) 126, 423, 555
StyD4I CCNGG 4 cut(s) 46, 110, 184, 185
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 2 cut(s) 415, 598
TaiI ACGT 1 cut(s) 562
TaqI TCGA 1 cut(s) 779
TasI AATT 3 cut(s) 35, 704, 764
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 2 cut(s) 181, 515
Tru1I TTAA 7 cut(s) 162, 234, 291, 387, 615, 696, 808
Tru9I TTAA 7 cut(s) 162, 234, 291, 387, 615, 696, 808
TspDTI ATGAA 3 cut(s) 333, 450, 564
TspMI CCCGGG 1 cut(s) 185
Vha464I CTTAAG 2 cut(s) 386, 695
XapI RAATTY 1 cut(s) 704
XhoI CTCGAG 1 cut(s) 778
XmaI CCCGGG 1 cut(s) 185
XspI CTAG 3 cut(s) 126, 423, 555
ZraI GACGTC 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.