Rh6CG282700

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0017600
Physical Location & Seq
Forward (+)
46006287 .. 46007117
831 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG282700.1

Sequence Viewer

Length: 831 bp
ATGGGAACCATAGCAAGGGAAGCAGGTGTGCTGAAACTGGTGCGCCCTGGTAGGCGAGTGGAGCTTCATCGGGTACCGATTACTGCAGCTGAGGTCATGAGAAGGTACCCGAGGCACAGCATCACTCGCCCTGATATCTTCGAGTTCCCATGGATTGTGGTTAAGCCAGAAGCAGTTTTGACCCCTGGAACCGCTGGAAGAGTGTTCTTCATAGTTCCTAATCGCACTATCTATCAGTTGCTTAAAGCTAGAGAGAACTCTGATTCTGATCAGTCTTCACCTTCTGCTTCGCAACAGAAACAACAAGAGTCTCTGAAAGATCAAGCTCAACAACAAGTATTGAAAAAGACCTTCCCACCCAACAGGACAGATGCTGGGATGACACCAAAGCACCGAGAGCACAGGCGACGGCTTAAGCATCAGTTCTTGAGCATATCTTGTGCTACTACTGTGTGTCCTACTGAAGAACAAGACTATGACAAAGAAGTACGAAGAAATGTACAAAGGGTGTCTGCGCCGGTGTTGCCAGGCCACAAGACAGCTCAGGGAGAACCTAAACGAAAATCATCATCTCTATCTTCAAAAAATGAGACTAGAAAAGGTAAAGGTAAAGCTGGCCTCAGTAGCATTACTGGCAAACTTGGTGATCTCAAGTTTACAACCAGTAAACAGGTCCCCGCACTGAAATCTTGTATAAGAAAACCAGATAGTGCTCGTAAGTTGCGTCAACTTATAGTGAAATTTGACTTGCCAGACGACTATGTGGAGCGGCAAACCAAGGCCGCTGATTGTCAGATGCATCGGATGCAATATCCAGACTATAAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

276

Amino Acids

31.42

Weight (kDa)

10.18

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 7 - 169 4.1e-12 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017600)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g19050
malus_domestica MD05G1175300.v1.1 MD10G1164400.v1.1
prunus_persica Prupe.8G203000_v2.0.a1
rosa_laevigata RLG00000012706
rosa_multiflora Rmu_sc0000686.1_g000018
rosa_roxburghii Rroxscaffold_7G00184280
rosa_rugosa Rorug06G0167600
rosa_samantha Rh6AG280400 Rh6BG280700 Rh6CG282700 Rh6DG275800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 14
Acc65I GGTACC 2 cut(s) 73, 105
AccB1I GGYRCC 2 cut(s) 73, 105
AccBSI CCGCTC 1 cut(s) 769
AciI CCGC 4 cut(s) 192, 678, 769, 783
AcsI RAATTY 1 cut(s) 740
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 4 cut(s) 75, 107, 489, 501
AfiI CCNNNNNNNGG 1 cut(s) 15
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 2 cut(s) 343, 582
AjnI CCWGG 3 cut(s) 46, 184, 526
AluBI AGCT 6 cut(s) 64, 89, 248, 326, 542, 614
AluI AGCT 6 cut(s) 64, 89, 248, 326, 542, 614
Alw21I GWGCWC 2 cut(s) 402, 715
Alw26I GTCTC 2 cut(s) 315, 584
AlwNI CAGNNNCTG 1 cut(s) 374
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 3 cut(s) 529, 616, 780
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 740
Asp718I GGTACC 2 cut(s) 73, 105
AspLEI GCGC 2 cut(s) 45, 517
AspS9I GGNCC 1 cut(s) 673
AsuHPI GGTGA 2 cut(s) 270, 656
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 1 cut(s) 673
BanI GGYRCC 2 cut(s) 73, 105
BbsI GAAGAC 1 cut(s) 267
Bbv12I GWGCWC 2 cut(s) 402, 715
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 98
BceAI ACGGC 1 cut(s) 425
BciT130I CCWGG 3 cut(s) 48, 186, 528
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 315, 584
BfaI CTAG 2 cut(s) 249, 594
BfmI CTRYAG 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 413
BfuAI ACCTGC 1 cut(s) 14
BisI GCNGC 3 cut(s) 87, 770, 783
BlsI GCNGC 3 cut(s) 88, 771, 784
Bme1390I CCNGG 3 cut(s) 48, 186, 528
Bme18I GGWCC 1 cut(s) 673
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 673
BmiI GGNNCC 5 cut(s) 7, 75, 107, 190, 675
BmrFI CCNGG 3 cut(s) 48, 186, 528
BmsI GCATC 6 cut(s) 129, 361, 427, 786, 795, 808
BpiI GAAGAC 1 cut(s) 267
Bpu10I CCTNAGC 2 cut(s) 90, 543
BpuEI CTTGAG 2 cut(s) 448, 635
BsaBI GATNNNNATC 1 cut(s) 267
BsaJI CCNNGG 5 cut(s) 46, 110, 149, 184, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 3 cut(s) 42, 637, 663
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 3 cut(s) 48, 186, 528
BseDI CCNNGG 5 cut(s) 46, 110, 149, 184, 777
BseGI GGATG 2 cut(s) 384, 810
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 81, 557, 634
BseNI ACTGG 3 cut(s) 42, 637, 663
BseXI GCAGC 1 cut(s) 98
BseYI CCCAGC 1 cut(s) 374
BshFI GGCC 3 cut(s) 531, 618, 782
BshNI GGYRCC 2 cut(s) 73, 105
BsiHKAI GWGCWC 2 cut(s) 402, 715
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 2 cut(s) 315, 584
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 3 cut(s) 531, 618, 782
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 402, 715
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 3 cut(s) 268, 319, 646
Bsp19I CCATGG 1 cut(s) 149
BspACI CCGC 4 cut(s) 192, 678, 769, 783
BspANI GGCC 3 cut(s) 531, 618, 782
BspCNI CTCAG 3 cut(s) 82, 556, 633
BspHI TCATGA 1 cut(s) 96
BspLI GGNNCC 5 cut(s) 7, 75, 107, 190, 675
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 14
BspT107I GGYRCC 2 cut(s) 73, 105
BspTI CTTAAG 1 cut(s) 413
BsrBI CCGCTC 1 cut(s) 769
BsrFI RCCGGY 1 cut(s) 517
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 3 cut(s) 42, 637, 663
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 5 cut(s) 46, 110, 149, 184, 777
BssMI GATC 3 cut(s) 268, 319, 646
BssT1I CCWWGG 2 cut(s) 149, 777
Bst2UI CCWGG 3 cut(s) 48, 186, 528
Bst4CI ACNGT 1 cut(s) 451
Bst6I CTCTTC 1 cut(s) 193
BstAFI CTTAAG 1 cut(s) 413
BstAPI GCANNNNNTGC 1 cut(s) 805
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 1 cut(s) 616
BstDEI CTNAG 3 cut(s) 90, 543, 620
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 2 cut(s) 384, 810
BstHHI GCGC 2 cut(s) 45, 517
BstKTI GATC 3 cut(s) 271, 322, 649
BstMAI GTCTC 2 cut(s) 315, 584
BstMBI GATC 3 cut(s) 268, 319, 646
BstMWI GCNNNNNNNGC 8 cut(s) 20, 61, 126, 397, 523, 624, 633, 805
BstNI CCWGG 3 cut(s) 48, 186, 528
BstSCI CCNGG 3 cut(s) 46, 184, 526
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 98
BstV2I GAAGAC 1 cut(s) 267
BsuRI GGCC 3 cut(s) 531, 618, 782
BtgI CCRYGG 1 cut(s) 149
BtsCI GGATG 2 cut(s) 384, 810
BtsIMutI CAGTG 1 cut(s) 680
BveI ACCTGC 1 cut(s) 14
Cac8I GCNNGC 1 cut(s) 616
CaiI CAGNNNCTG 1 cut(s) 374
CciI TCATGA 1 cut(s) 96
CfoI GCGC 2 cut(s) 45, 517
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 1 cut(s) 673
CseI GACGC 1 cut(s) 713
Csp6I GTAC 4 cut(s) 74, 106, 488, 500
CviAII CATG 2 cut(s) 97, 150
CviQI GTAC 4 cut(s) 74, 106, 488, 500
DdeI CTNAG 3 cut(s) 90, 543, 620
DpnI GATC 3 cut(s) 270, 321, 648
DpnII GATC 3 cut(s) 268, 319, 646
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 149, 777
Eco32I GATATC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 673
EcoRII CCWGG 3 cut(s) 46, 184, 526
EcoRV GATATC 1 cut(s) 136
EcoT14I CCWWGG 2 cut(s) 149, 777
EcoT22I ATGCAT 1 cut(s) 801
ErhI CCWWGG 2 cut(s) 149, 777
FaeI CATG 2 cut(s) 100, 153
FaqI GGGAC 1 cut(s) 659
FatI CATG 2 cut(s) 96, 149
FauI CCCGC 1 cut(s) 685
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 87, 770, 783
FokI GGATG 2 cut(s) 391, 817
Fsp4HI GCNGC 3 cut(s) 87, 770, 783
FspBI CTAG 2 cut(s) 249, 594
GlaI GCGC 2 cut(s) 44, 516
GluI GCNGC 3 cut(s) 87, 770, 783
GsaI CCCAGC 1 cut(s) 378
HaeIII GGCC 3 cut(s) 531, 618, 782
HapII CCGG 1 cut(s) 518
HgaI GACGC 1 cut(s) 713
HhaI GCGC 2 cut(s) 45, 517
Hin1II CATG 2 cut(s) 100, 153
Hin6I GCGC 2 cut(s) 43, 515
HinP1I GCGC 2 cut(s) 43, 515
HincII GTYRAC 1 cut(s) 728
HindII GTYRAC 1 cut(s) 728
HinfI GANTC 2 cut(s) 263, 308
HpaII CCGG 1 cut(s) 518
HphI GGTGA 2 cut(s) 270, 656
Hpy166II GTNNAC 3 cut(s) 657, 668, 728
Hpy188I TCNGA 5 cut(s) 262, 268, 315, 795, 804
Hpy188III TCNNGA 3 cut(s) 97, 427, 815
Hpy8I GTNNAC 3 cut(s) 657, 668, 728
Hpy99I CGWCG 1 cut(s) 411
HpyAV CCTTC 3 cut(s) 96, 291, 361
HpyCH4III ACNGT 1 cut(s) 451
HpyCH4V TGCA 3 cut(s) 86, 799, 808
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 61, 126, 397, 523, 624, 633, 805
HpyF3I CTNAG 3 cut(s) 90, 543, 620
Hsp92II CATG 2 cut(s) 100, 153
HspAI GCGC 2 cut(s) 43, 515
KpnI GGTACC 2 cut(s) 77, 109
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 268, 319, 646
LmnI GCTCC 2 cut(s) 61, 766
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 6 cut(s) 129, 361, 427, 786, 795, 808
MaeI CTAG 2 cut(s) 249, 594
MalI GATC 3 cut(s) 270, 321, 648
MbiI CCGCTC 1 cut(s) 769
MboI GATC 3 cut(s) 268, 319, 646
MboII GAAGA 7 cut(s) 130, 199, 210, 267, 476, 504, 570
MhlI GDGCHC 2 cut(s) 402, 715
MluCI AATT 1 cut(s) 740
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 3 cut(s) 85, 105, 629
Mph1103I ATGCAT 1 cut(s) 801
MseI TTAA 3 cut(s) 162, 243, 414
MspA1I CMGCKG 3 cut(s) 89, 194, 785
MspCI CTTAAG 1 cut(s) 413
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 3 cut(s) 48, 186, 528
MvaI CCWGG 3 cut(s) 48, 186, 528
MwoI GCNNNNNNNGC 8 cut(s) 20, 61, 126, 397, 523, 624, 633, 805
NcoI CCATGG 1 cut(s) 149
NdeII GATC 3 cut(s) 268, 319, 646
NlaIII CATG 2 cut(s) 100, 153
NlaIV GGNNCC 5 cut(s) 7, 75, 107, 190, 675
NsiI ATGCAT 1 cut(s) 801
PagI TCATGA 1 cut(s) 96
PaqCI CACCTGC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 721
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 3 cut(s) 88, 771, 784
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PpuMI RGGWCCY 1 cut(s) 673
Psp5II RGGWCCY 1 cut(s) 673
Psp6I CCWGG 3 cut(s) 46, 184, 526
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 3 cut(s) 46, 184, 526
PspN4I GGNNCC 5 cut(s) 7, 75, 107, 190, 675
PspPI GGNCC 1 cut(s) 673
PspPPI RGGWCCY 1 cut(s) 673
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 4 cut(s) 75, 107, 489, 501
RsaNI GTAC 4 cut(s) 74, 106, 488, 500
SaqAI TTAA 3 cut(s) 162, 243, 414
SatI GCNGC 3 cut(s) 87, 770, 783
Sau3AI GATC 3 cut(s) 268, 319, 646
Sau96I GGNCC 1 cut(s) 673
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 3 cut(s) 48, 186, 528
SduI GDGCHC 2 cut(s) 402, 715
SfaNI GCATC 6 cut(s) 129, 361, 427, 786, 795, 808
SfcI CTRYAG 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 517
SinI GGWCC 1 cut(s) 673
SmlI CTYRAG 3 cut(s) 413, 427, 650
SmoI CTYRAG 3 cut(s) 413, 427, 650
Sse9I AATT 1 cut(s) 740
SsiI CCGC 4 cut(s) 192, 678, 769, 783
SspI AATATT 1 cut(s) 827
SspMI CTAG 2 cut(s) 249, 594
StyD4I CCNGG 3 cut(s) 46, 184, 526
StyI CCWWGG 2 cut(s) 149, 777
TaaI ACNGT 1 cut(s) 451
TaqI TCGA 1 cut(s) 141
TasI AATT 1 cut(s) 740
TatI WGTACW 1 cut(s) 499
TauI GCSGC 2 cut(s) 772, 785
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 162, 243, 414
Tru9I TTAA 3 cut(s) 162, 243, 414
TscAI CASTG 1 cut(s) 687
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 2 cut(s) 56, 199
TspRI CASTG 1 cut(s) 687
Vha464I CTTAAG 1 cut(s) 413
VpaK11BI GGWCC 1 cut(s) 673
XapI RAATTY 1 cut(s) 740
XspI CTAG 2 cut(s) 249, 594
Zsp2I ATGCAT 1 cut(s) 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.