FvH4_2g19490

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016259
Physical Location & Seq
Forward (+)
16529670 .. 16530803
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19490.t1

Sequence Viewer

Length: 1134 bp
ATGCTCTTGCTTCCTCCTCCTAGGACCCGAACCCTAGAAACTAGAAGAACATTGTGCAGCATTCTTCAAGGAACAACCTACAACAATATTCAGATACAAGTTCCTCTTCCCAAAACCAACGAGTGTACGATAATCAGTACATACTGTCGTGGTTCTGGCCACGGTTGGTTGGCCAAAGTAAGAGTATATGATTATTATGTTGAAAACTCTGATCCTGAATGGAGTATTGAACTCGTGAACCCTTTTCGGCAAGTAACAAGTGTTAGTGTTCATGCCATCCCTCCTATTAAACTCCGACTTGATGATTCTGATAGATCGAATGTTCCGAAGGTTATATTGTCCTCTGATCCAGCTTTGAATCCGGACAAGTTTTGGGTTTTAGCATTCCTCACTCGAGGTTCCCAGCTTGCTTTCCATAAAAGAGGACAAGATGGTTGGACTCTCATACCAACAGGTGGACTATGGTTTAGTGATGCTATATTTTACGAAGGAAGTTTCTGTGCAATTAGTGATCAGTATAACTCATTGCATGGTAGTCTCCTAGTGTTCGATGTTGAAAAAGTCAGGTGGCATATACTTAGAATTGGTGGGTTTGAAATACTATGTTCAAATCGCACCTATTTGGTAGAATCAACAAAGGGATATCTATTGTGTGTCAAGAGAATTGTGAAATGTATACCAGGTGATTATGGTCTGAAGAACGTGTCGACGGAGAGCTTCAAGGTCTACAAGTTGGAGTTGGACAACCTGTCGTCGTCAACCACAGTGACTCCGGTCGAGGTCATAAACATGGAGGATGAGACTTTGTTTGTAGGTGACACTCCATCGGTATCTGTCGTGGCTTCAGACTTCTGTGGATATCAACCGAATTCGATTTACTTTACCGATGATTCGATGAACATTCCTGTTATTTTGGATAGTTTGCATATAAAAGAGCCGCAAGGGATTGAATATGACATGGGCGTCTTTAGTTTAGCAGATCAAAGTATCAAGTCATTTAATGGAAGGCACTTCAGACCTTCAGTATGGATTACGACACCTCCGCCGTATGATCCATCACCAGGATCAAACACGTTAAAGTTGACAAGAGCAAGCCAGGAAGACAAGAAGGGACACAACCACATTCCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

378

Amino Acids

42.42

Weight (kDa)

6.01

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 33 - 323 1e-39 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 962
AccB7I CCANNNNNTGG 1 cut(s) 455
AccI GTMKAC 3 cut(s) 676, 707, 726
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 2 cut(s) 938, 1043
AclWI GGATC 4 cut(s) 206, 341, 1046, 1072
AcoI YGGCCR 2 cut(s) 157, 171
AcsI RAATTY 1 cut(s) 868
AcuI CTGAAG 4 cut(s) 716, 828, 997, 1005
AcyI GRCGYC 1 cut(s) 963
AfaI GTAC 2 cut(s) 127, 139
AfiI CCNNNNNNNGG 2 cut(s) 455, 1061
AflIII ACRYGT 2 cut(s) 702, 1071
AgsI TTSAA 9 cut(s) 68, 203, 230, 358, 557, 596, 609, 721, 950
AhdI GACNNNNNGTC 1 cut(s) 748
AjnI CCWGG 3 cut(s) 679, 1060, 1095
AluBI AGCT 3 cut(s) 353, 406, 717
AluI AGCT 3 cut(s) 353, 406, 717
Alw26I GTCTC 2 cut(s) 542, 794
AlwI GGATC 4 cut(s) 206, 341, 1046, 1072
Ama87I CYCGRG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 2 cut(s) 157, 171
ApeKI GCWGC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 868
AspA2I CCTAGG 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 24
AsuHPI GGTGA 3 cut(s) 695, 827, 1050
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 1 cut(s) 24
AvrII CCTAGG 1 cut(s) 20
BalI TGGCCA 2 cut(s) 159, 173
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 1107
BbvI GCAGC 1 cut(s) 69
BccI CCATC 4 cut(s) 284, 425, 832, 1063
BceAI ACGGC 1 cut(s) 1030
BciT130I CCWGG 3 cut(s) 681, 1062, 1097
BclI TGATCA 1 cut(s) 511
BcoDI GTCTC 2 cut(s) 542, 794
BfaI CTAG 4 cut(s) 21, 35, 42, 542
BisI GCNGC 2 cut(s) 58, 938
BlnI CCTAGG 1 cut(s) 20
BlsI GCNGC 2 cut(s) 59, 939
Bme1390I CCNGG 3 cut(s) 681, 1062, 1097
Bme18I GGWCC 1 cut(s) 24
BmeRI GACNNNNNGTC 1 cut(s) 748
BmeT110I CYCGRG 1 cut(s) 393
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 2 cut(s) 26, 400
BmrFI CCNGG 3 cut(s) 681, 1062, 1097
BmsI GCATC 1 cut(s) 463
BoxI GACNNNNGTC 1 cut(s) 773
BpiI GAAGAC 1 cut(s) 1107
BsaHI GRCGYC 1 cut(s) 963
BsaJI CCNNGG 2 cut(s) 20, 160
BsaWI WCCGGW 2 cut(s) 361, 772
BsaXI ACNNNNNCTCC 4 cut(s) 754, 784, 1024, 1054
Bsc4I CCNNNNNNNGG 2 cut(s) 455, 1061
Bse3DI GCAATG 1 cut(s) 524
BseAI TCCGGA 1 cut(s) 361
BseBI CCWGG 3 cut(s) 681, 1062, 1097
BseDI CCNNGG 2 cut(s) 20, 160
BseGI GGATG 2 cut(s) 276, 802
BseLI CCNNNNNNNGG 2 cut(s) 455, 1061
BseMI GCAATG 1 cut(s) 524
BseRI GAGGAG 1 cut(s) 6
BseXI GCAGC 1 cut(s) 69
BseYI CCCAGC 1 cut(s) 402
BsgI GTGCAG 1 cut(s) 76
Bsh1285I CGRYCG 1 cut(s) 777
BshFI GGCC 2 cut(s) 159, 173
BsiEI CGRYCG 1 cut(s) 777
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 2 cut(s) 362, 773
BslFI GGGAC 1 cut(s) 1125
BslI CCNNNNNNNGG 2 cut(s) 455, 1061
BsmAI GTCTC 2 cut(s) 542, 794
BsmFI GGGAC 1 cut(s) 1125
BsmI GAATGC 2 cut(s) 60, 383
BsnI GGCC 2 cut(s) 159, 173
BsoBI CYCGRG 1 cut(s) 393
Bsp13I TCCGGA 1 cut(s) 361
Bsp143I GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
BspACI CCGC 2 cut(s) 938, 1043
BspANI GGCC 2 cut(s) 159, 173
BspEI TCCGGA 1 cut(s) 361
BspLI GGNNCC 2 cut(s) 26, 400
BspPI GGATC 4 cut(s) 206, 341, 1046, 1072
BsrDI GCAATG 1 cut(s) 524
BssECI CCNNGG 2 cut(s) 20, 160
BssMI GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
BssNAI GTATAC 1 cut(s) 677
BssNI GRCGYC 1 cut(s) 963
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 20
Bst1107I GTATAC 1 cut(s) 677
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 3 cut(s) 681, 1062, 1097
Bst4CI ACNGT 3 cut(s) 146, 164, 766
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 963
BstC8I GCNNGC 2 cut(s) 408, 1093
BstDEI CTNAG 1 cut(s) 578
BstDSI CCRYGG 1 cut(s) 160
BstF5I GGATG 2 cut(s) 276, 802
BstKTI GATC 7 cut(s) 214, 317, 349, 514, 982, 1054, 1067
BstMAI GTCTC 2 cut(s) 542, 794
BstMBI GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
BstMCI CGRYCG 1 cut(s) 777
BstNI CCWGG 3 cut(s) 681, 1062, 1097
BstPAI GACNNNNGTC 1 cut(s) 773
BstSCI CCNGG 3 cut(s) 679, 1060, 1095
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 1 cut(s) 1107
BstZ17I GTATAC 1 cut(s) 677
BsuRI GGCC 2 cut(s) 159, 173
BtgI CCRYGG 1 cut(s) 160
BtsCI GGATG 2 cut(s) 276, 802
BtsIMutI CAGTG 1 cut(s) 771
Cac8I GCNNGC 2 cut(s) 408, 1093
Cfr13I GGNCC 1 cut(s) 24
CseI GACGC 1 cut(s) 952
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 2 cut(s) 126, 138
CviAII CATG 4 cut(s) 272, 530, 790, 958
CviJI RGCY 8 cut(s) 159, 173, 353, 406, 717, 842, 937, 1095
CviKI_1 RGCY 8 cut(s) 159, 173, 353, 406, 717, 842, 937, 1095
CviQI GTAC 2 cut(s) 126, 138
DdeI CTNAG 1 cut(s) 578
DpnI GATC 7 cut(s) 213, 316, 348, 513, 981, 1053, 1066
DpnII GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
DrdI GACNNNNNNGTC 1 cut(s) 962
DriI GACNNNNNGTC 1 cut(s) 748
DseDI GACNNNNNNGTC 1 cut(s) 962
EaeI YGGCCR 2 cut(s) 157, 171
Eam1104I CTCTTC 1 cut(s) 111
Eam1105I GACNNNNNGTC 1 cut(s) 748
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 1032
Eco130I CCWWGG 1 cut(s) 20
Eco32I GATATC 2 cut(s) 644, 860
Eco47I GGWCC 1 cut(s) 24
Eco57I CTGAAG 4 cut(s) 716, 828, 997, 1005
Eco88I CYCGRG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 868
EcoRII CCWGG 3 cut(s) 679, 1060, 1095
EcoRV GATATC 2 cut(s) 644, 860
EcoT14I CCWWGG 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 4 cut(s) 275, 533, 793, 961
FalI AAGNNNNNCTT 2 cut(s) 90, 122
FaqI GGGAC 1 cut(s) 1125
FatI CATG 4 cut(s) 271, 529, 789, 957
FbaI TGATCA 1 cut(s) 511
FblI GTMKAC 3 cut(s) 676, 707, 726
Fnu4HI GCNGC 2 cut(s) 58, 938
FokI GGATG 2 cut(s) 263, 809
Fsp4HI GCNGC 2 cut(s) 58, 938
FspBI CTAG 4 cut(s) 21, 35, 42, 542
GluI GCNGC 2 cut(s) 58, 938
GsaI CCCAGC 1 cut(s) 406
HaeIII GGCC 2 cut(s) 159, 173
HapII CCGG 2 cut(s) 362, 773
HgaI GACGC 1 cut(s) 952
Hin1I GRCGYC 1 cut(s) 963
Hin1II CATG 4 cut(s) 275, 533, 793, 961
HincII GTYRAC 3 cut(s) 708, 759, 1083
HindII GTYRAC 3 cut(s) 708, 759, 1083
HinfI GANTC 6 cut(s) 305, 358, 439, 629, 769, 890
HpaII CCGG 2 cut(s) 362, 773
HphI GGTGA 3 cut(s) 695, 827, 1050
Hpy166II GTNNAC 8 cut(s) 126, 238, 458, 677, 708, 727, 759, 1083
Hpy188I TCNGA 9 cut(s) 93, 211, 296, 310, 327, 346, 696, 847, 1016
Hpy188III TCNNGA 5 cut(s) 215, 235, 362, 658, 1127
Hpy8I GTNNAC 8 cut(s) 126, 238, 458, 677, 708, 727, 759, 1083
Hpy99I CGWCG 2 cut(s) 712, 757
HpyAV CCTTC 5 cut(s) 322, 482, 999, 1029, 1102
HpyCH4III ACNGT 3 cut(s) 146, 164, 766
HpyCH4IV ACGT 2 cut(s) 702, 1073
HpyCH4V TGCA 4 cut(s) 57, 503, 529, 925
HpyF3I CTNAG 1 cut(s) 578
HpySE526I ACGT 2 cut(s) 702, 1073
Hsp92I GRCGYC 1 cut(s) 963
Hsp92II CATG 4 cut(s) 275, 533, 793, 961
Kpn2I TCCGGA 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 1 cut(s) 463
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 4 cut(s) 21, 35, 42, 542
MaeII ACGT 2 cut(s) 702, 1073
MaeIII GTNAC 3 cut(s) 253, 766, 815
MalI GATC 7 cut(s) 213, 316, 348, 513, 981, 1053, 1066
MboI GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
MboII GAAGA 5 cut(s) 56, 57, 98, 709, 1112
MlsI TGGCCA 2 cut(s) 159, 173
MluCI AATT 4 cut(s) 504, 582, 663, 868
MluNI TGGCCA 2 cut(s) 159, 173
MlyI GAGTC 2 cut(s) 433, 763
MmeI TCCRAC 4 cut(s) 319, 416, 714, 720
Mox20I TGGCCA 2 cut(s) 159, 173
MroI TCCGGA 1 cut(s) 361
MscI TGGCCA 2 cut(s) 159, 173
MseI TTAA 4 cut(s) 288, 999, 1076, 1132
MslI CAYNNNNRTG 1 cut(s) 788
Msp20I TGGCCA 2 cut(s) 159, 173
MspI CCGG 2 cut(s) 362, 773
MspR9I CCNGG 3 cut(s) 681, 1062, 1097
Mva1269I GAATGC 2 cut(s) 60, 383
MvaI CCWGG 3 cut(s) 681, 1062, 1097
NdeII GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
NlaIII CATG 4 cut(s) 275, 533, 793, 961
NlaIV GGNNCC 2 cut(s) 26, 400
NmuCI GTSAC 2 cut(s) 766, 815
PaeR7I CTCGAG 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 323
PctI GAATGC 2 cut(s) 60, 383
PfeI GAWTC 4 cut(s) 305, 358, 629, 890
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 2 cut(s) 59, 939
PleI GAGTC 2 cut(s) 433, 763
PpsI GAGTC 2 cut(s) 433, 763
PpuMI RGGWCCY 1 cut(s) 24
PshAI GACNNNNGTC 1 cut(s) 773
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 3 cut(s) 679, 1060, 1095
PspFI CCCAGC 1 cut(s) 402
PspGI CCWGG 3 cut(s) 679, 1060, 1095
PspN4I GGNNCC 2 cut(s) 26, 400
PspPI GGNCC 1 cut(s) 24
PspPPI RGGWCCY 1 cut(s) 24
PspXI VCTCGAGB 1 cut(s) 393
RsaI GTAC 2 cut(s) 127, 139
RsaNI GTAC 2 cut(s) 126, 138
RseI CAYNNNNRTG 1 cut(s) 788
SalI GTCGAC 1 cut(s) 706
SaqAI TTAA 4 cut(s) 288, 999, 1076, 1132
SatI GCNGC 2 cut(s) 58, 938
Sau3AI GATC 7 cut(s) 211, 314, 346, 511, 979, 1051, 1064
Sau96I GGNCC 1 cut(s) 24
SchI GAGTC 2 cut(s) 433, 763
ScrFI CCNGG 3 cut(s) 681, 1062, 1097
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 1 cut(s) 463
Sfr274I CTCGAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 24
SlaI CTCGAG 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
Sse9I AATT 4 cut(s) 504, 582, 663, 868
SsiI CCGC 2 cut(s) 938, 1043
SspI AATATT 1 cut(s) 88
SspMI CTAG 4 cut(s) 21, 35, 42, 542
StyD4I CCNGG 3 cut(s) 679, 1060, 1095
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 3 cut(s) 146, 164, 766
TaiI ACGT 2 cut(s) 705, 1076
TaqI TCGA 7 cut(s) 317, 394, 549, 707, 777, 872, 893
TasI AATT 4 cut(s) 504, 582, 663, 868
TatI WGTACW 1 cut(s) 137
TauI GCSGC 1 cut(s) 940
TfiI GAWTC 4 cut(s) 305, 358, 629, 890
Tru1I TTAA 4 cut(s) 288, 999, 1076, 1132
Tru9I TTAA 4 cut(s) 288, 999, 1076, 1132
TscAI CASTG 1 cut(s) 771
TseFI GTSAC 2 cut(s) 766, 815
TseI GCWGC 1 cut(s) 57
Tsp45I GTSAC 2 cut(s) 766, 815
TspDTI ATGAA 2 cut(s) 260, 911
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 1 cut(s) 771
Van91I CCANNNNNTGG 1 cut(s) 455
VpaK11BI GGWCC 1 cut(s) 24
XapI RAATTY 1 cut(s) 868
XhoI CTCGAG 1 cut(s) 393
XmaJI CCTAGG 1 cut(s) 20
XmiI GTMKAC 3 cut(s) 676, 707, 726
XspI CTAG 4 cut(s) 21, 35, 42, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.