FvH4_2g22320

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0017246
Physical Location & Seq
Forward (+)
18332981 .. 18335469
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22320.t1

Sequence Viewer

Length: 1350 bp
ATGACGCAACCACCGAACTCAACAGAAGCAGCAGAGGAAGAAAGGCAGCAGGTAGAAGAATTAGAAGAAGCAGAAGAAGATAAACATGGCAAAGGGACATGGAAGCACGCAGCGGTTCATGTGGCCACAACGATTGCCACCCCGGCTGCCTATGCTCCTCTGCCTTTCGCTCTCGCTTCTCTCGGTTGGCCTCTCGGGGTAAGCAGCTTGGTAGGTGCAACACTGGCTACATGGTATTCTAGTTTGCTCATTGCTTCTCTGTGGAAGTGGGATGGGAAGAAACATGTTACCTATCGTGACCTTGCACAAAGCATATTTGGGTTCTGGGGTTATTGGTCTATTGCATTTTTTCAGCAGGTAGCTTCTGTAGGCAATAACATTGCCATTCAAATTGCAGCTGGGAGTAGCCTTAAGGCTGTTTACAAGTATTATCACAAGGATGGTGACTTAACTCTACAGCATTTTGTTATGTTCTTTGGGATATTTGAGCTCTTTCTCTCTCAGCTCCCTGATATTCACTCCTTGAGATGGGTAAATGCCTTGTGCACTTTCAGCACCATTGGCTTTGCTGCTACAACCATTGGTGTCACAATTTATAATGGCAAAAAGATTGACAGAAAATCAATCAGTTACAGTTTAGATGGAAGCTCATCCTCCAAAATCTTTAGAGCCTTCAATGCGCTTGGAGCAATTGCTTTTTCCTTTGGAGATGCAATGCTTCCAGAGATACAGAACACTGTGAGAGAACCTGCAAAGAAGAATGTGTATAAAGGTGTATCAGCAGCTTATGGTATCATTGTGTTGAGTTACTGGCAATTGGCCTTCACCGGATACTGGGCATTTGGGTCACAAGTCCAGCCTTACATCTTGTCTTCACTAACTGTTCCTCAATGGACTATCGTCATGGCCAATATTTTTGCTGTCATACAAATATCAGGATGCTTTCAGATATATTGCAGGCCAACATATGCCTACTTTGAGGAGAGACTGTTGTCTAGTGAGACTGCTACAAAACGTGCCTTGCTACGAAACAAGCTAGTTCGTCTTGTTTGTACCTCCATGTATATGCTACTTATAACACTAGTTGCAGCAGCCATGCCATTCTTCGGGGATTTTGTGTCAATATGCGGAGCAGTTGGGTTCACACCACTGGATTTTGTGTTCCCTGCAATGGCATATCTAAAAGCTAGAAGAAGACGGCACCAAAACACCAAAATGTATCTATCTTTGCTGCTTCTTAACTTTGCTATAGTGGCTTGGTTCTCAGCTGTTGCAGTGCTCGGTTGCATTGGCGCAGTCAAGTTTATTGTGGAAGATGTCAAGACTTACAAGTTCTTTCATGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

450

Amino Acids

50.04

Weight (kDa)

9.0

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 31 - 427 1e-73 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22320
malus_domestica MD10G1191600.v1.1
prunus_persica Prupe.8G230900_v2.0.a1
pyrus_communis pycom10g16520
rosa_chinensis RchiOBHm_Chr6g0289321
rosa_laevigata RLG00000012313
rosa_multiflora Rmu_sc0001527.1_g000025 Rmu_sc0005115.1_g000002
rosa_roxburghii Rroxscaffold_7G00179340
rosa_rugosa Rorug06G0202800 Rorug06G0203000
rosa_samantha Rh6AG313600
rosa_wichuraiana Rw6G027110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 597, 1076
Acc36I ACCTGC 3 cut(s) 40, 346, 757
AccB1I GGYRCC 1 cut(s) 1200
AciI CCGC 2 cut(s) 113, 1128
AcoI YGGCCR 2 cut(s) 123, 906
AfaI GTAC 1 cut(s) 1054
AfiI CCNNNNNNNGG 4 cut(s) 528, 834, 1106, 1171
AflII CTTAAG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 2 cut(s) 389, 676
AhlI ACTAGT 1 cut(s) 1081
AloI GAACNNNNNNTCC 2 cut(s) 1145, 1177
Alw21I GWGCWC 3 cut(s) 492, 548, 1281
Alw26I GTCTC 2 cut(s) 979, 995
Alw44I GTGCAC 1 cut(s) 544
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 5 cut(s) 123, 188, 819, 906, 959
ApaLI GTGCAC 1 cut(s) 544
AspLEI GCGC 2 cut(s) 682, 1295
AsuC2I CCSGG 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 455, 817
AvaI CYCGRG 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 548
BalI TGGCCA 2 cut(s) 125, 908
BanI GGYRCC 1 cut(s) 1200
BanII GRGCYC 1 cut(s) 492
BbsI GAAGAC 2 cut(s) 864, 1201
Bbv12I GWGCWC 3 cut(s) 492, 548, 1281
BccI CCATC 4 cut(s) 266, 434, 522, 635
BceAI ACGGC 1 cut(s) 1214
BciVI GTATCC 1 cut(s) 824
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 979, 995
BcuI ACTAGT 1 cut(s) 1081
BfaI CTAG 5 cut(s) 240, 996, 1037, 1082, 1188
BfmI CTRYAG 3 cut(s) 366, 455, 1248
BfrI CTTAAG 1 cut(s) 410
BfuAI ACCTGC 3 cut(s) 40, 346, 757
BfuI GTATCC 1 cut(s) 824
BglI GCCNNNNNGGC 1 cut(s) 143
Bme1390I CCNGG 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 1202
BmrFI CCNGG 1 cut(s) 143
BmrI ACTGGG 1 cut(s) 844
BmsI GCATC 2 cut(s) 700, 929
BmuI ACTGGG 1 cut(s) 844
BoxI GACNNNNGTC 2 cut(s) 899, 991
BpiI GAAGAC 2 cut(s) 864, 1201
BpuEI CTTGAG 1 cut(s) 544
BpuMI CCSGG 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 827
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bsc4I CCNNNNNNNGG 4 cut(s) 528, 834, 1106, 1171
Bse1I ACTGG 4 cut(s) 228, 815, 839, 1155
Bse3DI GCAATG 4 cut(s) 249, 378, 720, 1176
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 4 cut(s) 277, 445, 650, 944
BseLI CCNNNNNNNGG 4 cut(s) 528, 834, 1106, 1171
BseMI GCAATG 4 cut(s) 249, 378, 720, 1176
BseMII CTCAG 2 cut(s) 515, 1278
BseNI ACTGG 4 cut(s) 228, 815, 839, 1155
BseRI GAGGAG 2 cut(s) 147, 995
BseSI GKGCMC 1 cut(s) 548
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 5 cut(s) 125, 190, 821, 908, 961
BshNI GGYRCC 1 cut(s) 1200
BsiHKAI GWGCWC 3 cut(s) 492, 548, 1281
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 2 cut(s) 143, 828
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 4 cut(s) 528, 834, 1106, 1171
BsmAI GTCTC 2 cut(s) 979, 995
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 5 cut(s) 125, 190, 821, 908, 961
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 3 cut(s) 492, 548, 1281
BspACI CCGC 2 cut(s) 113, 1128
BspANI GGCC 5 cut(s) 125, 190, 821, 908, 961
BspCNI CTCAG 2 cut(s) 514, 1277
BspHI TCATGA 1 cut(s) 1339
BspLI GGNNCC 1 cut(s) 1202
BspMI ACCTGC 3 cut(s) 40, 346, 757
BspT107I GGYRCC 1 cut(s) 1200
BspTI CTTAAG 1 cut(s) 410
BsrDI GCAATG 4 cut(s) 249, 378, 720, 1176
BsrI ACTGG 4 cut(s) 228, 815, 839, 1155
BssECI CCNNGG 1 cut(s) 141
Bst4CI ACNGT 4 cut(s) 635, 739, 883, 990
BstAFI CTTAAG 1 cut(s) 410
BstC8I GCNNGC 2 cut(s) 108, 959
BstDEI CTNAG 2 cut(s) 501, 1264
BstF5I GGATG 4 cut(s) 277, 445, 650, 944
BstHHI GCGC 2 cut(s) 682, 1295
BstMAI GTCTC 2 cut(s) 979, 995
BstMWI GCNNNNNNNGC 8 cut(s) 143, 152, 224, 552, 561, 677, 686, 1253
BstNSI RCATGY 1 cut(s) 287
BstPAI GACNNNNGTC 2 cut(s) 899, 991
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 3 cut(s) 366, 455, 1248
BstSLI GKGCMC 1 cut(s) 548
BstV2I GAAGAC 2 cut(s) 864, 1201
BsuI GTATCC 1 cut(s) 824
BsuRI GGCC 5 cut(s) 125, 190, 821, 908, 961
BtsCI GGATG 4 cut(s) 277, 445, 650, 944
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 4 cut(s) 221, 735, 1148, 1281
BveI ACCTGC 3 cut(s) 40, 346, 757
Cac8I GCNNGC 2 cut(s) 108, 959
CciI TCATGA 1 cut(s) 1339
CfoI GCGC 2 cut(s) 682, 1295
CseI GACGC 1 cut(s) 13
Csp6I GTAC 1 cut(s) 1053
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 9 cut(s) 86, 99, 119, 231, 284, 904, 1060, 1096, 1340
CviQI GTAC 1 cut(s) 1053
DdeI CTNAG 2 cut(s) 501, 1264
EaeI YGGCCR 2 cut(s) 123, 906
Ecl136II GAGCTC 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 492
FaeI CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
FaqI GGGAC 1 cut(s) 109
FatI CATG 9 cut(s) 85, 98, 118, 230, 283, 903, 1059, 1095, 1339
FauNDI CATATG 1 cut(s) 967
FokI GGATG 4 cut(s) 284, 452, 637, 951
FriOI GRGCYC 1 cut(s) 492
FspBI CTAG 5 cut(s) 240, 996, 1037, 1082, 1188
GlaI GCGC 2 cut(s) 681, 1294
GsaI CCCAGC 1 cut(s) 402
HaeIII GGCC 5 cut(s) 125, 190, 821, 908, 961
HapII CCGG 2 cut(s) 143, 828
HgaI GACGC 1 cut(s) 13
HhaI GCGC 2 cut(s) 682, 1295
Hin1II CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
Hin6I GCGC 2 cut(s) 680, 1293
HinP1I GCGC 2 cut(s) 680, 1293
HpaII CCGG 2 cut(s) 143, 828
HphI GGTGA 2 cut(s) 455, 817
Hpy166II GTNNAC 3 cut(s) 421, 546, 1143
Hpy188I TCNGA 1 cut(s) 948
Hpy188III TCNNGA 5 cut(s) 296, 722, 936, 1321, 1340
Hpy8I GTNNAC 3 cut(s) 421, 546, 1143
HpyAV CCTTC 2 cut(s) 682, 832
HpyCH4III ACNGT 4 cut(s) 635, 739, 883, 990
HpyCH4IV ACGT 1 cut(s) 1015
HpyF10VI GCNNNNNNNGC 8 cut(s) 143, 152, 224, 552, 561, 677, 686, 1253
HpyF3I CTNAG 2 cut(s) 501, 1264
HpySE526I ACGT 1 cut(s) 1015
Hsp92II CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
HspAI GCGC 2 cut(s) 680, 1293
LmnI GCTCC 4 cut(s) 160, 510, 686, 1130
LweI GCATC 2 cut(s) 700, 929
MaeI CTAG 5 cut(s) 240, 996, 1037, 1082, 1188
MaeII ACGT 1 cut(s) 1015
MaeIII GTNAC 7 cut(s) 286, 296, 443, 586, 629, 806, 846
MfeI CAATTG 2 cut(s) 690, 815
MhlI GDGCHC 3 cut(s) 492, 548, 1281
MlsI TGGCCA 2 cut(s) 125, 908
MluCI AATT 5 cut(s) 59, 390, 591, 690, 815
MluNI TGGCCA 2 cut(s) 125, 908
MnlI CCTC 7 cut(s) 28, 168, 201, 664, 897, 973, 1066
Mox20I TGGCCA 2 cut(s) 125, 908
MscI TGGCCA 2 cut(s) 125, 908
MseI TTAA 3 cut(s) 411, 449, 1239
MslI CAYNNNNRTG 3 cut(s) 438, 1064, 1214
Msp20I TGGCCA 2 cut(s) 125, 908
MspA1I CMGCKG 3 cut(s) 113, 398, 1268
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 2 cut(s) 143, 828
MspR9I CCNGG 1 cut(s) 143
MunI CAATTG 2 cut(s) 690, 815
MwoI GCNNNNNNNGC 8 cut(s) 143, 152, 224, 552, 561, 677, 686, 1253
NciI CCSGG 1 cut(s) 143
NdeI CATATG 1 cut(s) 967
NlaIII CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
NlaIV GGNNCC 1 cut(s) 1202
NmuCI GTSAC 4 cut(s) 296, 443, 586, 846
NspI RCATGY 1 cut(s) 287
PagI TCATGA 1 cut(s) 1339
PciI ACATGT 1 cut(s) 283
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PscI ACATGT 1 cut(s) 283
PshAI GACNNNNGTC 2 cut(s) 899, 991
PsiI TTATAA 2 cut(s) 597, 1076
Psp124BI GAGCTC 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 1202
PvuII CAGCTG 2 cut(s) 398, 1268
RsaI GTAC 1 cut(s) 1054
RsaNI GTAC 1 cut(s) 1053
RseI CAYNNNNRTG 3 cut(s) 438, 1064, 1214
SacI GAGCTC 1 cut(s) 492
SaqAI TTAA 3 cut(s) 411, 449, 1239
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 3 cut(s) 492, 548, 1281
SfaNI GCATC 2 cut(s) 700, 929
SfcI CTRYAG 3 cut(s) 366, 455, 1248
SmiMI CAYNNNNRTG 3 cut(s) 438, 1064, 1214
SmlI CTYRAG 2 cut(s) 410, 523
SmoI CTYRAG 2 cut(s) 410, 523
SpeI ACTAGT 1 cut(s) 1081
Sse9I AATT 5 cut(s) 59, 390, 591, 690, 815
SsiI CCGC 2 cut(s) 113, 1128
SspI AATATT 1 cut(s) 913
SspMI CTAG 5 cut(s) 240, 996, 1037, 1082, 1188
SstI GAGCTC 1 cut(s) 492
StyD4I CCNGG 1 cut(s) 141
TaaI ACNGT 4 cut(s) 635, 739, 883, 990
TaiI ACGT 1 cut(s) 1018
TasI AATT 5 cut(s) 59, 390, 591, 690, 815
Tru1I TTAA 3 cut(s) 411, 449, 1239
Tru9I TTAA 3 cut(s) 411, 449, 1239
TscAI CASTG 4 cut(s) 228, 742, 1155, 1281
TseFI GTSAC 4 cut(s) 296, 443, 586, 846
Tsp45I GTSAC 4 cut(s) 296, 443, 586, 846
TspDTI ATGAA 2 cut(s) 107, 1328
TspRI CASTG 4 cut(s) 228, 742, 1155, 1281
Vha464I CTTAAG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 544
XceI RCATGY 1 cut(s) 287
XspI CTAG 5 cut(s) 240, 996, 1037, 1082, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.