RLG00000012313

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0017246
Physical Location & Seq
Reverse (-)
17402665 .. 17404816
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012313

Sequence Viewer

Length: 1350 bp
ATGACACAACCACCAGATTCAACAGAAGCAGCAGAGGAAGAAAGGCAGCAAGTAGAAGAAGTAGAAGAGGCAGAAGAATCAAAACATGGCAAAGGGACATGGAAGCACGCAGCGTTTCATGTGGCCACAACGATTGCCACACCTGCTGCCTATGCTCCTCTGCCTTTTGCTCTCGCTTCTCTTGGTTGGCCTCTCGGGGTAAGCAGCTTGGTAGGTGCAACACTGGCCACATGGTATTCTAGTCTGCTCATCGCTTCTCTGTGGAAGTGGGATGGGAAGAAACATGTTACCTATCGCCACCTTGCCCAAAGCATTTTCGGGTTCTGGGGTTATTGGTCTATTGCATTTTTTCAGCAGGTAGCTTCAATAGGCAATAACATTGCCATTCAAATTGCAGCTGGGAGTAGCCTCAAGGCTGTTTACAAGTATTATCACAAGGATGGTGACTTAACTCTGCAACATTTTGTTATTTTCTTTGGGGTCTTTGAGCTCCTTCTCTCTCAGCTCCCTGATATACACTCCTTAAGATGGGTAAATGCCTTGTGCACTTTCAGCACAATTGGCTTTGCTGCTACAACCATAGGTGTCACAATTTATAATGGGAAAAAGATTGACAGAAAATCAATCAGTTACGGTTTAGATGGAAGCTCATCATCCAAAGTCTTTAGAGCCTTCAATGCTCTCGGAGCAATTGCCTTTTCCTTTGGAGATGCAATGCTTCCAGAGATACAGAACACTGTGAAAGAACCTGCAAAGAAAAATGTGTACAAAGGTGTATCAGCAGCTTATGGTATCATAGTGTTGAGTTACTGGCAATTGGCCTTCACTGGGTACTGGGCATTTGGGTCACAAGTTAAGCCTTACATCTTGTCTTCACTTACTGTTCCTCAATGGACTATTGTCATGGCCAATCTTTTTGCTGTCATACAAATATCAGGATGCTTTCAGATTTACTGCAGGCCAACATATGCCTACTTCGAGGAGAGACTTTTGTCTAATGAAACTGCTACAAGTCGTTCCTCACTAAGAAACAAGCTAGTTCGTCTTGTTTGTACCTCCATGTATATGCTACTTATTACACTAGTTGCAGCAGCCATGCCATTCTTTGGGGATTTTGTGGCAATATGCGGAGCAATTGGGTTCACACCACTGGACTTTGTGTTCCCTGCTATGGCATATCTAAAAGCTAGAAGAAGACGGCACCAAAACACCAAAATGTATCTGTCTTTGCTACTTCTTAACTTTGCCATAGTGATTTGGTTCTCGGCTGTTGCAGTGTTCGGTTGCATTGGGGCAGTCAAGTTTATTGTGGAAGATGTCAAGACTTATAAGTTCTTTCATGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

450

Amino Acids

50.0

Weight (kDa)

9.07

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 31 - 431 1.9e-74 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22320
malus_domestica MD10G1191600.v1.1
prunus_persica Prupe.8G230900_v2.0.a1
pyrus_communis pycom10g16520
rosa_chinensis RchiOBHm_Chr6g0289321
rosa_laevigata RLG00000012313
rosa_multiflora Rmu_sc0001527.1_g000025 Rmu_sc0005115.1_g000002
rosa_roxburghii Rroxscaffold_7G00179340
rosa_rugosa Rorug06G0202800 Rorug06G0203000
rosa_samantha Rh6AG313600
rosa_wichuraiana Rw6G027110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 597, 1329
AarI CACCTGC 1 cut(s) 151
Acc36I ACCTGC 3 cut(s) 151, 346, 757
AccB1I GGYRCC 1 cut(s) 1200
AccB7I CCANNNNNTGG 1 cut(s) 1106
AciI CCGC 1 cut(s) 1128
AcoI YGGCCR 3 cut(s) 123, 225, 906
AfaI GTAC 3 cut(s) 767, 833, 1054
AfiI CCNNNNNNNGG 4 cut(s) 528, 828, 1106, 1171
AflII CTTAAG 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 283
AgsI TTSAA 4 cut(s) 21, 366, 389, 676
AhlI ACTAGT 1 cut(s) 1081
AloI GAACNNNNNNTCC 2 cut(s) 1145, 1177
AluBI AGCT 9 cut(s) 207, 362, 398, 490, 505, 648, 785, 1036, 1187
AluI AGCT 9 cut(s) 207, 362, 398, 490, 505, 648, 785, 1036, 1187
Alw21I GWGCWC 2 cut(s) 492, 548
Alw26I GTCTC 1 cut(s) 979
Alw44I GTGCAC 1 cut(s) 544
Ama87I CYCGRG 1 cut(s) 194
AoxI GGCC 6 cut(s) 123, 188, 225, 819, 906, 959
ApaLI GTGCAC 1 cut(s) 544
AsuHPI GGTGA 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 548
BalI TGGCCA 3 cut(s) 125, 227, 908
BanI GGYRCC 1 cut(s) 1200
BanII GRGCYC 1 cut(s) 492
BbsI GAAGAC 2 cut(s) 864, 1201
Bbv12I GWGCWC 2 cut(s) 492, 548
BccI CCATC 4 cut(s) 266, 434, 522, 635
BceAI ACGGC 1 cut(s) 1214
BcoDI GTCTC 1 cut(s) 979
BcuI ACTAGT 1 cut(s) 1081
BfaI CTAG 4 cut(s) 240, 1037, 1082, 1188
BfmI CTRYAG 1 cut(s) 955
BfrI CTTAAG 1 cut(s) 523
BfuAI ACCTGC 3 cut(s) 151, 346, 757
BmeT110I CYCGRG 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 1202
BmrI ACTGGG 2 cut(s) 837, 844
BmsI GCATC 2 cut(s) 700, 929
BmuI ACTGGG 2 cut(s) 837, 844
BoxI GACNNNNGTC 2 cut(s) 899, 991
BpiI GAAGAC 2 cut(s) 864, 1201
BpuEI CTTGAG 1 cut(s) 395
Bsc4I CCNNNNNNNGG 4 cut(s) 528, 828, 1106, 1171
Bse1I ACTGG 5 cut(s) 228, 815, 832, 839, 1155
Bse3DI GCAATG 2 cut(s) 378, 720
BseGI GGATG 4 cut(s) 277, 445, 653, 944
BseLI CCNNNNNNNGG 4 cut(s) 528, 828, 1106, 1171
BseMI GCAATG 2 cut(s) 378, 720
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 5 cut(s) 228, 815, 832, 839, 1155
BseRI GAGGAG 2 cut(s) 147, 995
BseSI GKGCMC 1 cut(s) 548
BseYI CCCAGC 1 cut(s) 398
BshFI GGCC 6 cut(s) 125, 190, 227, 821, 908, 961
BshNI GGYRCC 1 cut(s) 1200
BsiHKAI GWGCWC 2 cut(s) 492, 548
BsiHKCI CYCGRG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 4 cut(s) 528, 828, 1106, 1171
BsmAI GTCTC 1 cut(s) 979
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 6 cut(s) 125, 190, 227, 821, 908, 961
BsoBI CYCGRG 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 492, 548
Bsp1407I TGTACA 1 cut(s) 765
BspACI CCGC 1 cut(s) 1128
BspANI GGCC 6 cut(s) 125, 190, 227, 821, 908, 961
BspCNI CTCAG 1 cut(s) 514
BspHI TCATGA 1 cut(s) 1339
BspLI GGNNCC 1 cut(s) 1202
BspMAI CTGCAG 1 cut(s) 959
BspMI ACCTGC 3 cut(s) 151, 346, 757
BspT107I GGYRCC 1 cut(s) 1200
BspTI CTTAAG 1 cut(s) 523
BsrDI GCAATG 2 cut(s) 378, 720
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 5 cut(s) 228, 815, 832, 839, 1155
Bst4CI ACNGT 3 cut(s) 635, 739, 883
Bst6I CTCTTC 1 cut(s) 60
BstAFI CTTAAG 1 cut(s) 523
BstAUI TGTACA 1 cut(s) 765
BstC8I GCNNGC 2 cut(s) 108, 959
BstDEI CTNAG 2 cut(s) 501, 1025
BstF5I GGATG 4 cut(s) 277, 445, 653, 944
BstMAI GTCTC 1 cut(s) 979
BstMWI GCNNNNNNNGC 7 cut(s) 143, 152, 224, 552, 561, 677, 686
BstNSI RCATGY 1 cut(s) 287
BstPAI GACNNNNGTC 2 cut(s) 899, 991
BstSFI CTRYAG 1 cut(s) 955
BstSLI GKGCMC 1 cut(s) 548
BstV2I GAAGAC 2 cut(s) 864, 1201
BsuRI GGCC 6 cut(s) 125, 190, 227, 821, 908, 961
BtgZI GCGATG 1 cut(s) 235
BtsCI GGATG 4 cut(s) 277, 445, 653, 944
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 5 cut(s) 221, 735, 825, 1148, 1281
BveI ACCTGC 3 cut(s) 151, 346, 757
Cac8I GCNNGC 2 cut(s) 108, 959
CciI TCATGA 1 cut(s) 1339
Csp6I GTAC 3 cut(s) 766, 832, 1053
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviAII CATG 9 cut(s) 86, 99, 119, 231, 284, 904, 1060, 1096, 1340
CviQI GTAC 3 cut(s) 766, 832, 1053
DdeI CTNAG 2 cut(s) 501, 1025
EaeI YGGCCR 3 cut(s) 123, 225, 906
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 194
EcoICRI GAGCTC 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 492
FaeI CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
FaqI GGGAC 1 cut(s) 109
FatI CATG 9 cut(s) 85, 98, 118, 230, 283, 903, 1059, 1095, 1339
FauNDI CATATG 1 cut(s) 967
FokI GGATG 4 cut(s) 284, 452, 640, 951
FriOI GRGCYC 1 cut(s) 492
FspBI CTAG 4 cut(s) 240, 1037, 1082, 1188
GsaI CCCAGC 1 cut(s) 402
HaeIII GGCC 6 cut(s) 125, 190, 227, 821, 908, 961
Hin1II CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
HinfI GANTC 2 cut(s) 17, 77
HphI GGTGA 1 cut(s) 455
Hpy166II GTNNAC 4 cut(s) 421, 546, 766, 1143
Hpy188I TCNGA 2 cut(s) 686, 948
Hpy188III TCNNGA 4 cut(s) 722, 936, 1321, 1340
Hpy8I GTNNAC 4 cut(s) 421, 546, 766, 1143
HpyAV CCTTC 3 cut(s) 503, 682, 832
HpyCH4III ACNGT 3 cut(s) 635, 739, 883
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 152, 224, 552, 561, 677, 686
HpyF3I CTNAG 2 cut(s) 501, 1025
Hsp92II CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
LmnI GCTCC 5 cut(s) 160, 495, 510, 686, 1130
LweI GCATC 2 cut(s) 700, 929
MaeI CTAG 4 cut(s) 240, 1037, 1082, 1188
MaeIII GTNAC 6 cut(s) 286, 443, 586, 629, 806, 846
MboII GAAGA 9 cut(s) 50, 68, 77, 86, 289, 864, 1203, 1206, 1325
MfeI CAATTG 4 cut(s) 558, 690, 815, 1134
MhlI GDGCHC 2 cut(s) 492, 548
MlsI TGGCCA 3 cut(s) 125, 227, 908
MluCI AATT 6 cut(s) 390, 558, 591, 690, 815, 1134
MluNI TGGCCA 3 cut(s) 125, 227, 908
MnlI CCTC 9 cut(s) 28, 61, 168, 201, 419, 897, 973, 1030, 1066
Mox20I TGGCCA 3 cut(s) 125, 227, 908
MscI TGGCCA 3 cut(s) 125, 227, 908
MseI TTAA 4 cut(s) 449, 524, 855, 1239
MslI CAYNNNNRTG 3 cut(s) 438, 1064, 1214
Msp20I TGGCCA 3 cut(s) 125, 227, 908
MspA1I CMGCKG 1 cut(s) 398
MspCI CTTAAG 1 cut(s) 523
MunI CAATTG 4 cut(s) 558, 690, 815, 1134
MwoI GCNNNNNNNGC 7 cut(s) 143, 152, 224, 552, 561, 677, 686
NdeI CATATG 1 cut(s) 967
NlaIII CATG 9 cut(s) 89, 102, 122, 234, 287, 907, 1063, 1099, 1343
NlaIV GGNNCC 1 cut(s) 1202
NmeAIII GCCGAG 1 cut(s) 1244
NmuCI GTSAC 3 cut(s) 443, 586, 846
NspI RCATGY 1 cut(s) 287
PagI TCATGA 1 cut(s) 1339
PaqCI CACCTGC 1 cut(s) 151
PciI ACATGT 1 cut(s) 283
PfeI GAWTC 2 cut(s) 17, 77
PflMI CCANNNNNTGG 1 cut(s) 1106
PscI ACATGT 1 cut(s) 283
PshAI GACNNNNGTC 2 cut(s) 899, 991
PsiI TTATAA 2 cut(s) 597, 1329
Psp124BI GAGCTC 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 1202
PstI CTGCAG 1 cut(s) 959
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 3 cut(s) 767, 833, 1054
RsaNI GTAC 3 cut(s) 766, 832, 1053
RseI CAYNNNNRTG 3 cut(s) 438, 1064, 1214
SacI GAGCTC 1 cut(s) 492
SaqAI TTAA 4 cut(s) 449, 524, 855, 1239
SduI GDGCHC 2 cut(s) 492, 548
SfaNI GCATC 2 cut(s) 700, 929
SfcI CTRYAG 1 cut(s) 955
SmiMI CAYNNNNRTG 3 cut(s) 438, 1064, 1214
SmlI CTYRAG 2 cut(s) 410, 523
SmoI CTYRAG 2 cut(s) 410, 523
SpeI ACTAGT 1 cut(s) 1081
Sse9I AATT 6 cut(s) 390, 558, 591, 690, 815, 1134
SsiI CCGC 1 cut(s) 1128
SspMI CTAG 4 cut(s) 240, 1037, 1082, 1188
SstI GAGCTC 1 cut(s) 492
TaaI ACNGT 3 cut(s) 635, 739, 883
TaqI TCGA 1 cut(s) 978
TasI AATT 6 cut(s) 390, 558, 591, 690, 815, 1134
TatI WGTACW 1 cut(s) 765
TfiI GAWTC 2 cut(s) 17, 77
Tru1I TTAA 4 cut(s) 449, 524, 855, 1239
Tru9I TTAA 4 cut(s) 449, 524, 855, 1239
TscAI CASTG 5 cut(s) 228, 742, 832, 1155, 1281
TseFI GTSAC 3 cut(s) 443, 586, 846
Tsp45I GTSAC 3 cut(s) 443, 586, 846
TspDTI ATGAA 3 cut(s) 107, 1014, 1328
TspRI CASTG 5 cut(s) 228, 742, 832, 1155, 1281
Van91I CCANNNNNTGG 1 cut(s) 1106
Vha464I CTTAAG 1 cut(s) 523
VneI GTGCAC 1 cut(s) 544
XceI RCATGY 1 cut(s) 287
XspI CTAG 4 cut(s) 240, 1037, 1082, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.