Rorug06G0203000

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017246
Physical Location & Seq
Forward (+)
32517123 .. 32519176
2054 bp
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UTR
Exon/CDS
Intron
Rorug06G0203000.1

Sequence Viewer

Length: 657 bp
ATGAAATCTCTCTACTATAAGTTTCTATGTTTGGTCGTACTGAGCTTCTTCAACAACCACCATTTGGGTAGTGCTGATCCTACAGATGGGTTCACCCTTGTGCCATTAACAGATCGGAACTTTAAGCTACAAAAACCATATAACGAACCCCTTGATGATCGATACAGCTACAAAGATGGGGTTCGAAGTTTCTGGATATACAACAATGACAAGCCCTTCAAAACAGATAGCACAACCAGGCCACGCTCGGAACTACGCATAACGGGACATGACTATTCTTCTGGAATTTGGCAATTTGAAGGCTATGCATATGTGCCCAGTGGTACTTCTGGGGTTACAATAGTGCAGATTCATGGTGCAACTGAAGGAGCTACAACTCTACAACTAAGGATGTATGAAGGAGACGGTGGAGATCTCAGATACTACAGATACAACTTAGTTGATACTGATCTCTACGACAAGTGGTTCAGAGTAAACATAATCCACAATGTTGACAAAGGGAAGGTCATAGTTTTCATTGACGGTGTTAAGAAGTTTGTGGTGAAAGATCAGGGACCAGGGGACCTGTACTTCAAATGTGGTGTGTATGCAGCACCGTTTAATTCCAGTAACTACATGGAATCAAGGTGGAAGGAGATCAAACTTTACAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

218

Amino Acids

25.3

Weight (kDa)

8.82

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 39 - 216 2.4e-29 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22320
malus_domestica MD10G1191600.v1.1
prunus_persica Prupe.8G230900_v2.0.a1
pyrus_communis pycom10g16520
rosa_chinensis RchiOBHm_Chr6g0289321
rosa_laevigata RLG00000012313
rosa_multiflora Rmu_sc0001527.1_g000025 Rmu_sc0005115.1_g000002
rosa_roxburghii Rroxscaffold_7G00179340
rosa_rugosa Rorug06G0202800 Rorug06G0203000
rosa_samantha Rh6AG313600
rosa_wichuraiana Rw6G027110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 64
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 1 cut(s) 285
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 39, 325, 569
AfiI CCNNNNNNNGG 2 cut(s) 64, 86
AgsI TTSAA 4 cut(s) 52, 220, 299, 574
AjnI CCWGG 2 cut(s) 236, 556
AleI CACNNNNGTG 1 cut(s) 98
AluBI AGCT 4 cut(s) 45, 127, 168, 371
AluI AGCT 4 cut(s) 45, 127, 168, 371
Alw26I GTCTC 1 cut(s) 396
AlwI GGATC 1 cut(s) 71
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 1 cut(s) 590
ApoI RAATTY 1 cut(s) 285
ArsI GACNNNNNNTTYG 2 cut(s) 489, 521
AspS9I GGNCC 2 cut(s) 554, 562
AsuHPI GGTGA 2 cut(s) 85, 553
AsuII TTCGAA 1 cut(s) 184
AvaII GGWCC 2 cut(s) 554, 562
BaeGI GKGCMC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 602
BccI CCATC 2 cut(s) 80, 170
BciT130I CCWGG 2 cut(s) 238, 558
BcoDI GTCTC 1 cut(s) 396
BfmI CTRYAG 2 cut(s) 81, 424
BglII AGATCT 1 cut(s) 412
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
Bme1390I CCNGG 2 cut(s) 238, 558
Bme18I GGWCC 2 cut(s) 554, 562
BmgT120I GGNCC 2 cut(s) 554, 562
BmiI GGNNCC 2 cut(s) 555, 563
BmrFI CCNGG 2 cut(s) 238, 558
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
Bpu14I TTCGAA 1 cut(s) 184
Bsa29I ATCGAT 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 557
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 86
Bse1I ACTGG 2 cut(s) 318, 606
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 2 cut(s) 238, 558
BseCI ATCGAT 1 cut(s) 160
BseDI CCNNGG 1 cut(s) 557
BseGI GGATG 1 cut(s) 396
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 2 cut(s) 64, 86
BseMII CTCAG 2 cut(s) 32, 430
BseNI ACTGG 2 cut(s) 318, 606
BseSI GKGCMC 1 cut(s) 318
BseXI GCAGC 1 cut(s) 602
BsgI GTGCAG 1 cut(s) 365
BshFI GGCC 1 cut(s) 241
BshVI ATCGAT 1 cut(s) 160
BslFI GGGAC 3 cut(s) 279, 567, 575
BslI CCNNNNNNNGG 2 cut(s) 64, 86
BsmAI GTCTC 1 cut(s) 396
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 3 cut(s) 279, 567, 575
BsnI GGCC 1 cut(s) 241
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 2 cut(s) 33, 429
BspDI ATCGAT 1 cut(s) 160
BspLI GGNNCC 2 cut(s) 555, 563
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 184
BsrI ACTGG 2 cut(s) 318, 606
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
Bst2UI CCWGG 2 cut(s) 238, 558
Bst4CI ACNGT 3 cut(s) 407, 524, 597
BstBI TTCGAA 1 cut(s) 184
BstDEI CTNAG 4 cut(s) 41, 386, 416, 436
BstF5I GGATG 1 cut(s) 396
BstKTI GATC 7 cut(s) 79, 115, 160, 415, 451, 550, 639
BstMAI GTCTC 1 cut(s) 396
BstMBI GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
BstNI CCWGG 2 cut(s) 238, 558
BstSCI CCNGG 2 cut(s) 236, 556
BstSFI CTRYAG 2 cut(s) 81, 424
BstSLI GKGCMC 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
Bsu15I ATCGAT 1 cut(s) 160
BsuRI GGCC 1 cut(s) 241
BsuTUI ATCGAT 1 cut(s) 160
BtsCI GGATG 1 cut(s) 396
BtsIMutI CAGTG 1 cut(s) 325
Cfr13I GGNCC 2 cut(s) 554, 562
ClaI ATCGAT 1 cut(s) 160
Csp6I GTAC 3 cut(s) 38, 324, 568
CviAII CATG 3 cut(s) 269, 353, 616
CviJI RGCY 7 cut(s) 45, 127, 168, 214, 241, 303, 371
CviKI_1 RGCY 7 cut(s) 45, 127, 168, 214, 241, 303, 371
CviQI GTAC 3 cut(s) 38, 324, 568
DdeI CTNAG 4 cut(s) 41, 386, 416, 436
DpnI GATC 7 cut(s) 78, 114, 159, 414, 450, 549, 638
DpnII GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
Eco47I GGWCC 2 cut(s) 554, 562
Eco57I CTGAAG 1 cut(s) 384
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 2 cut(s) 236, 556
EcoT22I ATGCAT 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 3 cut(s) 272, 356, 619
FaqI GGGAC 3 cut(s) 279, 567, 575
FatI CATG 3 cut(s) 268, 352, 615
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 1 cut(s) 403
Fsp4HI GCNGC 1 cut(s) 591
GluI GCNGC 1 cut(s) 591
HaeIII GGCC 1 cut(s) 241
Hin1II CATG 3 cut(s) 272, 356, 619
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 2 cut(s) 349, 620
HphI GGTGA 2 cut(s) 85, 553
Hpy166II GTNNAC 3 cut(s) 93, 475, 493
Hpy188I TCNGA 4 cut(s) 117, 250, 419, 470
Hpy188III TCNNGA 2 cut(s) 193, 282
Hpy8I GTNNAC 3 cut(s) 93, 475, 493
HpyAV CCTTC 6 cut(s) 226, 293, 359, 392, 496, 625
HpyCH4III ACNGT 3 cut(s) 407, 524, 597
HpyCH4V TGCA 4 cut(s) 308, 346, 359, 590
HpyF3I CTNAG 4 cut(s) 41, 386, 416, 436
Hsp92II CATG 3 cut(s) 272, 356, 619
Kzo9I GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
LmnI GCTCC 1 cut(s) 368
Lsp1109I GCAGC 1 cut(s) 602
MaeIII GTNAC 2 cut(s) 334, 608
MalI GATC 7 cut(s) 78, 114, 159, 414, 450, 549, 638
MboI GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
MboII GAAGA 2 cut(s) 40, 270
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 3 cut(s) 285, 293, 601
Mph1103I ATGCAT 1 cut(s) 310
MseI TTAA 4 cut(s) 107, 123, 528, 600
MslI CAYNNNNRTG 1 cut(s) 98
MspR9I CCNGG 2 cut(s) 238, 558
MvaI CCWGG 2 cut(s) 238, 558
NdeI CATATG 1 cut(s) 310
NdeII GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
NlaIII CATG 3 cut(s) 272, 356, 619
NlaIV GGNNCC 2 cut(s) 555, 563
NsiI ATGCAT 1 cut(s) 310
NspV TTCGAA 1 cut(s) 184
OliI CACNNNNGTG 1 cut(s) 98
PfeI GAWTC 2 cut(s) 349, 620
PflMI CCANNNNNTGG 1 cut(s) 64
PkrI GCNGC 1 cut(s) 592
PpuMI RGGWCCY 1 cut(s) 562
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 2 cut(s) 236, 556
PspGI CCWGG 2 cut(s) 236, 556
PspN4I GGNNCC 2 cut(s) 555, 563
PspPI GGNCC 2 cut(s) 554, 562
PspPPI RGGWCCY 1 cut(s) 562
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 3 cut(s) 39, 325, 569
RsaNI GTAC 3 cut(s) 38, 324, 568
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 4 cut(s) 107, 123, 528, 600
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 7 cut(s) 76, 112, 157, 412, 448, 547, 636
Sau96I GGNCC 2 cut(s) 554, 562
ScrFI CCNGG 2 cut(s) 238, 558
SduI GDGCHC 1 cut(s) 318
SetI ASST 7 cut(s) 47, 129, 170, 373, 507, 567, 629
SfcI CTRYAG 2 cut(s) 81, 424
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 2 cut(s) 554, 562
SmiMI CAYNNNNRTG 1 cut(s) 98
Sse9I AATT 3 cut(s) 285, 293, 601
StyD4I CCNGG 2 cut(s) 236, 556
TaaI ACNGT 3 cut(s) 407, 524, 597
TaqI TCGA 2 cut(s) 160, 184
TasI AATT 3 cut(s) 285, 293, 601
TatI WGTACW 1 cut(s) 567
TfiI GAWTC 2 cut(s) 349, 620
Tru1I TTAA 4 cut(s) 107, 123, 528, 600
Tru9I TTAA 4 cut(s) 107, 123, 528, 600
TscAI CASTG 1 cut(s) 325
TseI GCWGC 1 cut(s) 590
TspDTI ATGAA 4 cut(s) 17, 341, 411, 505
TspRI CASTG 1 cut(s) 325
Van91I CCANNNNNTGG 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 554, 562
XapI RAATTY 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.