RchiOBHm_Chr6g0289321

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017246
Physical Location & Seq
Forward (+)
52422229 .. 52424469
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25955

Sequence Viewer

Length: 1392 bp
ATGAAGCTGCACGTATTTGCAAGTTTAAGGTTTAGAAGCAAAATGACACAACCACCAGATTCAGCAGAAGCAGCAGAGGAAGAAAGGCAGCAAGTAGAAGAAGTAGAAGAGGCAGAAGAATCAAAACATGGCAAAGGGACATGGAAGCACGCAGCGTTTCATGTGGCCACAACGATTGCCACACCTGCTGCCTATTCTCCTCTGCCTTTTGCTCTCGCTTCTCTTGGTTGGTCTCTCGGGGTAAGCAGCTTGGTAGGTGCAACACTGGCCACATGGTATTCTAGTCTGCTTATCGCTTCTCTGTGGAAGTGGGATGGGAAGAAACATGTTACCTATCGCCACCTTGCCCAAAGCATTTTCGGGTTCTGGGGTTATTGGTCTATTGCATTTTTTCAGCAGGTAGCTTCAATAGGCAATAACATTGCCATTCAAATTGCAGCTGGGAGTAGCCTTAAGGCTGTTTACAAGTATTATCACAAGGATGGTGACTTAACTCTGCAACATTTTGTTATTTTCTTTGGGGTCTTTGAGCTCTTTCTCTCTCAGCTCCCTGATATACACTCCTTAAGATGGGTAAATGCCTTGTGCACTTTCAGCACCATTGGCTTTGCTGCTACAACCATTGGTGTCACAATTTATAATGGGAAAAAGATTGACAGAAAATCAATCAGTTACGGTTTAGATGGAAGCTCATCATCCAAAGTCTTTAGAGCCTTCAATGCTCTCGGAGCAATTGCCTTTTCCTTTGGAGATGCAATGCTTCCAGAGATACAGAACACTGTGAGAGAACCTGCAAAGAAAAATGTGTACAAAGGTGTATCAGCAGCTTATGGTATCATAGTGTTGAGTTACTGGCAATTGGCCTTCACTGGGTACTGGGCTTTTGGGTCACAAGTTGAGCCTTACATCTTGTCTTCACTCACTGTTCCTCAATGGACTATTGTCATGGCCAATCTTTTTGCTGTCATACAAATATCAGGATGCTTTCAGATATACTGCAGGCCAACATATGCCTACTTCGAGGAGACACTGTTGTCTAATGAAACTGCCACAAGTCGTTCCTCACTAAGAAACAAGCTAGTTCGTCTTGTTTGTACCTCCATGTATATGCTACTTATTACACTAGTTGCAGCAGCCATGCCATTCTTTGGGGATTTTGTGGCAATATGCGGAGCAATTGGGTTCACACCACTGGACTTTGTGTTCCCTGCTATGGCATATCTAAAAGCTAGAAGAAGACGGCACCAAAACACCAAAATGTATCTGTCTTTGCTACTTCTTAACTTTGCTATAGTGGTGTGGTTCTCGGCTGTTGCAGTGTTCGGTTGCATTGGGGCAGTCAAGTTTATTGTGGAAGATGTCAAGACTTATAAGTTCTTTCATGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

463

Amino Acids

51.67

Weight (kDa)

9.14

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 45 - 445 6.2e-74 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22320
malus_domestica MD10G1191600.v1.1
prunus_persica Prupe.8G230900_v2.0.a1
pyrus_communis pycom10g16520
rosa_chinensis RchiOBHm_Chr6g0289321
rosa_laevigata RLG00000012313
rosa_multiflora Rmu_sc0001527.1_g000025 Rmu_sc0005115.1_g000002
rosa_roxburghii Rroxscaffold_7G00179340
rosa_rugosa Rorug06G0202800 Rorug06G0203000
rosa_samantha Rh6AG313600
rosa_wichuraiana Rw6G027110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 639, 1371
AarI CACCTGC 1 cut(s) 193
AasI GACNNNNNNGTC 1 cut(s) 1033
Acc36I ACCTGC 3 cut(s) 193, 388, 799
AccB1I GGYRCC 1 cut(s) 1242
AccB7I CCANNNNNTGG 1 cut(s) 1148
AciI CCGC 1 cut(s) 1170
AcoI YGGCCR 3 cut(s) 165, 267, 948
AfaI GTAC 3 cut(s) 809, 875, 1096
AfiI CCNNNNNNNGG 4 cut(s) 570, 870, 1148, 1213
AflII CTTAAG 2 cut(s) 452, 565
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 3 cut(s) 408, 431, 718
AhlI ACTAGT 1 cut(s) 1123
AloI GAACNNNNNNTCC 2 cut(s) 1187, 1219
Alw21I GWGCWC 2 cut(s) 534, 590
Alw26I GTCTC 2 cut(s) 237, 1019
Alw44I GTGCAC 1 cut(s) 586
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 5 cut(s) 165, 267, 861, 948, 1001
ApaLI GTGCAC 1 cut(s) 586
AsuHPI GGTGA 1 cut(s) 497
AvaI CYCGRG 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 590
BalI TGGCCA 3 cut(s) 167, 269, 950
BanI GGYRCC 1 cut(s) 1242
BanII GRGCYC 1 cut(s) 534
BbsI GAAGAC 2 cut(s) 906, 1243
Bbv12I GWGCWC 2 cut(s) 534, 590
BccI CCATC 4 cut(s) 308, 476, 564, 677
BceAI ACGGC 1 cut(s) 1256
BcoDI GTCTC 2 cut(s) 237, 1019
BcuI ACTAGT 1 cut(s) 1123
BfaI CTAG 4 cut(s) 282, 1079, 1124, 1230
BfmI CTRYAG 2 cut(s) 997, 1290
BfrI CTTAAG 2 cut(s) 452, 565
BfuAI ACCTGC 3 cut(s) 193, 388, 799
BmeT110I CYCGRG 1 cut(s) 236
BmiI GGNNCC 1 cut(s) 1244
BmrI ACTGGG 2 cut(s) 879, 886
BmsI GCATC 2 cut(s) 742, 971
BmuI ACTGGG 2 cut(s) 879, 886
BoxI GACNNNNGTC 1 cut(s) 941
BpiI GAAGAC 2 cut(s) 906, 1243
BsaAI YACGTR 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 1016, 1046
Bsc4I CCNNNNNNNGG 4 cut(s) 570, 870, 1148, 1213
Bse1I ACTGG 5 cut(s) 270, 857, 874, 881, 1197
Bse3DI GCAATG 2 cut(s) 420, 762
BseGI GGATG 4 cut(s) 319, 487, 695, 986
BseLI CCNNNNNNNGG 4 cut(s) 570, 870, 1148, 1213
BseMI GCAATG 2 cut(s) 420, 762
BseMII CTCAG 1 cut(s) 557
BseNI ACTGG 5 cut(s) 270, 857, 874, 881, 1197
BseRI GAGGAG 2 cut(s) 189, 1037
BseSI GKGCMC 1 cut(s) 590
BseYI CCCAGC 1 cut(s) 440
BshFI GGCC 5 cut(s) 167, 269, 863, 950, 1003
BshNI GGYRCC 1 cut(s) 1242
BsiHKAI GWGCWC 2 cut(s) 534, 590
BsiHKCI CYCGRG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 4 cut(s) 570, 870, 1148, 1213
BsmAI GTCTC 2 cut(s) 237, 1019
BsmFI GGGAC 1 cut(s) 151
BsnI GGCC 5 cut(s) 167, 269, 863, 950, 1003
Bso31I GGTCTC 1 cut(s) 237
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 534, 590
Bsp1407I TGTACA 1 cut(s) 807
BspACI CCGC 1 cut(s) 1170
BspANI GGCC 5 cut(s) 167, 269, 863, 950, 1003
BspCNI CTCAG 1 cut(s) 556
BspHI TCATGA 1 cut(s) 1381
BspLI GGNNCC 1 cut(s) 1244
BspMAI CTGCAG 1 cut(s) 1001
BspMI ACCTGC 3 cut(s) 193, 388, 799
BspT107I GGYRCC 1 cut(s) 1242
BspTI CTTAAG 2 cut(s) 452, 565
BspTNI GGTCTC 1 cut(s) 237
BsrDI GCAATG 2 cut(s) 420, 762
BsrGI TGTACA 1 cut(s) 807
BsrI ACTGG 5 cut(s) 270, 857, 874, 881, 1197
Bst4CI ACNGT 4 cut(s) 677, 781, 925, 1032
Bst6I CTCTTC 1 cut(s) 102
BstAFI CTTAAG 2 cut(s) 452, 565
BstAUI TGTACA 1 cut(s) 807
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 150, 1001
BstDEI CTNAG 2 cut(s) 543, 1067
BstF5I GGATG 4 cut(s) 319, 487, 695, 986
BstMAI GTCTC 2 cut(s) 237, 1019
BstMWI GCNNNNNNNGC 7 cut(s) 71, 185, 266, 594, 603, 719, 728
BstNSI RCATGY 1 cut(s) 329
BstPAI GACNNNNGTC 1 cut(s) 941
BstSFI CTRYAG 2 cut(s) 997, 1290
BstSLI GKGCMC 1 cut(s) 590
BstV2I GAAGAC 2 cut(s) 906, 1243
BsuRI GGCC 5 cut(s) 167, 269, 863, 950, 1003
BtsCI GGATG 4 cut(s) 319, 487, 695, 986
BtsI GCAGTG 1 cut(s) 1323
BtsIMutI CAGTG 7 cut(s) 263, 777, 867, 921, 1028, 1190, 1323
BveI ACCTGC 3 cut(s) 193, 388, 799
Cac8I GCNNGC 2 cut(s) 150, 1001
CciI TCATGA 1 cut(s) 1381
Csp6I GTAC 3 cut(s) 808, 874, 1095
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 9 cut(s) 128, 141, 161, 273, 326, 946, 1102, 1138, 1382
CviQI GTAC 3 cut(s) 808, 874, 1095
DdeI CTNAG 2 cut(s) 543, 1067
DrdI GACNNNNNNGTC 1 cut(s) 1033
DseDI GACNNNNNNGTC 1 cut(s) 1033
EaeI YGGCCR 3 cut(s) 165, 267, 948
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 532
Eco24I GRGCYC 1 cut(s) 534
Eco31I GGTCTC 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 532
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 534
FaeI CATG 9 cut(s) 131, 144, 164, 276, 329, 949, 1105, 1141, 1385
FaqI GGGAC 1 cut(s) 151
FatI CATG 9 cut(s) 127, 140, 160, 272, 325, 945, 1101, 1137, 1381
FauNDI CATATG 1 cut(s) 1009
FokI GGATG 4 cut(s) 326, 494, 682, 993
FriOI GRGCYC 1 cut(s) 534
FspBI CTAG 4 cut(s) 282, 1079, 1124, 1230
GsaI CCCAGC 1 cut(s) 444
HaeIII GGCC 5 cut(s) 167, 269, 863, 950, 1003
Hin1II CATG 9 cut(s) 131, 144, 164, 276, 329, 949, 1105, 1141, 1385
HinfI GANTC 2 cut(s) 59, 119
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 4 cut(s) 463, 588, 808, 1185
Hpy188I TCNGA 2 cut(s) 728, 990
Hpy188III TCNNGA 4 cut(s) 764, 978, 1363, 1382
Hpy8I GTNNAC 4 cut(s) 463, 588, 808, 1185
HpyAV CCTTC 2 cut(s) 724, 874
HpyCH4III ACNGT 4 cut(s) 677, 781, 925, 1032
HpyCH4IV ACGT 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 7 cut(s) 71, 185, 266, 594, 603, 719, 728
HpyF3I CTNAG 2 cut(s) 543, 1067
HpySE526I ACGT 1 cut(s) 12
Hsp92II CATG 9 cut(s) 131, 144, 164, 276, 329, 949, 1105, 1141, 1385
LmnI GCTCC 3 cut(s) 552, 728, 1172
LweI GCATC 2 cut(s) 742, 971
MaeI CTAG 4 cut(s) 282, 1079, 1124, 1230
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 6 cut(s) 328, 485, 628, 671, 848, 888
MboII GAAGA 9 cut(s) 92, 110, 119, 128, 331, 906, 1245, 1248, 1367
MfeI CAATTG 3 cut(s) 732, 857, 1176
MhlI GDGCHC 2 cut(s) 534, 590
MlsI TGGCCA 3 cut(s) 167, 269, 950
MluCI AATT 5 cut(s) 432, 633, 732, 857, 1176
MluNI TGGCCA 3 cut(s) 167, 269, 950
MnlI CCTC 7 cut(s) 70, 103, 210, 939, 1015, 1072, 1108
Mox20I TGGCCA 3 cut(s) 167, 269, 950
MscI TGGCCA 3 cut(s) 167, 269, 950
MseI TTAA 5 cut(s) 26, 453, 491, 566, 1281
MslI CAYNNNNRTG 3 cut(s) 480, 1106, 1256
Msp20I TGGCCA 3 cut(s) 167, 269, 950
MspA1I CMGCKG 1 cut(s) 440
MspCI CTTAAG 2 cut(s) 452, 565
MunI CAATTG 3 cut(s) 732, 857, 1176
MwoI GCNNNNNNNGC 7 cut(s) 71, 185, 266, 594, 603, 719, 728
NdeI CATATG 1 cut(s) 1009
NlaIII CATG 9 cut(s) 131, 144, 164, 276, 329, 949, 1105, 1141, 1385
NlaIV GGNNCC 1 cut(s) 1244
NmeAIII GCCGAG 1 cut(s) 1286
NmuCI GTSAC 3 cut(s) 485, 628, 888
NspI RCATGY 1 cut(s) 329
PagI TCATGA 1 cut(s) 1381
PaqCI CACCTGC 1 cut(s) 193
PciI ACATGT 1 cut(s) 325
PfeI GAWTC 2 cut(s) 59, 119
PflMI CCANNNNNTGG 1 cut(s) 1148
Ppu21I YACGTR 1 cut(s) 13
PscI ACATGT 1 cut(s) 325
PshAI GACNNNNGTC 1 cut(s) 941
PsiI TTATAA 2 cut(s) 639, 1371
Psp124BI GAGCTC 1 cut(s) 534
PspFI CCCAGC 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 1244
PstI CTGCAG 1 cut(s) 1001
PvuII CAGCTG 1 cut(s) 440
RsaI GTAC 3 cut(s) 809, 875, 1096
RsaNI GTAC 3 cut(s) 808, 874, 1095
RseI CAYNNNNRTG 3 cut(s) 480, 1106, 1256
SacI GAGCTC 1 cut(s) 534
SaqAI TTAA 5 cut(s) 26, 453, 491, 566, 1281
SduI GDGCHC 2 cut(s) 534, 590
SfaNI GCATC 2 cut(s) 742, 971
SfcI CTRYAG 2 cut(s) 997, 1290
SmiMI CAYNNNNRTG 3 cut(s) 480, 1106, 1256
SmlI CTYRAG 2 cut(s) 452, 565
SmoI CTYRAG 2 cut(s) 452, 565
SpeI ACTAGT 1 cut(s) 1123
Sse9I AATT 5 cut(s) 432, 633, 732, 857, 1176
SsiI CCGC 1 cut(s) 1170
SspMI CTAG 4 cut(s) 282, 1079, 1124, 1230
SstI GAGCTC 1 cut(s) 534
TaaI ACNGT 4 cut(s) 677, 781, 925, 1032
TaiI ACGT 1 cut(s) 15
TaqI TCGA 1 cut(s) 1020
TasI AATT 5 cut(s) 432, 633, 732, 857, 1176
TatI WGTACW 1 cut(s) 807
TfiI GAWTC 2 cut(s) 59, 119
Tru1I TTAA 5 cut(s) 26, 453, 491, 566, 1281
Tru9I TTAA 5 cut(s) 26, 453, 491, 566, 1281
TscAI CASTG 7 cut(s) 270, 784, 874, 928, 1035, 1197, 1323
TseFI GTSAC 3 cut(s) 485, 628, 888
Tsp45I GTSAC 3 cut(s) 485, 628, 888
TspDTI ATGAA 4 cut(s) 17, 149, 1056, 1370
TspRI CASTG 7 cut(s) 270, 784, 874, 928, 1035, 1197, 1323
Van91I CCANNNNNTGG 1 cut(s) 1148
Vha464I CTTAAG 2 cut(s) 452, 565
VneI GTGCAC 1 cut(s) 586
XceI RCATGY 1 cut(s) 329
XspI CTAG 4 cut(s) 282, 1079, 1124, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.