Rroxscaffold_7G00179340

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0017246
Physical Location & Seq
Reverse (-)
18949490 .. 18951897
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179340.1

Sequence Viewer

Length: 1395 bp
ATGACACAACCACCAGATTCAACAGAAGCAGCAGAGGAAGAAAGGCAGCGAGTAGAAGAAGCAGAAGAAGCAGAAGCAAAACATGGCAAAGGGACATGGAAGCACGCAGCGTTTCATGTGGCCACAACGATTGCCACACCTGCTGCCTATGCTCCTCTGCCTTTTGCTCTCGCTTCTCTTGGTTGGCCTCTCGGGGTAAGCAGCTTGGTAGGTGCAACACTGGCCACATGGTATTCTAGTCTGCTCATCGCTTCTCTGTGGAAGTGGGATGGGAAGAAACATGTTACGTATCGCCACCTTGCCCAAAGCATTTTCGGTACACTTTCAACAACTTATCTATCTGCCACTCTAATCAGCGCGCATCGGTTCTGGGGTTATTGGTCTATTGCTTTTTTTCAGCAGGTAGCTTCAATAGGCAATAACATTGCCATTCAAATTGCAGCTGGGAGTAGCCTTAAGGCTGTTTACAAGTATTATCACAAGGATGGTGACTTAACTCTGCAACATTTTGTTATTTTCTTTGGGGTCTTTGAGCTCTTTCTCTCTCAGCTCCCTGATATACACTCCTTAAGATGGGTAAATGCCTTGTGCACTTTCAGCACAGTTGGCTTTGCTGCTACAACCATTGGTGTCACAATTTATAATGGGAAAAAGATTGATAGAGAATCAATCAGTTACGGTTTAGATGGAAGTGCATCATCCAAAGTCTTTAGAGCCTTCAATGCTCTCGGAGCAATTGCCTTTTCCTTTGGAGATGCAATGCTTCCAGAGATACAGAACACTGTGAGAGAACCTGCAAAGAAAAATGTGTACAAAGGTGTATCAGCAGCTTATGGTATCATAGTGTTGAGTTACTGGCAATTGGCCTTCACTGGGTACTGGGCTTTTGGGTCACAAGTTGAGCCTTACCTCTTGTCTTCACTTACGGTTCCTCAATGGACTATTGTCATGGCCAATCTTTTTGCTGTCATACAAATATCAGGATGCTTTCAGATTTACTGCAGGCCAACATATGCCTACTTCGAGGAGAGACTGTTGTCTAATGAAACTGCTACAAGTCGTTCCTCACTAAGAAACAAGCTAGTTCGTCTTGTTTGTACCTCCATGTATATGCTACTTATTACACTAGTTGCAGCAGCCATGCCATTCTTTGGGGATTTTGTGGCAATATGCGGAGCAATTGGGTTCACACCACTGGACTTTGTGTTCCCTGCTATGGCATATCTAAAAGCTAGAAGAAGACGGCACCAAAACACCAAAATGTATCTGTCTTTGCTACTTCTTAACTTTGCTATAGTGGTTTGGTTCTCGGCTGTTGCAGTGTTCGGTTGCATTGGGGCAGTTAAGTTTATTGTGGAAGATGTCAAGACTTATAAGTTCTTTCATGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

464

Amino Acids

51.59

Weight (kDa)

9.01

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 30 - 446 3.2e-67 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22320
malus_domestica MD10G1191600.v1.1
prunus_persica Prupe.8G230900_v2.0.a1
pyrus_communis pycom10g16520
rosa_chinensis RchiOBHm_Chr6g0289321
rosa_laevigata RLG00000012313
rosa_multiflora Rmu_sc0001527.1_g000025 Rmu_sc0005115.1_g000002
rosa_roxburghii Rroxscaffold_7G00179340
rosa_rugosa Rorug06G0202800 Rorug06G0203000
rosa_samantha Rh6AG313600
rosa_wichuraiana Rw6G027110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 642, 1374
AarI CACCTGC 1 cut(s) 148
Acc36I ACCTGC 3 cut(s) 148, 391, 802
AccB1I GGYRCC 1 cut(s) 1245
AccB7I CCANNNNNTGG 1 cut(s) 1151
AccII CGCG 1 cut(s) 359
AciI CCGC 1 cut(s) 1173
AcoI YGGCCR 3 cut(s) 120, 222, 951
AfaI GTAC 4 cut(s) 319, 812, 878, 1099
AfiI CCNNNNNNNGG 4 cut(s) 573, 873, 1151, 1216
AflII CTTAAG 2 cut(s) 455, 568
AflIII ACRYGT 1 cut(s) 280
AgsI TTSAA 5 cut(s) 21, 327, 411, 434, 721
AhlI ACTAGT 1 cut(s) 1126
AloI GAACNNNNNNTCC 2 cut(s) 1190, 1222
AluBI AGCT 8 cut(s) 204, 407, 443, 535, 550, 830, 1081, 1232
AluI AGCT 8 cut(s) 204, 407, 443, 535, 550, 830, 1081, 1232
Alw21I GWGCWC 2 cut(s) 537, 593
Alw26I GTCTC 1 cut(s) 1024
Alw44I GTGCAC 1 cut(s) 589
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 6 cut(s) 120, 185, 222, 864, 951, 1004
ApaLI GTGCAC 1 cut(s) 589
AspLEI GCGC 2 cut(s) 359, 361
AsuHPI GGTGA 1 cut(s) 500
AvaI CYCGRG 1 cut(s) 191
BaeGI GKGCMC 1 cut(s) 593
BalI TGGCCA 3 cut(s) 122, 224, 953
BanI GGYRCC 1 cut(s) 1245
BanII GRGCYC 1 cut(s) 537
BbsI GAAGAC 2 cut(s) 909, 1246
Bbv12I GWGCWC 2 cut(s) 537, 593
BccI CCATC 4 cut(s) 263, 479, 567, 680
BceAI ACGGC 1 cut(s) 1259
BcoDI GTCTC 1 cut(s) 1024
BcuI ACTAGT 1 cut(s) 1126
BfaI CTAG 4 cut(s) 237, 1082, 1127, 1233
BfmI CTRYAG 2 cut(s) 1000, 1293
BfrI CTTAAG 2 cut(s) 455, 568
BfuAI ACCTGC 3 cut(s) 148, 391, 802
BmeT110I CYCGRG 1 cut(s) 191
BmiI GGNNCC 2 cut(s) 930, 1247
BmrI ACTGGG 2 cut(s) 882, 889
BmsI GCATC 4 cut(s) 370, 704, 745, 974
BmuI ACTGGG 2 cut(s) 882, 889
BoxI GACNNNNGTC 2 cut(s) 944, 1036
BpiI GAAGAC 2 cut(s) 909, 1246
BsaAI YACGTR 1 cut(s) 288
BsaXI ACNNNNNCTCC 2 cut(s) 1019, 1049
Bsc4I CCNNNNNNNGG 4 cut(s) 573, 873, 1151, 1216
Bse1I ACTGG 5 cut(s) 225, 860, 877, 884, 1200
Bse3DI GCAATG 2 cut(s) 423, 765
BseGI GGATG 4 cut(s) 274, 490, 698, 989
BseLI CCNNNNNNNGG 4 cut(s) 573, 873, 1151, 1216
BseMI GCAATG 2 cut(s) 423, 765
BseMII CTCAG 1 cut(s) 560
BseNI ACTGG 5 cut(s) 225, 860, 877, 884, 1200
BsePI GCGCGC 1 cut(s) 357
BseRI GAGGAG 2 cut(s) 144, 1040
BseSI GKGCMC 1 cut(s) 593
BseYI CCCAGC 1 cut(s) 443
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 6 cut(s) 122, 187, 224, 866, 953, 1006
BshNI GGYRCC 1 cut(s) 1245
BsiHKAI GWGCWC 2 cut(s) 537, 593
BsiHKCI CYCGRG 1 cut(s) 191
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 4 cut(s) 573, 873, 1151, 1216
BsmAI GTCTC 1 cut(s) 1024
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 6 cut(s) 122, 187, 224, 866, 953, 1006
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 2 cut(s) 537, 593
Bsp1407I TGTACA 1 cut(s) 810
BspACI CCGC 1 cut(s) 1173
BspANI GGCC 6 cut(s) 122, 187, 224, 866, 953, 1006
BspCNI CTCAG 1 cut(s) 559
BspFNI CGCG 1 cut(s) 359
BspHI TCATGA 1 cut(s) 1384
BspLI GGNNCC 2 cut(s) 930, 1247
BspMAI CTGCAG 1 cut(s) 1004
BspMI ACCTGC 3 cut(s) 148, 391, 802
BspT107I GGYRCC 1 cut(s) 1245
BspTI CTTAAG 2 cut(s) 455, 568
BsrDI GCAATG 2 cut(s) 423, 765
BsrGI TGTACA 1 cut(s) 810
BsrI ACTGG 5 cut(s) 225, 860, 877, 884, 1200
BssHII GCGCGC 1 cut(s) 357
Bst4CI ACNGT 5 cut(s) 604, 680, 784, 928, 1035
BstAFI CTTAAG 2 cut(s) 455, 568
BstAUI TGTACA 1 cut(s) 810
BstBAI YACGTR 1 cut(s) 288
BstC8I GCNNGC 3 cut(s) 105, 359, 1004
BstDEI CTNAG 2 cut(s) 546, 1070
BstF5I GGATG 4 cut(s) 274, 490, 698, 989
BstFNI CGCG 1 cut(s) 359
BstHHI GCGC 2 cut(s) 359, 361
BstMAI GTCTC 1 cut(s) 1024
BstMWI GCNNNNNNNGC 8 cut(s) 68, 140, 149, 221, 597, 606, 722, 731
BstNSI RCATGY 1 cut(s) 284
BstPAI GACNNNNGTC 2 cut(s) 944, 1036
BstSFI CTRYAG 2 cut(s) 1000, 1293
BstSLI GKGCMC 1 cut(s) 593
BstSNI TACGTA 1 cut(s) 288
BstUI CGCG 1 cut(s) 359
BstV2I GAAGAC 2 cut(s) 909, 1246
BsuRI GGCC 6 cut(s) 122, 187, 224, 866, 953, 1006
BtgZI GCGATG 1 cut(s) 232
BtsCI GGATG 4 cut(s) 274, 490, 698, 989
BtsI GCAGTG 1 cut(s) 1326
BtsIMutI CAGTG 5 cut(s) 218, 780, 870, 1193, 1326
BveI ACCTGC 3 cut(s) 148, 391, 802
Cac8I GCNNGC 3 cut(s) 105, 359, 1004
CciI TCATGA 1 cut(s) 1384
CfoI GCGC 2 cut(s) 359, 361
Csp6I GTAC 4 cut(s) 318, 811, 877, 1098
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 9 cut(s) 83, 96, 116, 228, 281, 949, 1105, 1141, 1385
CviQI GTAC 4 cut(s) 318, 811, 877, 1098
DdeI CTNAG 2 cut(s) 546, 1070
EaeI YGGCCR 3 cut(s) 120, 222, 951
Ecl136II GAGCTC 1 cut(s) 535
Eco105I TACGTA 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 535
EcoT38I GRGCYC 1 cut(s) 537
FaeI CATG 9 cut(s) 86, 99, 119, 231, 284, 952, 1108, 1144, 1388
FaqI GGGAC 1 cut(s) 106
FatI CATG 9 cut(s) 82, 95, 115, 227, 280, 948, 1104, 1140, 1384
FauNDI CATATG 1 cut(s) 1012
FokI GGATG 4 cut(s) 281, 497, 685, 996
FriOI GRGCYC 1 cut(s) 537
FspBI CTAG 4 cut(s) 237, 1082, 1127, 1233
GlaI GCGC 2 cut(s) 358, 360
GsaI CCCAGC 1 cut(s) 447
HaeIII GGCC 6 cut(s) 122, 187, 224, 866, 953, 1006
HhaI GCGC 2 cut(s) 359, 361
Hin1II CATG 9 cut(s) 86, 99, 119, 231, 284, 952, 1108, 1144, 1388
Hin6I GCGC 2 cut(s) 357, 359
HinP1I GCGC 2 cut(s) 357, 359
HinfI GANTC 2 cut(s) 17, 665
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 5 cut(s) 320, 466, 591, 811, 1188
Hpy188I TCNGA 2 cut(s) 731, 993
Hpy188III TCNNGA 4 cut(s) 767, 981, 1366, 1385
Hpy8I GTNNAC 5 cut(s) 320, 466, 591, 811, 1188
HpyAV CCTTC 2 cut(s) 727, 877
HpyCH4III ACNGT 5 cut(s) 604, 680, 784, 928, 1035
HpyCH4IV ACGT 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 140, 149, 221, 597, 606, 722, 731
HpyF3I CTNAG 2 cut(s) 546, 1070
HpySE526I ACGT 1 cut(s) 287
Hsp92II CATG 9 cut(s) 86, 99, 119, 231, 284, 952, 1108, 1144, 1388
HspAI GCGC 2 cut(s) 357, 359
LmnI GCTCC 4 cut(s) 157, 555, 731, 1175
LweI GCATC 4 cut(s) 370, 704, 745, 974
MaeI CTAG 4 cut(s) 237, 1082, 1127, 1233
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 6 cut(s) 283, 488, 631, 674, 851, 891
MboII GAAGA 8 cut(s) 50, 68, 77, 286, 909, 1248, 1251, 1370
MfeI CAATTG 3 cut(s) 735, 860, 1179
MhlI GDGCHC 2 cut(s) 537, 593
MlsI TGGCCA 3 cut(s) 122, 224, 953
MluCI AATT 5 cut(s) 435, 636, 735, 860, 1179
MluNI TGGCCA 3 cut(s) 122, 224, 953
MnlI CCTC 8 cut(s) 28, 165, 198, 920, 942, 1018, 1075, 1111
Mox20I TGGCCA 3 cut(s) 122, 224, 953
MscI TGGCCA 3 cut(s) 122, 224, 953
MseI TTAA 5 cut(s) 456, 494, 569, 1284, 1344
MslI CAYNNNNRTG 3 cut(s) 483, 1109, 1259
Msp20I TGGCCA 3 cut(s) 122, 224, 953
MspA1I CMGCKG 1 cut(s) 443
MspCI CTTAAG 2 cut(s) 455, 568
MunI CAATTG 3 cut(s) 735, 860, 1179
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 8 cut(s) 68, 140, 149, 221, 597, 606, 722, 731
NdeI CATATG 1 cut(s) 1012
NlaIII CATG 9 cut(s) 86, 99, 119, 231, 284, 952, 1108, 1144, 1388
NlaIV GGNNCC 2 cut(s) 930, 1247
NmeAIII GCCGAG 1 cut(s) 1289
NmuCI GTSAC 3 cut(s) 488, 631, 891
NspI RCATGY 1 cut(s) 284
PagI TCATGA 1 cut(s) 1384
PaqCI CACCTGC 1 cut(s) 148
PauI GCGCGC 1 cut(s) 357
PciI ACATGT 1 cut(s) 280
PfeI GAWTC 2 cut(s) 17, 665
PflMI CCANNNNNTGG 1 cut(s) 1151
Ppu21I YACGTR 1 cut(s) 288
PscI ACATGT 1 cut(s) 280
PshAI GACNNNNGTC 2 cut(s) 944, 1036
PsiI TTATAA 2 cut(s) 642, 1374
Psp124BI GAGCTC 1 cut(s) 537
PspFI CCCAGC 1 cut(s) 443
PspN4I GGNNCC 2 cut(s) 930, 1247
PstI CTGCAG 1 cut(s) 1004
PteI GCGCGC 1 cut(s) 357
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 4 cut(s) 319, 812, 878, 1099
RsaNI GTAC 4 cut(s) 318, 811, 877, 1098
RseI CAYNNNNRTG 3 cut(s) 483, 1109, 1259
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 5 cut(s) 456, 494, 569, 1284, 1344
SduI GDGCHC 2 cut(s) 537, 593
SfaNI GCATC 4 cut(s) 370, 704, 745, 974
SfcI CTRYAG 2 cut(s) 1000, 1293
SmiMI CAYNNNNRTG 3 cut(s) 483, 1109, 1259
SmlI CTYRAG 2 cut(s) 455, 568
SmoI CTYRAG 2 cut(s) 455, 568
SnaBI TACGTA 1 cut(s) 288
SpeI ACTAGT 1 cut(s) 1126
Sse9I AATT 5 cut(s) 435, 636, 735, 860, 1179
SsiI CCGC 1 cut(s) 1173
SspMI CTAG 4 cut(s) 237, 1082, 1127, 1233
SstI GAGCTC 1 cut(s) 537
TaaI ACNGT 5 cut(s) 604, 680, 784, 928, 1035
TaiI ACGT 1 cut(s) 290
TaqI TCGA 1 cut(s) 1023
TasI AATT 5 cut(s) 435, 636, 735, 860, 1179
TatI WGTACW 1 cut(s) 810
TfiI GAWTC 2 cut(s) 17, 665
Tru1I TTAA 5 cut(s) 456, 494, 569, 1284, 1344
Tru9I TTAA 5 cut(s) 456, 494, 569, 1284, 1344
TscAI CASTG 5 cut(s) 225, 787, 877, 1200, 1326
TseFI GTSAC 3 cut(s) 488, 631, 891
Tsp45I GTSAC 3 cut(s) 488, 631, 891
TspDTI ATGAA 3 cut(s) 104, 1059, 1373
TspRI CASTG 5 cut(s) 225, 787, 877, 1200, 1326
Van91I CCANNNNNTGG 1 cut(s) 1151
Vha464I CTTAAG 2 cut(s) 455, 568
VneI GTGCAC 1 cut(s) 589
XceI RCATGY 1 cut(s) 284
XspI CTAG 4 cut(s) 237, 1082, 1127, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.