Prupe.8G230900_v2.0.a1

GABA transporter 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0017246
Physical Location & Seq
Forward (+)
20587515 .. 20589711
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G230900.1

Sequence Viewer

Length: 1350 bp
ATGTCACAAACAGAACTACCAAATTCAACAGCAGAAGTGGAAGTAGAAAGACCAGTAGTAGTACTAGTAGTAGAAGAAGAAGCAGCAAAACAAGGCAAAGGGACGTGGAAGCACGCTGCTTTCCACATGGCCACCACGATTGCCACACCGGCCGCTTATGCTCCCCTGCCTTTTGCTCTCGCTTCTCTCGGTTGGCCTCTCGGGGTAAGCAGTTTGGTGGCTGCAACACTAGCCACGTGGTATTCTAGCTTTCTCATTGCATCTCTGTGGAATTGGGATGGGAAGAAACATGTCACCTATCGCCACTTAGCACACAGCATTTTTGGGTTCTGGGGTTATTGGTCAATTGCATTTTTTCAACAGGTAGCCTCTGTAGGCAATAATATTGCCATTCATATTGCAGCTGGAAACAGCCTTAAGGCTGTTTACAAATATTATGACAAGGATGGTGGCTTAACCCTGCAGCATTTTATTATTTTCTTTGGGGCCTTTGAGCTCTTGCTGTCTCAGCTCCCTGATATTCACTCCTTGAGATGGGTAAATGCCTTGTGCACTTTCAGCACCATTGGCTTTGCTGGCACAACCATTGGTGTCACTATTTATAACGGGAAAAAGATTGATAGGAAATCAGTCAGTTACAGATTGGATGGAAGCTCATCTTCTAAAGCCTTCAGAGCCTTTAATGCTCTTGGGGCAATTGCCTTTTCCTTTGGAGATGCCATGCTTCCAGAGATACAGAATACTGTGAGAGAACCTGCAAAGAAGAACATGTATAAAGGAGTATCAGCAGCATATGCTGTCATTGTGTTGAGCTACTGGCAATTGGCCTTCACTGGCTACTGGGCTTTTGGTTCACAAGTCCAGCCTTACATCTTGTCTTCCCTAACTGTTCCCCAATGGACTATTGTCATGGCCAATCTTTTTGCTGTTATACAGATCTCAGGATGTTTTCAGATATATTGCAGGCCAACATATGCCTACTTTGAGGAAAGACTATTGTCTCACAAAACTACCAATCACTTCACATCCAGAAACAATCTAATTCGTCTTGTTTTTACGTCCATGTATATGGTTCTCATTACTCTTGTTGCAGCAGCCATGCCTTTCTTTGGGGATTTTGTGTCAATCTGTGGAGCAGTTGGGTTCACTCCACTGGATTTTGTGTTCCCTGCTCTGGCATATCTAAAAGCTAGAAGACGGCCCCAAAATACCAAAATTTACCTTTCTTTGCTGCTTCTTAACTTTGCCATGGTGGCTTGGTTCTCCAGTGTTGCAGTGTTGGGTTGCATTGGTGCAGTCAAGTTCATTGTGGAAGATGTCAAGACTTACAAATTCTTTCATGATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

450

Amino Acids

49.94

Weight (kDa)

9.28

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017246)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22320
malus_domestica MD10G1191600.v1.1
prunus_persica Prupe.8G230900_v2.0.a1
pyrus_communis pycom10g16520
rosa_chinensis RchiOBHm_Chr6g0289321
rosa_laevigata RLG00000012313
rosa_multiflora Rmu_sc0001527.1_g000025 Rmu_sc0005115.1_g000002
rosa_roxburghii Rroxscaffold_7G00179340
rosa_rugosa Rorug06G0202800 Rorug06G0203000
rosa_samantha Rh6AG313600
rosa_wichuraiana Rw6G027110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
Acc36I ACCTGC 1 cut(s) 763
AciI CCGC 1 cut(s) 153
AcoI YGGCCR 3 cut(s) 129, 150, 912
AcsI RAATTY 3 cut(s) 22, 1215, 1331
AcuI CTGAAG 1 cut(s) 655
AcvI CACGTG 1 cut(s) 237
AfaI GTAC 1 cut(s) 63
AfiI CCNNNNNNNGG 3 cut(s) 534, 1109, 1174
AflII CTTAAG 1 cut(s) 416
AflIII ACRYGT 2 cut(s) 289, 768
AgsI TTSAA 2 cut(s) 27, 359
AhlI ACTAGT 1 cut(s) 64
AjiI CACGTC 1 cut(s) 105
AloI GAACNNNNNNTCC 2 cut(s) 1148, 1180
AluBI AGCT 7 cut(s) 249, 404, 496, 511, 654, 813, 1190
AluI AGCT 7 cut(s) 249, 404, 496, 511, 654, 813, 1190
Alw21I GWGCWC 2 cut(s) 498, 554
Alw26I GTCTC 2 cut(s) 510, 1005
Alw44I GTGCAC 1 cut(s) 550
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 8 cut(s) 129, 150, 194, 486, 825, 912, 965, 1199
ApaLI GTGCAC 1 cut(s) 550
ApeKI GCWGC 9 cut(s) 83, 116, 221, 401, 463, 788, 1091, 1094, 1231
ApoI RAATTY 3 cut(s) 22, 1215, 1331
AspS9I GGNCC 2 cut(s) 486, 1200
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 554
BalI TGGCCA 2 cut(s) 131, 914
BanII GRGCYC 1 cut(s) 498
BbrPI CACGTG 1 cut(s) 237
BbsI GAAGAC 2 cut(s) 870, 1201
Bbv12I GWGCWC 2 cut(s) 498, 554
BbvI GCAGC 9 cut(s) 95, 103, 208, 413, 475, 800, 1103, 1106, 1218
BccI CCATC 4 cut(s) 272, 440, 528, 641
BceAI ACGGC 1 cut(s) 1214
BcoDI GTCTC 2 cut(s) 510, 1005
BcuI ACTAGT 1 cut(s) 64
BfaI CTAG 4 cut(s) 65, 230, 246, 1191
BfmI CTRYAG 2 cut(s) 372, 461
BfrI CTTAAG 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 763
BglI GCCNNNNNGGC 2 cut(s) 149, 1253
BglII AGATCT 1 cut(s) 936
BmcAI AGTACT 1 cut(s) 63
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 486, 1200
BmiI GGNNCC 2 cut(s) 487, 1202
BmrI ACTGGG 1 cut(s) 850
BmsI GCATC 2 cut(s) 269, 706
BmuI ACTGGG 1 cut(s) 850
BoxI GACNNNNGTC 2 cut(s) 905, 997
BpiI GAAGAC 2 cut(s) 870, 1201
BpmI CTGGAG 1 cut(s) 1249
BpuEI CTTGAG 1 cut(s) 550
BsaAI YACGTR 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 1248
Bsc4I CCNNNNNNNGG 3 cut(s) 534, 1109, 1174
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 6 cut(s) 53, 821, 838, 845, 1158, 1266
Bse3DI GCAATG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 1248
BseGI GGATG 5 cut(s) 283, 451, 652, 950, 1025
BseLI CCNNNNNNNGG 3 cut(s) 534, 1109, 1174
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 2 cut(s) 521, 954
BseNI ACTGG 6 cut(s) 53, 821, 838, 845, 1158, 1266
BseSI GKGCMC 1 cut(s) 554
BseX3I CGGCCG 1 cut(s) 150
BseXI GCAGC 9 cut(s) 95, 103, 208, 413, 475, 800, 1103, 1106, 1218
BsgI GTGCAG 1 cut(s) 1314
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 8 cut(s) 131, 152, 196, 488, 827, 914, 967, 1201
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 2 cut(s) 498, 554
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 3 cut(s) 534, 1109, 1174
BsmAI GTCTC 2 cut(s) 510, 1005
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 8 cut(s) 131, 152, 196, 488, 827, 914, 967, 1201
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 498, 554
Bsp143I GATC 1 cut(s) 936
Bsp19I CCATGG 1 cut(s) 1248
BspACI CCGC 1 cut(s) 153
BspANI GGCC 8 cut(s) 131, 152, 196, 488, 827, 914, 967, 1201
BspCNI CTCAG 2 cut(s) 520, 953
BspHI TCATGA 1 cut(s) 1339
BspLI GGNNCC 2 cut(s) 487, 1202
BspMAI CTGCAG 1 cut(s) 465
BspMI ACCTGC 1 cut(s) 763
BspTI CTTAAG 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 255
BsrFI RCCGGY 1 cut(s) 148
BsrI ACTGG 6 cut(s) 53, 821, 838, 845, 1158, 1266
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 1 cut(s) 1248
BssMI GATC 1 cut(s) 936
BssT1I CCWWGG 1 cut(s) 1248
Bst4CI ACNGT 2 cut(s) 745, 889
BstAFI CTTAAG 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 114, 577, 965
BstDEI CTNAG 3 cut(s) 307, 507, 940
BstDSI CCRYGG 1 cut(s) 1248
BstF5I GGATG 5 cut(s) 283, 451, 652, 950, 1025
BstKTI GATC 1 cut(s) 939
BstMAI GTCTC 2 cut(s) 510, 1005
BstMBI GATC 1 cut(s) 936
BstMCI CGRYCG 1 cut(s) 153
BstNSI RCATGY 2 cut(s) 293, 772
BstPAI GACNNNNGTC 2 cut(s) 905, 997
BstSFI CTRYAG 2 cut(s) 372, 461
BstSLI GKGCMC 1 cut(s) 554
BstV1I GCAGC 9 cut(s) 95, 103, 208, 413, 475, 800, 1103, 1106, 1218
BstV2I GAAGAC 2 cut(s) 870, 1201
BstX2I RGATCY 1 cut(s) 936
BstXI CCANNNNNNTGG 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 936
BstZI CGGCCG 1 cut(s) 150
BsuRI GGCC 8 cut(s) 131, 152, 196, 488, 827, 914, 967, 1201
BtgI CCRYGG 1 cut(s) 1248
BtrI CACGTC 1 cut(s) 105
BtsCI GGATG 5 cut(s) 283, 451, 652, 950, 1025
BtsI GCAGTG 1 cut(s) 1281
BtsIMutI CAGTG 4 cut(s) 831, 1151, 1273, 1281
BveI ACCTGC 1 cut(s) 763
Cac8I GCNNGC 3 cut(s) 114, 577, 965
CciI TCATGA 1 cut(s) 1339
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 486, 1200
Csp6I GTAC 1 cut(s) 62
CspCI CAANNNNNGTGG 4 cut(s) 121, 156, 430, 465
CviAII CATG 9 cut(s) 127, 290, 721, 769, 910, 1063, 1099, 1249, 1340
CviQI GTAC 1 cut(s) 62
DdeI CTNAG 3 cut(s) 307, 507, 940
DpnI GATC 1 cut(s) 938
DpnII GATC 1 cut(s) 936
EaeI YGGCCR 3 cut(s) 129, 150, 912
EagI CGGCCG 1 cut(s) 150
Ecl136II GAGCTC 1 cut(s) 496
EclXI CGGCCG 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 1248
Eco24I GRGCYC 1 cut(s) 498
Eco52I CGGCCG 1 cut(s) 150
Eco53kI GAGCTC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 655
Eco72I CACGTG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 496
EcoO109I RGGNCCY 1 cut(s) 486
EcoT14I CCWWGG 1 cut(s) 1248
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 1248
FaeI CATG 9 cut(s) 130, 293, 724, 772, 913, 1066, 1102, 1252, 1343
FalI AAGNNNNNCTT 2 cut(s) 643, 675
FaqI GGGAC 1 cut(s) 115
FatI CATG 9 cut(s) 126, 289, 720, 768, 909, 1062, 1098, 1248, 1339
FauNDI CATATG 2 cut(s) 793, 973
FokI GGATG 5 cut(s) 290, 458, 659, 957, 1012
FriOI GRGCYC 1 cut(s) 498
FspBI CTAG 4 cut(s) 65, 230, 246, 1191
GsuI CTGGAG 1 cut(s) 1249
HaeIII GGCC 8 cut(s) 131, 152, 196, 488, 827, 914, 967, 1201
HapII CCGG 1 cut(s) 149
Hin1II CATG 9 cut(s) 130, 293, 724, 772, 913, 1066, 1102, 1252, 1343
HpaII CCGG 1 cut(s) 149
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 4 cut(s) 427, 552, 854, 1146
Hpy188I TCNGA 2 cut(s) 674, 954
Hpy188III TCNNGA 5 cut(s) 728, 942, 1029, 1321, 1340
Hpy8I GTNNAC 4 cut(s) 427, 552, 854, 1146
HpyAV CCTTC 2 cut(s) 679, 838
HpyCH4III ACNGT 2 cut(s) 745, 889
HpyCH4IV ACGT 3 cut(s) 104, 236, 1058
HpyF3I CTNAG 3 cut(s) 307, 507, 940
HpySE526I ACGT 3 cut(s) 104, 236, 1058
Hsp92II CATG 9 cut(s) 130, 293, 724, 772, 913, 1066, 1102, 1252, 1343
Kzo9I GATC 1 cut(s) 936
LmnI GCTCC 3 cut(s) 166, 516, 1133
Lsp1109I GCAGC 9 cut(s) 95, 103, 208, 413, 475, 800, 1103, 1106, 1218
LweI GCATC 2 cut(s) 269, 706
MaeI CTAG 4 cut(s) 65, 230, 246, 1191
MaeII ACGT 3 cut(s) 104, 236, 1058
MaeIII GTNAC 4 cut(s) 3, 292, 592, 635
MalI GATC 1 cut(s) 938
MboI GATC 1 cut(s) 936
MboII GAAGA 8 cut(s) 86, 89, 295, 651, 775, 870, 1206, 1325
MfeI CAATTG 3 cut(s) 345, 696, 821
MflI RGATCY 1 cut(s) 936
MhlI GDGCHC 2 cut(s) 498, 554
MlsI TGGCCA 2 cut(s) 131, 914
MluCI AATT 8 cut(s) 22, 271, 345, 696, 821, 1041, 1215, 1331
MluNI TGGCCA 2 cut(s) 131, 914
MnlI CCTC 3 cut(s) 207, 379, 979
Mox20I TGGCCA 2 cut(s) 131, 914
MscI TGGCCA 2 cut(s) 131, 914
MseI TTAA 4 cut(s) 417, 455, 681, 1239
MslI CAYNNNNRTG 2 cut(s) 265, 1067
Msp20I TGGCCA 2 cut(s) 131, 914
MspA1I CMGCKG 1 cut(s) 404
MspCI CTTAAG 1 cut(s) 416
MspI CCGG 1 cut(s) 149
MunI CAATTG 3 cut(s) 345, 696, 821
NcoI CCATGG 1 cut(s) 1248
NdeI CATATG 2 cut(s) 793, 973
NdeII GATC 1 cut(s) 936
NlaIII CATG 9 cut(s) 130, 293, 724, 772, 913, 1066, 1102, 1252, 1343
NlaIV GGNNCC 2 cut(s) 487, 1202
NmuCI GTSAC 3 cut(s) 3, 292, 592
NspI RCATGY 2 cut(s) 293, 772
PagI TCATGA 1 cut(s) 1339
PciI ACATGT 2 cut(s) 289, 768
PmaCI CACGTG 1 cut(s) 237
PmlI CACGTG 1 cut(s) 237
Ppu21I YACGTR 1 cut(s) 237
PscI ACATGT 2 cut(s) 289, 768
PshAI GACNNNNGTC 2 cut(s) 905, 997
PsiI TTATAA 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 498
PspCI CACGTG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 487, 1202
PspPI GGNCC 2 cut(s) 486, 1200
PstI CTGCAG 1 cut(s) 465
PsuI RGATCY 1 cut(s) 936
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
RseI CAYNNNNRTG 2 cut(s) 265, 1067
SacI GAGCTC 1 cut(s) 498
SaqAI TTAA 4 cut(s) 417, 455, 681, 1239
Sau3AI GATC 1 cut(s) 936
Sau96I GGNCC 2 cut(s) 486, 1200
ScaI AGTACT 1 cut(s) 63
SduI GDGCHC 2 cut(s) 498, 554
SfaNI GCATC 2 cut(s) 269, 706
SfcI CTRYAG 2 cut(s) 372, 461
SmiMI CAYNNNNRTG 2 cut(s) 265, 1067
SmlI CTYRAG 2 cut(s) 416, 529
SmoI CTYRAG 2 cut(s) 416, 529
SpeI ACTAGT 1 cut(s) 64
Sse9I AATT 8 cut(s) 22, 271, 345, 696, 821, 1041, 1215, 1331
SsiI CCGC 1 cut(s) 153
SspI AATATT 2 cut(s) 385, 434
SspMI CTAG 4 cut(s) 65, 230, 246, 1191
SstI GAGCTC 1 cut(s) 498
StyI CCWWGG 1 cut(s) 1248
TaaI ACNGT 2 cut(s) 745, 889
TaiI ACGT 3 cut(s) 107, 239, 1061
TasI AATT 8 cut(s) 22, 271, 345, 696, 821, 1041, 1215, 1331
TatI WGTACW 1 cut(s) 61
TauI GCSGC 1 cut(s) 155
Tru1I TTAA 4 cut(s) 417, 455, 681, 1239
Tru9I TTAA 4 cut(s) 417, 455, 681, 1239
TscAI CASTG 4 cut(s) 838, 1158, 1273, 1281
TseFI GTSAC 3 cut(s) 3, 292, 592
TseI GCWGC 9 cut(s) 83, 116, 221, 401, 463, 788, 1091, 1094, 1231
Tsp45I GTSAC 3 cut(s) 3, 292, 592
TspDTI ATGAA 3 cut(s) 383, 1294, 1328
TspRI CASTG 4 cut(s) 838, 1158, 1273, 1281
Vha464I CTTAAG 1 cut(s) 416
VneI GTGCAC 1 cut(s) 550
XapI RAATTY 3 cut(s) 22, 1215, 1331
XceI RCATGY 2 cut(s) 293, 772
XspI CTAG 4 cut(s) 65, 230, 246, 1191
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.