FvH4_2g22810

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008556
Physical Location & Seq
Reverse (-)
18739403 .. 18743251
3849 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22810.t2

Sequence Viewer

Length: 2313 bp
ATGACGACTGGGATGGTTCAAGATCAGCAGAACCTTGAGAAACAGATGGGGTGCATGGCCGGCTTGCTTCAGATCTTCGATCGCCACCAGATTCTCTCCGGCAAACGCCTCTACTCCACTAAACGCCTTCCTTCCTCGGCGGCTATGGTGGCGTCGTCACCGGAGCCGTCGTCCAACGTATCGCCGGAGACATCAAAGGAGTTGGAAAAGCAACCACCGCAAAACAAATCCCTGCCTTCACCCGACAGGTTGAACTTGAAGCAGTCTCCATCGGCGTCGGAGCTCCGATCTCCTTTACCGGAGCCGAGGACGCCGACGGCCGAATCGCCTTATCCGAAATCTCCGCTTCCTCTTCCGGTTTTCGAATTCAAAGAAGGCACGAGGTCGTCGTGGAAGTTTTCGAGAGACATGCCGCGGCTCTCGCTCGATAGCAGGGCCACAGTGGACGCCAAAGGAAGCCTTTACCCGAAGGAGATCCGAACCAACGCCGCGATATTGTCCGCCAACCGAAGCGTGAACTCCGGCGAGGATGGAGACAAGAATCACCGCTCGCCTAGCGTCATTGCGAGGCTAATGGGACTGGAACCGTTACCGCAATCGGATCCTGAACCGATCAAGCTCCGGAGATCGGCTTCCGAGTCGAGAGTGAACAGAGATCTGTACCAGCCACGGTCTAACGATAACAATAATTTCCAGGCAAGGCAACCGCAGCAAGAGAATTCGAAGAGCAATGTTTCCGGCAATGTAGGCAATAAAGAGCACCGGAGCTCCAATGGTAGACAGGTAGATCCGAAGGCTTACAATGTGAAAGCTCAAAACAGGGGAATGGTGCAGAGGAAGAGCTTCTTTGACTCGGCGGATTTTTTCCCGGAGCCGAAACAGTCCATTTCGGTTTACGGCGAGATTGAGAAGCGGCTGAGGATGAGAGGCATTGATGAACCGTCTAAAGATTTGGAGACATTGAAGCAAATCCTGGAAGCGTTACAGCTCAAAGGTCTTCTTCACTCCCGGAAATCATCGTCGTCTCCCCAAATCAACTCCCGAAACTTCGTCTACGACCGGTTCGCGGAGTCGCCAATTGTGGTGATGAGGCCGTCCAGATCGCCGAGTTCCACTAACCGAGCCGCCAGATTCGGCAACGAATCGCCGCCTTCGAGTTTCCGGTCAAGAGCTGCAGCTCACCGGAATTCGAATCCCGGCGGCGAGACGTATCAGACGGCGAGCCCAAGGCGTGAGCGGCCGGAGAATGTGCGGAGCCAGAGCAGAGGTAGAAGTCCGAACCCGCTGACCAGGAGCGAGAATGGCGTGAGGAGTCCGAGTCGGAGAGGCCCATTAGTGATCGAGACGCATAGAAAAGGAAACAGCGATCCAGTGGAGCACAGGAGAGTCTCTCCGGTTCACTCTCCCAAGGTTAGTTCAAGACGGTCGGTCTCAGATCAACCAATGACCAATCGTTCACCCAGGGTCAAGAAACCAACGGCGGAGATCACACCCAAGGAGGAAAAAATAACCATCCACAGAGCAGAGGATGATTCCTCCACCATGTCCGAGAGTAGCATTAGTAATAGTACTTCTTCACAAACCGACACTGAGAGATGGAAGGTGGAGGAGTACAAAGAAGGGAGGAGTCTATTGGAGAGGTGTGACAAGCTACTACACAGCATAGCCGAGTTGCAACCGAGTCCGGTCTCCGTTCTCGACTCGTCGTTTTACAAAGAGGAATCTTCACCCTCGCCAGTGATGAAGCGCAGCATTGATTTCAAAGGTGAGATGGTAGATATTTGGGGGCAGCCAATCTGCGGAAACGAGTCGGAATCCGACGACTCTGATTTTGTCTACGTTTCCGAGATTCTCCGAGCTTTGAATTACTTACCGGAAGACTCCGACATTTTCTCATTGCTGGAGAAGCAGCAAGAGCTCAAGGGGAAAGACACTTCTCGAGTTTCGACACTCGAGAGGAGGCTCATCTTCGACATTATCACAAAAATTATCGACCGCAACCGGCAATTGCCGCCGTGGAAGGCAGTGTCACGGCAGACTTCGGTGCCGGAAATCTGGTCGGAGTTTCGGAGAATCCGGGTGAGGGATGAATCGGAGGATTTGTTTGAAGTCATATGCGGAGTATTGAAGAAGGACCTTGCAGAGGACGCAATAAACGGGTGGGGAGACTGTTCCGTCGAAATGTCCGACGCCGTTTTGGACATTGAACGCCTCATCTTCAAGGACTTGATCGGCGAAACGATCCGAGATCTCGCTTCTATTTCCCCGAATTCCGGTAGAGTTGCGGCGCTTTGTAGGAAGTTGGTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

771

Amino Acids

86.65

Weight (kDa)

9.35

Isoelectric Point (pI)

77.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VARLMGL PF14383 180 - 200 4.8e-07 DUF761-associated sequence motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2174
AccB1I GGYRCC 1 cut(s) 2044
AccBSI CCGCTC 2 cut(s) 549, 1237
AccI GTMKAC 3 cut(s) 778, 1053, 1836
AccII CGCG 3 cut(s) 415, 491, 1067
AccIII TCCGGA 1 cut(s) 621
AclWI GGATC 6 cut(s) 469, 596, 609, 782, 1361, 2236
AcoI YGGCCR 3 cut(s) 57, 318, 1238
AcsI RAATTY 4 cut(s) 365, 718, 1186, 2269
AcuI CTGAAG 1 cut(s) 53
AcyI GRCGYC 5 cut(s) 152, 275, 311, 447, 2190
AfaI GTAC 3 cut(s) 662, 1570, 1613
AfiI CCNNNNNNNGG 6 cut(s) 628, 1066, 1799, 2083, 2143, 2273
AgeI ACCGGT 1 cut(s) 1059
AhdI GACNNNNNGTC 1 cut(s) 1427
AjnI CCWGG 4 cut(s) 693, 972, 1289, 1460
Alw21I GWGCWC 5 cut(s) 285, 762, 770, 1380, 1920
AlwI GGATC 6 cut(s) 469, 596, 609, 782, 1361, 2236
Ama87I CYCGRG 2 cut(s) 1938, 1952
Aor13HI TCCGGA 1 cut(s) 621
AoxI GGCC 6 cut(s) 57, 318, 435, 1091, 1238, 1327
ApeKI GCWGC 6 cut(s) 709, 1172, 1175, 1749, 1789, 1909
ApoI RAATTY 4 cut(s) 365, 718, 1186, 2269
ArsI GACNNNNNNTTYG 2 cut(s) 1814, 1846
AsiGI ACCGGT 1 cut(s) 1059
Asp700I GAANNNNTTC 1 cut(s) 842
AspLEI GCGC 2 cut(s) 1749, 2290
AspS9I GGNCC 3 cut(s) 435, 1328, 2134
AsuC2I CCSGG 4 cut(s) 869, 1009, 1197, 2078
AsuHPI GGTGA 9 cut(s) 150, 231, 536, 1096, 1172, 1449, 1719, 1778, 2092
AsuII TTCGAA 3 cut(s) 363, 722, 1190
AvaI CYCGRG 2 cut(s) 1938, 1952
AvaII GGWCC 1 cut(s) 2134
BamHI GGATCC 1 cut(s) 601
BanI GGYRCC 1 cut(s) 2044
BanII GRGCYC 4 cut(s) 285, 770, 1226, 1920
BarI GAAGNNNNNNTAC 2 cut(s) 1555, 1587
BauI CACGAG 1 cut(s) 379
BbsI GAAGAC 3 cut(s) 989, 1884, 2299
Bbv12I GWGCWC 5 cut(s) 285, 762, 770, 1380, 1920
BbvCI CCTCAGC 1 cut(s) 917
BbvI GCAGC 6 cut(s) 721, 1159, 1187, 1761, 1801, 1921
BccI CCATC 7 cut(s) 7, 40, 277, 524, 1520, 1590, 1765
BceAI ACGGC 9 cut(s) 151, 333, 913, 1078, 1233, 1494, 1999, 2048, 2177
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BciT130I CCWGG 4 cut(s) 695, 974, 1291, 1462
BcnI CCSGG 4 cut(s) 869, 1009, 1197, 2078
BfaI CTAG 1 cut(s) 555
BfmI CTRYAG 1 cut(s) 1173
BfoI RGCGCY 1 cut(s) 2291
BglII AGATCT 3 cut(s) 72, 655, 2248
BmcAI AGTACT 1 cut(s) 1570
Bme1390I CCNGG 8 cut(s) 695, 869, 974, 1009, 1197, 1291, 1462, 2078
Bme18I GGWCC 1 cut(s) 2134
BmeRI GACNNNNNGTC 1 cut(s) 1427
BmeT110I CYCGRG 2 cut(s) 1938, 1952
BmgT120I GGNCC 3 cut(s) 435, 1328, 2134
BmiI GGNNCC 7 cut(s) 165, 303, 585, 603, 873, 1256, 2046
BmrFI CCNGG 8 cut(s) 695, 869, 974, 1009, 1197, 1291, 1462, 2078
BmrI ACTGGG 1 cut(s) 18
BmuI ACTGGG 1 cut(s) 18
BpiI GAAGAC 3 cut(s) 989, 1884, 2299
BplI GAGNNNNNCTC 2 cut(s) 1375, 1407
BpmI CTGGAG 1 cut(s) 1922
Bpu10I CCTNAGC 1 cut(s) 917
Bpu14I TTCGAA 3 cut(s) 363, 722, 1190
BpuEI CTTGAG 2 cut(s) 56, 1904
BpuMI CCSGG 4 cut(s) 869, 1009, 1197, 2078
BsaHI GRCGYC 5 cut(s) 152, 275, 311, 447, 2190
BsaI GGTCTC 2 cut(s) 1435, 1693
BsaXI ACNNNNNCTCC 8 cut(s) 155, 185, 752, 782, 1303, 1333, 2087, 2117
Bsc4I CCNNNNNNNGG 6 cut(s) 628, 1066, 1799, 2083, 2143, 2273
Bse118I RCCGGY 3 cut(s) 59, 1059, 2001
Bse1I ACTGG 4 cut(s) 13, 585, 1370, 1736
Bse3DI GCAATG 4 cut(s) 561, 736, 748, 1895
BseAI TCCGGA 1 cut(s) 621
BseBI CCWGG 4 cut(s) 695, 974, 1291, 1462
BseGI GGATG 6 cut(s) 18, 535, 927, 1512, 1534, 2092
BseLI CCNNNNNNNGG 6 cut(s) 628, 1066, 1799, 2083, 2143, 2273
BseMI GCAATG 4 cut(s) 561, 736, 748, 1895
BseMII CTCAG 3 cut(s) 908, 1446, 1581
BseNI ACTGG 4 cut(s) 13, 585, 1370, 1736
BseRI GAGGAG 4 cut(s) 1324, 1622, 1639, 1972
BseX3I CGGCCG 2 cut(s) 318, 1238
BseXI GCAGC 6 cut(s) 721, 1159, 1187, 1761, 1801, 1921
BsgI GTGCAG 1 cut(s) 851
Bsh1236I CGCG 3 cut(s) 415, 491, 1067
Bsh1285I CGRYCG 6 cut(s) 82, 321, 1060, 1241, 1427, 1996
BshFI GGCC 6 cut(s) 59, 320, 437, 1093, 1240, 1329
BshNI GGYRCC 1 cut(s) 2044
BshTI ACCGGT 1 cut(s) 1059
BsiEI CGRYCG 6 cut(s) 82, 321, 1060, 1241, 1427, 1996
BsiHKAI GWGCWC 5 cut(s) 285, 762, 770, 1380, 1920
BsiHKCI CYCGRG 2 cut(s) 1938, 1952
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 6 cut(s) 628, 1066, 1799, 2083, 2143, 2273
BsmBI CGTCTC 3 cut(s) 1029, 1199, 1337
BsmFI GGGAC 1 cut(s) 591
BsnI GGCC 6 cut(s) 59, 320, 437, 1093, 1240, 1329
Bso31I GGTCTC 2 cut(s) 1435, 1693
BsoBI CYCGRG 2 cut(s) 1938, 1952
Bsp119I TTCGAA 3 cut(s) 363, 722, 1190
Bsp1286I GDGCHC 6 cut(s) 285, 762, 770, 1226, 1380, 1920
Bsp13I TCCGGA 1 cut(s) 621
BspANI GGCC 6 cut(s) 59, 320, 437, 1093, 1240, 1329
BspCNI CTCAG 3 cut(s) 909, 1445, 1582
BspEI TCCGGA 1 cut(s) 621
BspFNI CGCG 3 cut(s) 415, 491, 1067
BspLI GGNNCC 7 cut(s) 165, 303, 585, 603, 873, 1256, 2046
BspMAI CTGCAG 1 cut(s) 1177
BspPI GGATC 6 cut(s) 469, 596, 609, 782, 1361, 2236
BspQI GCTCTTC 2 cut(s) 719, 833
BspT104I TTCGAA 3 cut(s) 363, 722, 1190
BspT107I GGYRCC 1 cut(s) 2044
BspTNI GGTCTC 2 cut(s) 1435, 1693
BsrBI CCGCTC 2 cut(s) 549, 1237
BsrDI GCAATG 4 cut(s) 561, 736, 748, 1895
BsrFI RCCGGY 3 cut(s) 59, 1059, 2001
BsrI ACTGG 4 cut(s) 13, 585, 1370, 1736
BssAI RCCGGY 3 cut(s) 59, 1059, 2001
BssNI GRCGYC 5 cut(s) 152, 275, 311, 447, 2190
BssSI CACGAG 1 cut(s) 379
BssT1I CCWWGG 3 cut(s) 1226, 1407, 1494
Bst2BI CACGAG 1 cut(s) 379
Bst2UI CCWGG 4 cut(s) 695, 974, 1291, 1462
Bst4CI ACNGT 7 cut(s) 442, 588, 672, 882, 942, 1425, 2171
Bst6I CTCTTC 3 cut(s) 357, 719, 833
BstACI GRCGYC 5 cut(s) 152, 275, 311, 447, 2190
BstBI TTCGAA 3 cut(s) 363, 722, 1190
BstC8I GCNNGC 4 cut(s) 61, 65, 551, 1222
BstDEI CTNAG 3 cut(s) 917, 1432, 1590
BstDSI CCRYGG 3 cut(s) 413, 668, 2015
BstENI CCTNNNNNAGG 1 cut(s) 2141
BstF5I GGATG 6 cut(s) 18, 535, 927, 1512, 1534, 2092
BstFNI CGCG 3 cut(s) 415, 491, 1067
BstH2I RGCGCY 1 cut(s) 2291
BstHHI GCGC 2 cut(s) 1749, 2290
BstMCI CGRYCG 6 cut(s) 82, 321, 1060, 1241, 1427, 1996
BstNI CCWGG 4 cut(s) 695, 974, 1291, 1462
BstNSI RCATGY 1 cut(s) 412
BstSCI CCNGG 8 cut(s) 693, 867, 972, 1007, 1195, 1289, 1460, 2076
BstSFI CTRYAG 1 cut(s) 1173
BstUI CGCG 3 cut(s) 415, 491, 1067
BstV1I GCAGC 6 cut(s) 721, 1159, 1187, 1761, 1801, 1921
BstV2I GAAGAC 3 cut(s) 989, 1884, 2299
BstX2I RGATCY 6 cut(s) 72, 474, 601, 655, 787, 2248
BstYI RGATCY 6 cut(s) 72, 474, 601, 655, 787, 2248
BstZI CGGCCG 2 cut(s) 318, 1238
BsuRI GGCC 6 cut(s) 59, 320, 437, 1093, 1240, 1329
BtgI CCRYGG 3 cut(s) 413, 668, 2015
BtsCI GGATG 6 cut(s) 18, 535, 927, 1512, 1534, 2092
BtsI GCAGTG 1 cut(s) 2031
BtsIMutI CAGTG 5 cut(s) 447, 1377, 1587, 1743, 2031
Cac8I GCNNGC 4 cut(s) 61, 65, 551, 1222
CfoI GCGC 2 cut(s) 1749, 2290
Cfr10I RCCGGY 3 cut(s) 59, 1059, 2001
Cfr13I GGNCC 3 cut(s) 435, 1328, 2134
Cfr42I CCGCGG 1 cut(s) 416
CseI GACGC 8 cut(s) 141, 264, 319, 455, 547, 1354, 2156, 2198
Csp6I GTAC 3 cut(s) 661, 1569, 1612
CspAI ACCGGT 1 cut(s) 1059
CviAII CATG 3 cut(s) 55, 409, 1543
CviQI GTAC 3 cut(s) 661, 1569, 1612
DdeI CTNAG 3 cut(s) 917, 1432, 1590
DrdI GACNNNNNNGTC 1 cut(s) 2174
DriI GACNNNNNGTC 1 cut(s) 1427
DseDI GACNNNNNNGTC 1 cut(s) 2174
EaeI YGGCCR 3 cut(s) 57, 318, 1238
EagI CGGCCG 2 cut(s) 318, 1238
Eam1104I CTCTTC 3 cut(s) 357, 719, 833
Eam1105I GACNNNNNGTC 1 cut(s) 1427
EarI CTCTTC 3 cut(s) 357, 719, 833
EciI GGCGGA 3 cut(s) 490, 872, 1496
Ecl136II GAGCTC 3 cut(s) 283, 768, 1918
EclXI CGGCCG 2 cut(s) 318, 1238
Eco130I CCWWGG 3 cut(s) 1226, 1407, 1494
Eco24I GRGCYC 4 cut(s) 285, 770, 1226, 1920
Eco31I GGTCTC 2 cut(s) 1435, 1693
Eco47I GGWCC 1 cut(s) 2134
Eco52I CGGCCG 2 cut(s) 318, 1238
Eco53kI GAGCTC 3 cut(s) 283, 768, 1918
Eco57I CTGAAG 1 cut(s) 53
Eco88I CYCGRG 2 cut(s) 1938, 1952
EcoICRI GAGCTC 3 cut(s) 283, 768, 1918
EcoNI CCTNNNNNAGG 1 cut(s) 2141
EcoO109I RGGNCCY 1 cut(s) 2134
EcoRI GAATTC 4 cut(s) 365, 718, 1186, 2269
EcoRII CCWGG 4 cut(s) 693, 972, 1289, 1460
EcoT14I CCWWGG 3 cut(s) 1226, 1407, 1494
EcoT38I GRGCYC 4 cut(s) 285, 770, 1226, 1920
ErhI CCWWGG 3 cut(s) 1226, 1407, 1494
Esp3I CGTCTC 3 cut(s) 1029, 1199, 1337
FaeI CATG 3 cut(s) 58, 412, 1546
FaiI YATR 8 cut(s) 56, 146, 410, 1350, 1544, 1664, 2114, 2116
FalI AAGNNNNNCTT 7 cut(s) 444, 476, 830, 862, 984, 1016, 2291
FaqI GGGAC 1 cut(s) 591
FatI CATG 3 cut(s) 54, 408, 1542
FauI CCCGC 1 cut(s) 1290
FauNDI CATATG 1 cut(s) 2114
FblI GTMKAC 3 cut(s) 778, 1053, 1836
FokI GGATG 6 cut(s) 25, 542, 934, 1499, 1541, 2099
FriOI GRGCYC 4 cut(s) 285, 770, 1226, 1920
FspBI CTAG 1 cut(s) 555
GlaI GCGC 2 cut(s) 1748, 2289
GsuI CTGGAG 1 cut(s) 1922
HaeII RGCGCY 1 cut(s) 2291
HaeIII GGCC 6 cut(s) 59, 320, 437, 1093, 1240, 1329
HgaI GACGC 8 cut(s) 141, 264, 319, 455, 547, 1354, 2156, 2198
HhaI GCGC 2 cut(s) 1749, 2290
Hin1I GRCGYC 5 cut(s) 152, 275, 311, 447, 2190
Hin1II CATG 3 cut(s) 58, 412, 1546
Hin6I GCGC 2 cut(s) 1747, 2288
HinP1I GCGC 2 cut(s) 1747, 2288
HphI GGTGA 9 cut(s) 150, 231, 536, 1096, 1172, 1449, 1719, 1778, 2092
Hpy166II GTNNAC 9 cut(s) 445, 517, 649, 779, 895, 1054, 1399, 1457, 1837
Hpy8I GTNNAC 9 cut(s) 445, 517, 649, 779, 895, 1054, 1399, 1457, 1837
HpyCH4III ACNGT 7 cut(s) 442, 588, 672, 882, 942, 1425, 2171
HpyCH4IV ACGT 3 cut(s) 177, 1208, 1839
HpyCH4V TGCA 5 cut(s) 54, 832, 1175, 1675, 2141
HpyF3I CTNAG 3 cut(s) 917, 1432, 1590
HpySE526I ACGT 3 cut(s) 177, 1208, 1839
Hsp92I GRCGYC 5 cut(s) 152, 275, 311, 447, 2190
Hsp92II CATG 3 cut(s) 58, 412, 1546
HspAI GCGC 2 cut(s) 1747, 2288
Kpn2I TCCGGA 1 cut(s) 621
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
KspI CCGCGG 1 cut(s) 416
LguI GCTCTTC 2 cut(s) 719, 833
Lsp1109I GCAGC 6 cut(s) 721, 1159, 1187, 1761, 1801, 1921
MaeI CTAG 1 cut(s) 555
MaeII ACGT 3 cut(s) 177, 1208, 1839
MaeIII GTNAC 5 cut(s) 156, 588, 981, 1643, 2028
MbiI CCGCTC 2 cut(s) 549, 1237
MfeI CAATTG 2 cut(s) 1077, 2006
MflI RGATCY 6 cut(s) 72, 474, 601, 655, 787, 2248
MhlI GDGCHC 6 cut(s) 285, 762, 770, 1226, 1380, 1920
MluCI AATT 9 cut(s) 365, 688, 718, 1077, 1186, 1864, 1986, 2006, 2269
MmeI TCCRAC 9 cut(s) 183, 198, 258, 1301, 1791, 1842, 1908, 2040, 2211
MroI TCCGGA 1 cut(s) 621
MroNI GCCGGC 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 842
MspA1I CMGCKG 2 cut(s) 415, 1285
MspR9I CCNGG 8 cut(s) 695, 869, 974, 1009, 1197, 1291, 1462, 2078
MunI CAATTG 2 cut(s) 1077, 2006
MvaI CCWGG 4 cut(s) 695, 974, 1291, 1462
MvnI CGCG 3 cut(s) 415, 491, 1067
NaeI GCCGGC 1 cut(s) 61
NciI CCSGG 4 cut(s) 869, 1009, 1197, 2078
NdeI CATATG 1 cut(s) 2114
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 3 cut(s) 58, 412, 1546
NlaIV GGNNCC 7 cut(s) 165, 303, 585, 603, 873, 1256, 2046
NmeAIII GCCGAG 5 cut(s) 116, 330, 833, 1131, 1693
NmuCI GTSAC 3 cut(s) 156, 1643, 2028
NspI RCATGY 1 cut(s) 412
NspV TTCGAA 3 cut(s) 363, 722, 1190
PaeR7I CTCGAG 2 cut(s) 1938, 1952
PasI CCCWGGG 1 cut(s) 1461
PciSI GCTCTTC 2 cut(s) 719, 833
PcsI WCGNNNNNNNCGW 4 cut(s) 332, 386, 1151, 2184
PdiI GCCGGC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 842
PfoI TCCNGGA 3 cut(s) 867, 972, 1007
PinAI ACCGGT 1 cut(s) 1059
Ple19I CGATCG 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 2134
Psp124BI GAGCTC 3 cut(s) 285, 770, 1920
Psp5II RGGWCCY 1 cut(s) 2134
Psp6I CCWGG 4 cut(s) 693, 972, 1289, 1460
PspGI CCWGG 4 cut(s) 693, 972, 1289, 1460
PspN4I GGNNCC 7 cut(s) 165, 303, 585, 603, 873, 1256, 2046
PspPI GGNCC 3 cut(s) 435, 1328, 2134
PspPPI RGGWCCY 1 cut(s) 2134
PstI CTGCAG 1 cut(s) 1177
PsuI RGATCY 6 cut(s) 72, 474, 601, 655, 787, 2248
PvuI CGATCG 1 cut(s) 82
RsaI GTAC 3 cut(s) 662, 1570, 1613
RsaNI GTAC 3 cut(s) 661, 1569, 1612
SacI GAGCTC 3 cut(s) 285, 770, 1920
SacII CCGCGG 1 cut(s) 416
SapI GCTCTTC 2 cut(s) 719, 833
Sau96I GGNCC 3 cut(s) 435, 1328, 2134
ScaI AGTACT 1 cut(s) 1570
ScrFI CCNGG 8 cut(s) 695, 869, 974, 1009, 1197, 1291, 1462, 2078
SduI GDGCHC 6 cut(s) 285, 762, 770, 1226, 1380, 1920
SfcI CTRYAG 1 cut(s) 1173
Sfr274I CTCGAG 2 cut(s) 1938, 1952
Sfr303I CCGCGG 1 cut(s) 416
SfuI TTCGAA 3 cut(s) 363, 722, 1190
SgrBI CCGCGG 1 cut(s) 416
SinI GGWCC 1 cut(s) 2134
SlaI CTCGAG 2 cut(s) 1938, 1952
SmlI CTYRAG 4 cut(s) 35, 1919, 1938, 1952
SmoI CTYRAG 4 cut(s) 35, 1919, 1938, 1952
Sse9I AATT 9 cut(s) 365, 688, 718, 1077, 1186, 1864, 1986, 2006, 2269
SspMI CTAG 1 cut(s) 555
SstI GAGCTC 3 cut(s) 285, 770, 1920
StyD4I CCNGG 8 cut(s) 693, 867, 972, 1007, 1195, 1289, 1460, 2076
StyI CCWWGG 3 cut(s) 1226, 1407, 1494
TaaI ACNGT 7 cut(s) 442, 588, 672, 882, 942, 1425, 2171
TaiI ACGT 3 cut(s) 180, 1211, 1842
TaqII GACCGA 1 cut(s) 1417
TasI AATT 9 cut(s) 365, 688, 718, 1077, 1186, 1864, 1986, 2006, 2269
TatI WGTACW 2 cut(s) 1568, 1611
TscAI CASTG 5 cut(s) 447, 1377, 1594, 1743, 2031
TseFI GTSAC 3 cut(s) 156, 1643, 2028
TseI GCWGC 6 cut(s) 709, 1172, 1175, 1749, 1789, 1909
Tsp45I GTSAC 3 cut(s) 156, 1643, 2028
TspDTI ATGAA 3 cut(s) 951, 1757, 2103
TspGWI ACGGA 2 cut(s) 1681, 2164
TspRI CASTG 5 cut(s) 447, 1377, 1594, 1743, 2031
VpaK11BI GGWCC 1 cut(s) 2134
XagI CCTNNNNNAGG 1 cut(s) 2141
XapI RAATTY 4 cut(s) 365, 718, 1186, 2269
XceI RCATGY 1 cut(s) 412
XhoI CTCGAG 2 cut(s) 1938, 1952
XmiI GTMKAC 3 cut(s) 778, 1053, 1836
XmnI GAANNNNTTC 1 cut(s) 842
XspI CTAG 1 cut(s) 555
ZrmI AGTACT 1 cut(s) 1570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.