MD11G1277100.v1.1

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008556
Physical Location & Seq
Reverse (-)
39427348 .. 39431630
4283 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1277100.v1.1.491

Sequence Viewer

Length: 2304 bp
ATGACGACTGGAAGAATGGTGCAAGAACAGAACCTGGAGAAGCAGATAGAGAAACAGATGGGGTGCATGGCTGGCTTCCTCCAGATCTTCGATCGCCACCAGCTTCTCAACGGAAAACGCCTCTACTCCACCAAACGCCTCCCTCCCTCGGCGACGGTGGTATCGTCGCCAGAGTCGGAGTCGGCCATGTCGCCGGAGATATCCAAAGAATTGGAAAAACAACTGCAGAACGAGACGGCACCGTCTCCGGACCGATCGAAGCAATCTCCATCTCCGACGAAGGATCTCCGATCTCCATCACCAGAAACTCGCGCTCCCCCGTCACCGGAACCTCATCCGAAATCTCCTCTTCCGCTTCCAGTTTTCGAATTCAAAGAAGGAACGAGGTCCTCGTGGAAGTTCTCCAGAGAAGCGCCGAGGCTCTCACTGGATAGCAGAGCCACCGTCGACGCCAAGGGAAGCCTATACCCGAGGGAGATTCGAACAAACACCGCCATACTAACCGCCGGTCGGAGCTCCGCCGACGGAGGAAGCGATAGCGACAAGCAACACCGATCACCGAGCGTTATCGCTAGGCTAATGGGACTCGAACCGTTGCAGCAGTCGGATCCGGAACCGATTAAGCTCCGGAGGTCTTCGTCCGAATCCAGAGTGAACCGAGACCTGAACCAGTACCGGTTCGTCGACGGCAACAATTACCAGCAGAAGCAACCGCCGCCACAGAATGTGCAGGGCCATAATTTCTCCGGCAATGTAGGCAAAGACGACCGGTGCTCCAATCGGGGTTCCTATCCGAGGCAGCCGGATCCGAAGAACAATGCGAAGGTTCAGAGCAAAGGAATGGCGCAGAGAAAGTGCTTCTTCAACTCGGCGGACTTCTTCCCCGAGCCGAAGCAGAGAGCCTCCGTTTACAACGAGATTGAGAAGCGGTTGAAGATGCGAGGAATCGACGAGCCGTCGAAAGATTTGGAGACGTTGAAGGAAATCTTCGAAGCTCTGCAGCTCAAAGGCCTCCTGCGCTCCAGGAAAGCTGCCAATCAGATTAATTCCAGAAACTTCTTCTACGATCGGAGCTTCCAGTCGCCGACCGTAGTGATGAAGCCGTCCATATCGCCGAGCTCGGTTCACCGCGCGGCCATATTCGGAAACGACTCGCCGCCGCCAAATTCGGGATTTAGGTCGAGAGCCGGAGTTCGCCGAAACGGCGAGGTATCACCTGCGGTGAGTCCTAGGCGCGACCGGCCGGAGAATGCACGGAGCCAGACTAGAGGGAGGAGTCCTGCCGCGCGAGAGGGCGGCGTGAGGAGTCCGAGTCGGAGAGGAGGACCGTTATCGATCGAAACGCAGCGGAAGGGGAATGATTCGGTGGAGCAGAGGAGAGTATCTCCGGTGCATTCTCCTAAAGTCAGTTCGAGAGGACCAGATCAAACGCCCACGAATCGATCTCCCAGGTACATTAAGAAACCAACGGTTGAGATTGCTCTAAGTGAGGAAAAGGTGTTCCATCGGGCGGAGGACGAATCGTCCACCACCATGTCAGAGAGCGGCATTAGGAACAGCACCTCGTCACAAGCCGACACAGAGAGGTGGAAGGCGGAAGAGTACAAAGAAGGGAGGAGTTTGTTGGAGAGGTGTGATAAGTTGCTTCACAGCATAGCTGAGTTGCAACCGAGTCCTGTCTCGGTTCTCGACTCGTCGTTTTACAAGGACGAATCGTCGCCTTCGCCTGTCATGAAACGCAGCATTGATTTCAAAGTTGAAATGGAAGACATATGGGGGGAGGCAATGTGGGGCACTGAGTCCGAATCCGAAGACAGCGATTTCGTCTACGTATCGGAGATCTTGCGGGCTTCCACTTACTTACCGGAAGACTCCGACATTTTCCTATTACTAGAAAAGAAGCAACATTTCAAGGGAAAAGAGACCTCCAAAGAGTCAACACTCGAGAGAAGGATCATTTTCGACACCGTCACCGAAATTCTAGACCGAAACCGTCACTTGCCGCCGTGGAAGGCGGTCTCGTGGCAGACTTCCTTGCCCGAAACTTGGTCCGAGTTTCAGAGAATCCGGGAGAGGGACGACTCCGAGGACTTGTTCGAGGTCATATGCGGGATGTTGAAAAAGGACCTTGCCGGCGACGCGATCAACGGATGGGGAGGTTGCTCCATCGAAATGTCCGACGCCGTTTTGGACATCGAACGGCTCATTTTCAAGGACTTGATCGGGGAAACGATCCGAGATCTCGCCGCTTTTCCCACCAGTTATATCAAAGTTTCGGCGCCTTGTAGGAAGTTGGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

768

Amino Acids

86.5

Weight (kDa)

9.1

Isoelectric Point (pI)

77.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 607 - 743 1.2e-14 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1225
AasI GACNNNNNNGTC 1 cut(s) 241
Acc36I ACCTGC 1 cut(s) 1225
AccB1I GGYRCC 2 cut(s) 238, 2278
AccBSI CCGCTC 1 cut(s) 1545
AccI GTMKAC 3 cut(s) 447, 684, 1827
AccII CGCG 7 cut(s) 312, 1131, 1133, 1236, 1286, 1288, 2141
AccIII TCCGGA 3 cut(s) 247, 610, 627
AclWI GGATC 7 cut(s) 291, 602, 615, 800, 813, 1961, 2227
AcoI YGGCCR 3 cut(s) 183, 1134, 1241
AcsI RAATTY 3 cut(s) 368, 1165, 1977
AcyI GRCGYC 3 cut(s) 450, 2181, 2279
AfaI GTAC 3 cut(s) 674, 1454, 1604
AfiI CCNNNNNNNGG 8 cut(s) 148, 325, 510, 795, 1169, 1510, 2046, 2074
AgeI ACCGGT 2 cut(s) 675, 768
AgsI TTSAA 9 cut(s) 373, 865, 934, 979, 1753, 1760, 1912, 2119, 2212
AhdI GACNNNNNGTC 3 cut(s) 955, 1522, 1714
AjnI CCWGG 3 cut(s) 33, 1022, 1448
AloI GAACNNNNNNTCC 2 cut(s) 1484, 1516
AluBI AGCT 9 cut(s) 103, 516, 625, 995, 1003, 1031, 1074, 1119, 1658
AluI AGCT 9 cut(s) 103, 516, 625, 995, 1003, 1031, 1074, 1119, 1658
Alw21I GWGCWC 3 cut(s) 518, 776, 1121
Alw26I GTCTC 7 cut(s) 227, 249, 654, 965, 1684, 1916, 2023
AlwI GGATC 7 cut(s) 291, 602, 615, 800, 813, 1961, 2227
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 3 cut(s) 469, 884, 1943
Aor13HI TCCGGA 3 cut(s) 247, 610, 627
AoxI GGCC 5 cut(s) 183, 733, 1009, 1134, 1241
ApeKI GCWGC 6 cut(s) 598, 799, 1000, 1031, 1345, 1740
ApoI RAATTY 3 cut(s) 368, 1165, 1977
ArsI GACNNNNNNTTYG 2 cut(s) 1805, 1837
AseI ATTAAT 1 cut(s) 1044
AsiGI ACCGGT 2 cut(s) 675, 768
AspA2I CCTAGG 1 cut(s) 1229
AspLEI GCGC 8 cut(s) 314, 415, 847, 1020, 1133, 1236, 1288, 2281
AspS9I GGNCC 7 cut(s) 250, 387, 733, 1325, 1418, 2049, 2125
AsuC2I CCSGG 1 cut(s) 2069
AsuHPI GGTGA 7 cut(s) 291, 315, 549, 1118, 1206, 1234, 1963
AsuII TTCGAA 3 cut(s) 366, 481, 990
AvaI CYCGRG 3 cut(s) 469, 884, 1943
AvaII GGWCC 6 cut(s) 250, 387, 1325, 1418, 2049, 2125
AvrII CCTAGG 1 cut(s) 1229
BaeGI GKGCMC 1 cut(s) 1796
BaeI ACNNNNGTAYC 2 cut(s) 144, 177
BamHI GGATCC 2 cut(s) 607, 805
BanI GGYRCC 2 cut(s) 238, 2278
BanII GRGCYC 2 cut(s) 518, 1121
BauI CACGAG 2 cut(s) 391, 2020
BbsI GAAGAC 5 cut(s) 627, 1773, 1818, 1875, 2290
Bbv12I GWGCWC 3 cut(s) 518, 776, 1121
BbvI GCAGC 6 cut(s) 610, 811, 1012, 1018, 1357, 1752
BccI CCATC 6 cut(s) 52, 277, 304, 1512, 2145, 2174
BceAI ACGGC 8 cut(s) 252, 703, 940, 1087, 1219, 1990, 2168, 2216
BciT130I CCWGG 3 cut(s) 35, 1024, 1450
BcnI CCSGG 1 cut(s) 2069
BcoDI GTCTC 7 cut(s) 227, 249, 654, 965, 1684, 1916, 2023
BfaI CTAG 5 cut(s) 573, 1230, 1266, 1892, 1982
BfmI CTRYAG 2 cut(s) 224, 998
BfoI RGCGCY 2 cut(s) 416, 2282
BfuAI ACCTGC 1 cut(s) 1225
BglI GCCNNNNNGGC 1 cut(s) 1203
BglII AGATCT 3 cut(s) 84, 1839, 2239
BlnI CCTAGG 1 cut(s) 1229
Bme1390I CCNGG 4 cut(s) 35, 1024, 1450, 2069
Bme18I GGWCC 6 cut(s) 250, 387, 1325, 1418, 2049, 2125
BmeRI GACNNNNNGTC 3 cut(s) 955, 1522, 1714
BmeT110I CYCGRG 3 cut(s) 469, 884, 1943
BmgT120I GGNCC 7 cut(s) 250, 387, 733, 1325, 1418, 2049, 2125
BmiI GGNNCC 8 cut(s) 240, 330, 609, 615, 787, 807, 1259, 2280
BmrFI CCNGG 4 cut(s) 35, 1024, 1450, 2069
BmsI GCATC 1 cut(s) 927
BpiI GAAGAC 5 cut(s) 627, 1773, 1818, 1875, 2290
BplI GAGNNNNNCTC 2 cut(s) 1369, 1401
BpmI CTGGAG 4 cut(s) 56, 65, 388, 1006
Bpu14I TTCGAA 3 cut(s) 366, 481, 990
BpuMI CCSGG 1 cut(s) 2069
Bsa29I ATCGAT 2 cut(s) 1334, 1441
BsaAI YACGTR 1 cut(s) 1831
BsaBI GATNNNNATC 1 cut(s) 295
BsaHI GRCGYC 3 cut(s) 450, 2181, 2279
BsaI GGTCTC 3 cut(s) 654, 1916, 2023
BsaJI CCNNGG 9 cut(s) 147, 416, 453, 470, 794, 1229, 1448, 2006, 2085
BsaWI WCCGGW 8 cut(s) 247, 325, 610, 627, 675, 768, 1387, 1864
BsaXI ACNNNNNCTCC 8 cut(s) 298, 328, 758, 788, 1297, 1327, 1772, 1802
Bsc4I CCNNNNNNNGG 8 cut(s) 148, 325, 510, 795, 1169, 1510, 2046, 2074
Bse118I RCCGGY 5 cut(s) 506, 675, 768, 1239, 2132
Bse1I ACTGG 6 cut(s) 13, 359, 432, 670, 1078, 2259
Bse3DI GCAATG 2 cut(s) 757, 1791
Bse8I GATNNNNATC 1 cut(s) 295
BseAI TCCGGA 3 cut(s) 247, 610, 627
BseBI CCWGG 3 cut(s) 35, 1024, 1450
BseCI ATCGAT 2 cut(s) 1334, 1441
BseDI CCNNGG 9 cut(s) 147, 416, 453, 470, 794, 1229, 1448, 2006, 2085
BseGI GGATG 3 cut(s) 334, 2118, 2156
BseJI GATNNNNATC 1 cut(s) 295
BseLI CCNNNNNNNGG 8 cut(s) 148, 325, 510, 795, 1169, 1510, 2046, 2074
BseMI GCAATG 2 cut(s) 757, 1791
BseMII CTCAG 2 cut(s) 1650, 1788
BseNI ACTGG 6 cut(s) 13, 359, 432, 670, 1078, 2259
BseRI GAGGAG 6 cut(s) 336, 1288, 1318, 1335, 1390, 1630
BseSI GKGCMC 1 cut(s) 1796
BseX3I CGGCCG 1 cut(s) 1241
BseXI GCAGC 6 cut(s) 610, 811, 1012, 1018, 1357, 1752
BsgI GTGCAG 1 cut(s) 749
Bsh1236I CGCG 7 cut(s) 312, 1131, 1133, 1236, 1286, 1288, 2141
Bsh1285I CGRYCG 9 cut(s) 94, 257, 511, 769, 1069, 1089, 1240, 1244, 1338
BshFI GGCC 5 cut(s) 185, 735, 1011, 1136, 1243
BshNI GGYRCC 2 cut(s) 238, 2278
BshTI ACCGGT 2 cut(s) 675, 768
BshVI ATCGAT 2 cut(s) 1334, 1441
BsiEI CGRYCG 9 cut(s) 94, 257, 511, 769, 1069, 1089, 1240, 1244, 1338
BsiHKAI GWGCWC 3 cut(s) 518, 776, 1121
BsiHKCI CYCGRG 3 cut(s) 469, 884, 1943
BslFI GGGAC 2 cut(s) 597, 2090
BslI CCNNNNNNNGG 8 cut(s) 148, 325, 510, 795, 1169, 1510, 2046, 2074
BsmAI GTCTC 7 cut(s) 227, 249, 654, 965, 1684, 1916, 2023
BsmBI CGTCTC 3 cut(s) 227, 249, 965
BsmFI GGGAC 2 cut(s) 597, 2090
BsmI GAATGC 2 cut(s) 1255, 1393
BsnI GGCC 5 cut(s) 185, 735, 1011, 1136, 1243
Bso31I GGTCTC 3 cut(s) 654, 1916, 2023
BsoBI CYCGRG 3 cut(s) 469, 884, 1943
Bsp119I TTCGAA 3 cut(s) 366, 481, 990
Bsp1286I GDGCHC 4 cut(s) 518, 776, 1121, 1796
Bsp13I TCCGGA 3 cut(s) 247, 610, 627
BspANI GGCC 5 cut(s) 185, 735, 1011, 1136, 1243
BspCNI CTCAG 2 cut(s) 1651, 1789
BspDI ATCGAT 2 cut(s) 1334, 1441
BspEI TCCGGA 3 cut(s) 247, 610, 627
BspFNI CGCG 7 cut(s) 312, 1131, 1133, 1236, 1286, 1288, 2141
BspHI TCATGA 1 cut(s) 1731
BspLI GGNNCC 8 cut(s) 240, 330, 609, 615, 787, 807, 1259, 2280
BspMAI CTGCAG 2 cut(s) 228, 1002
BspMI ACCTGC 1 cut(s) 1225
BspPI GGATC 7 cut(s) 291, 602, 615, 800, 813, 1961, 2227
BspT104I TTCGAA 3 cut(s) 366, 481, 990
BspT107I GGYRCC 2 cut(s) 238, 2278
BspTNI GGTCTC 3 cut(s) 654, 1916, 2023
BsrBI CCGCTC 1 cut(s) 1545
BsrDI GCAATG 2 cut(s) 757, 1791
BsrFI RCCGGY 5 cut(s) 506, 675, 768, 1239, 2132
BsrI ACTGG 6 cut(s) 13, 359, 432, 670, 1078, 2259
BssAI RCCGGY 5 cut(s) 506, 675, 768, 1239, 2132
BssECI CCNNGG 9 cut(s) 147, 416, 453, 470, 794, 1229, 1448, 2006, 2085
BssNI GRCGYC 3 cut(s) 450, 2181, 2279
BssSI CACGAG 2 cut(s) 391, 2020
BssT1I CCWWGG 2 cut(s) 453, 1229
Bst2BI CACGAG 2 cut(s) 391, 2020
Bst2UI CCWGG 3 cut(s) 35, 1024, 1450
Bst4CI ACNGT 9 cut(s) 157, 243, 445, 594, 1090, 1329, 1471, 1969, 1994
Bst6I CTCTTC 2 cut(s) 354, 1593
BstACI GRCGYC 3 cut(s) 450, 2181, 2279
BstBAI YACGTR 1 cut(s) 1831
BstBI TTCGAA 3 cut(s) 366, 481, 990
BstC8I GCNNGC 3 cut(s) 73, 1848, 2134
BstDEI CTNAG 3 cut(s) 1484, 1659, 1797
BstDSI CCRYGG 1 cut(s) 2006
BstENI CCTNNNNNAGG 1 cut(s) 793
BstF5I GGATG 3 cut(s) 334, 2118, 2156
BstFNI CGCG 7 cut(s) 312, 1131, 1133, 1236, 1286, 1288, 2141
BstH2I RGCGCY 2 cut(s) 416, 2282
BstHHI GCGC 8 cut(s) 314, 415, 847, 1020, 1133, 1236, 1288, 2281
BstMAI GTCTC 7 cut(s) 227, 249, 654, 965, 1684, 1916, 2023
BstMCI CGRYCG 9 cut(s) 94, 257, 511, 769, 1069, 1089, 1240, 1244, 1338
BstMWI GCNNNNNNNGC 7 cut(s) 72, 715, 756, 1017, 1203, 1240, 2138
BstNI CCWGG 3 cut(s) 35, 1024, 1450
BstSCI CCNGG 4 cut(s) 33, 1022, 1448, 2067
BstSFI CTRYAG 2 cut(s) 224, 998
BstSLI GKGCMC 1 cut(s) 1796
BstSNI TACGTA 1 cut(s) 1831
BstUI CGCG 7 cut(s) 312, 1131, 1133, 1236, 1286, 1288, 2141
BstV1I GCAGC 6 cut(s) 610, 811, 1012, 1018, 1357, 1752
BstV2I GAAGAC 5 cut(s) 627, 1773, 1818, 1875, 2290
BstX2I RGATCY 6 cut(s) 84, 283, 607, 805, 1839, 2239
BstXI CCANNNNNNTGG 1 cut(s) 211
BstYI RGATCY 6 cut(s) 84, 283, 607, 805, 1839, 2239
BstZI CGGCCG 1 cut(s) 1241
Bsu15I ATCGAT 2 cut(s) 1334, 1441
BsuRI GGCC 5 cut(s) 185, 735, 1011, 1136, 1243
BsuTUI ATCGAT 2 cut(s) 1334, 1441
BtgI CCRYGG 1 cut(s) 2006
BtsCI GGATG 3 cut(s) 334, 2118, 2156
BtsIMutI CAGTG 2 cut(s) 425, 1794
BveI ACCTGC 1 cut(s) 1225
Cac8I GCNNGC 3 cut(s) 73, 1848, 2134
CaiI CAGNNNCTG 1 cut(s) 34
CciI TCATGA 1 cut(s) 1731
CfoI GCGC 8 cut(s) 314, 415, 847, 1020, 1133, 1236, 1288, 2281
Cfr10I RCCGGY 5 cut(s) 506, 675, 768, 1239, 2132
Cfr13I GGNCC 7 cut(s) 250, 387, 733, 1325, 1418, 2049, 2125
ClaI ATCGAT 2 cut(s) 1334, 1441
CpoI CGGWCCG 1 cut(s) 250
CseI GACGC 3 cut(s) 458, 2147, 2189
Csp6I GTAC 3 cut(s) 673, 1453, 1603
CspAI ACCGGT 2 cut(s) 675, 768
CspI CGGWCCG 1 cut(s) 250
CviAII CATG 4 cut(s) 67, 187, 1534, 1732
CviQI GTAC 3 cut(s) 673, 1453, 1603
DdeI CTNAG 3 cut(s) 1484, 1659, 1797
DinI GGCGCC 1 cut(s) 2280
DrdI GACNNNNNNGTC 1 cut(s) 241
DriI GACNNNNNGTC 3 cut(s) 955, 1522, 1714
DseDI GACNNNNNNGTC 1 cut(s) 241
EaeI YGGCCR 3 cut(s) 183, 1134, 1241
EagI CGGCCG 1 cut(s) 1241
Eam1104I CTCTTC 2 cut(s) 354, 1593
Eam1105I GACNNNNNGTC 3 cut(s) 955, 1522, 1714
EarI CTCTTC 2 cut(s) 354, 1593
EciI GGCGGA 4 cut(s) 508, 887, 1526, 1610
Ecl136II GAGCTC 2 cut(s) 516, 1119
EclXI CGGCCG 1 cut(s) 1241
Eco105I TACGTA 1 cut(s) 1831
Eco130I CCWWGG 2 cut(s) 453, 1229
Eco147I AGGCCT 1 cut(s) 1011
Eco24I GRGCYC 2 cut(s) 518, 1121
Eco31I GGTCTC 3 cut(s) 654, 1916, 2023
Eco32I GATATC 1 cut(s) 201
Eco47I GGWCC 6 cut(s) 250, 387, 1325, 1418, 2049, 2125
Eco52I CGGCCG 1 cut(s) 1241
Eco53kI GAGCTC 2 cut(s) 516, 1119
Eco88I CYCGRG 3 cut(s) 469, 884, 1943
EcoICRI GAGCTC 2 cut(s) 516, 1119
EcoNI CCTNNNNNAGG 1 cut(s) 793
EcoO109I RGGNCCY 2 cut(s) 387, 2125
EcoRI GAATTC 1 cut(s) 368
EcoRII CCWGG 3 cut(s) 33, 1022, 1448
EcoRV GATATC 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 453, 1229
EcoT38I GRGCYC 2 cut(s) 518, 1121
EgeI GGCGCC 1 cut(s) 2280
EheI GGCGCC 1 cut(s) 2280
ErhI CCWWGG 2 cut(s) 453, 1229
Esp3I CGTCTC 3 cut(s) 227, 249, 965
FaeI CATG 4 cut(s) 70, 190, 1537, 1735
FalI AAGNNNNNCTT 5 cut(s) 845, 877, 971, 1003, 2282
FaqI GGGAC 2 cut(s) 597, 2090
FatI CATG 4 cut(s) 66, 186, 1533, 1731
FauI CCCGC 2 cut(s) 1839, 2102
FauNDI CATATG 2 cut(s) 1772, 2105
FblI GTMKAC 3 cut(s) 447, 684, 1827
FokI GGATG 3 cut(s) 321, 2125, 2163
FriOI GRGCYC 2 cut(s) 518, 1121
FspBI CTAG 5 cut(s) 573, 1230, 1266, 1892, 1982
GlaI GCGC 8 cut(s) 313, 414, 846, 1019, 1132, 1235, 1287, 2280
GsuI CTGGAG 4 cut(s) 56, 65, 388, 1006
HaeII RGCGCY 2 cut(s) 416, 2282
HaeIII GGCC 5 cut(s) 185, 735, 1011, 1136, 1243
HgaI GACGC 3 cut(s) 458, 2147, 2189
HhaI GCGC 8 cut(s) 314, 415, 847, 1020, 1133, 1236, 1288, 2281
Hin1I GRCGYC 3 cut(s) 450, 2181, 2279
Hin1II CATG 4 cut(s) 70, 190, 1537, 1735
Hin6I GCGC 8 cut(s) 312, 413, 845, 1018, 1131, 1234, 1286, 2279
HinP1I GCGC 8 cut(s) 312, 413, 845, 1018, 1131, 1234, 1286, 2279
HincII GTYRAC 3 cut(s) 448, 685, 1938
HindII GTYRAC 3 cut(s) 448, 685, 1938
HphI GGTGA 7 cut(s) 291, 315, 549, 1118, 1206, 1234, 1963
Hpy166II GTNNAC 8 cut(s) 448, 655, 685, 910, 1126, 1527, 1828, 1938
Hpy8I GTNNAC 8 cut(s) 448, 655, 685, 910, 1126, 1527, 1828, 1938
HpyCH4III ACNGT 9 cut(s) 157, 243, 445, 594, 1090, 1329, 1471, 1969, 1994
HpyCH4IV ACGT 2 cut(s) 974, 1830
HpyCH4V TGCA 9 cut(s) 22, 66, 226, 598, 730, 1000, 1253, 1393, 1666
HpyF10VI GCNNNNNNNGC 7 cut(s) 72, 715, 756, 1017, 1203, 1240, 2138
HpyF3I CTNAG 3 cut(s) 1484, 1659, 1797
HpySE526I ACGT 2 cut(s) 974, 1830
Hsp92I GRCGYC 3 cut(s) 450, 2181, 2279
Hsp92II CATG 4 cut(s) 70, 190, 1537, 1735
HspAI GCGC 8 cut(s) 312, 413, 845, 1018, 1131, 1234, 1286, 2279
KasI GGCGCC 1 cut(s) 2278
Kpn2I TCCGGA 3 cut(s) 247, 610, 627
KroI GCCGGC 1 cut(s) 2132
KroNI GCCGGC 1 cut(s) 2134
Lsp1109I GCAGC 6 cut(s) 610, 811, 1012, 1018, 1357, 1752
LweI GCATC 1 cut(s) 927
MaeI CTAG 5 cut(s) 573, 1230, 1266, 1892, 1982
MaeII ACGT 2 cut(s) 974, 1830
MaeIII GTNAC 4 cut(s) 321, 1566, 1969, 1994
MbiI CCGCTC 1 cut(s) 1545
MflI RGATCY 6 cut(s) 84, 283, 607, 805, 1839, 2239
MhlI GDGCHC 4 cut(s) 518, 776, 1121, 1796
MluCI AATT 7 cut(s) 209, 368, 694, 739, 1045, 1165, 1977
Mly113I GGCGCC 1 cut(s) 2279
MmeI TCCRAC 8 cut(s) 156, 299, 491, 585, 1295, 1605, 1899, 2202
MroI TCCGGA 3 cut(s) 247, 610, 627
MroNI GCCGGC 1 cut(s) 2132
MseI TTAA 3 cut(s) 621, 1044, 1458
MslI CAYNNNNRTG 2 cut(s) 1532, 2171
MspA1I CMGCKG 1 cut(s) 1348
MspR9I CCNGG 4 cut(s) 35, 1024, 1450, 2069
Mva1269I GAATGC 2 cut(s) 1255, 1393
MvaI CCWGG 3 cut(s) 35, 1024, 1450
MvnI CGCG 7 cut(s) 312, 1131, 1133, 1236, 1286, 1288, 2141
MwoI GCNNNNNNNGC 7 cut(s) 72, 715, 756, 1017, 1203, 1240, 2138
NaeI GCCGGC 1 cut(s) 2134
NarI GGCGCC 1 cut(s) 2279
NciI CCSGG 1 cut(s) 2069
NdeI CATATG 2 cut(s) 1772, 2105
NgoMIV GCCGGC 1 cut(s) 2132
NlaIII CATG 4 cut(s) 70, 190, 1537, 1735
NlaIV GGNNCC 8 cut(s) 240, 330, 609, 615, 787, 807, 1259, 2280
NmeAIII GCCGAG 4 cut(s) 128, 441, 848, 1140
NmuCI GTSAC 4 cut(s) 321, 1566, 1969, 1994
NspV TTCGAA 3 cut(s) 366, 481, 990
PaeR7I CTCGAG 1 cut(s) 1943
PagI TCATGA 1 cut(s) 1731
PaqCI CACCTGC 1 cut(s) 1225
PceI AGGCCT 1 cut(s) 1011
PcsI WCGNNNNNNNCGW 4 cut(s) 161, 389, 531, 2175
PctI GAATGC 2 cut(s) 1255, 1393
PdiI GCCGGC 1 cut(s) 2134
PfeI GAWTC 9 cut(s) 478, 644, 945, 1361, 1438, 1520, 1712, 1805, 2064
PflFI GACNNNGTC 1 cut(s) 1967
PfoI TCCNGGA 2 cut(s) 1022, 2067
PinAI ACCGGT 2 cut(s) 675, 768
Ple19I CGATCG 4 cut(s) 94, 257, 1069, 1338
PluTI GGCGCC 1 cut(s) 2282
Ppu21I YACGTR 1 cut(s) 1831
PpuMI RGGWCCY 2 cut(s) 387, 2125
PshBI ATTAAT 1 cut(s) 1044
Psp124BI GAGCTC 2 cut(s) 518, 1121
Psp5II RGGWCCY 2 cut(s) 387, 2125
Psp6I CCWGG 3 cut(s) 33, 1022, 1448
PspGI CCWGG 3 cut(s) 33, 1022, 1448
PspN4I GGNNCC 8 cut(s) 240, 330, 609, 615, 787, 807, 1259, 2280
PspPI GGNCC 7 cut(s) 250, 387, 733, 1325, 1418, 2049, 2125
PspPPI RGGWCCY 2 cut(s) 387, 2125
PstI CTGCAG 2 cut(s) 228, 1002
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 6 cut(s) 84, 283, 607, 805, 1839, 2239
PsyI GACNNNGTC 1 cut(s) 1967
PvuI CGATCG 4 cut(s) 94, 257, 1069, 1338
RsaI GTAC 3 cut(s) 674, 1454, 1604
RsaNI GTAC 3 cut(s) 673, 1453, 1603
RseI CAYNNNNRTG 2 cut(s) 1532, 2171
Rsr2I CGGWCCG 1 cut(s) 250
RsrII CGGWCCG 1 cut(s) 250
SacI GAGCTC 2 cut(s) 518, 1121
SalI GTCGAC 2 cut(s) 446, 683
SaqAI TTAA 3 cut(s) 621, 1044, 1458
Sau96I GGNCC 7 cut(s) 250, 387, 733, 1325, 1418, 2049, 2125
ScrFI CCNGG 4 cut(s) 35, 1024, 1450, 2069
SduI GDGCHC 4 cut(s) 518, 776, 1121, 1796
SfaNI GCATC 1 cut(s) 927
SfcI CTRYAG 2 cut(s) 224, 998
SfoI GGCGCC 1 cut(s) 2280
Sfr274I CTCGAG 1 cut(s) 1943
SfuI TTCGAA 3 cut(s) 366, 481, 990
SgrDI CGTCGACG 2 cut(s) 446, 683
SinI GGWCC 6 cut(s) 250, 387, 1325, 1418, 2049, 2125
SlaI CTCGAG 1 cut(s) 1943
SmiMI CAYNNNNRTG 2 cut(s) 1532, 2171
SmlI CTYRAG 1 cut(s) 1943
SmoI CTYRAG 1 cut(s) 1943
SnaBI TACGTA 1 cut(s) 1831
Sse9I AATT 7 cut(s) 209, 368, 694, 739, 1045, 1165, 1977
SseBI AGGCCT 1 cut(s) 1011
SspDI GGCGCC 1 cut(s) 2278
SspMI CTAG 5 cut(s) 573, 1230, 1266, 1892, 1982
SstI GAGCTC 2 cut(s) 518, 1121
StuI AGGCCT 1 cut(s) 1011
StyD4I CCNGG 4 cut(s) 33, 1022, 1448, 2067
StyI CCWWGG 2 cut(s) 453, 1229
TaaI ACNGT 9 cut(s) 157, 243, 445, 594, 1090, 1329, 1471, 1969, 1994
TaiI ACGT 2 cut(s) 977, 1833
TaqII GACCGA 2 cut(s) 267, 2001
TasI AATT 7 cut(s) 209, 368, 694, 739, 1045, 1165, 1977
TatI WGTACW 1 cut(s) 1602
TauI GCSGC 9 cut(s) 718, 1136, 1159, 1162, 1286, 1299, 1548, 2005, 2249
TfiI GAWTC 9 cut(s) 478, 644, 945, 1361, 1438, 1520, 1712, 1805, 2064
Tru1I TTAA 3 cut(s) 621, 1044, 1458
Tru9I TTAA 3 cut(s) 621, 1044, 1458
TscAI CASTG 2 cut(s) 432, 1801
TseFI GTSAC 4 cut(s) 321, 1566, 1969, 1994
TseI GCWGC 6 cut(s) 598, 799, 1000, 1031, 1345, 1740
Tsp45I GTSAC 4 cut(s) 321, 1566, 1969, 1994
TspDTI ATGAA 2 cut(s) 1112, 1748
TspGWI ACGGA 5 cut(s) 126, 540, 895, 1270, 2163
TspRI CASTG 2 cut(s) 432, 1801
Tth111I GACNNNGTC 1 cut(s) 1967
VpaK11BI GGWCC 6 cut(s) 250, 387, 1325, 1418, 2049, 2125
VspI ATTAAT 1 cut(s) 1044
XagI CCTNNNNNAGG 1 cut(s) 793
XapI RAATTY 3 cut(s) 368, 1165, 1977
XbaI TCTAGA 1 cut(s) 1981
XhoI CTCGAG 1 cut(s) 1943
XmaJI CCTAGG 1 cut(s) 1229
XmiI GTMKAC 3 cut(s) 447, 684, 1827
XspI CTAG 5 cut(s) 573, 1230, 1266, 1892, 1982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.