pycom03g20300

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008556
Physical Location & Seq
Reverse (-)
20827920 .. 20828462
543 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20300.1

Sequence Viewer

Length: 543 bp
ATGGAAGACATTTGGGGGGAGGCAATGTGGGGAGCCGAGTCCGAAATCGAAGACGGGGATTTCATCTATGTATCGGAGATCCTTCGGGTTTCCAATTACTTACCGGAAGACTCCGACATTTTCCTATTACTAGAGAAGAAGCATCATTTCAAGGGAAAGGACACCTCCAAAGAGTCAACACTCGAGAGAAAGCTCATTTTCGACACCGTCACCGAAATTCTTGACCGAAACCGCCGATTGCCGGCATGGAAGGCAGTCTCGTGGCAAACTTCATTGCCCGATATTTGGTCGGAGTTTCGGAGAATCCGAGAGAGGGACGAGTCCGAGGACTTGTTCGAGGTCATATGCGGGGTGTTGAAAAAGGACCTTGCAGGCGACTCAATCAACGGTTGGGGAGATTGTTGGATCGAAATGTCCGACGCCGTTTTGGACATCGAACGGCTCATTTTCAAGGACTTGATCGGCGAAACGATCGGAGATCTCGCTGCTTTTCCCACCAACTATAGTAAAGTTTCGGCGCCTTGTAGGAAGTTGGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

181

Amino Acids

20.84

Weight (kDa)

4.59

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 20 - 156 5.7e-16 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 517
AciI CCGC 2 cut(s) 232, 348
AclWI GGATC 2 cut(s) 73, 413
AcsI RAATTY 1 cut(s) 216
AcyI GRCGYC 2 cut(s) 420, 518
AfiI CCNNNNNNNGG 3 cut(s) 241, 285, 313
AgsI TTSAA 3 cut(s) 151, 358, 451
AluBI AGCT 1 cut(s) 193
AluI AGCT 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 2 cut(s) 73, 413
Ama87I CYCGRG 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 1 cut(s) 216
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 364
AsuHPI GGTGA 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 517
BauI CACGAG 1 cut(s) 259
BbsI GAAGAC 4 cut(s) 12, 57, 114, 529
BbvI GCAGC 1 cut(s) 472
BceAI ACGGC 2 cut(s) 407, 455
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 1 cut(s) 131
BfmI CTRYAG 1 cut(s) 502
BfoI RGCGCY 1 cut(s) 521
BglII AGATCT 1 cut(s) 478
BisI GCNGC 1 cut(s) 486
BlsI GCNGC 1 cut(s) 487
Bme18I GGWCC 1 cut(s) 364
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 2 cut(s) 34, 519
BmsI GCATC 1 cut(s) 151
BpiI GAAGAC 4 cut(s) 12, 57, 114, 529
BsaHI GRCGYC 2 cut(s) 420, 518
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 285, 313
Bse118I RCCGGY 1 cut(s) 241
Bse3DI GCAATG 2 cut(s) 30, 272
BseDI CCNNGG 1 cut(s) 324
BseLI CCNNNNNNNGG 3 cut(s) 241, 285, 313
BseMI GCAATG 2 cut(s) 30, 272
BseXI GCAGC 1 cut(s) 472
Bsh1285I CGRYCG 1 cut(s) 474
BshNI GGYRCC 1 cut(s) 517
BsiEI CGRYCG 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 2 cut(s) 104, 242
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 3 cut(s) 241, 285, 313
BsmAI GTCTC 1 cut(s) 262
BsmFI GGGAC 1 cut(s) 329
BsoBI CYCGRG 1 cut(s) 182
Bsp143I GATC 5 cut(s) 78, 405, 459, 471, 478
BspACI CCGC 2 cut(s) 232, 348
BspLI GGNNCC 2 cut(s) 34, 519
BspPI GGATC 2 cut(s) 73, 413
BspT107I GGYRCC 1 cut(s) 517
BsrDI GCAATG 2 cut(s) 30, 272
BsrFI RCCGGY 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 5 cut(s) 78, 405, 459, 471, 478
BssNI GRCGYC 2 cut(s) 420, 518
BssSI CACGAG 1 cut(s) 259
Bst2BI CACGAG 1 cut(s) 259
Bst4CI ACNGT 2 cut(s) 208, 389
BstACI GRCGYC 2 cut(s) 420, 518
BstC8I GCNNGC 2 cut(s) 243, 373
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 5 cut(s) 81, 408, 462, 474, 481
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 5 cut(s) 78, 405, 459, 471, 478
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 502
BstV1I GCAGC 1 cut(s) 472
BstV2I GAAGAC 4 cut(s) 12, 57, 114, 529
BstX2I RGATCY 2 cut(s) 78, 478
BstYI RGATCY 2 cut(s) 78, 478
Cac8I GCNNGC 2 cut(s) 243, 373
CfoI GCGC 1 cut(s) 520
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 364
CseI GACGC 1 cut(s) 428
CviAII CATG 1 cut(s) 246
CviJI RGCY 3 cut(s) 35, 193, 442
CviKI_1 RGCY 3 cut(s) 35, 193, 442
DinI GGCGCC 1 cut(s) 519
DpnI GATC 5 cut(s) 80, 407, 461, 473, 480
DpnII GATC 5 cut(s) 78, 405, 459, 471, 478
Eco47I GGWCC 1 cut(s) 364
Eco88I CYCGRG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 364
EgeI GGCGCC 1 cut(s) 519
EheI GGCGCC 1 cut(s) 519
FaeI CATG 1 cut(s) 249
FaiI YATR 5 cut(s) 69, 247, 344, 346, 504
FalI AAGNNNNNCTT 1 cut(s) 521
FaqI GGGAC 1 cut(s) 329
FatI CATG 1 cut(s) 245
FauI CCCGC 1 cut(s) 341
FauNDI CATATG 1 cut(s) 344
Fnu4HI GCNGC 1 cut(s) 486
Fsp4HI GCNGC 1 cut(s) 486
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 519
GluI GCNGC 1 cut(s) 486
HaeII RGCGCY 1 cut(s) 521
HapII CCGG 2 cut(s) 104, 242
HgaI GACGC 1 cut(s) 428
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 2 cut(s) 420, 518
Hin1II CATG 1 cut(s) 249
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HinfI GANTC 6 cut(s) 38, 110, 173, 303, 320, 377
HpaII CCGG 2 cut(s) 104, 242
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 9 cut(s) 43, 76, 115, 292, 300, 308, 325, 418, 476
Hpy188III TCNNGA 2 cut(s) 184, 221
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 2 cut(s) 92, 244
HpyCH4III ACNGT 2 cut(s) 208, 389
HpyCH4V TGCA 1 cut(s) 371
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
Hsp92I GRCGYC 2 cut(s) 420, 518
Hsp92II CATG 1 cut(s) 249
HspAI GCGC 1 cut(s) 518
KasI GGCGCC 1 cut(s) 517
KroI GCCGGC 1 cut(s) 241
KroNI GCCGGC 1 cut(s) 243
Kzo9I GATC 5 cut(s) 78, 405, 459, 471, 478
LmnI GCTCC 1 cut(s) 32
LpnPI CCDG 3 cut(s) 117, 255, 357
Lsp1109I GCAGC 1 cut(s) 472
LweI GCATC 1 cut(s) 151
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 1 cut(s) 208
MalI GATC 5 cut(s) 80, 407, 461, 473, 480
MboI GATC 5 cut(s) 78, 405, 459, 471, 478
MboII GAAGA 5 cut(s) 17, 62, 119, 148, 529
MflI RGATCY 2 cut(s) 78, 478
MluCI AATT 2 cut(s) 94, 216
Mly113I GGCGCC 1 cut(s) 518
MlyI GAGTC 5 cut(s) 47, 104, 182, 329, 371
MmeI TCCRAC 4 cut(s) 138, 270, 383, 441
MnlI CCTC 5 cut(s) 13, 175, 306, 319, 331
MroNI GCCGGC 1 cut(s) 241
MspI CCGG 2 cut(s) 104, 242
MwoI GCNNNNNNNGC 1 cut(s) 251
NaeI GCCGGC 1 cut(s) 243
NarI GGCGCC 1 cut(s) 518
NdeI CATATG 1 cut(s) 344
NdeII GATC 5 cut(s) 78, 405, 459, 471, 478
NgoMIV GCCGGC 1 cut(s) 241
NlaIII CATG 1 cut(s) 249
NlaIV GGNNCC 2 cut(s) 34, 519
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 208
PaeR7I CTCGAG 1 cut(s) 182
PcsI WCGNNNNNNNCGW 2 cut(s) 304, 414
PdiI GCCGGC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 303
PflFI GACNNNGTC 1 cut(s) 206
PkrI GCNGC 1 cut(s) 487
Ple19I CGATCG 1 cut(s) 474
PleI GAGTC 5 cut(s) 46, 104, 181, 328, 371
PluTI GGCGCC 1 cut(s) 521
PpsI GAGTC 5 cut(s) 46, 104, 181, 328, 371
PpuMI RGGWCCY 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 364
PspN4I GGNNCC 2 cut(s) 34, 519
PspPI GGNCC 1 cut(s) 364
PspPPI RGGWCCY 1 cut(s) 364
PsuI RGATCY 2 cut(s) 78, 478
PsyI GACNNNGTC 1 cut(s) 206
PvuI CGATCG 1 cut(s) 474
SatI GCNGC 1 cut(s) 486
Sau3AI GATC 5 cut(s) 78, 405, 459, 471, 478
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 5 cut(s) 47, 104, 182, 329, 371
SetI ASST 4 cut(s) 167, 195, 342, 369
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 1 cut(s) 502
SfoI GGCGCC 1 cut(s) 519
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 1 cut(s) 364
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 2 cut(s) 94, 216
SsiI CCGC 2 cut(s) 232, 348
SspDI GGCGCC 1 cut(s) 517
SspMI CTAG 1 cut(s) 131
TaaI ACNGT 2 cut(s) 208, 389
TaqI TCGA 6 cut(s) 48, 183, 201, 336, 408, 435
TaqII GACCGA 1 cut(s) 240
TasI AATT 2 cut(s) 94, 216
TfiI GAWTC 1 cut(s) 303
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 2 cut(s) 52, 261
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 364
XapI RAATTY 1 cut(s) 216
XhoI CTCGAG 1 cut(s) 182
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.