MD03G1256300.v1.1

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008556
Physical Location & Seq
Reverse (-)
34381364 .. 34385235
3872 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1256300.v1.1.491

Sequence Viewer

Length: 2289 bp
ATGACGACTGGAAGAATGGTGCAAGAACAGAACCTGGAGAAGCACATAAAGAAACAGATGGGGTGCATGGCTGGCTTCCTCCATATCTTCGATCGCCACCAGCTTCTCAACGCCAAACGCCTCCCTTCCTCGGCGACGGTGGTGTCGTCGCCAGAGTCGGAGTCGGCCACATCGCCGGAGATATCCAAAGAATTGGAAAAACAACCGCAGAACAAGACGGCACCGTCTCCAGACCGATCGAAGCAATCTCCATCTCCGATGACGGATCTCCGACCTCCGTCACCGGAAACTCGCGCCCCTTCGTCGCCGGAACCTTATCCGAAATCTCCTCTTCCGCTTCCAGTTTTCGAGTTCAAAGAAGGCACGAGGTCGTCGTGGAAGTTTTCCAGAGAAGCCCCGAGGCTCTCACTCGATAGCAGAGCCACCGTCGACGCCAAAGGAAGCCTATACCCGAGGGAGATCCGAACAAACGCCGCCATACTAACTGCGAGTCGAAGCTCCAACGTCGACGGAGGAAGTGATAGAGATAAGCAACACCGATCGCCCAGCGTTATTGCGAGGCTAATGGGACTTGAACCAATACAGCAGTCAAATCCTGAACCGATTAAGCTCCGGAGATCCGCGTCCGAGTCCAGAGTGAACCGAGATCTGAACCCGTACCGGTTCGTCGACGGAAACAATTTCCAGCAGAAGCAACCGCCACAACAGAATGTGCAGAGCCATAATTTTTCCAGCAATGCAGGCAAAGGCGACCGGAGCTCCAATCGGGGCACCAGTCCGAGGCAGCCAGATCCGAAGAACAATGCGAAGGCTCAGAGCAAAGGAATGGCGCAGAGGAAGTGCTTCTACGACTCGGCGGACTTCTTCCCGGAGCCGAAGCAGAGCGTCTCTGTATACAACGAGATTGAGAAGAGGTTGAAGATGCGAGGAATCGACGAGCCGTCGAAAGATTTGGAGACGTTGAAGCAAATCCTCGAAGCTCTGCAGCTCAAAGGCCTCCTGCACTCCAGGAAAGCTTCCAATCAGATTAATTCCAGAAACTTCGTCTACGATCGGAGCTTCGAATCGCCGATTGTTGTAATGAAGCCGTCCAGATCGCCGAGCTCGACTCACCGTGCGGCCAGATTCGGAAACGAGTCGCCGCCGCCGAGTTCGGGGATCAGGTCTAGGGCAGGAGTTCGCCGGAACGGCGAGGTGTCGCCTGCGGTGAGCCCGAGGCGCAACCTGCCGGAGAATGTACGGAGCCAGACCAGAGGGAGGAGCCCGGCAGCGCGCGAGGGCGGCGTAAGGAGTCCTAGTAGGAGAGGAGGACCGTCATCGATCGAAACGCAGCGGAAGGGGAATGATTCGGTGGAGCACAGGAGAGTGTCTCCAGTGCAGTCGCCTAAAATCAGTTCAAGAAGACCAGACCAAACGGCGGCGAATCGATCTCCCAGGAACATCAAGAAACCAACGGTTGAGATTGCTCCCAGGGAGGAAAAGGTATTTCATCGGGCGGAGGACGAATATTCCACCACCATGTCAGAGAGCAGCATTAGCAATAGCACCTCTTCACAAGCCGACACGGAGAGGTGGAAGGCGGAGGAGTACAAAGAACGGAGGAGCTCGTTGGAGAGGTGCGATAAGCTGCTTCACAGCATAGCTGAGTTGCAACCGAGTCCTGTCTCGGTGCTCGACTCCTCGTTTTACAAAGAGGAGTCTTCGCCTTCGCCGGTCATGAAACGCAGCATTGATTTCAAAGTTGAAATGGAAGACATTTGGGGGGAGGCAACGTGGGGAGCCGAGTCCGAAATCGAAGACGGGGATTTCGTCTATGTATCGGAGATCCTTCGGGCTTCCAATTACTTGCCGGAAGACTCCGACATTTTCCTATTACTAGAGAAGAAGCAACATTTCAAGGGAAAGGACACCTCCAAAGAGTCAACACTCGAGAGAAGGCTCATTTTCGACACCGTCATCGAAATTCTCGACCGAAACCGCCGATTGCCTCCGTGGAAGGCGGTCTCGTGGCAGACTTCGTTGCCCGAAATTTGGTCGGAGTTTCGGAGAATCCGGGAGAGGGACGAGTCCGAGGAATTGTTCGAGGTCATATGCGGGGTGTTGAAAAAGGACCTTGCAGGCGACTCAATCAACGGATGGGGAGATTGTTCGATCGAAATGTCCGACGCCGTTTTGGACATCGAACGGCTCATTTTCAAGGACTTGATCGGCGAAACGATCCGAGATCTCGCTGCTTTTCCCTCCAACTATAGTAAAGTTTCGGCGCCTTGTAGGAAGTTGGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

763

Amino Acids

85.95

Weight (kDa)

9.4

Isoelectric Point (pI)

85.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 602 - 738 5.4e-17 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 142, 223
Acc36I ACCTGC 1 cut(s) 1233
AccB1I GGYRCC 3 cut(s) 220, 770, 2263
AccI GTMKAC 5 cut(s) 429, 507, 669, 894, 1047
AccII CGCG 4 cut(s) 294, 623, 1273, 1275
AccIII TCCGGA 1 cut(s) 612
AclWI GGATC 7 cut(s) 273, 454, 612, 785, 1166, 1819, 2212
AcoI YGGCCR 2 cut(s) 165, 1119
AcsI RAATTY 2 cut(s) 1962, 2028
AcyI GRCGYC 3 cut(s) 432, 2166, 2264
AfaI GTAC 3 cut(s) 659, 1239, 1589
AfiI CCNNNNNNNGG 7 cut(s) 130, 780, 1154, 1257, 1417, 2031, 2059
AgeI ACCGGT 1 cut(s) 660
AhdI GACNNNNNGTC 1 cut(s) 940
AjnI CCWGG 4 cut(s) 33, 1007, 1433, 1469
Alw21I GWGCWC 5 cut(s) 761, 1106, 1359, 1607, 1674
Alw26I GTCTC 6 cut(s) 231, 892, 950, 1374, 1669, 2008
AlwI GGATC 7 cut(s) 273, 454, 612, 785, 1166, 1819, 2212
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 4 cut(s) 397, 451, 1213, 1928
Aor13HI TCCGGA 1 cut(s) 612
AoxI GGCC 3 cut(s) 165, 994, 1119
ApeKI GCWGC 8 cut(s) 784, 985, 1268, 1330, 1530, 1627, 1725, 2231
ApoI RAATTY 2 cut(s) 1962, 2028
ArsI GACNNNNNNTTYG 2 cut(s) 1790, 1822
AseI ATTAAT 1 cut(s) 1029
AsiGI ACCGGT 1 cut(s) 660
Asp700I GAANNNNTTC 1 cut(s) 842
AspLEI GCGC 6 cut(s) 296, 832, 1221, 1273, 1275, 2266
AspS9I GGNCC 2 cut(s) 1310, 2110
AsuC2I CCSGG 3 cut(s) 869, 1265, 2054
AsuHPI GGTGA 3 cut(s) 273, 1103, 1219
AsuII TTCGAA 1 cut(s) 1062
AvaI CYCGRG 4 cut(s) 397, 451, 1213, 1928
AvaII GGWCC 2 cut(s) 1310, 2110
BaeGI GKGCMC 1 cut(s) 773
BanI GGYRCC 3 cut(s) 220, 770, 2263
BanII GRGCYC 5 cut(s) 761, 1106, 1214, 1265, 1607
BarI GAAGNNNNNNTAC 2 cut(s) 830, 862
BauI CACGAG 2 cut(s) 364, 2005
BbsI GAAGAC 6 cut(s) 1408, 1692, 1758, 1803, 1860, 2275
Bbv12I GWGCWC 5 cut(s) 761, 1106, 1359, 1607, 1674
BbvI GCAGC 8 cut(s) 796, 997, 1280, 1342, 1542, 1614, 1737, 2218
BccI CCATC 3 cut(s) 52, 259, 2130
BceAI ACGGC 7 cut(s) 234, 925, 1072, 1204, 1431, 2153, 2201
BciT130I CCWGG 4 cut(s) 35, 1009, 1435, 1471
BcnI CCSGG 3 cut(s) 869, 1265, 2054
BcoDI GTCTC 6 cut(s) 231, 892, 950, 1374, 1669, 2008
BfaI CTAG 3 cut(s) 1167, 1296, 1877
BfmI CTRYAG 2 cut(s) 983, 2248
BfoI RGCGCY 1 cut(s) 2267
BfuAI ACCTGC 1 cut(s) 1233
BglI GCCNNNNNGGC 1 cut(s) 1188
BglII AGATCT 2 cut(s) 646, 2224
Bme1390I CCNGG 7 cut(s) 35, 869, 1009, 1265, 1435, 1471, 2054
Bme18I GGWCC 2 cut(s) 1310, 2110
BmeRI GACNNNNNGTC 1 cut(s) 940
BmeT110I CYCGRG 4 cut(s) 397, 451, 1213, 1928
BmgT120I GGNCC 2 cut(s) 1310, 2110
BmiI GGNNCC 8 cut(s) 222, 312, 772, 873, 1244, 1262, 1780, 2265
BmrFI CCNGG 7 cut(s) 35, 869, 1009, 1265, 1435, 1471, 2054
BmsI GCATC 1 cut(s) 912
BoxI GACNNNNGTC 1 cut(s) 277
BpiI GAAGAC 6 cut(s) 1408, 1692, 1758, 1803, 1860, 2275
BplI GAGNNNNNCTC 4 cut(s) 1093, 1125, 1354, 1386
BpmI CTGGAG 4 cut(s) 56, 213, 991, 1356
Bpu14I TTCGAA 1 cut(s) 1062
BpuMI CCSGG 3 cut(s) 869, 1265, 2054
Bsa29I ATCGAT 2 cut(s) 1319, 1426
BsaHI GRCGYC 3 cut(s) 432, 2166, 2264
BsaI GGTCTC 1 cut(s) 2008
BsaWI WCCGGW 4 cut(s) 283, 612, 660, 753
BsaXI ACNNNNNCTCC 6 cut(s) 743, 773, 1282, 1312, 1757, 1787
Bsc4I CCNNNNNNNGG 7 cut(s) 130, 780, 1154, 1257, 1417, 2031, 2059
Bse118I RCCGGY 2 cut(s) 660, 1711
Bse1I ACTGG 4 cut(s) 13, 341, 774, 1373
Bse3DI GCAATG 1 cut(s) 742
BseAI TCCGGA 1 cut(s) 612
BseBI CCWGG 4 cut(s) 35, 1009, 1435, 1471
BseCI ATCGAT 2 cut(s) 1319, 1426
BseGI GGATG 1 cut(s) 2141
BseLI CCNNNNNNNGG 7 cut(s) 130, 780, 1154, 1257, 1417, 2031, 2059
BseMI GCAATG 1 cut(s) 742
BseMII CTCAG 2 cut(s) 827, 1635
BseNI ACTGG 4 cut(s) 13, 341, 774, 1373
BsePI GCGCGC 1 cut(s) 1271
BseRI GAGGAG 7 cut(s) 318, 1273, 1320, 1598, 1615, 1669, 1709
BseSI GKGCMC 1 cut(s) 773
BseXI GCAGC 8 cut(s) 796, 997, 1280, 1342, 1542, 1614, 1737, 2218
BseYI CCCAGC 1 cut(s) 545
BsgI GTGCAG 3 cut(s) 734, 986, 1397
Bsh1236I CGCG 4 cut(s) 294, 623, 1273, 1275
Bsh1285I CGRYCG 8 cut(s) 94, 239, 542, 754, 1054, 1323, 1972, 2154
BshFI GGCC 3 cut(s) 167, 996, 1121
BshNI GGYRCC 3 cut(s) 220, 770, 2263
BshTI ACCGGT 1 cut(s) 660
BshVI ATCGAT 2 cut(s) 1319, 1426
BsiEI CGRYCG 8 cut(s) 94, 239, 542, 754, 1054, 1323, 1972, 2154
BsiHKAI GWGCWC 5 cut(s) 761, 1106, 1359, 1607, 1674
BsiHKCI CYCGRG 4 cut(s) 397, 451, 1213, 1928
BslFI GGGAC 2 cut(s) 582, 2075
BslI CCNNNNNNNGG 7 cut(s) 130, 780, 1154, 1257, 1417, 2031, 2059
BsmAI GTCTC 6 cut(s) 231, 892, 950, 1374, 1669, 2008
BsmBI CGTCTC 3 cut(s) 231, 892, 950
BsmFI GGGAC 2 cut(s) 582, 2075
BsnI GGCC 3 cut(s) 167, 996, 1121
Bso31I GGTCTC 1 cut(s) 2008
BsoBI CYCGRG 4 cut(s) 397, 451, 1213, 1928
Bsp119I TTCGAA 1 cut(s) 1062
Bsp1286I GDGCHC 8 cut(s) 761, 773, 1106, 1214, 1265, 1359, 1607, 1674
Bsp13I TCCGGA 1 cut(s) 612
BspANI GGCC 3 cut(s) 167, 996, 1121
BspCNI CTCAG 2 cut(s) 826, 1636
BspDI ATCGAT 2 cut(s) 1319, 1426
BspEI TCCGGA 1 cut(s) 612
BspFNI CGCG 4 cut(s) 294, 623, 1273, 1275
BspHI TCATGA 1 cut(s) 1716
BspLI GGNNCC 8 cut(s) 222, 312, 772, 873, 1244, 1262, 1780, 2265
BspMAI CTGCAG 1 cut(s) 987
BspMI ACCTGC 1 cut(s) 1233
BspPI GGATC 7 cut(s) 273, 454, 612, 785, 1166, 1819, 2212
BspT104I TTCGAA 1 cut(s) 1062
BspT107I GGYRCC 3 cut(s) 220, 770, 2263
BspTNI GGTCTC 1 cut(s) 2008
BsrDI GCAATG 1 cut(s) 742
BsrFI RCCGGY 2 cut(s) 660, 1711
BsrI ACTGG 4 cut(s) 13, 341, 774, 1373
BssAI RCCGGY 2 cut(s) 660, 1711
BssHII GCGCGC 1 cut(s) 1271
BssNAI GTATAC 1 cut(s) 895
BssNI GRCGYC 3 cut(s) 432, 2166, 2264
BssSI CACGAG 2 cut(s) 364, 2005
Bst1107I GTATAC 1 cut(s) 895
Bst2BI CACGAG 2 cut(s) 364, 2005
Bst2UI CCWGG 4 cut(s) 35, 1009, 1435, 1471
Bst4CI ACNGT 7 cut(s) 139, 225, 427, 1115, 1314, 1456, 1954
Bst6I CTCTTC 3 cut(s) 336, 905, 1555
BstACI GRCGYC 3 cut(s) 432, 2166, 2264
BstBI TTCGAA 1 cut(s) 1062
BstC8I GCNNGC 5 cut(s) 73, 742, 1203, 1273, 2119
BstDEI CTNAG 2 cut(s) 813, 1644
BstDSI CCRYGG 1 cut(s) 1991
BstF5I GGATG 1 cut(s) 2141
BstFNI CGCG 4 cut(s) 294, 623, 1273, 1275
BstH2I RGCGCY 1 cut(s) 2267
BstHHI GCGC 6 cut(s) 296, 832, 1221, 1273, 1275, 2266
BstMAI GTCTC 6 cut(s) 231, 892, 950, 1374, 1669, 2008
BstMCI CGRYCG 8 cut(s) 94, 239, 542, 754, 1054, 1323, 1972, 2154
BstMWI GCNNNNNNNGC 8 cut(s) 72, 741, 756, 1188, 1218, 1225, 1281, 1536
BstNI CCWGG 4 cut(s) 35, 1009, 1435, 1471
BstPAI GACNNNNGTC 1 cut(s) 277
BstSCI CCNGG 7 cut(s) 33, 867, 1007, 1263, 1433, 1469, 2052
BstSFI CTRYAG 2 cut(s) 983, 2248
BstSLI GKGCMC 1 cut(s) 773
BstUI CGCG 4 cut(s) 294, 623, 1273, 1275
BstV1I GCAGC 8 cut(s) 796, 997, 1280, 1342, 1542, 1614, 1737, 2218
BstV2I GAAGAC 6 cut(s) 1408, 1692, 1758, 1803, 1860, 2275
BstX2I RGATCY 7 cut(s) 265, 459, 617, 646, 790, 1824, 2224
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 7 cut(s) 265, 459, 617, 646, 790, 1824, 2224
BstZ17I GTATAC 1 cut(s) 895
Bsu15I ATCGAT 2 cut(s) 1319, 1426
BsuRI GGCC 3 cut(s) 167, 996, 1121
BsuTUI ATCGAT 2 cut(s) 1319, 1426
BtgI CCRYGG 1 cut(s) 1991
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 1 cut(s) 2141
BtsIMutI CAGTG 1 cut(s) 1380
BveI ACCTGC 1 cut(s) 1233
Cac8I GCNNGC 5 cut(s) 73, 742, 1203, 1273, 2119
CaiI CAGNNNCTG 1 cut(s) 34
CciI TCATGA 1 cut(s) 1716
CfoI GCGC 6 cut(s) 296, 832, 1221, 1273, 1275, 2266
Cfr10I RCCGGY 2 cut(s) 660, 1711
Cfr13I GGNCC 2 cut(s) 1310, 2110
ClaI ATCGAT 2 cut(s) 1319, 1426
CseI GACGC 4 cut(s) 440, 612, 874, 2174
Csp6I GTAC 3 cut(s) 658, 1238, 1588
CspAI ACCGGT 1 cut(s) 660
CviAII CATG 3 cut(s) 67, 1519, 1717
CviQI GTAC 3 cut(s) 658, 1238, 1588
DdeI CTNAG 2 cut(s) 813, 1644
DinI GGCGCC 1 cut(s) 2265
DrdI GACNNNNNNGTC 2 cut(s) 142, 223
DriI GACNNNNNGTC 1 cut(s) 940
DseDI GACNNNNNNGTC 2 cut(s) 142, 223
EaeI YGGCCR 2 cut(s) 165, 1119
Eam1104I CTCTTC 3 cut(s) 336, 905, 1555
Eam1105I GACNNNNNGTC 1 cut(s) 940
EarI CTCTTC 3 cut(s) 336, 905, 1555
EciI GGCGGA 3 cut(s) 872, 1511, 1595
Ecl136II GAGCTC 3 cut(s) 759, 1104, 1605
Eco147I AGGCCT 1 cut(s) 996
Eco24I GRGCYC 5 cut(s) 761, 1106, 1214, 1265, 1607
Eco31I GGTCTC 1 cut(s) 2008
Eco32I GATATC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 1310, 2110
Eco53kI GAGCTC 3 cut(s) 759, 1104, 1605
Eco88I CYCGRG 4 cut(s) 397, 451, 1213, 1928
EcoICRI GAGCTC 3 cut(s) 759, 1104, 1605
EcoO109I RGGNCCY 1 cut(s) 2110
EcoRII CCWGG 4 cut(s) 33, 1007, 1433, 1469
EcoRV GATATC 1 cut(s) 183
EcoT38I GRGCYC 5 cut(s) 761, 1106, 1214, 1265, 1607
EgeI GGCGCC 1 cut(s) 2265
EheI GGCGCC 1 cut(s) 2265
Esp3I CGTCTC 3 cut(s) 231, 892, 950
FaeI CATG 3 cut(s) 70, 1522, 1720
FalI AAGNNNNNCTT 1 cut(s) 2267
FaqI GGGAC 2 cut(s) 582, 2075
FatI CATG 3 cut(s) 66, 1518, 1716
FauI CCCGC 1 cut(s) 2087
FauNDI CATATG 1 cut(s) 2090
FblI GTMKAC 5 cut(s) 429, 507, 669, 894, 1047
FokI GGATG 1 cut(s) 2148
FriOI GRGCYC 5 cut(s) 761, 1106, 1214, 1265, 1607
FspBI CTAG 3 cut(s) 1167, 1296, 1877
GlaI GCGC 6 cut(s) 295, 831, 1220, 1272, 1274, 2265
GsaI CCCAGC 1 cut(s) 549
GsuI CTGGAG 4 cut(s) 56, 213, 991, 1356
HaeII RGCGCY 1 cut(s) 2267
HaeIII GGCC 3 cut(s) 167, 996, 1121
HgaI GACGC 4 cut(s) 440, 612, 874, 2174
HhaI GCGC 6 cut(s) 296, 832, 1221, 1273, 1275, 2266
Hin1I GRCGYC 3 cut(s) 432, 2166, 2264
Hin1II CATG 3 cut(s) 70, 1522, 1720
Hin6I GCGC 6 cut(s) 294, 830, 1219, 1271, 1273, 2264
HinP1I GCGC 6 cut(s) 294, 830, 1219, 1271, 1273, 2264
HincII GTYRAC 4 cut(s) 430, 508, 670, 1923
HindII GTYRAC 4 cut(s) 430, 508, 670, 1923
HindIII AAGCTT 1 cut(s) 1014
HphI GGTGA 3 cut(s) 273, 1103, 1219
Hpy166II GTNNAC 7 cut(s) 430, 508, 640, 670, 895, 1048, 1923
Hpy8I GTNNAC 7 cut(s) 430, 508, 640, 670, 895, 1048, 1923
HpyCH4III ACNGT 7 cut(s) 139, 225, 427, 1115, 1314, 1456, 1954
HpyCH4IV ACGT 3 cut(s) 504, 959, 1772
HpyCH4V TGCA 9 cut(s) 22, 66, 715, 740, 985, 1003, 1378, 1651, 2117
HpyF10VI GCNNNNNNNGC 8 cut(s) 72, 741, 756, 1188, 1218, 1225, 1281, 1536
HpyF3I CTNAG 2 cut(s) 813, 1644
HpySE526I ACGT 3 cut(s) 504, 959, 1772
Hsp92I GRCGYC 3 cut(s) 432, 2166, 2264
Hsp92II CATG 3 cut(s) 70, 1522, 1720
HspAI GCGC 6 cut(s) 294, 830, 1219, 1271, 1273, 2264
KasI GGCGCC 1 cut(s) 2263
Kpn2I TCCGGA 1 cut(s) 612
Lsp1109I GCAGC 8 cut(s) 796, 997, 1280, 1342, 1542, 1614, 1737, 2218
LweI GCATC 1 cut(s) 912
MaeI CTAG 3 cut(s) 1167, 1296, 1877
MaeII ACGT 3 cut(s) 504, 959, 1772
MaeIII GTNAC 1 cut(s) 279
MflI RGATCY 7 cut(s) 265, 459, 617, 646, 790, 1824, 2224
MhlI GDGCHC 8 cut(s) 761, 773, 1106, 1214, 1265, 1359, 1607, 1674
MluCI AATT 8 cut(s) 191, 679, 724, 1030, 1840, 1962, 2028, 2075
Mly113I GGCGCC 1 cut(s) 2264
MmeI TCCRAC 8 cut(s) 138, 295, 525, 1590, 1884, 2016, 2187, 2268
MroI TCCGGA 1 cut(s) 612
MroXI GAANNNNTTC 1 cut(s) 842
MseI TTAA 2 cut(s) 606, 1029
MslI CAYNNNNRTG 1 cut(s) 1517
MspA1I CMGCKG 1 cut(s) 1333
MspR9I CCNGG 7 cut(s) 35, 869, 1009, 1265, 1435, 1471, 2054
MvaI CCWGG 4 cut(s) 35, 1009, 1435, 1471
MvnI CGCG 4 cut(s) 294, 623, 1273, 1275
MwoI GCNNNNNNNGC 8 cut(s) 72, 741, 756, 1188, 1218, 1225, 1281, 1536
NarI GGCGCC 1 cut(s) 2264
NciI CCSGG 3 cut(s) 869, 1265, 2054
NdeI CATATG 1 cut(s) 2090
NlaIII CATG 3 cut(s) 70, 1522, 1720
NlaIV GGNNCC 8 cut(s) 222, 312, 772, 873, 1244, 1262, 1780, 2265
NmeAIII GCCGAG 5 cut(s) 110, 833, 1125, 1173, 1807
NmuCI GTSAC 1 cut(s) 279
NspV TTCGAA 1 cut(s) 1062
PaeR7I CTCGAG 1 cut(s) 1928
PagI TCATGA 1 cut(s) 1716
PasI CCCWGGG 1 cut(s) 1470
PauI GCGCGC 1 cut(s) 1271
PceI AGGCCT 1 cut(s) 996
PcsI WCGNNNNNNNCGW 7 cut(s) 143, 371, 1103, 1145, 1806, 1965, 2160
PdmI GAANNNNTTC 1 cut(s) 842
PfeI GAWTC 6 cut(s) 930, 1064, 1125, 1346, 1423, 2049
PflFI GACNNNGTC 1 cut(s) 1952
PfoI TCCNGGA 3 cut(s) 867, 1007, 2052
PinAI ACCGGT 1 cut(s) 660
Ple19I CGATCG 6 cut(s) 94, 239, 542, 1054, 1323, 2154
PluTI GGCGCC 1 cut(s) 2267
PpuMI RGGWCCY 1 cut(s) 2110
PshAI GACNNNNGTC 1 cut(s) 277
PshBI ATTAAT 1 cut(s) 1029
Psp124BI GAGCTC 3 cut(s) 761, 1106, 1607
Psp5II RGGWCCY 1 cut(s) 2110
Psp6I CCWGG 4 cut(s) 33, 1007, 1433, 1469
PspFI CCCAGC 1 cut(s) 545
PspGI CCWGG 4 cut(s) 33, 1007, 1433, 1469
PspN4I GGNNCC 8 cut(s) 222, 312, 772, 873, 1244, 1262, 1780, 2265
PspPI GGNCC 2 cut(s) 1310, 2110
PspPPI RGGWCCY 1 cut(s) 2110
PstI CTGCAG 1 cut(s) 987
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 7 cut(s) 265, 459, 617, 646, 790, 1824, 2224
PsyI GACNNNGTC 1 cut(s) 1952
PteI GCGCGC 1 cut(s) 1271
PvuI CGATCG 6 cut(s) 94, 239, 542, 1054, 1323, 2154
RsaI GTAC 3 cut(s) 659, 1239, 1589
RsaNI GTAC 3 cut(s) 658, 1238, 1588
RseI CAYNNNNRTG 1 cut(s) 1517
SacI GAGCTC 3 cut(s) 761, 1106, 1607
SalI GTCGAC 3 cut(s) 428, 506, 668
SaqAI TTAA 2 cut(s) 606, 1029
Sau96I GGNCC 2 cut(s) 1310, 2110
ScrFI CCNGG 7 cut(s) 35, 869, 1009, 1265, 1435, 1471, 2054
SduI GDGCHC 8 cut(s) 761, 773, 1106, 1214, 1265, 1359, 1607, 1674
SfaNI GCATC 1 cut(s) 912
SfcI CTRYAG 2 cut(s) 983, 2248
SfoI GGCGCC 1 cut(s) 2265
Sfr274I CTCGAG 1 cut(s) 1928
SfuI TTCGAA 1 cut(s) 1062
SgrDI CGTCGACG 3 cut(s) 428, 506, 668
SinI GGWCC 2 cut(s) 1310, 2110
SlaI CTCGAG 1 cut(s) 1928
SmiMI CAYNNNNRTG 1 cut(s) 1517
SmlI CTYRAG 1 cut(s) 1928
SmoI CTYRAG 1 cut(s) 1928
Sse9I AATT 8 cut(s) 191, 679, 724, 1030, 1840, 1962, 2028, 2075
SseBI AGGCCT 1 cut(s) 996
SspDI GGCGCC 1 cut(s) 2263
SspI AATATT 1 cut(s) 1508
SspMI CTAG 3 cut(s) 1167, 1296, 1877
SstI GAGCTC 3 cut(s) 761, 1106, 1607
StuI AGGCCT 1 cut(s) 996
StyD4I CCNGG 7 cut(s) 33, 867, 1007, 1263, 1433, 1469, 2052
TaaI ACNGT 7 cut(s) 139, 225, 427, 1115, 1314, 1456, 1954
TaiI ACGT 3 cut(s) 507, 962, 1775
TaqII GACCGA 2 cut(s) 249, 1986
TasI AATT 8 cut(s) 191, 679, 724, 1030, 1840, 1962, 2028, 2075
TatI WGTACW 1 cut(s) 1587
TauI GCSGC 6 cut(s) 476, 1121, 1144, 1147, 1284, 1421
TfiI GAWTC 6 cut(s) 930, 1064, 1125, 1346, 1423, 2049
Tru1I TTAA 2 cut(s) 606, 1029
Tru9I TTAA 2 cut(s) 606, 1029
TscAI CASTG 1 cut(s) 1380
TseFI GTSAC 1 cut(s) 279
TseI GCWGC 8 cut(s) 784, 985, 1268, 1330, 1530, 1627, 1725, 2231
Tsp45I GTSAC 1 cut(s) 279
TspDTI ATGAA 3 cut(s) 1097, 1478, 1733
TspGWI ACGGA 9 cut(s) 267, 278, 525, 687, 1255, 1580, 1612, 1980, 2148
TspRI CASTG 1 cut(s) 1380
Tth111I GACNNNGTC 1 cut(s) 1952
VpaK11BI GGWCC 2 cut(s) 1310, 2110
VspI ATTAAT 1 cut(s) 1029
XapI RAATTY 2 cut(s) 1962, 2028
XhoI CTCGAG 1 cut(s) 1928
XmiI GTMKAC 5 cut(s) 429, 507, 669, 894, 1047
XmnI GAANNNNTTC 1 cut(s) 842
XspI CTAG 3 cut(s) 1167, 1296, 1877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.