Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009362

Physical Location & Seq

Genomic Coordinates Reverse (-)

20594678 .. 20597644

Gene Structure 2967 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g25330.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGACGGGAAGAAGCTCGAGTACAAAGGTGAGGAGGCATTGAAGGAGATTGAGAGGCTCACTTCAAAGGCTGATAAAGTTCAGCTGAACATTTTGAAAGAGATCTTAACCAGAAATGTGGAGACTGAGTATCTGAGCAAGTACATGAAGGGATCACAAGAAGTTGCAGATTTTAAGAAGTGTGTTCCTGTCATTACTTACAAGGAGATCCAACCTTATATCCAAAGGATAGCTAATGGTGAAGACTCCTCTCTCATAACTGGTCACCCAATAACTGAAATGCTATGCAGCTCAGGAACTTCAGGAAGAGAGCCTAAGATGATGCCAACAATCGCTGAAGATCTCGACCGTCGAACCTTTATCTACAATCTCATTATGCCAATTATCAACCAGTATGTTCCTGATCTGGACAAGGGCAAGGCCATGTTCCTCTACTTTGTCAAGGCAGAGATGTCGACGCCGTGCGGCTTACCGGCACGGACAGTCCTCACCAGCTACTACAAGAGCAACCACTTCAAGTGCCGAGGCGAAGACCCTTTCAACAACTGTACTAGCCCTGACCAGACCGTTTTTTGCAACGACAGCAATCAAAGCATGTATTGCCAGCTCCTCTCTGGCCTACTCCACCGCCATCAAGTCCTGAGGCTCGGGGCCGTTTTCGCCTCAGCTTTTCTACGAGCCATTTCGTTCCTCGAACGAAACTGGGTCAATTTCTGCAATGACATACGAACCGGGAAGTTGGATCCCCTGATCACAGACCCTGATTGCCGCAAATCCATGGCTAAAATTCTCTCCTCACCTGATCCTGACCTGGCTGACGAGATCAGCAGCATTTGTAGCCTTCAATCCTGGAAGGGAATTCTGTGCCAGCTATGGCCTAAGGCTAAGTACATTGAAGCCGTTGTCACTGGATCCATGGCGCAGTACATACCGGCTTTGGAGTATTACAGTGATGGAAAGCTTCCATTAGTCGGCACCATGTATGCATCTTCAGAATGTTACTTTGGTGTGAACTTGAAGCCCTTCTGTGACCCTGCTGATGTGGCGTTCACCCTGATGCCCAATATGGGGTACTTTGAGTTTATACCATTGGGAAGAGGAGAGAATGGGACAAGCAGGGTGATGGACATTGAAGAGGATGAGGATGAGGATGTGCTTAGTGACAAGCTTGTGGACTTGGTGCATGTCAAGCTGGGCGGTTACTATGAATTGGTGGTAACAACCTTTGCTGGGTTATATCGTTATCGTATTGGTGATGTGCTACAAGTCACCGGATTTCACAATCAAGCCCCGCAATTCCGGTTCATATGTAGGAGGAATGTTGTTCTAAGCATCGACACTGACAAAACTAATGAAGAGGACTTGCATAGGAGCATTACCGTGGCCAAAAAGTTGTTAGAGCCTTTCAATGCTCTTCTTGTGGAGTACACGAGCTATGCCGATACGTCGCGCGTTCCAGGCCACTACGTAATGTATTGGGAGATCGTACACCACCATGGATCGCTGTCACCGTCACCAACGGTGGACTCCTTGATCAACAATGTAGTCACGCCACTAGATGATAAGGTGCTCCAAGAATGTTGCATCGCAGTGGAGGAGGAGCTTGATTACATATACCGTCGATGCCGCTCGCACGACAAGTCTGTAGGGCCGCTCGAGATACGTGTTGTGGAGCCTGGCACATTTGAGGCATTGATGGACTACTTCATCAACCAAGGGGGTTCCATCAATCAGTATAAGACGCCGAGGTGTATCAAGTCTAAGAAGGCTCTGAAGCTGCTTGATTCTAATGTTAAGGCTCGTTACTTTAGTCCGAGAGATCCTCATTGGAGTCCTAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

GH3

Protein Analysis

614

Amino Acids

69.65

Weight (kDa)

5.81

Isoelectric Point (pI)

40.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH3	PF03321	2 - 178	5.2e-63	GH3 family N-terminal domain
GH3_M	PF23571	209 - 297	1e-29	GH3 family central domain
GH3_C	PF23572	312 - 447	8.4e-36	GH3 family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009362)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47750
fragaria_vesca	FvH4_2g25330 FvH4_2g25330 FvH4_2g25330
malus_domestica	MD03G1284700.v1.1 MD11G1304200.v1.1
prunus_persica	Prupe.8G257800_v2.0.a1 Prupe.8G257800_v2.0.a1 Prupe.8G257800_v2.0.a1 Prupe.8G257800_v2.0.a1
pyrus_communis	pycom03g22560
rosa_chinensis	RchiOBHm_Chr6g0293491
rosa_laevigata	RLG00000011945
rosa_multiflora	Rmu_sc0006269.1_g000011
rosa_roxburghii	Rroxscaffold_7G00174130
rosa_rugosa	Rorug06G0235800 Rorug06G0235900
rosa_samantha	Rh6AG348600 Rh6BG356100 Rh6CG362700 Rh6DG349700
rosa_wichuraiana	Rw6G030320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	974
AccBSI	CCGCTC	2 cut(s)	1629, 1654
AccI	GTMKAC	1 cut(s)	455
AccII	CGCG	2 cut(s)	1450, 1452
AciI	CCGC	7 cut(s)	465, 628, 769, 1197, 1292, 1627, 1652
AclWI	GGATC	9 cut(s)	160, 202, 737, 750, 797, 906, 919, 1507, 1814
AcoI	YGGCCR	1 cut(s)	1383
AcsI	RAATTY	2 cut(s)	786, 858
AcuI	CTGAAG	4 cut(s)	285, 357, 975, 1793
AcyI	GRCGYC	2 cut(s)	458, 1742
AfaI	GTAC	8 cut(s)	23, 143, 550, 890, 926, 1073, 1427, 1488
AfiI	CCNNNNNNNGG	4 cut(s)	971, 1067, 1068, 1230
AflIII	ACRYGT	1 cut(s)	1663
AjnI	CCWGG	4 cut(s)	810, 848, 1456, 1675
AjuI	GAANNNNNNNTTGG	2 cut(s)	987, 1019
Alw21I	GWGCWC	1 cut(s)	1572
Alw26I	GTCTC	1 cut(s)	116
AlwI	GGATC	9 cut(s)	160, 202, 737, 750, 797, 906, 919, 1507, 1814
AlwNI	CAGNNNCTG	1 cut(s)	761
Ama87I	CYCGRG	3 cut(s)	17, 647, 1655
AoxI	GGCC	7 cut(s)	420, 616, 651, 875, 1383, 1459, 1649
ApeKI	GCWGC	3 cut(s)	288, 828, 1777
ApoI	RAATTY	2 cut(s)	786, 858
Asp700I	GAANNNNTTC	1 cut(s)	1022
AspLEI	GCGC	2 cut(s)	922, 1452
AspS9I	GGNCC	2 cut(s)	651, 1649
AsuC2I	CCSGG	1 cut(s)	733
AvaI	CYCGRG	3 cut(s)	17, 647, 1655
AxyI	CCTNAGG	2 cut(s)	641, 879
BalI	TGGCCA	1 cut(s)	1385
BamHI	GGATCC	2 cut(s)	742, 911
BanI	GGYRCC	1 cut(s)	974
BarI	GAAGNNNNNNTAC	1 cut(s)	37
BauI	CACGAG	1 cut(s)	1429
BbsI	GAAGAC	2 cut(s)	249, 537
Bbv12I	GWGCWC	1 cut(s)	1572
BbvCI	CCTCAGC	1 cut(s)	664
BbvI	GCAGC	3 cut(s)	300, 840, 1764
BccI	CCATC	5 cut(s)	639, 947, 1117, 1690, 1733
BceAI	ACGGC	3 cut(s)	445, 638, 884
BcgI	CGANNNNNNTGC	2 cut(s)	435, 469
BciT130I	CCWGG	4 cut(s)	812, 850, 1458, 1677
BclI	TGATCA	2 cut(s)	750, 1533
BcnI	CCSGG	1 cut(s)	733
BcoDI	GTCTC	1 cut(s)	116
BfaI	CTAG	2 cut(s)	552, 1556
BfmI	CTRYAG	1 cut(s)	1644
BglII	AGATCT	2 cut(s)	102, 340
BisI	GCNGC	7 cut(s)	289, 466, 769, 829, 1627, 1652, 1778
BlsI	GCNGC	7 cut(s)	290, 467, 770, 830, 1628, 1653, 1779
Bme1390I	CCNGG	5 cut(s)	733, 812, 850, 1458, 1677
BmeT110I	CYCGRG	3 cut(s)	17, 647, 1655
BmgT120I	GGNCC	2 cut(s)	651, 1649
BmiI	GGNNCC	6 cut(s)	652, 744, 913, 976, 1674, 1723
BmrFI	CCNGG	5 cut(s)	733, 812, 850, 1458, 1677
BmrI	ACTGGG	1 cut(s)	712
BmsI	GCATC	6 cut(s)	312, 995, 1047, 1341, 1593, 1613
BmuI	ACTGGG	1 cut(s)	712
BpiI	GAAGAC	2 cut(s)	249, 537
BplI	GAGNNNNNCTC	2 cut(s)	1807, 1839
Bpu10I	CCTNAGC	2 cut(s)	292, 664
BpuMI	CCSGG	1 cut(s)	733
BsaAI	YACGTR	2 cut(s)	1468, 1664
BsaHI	GRCGYC	2 cut(s)	458, 1742
BsaJI	CCNNGG	7 cut(s)	523, 777, 915, 1380, 1495, 1714, 1745
BsaWI	WCCGGW	2 cut(s)	1271, 1299
BsaXI	ACNNNNNCTCC	2 cut(s)	113, 143
Bsc4I	CCNNNNNNNGG	4 cut(s)	971, 1067, 1068, 1230
Bse118I	RCCGGY	2 cut(s)	472, 931
Bse1I	ACTGG	4 cut(s)	265, 391, 707, 913
Bse21I	CCTNAGG	2 cut(s)	641, 879
Bse3DI	GCAATG	1 cut(s)	724
BseBI	CCWGG	4 cut(s)	812, 850, 1458, 1677
BseDI	CCNNGG	7 cut(s)	523, 777, 915, 1380, 1495, 1714, 1745
BseGI	GGATG	3 cut(s)	1144, 1150, 1156
BseLI	CCNNNNNNNGG	4 cut(s)	971, 1067, 1068, 1230
BseMI	GCAATG	1 cut(s)	724
BseMII	CTCAG	5 cut(s)	117, 125, 306, 632, 678
BseNI	ACTGG	4 cut(s)	265, 391, 707, 913
BseRI	GAGGAG	7 cut(s)	47, 238, 599, 784, 1113, 1610, 1613
BseXI	GCAGC	3 cut(s)	300, 840, 1764
BseYI	CCCAGC	2 cut(s)	1192, 1229
Bsh1236I	CGCG	2 cut(s)	1450, 1452
Bsh1285I	CGRYCG	1 cut(s)	349
BshFI	GGCC	7 cut(s)	422, 618, 653, 877, 1385, 1461, 1651
BshNI	GGYRCC	1 cut(s)	974
BsiEI	CGRYCG	1 cut(s)	349
BsiHKAI	GWGCWC	1 cut(s)	1572
BsiHKCI	CYCGRG	3 cut(s)	17, 647, 1655
BsiSI	CCGG	5 cut(s)	473, 732, 932, 1272, 1300
BslFI	GGGAC	1 cut(s)	1123
BslI	CCNNNNNNNGG	4 cut(s)	971, 1067, 1068, 1230
BsmAI	GTCTC	1 cut(s)	116
BsmFI	GGGAC	1 cut(s)	1123
BsnI	GGCC	7 cut(s)	422, 618, 653, 877, 1385, 1461, 1651
BsoBI	CYCGRG	3 cut(s)	17, 647, 1655
Bsp1286I	GDGCHC	1 cut(s)	1572
Bsp19I	CCATGG	3 cut(s)	777, 915, 1495
BspACI	CCGC	7 cut(s)	465, 628, 769, 1197, 1292, 1627, 1652
BspANI	GGCC	7 cut(s)	422, 618, 653, 877, 1385, 1461, 1651
BspCNI	CTCAG	5 cut(s)	118, 126, 305, 633, 677
BspFNI	CGCG	2 cut(s)	1450, 1452
BspLI	GGNNCC	6 cut(s)	652, 744, 913, 976, 1674, 1723
BspPI	GGATC	9 cut(s)	160, 202, 737, 750, 797, 906, 919, 1507, 1814
BspQI	GCTCTTC	1 cut(s)	1419
BspT107I	GGYRCC	1 cut(s)	974
BsrBI	CCGCTC	2 cut(s)	1629, 1654
BsrDI	GCAATG	1 cut(s)	724
BsrFI	RCCGGY	2 cut(s)	472, 931
BsrI	ACTGG	4 cut(s)	265, 391, 707, 913
BssAI	RCCGGY	2 cut(s)	472, 931
BssECI	CCNNGG	7 cut(s)	523, 777, 915, 1380, 1495, 1714, 1745
BssNI	GRCGYC	2 cut(s)	458, 1742
BssSI	CACGAG	1 cut(s)	1429
BssT1I	CCWWGG	4 cut(s)	777, 915, 1495, 1714
Bst2BI	CACGAG	1 cut(s)	1429
Bst2UI	CCWGG	4 cut(s)	812, 850, 1458, 1677
Bst4CI	ACNGT	9 cut(s)	350, 484, 548, 568, 950, 1381, 1512, 1522, 1619
Bst6I	CTCTTC	5 cut(s)	301, 1090, 1128, 1350, 1419
BstACI	GRCGYC	2 cut(s)	458, 1742
BstAPI	GCANNNNNTGC	1 cut(s)	600
BstBAI	YACGTR	2 cut(s)	1468, 1664
BstC8I	GCNNGC	3 cut(s)	605, 869, 1631
BstDSI	CCRYGG	4 cut(s)	777, 915, 1380, 1495
BstEII	GGTNACC	1 cut(s)	263
BstF5I	GGATG	3 cut(s)	1144, 1150, 1156
BstFNI	CGCG	2 cut(s)	1450, 1452
BstHHI	GCGC	2 cut(s)	922, 1452
BstMAI	GTCTC	1 cut(s)	116
BstMCI	CGRYCG	1 cut(s)	349
BstMWI	GCNNNNNNNGC	8 cut(s)	582, 591, 600, 659, 837, 1043, 1189, 1458
BstNI	CCWGG	4 cut(s)	812, 850, 1458, 1677
BstNSI	RCATGY	2 cut(s)	598, 1187
BstPI	GGTNACC	1 cut(s)	263
BstSCI	CCNGG	5 cut(s)	731, 810, 848, 1456, 1675
BstSFI	CTRYAG	1 cut(s)	1644
BstSNI	TACGTA	1 cut(s)	1468
BstUI	CGCG	2 cut(s)	1450, 1452
BstV1I	GCAGC	3 cut(s)	300, 840, 1764
BstV2I	GAAGAC	2 cut(s)	249, 537
BstX2I	RGATCY	6 cut(s)	102, 207, 340, 742, 911, 1819
BstXI	CCANNNNNNTGG	1 cut(s)	118
BstYI	RGATCY	6 cut(s)	102, 207, 340, 742, 911, 1819
Bsu36I	CCTNAGG	2 cut(s)	641, 879
BsuRI	GGCC	7 cut(s)	422, 618, 653, 877, 1385, 1461, 1651
BtgI	CCRYGG	4 cut(s)	777, 915, 1380, 1495
BtgZI	GCGATG	1 cut(s)	1570
BtsCI	GGATG	3 cut(s)	1144, 1150, 1156
BtsI	GCAGTG	1 cut(s)	1596
BtsIMutI	CAGTG	4 cut(s)	906, 955, 1338, 1596
Cac8I	GCNNGC	3 cut(s)	605, 869, 1631
CaiI	CAGNNNCTG	1 cut(s)	761
CfoI	GCGC	2 cut(s)	922, 1452
Cfr10I	RCCGGY	2 cut(s)	472, 931
Cfr13I	GGNCC	2 cut(s)	651, 1649
CseI	GACGC	2 cut(s)	466, 1750
Csp6I	GTAC	8 cut(s)	22, 142, 549, 889, 925, 1072, 1426, 1487
CviAII	CATG	8 cut(s)	145, 424, 595, 778, 916, 979, 1184, 1496
CviQI	GTAC	8 cut(s)	22, 142, 549, 889, 925, 1072, 1426, 1487
EaeI	YGGCCR	1 cut(s)	1383
Eam1104I	CTCTTC	5 cut(s)	301, 1090, 1128, 1350, 1419
EarI	CTCTTC	5 cut(s)	301, 1090, 1128, 1350, 1419
Eco105I	TACGTA	1 cut(s)	1468
Eco130I	CCWWGG	4 cut(s)	777, 915, 1495, 1714
Eco57I	CTGAAG	4 cut(s)	285, 357, 975, 1793
Eco81I	CCTNAGG	2 cut(s)	641, 879
Eco88I	CYCGRG	3 cut(s)	17, 647, 1655
Eco91I	GGTNACC	1 cut(s)	263
EcoO65I	GGTNACC	1 cut(s)	263
EcoRI	GAATTC	1 cut(s)	858
EcoRII	CCWGG	4 cut(s)	810, 848, 1456, 1675
EcoT14I	CCWWGG	4 cut(s)	777, 915, 1495, 1714
EcoT22I	ATGCAT	1 cut(s)	988
ErhI	CCWWGG	4 cut(s)	777, 915, 1495, 1714
FaeI	CATG	8 cut(s)	148, 427, 598, 781, 919, 982, 1187, 1499
FaqI	GGGAC	1 cut(s)	1123
FatI	CATG	8 cut(s)	144, 423, 594, 777, 915, 978, 1183, 1495
FauI	CCCGC	1 cut(s)	1299
FauNDI	CATATG	1 cut(s)	1307
FbaI	TGATCA	2 cut(s)	750, 1533
FblI	GTMKAC	1 cut(s)	455
Fnu4HI	GCNGC	7 cut(s)	289, 466, 769, 829, 1627, 1652, 1778
FokI	GGATG	3 cut(s)	1151, 1157, 1163
Fsp4HI	GCNGC	7 cut(s)	289, 466, 769, 829, 1627, 1652, 1778
FspBI	CTAG	2 cut(s)	552, 1556
GlaI	GCGC	2 cut(s)	921, 1451
GluI	GCNGC	7 cut(s)	289, 466, 769, 829, 1627, 1652, 1778
GsaI	CCCAGC	2 cut(s)	1196, 1233
HaeIII	GGCC	7 cut(s)	422, 618, 653, 877, 1385, 1461, 1651
HapII	CCGG	5 cut(s)	473, 732, 932, 1272, 1300
HgaI	GACGC	2 cut(s)	466, 1750
HhaI	GCGC	2 cut(s)	922, 1452
Hin1I	GRCGYC	2 cut(s)	458, 1742
Hin1II	CATG	8 cut(s)	148, 427, 598, 781, 919, 982, 1187, 1499
Hin6I	GCGC	2 cut(s)	920, 1450
HinP1I	GCGC	2 cut(s)	920, 1450
HincII	GTYRAC	1 cut(s)	456
HindII	GTYRAC	1 cut(s)	456
HindIII	AAGCTT	2 cut(s)	959, 1166
HinfI	GANTC	4 cut(s)	245, 1526, 1784, 1831
HpaII	CCGG	5 cut(s)	473, 732, 932, 1272, 1300
Hpy166II	GTNNAC	7 cut(s)	456, 1012, 1050, 1174, 1428, 1489, 1525
Hpy188I	TCNGA	4 cut(s)	135, 994, 1773, 1815
Hpy188III	TCNNGA	8 cut(s)	294, 303, 344, 401, 407, 640, 806, 1657
Hpy8I	GTNNAC	7 cut(s)	456, 1012, 1050, 1174, 1428, 1489, 1525
Hpy99I	CGWCG	4 cut(s)	354, 460, 1450, 1623
HpyAV	CCTTC	6 cut(s)	37, 142, 847, 851, 1033, 1759
HpyCH4III	ACNGT	9 cut(s)	350, 484, 548, 568, 950, 1381, 1512, 1522, 1619
HpyCH4IV	ACGT	3 cut(s)	1445, 1467, 1663
HpyCH4V	TGCA	8 cut(s)	167, 288, 576, 717, 986, 1183, 1366, 1584
HpyF10VI	GCNNNNNNNGC	8 cut(s)	582, 591, 600, 659, 837, 1043, 1189, 1458
HpySE526I	ACGT	3 cut(s)	1445, 1467, 1663
Hsp92I	GRCGYC	2 cut(s)	458, 1742
Hsp92II	CATG	8 cut(s)	148, 427, 598, 781, 919, 982, 1187, 1499
HspAI	GCGC	2 cut(s)	920, 1450
Ksp22I	TGATCA	2 cut(s)	750, 1533
LguI	GCTCTTC	1 cut(s)	1419
LmnI	GCTCC	5 cut(s)	612, 1371, 1575, 1600, 1672
Lsp1109I	GCAGC	3 cut(s)	300, 840, 1764
LweI	GCATC	6 cut(s)	312, 995, 1047, 1341, 1593, 1613
MaeI	CTAG	2 cut(s)	552, 1556
MaeII	ACGT	3 cut(s)	1445, 1467, 1663
MbiI	CCGCTC	2 cut(s)	1629, 1654
MflI	RGATCY	6 cut(s)	102, 207, 340, 742, 911, 1819
MhlI	GDGCHC	1 cut(s)	1572
MlsI	TGGCCA	1 cut(s)	1385
MluCI	AATT	6 cut(s)	381, 709, 786, 858, 1208, 1295
MluNI	TGGCCA	1 cut(s)	1385
MlyI	GAGTC	3 cut(s)	239, 1520, 1840
MmeI	TCCRAC	2 cut(s)	235, 720
Mox20I	TGGCCA	1 cut(s)	1385
Mph1103I	ATGCAT	1 cut(s)	988
MroXI	GAANNNNTTC	1 cut(s)	1022
MscI	TGGCCA	1 cut(s)	1385
MseI	TTAA	3 cut(s)	107, 174, 1794
MslI	CAYNNNNRTG	4 cut(s)	1055, 1379, 1494, 1589
Msp20I	TGGCCA	1 cut(s)	1385
MspA1I	CMGCKG	1 cut(s)	85
MspI	CCGG	5 cut(s)	473, 732, 932, 1272, 1300
MspR9I	CCNGG	5 cut(s)	733, 812, 850, 1458, 1677
MvaI	CCWGG	4 cut(s)	812, 850, 1458, 1677
MvnI	CGCG	2 cut(s)	1450, 1452
MwoI	GCNNNNNNNGC	8 cut(s)	582, 591, 600, 659, 837, 1043, 1189, 1458
NciI	CCSGG	1 cut(s)	733
NcoI	CCATGG	3 cut(s)	777, 915, 1495
NdeI	CATATG	1 cut(s)	1307
NlaIII	CATG	8 cut(s)	148, 427, 598, 781, 919, 982, 1187, 1499
NlaIV	GGNNCC	6 cut(s)	652, 744, 913, 976, 1674, 1723
NmeAIII	GCCGAG	2 cut(s)	548, 1770
NmuCI	GTSAC	8 cut(s)	263, 904, 1028, 1160, 1267, 1506, 1512, 1546
NsiI	ATGCAT	1 cut(s)	988
NspI	RCATGY	2 cut(s)	598, 1187
PaeR7I	CTCGAG	2 cut(s)	17, 1655
PciSI	GCTCTTC	1 cut(s)	1419
PcsI	WCGNNNNNNNCGW	1 cut(s)	1508
PdmI	GAANNNNTTC	1 cut(s)	1022
PfeI	GAWTC	1 cut(s)	1784
PfoI	TCCNGGA	1 cut(s)	848
PkrI	GCNGC	7 cut(s)	290, 467, 770, 830, 1628, 1653, 1779
PleI	GAGTC	3 cut(s)	239, 1520, 1839
PpsI	GAGTC	3 cut(s)	239, 1520, 1839
Ppu21I	YACGTR	2 cut(s)	1468, 1664
Psp6I	CCWGG	4 cut(s)	810, 848, 1456, 1675
PspEI	GGTNACC	1 cut(s)	263
PspFI	CCCAGC	2 cut(s)	1192, 1229
PspGI	CCWGG	4 cut(s)	810, 848, 1456, 1675
PspN4I	GGNNCC	6 cut(s)	652, 744, 913, 976, 1674, 1723
PspPI	GGNCC	2 cut(s)	651, 1649
PspXI	VCTCGAGB	1 cut(s)	17
PstNI	CAGNNNCTG	1 cut(s)	761
PsuI	RGATCY	6 cut(s)	102, 207, 340, 742, 911, 1819
PvuII	CAGCTG	1 cut(s)	85
RsaI	GTAC	8 cut(s)	23, 143, 550, 890, 926, 1073, 1427, 1488
RsaNI	GTAC	8 cut(s)	22, 142, 549, 889, 925, 1072, 1426, 1487
RseI	CAYNNNNRTG	4 cut(s)	1055, 1379, 1494, 1589
SalI	GTCGAC	1 cut(s)	454
SapI	GCTCTTC	1 cut(s)	1419
SaqAI	TTAA	3 cut(s)	107, 174, 1794
SatI	GCNGC	7 cut(s)	289, 466, 769, 829, 1627, 1652, 1778
Sau96I	GGNCC	2 cut(s)	651, 1649
SchI	GAGTC	3 cut(s)	239, 1520, 1840
ScrFI	CCNGG	5 cut(s)	733, 812, 850, 1458, 1677
SduI	GDGCHC	1 cut(s)	1572
SfaNI	GCATC	6 cut(s)	312, 995, 1047, 1341, 1593, 1613
SfcI	CTRYAG	1 cut(s)	1644
Sfr274I	CTCGAG	2 cut(s)	17, 1655
SlaI	CTCGAG	2 cut(s)	17, 1655
SmiMI	CAYNNNNRTG	4 cut(s)	1055, 1379, 1494, 1589
SmlI	CTYRAG	2 cut(s)	17, 1655
SmoI	CTYRAG	2 cut(s)	17, 1655
SnaBI	TACGTA	1 cut(s)	1468
Sse9I	AATT	6 cut(s)	381, 709, 786, 858, 1208, 1295
SsiI	CCGC	7 cut(s)	465, 628, 769, 1197, 1292, 1627, 1652
SspMI	CTAG	2 cut(s)	552, 1556
StyD4I	CCNGG	5 cut(s)	731, 810, 848, 1456, 1675
StyI	CCWWGG	4 cut(s)	777, 915, 1495, 1714
TaaI	ACNGT	9 cut(s)	350, 484, 548, 568, 950, 1381, 1512, 1522, 1619
TaiI	ACGT	3 cut(s)	1448, 1470, 1666
TaqI	TCGA	8 cut(s)	18, 345, 352, 455, 693, 1335, 1621, 1656
TasI	AATT	6 cut(s)	381, 709, 786, 858, 1208, 1295
TatI	WGTACW	6 cut(s)	21, 141, 548, 888, 924, 1425
TauI	GCSGC	4 cut(s)	468, 771, 1629, 1654
TfiI	GAWTC	1 cut(s)	1784
Tru1I	TTAA	3 cut(s)	107, 174, 1794
Tru9I	TTAA	3 cut(s)	107, 174, 1794
TscAI	CASTG	4 cut(s)	913, 955, 1345, 1596
TseFI	GTSAC	8 cut(s)	263, 904, 1028, 1160, 1267, 1506, 1512, 1546
TseI	GCWGC	3 cut(s)	288, 828, 1777
Tsp45I	GTSAC	8 cut(s)	263, 904, 1028, 1160, 1267, 1506, 1512, 1546
TspDTI	ATGAA	5 cut(s)	161, 1221, 1294, 1368, 1696
TspGWI	ACGGA	1 cut(s)	493
TspRI	CASTG	4 cut(s)	913, 955, 1345, 1596
XapI	RAATTY	2 cut(s)	786, 858
XceI	RCATGY	2 cut(s)	598, 1187
XcmI	CCANNNNNNNNNTGG	1 cut(s)	611
XhoI	CTCGAG	2 cut(s)	17, 1655
XmiI	GTMKAC	1 cut(s)	455
XmnI	GAANNNNTTC	1 cut(s)	1022
XspI	CTAG	2 cut(s)	552, 1556
Zsp2I	ATGCAT	1 cut(s)	988

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.