Rorug06G0235800

Indole-3-acetic acid-amido synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009362
Physical Location & Seq
Forward (+)
37803382 .. 37804670
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0235800.1

Sequence Viewer

Length: 744 bp
ATGGGTGAGAGACCATCTTACTCTTGGAGGAGTATTATGGAGGCTAAGCCAGTCTTGCAAGCTGGATTATCTTGGAGGATTGGTAATGGTGCTTCTGTTGGCATTTGGGACTTGGATTGGATATCTTCTGTTTCTCATCATGCTTTAATCTGGCCTCCGGATACTGGATCCGTATGGTCTAGTTTGGAGGGCCTTGTGGAAGCCAAAATTCCCAGCAAGGTGGCCATATTTGGGTGGAGAGCAGTACATAATCTCCTCCGTACTCGTGCAGCTCTGTCAACCAAAGGTTACTCAGCATTTGGAGGAATAGGAATGATAAGTTGTGGAGGGATCGATCTCAAACAGGGCCTGGAATTGTGGCGTCTGCTATGGCCTGATTTGAGGAGTGCTCCCGAGTTCAGTCGGATTTTGATGTATGGAGGTACAAGTGGTGTTATAAGGAATGCTTCTGGACATTTCATTGCTGCCTTTTCTTACAGATCAGATTATGTAACCTCTTCGTTGCATACTGAATTGTTGGCTGTAAAGTATGGATTGGAGTTCCTACATGCCTTGAATGTTACAAAGGCTGTGATTGAGAGCGATTGCCTACATGCAGTTCAAGCCATCAACTCTTTGGTTGGGGATCTGTCTGAATTAGTAGCTTTGATTTCAGAAATCAAAGGCCTAGCTAGTGAATGCTTTAGGGGGAGGTTACAGTCTGTTTCGCTCCTCGACAAGCAAACATGGTTGCTCATCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GH3

Protein Analysis

247

Amino Acids

27.0

Weight (kDa)

7.78

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 139 - 228 5.2e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 437
AccIII TCCGGA 1 cut(s) 157
AclWI GGATC 4 cut(s) 162, 175, 338, 633
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 361
AfaI GTAC 3 cut(s) 246, 262, 424
AfiI CCNNNNNNNGG 2 cut(s) 164, 231
AgsI TTSAA 2 cut(s) 556, 602
AjnI CCWGG 1 cut(s) 348
AluBI AGCT 4 cut(s) 62, 272, 644, 671
AluI AGCT 4 cut(s) 62, 272, 644, 671
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 1 cut(s) 4
AlwI GGATC 4 cut(s) 162, 175, 338, 633
AlwNI CAGNNNCTG 1 cut(s) 349
Ama87I CYCGRG 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 6 cut(s) 152, 190, 222, 346, 371, 664
ApeKI GCWGC 2 cut(s) 269, 464
ApoI RAATTY 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 190, 346
AsuHPI GGTGA 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 392
BalI TGGCCA 1 cut(s) 224
BamHI GGATCC 1 cut(s) 167
BarI GAAGNNNNNNTAC 2 cut(s) 76, 108
BauI CACGAG 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 2 cut(s) 281, 451
BccI CCATC 2 cut(s) 22, 614
BciT130I CCWGG 1 cut(s) 350
BciVI GTATCC 1 cut(s) 154
BcoDI GTCTC 1 cut(s) 4
BfaI CTAG 4 cut(s) 180, 668, 672, 742
BfuI GTATCC 1 cut(s) 154
BisI GCNGC 2 cut(s) 270, 465
BlpI GCTNAGC 1 cut(s) 45
BlsI GCNGC 2 cut(s) 271, 466
Bme1390I CCNGG 1 cut(s) 350
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 2 cut(s) 190, 346
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 1 cut(s) 350
BplI GAGNNNNNCTC 2 cut(s) 373, 405
Bpu1102I GCTNAGC 1 cut(s) 45
Bsa29I ATCGAT 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 361
BsaI GGTCTC 1 cut(s) 4
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 237, 267
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 231
Bse1I ACTGG 2 cut(s) 50, 169
Bse3DI GCAATG 1 cut(s) 459
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 1 cut(s) 350
BseCI ATCGAT 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 164, 231
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 2 cut(s) 50, 169
BseRI GAGGAG 4 cut(s) 43, 245, 397, 701
BseXI GCAGC 2 cut(s) 281, 451
BseYI CCCAGC 1 cut(s) 212
BsgI GTGCAG 1 cut(s) 288
BshFI GGCC 6 cut(s) 154, 192, 224, 348, 373, 666
BshVI ATCGAT 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 391
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 164, 231
BsmAI GTCTC 1 cut(s) 4
BsmFI GGGAC 1 cut(s) 122
BsmI GAATGC 2 cut(s) 448, 683
BsnI GGCC 6 cut(s) 154, 192, 224, 348, 373, 666
Bso31I GGTCTC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 1 cut(s) 391
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 5 cut(s) 167, 330, 334, 479, 625
Bsp1720I GCTNAGC 1 cut(s) 45
BspANI GGCC 6 cut(s) 154, 192, 224, 348, 373, 666
BspCNI CTCAG 1 cut(s) 305
BspDI ATCGAT 1 cut(s) 333
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 169
BspPI GGATC 4 cut(s) 162, 175, 338, 633
BspTNI GGTCTC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 2 cut(s) 50, 169
BssMI GATC 5 cut(s) 167, 330, 334, 479, 625
BssNI GRCGYC 1 cut(s) 361
BssSI CACGAG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 350
Bst4CI ACNGT 1 cut(s) 699
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 1 cut(s) 361
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 45, 292
BstKTI GATC 5 cut(s) 170, 333, 337, 482, 628
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 5 cut(s) 167, 330, 334, 479, 625
BstMWI GCNNNNNNNGC 2 cut(s) 55, 602
BstNI CCWGG 1 cut(s) 350
BstNSI RCATGY 2 cut(s) 551, 596
BstSCI CCNGG 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 281, 451
BstX2I RGATCY 2 cut(s) 167, 625
BstXI CCANNNNNNTGG 1 cut(s) 220
BstYI RGATCY 2 cut(s) 167, 625
Bsu15I ATCGAT 1 cut(s) 333
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 6 cut(s) 154, 192, 224, 348, 373, 666
BsuTUI ATCGAT 1 cut(s) 333
BtgZI GCGATG 1 cut(s) 721
Cac8I GCNNGC 1 cut(s) 60
CaiI CAGNNNCTG 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 190, 346
ClaI ATCGAT 1 cut(s) 333
CseI GACGC 1 cut(s) 350
Csp6I GTAC 3 cut(s) 245, 261, 423
CviAII CATG 4 cut(s) 140, 548, 593, 726
CviQI GTAC 3 cut(s) 245, 261, 423
DdeI CTNAG 2 cut(s) 45, 292
DpnI GATC 5 cut(s) 169, 332, 336, 481, 627
DpnII GATC 5 cut(s) 167, 330, 334, 479, 625
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 502
Eco147I AGGCCT 1 cut(s) 666
Eco31I GGTCTC 1 cut(s) 4
Eco32I GATATC 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 392
EcoO109I RGGNCCY 2 cut(s) 190, 346
EcoRII CCWGG 1 cut(s) 348
EcoRV GATATC 1 cut(s) 123
FaeI CATG 4 cut(s) 143, 551, 596, 729
FalI AAGNNNNNCTT 4 cut(s) 38, 70, 430, 462
FaqI GGGAC 1 cut(s) 122
FatI CATG 4 cut(s) 139, 547, 592, 725
Fnu4HI GCNGC 2 cut(s) 270, 465
Fsp4HI GCNGC 2 cut(s) 270, 465
FspBI CTAG 4 cut(s) 180, 668, 672, 742
GluI GCNGC 2 cut(s) 270, 465
GsaI CCCAGC 1 cut(s) 216
HaeIII GGCC 6 cut(s) 154, 192, 224, 348, 373, 666
HapII CCGG 1 cut(s) 158
HgaI GACGC 1 cut(s) 350
Hin1I GRCGYC 1 cut(s) 361
Hin1II CATG 4 cut(s) 143, 551, 596, 729
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HpaII CCGG 1 cut(s) 158
HphI GGTGA 1 cut(s) 17
Hpy166II GTNNAC 1 cut(s) 279
Hpy188I TCNGA 4 cut(s) 405, 484, 634, 655
Hpy188III TCNNGA 3 cut(s) 158, 392, 450
Hpy8I GTNNAC 1 cut(s) 279
HpyCH4III ACNGT 1 cut(s) 699
HpyCH4V TGCA 4 cut(s) 58, 269, 505, 596
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 602
HpyF3I CTNAG 2 cut(s) 45, 292
Hsp92I GRCGYC 1 cut(s) 361
Hsp92II CATG 4 cut(s) 143, 551, 596, 729
Kpn2I TCCGGA 1 cut(s) 157
Kzo9I GATC 5 cut(s) 167, 330, 334, 479, 625
LmnI GCTCC 2 cut(s) 394, 714
Lsp1109I GCAGC 2 cut(s) 281, 451
MaeI CTAG 4 cut(s) 180, 668, 672, 742
MaeIII GTNAC 4 cut(s) 287, 490, 559, 693
MalI GATC 5 cut(s) 169, 332, 336, 481, 627
MboI GATC 5 cut(s) 167, 330, 334, 479, 625
MboII GAAGA 2 cut(s) 117, 489
MflI RGATCY 2 cut(s) 167, 625
MhlI GDGCHC 1 cut(s) 391
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 4 cut(s) 207, 353, 512, 635
MluNI TGGCCA 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 383
Mox20I TGGCCA 1 cut(s) 224
MroI TCCGGA 1 cut(s) 157
MscI TGGCCA 1 cut(s) 224
MseI TTAA 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 224
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 1 cut(s) 350
Mva1269I GAATGC 2 cut(s) 448, 683
MvaI CCWGG 1 cut(s) 350
MwoI GCNNNNNNNGC 2 cut(s) 55, 602
NdeII GATC 5 cut(s) 167, 330, 334, 479, 625
NlaIII CATG 4 cut(s) 143, 551, 596, 729
NlaIV GGNNCC 1 cut(s) 169
NspI RCATGY 2 cut(s) 551, 596
PceI AGGCCT 1 cut(s) 666
PctI GAATGC 2 cut(s) 448, 683
PkrI GCNGC 2 cut(s) 271, 466
PsiI TTATAA 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 348
PspFI CCCAGC 1 cut(s) 212
PspGI CCWGG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 2 cut(s) 190, 346
PstNI CAGNNNCTG 1 cut(s) 349
PsuI RGATCY 2 cut(s) 167, 625
RsaI GTAC 3 cut(s) 246, 262, 424
RsaNI GTAC 3 cut(s) 245, 261, 423
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 2 cut(s) 270, 465
Sau3AI GATC 5 cut(s) 167, 330, 334, 479, 625
Sau96I GGNCC 2 cut(s) 190, 346
ScrFI CCNGG 1 cut(s) 350
SduI GDGCHC 1 cut(s) 391
SetI ASST 9 cut(s) 64, 222, 274, 289, 424, 497, 646, 673, 695
Sse9I AATT 4 cut(s) 207, 353, 512, 635
SseBI AGGCCT 1 cut(s) 666
SspMI CTAG 4 cut(s) 180, 668, 672, 742
StuI AGGCCT 1 cut(s) 666
StyD4I CCNGG 1 cut(s) 348
TaaI ACNGT 1 cut(s) 699
TaqI TCGA 2 cut(s) 333, 714
TasI AATT 4 cut(s) 207, 353, 512, 635
TatI WGTACW 1 cut(s) 244
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TseI GCWGC 2 cut(s) 269, 464
TspDTI ATGAA 1 cut(s) 448
TspGWI ACGGA 2 cut(s) 160, 248
XapI RAATTY 1 cut(s) 207
XceI RCATGY 2 cut(s) 551, 596
XcmI CCANNNNNNNNNTGG 2 cut(s) 21, 613
XspI CTAG 4 cut(s) 180, 668, 672, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.