Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0010157

Physical Location & Seq

Genomic Coordinates Forward (+)

21625648 .. 21630457

Gene Structure 4810 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g27160.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGAGTTCCAATAAAATATTAATATTTACAGTCCTCCTCCTCACATCTCAGACAGTCCCGGCGTCGCCGTCGCCGTCTGCTCCGGGAGATCACAGTCACCCGATTTCTGAGCTCGAGCTCGACTGTTGCCTAAACTGCAAGCCAAGCCCCGAATTAACCTCCGCCACCAAGAACTACAGCCGCATCCACCGTGCCCTAGCGACTGCATCGACGGCGACGTCTTGCGAGGTCTGGACGACGGCGTGCTCGGAATCGGTGCTGAGCTTTGCGAAGCAGCCGGAGAACGTGGAGTGGCTGAAGAGGATTAGGCGGAGGATCCATGAGCATCCGGAGCTGGCTTTTGAGGAGGTCGAGACGAGTCGCTTGGTTAGGGCCGAGCTTGACAGGTTGGACATCGATTACCGGTTTCCGTTAGCAACCACCGGGATCCGGGCATGGATCGGAACCGGCGGCCCGCCGTTTGTGGCCGTCAGAGCTGACATGGATGCCCTGCCTATTCAGGAAGCCGTAGAATGGGAACACAAGAGCAAGGTCGCCGGCAAAATGCATGCTTGTGGGCACGACGCACATGTGACCATGCTATTAGGTGCTGCCAAGATCTTGAAGAGCCGTGAACACCTTTTGAAGGGGACTGTAGTATTGATATTTCAACCGGCGGAGGAAGCCGGTAACGGCGCAAAGAGAATGATTGGAGATGGGGCTTTGGAGGATGTGGAGGCCATATTCGCAGCTCATGTATCCCACGAGCATCCCACAGGCATTATTGGGTCGAGGTCTGGTCCTTTGCTAGCTGGCTGTGGCTTCTTTAGAGCTGTTATCACCGGAAGGACTGGCCATGGCGAAAGCCCTCACCACTCTGTCGACGCCGTTTTGGCAGCTGCCGCGGCCGTGATCAGCTTACAGGGAATTGTATCACGCGAAGCGAACCCTCTAGACTCTCAGGTGGTGTCCGTTACAGCTTTTAATGGAGGTGACAAGCTTGACATGATACCTAATAGTGTTGTAATTGGTGGGACTCTCAGGGCTTTCTCTAACACAAGCTTCTATCATCTTCTTCAAAGAATTGAAGAGGTAATTGTGGAACAAGCAAGTGTTTACAGATGCTCGGGAACGGTGGATTTCTTCAAAGACAAGCACACCATATATCCACCGACGGTGAACGACGAGAAGATGCACGAACATGTGAGGAAAGTAGCCATGGATTTGTTGGGTCCAACAAACTTCAGGGTGGTGCCGCCTATGATGGGTGCGGAGGACTTCTCCTTCTACTCAGAGGTGATCCCTGCCGGCTTCTTCTACATTGGCATAAGGAACGAGACCTTGGGTTCCTTTCACACAGGCCATTCACCTTATTTTTTCATCGACGAAGATGTTTTGCCTATAGGGGCAGCAACTCATGCCACTATTGCCGAAAGATACCTCATCGAGCATGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0006082 GO:0008150 GO:0008152 GO:0009694 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0010112 GO:0010817 GO:0016787 GO:0016810 GO:0019752 GO:0031347 GO:0032101 GO:0032787 GO:0042221 GO:0042445 GO:0043436 GO:0043900 GO:0044237 GO:0044281 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0080134 GO:1901700 GO:1990206

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

479

Amino Acids

52.07

Weight (kDa)

5.93

Isoelectric Point (pI)

40.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	156 - 298	1.3e-28	Peptidase family M20/M25/M40
M20_dimer	PF07687	269 - 361	8.2e-06	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44350
fragaria_vesca	FvH4_2g27160 FvH4_2g27160
malus_domestica	MD08G1161700.v1.1 MD15G1346800.v1.1
prunus_persica	Prupe.1G494400_v2.0.a1 Prupe.1G494400_v2.0.a1
pyrus_communis	pycom08g13900 pycom15g31060
rosa_chinensis	RchiOBHm_Chr6g0296071
rosa_laevigata	RLG00000011736
rosa_multiflora	Rmu_sc0003139.1_g000031 Rmu_sc0004275.1_g000018
rosa_roxburghii	Rroxscaffold_7G00171850
rosa_rugosa	Rorug06G0256900 Rorug06G0257000
rosa_samantha	Rh6AG370300 Rh6BG377400 Rh6CG383300 Rh6DG370100
rosa_wichuraiana	Rw6G032270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	217
AatII	GACGTC	1 cut(s)	221
AccB1I	GGYRCC	1 cut(s)	1231
AccI	GTMKAC	1 cut(s)	861
AccII	CGCG	2 cut(s)	884, 918
AccIII	TCCGGA	1 cut(s)	328
AclWI	GGATC	6 cut(s)	310, 323, 421, 434, 446, 1273
AcoI	YGGCCR	3 cut(s)	465, 832, 885
AcuI	CTGAAG	2 cut(s)	317, 1207
AcyI	GRCGYC	3 cut(s)	62, 218, 864
AfiI	CCNNNNNNNGG	4 cut(s)	429, 513, 625, 1244
AflIII	ACRYGT	2 cut(s)	568, 1180
AgeI	ACCGGT	1 cut(s)	402
AgsI	TTSAA	6 cut(s)	604, 625, 650, 1058, 1067, 1126
AjuI	GAANNNNNNNTTGG	2 cut(s)	1304, 1336
Alw21I	GWGCWC	3 cut(s)	114, 120, 248
Alw26I	GTCTC	2 cut(s)	347, 1310
AlwI	GGATC	6 cut(s)	310, 323, 421, 434, 446, 1273
Ama87I	CYCGRG	2 cut(s)	113, 1105
Aor13HI	TCCGGA	1 cut(s)	328
AoxI	GGCC	7 cut(s)	372, 451, 465, 717, 832, 885, 1339
ApeKI	GCWGC	6 cut(s)	274, 590, 728, 875, 878, 1388
AseI	ATTAAT	1 cut(s)	20
AsiGI	ACCGGT	1 cut(s)	402
AspLEI	GCGC	1 cut(s)	677
AspS9I	GGNCC	4 cut(s)	372, 452, 779, 1211
AsuC2I	CCSGG	4 cut(s)	59, 84, 424, 431
AsuHPI	GGTGA	7 cut(s)	89, 811, 842, 983, 1168, 1288, 1338
AsuNHI	GCTAGC	1 cut(s)	787
AvaI	CYCGRG	2 cut(s)	113, 1105
AvaII	GGWCC	2 cut(s)	779, 1211
BaeGI	GKGCMC	2 cut(s)	196, 561
BalI	TGGCCA	1 cut(s)	834
BamHI	GGATCC	2 cut(s)	315, 426
BanI	GGYRCC	1 cut(s)	1231
BanII	GRGCYC	2 cut(s)	114, 120
BauI	CACGAG	1 cut(s)	743
Bbv12I	GWGCWC	3 cut(s)	114, 120, 248
BbvI	GCAGC	6 cut(s)	286, 577, 740, 865, 887, 1400
BccI	CCATC	2 cut(s)	689, 1237
BciVI	GTATCC	1 cut(s)	748
BclI	TGATCA	1 cut(s)	891
BcnI	CCSGG	4 cut(s)	59, 84, 424, 431
BcoDI	GTCTC	2 cut(s)	347, 1310
BfaI	CTAG	3 cut(s)	197, 788, 932
BfmI	CTRYAG	3 cut(s)	175, 633, 1380
BfuI	GTATCC	1 cut(s)	748
BglI	GCCNNNNNGGC	1 cut(s)	872
BglII	AGATCT	1 cut(s)	597
BlpI	GCTNAGC	1 cut(s)	260
Bme1390I	CCNGG	4 cut(s)	59, 84, 424, 431
Bme18I	GGWCC	2 cut(s)	779, 1211
BmeT110I	CYCGRG	2 cut(s)	113, 1105
BmgT120I	GGNCC	4 cut(s)	372, 452, 779, 1211
BmiI	GGNNCC	6 cut(s)	317, 428, 445, 1212, 1233, 1327
BmrFI	CCNGG	4 cut(s)	59, 84, 424, 431
BmsI	GCATC	7 cut(s)	192, 215, 334, 475, 757, 1091, 1161
BmtI	GCTAGC	1 cut(s)	791
BplI	GAGNNNNNCTC	2 cut(s)	1244, 1276
Bpu1102I	GCTNAGC	1 cut(s)	260
BpuMI	CCSGG	4 cut(s)	59, 84, 424, 431
Bsa29I	ATCGAT	1 cut(s)	396
BsaHI	GRCGYC	3 cut(s)	62, 218, 864
BsaI	GGTCTC	1 cut(s)	1310
BsaJI	CCNNGG	4 cut(s)	835, 882, 1197, 1320
BsaWI	WCCGGW	3 cut(s)	328, 402, 821
Bsc4I	CCNNNNNNNGG	4 cut(s)	429, 513, 625, 1244
Bse118I	RCCGGY	6 cut(s)	402, 446, 536, 652, 665, 1286
Bse1I	ACTGG	1 cut(s)	835
BseAI	TCCGGA	1 cut(s)	328
BseCI	ATCGAT	1 cut(s)	396
BseDI	CCNNGG	4 cut(s)	835, 882, 1197, 1320
BseGI	GGATG	5 cut(s)	183, 325, 490, 715, 748
BseLI	CCNNNNNNNGG	4 cut(s)	429, 513, 625, 1244
BseMII	CTCAG	6 cut(s)	62, 99, 251, 953, 1033, 1284
BseNI	ACTGG	1 cut(s)	835
BseRI	GAGGAG	3 cut(s)	26, 29, 359
BseSI	GKGCMC	2 cut(s)	196, 561
BseX3I	CGGCCG	1 cut(s)	885
BseXI	GCAGC	6 cut(s)	286, 577, 740, 865, 887, 1400
Bsh1236I	CGCG	2 cut(s)	884, 918
Bsh1285I	CGRYCG	1 cut(s)	888
BshFI	GGCC	7 cut(s)	374, 453, 467, 719, 834, 887, 1341
BshNI	GGYRCC	1 cut(s)	1231
BshTI	ACCGGT	1 cut(s)	402
BshVI	ATCGAT	1 cut(s)	396
BsiEI	CGRYCG	1 cut(s)	888
BsiHKAI	GWGCWC	3 cut(s)	114, 120, 248
BsiHKCI	CYCGRG	2 cut(s)	113, 1105
BslFI	GGGAC	3 cut(s)	41, 643, 1027
BslI	CCNNNNNNNGG	4 cut(s)	429, 513, 625, 1244
BsmAI	GTCTC	2 cut(s)	347, 1310
BsmBI	CGTCTC	1 cut(s)	347
BsmFI	GGGAC	3 cut(s)	41, 643, 1027
BsnI	GGCC	7 cut(s)	374, 453, 467, 719, 834, 887, 1341
Bso31I	GGTCTC	1 cut(s)	1310
BsoBI	CYCGRG	2 cut(s)	113, 1105
Bsp1286I	GDGCHC	5 cut(s)	114, 120, 196, 248, 561
Bsp13I	TCCGGA	1 cut(s)	328
Bsp143I	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
Bsp1720I	GCTNAGC	1 cut(s)	260
Bsp19I	CCATGG	2 cut(s)	835, 1197
BspANI	GGCC	7 cut(s)	374, 453, 467, 719, 834, 887, 1341
BspCNI	CTCAG	6 cut(s)	61, 100, 252, 952, 1032, 1283
BspDI	ATCGAT	1 cut(s)	396
BspEI	TCCGGA	1 cut(s)	328
BspFNI	CGCG	2 cut(s)	884, 918
BspLI	GGNNCC	6 cut(s)	317, 428, 445, 1212, 1233, 1327
BspOI	GCTAGC	1 cut(s)	791
BspPI	GGATC	6 cut(s)	310, 323, 421, 434, 446, 1273
BspQI	GCTCTTC	1 cut(s)	599
BspT107I	GGYRCC	1 cut(s)	1231
BspTNI	GGTCTC	1 cut(s)	1310
BsrFI	RCCGGY	6 cut(s)	402, 446, 536, 652, 665, 1286
BsrI	ACTGG	1 cut(s)	835
BssAI	RCCGGY	6 cut(s)	402, 446, 536, 652, 665, 1286
BssECI	CCNNGG	4 cut(s)	835, 882, 1197, 1320
BssMI	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
BssNI	GRCGYC	3 cut(s)	62, 218, 864
BssSI	CACGAG	1 cut(s)	743
BssT1I	CCWWGG	3 cut(s)	835, 1197, 1320
Bst2BI	CACGAG	1 cut(s)	743
Bst4CI	ACNGT	8 cut(s)	31, 55, 95, 125, 191, 634, 1114, 1156
Bst6I	CTCTTC	3 cut(s)	293, 599, 1062
BstACI	GRCGYC	3 cut(s)	62, 218, 864
BstAPI	GCANNNNNTGC	1 cut(s)	1397
BstC8I	GCNNGC	9 cut(s)	140, 244, 336, 455, 538, 549, 789, 793, 1288
BstDEI	CTNAG	6 cut(s)	48, 108, 260, 939, 1019, 1270
BstDSI	CCRYGG	3 cut(s)	835, 882, 1197
BstENI	CCTNNNNNAGG	1 cut(s)	623
BstF5I	GGATG	5 cut(s)	183, 325, 490, 715, 748
BstFNI	CGCG	2 cut(s)	884, 918
BstHHI	GCGC	1 cut(s)	677
BstKTI	GATC	7 cut(s)	91, 318, 429, 441, 600, 894, 1281
BstMAI	GTCTC	2 cut(s)	347, 1310
BstMBI	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
BstMCI	CGRYCG	1 cut(s)	888
BstNSI	RCATGY	3 cut(s)	551, 572, 1184
BstSCI	CCNGG	4 cut(s)	57, 82, 422, 429
BstSFI	CTRYAG	3 cut(s)	175, 633, 1380
BstSLI	GKGCMC	2 cut(s)	196, 561
BstUI	CGCG	2 cut(s)	884, 918
BstV1I	GCAGC	6 cut(s)	286, 577, 740, 865, 887, 1400
BstX2I	RGATCY	3 cut(s)	315, 426, 597
BstYI	RGATCY	3 cut(s)	315, 426, 597
BstZI	CGGCCG	1 cut(s)	885
Bsu15I	ATCGAT	1 cut(s)	396
BsuI	GTATCC	1 cut(s)	748
BsuRI	GGCC	7 cut(s)	374, 453, 467, 719, 834, 887, 1341
BsuTUI	ATCGAT	1 cut(s)	396
BtgI	CCRYGG	3 cut(s)	835, 882, 1197
BtsCI	GGATG	5 cut(s)	183, 325, 490, 715, 748
Cac8I	GCNNGC	9 cut(s)	140, 244, 336, 455, 538, 549, 789, 793, 1288
CfoI	GCGC	1 cut(s)	677
Cfr10I	RCCGGY	6 cut(s)	402, 446, 536, 652, 665, 1286
Cfr13I	GGNCC	4 cut(s)	372, 452, 779, 1211
Cfr42I	CCGCGG	1 cut(s)	885
ClaI	ATCGAT	1 cut(s)	396
CseI	GACGC	3 cut(s)	51, 572, 872
CspAI	ACCGGT	1 cut(s)	402
DdeI	CTNAG	6 cut(s)	48, 108, 260, 939, 1019, 1270
DpnI	GATC	7 cut(s)	90, 317, 428, 440, 599, 893, 1280
DpnII	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
DrdI	GACNNNNNNGTC	1 cut(s)	217
DseDI	GACNNNNNNGTC	1 cut(s)	217
EaeI	YGGCCR	3 cut(s)	465, 832, 885
EagI	CGGCCG	1 cut(s)	885
Eam1104I	CTCTTC	3 cut(s)	293, 599, 1062
EarI	CTCTTC	3 cut(s)	293, 599, 1062
EciI	GGCGGA	3 cut(s)	151, 325, 671
Ecl136II	GAGCTC	2 cut(s)	112, 118
EclXI	CGGCCG	1 cut(s)	885
Eco130I	CCWWGG	3 cut(s)	835, 1197, 1320
Eco24I	GRGCYC	2 cut(s)	114, 120
Eco31I	GGTCTC	1 cut(s)	1310
Eco47I	GGWCC	2 cut(s)	779, 1211
Eco52I	CGGCCG	1 cut(s)	885
Eco53kI	GAGCTC	2 cut(s)	112, 118
Eco57I	CTGAAG	2 cut(s)	317, 1207
Eco88I	CYCGRG	2 cut(s)	113, 1105
EcoICRI	GAGCTC	2 cut(s)	112, 118
EcoNI	CCTNNNNNAGG	1 cut(s)	623
EcoT14I	CCWWGG	3 cut(s)	835, 1197, 1320
EcoT22I	ATGCAT	1 cut(s)	549
EcoT38I	GRGCYC	2 cut(s)	114, 120
ErhI	CCWWGG	3 cut(s)	835, 1197, 1320
Esp3I	CGTCTC	1 cut(s)	347
FaqI	GGGAC	3 cut(s)	41, 643, 1027
FauI	CCCGC	1 cut(s)	462
FbaI	TGATCA	1 cut(s)	891
FblI	GTMKAC	1 cut(s)	861
FokI	GGATG	5 cut(s)	170, 312, 497, 722, 735
FriOI	GRGCYC	2 cut(s)	114, 120
FspBI	CTAG	3 cut(s)	197, 788, 932
GlaI	GCGC	1 cut(s)	676
HaeIII	GGCC	7 cut(s)	374, 453, 467, 719, 834, 887, 1341
HgaI	GACGC	3 cut(s)	51, 572, 872
HhaI	GCGC	1 cut(s)	677
Hin1I	GRCGYC	3 cut(s)	62, 218, 864
Hin6I	GCGC	1 cut(s)	675
HinP1I	GCGC	1 cut(s)	675
HincII	GTYRAC	1 cut(s)	862
HindII	GTYRAC	1 cut(s)	862
HindIII	AAGCTT	2 cut(s)	977, 1039
HinfI	GANTC	4 cut(s)	251, 358, 935, 1015
HphI	GGTGA	7 cut(s)	89, 811, 842, 983, 1168, 1288, 1338
Hpy166II	GTNNAC	4 cut(s)	614, 862, 1096, 1159
Hpy188I	TCNGA	6 cut(s)	51, 109, 250, 443, 473, 1273
Hpy188III	TCNNGA	7 cut(s)	232, 329, 352, 500, 601, 932, 1107
Hpy8I	GTNNAC	4 cut(s)	614, 862, 1096, 1159
HpyAV	CCTTC	3 cut(s)	619, 819, 1273
HpyCH4III	ACNGT	8 cut(s)	31, 55, 95, 125, 191, 634, 1114, 1156
HpyCH4IV	ACGT	2 cut(s)	218, 285
HpyCH4V	TGCA	4 cut(s)	138, 206, 547, 1174
HpyF3I	CTNAG	6 cut(s)	48, 108, 260, 939, 1019, 1270
HpySE526I	ACGT	2 cut(s)	218, 285
Hsp92I	GRCGYC	3 cut(s)	62, 218, 864
HspAI	GCGC	1 cut(s)	675
Kpn2I	TCCGGA	1 cut(s)	328
KroI	GCCGGC	2 cut(s)	536, 1286
KroNI	GCCGGC	2 cut(s)	538, 1288
Ksp22I	TGATCA	1 cut(s)	891
KspI	CCGCGG	1 cut(s)	885
Kzo9I	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
LguI	GCTCTTC	1 cut(s)	599
LmnI	GCTCC	2 cut(s)	85, 331
Lsp1109I	GCAGC	6 cut(s)	286, 577, 740, 865, 887, 1400
LweI	GCATC	7 cut(s)	192, 215, 334, 475, 757, 1091, 1161
MaeI	CTAG	3 cut(s)	197, 788, 932
MaeII	ACGT	2 cut(s)	218, 285
MaeIII	GTNAC	5 cut(s)	95, 571, 668, 952, 971
MalI	GATC	7 cut(s)	90, 317, 428, 440, 599, 893, 1280
MboI	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
MboII	GAAGA	9 cut(s)	310, 616, 1043, 1046, 1079, 1114, 1180, 1285, 1379
MflI	RGATCY	3 cut(s)	315, 426, 597
MhlI	GDGCHC	5 cut(s)	114, 120, 196, 248, 561
MlsI	TGGCCA	1 cut(s)	834
MluCI	AATT	5 cut(s)	152, 906, 1005, 1062, 1074
MluNI	TGGCCA	1 cut(s)	834
MlyI	GAGTC	3 cut(s)	367, 929, 1009
MmeI	TCCRAC	2 cut(s)	369, 1238
Mox20I	TGGCCA	1 cut(s)	834
Mph1103I	ATGCAT	1 cut(s)	549
MroI	TCCGGA	1 cut(s)	328
MroNI	GCCGGC	2 cut(s)	536, 1286
MscI	TGGCCA	1 cut(s)	834
MseI	TTAA	4 cut(s)	20, 155, 963, 1435
MslI	CAYNNNNRTG	2 cut(s)	552, 1179
Msp20I	TGGCCA	1 cut(s)	834
MspA1I	CMGCKG	2 cut(s)	878, 884
MspR9I	CCNGG	4 cut(s)	59, 84, 424, 431
MvnI	CGCG	2 cut(s)	884, 918
NaeI	GCCGGC	2 cut(s)	538, 1288
NciI	CCSGG	4 cut(s)	59, 84, 424, 431
NcoI	CCATGG	2 cut(s)	835, 1197
NdeII	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
NgoMIV	GCCGGC	2 cut(s)	536, 1286
NheI	GCTAGC	1 cut(s)	787
NlaIV	GGNNCC	6 cut(s)	317, 428, 445, 1212, 1233, 1327
NmeAIII	GCCGAG	1 cut(s)	400
NmuCI	GTSAC	3 cut(s)	95, 571, 971
NsiI	ATGCAT	1 cut(s)	549
NspI	RCATGY	3 cut(s)	551, 572, 1184
PaeI	GCATGC	1 cut(s)	551
PaeR7I	CTCGAG	1 cut(s)	113
PciI	ACATGT	2 cut(s)	568, 1180
PciSI	GCTCTTC	1 cut(s)	599
PcsI	WCGNNNNNNNCGW	2 cut(s)	71, 215
PdiI	GCCGGC	2 cut(s)	538, 1288
PfeI	GAWTC	1 cut(s)	251
PfoI	TCCNGGA	1 cut(s)	82
PinAI	ACCGGT	1 cut(s)	402
PleI	GAGTC	3 cut(s)	366, 929, 1009
PpsI	GAGTC	3 cut(s)	366, 929, 1009
PscI	ACATGT	2 cut(s)	568, 1180
PshBI	ATTAAT	1 cut(s)	20
Psp124BI	GAGCTC	2 cut(s)	114, 120
PspN4I	GGNNCC	6 cut(s)	317, 428, 445, 1212, 1233, 1327
PspPI	GGNCC	4 cut(s)	372, 452, 779, 1211
PspXI	VCTCGAGB	1 cut(s)	113
PsuI	RGATCY	3 cut(s)	315, 426, 597
PvuII	CAGCTG	1 cut(s)	878
RseI	CAYNNNNRTG	2 cut(s)	552, 1179
SacI	GAGCTC	2 cut(s)	114, 120
SacII	CCGCGG	1 cut(s)	885
SalI	GTCGAC	1 cut(s)	860
SapI	GCTCTTC	1 cut(s)	599
SaqAI	TTAA	4 cut(s)	20, 155, 963, 1435
Sau3AI	GATC	7 cut(s)	88, 315, 426, 438, 597, 891, 1278
Sau96I	GGNCC	4 cut(s)	372, 452, 779, 1211
SchI	GAGTC	3 cut(s)	367, 929, 1009
ScrFI	CCNGG	4 cut(s)	59, 84, 424, 431
SduI	GDGCHC	5 cut(s)	114, 120, 196, 248, 561
SfaNI	GCATC	7 cut(s)	192, 215, 334, 475, 757, 1091, 1161
SfcI	CTRYAG	3 cut(s)	175, 633, 1380
Sfr274I	CTCGAG	1 cut(s)	113
Sfr303I	CCGCGG	1 cut(s)	885
SgrBI	CCGCGG	1 cut(s)	885
SinI	GGWCC	2 cut(s)	779, 1211
SlaI	CTCGAG	1 cut(s)	113
SmiMI	CAYNNNNRTG	2 cut(s)	552, 1179
SmlI	CTYRAG	1 cut(s)	113
SmoI	CTYRAG	1 cut(s)	113
SphI	GCATGC	1 cut(s)	551
Sse9I	AATT	5 cut(s)	152, 906, 1005, 1062, 1074
SspI	AATATT	2 cut(s)	18, 24
SspMI	CTAG	3 cut(s)	197, 788, 932
SstI	GAGCTC	2 cut(s)	114, 120
StyD4I	CCNGG	4 cut(s)	57, 82, 422, 429
StyI	CCWWGG	3 cut(s)	835, 1197, 1320
TaaI	ACNGT	8 cut(s)	31, 55, 95, 125, 191, 634, 1114, 1156
TaiI	ACGT	2 cut(s)	221, 288
TaqI	TCGA	9 cut(s)	114, 120, 209, 351, 396, 770, 861, 1362, 1425
TasI	AATT	5 cut(s)	152, 906, 1005, 1062, 1074
TauI	GCSGC	5 cut(s)	183, 453, 884, 887, 1237
TfiI	GAWTC	1 cut(s)	251
Tru1I	TTAA	4 cut(s)	20, 155, 963, 1435
Tru9I	TTAA	4 cut(s)	20, 155, 963, 1435
TseFI	GTSAC	3 cut(s)	95, 571, 971
TseI	GCWGC	6 cut(s)	274, 590, 728, 875, 878, 1388
Tsp45I	GTSAC	3 cut(s)	95, 571, 971
TspDTI	ATGAA	1 cut(s)	1348
TspGWI	ACGGA	2 cut(s)	399, 940
VpaK11BI	GGWCC	2 cut(s)	779, 1211
VspI	ATTAAT	1 cut(s)	20
XagI	CCTNNNNNAGG	1 cut(s)	623
XbaI	TCTAGA	1 cut(s)	931
XceI	RCATGY	3 cut(s)	551, 572, 1184
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1204
XhoI	CTCGAG	1 cut(s)	113
XmiI	GTMKAC	1 cut(s)	861
XspI	CTAG	3 cut(s)	197, 788, 932
ZraI	GACGTC	1 cut(s)	219
Zsp2I	ATGCAT	1 cut(s)	549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.