Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0010157

Physical Location & Seq

Genomic Coordinates Forward (+)

56408018 .. 56411598

Gene Structure 3581 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G032270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGAATTCCAATAAAATAATACTATTTTCAGCACTAATCCTCACCTCTCTCATAGTCCCGGCGGAGCCGACGCCGTCCGCTCCTGGCGATCACAGCCGTCCGATTGCGGAGCTCGAAGTCGACTGTTGCCCCAACTGCAAGCCAAGCAGCCCCGAATTAGCCGCCACCAAAAAAAATTATAGCCGCCTCCACCTTGCCCTAGCGACTCCAACGACGACGTCTCCGCCGCCGTCGTGCGAGGTCTGGACGACGTCGTGCTCGGAATCGGTCCTGAGCTTCGCGAAGCAGCCGGAGAACGTGGAGTGGTTGAAGACGATTAGGCGGAGGATCCATGAGCATCCGGAGCTGGCTTTCGAGGAGGTCGAGACGAGTCGGTTGGTTCGGGCCGAGCTCGACCGCTTGGACATCGATTACGGTTTCCGCTCGCAACCACCGGGATTCGAGCCTGGATCGGAACCGGAGGAAGCTGTAGAATGGGAGCACAAGAGCAAAGTAGCCGGCAAAATGCATGCTTGTGGGCACGATGCACATGTGACCATGCTCTTAGGTGCAGCCAAGATCTTGAAGAGCCGTGAACACCTTTTGAAGGGGACTGTAGTATTGATATTTCAACCGGCAGAGGAAGCCGGTAATGGTGCAAAGAGAATGATTGGAGATGGGGCTTTGGAGAATGTGGAGGCCATATTCGCAGCTCATGTGTCCCACGAGCACCCCACAGGCATTATTGGGTCGAGGTCTGGTCCTTTGCTAGCTGGCTGTGGCTTCTTCAGAGCTGTTATCACCGGAAGGACCGGCCATGGCGAAAGCCCTCACCACTCTATTGACGCCGTTTTGGCAGCTGCCGCGACCGTGATCAGCTTACAGGGCATTGTATCGCGCGAAGCGAACCCTCTCGACTCTCAGGTGGTGTCCGTGACAGCTTTTAATGGAGGTGACAAGCTTGACATGATACCTAATAGTGTGGTAATTGGTGGGACTCTCAGGGCTTTCTCTAACACAAGCTTCTACCATCTTCTTCAAAGAATTGAAGAGGTAATTGTGGAACAAGCAAGTGTTTACAGATGCTCGGCAACGGTGGATTTCTTCAAAGACAAGAACACCATATATCCCCCGACGGTGAACGATGAGAAGATGCACGAGCACGTGAGGAAAGTAGCCATGGATTTGTTGGGTCCGACAAACTTTAGGTTGGTGCCACCAATGATGGGTGCTGAGGACTTCTCCTTCTACTCAGAGGTGATCCCTGCCGGCTTCTTCTACATTGGCATAAGGAACGAGACCTTGGGTTCCTTTCACACAGGTCATTCACCTTATTTTTTCATCGACGAAGATGTTTTACCGATAGGGGCAGCAACTCATGCCACTATCGCTGAAAGATACCTCAACGAGCGTGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0006082 GO:0008150 GO:0008152 GO:0009694 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0010112 GO:0010817 GO:0016787 GO:0016810 GO:0019752 GO:0031347 GO:0032101 GO:0032787 GO:0042221 GO:0042445 GO:0043436 GO:0043900 GO:0044237 GO:0044281 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0080134 GO:1901700 GO:1990206

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

465

Amino Acids

50.74

Weight (kDa)

5.71

Isoelectric Point (pI)

42.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	154 - 458	9.3e-40	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44350
fragaria_vesca	FvH4_2g27160 FvH4_2g27160
malus_domestica	MD08G1161700.v1.1 MD15G1346800.v1.1
prunus_persica	Prupe.1G494400_v2.0.a1 Prupe.1G494400_v2.0.a1
pyrus_communis	pycom08g13900 pycom15g31060
rosa_chinensis	RchiOBHm_Chr6g0296071
rosa_laevigata	RLG00000011736
rosa_multiflora	Rmu_sc0003139.1_g000031 Rmu_sc0004275.1_g000018
rosa_roxburghii	Rroxscaffold_7G00171850
rosa_rugosa	Rorug06G0256900 Rorug06G0257000
rosa_samantha	Rh6AG370300 Rh6BG377400 Rh6CG383300 Rh6DG370100
rosa_wichuraiana	Rw6G032270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	221, 254
AccB1I	GGYRCC	1 cut(s)	1192
AccBSI	CCGCTC	2 cut(s)	80, 423
AccI	GTMKAC	1 cut(s)	120
AccII	CGCG	4 cut(s)	281, 845, 877, 879
AccIII	TCCGGA	1 cut(s)	340
AclWI	GGATC	4 cut(s)	322, 335, 457, 1234
AcoI	YGGCCR	1 cut(s)	793
AcsI	RAATTY	1 cut(s)	4
AcuI	CTGAAG	1 cut(s)	751
AcvI	CACGTG	1 cut(s)	1144
AcyI	GRCGYC	4 cut(s)	71, 218, 251, 825
AfiI	CCNNNNNNNGG	2 cut(s)	586, 1205
AflIII	ACRYGT	1 cut(s)	529
AgsI	TTSAA	7 cut(s)	310, 565, 586, 611, 1019, 1028, 1087
AjnI	CCWGG	2 cut(s)	82, 445
AjuI	GAANNNNNNNTTGG	2 cut(s)	1265, 1297
Alw21I	GWGCWC	6 cut(s)	114, 260, 393, 483, 711, 1143
Alw26I	GTCTC	3 cut(s)	225, 359, 1271
AlwI	GGATC	4 cut(s)	322, 335, 457, 1234
Aor13HI	TCCGGA	1 cut(s)	340
AoxI	GGCC	3 cut(s)	384, 678, 793
ApeKI	GCWGC	7 cut(s)	147, 286, 551, 689, 836, 839, 1349
ApoI	RAATTY	1 cut(s)	4
AspLEI	GCGC	1 cut(s)	879
AspS9I	GGNCC	5 cut(s)	268, 384, 740, 789, 1172
AsuC2I	CCSGG	2 cut(s)	59, 435
AsuHPI	GGTGA	7 cut(s)	34, 772, 803, 944, 1129, 1249, 1299
AsuNHI	GCTAGC	1 cut(s)	748
AvaII	GGWCC	4 cut(s)	268, 740, 789, 1172
BaeGI	GKGCMC	1 cut(s)	522
BamHI	GGATCC	1 cut(s)	327
BanI	GGYRCC	1 cut(s)	1192
BanII	GRGCYC	2 cut(s)	114, 393
BarI	GAAGNNNNNNTAC	2 cut(s)	1320, 1352
BauI	CACGAG	2 cut(s)	704, 1136
BbrPI	CACGTG	1 cut(s)	1144
BbsI	GAAGAC	1 cut(s)	317
Bbv12I	GWGCWC	6 cut(s)	114, 260, 393, 483, 711, 1143
BbvCI	CCTCAGC	1 cut(s)	1212
BbvI	GCAGC	7 cut(s)	159, 298, 563, 701, 826, 848, 1361
BccI	CCATC	3 cut(s)	650, 1017, 1198
BceAI	ACGGC	5 cut(s)	58, 81, 214, 555, 812
BciT130I	CCWGG	2 cut(s)	84, 447
BclI	TGATCA	1 cut(s)	852
BcnI	CCSGG	2 cut(s)	59, 435
BcoDI	GTCTC	3 cut(s)	225, 359, 1271
BfaI	CTAG	2 cut(s)	200, 749
BfmI	CTRYAG	2 cut(s)	468, 594
BglI	GCCNNNNNGGC	1 cut(s)	833
BglII	AGATCT	1 cut(s)	558
Bme1390I	CCNGG	4 cut(s)	59, 84, 435, 447
Bme18I	GGWCC	4 cut(s)	268, 740, 789, 1172
BmgT120I	GGNCC	5 cut(s)	268, 384, 740, 789, 1172
BmiI	GGNNCC	6 cut(s)	66, 329, 456, 1173, 1194, 1288
BmrFI	CCNGG	4 cut(s)	59, 84, 435, 447
BmsI	GCATC	4 cut(s)	346, 514, 1052, 1122
BmtI	GCTAGC	1 cut(s)	752
BpiI	GAAGAC	1 cut(s)	317
BplI	GAGNNNNNCTC	2 cut(s)	1205, 1237
Bpu10I	CCTNAGC	2 cut(s)	272, 1212
BpuMI	CCSGG	2 cut(s)	59, 435
Bsa29I	ATCGAT	1 cut(s)	408
BsaAI	YACGTR	1 cut(s)	1144
BsaHI	GRCGYC	4 cut(s)	71, 218, 251, 825
BsaI	GGTCTC	1 cut(s)	1271
BsaJI	CCNNGG	3 cut(s)	796, 1158, 1281
BsaWI	WCCGGW	3 cut(s)	340, 457, 782
BsaXI	ACNNNNNCTCC	2 cut(s)	205, 235
Bsc4I	CCNNNNNNNGG	2 cut(s)	586, 1205
Bse118I	RCCGGY	5 cut(s)	497, 613, 626, 791, 1247
BseAI	TCCGGA	1 cut(s)	340
BseBI	CCWGG	2 cut(s)	84, 447
BseCI	ATCGAT	1 cut(s)	408
BseDI	CCNNGG	3 cut(s)	796, 1158, 1281
BseGI	GGATG	1 cut(s)	337
BseLI	CCNNNNNNNGG	2 cut(s)	586, 1205
BseMII	CTCAG	5 cut(s)	263, 914, 994, 1203, 1245
BseRI	GAGGAG	1 cut(s)	371
BseSI	GKGCMC	1 cut(s)	522
BseXI	GCAGC	7 cut(s)	159, 298, 563, 701, 826, 848, 1361
BsgI	GTGCAG	1 cut(s)	570
Bsh1236I	CGCG	4 cut(s)	281, 845, 877, 879
Bsh1285I	CGRYCG	2 cut(s)	397, 849
BshFI	GGCC	3 cut(s)	386, 680, 795
BshNI	GGYRCC	1 cut(s)	1192
BshVI	ATCGAT	1 cut(s)	408
BsiEI	CGRYCG	2 cut(s)	397, 849
BsiHKAI	GWGCWC	6 cut(s)	114, 260, 393, 483, 711, 1143
BslFI	GGGAC	4 cut(s)	41, 604, 685, 988
BslI	CCNNNNNNNGG	2 cut(s)	586, 1205
BsmAI	GTCTC	3 cut(s)	225, 359, 1271
BsmBI	CGTCTC	2 cut(s)	225, 359
BsmFI	GGGAC	4 cut(s)	41, 604, 685, 988
BsnI	GGCC	3 cut(s)	386, 680, 795
Bso31I	GGTCTC	1 cut(s)	1271
Bsp1286I	GDGCHC	7 cut(s)	114, 260, 393, 483, 522, 711, 1143
Bsp13I	TCCGGA	1 cut(s)	340
Bsp143I	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
Bsp19I	CCATGG	2 cut(s)	796, 1158
Bsp68I	TCGCGA	1 cut(s)	281
BspANI	GGCC	3 cut(s)	386, 680, 795
BspCNI	CTCAG	5 cut(s)	264, 913, 993, 1204, 1244
BspDI	ATCGAT	1 cut(s)	408
BspEI	TCCGGA	1 cut(s)	340
BspFNI	CGCG	4 cut(s)	281, 845, 877, 879
BspLI	GGNNCC	6 cut(s)	66, 329, 456, 1173, 1194, 1288
BspOI	GCTAGC	1 cut(s)	752
BspPI	GGATC	4 cut(s)	322, 335, 457, 1234
BspQI	GCTCTTC	1 cut(s)	560
BspT107I	GGYRCC	1 cut(s)	1192
BspTNI	GGTCTC	1 cut(s)	1271
BsrBI	CCGCTC	2 cut(s)	80, 423
BsrFI	RCCGGY	5 cut(s)	497, 613, 626, 791, 1247
BssAI	RCCGGY	5 cut(s)	497, 613, 626, 791, 1247
BssECI	CCNNGG	3 cut(s)	796, 1158, 1281
BssMI	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
BssNI	GRCGYC	4 cut(s)	71, 218, 251, 825
BssSI	CACGAG	2 cut(s)	704, 1136
BssT1I	CCWWGG	3 cut(s)	796, 1158, 1281
Bst2BI	CACGAG	2 cut(s)	704, 1136
Bst2UI	CCWGG	2 cut(s)	84, 447
Bst4CI	ACNGT	6 cut(s)	125, 416, 595, 850, 1075, 1117
Bst6I	CTCTTC	2 cut(s)	560, 1023
BstACI	GRCGYC	4 cut(s)	71, 218, 251, 825
BstAPI	GCANNNNNTGC	1 cut(s)	1358
BstBAI	YACGTR	1 cut(s)	1144
BstC8I	GCNNGC	8 cut(s)	140, 348, 425, 499, 510, 750, 754, 1249
BstDEI	CTNAG	6 cut(s)	272, 544, 900, 980, 1212, 1231
BstDSI	CCRYGG	2 cut(s)	796, 1158
BstENI	CCTNNNNNAGG	1 cut(s)	584
BstF5I	GGATG	1 cut(s)	337
BstFNI	CGCG	4 cut(s)	281, 845, 877, 879
BstHHI	GCGC	1 cut(s)	879
BstKTI	GATC	6 cut(s)	91, 330, 452, 561, 855, 1242
BstMAI	GTCTC	3 cut(s)	225, 359, 1271
BstMBI	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
BstMCI	CGRYCG	2 cut(s)	397, 849
BstNI	CCWGG	2 cut(s)	84, 447
BstNSI	RCATGY	2 cut(s)	512, 533
BstSCI	CCNGG	4 cut(s)	57, 82, 433, 445
BstSFI	CTRYAG	2 cut(s)	468, 594
BstSLI	GKGCMC	1 cut(s)	522
BstUI	CGCG	4 cut(s)	281, 845, 877, 879
BstV1I	GCAGC	7 cut(s)	159, 298, 563, 701, 826, 848, 1361
BstV2I	GAAGAC	1 cut(s)	317
BstX2I	RGATCY	2 cut(s)	327, 558
BstYI	RGATCY	2 cut(s)	327, 558
Bsu15I	ATCGAT	1 cut(s)	408
BsuRI	GGCC	3 cut(s)	386, 680, 795
BsuTUI	ATCGAT	1 cut(s)	408
BtgI	CCRYGG	2 cut(s)	796, 1158
BtsCI	GGATG	1 cut(s)	337
BtuMI	TCGCGA	1 cut(s)	281
Cac8I	GCNNGC	8 cut(s)	140, 348, 425, 499, 510, 750, 754, 1249
CfoI	GCGC	1 cut(s)	879
Cfr10I	RCCGGY	5 cut(s)	497, 613, 626, 791, 1247
Cfr13I	GGNCC	5 cut(s)	268, 384, 740, 789, 1172
ClaI	ATCGAT	1 cut(s)	408
CseI	GACGC	2 cut(s)	79, 833
CspCI	CAANNNNNGTGG	1 cut(s)	1372
CviAII	CATG	9 cut(s)	332, 509, 530, 538, 695, 797, 946, 1159, 1358
DdeI	CTNAG	6 cut(s)	272, 544, 900, 980, 1212, 1231
DpnI	GATC	6 cut(s)	90, 329, 451, 560, 854, 1241
DpnII	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
EaeI	YGGCCR	1 cut(s)	793
Eam1104I	CTCTTC	2 cut(s)	560, 1023
EarI	CTCTTC	2 cut(s)	560, 1023
EciI	GGCGGA	3 cut(s)	77, 213, 337
Ecl136II	GAGCTC	2 cut(s)	112, 391
Eco130I	CCWWGG	3 cut(s)	796, 1158, 1281
Eco24I	GRGCYC	2 cut(s)	114, 393
Eco31I	GGTCTC	1 cut(s)	1271
Eco47I	GGWCC	4 cut(s)	268, 740, 789, 1172
Eco53kI	GAGCTC	2 cut(s)	112, 391
Eco57I	CTGAAG	1 cut(s)	751
Eco72I	CACGTG	1 cut(s)	1144
EcoICRI	GAGCTC	2 cut(s)	112, 391
EcoNI	CCTNNNNNAGG	1 cut(s)	584
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	2 cut(s)	82, 445
EcoT14I	CCWWGG	3 cut(s)	796, 1158, 1281
EcoT22I	ATGCAT	1 cut(s)	510
EcoT38I	GRGCYC	2 cut(s)	114, 393
ErhI	CCWWGG	3 cut(s)	796, 1158, 1281
Esp3I	CGTCTC	2 cut(s)	225, 359
FaeI	CATG	9 cut(s)	335, 512, 533, 541, 698, 800, 949, 1162, 1361
FaqI	GGGAC	4 cut(s)	41, 604, 685, 988
FatI	CATG	9 cut(s)	331, 508, 529, 537, 694, 796, 945, 1158, 1357
FbaI	TGATCA	1 cut(s)	852
FblI	GTMKAC	1 cut(s)	120
FokI	GGATG	1 cut(s)	324
FriOI	GRGCYC	2 cut(s)	114, 393
FspBI	CTAG	2 cut(s)	200, 749
GlaI	GCGC	1 cut(s)	878
HaeIII	GGCC	3 cut(s)	386, 680, 795
HgaI	GACGC	2 cut(s)	79, 833
HhaI	GCGC	1 cut(s)	879
Hin1I	GRCGYC	4 cut(s)	71, 218, 251, 825
Hin1II	CATG	9 cut(s)	335, 512, 533, 541, 698, 800, 949, 1162, 1361
Hin6I	GCGC	1 cut(s)	877
HinP1I	GCGC	1 cut(s)	877
HincII	GTYRAC	1 cut(s)	121
HindII	GTYRAC	1 cut(s)	121
HindIII	AAGCTT	2 cut(s)	938, 1000
HinfI	GANTC	6 cut(s)	205, 263, 370, 438, 896, 976
HphI	GGTGA	7 cut(s)	34, 772, 803, 944, 1129, 1249, 1299
Hpy166II	GTNNAC	4 cut(s)	121, 575, 1057, 1120
Hpy188I	TCNGA	6 cut(s)	102, 262, 454, 770, 1176, 1234
Hpy188III	TCNNGA	7 cut(s)	244, 271, 280, 341, 364, 562, 893
Hpy8I	GTNNAC	4 cut(s)	121, 575, 1057, 1120
Hpy99I	CGWCG	8 cut(s)	73, 217, 220, 235, 253, 256, 1117, 1328
HpyAV	CCTTC	3 cut(s)	580, 780, 1234
HpyCH4III	ACNGT	6 cut(s)	125, 416, 595, 850, 1075, 1117
HpyCH4IV	ACGT	4 cut(s)	218, 251, 297, 1143
HpyCH4V	TGCA	6 cut(s)	138, 508, 527, 551, 638, 1135
HpyF3I	CTNAG	6 cut(s)	272, 544, 900, 980, 1212, 1231
HpySE526I	ACGT	4 cut(s)	218, 251, 297, 1143
Hsp92I	GRCGYC	4 cut(s)	71, 218, 251, 825
Hsp92II	CATG	9 cut(s)	335, 512, 533, 541, 698, 800, 949, 1162, 1361
HspAI	GCGC	1 cut(s)	877
Kpn2I	TCCGGA	1 cut(s)	340
KroI	GCCGGC	2 cut(s)	497, 1247
KroNI	GCCGGC	2 cut(s)	499, 1249
Ksp22I	TGATCA	1 cut(s)	852
Kzo9I	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
LguI	GCTCTTC	1 cut(s)	560
LmnI	GCTCC	5 cut(s)	64, 85, 109, 343, 478
Lsp1109I	GCAGC	7 cut(s)	159, 298, 563, 701, 826, 848, 1361
LweI	GCATC	4 cut(s)	346, 514, 1052, 1122
MaeI	CTAG	2 cut(s)	200, 749
MaeII	ACGT	4 cut(s)	218, 251, 297, 1143
MaeIII	GTNAC	3 cut(s)	532, 913, 932
MalI	GATC	6 cut(s)	90, 329, 451, 560, 854, 1241
MbiI	CCGCTC	2 cut(s)	80, 423
MboI	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
MflI	RGATCY	2 cut(s)	327, 558
MhlI	GDGCHC	7 cut(s)	114, 260, 393, 483, 522, 711, 1143
MluCI	AATT	6 cut(s)	4, 155, 175, 966, 1023, 1035
MlyI	GAGTC	4 cut(s)	199, 379, 890, 970
MmeI	TCCRAC	2 cut(s)	233, 1199
Mph1103I	ATGCAT	1 cut(s)	510
MroI	TCCGGA	1 cut(s)	340
MroNI	GCCGGC	2 cut(s)	497, 1247
MseI	TTAA	1 cut(s)	924
MslI	CAYNNNNRTG	1 cut(s)	513
MspA1I	CMGCKG	1 cut(s)	839
MspR9I	CCNGG	4 cut(s)	59, 84, 435, 447
MvaI	CCWGG	2 cut(s)	84, 447
MvnI	CGCG	4 cut(s)	281, 845, 877, 879
NaeI	GCCGGC	2 cut(s)	499, 1249
NciI	CCSGG	2 cut(s)	59, 435
NcoI	CCATGG	2 cut(s)	796, 1158
NdeII	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
NgoMIV	GCCGGC	2 cut(s)	497, 1247
NheI	GCTAGC	1 cut(s)	748
NlaIII	CATG	9 cut(s)	335, 512, 533, 541, 698, 800, 949, 1162, 1361
NlaIV	GGNNCC	6 cut(s)	66, 329, 456, 1173, 1194, 1288
NmeAIII	GCCGAG	2 cut(s)	412, 1046
NmuCI	GTSAC	3 cut(s)	532, 913, 932
NruI	TCGCGA	1 cut(s)	281
NsiI	ATGCAT	1 cut(s)	510
NspI	RCATGY	2 cut(s)	512, 533
PaeI	GCATGC	1 cut(s)	512
PciI	ACATGT	1 cut(s)	529
PciSI	GCTCTTC	1 cut(s)	560
PcsI	WCGNNNNNNNCGW	2 cut(s)	360, 881
PdiI	GCCGGC	2 cut(s)	499, 1249
PfeI	GAWTC	2 cut(s)	263, 438
PflFI	GACNNNGTC	3 cut(s)	73, 217, 250
PleI	GAGTC	4 cut(s)	199, 378, 890, 970
PmaCI	CACGTG	1 cut(s)	1144
PmlI	CACGTG	1 cut(s)	1144
PpsI	GAGTC	4 cut(s)	199, 378, 890, 970
Ppu21I	YACGTR	1 cut(s)	1144
PscI	ACATGT	1 cut(s)	529
Psp124BI	GAGCTC	2 cut(s)	114, 393
Psp6I	CCWGG	2 cut(s)	82, 445
PspCI	CACGTG	1 cut(s)	1144
PspGI	CCWGG	2 cut(s)	82, 445
PspN4I	GGNNCC	6 cut(s)	66, 329, 456, 1173, 1194, 1288
PspPI	GGNCC	5 cut(s)	268, 384, 740, 789, 1172
PsuI	RGATCY	2 cut(s)	327, 558
PsyI	GACNNNGTC	3 cut(s)	73, 217, 250
PvuII	CAGCTG	1 cut(s)	839
RruI	TCGCGA	1 cut(s)	281
RseI	CAYNNNNRTG	1 cut(s)	513
SacI	GAGCTC	2 cut(s)	114, 393
SalI	GTCGAC	1 cut(s)	119
SapI	GCTCTTC	1 cut(s)	560
SaqAI	TTAA	1 cut(s)	924
Sau3AI	GATC	6 cut(s)	88, 327, 449, 558, 852, 1239
Sau96I	GGNCC	5 cut(s)	268, 384, 740, 789, 1172
SchI	GAGTC	4 cut(s)	199, 379, 890, 970
ScrFI	CCNGG	4 cut(s)	59, 84, 435, 447
SduI	GDGCHC	7 cut(s)	114, 260, 393, 483, 522, 711, 1143
SfaNI	GCATC	4 cut(s)	346, 514, 1052, 1122
SfcI	CTRYAG	2 cut(s)	468, 594
SinI	GGWCC	4 cut(s)	268, 740, 789, 1172
SmiMI	CAYNNNNRTG	1 cut(s)	513
SphI	GCATGC	1 cut(s)	512
Sse9I	AATT	6 cut(s)	4, 155, 175, 966, 1023, 1035
SspMI	CTAG	2 cut(s)	200, 749
SstI	GAGCTC	2 cut(s)	114, 393
StyD4I	CCNGG	4 cut(s)	57, 82, 433, 445
StyI	CCWWGG	3 cut(s)	796, 1158, 1281
TaaI	ACNGT	6 cut(s)	125, 416, 595, 850, 1075, 1117
TaiI	ACGT	4 cut(s)	221, 254, 300, 1146
TaqII	GACCGA	1 cut(s)	256
TasI	AATT	6 cut(s)	4, 155, 175, 966, 1023, 1035
TauI	GCSGC	4 cut(s)	164, 186, 229, 845
TfiI	GAWTC	2 cut(s)	263, 438
Tru1I	TTAA	1 cut(s)	924
Tru9I	TTAA	1 cut(s)	924
TseFI	GTSAC	3 cut(s)	532, 913, 932
TseI	GCWGC	7 cut(s)	147, 286, 551, 689, 836, 839, 1349
Tsp45I	GTSAC	3 cut(s)	532, 913, 932
TspDTI	ATGAA	2 cut(s)	17, 1309
TspGWI	ACGGA	1 cut(s)	901
Tth111I	GACNNNGTC	3 cut(s)	73, 217, 250
VpaK11BI	GGWCC	4 cut(s)	268, 740, 789, 1172
XagI	CCTNNNNNAGG	1 cut(s)	584
XapI	RAATTY	1 cut(s)	4
XceI	RCATGY	2 cut(s)	512, 533
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1165
XmiI	GTMKAC	1 cut(s)	120
XspI	CTAG	2 cut(s)	200, 749
ZraI	GACGTC	2 cut(s)	219, 252
Zsp2I	ATGCAT	1 cut(s)	510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.