Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0010157

Physical Location & Seq

Genomic Coordinates Forward (+)

56854609 .. 56858678

Gene Structure 4070 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG370300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGAATTCCAATAAAATAATACTATTTTCAGCACTAATCCTCACCTCTCTCATAGTCCCGGCGGAGCCGACGCCGTCCGCTCCTGGCGATCACAGCCGTCCGATTGCGGAGCTCGAAGTCGACTGTTGCCCCAACTGCAAGCCAAGCAGCCCCGAATTAGCCGCCACCAAAAAAAATTATAGCCGCCTCCACCTTGCCCTAGCGACTCCAACGACGACGTCTCCGCTGCCGTCGTGCGAGGTCTGGACGACGTCGTGCTCGGAATCTTTCCTGAGCTTCGCGAAGCAGCCGGAGAACGTGGAGTGGTTGAAGACGATTAGGCGGAGGATCCATGAGCATCCGGAGCTGGCTTTCGAGGAGGTCGAGACGAGTCGGTTGGTTCGGGCCGAGCTCGACCGCTTGGACATCGATTACCGGTTTCCGCTCGCAACCACCGGGATTCGAGCCTGGATCGGAACCGGAGGTCCGCCGTTTGTGGCTGTCAGAGCTGACATGGATGCTCTACCAATTCAGGAAGCTGTAGAATGGGAGCACAAGAGCAAAGTAGCCGGCAAAATGCATGCTTGTGGGCACGATGCACATGTGACCATGCTCTTAGGTGCAGCCAAGATCTTGAAGAGCCGTGAACACCTTTTGAAGGGGACTGTAGTATTGATATTTCAACCGGCAGAGGAAGCCGGTAATGGTGCAAAGAGAATGATTGGAGATGGGGCTTTGGAGAATGTGGAGGCCATATTCGCAGCTCATGTGTCCCACGAGCACCCCACAGGCATTATTGGGTCGAGGTCTGGTCCTTTGCTAGCTGGCTGTGGCTTCTTCAGAGCTGTTATCACCGGAAGGACCGGCCATGGCGAAAGCCCTCACCACTCTATTGACGCCGTTTTGGCAGCTGCCGCGACCGTGATCAGCTTACAGGGCATTGTATCGCGCGAAGCGAACCCTCTCGACTCTCAGGTGGTGTCCGTGACAGCTTTTAATGGAGGTGACAAGCTTGACATGATACCTAATAGTGTGGTAATTGGTGGGACTCTCAGGGCTTTCTCTAACACTAGCTTCTACCATCTTCTTCAAAGAATTGAAGAGGTAATTATGGAACAAGCAAGTGTTTACAGATGCTCGGCAACGGTGGATTTCTTCAAAGACAAGAACACCATATATCCCCCGACGGTGAACGATGAGAAGATGCACGAGCACGTGAGGAAAGTAGCCATGGATTTGTTGGGTCCGACAAACTTTAGGTTGGTGCCACCAATGATGGGTGCTGAGGACTTCTCCTTCTACTCAGAGGTGATCCCTGCCGGCTTCTTCTACATTGGCATAAGGAACGAGACCTTGGGTTCCTTTCACACAGGTCATTCACCTTATTTTTTCATCGACGAAGATGTTTTACCGATAGGGGCAGCAACTCATGCCACAATCGCTGAAAGATACCTCAACGAGCGTGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0006082 GO:0008150 GO:0008152 GO:0009694 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0010112 GO:0010817 GO:0016787 GO:0016810 GO:0019752 GO:0031347 GO:0032101 GO:0032787 GO:0042221 GO:0042445 GO:0043436 GO:0043900 GO:0044237 GO:0044281 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0080134 GO:1901700 GO:1990206

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

482

Amino Acids

52.61

Weight (kDa)

5.89

Isoelectric Point (pI)

37.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	160 - 475	8e-45	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44350
fragaria_vesca	FvH4_2g27160 FvH4_2g27160
malus_domestica	MD08G1161700.v1.1 MD15G1346800.v1.1
prunus_persica	Prupe.1G494400_v2.0.a1 Prupe.1G494400_v2.0.a1
pyrus_communis	pycom08g13900 pycom15g31060
rosa_chinensis	RchiOBHm_Chr6g0296071
rosa_laevigata	RLG00000011736
rosa_multiflora	Rmu_sc0003139.1_g000031 Rmu_sc0004275.1_g000018
rosa_roxburghii	Rroxscaffold_7G00171850
rosa_rugosa	Rorug06G0256900 Rorug06G0257000
rosa_samantha	Rh6AG370300 Rh6BG377400 Rh6CG383300 Rh6DG370100
rosa_wichuraiana	Rw6G032270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	221, 254
AccB1I	GGYRCC	1 cut(s)	1243
AccBSI	CCGCTC	2 cut(s)	80, 424
AccI	GTMKAC	1 cut(s)	120
AccII	CGCG	4 cut(s)	281, 896, 928, 930
AccIII	TCCGGA	1 cut(s)	340
AclWI	GGATC	4 cut(s)	322, 335, 458, 1285
AcoI	YGGCCR	1 cut(s)	844
AcsI	RAATTY	1 cut(s)	4
AcuI	CTGAAG	1 cut(s)	802
AcvI	CACGTG	1 cut(s)	1195
AcyI	GRCGYC	4 cut(s)	71, 218, 251, 876
AfiI	CCNNNNNNNGG	2 cut(s)	637, 1256
AflIII	ACRYGT	1 cut(s)	580
AgeI	ACCGGT	1 cut(s)	414
AgsI	TTSAA	7 cut(s)	310, 616, 637, 662, 1070, 1079, 1138
AjnI	CCWGG	2 cut(s)	82, 446
AjuI	GAANNNNNNNTTGG	2 cut(s)	1316, 1348
AloI	GAACNNNNNNTCC	2 cut(s)	448, 480
Alw21I	GWGCWC	6 cut(s)	114, 260, 393, 534, 762, 1194
Alw26I	GTCTC	3 cut(s)	225, 359, 1322
AlwI	GGATC	4 cut(s)	322, 335, 458, 1285
Aor13HI	TCCGGA	1 cut(s)	340
AoxI	GGCC	3 cut(s)	384, 729, 844
ApeKI	GCWGC	8 cut(s)	147, 226, 286, 602, 740, 887, 890, 1400
ApoI	RAATTY	1 cut(s)	4
AsiGI	ACCGGT	1 cut(s)	414
AspLEI	GCGC	1 cut(s)	930
AspS9I	GGNCC	5 cut(s)	384, 464, 791, 840, 1223
AsuC2I	CCSGG	2 cut(s)	59, 436
AsuHPI	GGTGA	7 cut(s)	34, 823, 854, 995, 1180, 1300, 1350
AsuNHI	GCTAGC	1 cut(s)	799
AvaII	GGWCC	4 cut(s)	464, 791, 840, 1223
BaeGI	GKGCMC	1 cut(s)	573
BamHI	GGATCC	1 cut(s)	327
BanI	GGYRCC	1 cut(s)	1243
BanII	GRGCYC	2 cut(s)	114, 393
BarI	GAAGNNNNNNTAC	2 cut(s)	1371, 1403
BauI	CACGAG	2 cut(s)	755, 1187
BbrPI	CACGTG	1 cut(s)	1195
BbsI	GAAGAC	1 cut(s)	317
Bbv12I	GWGCWC	6 cut(s)	114, 260, 393, 534, 762, 1194
BbvCI	CCTCAGC	1 cut(s)	1263
BbvI	GCAGC	8 cut(s)	159, 213, 298, 614, 752, 877, 899, 1412
BccI	CCATC	3 cut(s)	701, 1068, 1249
BceAI	ACGGC	6 cut(s)	58, 81, 214, 454, 606, 863
BciT130I	CCWGG	2 cut(s)	84, 448
BclI	TGATCA	1 cut(s)	903
BcnI	CCSGG	2 cut(s)	59, 436
BcoDI	GTCTC	3 cut(s)	225, 359, 1322
BfaI	CTAG	3 cut(s)	200, 800, 1050
BfmI	CTRYAG	2 cut(s)	519, 645
BglI	GCCNNNNNGGC	1 cut(s)	884
BglII	AGATCT	1 cut(s)	609
Bme1390I	CCNGG	4 cut(s)	59, 84, 436, 448
Bme18I	GGWCC	4 cut(s)	464, 791, 840, 1223
BmgT120I	GGNCC	5 cut(s)	384, 464, 791, 840, 1223
BmiI	GGNNCC	6 cut(s)	66, 329, 457, 1224, 1245, 1339
BmrFI	CCNGG	4 cut(s)	59, 84, 436, 448
BmsI	GCATC	5 cut(s)	346, 487, 565, 1103, 1173
BmtI	GCTAGC	1 cut(s)	803
BpiI	GAAGAC	1 cut(s)	317
BplI	GAGNNNNNCTC	2 cut(s)	1256, 1288
Bpu10I	CCTNAGC	2 cut(s)	272, 1263
BpuMI	CCSGG	2 cut(s)	59, 436
Bsa29I	ATCGAT	1 cut(s)	408
BsaAI	YACGTR	1 cut(s)	1195
BsaHI	GRCGYC	4 cut(s)	71, 218, 251, 876
BsaI	GGTCTC	1 cut(s)	1322
BsaJI	CCNNGG	3 cut(s)	847, 1209, 1332
BsaWI	WCCGGW	4 cut(s)	340, 414, 458, 833
BsaXI	ACNNNNNCTCC	2 cut(s)	205, 235
Bsc4I	CCNNNNNNNGG	2 cut(s)	637, 1256
Bse118I	RCCGGY	6 cut(s)	414, 548, 664, 677, 842, 1298
BseAI	TCCGGA	1 cut(s)	340
BseBI	CCWGG	2 cut(s)	84, 448
BseCI	ATCGAT	1 cut(s)	408
BseDI	CCNNGG	3 cut(s)	847, 1209, 1332
BseGI	GGATG	2 cut(s)	337, 502
BseLI	CCNNNNNNNGG	2 cut(s)	637, 1256
BseMII	CTCAG	5 cut(s)	263, 965, 1045, 1254, 1296
BseRI	GAGGAG	1 cut(s)	371
BseSI	GKGCMC	1 cut(s)	573
BseXI	GCAGC	8 cut(s)	159, 213, 298, 614, 752, 877, 899, 1412
BsgI	GTGCAG	1 cut(s)	621
Bsh1236I	CGCG	4 cut(s)	281, 896, 928, 930
Bsh1285I	CGRYCG	2 cut(s)	397, 900
BshFI	GGCC	3 cut(s)	386, 731, 846
BshNI	GGYRCC	1 cut(s)	1243
BshTI	ACCGGT	1 cut(s)	414
BshVI	ATCGAT	1 cut(s)	408
BsiEI	CGRYCG	2 cut(s)	397, 900
BsiHKAI	GWGCWC	6 cut(s)	114, 260, 393, 534, 762, 1194
BslFI	GGGAC	4 cut(s)	41, 655, 736, 1039
BslI	CCNNNNNNNGG	2 cut(s)	637, 1256
BsmAI	GTCTC	3 cut(s)	225, 359, 1322
BsmBI	CGTCTC	2 cut(s)	225, 359
BsmFI	GGGAC	4 cut(s)	41, 655, 736, 1039
BsnI	GGCC	3 cut(s)	386, 731, 846
Bso31I	GGTCTC	1 cut(s)	1322
Bsp1286I	GDGCHC	7 cut(s)	114, 260, 393, 534, 573, 762, 1194
Bsp13I	TCCGGA	1 cut(s)	340
Bsp143I	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
Bsp19I	CCATGG	2 cut(s)	847, 1209
Bsp68I	TCGCGA	1 cut(s)	281
BspANI	GGCC	3 cut(s)	386, 731, 846
BspCNI	CTCAG	5 cut(s)	264, 964, 1044, 1255, 1295
BspDI	ATCGAT	1 cut(s)	408
BspEI	TCCGGA	1 cut(s)	340
BspFNI	CGCG	4 cut(s)	281, 896, 928, 930
BspLI	GGNNCC	6 cut(s)	66, 329, 457, 1224, 1245, 1339
BspOI	GCTAGC	1 cut(s)	803
BspPI	GGATC	4 cut(s)	322, 335, 458, 1285
BspQI	GCTCTTC	1 cut(s)	611
BspT107I	GGYRCC	1 cut(s)	1243
BspTNI	GGTCTC	1 cut(s)	1322
BsrBI	CCGCTC	2 cut(s)	80, 424
BsrFI	RCCGGY	6 cut(s)	414, 548, 664, 677, 842, 1298
BssAI	RCCGGY	6 cut(s)	414, 548, 664, 677, 842, 1298
BssECI	CCNNGG	3 cut(s)	847, 1209, 1332
BssMI	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
BssNI	GRCGYC	4 cut(s)	71, 218, 251, 876
BssSI	CACGAG	2 cut(s)	755, 1187
BssT1I	CCWWGG	3 cut(s)	847, 1209, 1332
Bst2BI	CACGAG	2 cut(s)	755, 1187
Bst2UI	CCWGG	2 cut(s)	84, 448
Bst4CI	ACNGT	5 cut(s)	125, 646, 901, 1126, 1168
Bst6I	CTCTTC	2 cut(s)	611, 1074
BstACI	GRCGYC	4 cut(s)	71, 218, 251, 876
BstAPI	GCANNNNNTGC	1 cut(s)	1409
BstBAI	YACGTR	1 cut(s)	1195
BstC8I	GCNNGC	8 cut(s)	140, 348, 426, 550, 561, 801, 805, 1300
BstDEI	CTNAG	6 cut(s)	272, 595, 951, 1031, 1263, 1282
BstDSI	CCRYGG	2 cut(s)	847, 1209
BstENI	CCTNNNNNAGG	1 cut(s)	635
BstF5I	GGATG	2 cut(s)	337, 502
BstFNI	CGCG	4 cut(s)	281, 896, 928, 930
BstHHI	GCGC	1 cut(s)	930
BstKTI	GATC	6 cut(s)	91, 330, 453, 612, 906, 1293
BstMAI	GTCTC	3 cut(s)	225, 359, 1322
BstMBI	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
BstMCI	CGRYCG	2 cut(s)	397, 900
BstNI	CCWGG	2 cut(s)	84, 448
BstNSI	RCATGY	2 cut(s)	563, 584
BstSCI	CCNGG	4 cut(s)	57, 82, 434, 446
BstSFI	CTRYAG	2 cut(s)	519, 645
BstSLI	GKGCMC	1 cut(s)	573
BstUI	CGCG	4 cut(s)	281, 896, 928, 930
BstV1I	GCAGC	8 cut(s)	159, 213, 298, 614, 752, 877, 899, 1412
BstV2I	GAAGAC	1 cut(s)	317
BstX2I	RGATCY	2 cut(s)	327, 609
BstYI	RGATCY	2 cut(s)	327, 609
Bsu15I	ATCGAT	1 cut(s)	408
BsuRI	GGCC	3 cut(s)	386, 731, 846
BsuTUI	ATCGAT	1 cut(s)	408
BtgI	CCRYGG	2 cut(s)	847, 1209
BtsCI	GGATG	2 cut(s)	337, 502
BtuMI	TCGCGA	1 cut(s)	281
Cac8I	GCNNGC	8 cut(s)	140, 348, 426, 550, 561, 801, 805, 1300
CfoI	GCGC	1 cut(s)	930
Cfr10I	RCCGGY	6 cut(s)	414, 548, 664, 677, 842, 1298
Cfr13I	GGNCC	5 cut(s)	384, 464, 791, 840, 1223
ClaI	ATCGAT	1 cut(s)	408
CseI	GACGC	2 cut(s)	79, 884
CspAI	ACCGGT	1 cut(s)	414
CspCI	CAANNNNNGTGG	1 cut(s)	1423
DdeI	CTNAG	6 cut(s)	272, 595, 951, 1031, 1263, 1282
DpnI	GATC	6 cut(s)	90, 329, 452, 611, 905, 1292
DpnII	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
EaeI	YGGCCR	1 cut(s)	844
Eam1104I	CTCTTC	2 cut(s)	611, 1074
EarI	CTCTTC	2 cut(s)	611, 1074
EciI	GGCGGA	3 cut(s)	77, 337, 456
Ecl136II	GAGCTC	2 cut(s)	112, 391
Eco130I	CCWWGG	3 cut(s)	847, 1209, 1332
Eco24I	GRGCYC	2 cut(s)	114, 393
Eco31I	GGTCTC	1 cut(s)	1322
Eco47I	GGWCC	4 cut(s)	464, 791, 840, 1223
Eco53kI	GAGCTC	2 cut(s)	112, 391
Eco57I	CTGAAG	1 cut(s)	802
Eco72I	CACGTG	1 cut(s)	1195
EcoICRI	GAGCTC	2 cut(s)	112, 391
EcoNI	CCTNNNNNAGG	1 cut(s)	635
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	2 cut(s)	82, 446
EcoT14I	CCWWGG	3 cut(s)	847, 1209, 1332
EcoT22I	ATGCAT	1 cut(s)	561
EcoT38I	GRGCYC	2 cut(s)	114, 393
ErhI	CCWWGG	3 cut(s)	847, 1209, 1332
Esp3I	CGTCTC	2 cut(s)	225, 359
FaqI	GGGAC	4 cut(s)	41, 655, 736, 1039
FbaI	TGATCA	1 cut(s)	903
FblI	GTMKAC	1 cut(s)	120
FokI	GGATG	2 cut(s)	324, 509
FriOI	GRGCYC	2 cut(s)	114, 393
FspBI	CTAG	3 cut(s)	200, 800, 1050
GlaI	GCGC	1 cut(s)	929
HaeIII	GGCC	3 cut(s)	386, 731, 846
HgaI	GACGC	2 cut(s)	79, 884
HhaI	GCGC	1 cut(s)	930
Hin1I	GRCGYC	4 cut(s)	71, 218, 251, 876
Hin6I	GCGC	1 cut(s)	928
HinP1I	GCGC	1 cut(s)	928
HincII	GTYRAC	1 cut(s)	121
HindII	GTYRAC	1 cut(s)	121
HindIII	AAGCTT	1 cut(s)	989
HinfI	GANTC	6 cut(s)	205, 263, 370, 439, 947, 1027
HphI	GGTGA	7 cut(s)	34, 823, 854, 995, 1180, 1300, 1350
Hpy166II	GTNNAC	4 cut(s)	121, 626, 1108, 1171
Hpy188I	TCNGA	7 cut(s)	102, 262, 455, 485, 821, 1227, 1285
Hpy188III	TCNNGA	8 cut(s)	244, 271, 280, 341, 364, 512, 613, 944
Hpy8I	GTNNAC	4 cut(s)	121, 626, 1108, 1171
Hpy99I	CGWCG	8 cut(s)	73, 217, 220, 235, 253, 256, 1168, 1379
HpyAV	CCTTC	3 cut(s)	631, 831, 1285
HpyCH4III	ACNGT	5 cut(s)	125, 646, 901, 1126, 1168
HpyCH4IV	ACGT	4 cut(s)	218, 251, 297, 1194
HpyCH4V	TGCA	6 cut(s)	138, 559, 578, 602, 689, 1186
HpyF3I	CTNAG	6 cut(s)	272, 595, 951, 1031, 1263, 1282
HpySE526I	ACGT	4 cut(s)	218, 251, 297, 1194
Hsp92I	GRCGYC	4 cut(s)	71, 218, 251, 876
HspAI	GCGC	1 cut(s)	928
Kpn2I	TCCGGA	1 cut(s)	340
KroI	GCCGGC	2 cut(s)	548, 1298
KroNI	GCCGGC	2 cut(s)	550, 1300
Ksp22I	TGATCA	1 cut(s)	903
Kzo9I	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
LguI	GCTCTTC	1 cut(s)	611
LmnI	GCTCC	5 cut(s)	64, 85, 109, 343, 529
Lsp1109I	GCAGC	8 cut(s)	159, 213, 298, 614, 752, 877, 899, 1412
LweI	GCATC	5 cut(s)	346, 487, 565, 1103, 1173
MaeI	CTAG	3 cut(s)	200, 800, 1050
MaeII	ACGT	4 cut(s)	218, 251, 297, 1194
MaeIII	GTNAC	3 cut(s)	583, 964, 983
MalI	GATC	6 cut(s)	90, 329, 452, 611, 905, 1292
MbiI	CCGCTC	2 cut(s)	80, 424
MboI	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
MflI	RGATCY	2 cut(s)	327, 609
MhlI	GDGCHC	7 cut(s)	114, 260, 393, 534, 573, 762, 1194
MluCI	AATT	7 cut(s)	4, 155, 175, 507, 1017, 1074, 1086
MlyI	GAGTC	4 cut(s)	199, 379, 941, 1021
MmeI	TCCRAC	2 cut(s)	233, 1250
Mph1103I	ATGCAT	1 cut(s)	561
MroI	TCCGGA	1 cut(s)	340
MroNI	GCCGGC	2 cut(s)	548, 1298
MseI	TTAA	1 cut(s)	975
MslI	CAYNNNNRTG	1 cut(s)	564
MspA1I	CMGCKG	2 cut(s)	226, 890
MspR9I	CCNGG	4 cut(s)	59, 84, 436, 448
MvaI	CCWGG	2 cut(s)	84, 448
MvnI	CGCG	4 cut(s)	281, 896, 928, 930
NaeI	GCCGGC	2 cut(s)	550, 1300
NciI	CCSGG	2 cut(s)	59, 436
NcoI	CCATGG	2 cut(s)	847, 1209
NdeII	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
NgoMIV	GCCGGC	2 cut(s)	548, 1298
NheI	GCTAGC	1 cut(s)	799
NlaIV	GGNNCC	6 cut(s)	66, 329, 457, 1224, 1245, 1339
NmeAIII	GCCGAG	2 cut(s)	412, 1097
NmuCI	GTSAC	3 cut(s)	583, 964, 983
NruI	TCGCGA	1 cut(s)	281
NsiI	ATGCAT	1 cut(s)	561
NspI	RCATGY	2 cut(s)	563, 584
PaeI	GCATGC	1 cut(s)	563
PciI	ACATGT	1 cut(s)	580
PciSI	GCTCTTC	1 cut(s)	611
PcsI	WCGNNNNNNNCGW	2 cut(s)	360, 932
PdiI	GCCGGC	2 cut(s)	550, 1300
PfeI	GAWTC	2 cut(s)	263, 439
PflFI	GACNNNGTC	3 cut(s)	73, 217, 250
PinAI	ACCGGT	1 cut(s)	414
PleI	GAGTC	4 cut(s)	199, 378, 941, 1021
PmaCI	CACGTG	1 cut(s)	1195
PmlI	CACGTG	1 cut(s)	1195
PpsI	GAGTC	4 cut(s)	199, 378, 941, 1021
Ppu21I	YACGTR	1 cut(s)	1195
PscI	ACATGT	1 cut(s)	580
Psp124BI	GAGCTC	2 cut(s)	114, 393
Psp6I	CCWGG	2 cut(s)	82, 446
PspCI	CACGTG	1 cut(s)	1195
PspGI	CCWGG	2 cut(s)	82, 446
PspN4I	GGNNCC	6 cut(s)	66, 329, 457, 1224, 1245, 1339
PspPI	GGNCC	5 cut(s)	384, 464, 791, 840, 1223
PsuI	RGATCY	2 cut(s)	327, 609
PsyI	GACNNNGTC	3 cut(s)	73, 217, 250
PvuII	CAGCTG	1 cut(s)	890
RruI	TCGCGA	1 cut(s)	281
RseI	CAYNNNNRTG	1 cut(s)	564
SacI	GAGCTC	2 cut(s)	114, 393
SalI	GTCGAC	1 cut(s)	119
SapI	GCTCTTC	1 cut(s)	611
SaqAI	TTAA	1 cut(s)	975
Sau3AI	GATC	6 cut(s)	88, 327, 450, 609, 903, 1290
Sau96I	GGNCC	5 cut(s)	384, 464, 791, 840, 1223
SchI	GAGTC	4 cut(s)	199, 379, 941, 1021
ScrFI	CCNGG	4 cut(s)	59, 84, 436, 448
SduI	GDGCHC	7 cut(s)	114, 260, 393, 534, 573, 762, 1194
SfaNI	GCATC	5 cut(s)	346, 487, 565, 1103, 1173
SfcI	CTRYAG	2 cut(s)	519, 645
SinI	GGWCC	4 cut(s)	464, 791, 840, 1223
SmiMI	CAYNNNNRTG	1 cut(s)	564
SphI	GCATGC	1 cut(s)	563
Sse9I	AATT	7 cut(s)	4, 155, 175, 507, 1017, 1074, 1086
SspMI	CTAG	3 cut(s)	200, 800, 1050
SstI	GAGCTC	2 cut(s)	114, 393
StyD4I	CCNGG	4 cut(s)	57, 82, 434, 446
StyI	CCWWGG	3 cut(s)	847, 1209, 1332
TaaI	ACNGT	5 cut(s)	125, 646, 901, 1126, 1168
TaiI	ACGT	4 cut(s)	221, 254, 300, 1197
TasI	AATT	7 cut(s)	4, 155, 175, 507, 1017, 1074, 1086
TauI	GCSGC	3 cut(s)	164, 186, 896
TfiI	GAWTC	2 cut(s)	263, 439
Tru1I	TTAA	1 cut(s)	975
Tru9I	TTAA	1 cut(s)	975
TseFI	GTSAC	3 cut(s)	583, 964, 983
TseI	GCWGC	8 cut(s)	147, 226, 286, 602, 740, 887, 890, 1400
Tsp45I	GTSAC	3 cut(s)	583, 964, 983
TspDTI	ATGAA	2 cut(s)	17, 1360
TspGWI	ACGGA	1 cut(s)	952
Tth111I	GACNNNGTC	3 cut(s)	73, 217, 250
VpaK11BI	GGWCC	4 cut(s)	464, 791, 840, 1223
XagI	CCTNNNNNAGG	1 cut(s)	635
XapI	RAATTY	1 cut(s)	4
XceI	RCATGY	2 cut(s)	563, 584
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1216
XmiI	GTMKAC	1 cut(s)	120
XspI	CTAG	3 cut(s)	200, 800, 1050
ZraI	GACGTC	2 cut(s)	219, 252
Zsp2I	ATGCAT	1 cut(s)	561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.