MD08G1161700.v1.1

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0010157
Physical Location & Seq
Reverse (-)
18903915 .. 18910461
6547 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1161700.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGATTTTTAATCAAATAAAAATAAAATTATTTTCCATAAATTCAGTGTTCATAATCTCCGTCTTCCTCCTCGGTTCGTTAATTTCACCAGCGGAACCGACGTCGTCCGGCGATCACAGCCACCCGTTCGTCGAGCTCGATAACTGTTGCCCCAGATGCAAGCCAAGCCCCGAAGCTCGAAAGAATCTGAGCCTCCACGGGCTAACATCTCCACCGTCACCCTCGACGTCGTCTCCGCCTCCTCGGCCGAGCTGCGAGGTTTGGACTGAGGCGTGCTCGGAAGCTGTTTTGAGCTTCGCGAAGCTACCGGAGAACATGGAGTGGCTGAAGACGGTGAGGCGGAGGATCCACGAGCATCCGGAGCTCGCTTTCGAGGAGTTCGAGACGAGCCGGTTGGTTCGGGATGAGTTGGACCGGTTGGACATCGGTTACCGGTTTCCTTTGGCAAAGACCGGAATCCGAGCTTGGATCGGAACCGGCGGTCCTCCGTTTGTCGCCGTCAGAGCTGACATGGACGCTCTTCCAATTCAGGAGGCTGTGGAATGGGAGCACAAGAGCAAAGTAGCCGGCAAAATGCATGCTTGTGGGCACGATGCGCATGTGGCTATGCTAATTGGCGCTGCCAAGATCTTGAAGAGCCGTGAGCACCTTCTCAAGGGAACTGTAATATTGCTATTTCAGCCGGCGGAGGAAGCCGGTAATGGTGCAAAGAGAATGATCGGAGATGGCGCTTTGGAGGATGTGGAGGCCATATTTGCAGCTCATGTATCCCATGAGCACCCAACTGGCGTCATCGGATCAAGGGCTGGACCTCTCCTAGCCGGCTGTGGCTTCTTCCGAGCTGTTATCAGTGGAACCACAGGCCAAGCTGAAAGCCCCCCCATCTCTACTGACCCTGTTTTGGCTGCTGCCGCAGCTGTCATCAGCTTGCAGGGCATCGTGTCTCGCGAATCAAACCCTTTGGACTCTCAGGTTGTTTCTGTGACTGCATTCAATGGAGGCGATGATCTTGGGATGATACCAAACACTGTGGTACTTGGCGGTACTTTCAGGGCTTTCTCCAACACAAGTTTCTACAGAATTCTTCAAAGAATTGAAGAGGTAATTGTGGAGCAAGCAAGCGTTTACAGATGCTCAGCAACAGTAGACTTCTTCAAAAACCAGAACACCATATACCCACCAACAGTGAACGATGAGAAAATGCATGAGCATGTGACCAAAGTGGCCGTCGACTTGCTGGGCCCCACTAACTTCAGGGTGGTGCCACCCATGATGGGGGCGGAGGACTTCTCATTCTACTCGGAGGTCATCCCTGCCGGCTTCTTCTACATCGGGATCAGGAACGAGACCTTGGGTTCCACACACACAGGTCACTCTCCTTATTTTTTCATTGACGAAGATGTTTTGCCCATTGGGGCTGCAACTCATGCCACAATTGCAGAAAGGTACCTCAACGAGCGGTCGCGGGTGATCACAGACGGGTGA

Protein Analysis

495

Amino Acids

53.75

Weight (kDa)

5.71

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 166 - 481 3.4e-44 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 104, 230
Acc16I TGCGCA 1 cut(s) 597
Acc65I GGTACC 1 cut(s) 1446
AccB1I GGYRCC 2 cut(s) 1261, 1446
AccBSI CCGCTC 1 cut(s) 1459
AccI GTMKAC 2 cut(s) 1146, 1230
AccII CGCG 3 cut(s) 299, 948, 1465
AccIII TCCGGA 1 cut(s) 358
AclWI GGATC 5 cut(s) 340, 353, 476, 805, 1343
AcoI YGGCCR 2 cut(s) 245, 1224
AcsI RAATTY 2 cut(s) 40, 1080
AcuI CTGAAG 2 cut(s) 347, 1237
AcyI GRCGYC 3 cut(s) 101, 227, 789
AfaI GTAC 3 cut(s) 1035, 1045, 1448
AfiI CCNNNNNNNGG 4 cut(s) 655, 901, 1274, 1275
AgeI ACCGGT 2 cut(s) 414, 432
AgsI TTSAA 5 cut(s) 634, 994, 1088, 1097, 1156
AjuI GAANNNNNNNTTGG 6 cut(s) 448, 480, 1055, 1087, 1334, 1366
AloI GAACNNNNNNTCC 2 cut(s) 466, 498
Alw21I GWGCWC 6 cut(s) 138, 278, 366, 552, 648, 780
Alw26I GTCTC 4 cut(s) 237, 377, 948, 1340
AlwI GGATC 5 cut(s) 340, 353, 476, 805, 1343
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 5 cut(s) 245, 747, 862, 1224, 1240
ApaI GGGCCC 1 cut(s) 1244
ApeKI GCWGC 7 cut(s) 252, 620, 758, 905, 908, 914, 1418
ApoI RAATTY 2 cut(s) 40, 1080
ArsI GACNNNNNNTTYG 2 cut(s) 1212, 1244
AsiGI ACCGGT 2 cut(s) 414, 432
Asp718I GGTACC 1 cut(s) 1446
AspLEI GCGC 3 cut(s) 598, 620, 731
AspS9I GGNCC 5 cut(s) 412, 482, 809, 1240, 1241
AsuHPI GGTGA 4 cut(s) 78, 210, 346, 1480
AvaII GGWCC 3 cut(s) 412, 482, 809
BaeGI GKGCMC 2 cut(s) 591, 1244
BamHI GGATCC 1 cut(s) 345
BanI GGYRCC 2 cut(s) 1261, 1446
BanII GRGCYC 3 cut(s) 138, 366, 1244
BauI CACGAG 1 cut(s) 350
BbsI GAAGAC 2 cut(s) 55, 335
Bbv12I GWGCWC 6 cut(s) 138, 278, 366, 552, 648, 780
BbvI GCAGC 7 cut(s) 239, 607, 770, 892, 895, 926, 1405
BccI CCATC 3 cut(s) 719, 890, 1267
BceAI ACGGC 3 cut(s) 482, 624, 1211
BciVI GTATCC 1 cut(s) 778
BclI TGATCA 1 cut(s) 1470
BcoDI GTCTC 4 cut(s) 237, 377, 948, 1340
BfaI CTAG 1 cut(s) 818
BfmI CTRYAG 1 cut(s) 1075
BfoI RGCGCY 2 cut(s) 621, 732
BfuI GTATCC 1 cut(s) 778
BglI GCCNNNNNGGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 627
BisI GCNGC 8 cut(s) 253, 621, 759, 906, 909, 912, 915, 1419
BlpI GCTNAGC 1 cut(s) 1135
BlsI GCNGC 8 cut(s) 254, 622, 760, 907, 910, 913, 916, 1420
Bme18I GGWCC 3 cut(s) 412, 482, 809
BmgT120I GGNCC 5 cut(s) 412, 482, 809, 1240, 1241
BmiI GGNNCC 9 cut(s) 96, 347, 475, 856, 1242, 1243, 1263, 1357, 1448
BmsI GCATC 5 cut(s) 146, 364, 583, 945, 1121
BpiI GAAGAC 2 cut(s) 55, 335
BplI GAGNNNNNCTC 4 cut(s) 260, 292, 1274, 1306
Bpu1102I GCTNAGC 1 cut(s) 1135
BpuEI CTTGAG 1 cut(s) 638
BsaHI GRCGYC 3 cut(s) 101, 227, 789
BsaI GGTCTC 1 cut(s) 1340
BsaJI CCNNGG 4 cut(s) 70, 196, 242, 1350
BsaWI WCCGGW 5 cut(s) 307, 358, 414, 432, 452
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 4 cut(s) 655, 901, 1274, 1275
Bse118I RCCGGY 9 cut(s) 390, 414, 432, 476, 566, 682, 695, 821, 1316
Bse1I ACTGG 1 cut(s) 790
BseAI TCCGGA 1 cut(s) 358
BseDI CCNNGG 4 cut(s) 70, 196, 242, 1350
BseGI GGATG 5 cut(s) 355, 409, 745, 1020, 1308
BseLI CCNNNNNNNGG 4 cut(s) 655, 901, 1274, 1275
BseMII CTCAG 4 cut(s) 179, 258, 983, 1149
BseNI ACTGG 1 cut(s) 790
BseRI GAGGAG 3 cut(s) 59, 231, 389
BseSI GKGCMC 2 cut(s) 591, 1244
BseX3I CGGCCG 1 cut(s) 245
BseXI GCAGC 7 cut(s) 239, 607, 770, 892, 895, 926, 1405
BseYI CCCAGC 1 cut(s) 1237
Bsh1236I CGCG 3 cut(s) 299, 948, 1465
Bsh1285I CGRYCG 2 cut(s) 248, 1463
BshFI GGCC 5 cut(s) 247, 749, 864, 1226, 1242
BshNI GGYRCC 2 cut(s) 1261, 1446
BshTI ACCGGT 2 cut(s) 414, 432
BsiEI CGRYCG 2 cut(s) 248, 1463
BsiHKAI GWGCWC 6 cut(s) 138, 278, 366, 552, 648, 780
BslI CCNNNNNNNGG 4 cut(s) 655, 901, 1274, 1275
BsmAI GTCTC 4 cut(s) 237, 377, 948, 1340
BsmBI CGTCTC 2 cut(s) 237, 377
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 5 cut(s) 247, 749, 864, 1226, 1242
Bso31I GGTCTC 1 cut(s) 1340
Bsp120I GGGCCC 1 cut(s) 1240
Bsp1286I GDGCHC 8 cut(s) 138, 278, 366, 552, 591, 648, 780, 1244
Bsp13I TCCGGA 1 cut(s) 358
Bsp143I GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
Bsp1720I GCTNAGC 1 cut(s) 1135
Bsp68I TCGCGA 2 cut(s) 299, 948
BspANI GGCC 5 cut(s) 247, 749, 864, 1226, 1242
BspCNI CTCAG 4 cut(s) 180, 259, 982, 1148
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 3 cut(s) 299, 948, 1465
BspLI GGNNCC 9 cut(s) 96, 347, 475, 856, 1242, 1243, 1263, 1357, 1448
BspPI GGATC 5 cut(s) 340, 353, 476, 805, 1343
BspQI GCTCTTC 2 cut(s) 525, 629
BspT107I GGYRCC 2 cut(s) 1261, 1446
BspTNI GGTCTC 1 cut(s) 1340
BsrBI CCGCTC 1 cut(s) 1459
BsrFI RCCGGY 9 cut(s) 390, 414, 432, 476, 566, 682, 695, 821, 1316
BsrI ACTGG 1 cut(s) 790
BssAI RCCGGY 9 cut(s) 390, 414, 432, 476, 566, 682, 695, 821, 1316
BssECI CCNNGG 4 cut(s) 70, 196, 242, 1350
BssMI GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
BssNI GRCGYC 3 cut(s) 101, 227, 789
BssSI CACGAG 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 1350
Bst2BI CACGAG 1 cut(s) 350
Bst4CI ACNGT 7 cut(s) 146, 216, 334, 664, 1030, 1144, 1186
Bst6I CTCTTC 3 cut(s) 525, 629, 1092
BstACI GRCGYC 3 cut(s) 101, 227, 789
BstAPI GCANNNNNTGC 1 cut(s) 1427
BstDEI CTNAG 4 cut(s) 188, 267, 969, 1135
BstDSI CCRYGG 1 cut(s) 196
BstEII GGTNACC 1 cut(s) 428
BstENI CCTNNNNNAGG 1 cut(s) 653
BstF5I GGATG 5 cut(s) 355, 409, 745, 1020, 1308
BstFNI CGCG 3 cut(s) 299, 948, 1465
BstH2I RGCGCY 2 cut(s) 621, 732
BstHHI GCGC 3 cut(s) 598, 620, 731
BstKTI GATC 9 cut(s) 115, 348, 471, 630, 720, 800, 1009, 1338, 1473
BstMAI GTCTC 4 cut(s) 237, 377, 948, 1340
BstMBI GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
BstMCI CGRYCG 2 cut(s) 248, 1463
BstNSI RCATGY 3 cut(s) 581, 602, 1214
BstPI GGTNACC 1 cut(s) 428
BstSFI CTRYAG 1 cut(s) 1075
BstSLI GKGCMC 2 cut(s) 591, 1244
BstUI CGCG 3 cut(s) 299, 948, 1465
BstV1I GCAGC 7 cut(s) 239, 607, 770, 892, 895, 926, 1405
BstV2I GAAGAC 2 cut(s) 55, 335
BstX2I RGATCY 2 cut(s) 345, 627
BstYI RGATCY 2 cut(s) 345, 627
BstZI CGGCCG 1 cut(s) 245
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 5 cut(s) 247, 749, 864, 1226, 1242
BtgI CCRYGG 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 1017
BtsCI GGATG 5 cut(s) 355, 409, 745, 1020, 1308
BtsIMutI CAGTG 4 cut(s) 51, 856, 1026, 1191
BtuMI TCGCGA 2 cut(s) 299, 948
CfoI GCGC 3 cut(s) 598, 620, 731
Cfr10I RCCGGY 9 cut(s) 390, 414, 432, 476, 566, 682, 695, 821, 1316
Cfr13I GGNCC 5 cut(s) 412, 482, 809, 1240, 1241
CseI GACGC 2 cut(s) 524, 778
Csp6I GTAC 3 cut(s) 1034, 1044, 1447
CspAI ACCGGT 2 cut(s) 414, 432
CspCI CAANNNNNGTGG 2 cut(s) 1011, 1046
CviQI GTAC 3 cut(s) 1034, 1044, 1447
DdeI CTNAG 4 cut(s) 188, 267, 969, 1135
DpnI GATC 9 cut(s) 114, 347, 470, 629, 719, 799, 1008, 1337, 1472
DpnII GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
EaeI YGGCCR 2 cut(s) 245, 1224
EagI CGGCCG 1 cut(s) 245
Eam1104I CTCTTC 3 cut(s) 525, 629, 1092
EarI CTCTTC 3 cut(s) 525, 629, 1092
EciI GGCGGA 4 cut(s) 225, 355, 701, 1295
Ecl136II GAGCTC 2 cut(s) 136, 364
EclXI CGGCCG 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 1350
Eco24I GRGCYC 3 cut(s) 138, 366, 1244
Eco31I GGTCTC 1 cut(s) 1340
Eco47I GGWCC 3 cut(s) 412, 482, 809
Eco52I CGGCCG 1 cut(s) 245
Eco53kI GAGCTC 2 cut(s) 136, 364
Eco57I CTGAAG 2 cut(s) 347, 1237
Eco91I GGTNACC 1 cut(s) 428
EcoICRI GAGCTC 2 cut(s) 136, 364
EcoNI CCTNNNNNAGG 1 cut(s) 653
EcoO109I RGGNCCY 1 cut(s) 1241
EcoO65I GGTNACC 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 1080
EcoT14I CCWWGG 1 cut(s) 1350
EcoT22I ATGCAT 2 cut(s) 579, 1206
EcoT38I GRGCYC 3 cut(s) 138, 366, 1244
ErhI CCWWGG 1 cut(s) 1350
Esp3I CGTCTC 2 cut(s) 237, 377
FauI CCCGC 1 cut(s) 1458
FbaI TGATCA 1 cut(s) 1470
FblI GTMKAC 2 cut(s) 1146, 1230
Fnu4HI GCNGC 8 cut(s) 253, 621, 759, 906, 909, 912, 915, 1419
FokI GGATG 5 cut(s) 342, 416, 752, 1027, 1295
FriOI GRGCYC 3 cut(s) 138, 366, 1244
Fsp4HI GCNGC 8 cut(s) 253, 621, 759, 906, 909, 912, 915, 1419
FspAI RTGCGCAY 1 cut(s) 597
FspBI CTAG 1 cut(s) 818
FspI TGCGCA 1 cut(s) 597
GlaI GCGC 3 cut(s) 597, 619, 730
GluI GCNGC 8 cut(s) 253, 621, 759, 906, 909, 912, 915, 1419
GsaI CCCAGC 1 cut(s) 1241
HaeII RGCGCY 2 cut(s) 621, 732
HaeIII GGCC 5 cut(s) 247, 749, 864, 1226, 1242
HgaI GACGC 2 cut(s) 524, 778
HhaI GCGC 3 cut(s) 598, 620, 731
Hin1I GRCGYC 3 cut(s) 101, 227, 789
Hin6I GCGC 3 cut(s) 596, 618, 729
HinP1I GCGC 3 cut(s) 596, 618, 729
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HinfI GANTC 4 cut(s) 184, 456, 950, 965
HphI GGTGA 4 cut(s) 78, 210, 346, 1480
Hpy166II GTNNAC 4 cut(s) 1126, 1147, 1189, 1231
Hpy188I TCNGA 9 cut(s) 189, 280, 461, 473, 503, 722, 797, 839, 1303
Hpy188III TCNNGA 9 cut(s) 298, 359, 382, 401, 530, 631, 947, 1333, 1339
Hpy8I GTNNAC 4 cut(s) 1126, 1147, 1189, 1231
Hpy99I CGWCG 6 cut(s) 103, 106, 134, 229, 232, 1232
HpyAV CCTTC 1 cut(s) 659
HpyCH4III ACNGT 7 cut(s) 146, 216, 334, 664, 1030, 1144, 1186
HpyCH4IV ACGT 2 cut(s) 101, 227
HpyCH4V TGCA 9 cut(s) 159, 577, 707, 758, 931, 989, 1204, 1421, 1439
HpyF3I CTNAG 4 cut(s) 188, 267, 969, 1135
HpySE526I ACGT 2 cut(s) 101, 227
Hsp92I GRCGYC 3 cut(s) 101, 227, 789
HspAI GCGC 3 cut(s) 596, 618, 729
Kpn2I TCCGGA 1 cut(s) 358
KpnI GGTACC 1 cut(s) 1450
KroI GCCGGC 4 cut(s) 566, 682, 821, 1316
KroNI GCCGGC 4 cut(s) 568, 684, 823, 1318
Ksp22I TGATCA 1 cut(s) 1470
Kzo9I GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
LguI GCTCTTC 2 cut(s) 525, 629
LmnI GCTCC 3 cut(s) 361, 547, 1111
Lsp1109I GCAGC 7 cut(s) 239, 607, 770, 892, 895, 926, 1405
LweI GCATC 5 cut(s) 146, 364, 583, 945, 1121
MaeI CTAG 1 cut(s) 818
MaeII ACGT 2 cut(s) 101, 227
MaeIII GTNAC 5 cut(s) 216, 428, 982, 1213, 1370
MalI GATC 9 cut(s) 114, 347, 470, 629, 719, 799, 1008, 1337, 1472
MbiI CCGCTC 1 cut(s) 1459
MboI GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
MfeI CAATTG 1 cut(s) 1434
MflI RGATCY 2 cut(s) 345, 627
MhlI GDGCHC 8 cut(s) 138, 278, 366, 552, 591, 648, 780, 1244
MluCI AATT 9 cut(s) 26, 40, 81, 525, 612, 1080, 1092, 1104, 1434
MlyI GAGTC 1 cut(s) 959
MmeI TCCRAC 3 cut(s) 390, 399, 1086
Mph1103I ATGCAT 2 cut(s) 579, 1206
MroI TCCGGA 1 cut(s) 358
MroNI GCCGGC 4 cut(s) 566, 682, 821, 1316
MseI TTAA 2 cut(s) 9, 80
MslI CAYNNNNRTG 2 cut(s) 582, 1209
MspA1I CMGCKG 2 cut(s) 92, 917
MunI CAATTG 1 cut(s) 1434
Mva1269I GAATGC 1 cut(s) 989
MvnI CGCG 3 cut(s) 299, 948, 1465
NaeI GCCGGC 4 cut(s) 568, 684, 823, 1318
NdeII GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
NgoMIV GCCGGC 4 cut(s) 566, 682, 821, 1316
NlaIV GGNNCC 9 cut(s) 96, 347, 475, 856, 1242, 1243, 1263, 1357, 1448
NmeAIII GCCGAG 2 cut(s) 223, 273
NmuCI GTSAC 4 cut(s) 216, 982, 1213, 1370
NruI TCGCGA 2 cut(s) 299, 948
NsbI TGCGCA 1 cut(s) 597
NsiI ATGCAT 2 cut(s) 579, 1206
NspI RCATGY 3 cut(s) 581, 602, 1214
PaeI GCATGC 1 cut(s) 581
PciSI GCTCTTC 2 cut(s) 525, 629
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 378, 945
PctI GAATGC 1 cut(s) 989
PdiI GCCGGC 4 cut(s) 568, 684, 823, 1318
PfeI GAWTC 3 cut(s) 184, 456, 950
PflFI GACNNNGTC 2 cut(s) 103, 229
PinAI ACCGGT 2 cut(s) 414, 432
PkrI GCNGC 8 cut(s) 254, 622, 760, 907, 910, 913, 916, 1420
PleI GAGTC 1 cut(s) 959
PpsI GAGTC 1 cut(s) 959
Psp124BI GAGCTC 2 cut(s) 138, 366
PspEI GGTNACC 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 1237
PspN4I GGNNCC 9 cut(s) 96, 347, 475, 856, 1242, 1243, 1263, 1357, 1448
PspOMI GGGCCC 1 cut(s) 1240
PspPI GGNCC 5 cut(s) 412, 482, 809, 1240, 1241
PsrI GAACNNNNNNTAC 2 cut(s) 1157, 1189
PsuI RGATCY 2 cut(s) 345, 627
PsyI GACNNNGTC 2 cut(s) 103, 229
PvuII CAGCTG 1 cut(s) 917
RruI TCGCGA 2 cut(s) 299, 948
RsaI GTAC 3 cut(s) 1035, 1045, 1448
RsaNI GTAC 3 cut(s) 1034, 1044, 1447
RseI CAYNNNNRTG 2 cut(s) 582, 1209
SacI GAGCTC 2 cut(s) 138, 366
SalI GTCGAC 1 cut(s) 1229
SapI GCTCTTC 2 cut(s) 525, 629
SaqAI TTAA 2 cut(s) 9, 80
SatI GCNGC 8 cut(s) 253, 621, 759, 906, 909, 912, 915, 1419
Sau3AI GATC 9 cut(s) 112, 345, 468, 627, 717, 797, 1006, 1335, 1470
Sau96I GGNCC 5 cut(s) 412, 482, 809, 1240, 1241
SchI GAGTC 1 cut(s) 959
SduI GDGCHC 8 cut(s) 138, 278, 366, 552, 591, 648, 780, 1244
SfaNI GCATC 5 cut(s) 146, 364, 583, 945, 1121
SfcI CTRYAG 1 cut(s) 1075
SinI GGWCC 3 cut(s) 412, 482, 809
SmiMI CAYNNNNRTG 2 cut(s) 582, 1209
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
SphI GCATGC 1 cut(s) 581
Sse9I AATT 9 cut(s) 26, 40, 81, 525, 612, 1080, 1092, 1104, 1434
SspI AATATT 1 cut(s) 669
SspMI CTAG 1 cut(s) 818
SstI GAGCTC 2 cut(s) 138, 366
StyI CCWWGG 1 cut(s) 1350
TaaI ACNGT 7 cut(s) 146, 216, 334, 664, 1030, 1144, 1186
TaiI ACGT 2 cut(s) 104, 230
TaqI TCGA 7 cut(s) 132, 138, 178, 224, 372, 381, 1230
TasI AATT 9 cut(s) 26, 40, 81, 525, 612, 1080, 1092, 1104, 1434
TauI GCSGC 1 cut(s) 914
TfiI GAWTC 3 cut(s) 184, 456, 950
Tru1I TTAA 2 cut(s) 9, 80
Tru9I TTAA 2 cut(s) 9, 80
TscAI CASTG 4 cut(s) 51, 856, 1033, 1191
TseFI GTSAC 4 cut(s) 216, 982, 1213, 1370
TseI GCWGC 7 cut(s) 252, 620, 758, 905, 908, 914, 1418
Tsp45I GTSAC 4 cut(s) 216, 982, 1213, 1370
TspDTI ATGAA 2 cut(s) 40, 1378
TspGWI ACGGA 2 cut(s) 49, 477
TspRI CASTG 4 cut(s) 51, 856, 1033, 1191
Tth111I GACNNNGTC 2 cut(s) 103, 229
VpaK11BI GGWCC 3 cut(s) 412, 482, 809
XagI CCTNNNNNAGG 1 cut(s) 653
XapI RAATTY 2 cut(s) 40, 1080
XceI RCATGY 3 cut(s) 581, 602, 1214
XmiI GTMKAC 2 cut(s) 1146, 1230
XspI CTAG 1 cut(s) 818
ZraI GACGTC 2 cut(s) 102, 228
Zsp2I ATGCAT 2 cut(s) 579, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.