Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010157

Physical Location & Seq

Genomic Coordinates Reverse (-)

40886134 .. 40891788

Gene Structure 5655 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1346800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGAATTTTAATCAAACCAAAACAAAATTAATCTACACAAATTCAATATTAATAATCTCCATCTTCGTCCTCGGTTCGATAATTTCACCCGCCGAGACGACTCCGTCCGGCGATCACAGCCACCCGTTCTTCGAGCTCGATGACTGTTGCCCCAGCTGCAAGGCAAGCCCCGAAGCTCGAAAGAATCTGAGCCTCCAAGCGCTAACCTCTCCGCCAACATCACCGCCGTCGTCGCCTCCTCAGAGCTGCGAGGTTTGGACGGAGGCGTGCTCGGTAGCGGTTTTGAGCTTGGCGAAGCGACCGGAGAACGTGGAGTGGTTGAAGACGGTGAGGCGGAGGATCCACGAGCATCCAGAGCTCGCTTTCGAGGAGTTCGAGACGAGCCGGCTGGTTCGGGATGAGTTGGACCGGTTGGATATCGGTTACCGGTATCCTTTGGCTAAAACCGGAATCCGAGCTTGGATCGGAACCGGCGGTCCGCCGTTTGTCGCCGTCAGGGCTGACATGGACGCCCTTCCAATTCAGGAAGCTGTTGAATGGGAGCACAAGAGCAAAGTAGCCGGCAAAATGCACGCTTGTGGGCACGATGCGCATGTGACTATGCTAATTGGCGCCGCCAAGATCCTGAAGAACCGTGAACACCTTCTGAAGGGAACTGTAATATTGCTGTTTCAGCCAGCGGAGGAAGCCGGTAATGGTGCAAAGCAAATGATCGGAGATGATGCTTTGGAGGATGTGGAGGCCATATTTGCAGCTCATGTATCCCATGAGCACCCTACTGGCGTCATCGGGTCAAGGCCTGGTCCCTTGCTAGCTGGCTGTGGCTTCTTCAGAGCTGTTATCAGTGGAACCACAGGATCTACTGACCCTGTTTTGGCAGCCGCCACGGCCGTCATCAGCTTGCAGGGCATCGTATCCCGTGAATCAAACCCTTTGGACTCGCAGGTTGTTTCTGTAACTGCATTCAATGGAGGCGATGATCTTGGGATGATACCCAACACTGTGGTACTTGGTGGTACTTTCAGGGCTTTCTCCAACACAAACTTCTACAGAATTCTTCAAAGAATTGAAGAGGTAATTGTGGAGCAAGCAAGTGTTTACAGATGCTCAGCAACAGTGGACTTCTTCGAAAACCAGAACACCATCTACCCTCCAACAGTGAACGATGAGAAAATGCATGAGCATGTGAGCAAAGTGGCCATCGATTTGCTGGGCCCCACCAACTTCAGGGTGGTGCCACCCATGATGGGGGCGGAGGACTTCTCGTTCTACTCGGAGGTCATCCCTGCTGGCTTCTTCTACATCGGGATCAGGAACGAGACCTTGGGGTCCACACACACGGGCCACTCTCCTTATTTTTTCATCGACGAAGATGTTTTGCCAATTGGGGCTGCAACTCATGCCACAATTGCAGAAAGGTACCTCAACGAGCGGTCGCGGGTGACCACAGACGGGTTATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0006082 GO:0008150 GO:0008152 GO:0009694 GO:0009719 GO:0009725 GO:0009753 GO:0009987 GO:0010033 GO:0010112 GO:0010817 GO:0016787 GO:0016810 GO:0019752 GO:0031347 GO:0032101 GO:0032787 GO:0042221 GO:0042445 GO:0043436 GO:0043900 GO:0044237 GO:0044281 GO:0045088 GO:0048583 GO:0050776 GO:0050789 GO:0050896 GO:0065007 GO:0065008 GO:0071704 GO:0080134 GO:1901700 GO:1990206

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

488

Amino Acids

53.16

Weight (kDa)

5.4

Isoelectric Point (pI)

40.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	164 - 472	4.4e-44	Peptidase family M20/M25/M40

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010157)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G44350
fragaria_vesca	FvH4_2g27160 FvH4_2g27160
malus_domestica	MD08G1161700.v1.1 MD15G1346800.v1.1
prunus_persica	Prupe.1G494400_v2.0.a1 Prupe.1G494400_v2.0.a1
pyrus_communis	pycom08g13900 pycom15g31060
rosa_chinensis	RchiOBHm_Chr6g0296071
rosa_laevigata	RLG00000011736
rosa_multiflora	Rmu_sc0003139.1_g000031 Rmu_sc0004275.1_g000018
rosa_roxburghii	Rroxscaffold_7G00171850
rosa_rugosa	Rorug06G0256900 Rorug06G0257000
rosa_samantha	Rh6AG370300 Rh6BG377400 Rh6CG383300 Rh6DG370100
rosa_wichuraiana	Rw6G032270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	103, 1327
Acc16I	TGCGCA	1 cut(s)	591
Acc36I	ACCTGC	1 cut(s)	934
Acc65I	GGTACC	1 cut(s)	1419
AccB1I	GGYRCC	3 cut(s)	611, 1234, 1419
AccBSI	CCGCTC	1 cut(s)	1432
AccII	CGCG	1 cut(s)	1438
AclWI	GGATC	6 cut(s)	334, 347, 470, 616, 865, 1316
AcoI	YGGCCR	2 cut(s)	888, 1197
AcsI	RAATTY	3 cut(s)	4, 40, 1053
AcuI	CTGAAG	4 cut(s)	647, 668, 814, 1210
AcyI	GRCGYC	3 cut(s)	510, 612, 783
AfaI	GTAC	3 cut(s)	1008, 1018, 1421
AfeI	AGCGCT	1 cut(s)	201
AfiI	CCNNNNNNNGG	6 cut(s)	649, 874, 1227, 1247, 1248, 1452
AgeI	ACCGGT	2 cut(s)	408, 426
AgsI	TTSAA	6 cut(s)	45, 322, 536, 967, 1061, 1070
AjnI	CCWGG	1 cut(s)	799
AjuI	GAANNNNNNNTTGG	6 cut(s)	442, 474, 1028, 1060, 1307, 1339
AleI	CACNNNNGTG	1 cut(s)	576
AloI	GAACNNNNNNTCC	4 cut(s)	460, 492, 1250, 1282
Alw21I	GWGCWC	5 cut(s)	138, 272, 360, 546, 774
Alw26I	GTCTC	3 cut(s)	89, 371, 1313
AlwI	GGATC	6 cut(s)	334, 347, 470, 616, 865, 1316
Aor51HI	AGCGCT	1 cut(s)	201
AoxI	GGCC	6 cut(s)	741, 797, 888, 1197, 1213, 1342
ApaI	GGGCCC	1 cut(s)	1217
ApeKI	GCWGC	5 cut(s)	156, 246, 752, 878, 1391
ApoI	RAATTY	3 cut(s)	4, 40, 1053
AseI	ATTAAT	2 cut(s)	29, 50
AsiGI	ACCGGT	2 cut(s)	408, 426
Asp700I	GAANNNNTTC	1 cut(s)	642
Asp718I	GGTACC	1 cut(s)	1419
AspLEI	GCGC	3 cut(s)	202, 592, 614
AspS9I	GGNCC	7 cut(s)	406, 476, 803, 1213, 1214, 1329, 1342
AsuHPI	GGTGA	4 cut(s)	78, 213, 340, 1453
AsuII	TTCGAA	1 cut(s)	1128
AsuNHI	GCTAGC	1 cut(s)	811
AvaII	GGWCC	4 cut(s)	406, 476, 803, 1329
BaeGI	GKGCMC	2 cut(s)	585, 1217
BalI	TGGCCA	1 cut(s)	1199
BamHI	GGATCC	1 cut(s)	339
BanI	GGYRCC	3 cut(s)	611, 1234, 1419
BanII	GRGCYC	3 cut(s)	138, 360, 1217
BauI	CACGAG	1 cut(s)	344
BbsI	GAAGAC	1 cut(s)	329
Bbv12I	GWGCWC	5 cut(s)	138, 272, 360, 546, 774
BbvI	GCAGC	5 cut(s)	143, 233, 764, 890, 1378
BccI	CCATC	4 cut(s)	68, 1151, 1208, 1240
BceAI	ACGGC	5 cut(s)	211, 466, 476, 875, 903
BciT130I	CCWGG	1 cut(s)	801
BciVI	GTATCC	3 cut(s)	441, 772, 925
BcoDI	GTCTC	3 cut(s)	89, 371, 1313
BfaI	CTAG	1 cut(s)	812
BfmI	CTRYAG	1 cut(s)	1048
BfoI	RGCGCY	2 cut(s)	203, 615
BfuAI	ACCTGC	1 cut(s)	934
BfuI	GTATCC	3 cut(s)	441, 772, 925
BglI	GCCNNNNNGGC	2 cut(s)	497, 887
BisI	GCNGC	7 cut(s)	157, 247, 615, 753, 879, 882, 1392
BlpI	GCTNAGC	1 cut(s)	1108
BlsI	GCNGC	7 cut(s)	158, 248, 616, 754, 880, 883, 1393
Bme1390I	CCNGG	1 cut(s)	801
Bme18I	GGWCC	4 cut(s)	406, 476, 803, 1329
BmgT120I	GGNCC	7 cut(s)	406, 476, 803, 1213, 1214, 1329, 1342
BmrFI	CCNGG	1 cut(s)	801
BmsI	GCATC	5 cut(s)	358, 577, 712, 918, 1094
BmtI	GCTAGC	1 cut(s)	815
BpiI	GAAGAC	1 cut(s)	329
BplI	GAGNNNNNCTC	4 cut(s)	254, 286, 1247, 1279
Bpu1102I	GCTNAGC	1 cut(s)	1108
Bpu14I	TTCGAA	1 cut(s)	1128
Bsa29I	ATCGAT	1 cut(s)	1203
BsaHI	GRCGYC	3 cut(s)	510, 612, 783
BsaI	GGTCTC	1 cut(s)	1313
BsaJI	CCNNGG	3 cut(s)	70, 885, 1323
BsaWI	WCCGGW	4 cut(s)	301, 408, 426, 446
Bsc4I	CCNNNNNNNGG	6 cut(s)	649, 874, 1227, 1247, 1248, 1452
Bse118I	RCCGGY	6 cut(s)	384, 408, 426, 470, 560, 689
Bse1I	ACTGG	1 cut(s)	784
BseBI	CCWGG	1 cut(s)	801
BseCI	ATCGAT	1 cut(s)	1203
BseDI	CCNNGG	3 cut(s)	70, 885, 1323
BseGI	GGATG	5 cut(s)	349, 403, 739, 993, 1281
BseLI	CCNNNNNNNGG	6 cut(s)	649, 874, 1227, 1247, 1248, 1452
BseMII	CTCAG	3 cut(s)	179, 254, 1122
BseNI	ACTGG	1 cut(s)	784
BseRI	GAGGAG	2 cut(s)	228, 383
BseSI	GKGCMC	2 cut(s)	585, 1217
BseX3I	CGGCCG	1 cut(s)	888
BseXI	GCAGC	5 cut(s)	143, 233, 764, 890, 1378
BseYI	CCCAGC	2 cut(s)	152, 1210
Bsh1236I	CGCG	1 cut(s)	1438
Bsh1285I	CGRYCG	3 cut(s)	302, 891, 1436
BshFI	GGCC	6 cut(s)	743, 799, 890, 1199, 1215, 1344
BshNI	GGYRCC	3 cut(s)	611, 1234, 1419
BshTI	ACCGGT	2 cut(s)	408, 426
BshVI	ATCGAT	1 cut(s)	1203
BsiEI	CGRYCG	3 cut(s)	302, 891, 1436
BsiHKAI	GWGCWC	5 cut(s)	138, 272, 360, 546, 774
BsiSI	CCGG	9 cut(s)	108, 302, 385, 409, 427, 447, 471, 561, 690
BslFI	GGGAC	1 cut(s)	789
BslI	CCNNNNNNNGG	6 cut(s)	649, 874, 1227, 1247, 1248, 1452
BsmAI	GTCTC	3 cut(s)	89, 371, 1313
BsmBI	CGTCTC	2 cut(s)	89, 371
BsmFI	GGGAC	1 cut(s)	789
BsmI	GAATGC	1 cut(s)	962
BsnI	GGCC	6 cut(s)	743, 799, 890, 1199, 1215, 1344
Bso31I	GGTCTC	1 cut(s)	1313
Bsp119I	TTCGAA	1 cut(s)	1128
Bsp120I	GGGCCC	1 cut(s)	1213
Bsp1286I	GDGCHC	7 cut(s)	138, 272, 360, 546, 585, 774, 1217
Bsp143I	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
Bsp1720I	GCTNAGC	1 cut(s)	1108
BspANI	GGCC	6 cut(s)	743, 799, 890, 1199, 1215, 1344
BspCNI	CTCAG	3 cut(s)	180, 253, 1121
BspDI	ATCGAT	1 cut(s)	1203
BspFNI	CGCG	1 cut(s)	1438
BspMI	ACCTGC	1 cut(s)	934
BspOI	GCTAGC	1 cut(s)	815
BspPI	GGATC	6 cut(s)	334, 347, 470, 616, 865, 1316
BspT104I	TTCGAA	1 cut(s)	1128
BspT107I	GGYRCC	3 cut(s)	611, 1234, 1419
BspTNI	GGTCTC	1 cut(s)	1313
BsrBI	CCGCTC	1 cut(s)	1432
BsrFI	RCCGGY	6 cut(s)	384, 408, 426, 470, 560, 689
BsrI	ACTGG	1 cut(s)	784
BssAI	RCCGGY	6 cut(s)	384, 408, 426, 470, 560, 689
BssECI	CCNNGG	3 cut(s)	70, 885, 1323
BssMI	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
BssNI	GRCGYC	3 cut(s)	510, 612, 783
BssSI	CACGAG	1 cut(s)	344
BssT1I	CCWWGG	1 cut(s)	1323
Bst2BI	CACGAG	1 cut(s)	344
Bst2UI	CCWGG	1 cut(s)	801
Bst4CI	ACNGT	7 cut(s)	146, 328, 635, 658, 1003, 1117, 1159
Bst6I	CTCTTC	1 cut(s)	1065
BstACI	GRCGYC	3 cut(s)	510, 612, 783
BstAPI	GCANNNNNTGC	1 cut(s)	1400
BstBI	TTCGAA	1 cut(s)	1128
BstDEI	CTNAG	3 cut(s)	188, 240, 1108
BstDSI	CCRYGG	1 cut(s)	885
BstEII	GGTNACC	2 cut(s)	422, 1441
BstENI	CCTNNNNNAGG	1 cut(s)	647
BstF5I	GGATG	5 cut(s)	349, 403, 739, 993, 1281
BstFNI	CGCG	1 cut(s)	1438
BstH2I	RGCGCY	2 cut(s)	203, 615
BstHHI	GCGC	3 cut(s)	202, 592, 614
BstKTI	GATC	8 cut(s)	115, 342, 465, 624, 714, 860, 982, 1311
BstMAI	GTCTC	3 cut(s)	89, 371, 1313
BstMBI	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
BstMCI	CGRYCG	3 cut(s)	302, 891, 1436
BstNI	CCWGG	1 cut(s)	801
BstNSI	RCATGY	2 cut(s)	596, 1187
BstPI	GGTNACC	2 cut(s)	422, 1441
BstSCI	CCNGG	1 cut(s)	799
BstSFI	CTRYAG	1 cut(s)	1048
BstSLI	GKGCMC	2 cut(s)	585, 1217
BstUI	CGCG	1 cut(s)	1438
BstV1I	GCAGC	5 cut(s)	143, 233, 764, 890, 1378
BstV2I	GAAGAC	1 cut(s)	329
BstX2I	RGATCY	3 cut(s)	339, 621, 857
BstXI	CCANNNNNNTGG	1 cut(s)	1003
BstYI	RGATCY	3 cut(s)	339, 621, 857
BstZI	CGGCCG	1 cut(s)	888
Bsu15I	ATCGAT	1 cut(s)	1203
BsuI	GTATCC	3 cut(s)	441, 772, 925
BsuRI	GGCC	6 cut(s)	743, 799, 890, 1199, 1215, 1344
BsuTUI	ATCGAT	1 cut(s)	1203
BtgI	CCRYGG	1 cut(s)	885
BtgZI	GCGATG	1 cut(s)	990
BtsCI	GGATG	5 cut(s)	349, 403, 739, 993, 1281
BtsIMutI	CAGTG	4 cut(s)	850, 999, 1122, 1164
BveI	ACCTGC	1 cut(s)	934
CfoI	GCGC	3 cut(s)	202, 592, 614
Cfr10I	RCCGGY	6 cut(s)	384, 408, 426, 470, 560, 689
Cfr13I	GGNCC	7 cut(s)	406, 476, 803, 1213, 1214, 1329, 1342
ClaI	ATCGAT	1 cut(s)	1203
CpoI	CGGWCCG	1 cut(s)	476
CseI	GACGC	2 cut(s)	518, 772
Csp6I	GTAC	3 cut(s)	1007, 1017, 1420
CspAI	ACCGGT	2 cut(s)	408, 426
CspI	CGGWCCG	1 cut(s)	476
CviAII	CATG	8 cut(s)	505, 593, 758, 767, 1178, 1184, 1243, 1400
CviQI	GTAC	3 cut(s)	1007, 1017, 1420
DdeI	CTNAG	3 cut(s)	188, 240, 1108
DinI	GGCGCC	1 cut(s)	613
DpnI	GATC	8 cut(s)	114, 341, 464, 623, 713, 859, 981, 1310
DpnII	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
DrdI	GACNNNNNNGTC	2 cut(s)	103, 1327
DseDI	GACNNNNNNGTC	2 cut(s)	103, 1327
EaeI	YGGCCR	2 cut(s)	888, 1197
EagI	CGGCCG	1 cut(s)	888
Eam1104I	CTCTTC	1 cut(s)	1065
EarI	CTCTTC	1 cut(s)	1065
EciI	GGCGGA	4 cut(s)	201, 349, 468, 1268
Ecl136II	GAGCTC	2 cut(s)	136, 358
EclXI	CGGCCG	1 cut(s)	888
Eco130I	CCWWGG	1 cut(s)	1323
Eco147I	AGGCCT	1 cut(s)	799
Eco24I	GRGCYC	3 cut(s)	138, 360, 1217
Eco31I	GGTCTC	1 cut(s)	1313
Eco32I	GATATC	1 cut(s)	418
Eco47I	GGWCC	4 cut(s)	406, 476, 803, 1329
Eco47III	AGCGCT	1 cut(s)	201
Eco52I	CGGCCG	1 cut(s)	888
Eco53kI	GAGCTC	2 cut(s)	136, 358
Eco57I	CTGAAG	4 cut(s)	647, 668, 814, 1210
Eco91I	GGTNACC	2 cut(s)	422, 1441
EcoICRI	GAGCTC	2 cut(s)	136, 358
EcoNI	CCTNNNNNAGG	1 cut(s)	647
EcoO109I	RGGNCCY	1 cut(s)	1214
EcoO65I	GGTNACC	2 cut(s)	422, 1441
EcoRI	GAATTC	1 cut(s)	1053
EcoRII	CCWGG	1 cut(s)	799
EcoRV	GATATC	1 cut(s)	418
EcoT14I	CCWWGG	1 cut(s)	1323
EcoT22I	ATGCAT	1 cut(s)	1179
EcoT38I	GRGCYC	3 cut(s)	138, 360, 1217
EgeI	GGCGCC	1 cut(s)	613
EheI	GGCGCC	1 cut(s)	613
ErhI	CCWWGG	1 cut(s)	1323
Esp3I	CGTCTC	2 cut(s)	89, 371
FaeI	CATG	8 cut(s)	508, 596, 761, 770, 1181, 1187, 1246, 1403
FaqI	GGGAC	1 cut(s)	789
FatI	CATG	8 cut(s)	504, 592, 757, 766, 1177, 1183, 1242, 1399
FauI	CCCGC	2 cut(s)	97, 1431
Fnu4HI	GCNGC	7 cut(s)	157, 247, 615, 753, 879, 882, 1392
FokI	GGATG	5 cut(s)	336, 410, 746, 1000, 1268
FriOI	GRGCYC	3 cut(s)	138, 360, 1217
Fsp4HI	GCNGC	7 cut(s)	157, 247, 615, 753, 879, 882, 1392
FspAI	RTGCGCAY	1 cut(s)	591
FspBI	CTAG	1 cut(s)	812
FspI	TGCGCA	1 cut(s)	591
GlaI	GCGC	3 cut(s)	201, 591, 613
GluI	GCNGC	7 cut(s)	157, 247, 615, 753, 879, 882, 1392
GsaI	CCCAGC	2 cut(s)	156, 1214
HaeII	RGCGCY	2 cut(s)	203, 615
HaeIII	GGCC	6 cut(s)	743, 799, 890, 1199, 1215, 1344
HapII	CCGG	9 cut(s)	108, 302, 385, 409, 427, 447, 471, 561, 690
HgaI	GACGC	2 cut(s)	518, 772
HhaI	GCGC	3 cut(s)	202, 592, 614
Hin1I	GRCGYC	3 cut(s)	510, 612, 783
Hin1II	CATG	8 cut(s)	508, 596, 761, 770, 1181, 1187, 1246, 1403
Hin6I	GCGC	3 cut(s)	200, 590, 612
HinP1I	GCGC	3 cut(s)	200, 590, 612
HinfI	GANTC	5 cut(s)	100, 184, 450, 923, 938
HpaII	CCGG	9 cut(s)	108, 302, 385, 409, 427, 447, 471, 561, 690
HphI	GGTGA	4 cut(s)	78, 213, 340, 1453
Hpy166II	GTNNAC	5 cut(s)	638, 1099, 1120, 1162, 1332
Hpy188I	TCNGA	8 cut(s)	189, 243, 455, 467, 648, 716, 833, 1276
Hpy188III	TCNNGA	7 cut(s)	353, 376, 395, 524, 625, 1306, 1312
Hpy8I	GTNNAC	5 cut(s)	638, 1099, 1120, 1162, 1332
Hpy99I	CGWCG	3 cut(s)	232, 235, 1370
HpyAV	CCTTC	3 cut(s)	524, 643, 653
HpyCH4III	ACNGT	7 cut(s)	146, 328, 635, 658, 1003, 1117, 1159
HpyCH4IV	ACGT	1 cut(s)	309
HpyCH4V	TGCA	9 cut(s)	159, 571, 701, 752, 904, 962, 1177, 1394, 1412
HpyF3I	CTNAG	3 cut(s)	188, 240, 1108
HpySE526I	ACGT	1 cut(s)	309
Hsp92I	GRCGYC	3 cut(s)	510, 612, 783
Hsp92II	CATG	8 cut(s)	508, 596, 761, 770, 1181, 1187, 1246, 1403
HspAI	GCGC	3 cut(s)	200, 590, 612
KasI	GGCGCC	1 cut(s)	611
KpnI	GGTACC	1 cut(s)	1423
KroI	GCCGGC	2 cut(s)	384, 560
KroNI	GCCGGC	2 cut(s)	386, 562
Kzo9I	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
LmnI	GCTCC	2 cut(s)	541, 1084
Lsp1109I	GCAGC	5 cut(s)	143, 233, 764, 890, 1378
LweI	GCATC	5 cut(s)	358, 577, 712, 918, 1094
MaeI	CTAG	1 cut(s)	812
MaeII	ACGT	1 cut(s)	309
MaeIII	GTNAC	4 cut(s)	422, 595, 955, 1441
MalI	GATC	8 cut(s)	114, 341, 464, 623, 713, 859, 981, 1310
MbiI	CCGCTC	1 cut(s)	1432
MboI	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
MfeI	CAATTG	2 cut(s)	1383, 1407
MflI	RGATCY	3 cut(s)	339, 621, 857
MhlI	GDGCHC	7 cut(s)	138, 272, 360, 546, 585, 774, 1217
MlsI	TGGCCA	1 cut(s)	1199
MluNI	TGGCCA	1 cut(s)	1199
Mly113I	GGCGCC	1 cut(s)	612
MlyI	GAGTC	2 cut(s)	94, 932
MmeI	TCCRAC	4 cut(s)	384, 393, 1059, 1178
Mox20I	TGGCCA	1 cut(s)	1199
Mph1103I	ATGCAT	1 cut(s)	1179
MroNI	GCCGGC	2 cut(s)	384, 560
MroXI	GAANNNNTTC	1 cut(s)	642
MscI	TGGCCA	1 cut(s)	1199
MseI	TTAA	4 cut(s)	9, 29, 50, 1462
MslI	CAYNNNNRTG	2 cut(s)	576, 1182
Msp20I	TGGCCA	1 cut(s)	1199
MspA1I	CMGCKG	2 cut(s)	156, 680
MspI	CCGG	9 cut(s)	108, 302, 385, 409, 427, 447, 471, 561, 690
MspR9I	CCNGG	1 cut(s)	801
MunI	CAATTG	2 cut(s)	1383, 1407
Mva1269I	GAATGC	1 cut(s)	962
MvaI	CCWGG	1 cut(s)	801
MvnI	CGCG	1 cut(s)	1438
NaeI	GCCGGC	2 cut(s)	386, 562
NarI	GGCGCC	1 cut(s)	612
NdeII	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
NgoMIV	GCCGGC	2 cut(s)	384, 560
NheI	GCTAGC	1 cut(s)	811
NlaIII	CATG	8 cut(s)	508, 596, 761, 770, 1181, 1187, 1246, 1403
NmeAIII	GCCGAG	1 cut(s)	118
NmuCI	GTSAC	2 cut(s)	595, 1441
NsbI	TGCGCA	1 cut(s)	591
NsiI	ATGCAT	1 cut(s)	1179
NspI	RCATGY	2 cut(s)	596, 1187
NspV	TTCGAA	1 cut(s)	1128
OliI	CACNNNNGTG	1 cut(s)	576
PceI	AGGCCT	1 cut(s)	799
PcsI	WCGNNNNNNNCGW	1 cut(s)	372
PctI	GAATGC	1 cut(s)	962
PdiI	GCCGGC	2 cut(s)	386, 562
PdmI	GAANNNNTTC	1 cut(s)	642
PfeI	GAWTC	3 cut(s)	184, 450, 923
PflFI	GACNNNGTC	1 cut(s)	103
PinAI	ACCGGT	2 cut(s)	408, 426
PkrI	GCNGC	7 cut(s)	158, 248, 616, 754, 880, 883, 1393
PleI	GAGTC	2 cut(s)	94, 932
PluTI	GGCGCC	1 cut(s)	615
PpsI	GAGTC	2 cut(s)	94, 932
PshBI	ATTAAT	2 cut(s)	29, 50
Psp124BI	GAGCTC	2 cut(s)	138, 360
Psp6I	CCWGG	1 cut(s)	799
PspEI	GGTNACC	2 cut(s)	422, 1441
PspFI	CCCAGC	2 cut(s)	152, 1210
PspGI	CCWGG	1 cut(s)	799
PspOMI	GGGCCC	1 cut(s)	1213
PspPI	GGNCC	7 cut(s)	406, 476, 803, 1213, 1214, 1329, 1342
PsrI	GAACNNNNNNTAC	2 cut(s)	1130, 1162
PsuI	RGATCY	3 cut(s)	339, 621, 857
PsyI	GACNNNGTC	1 cut(s)	103
PvuII	CAGCTG	1 cut(s)	156
RsaI	GTAC	3 cut(s)	1008, 1018, 1421
RsaNI	GTAC	3 cut(s)	1007, 1017, 1420
RseI	CAYNNNNRTG	2 cut(s)	576, 1182
Rsr2I	CGGWCCG	1 cut(s)	476
RsrII	CGGWCCG	1 cut(s)	476
SacI	GAGCTC	2 cut(s)	138, 360
SaqAI	TTAA	4 cut(s)	9, 29, 50, 1462
SatI	GCNGC	7 cut(s)	157, 247, 615, 753, 879, 882, 1392
Sau3AI	GATC	8 cut(s)	112, 339, 462, 621, 711, 857, 979, 1308
Sau96I	GGNCC	7 cut(s)	406, 476, 803, 1213, 1214, 1329, 1342
SchI	GAGTC	2 cut(s)	94, 932
ScrFI	CCNGG	1 cut(s)	801
SduI	GDGCHC	7 cut(s)	138, 272, 360, 546, 585, 774, 1217
SfaNI	GCATC	5 cut(s)	358, 577, 712, 918, 1094
SfcI	CTRYAG	1 cut(s)	1048
SfoI	GGCGCC	1 cut(s)	613
SfuI	TTCGAA	1 cut(s)	1128
SinI	GGWCC	4 cut(s)	406, 476, 803, 1329
SmiMI	CAYNNNNRTG	2 cut(s)	576, 1182
SseBI	AGGCCT	1 cut(s)	799
SspDI	GGCGCC	1 cut(s)	611
SspI	AATATT	2 cut(s)	48, 663
SspMI	CTAG	1 cut(s)	812
SstI	GAGCTC	2 cut(s)	138, 360
StuI	AGGCCT	1 cut(s)	799
StyD4I	CCNGG	1 cut(s)	799
StyI	CCWWGG	1 cut(s)	1323
TaaI	ACNGT	7 cut(s)	146, 328, 635, 658, 1003, 1117, 1159
TaiI	ACGT	1 cut(s)	312
TaqI	TCGA	9 cut(s)	77, 132, 138, 178, 366, 375, 1128, 1203, 1365
TauI	GCSGC	2 cut(s)	617, 884
TfiI	GAWTC	3 cut(s)	184, 450, 923
Tru1I	TTAA	4 cut(s)	9, 29, 50, 1462
Tru9I	TTAA	4 cut(s)	9, 29, 50, 1462
TscAI	CASTG	4 cut(s)	850, 1006, 1122, 1164
TseFI	GTSAC	2 cut(s)	595, 1441
TseI	GCWGC	5 cut(s)	156, 246, 752, 878, 1391
Tsp45I	GTSAC	2 cut(s)	595, 1441
TspDTI	ATGAA	2 cut(s)	17, 1351
TspGWI	ACGGA	2 cut(s)	93, 275
TspRI	CASTG	4 cut(s)	850, 1006, 1122, 1164
Tth111I	GACNNNGTC	1 cut(s)	103
VpaK11BI	GGWCC	4 cut(s)	406, 476, 803, 1329
VspI	ATTAAT	2 cut(s)	29, 50
XagI	CCTNNNNNAGG	1 cut(s)	647
XapI	RAATTY	3 cut(s)	4, 40, 1053
XceI	RCATGY	2 cut(s)	596, 1187
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1207, 1228
XmnI	GAANNNNTTC	1 cut(s)	642
XspI	CTAG	1 cut(s)	812
Zsp2I	ATGCAT	1 cut(s)	1179

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.