FvH4_2g27800

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015080
Physical Location & Seq
Forward (+)
21970415 .. 21971981
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27800.t1

Sequence Viewer

Length: 912 bp
ATGGCAATTCCGGTGATTGATTTCTCAAAACTCGATGGTGAGGAAAGGGCCGCCACATTGGCTCAGATTGCTAATGGATGTGAGGAATGGGGCTTCTTCCAGCTGGTGAACCATGGAATTTCAGAAGAGCTCCTGGAGAGGGTGAAGAAGGTGAGCGACGAGTTCTTTAAGAACGAAAGAGAAGAGAACTTCAAGAAGTCGACACTAGTGCAGTCGCTGAATGCGGACCAAAAGCTGGAGAATGTGGACTGGGAAGATGTCATTACCCTTCTTGATGACAATGAGTGGCCCTCGAAAACCCCTGGATTCAAGGAAACCATGGAAGAGTATAGAGCAGAACTGAAGAAACTAGCTGAGAGGGTAATGGAAGTGATGGATGAGAACTTGGGTTTACCAAAGGGACACATGAAGAAAGCCTTCAATGGTGGAGGAGTCGACGATGCGTTTTTCGGTACCAAGGTGAGCCACTACCCACCGTGTCCTCAACCCGAGTTGGTGACAGGTCTCCGAGCTCACACAGATGCTGGGGGTGTCATCTTGCTCTTCCAAGATGACCAGGTGGGAGGCCTCCAAATCCTCAAAGATGGGGAATGGATAGATGTGCAGCCACTGCGCAATTCGATTGTCATCAACACCGGAGACCAGATTGAAGTCCTGAGCAATGGCCTCTACAAGAGTGTTTGGCACCGGGTTTTGGCAACCCCGACCGGGAATAGAAGATCAATTGCTTCATTTTACAACCCATCACTGAAGGCCACCATTGCTCCTGCACCGGAATTGGTGGAGAAGGCAAACCAAGAAGTGGATCAGAGTACTTATCCCAAGTTTGTGTTTGGTGACTACTTGTCTATTTATGCTGAGCAGAAGTTCCTCCCCAAAGAACCAAGGTTCCAAGCTGTGAGGGCCATGTGA

Protein Analysis

304

Amino Acids

34.15

Weight (kDa)

4.93

Isoelectric Point (pI)

31.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 3 - 62 6.6e-20 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 154 - 248 5.6e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77330
fragaria_vesca FvH4_2g27800
malus_domestica MD08G1160900.v1.1
prunus_persica Prupe.1G490000_v2.0.a1
pyrus_communis pycom348g00080
rosa_chinensis RchiOBHm_Chr6g0296661
rosa_laevigata RLG00000011672
rosa_multiflora Rmu_sc0001741.1_g000013
rosa_roxburghii Rroxscaffold_7G00171280
rosa_rugosa Rorug06G0264000
rosa_samantha Rh6AG375800 Rh6BG384100 Rh6CG389200 Rh6DG376600
rosa_wichuraiana Rw6G032760 Rw6G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 614
Acc65I GGTACC 1 cut(s) 452
AccB1I GGYRCC 2 cut(s) 452, 684
AccB7I CCANNNNNTGG 2 cut(s) 235, 802
AccI GTMKAC 2 cut(s) 200, 435
AciI CCGC 2 cut(s) 51, 224
AclWI GGATC 1 cut(s) 813
AcsI RAATTY 1 cut(s) 117
AcuI CTGAAG 2 cut(s) 362, 770
AfaI GTAC 2 cut(s) 454, 814
AfiI CCNNNNNNNGG 5 cut(s) 139, 235, 694, 708, 802
AgsI TTSAA 4 cut(s) 193, 310, 421, 650
AhdI GACNNNNNGTC 1 cut(s) 844
AhlI ACTAGT 1 cut(s) 205
AjnI CCWGG 3 cut(s) 132, 301, 555
AloI GAACNNNNNNTCC 2 cut(s) 873, 905
AluBI AGCT 6 cut(s) 103, 130, 235, 353, 512, 896
AluI AGCT 6 cut(s) 103, 130, 235, 353, 512, 896
Alw21I GWGCWC 2 cut(s) 132, 514
Alw26I GTCTC 2 cut(s) 509, 633
AlwI GGATC 1 cut(s) 813
AlwNI CAGNNNCTG 3 cut(s) 217, 524, 610
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 6 cut(s) 48, 287, 565, 664, 753, 903
ApeKI GCWGC 1 cut(s) 604
ApoI RAATTY 1 cut(s) 117
Asp700I GAANNNNTTC 1 cut(s) 416
Asp718I GGTACC 1 cut(s) 452
AspLEI GCGC 1 cut(s) 615
AspS9I GGNCC 4 cut(s) 48, 226, 288, 903
AsuC2I CCSGG 2 cut(s) 689, 709
AsuHPI GGTGA 8 cut(s) 25, 50, 118, 154, 163, 472, 508, 848
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 1 cut(s) 226
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BanI GGYRCC 2 cut(s) 452, 684
BanII GRGCYC 2 cut(s) 132, 514
Bbv12I GWGCWC 2 cut(s) 132, 514
BbvI GCAGC 1 cut(s) 616
BccI CCATC 4 cut(s) 29, 367, 578, 751
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 3 cut(s) 134, 303, 557
BcnI CCSGG 2 cut(s) 689, 709
BcoDI GTCTC 2 cut(s) 509, 633
BcuI ACTAGT 1 cut(s) 205
BfaI CTAG 2 cut(s) 206, 350
BglI GCCNNNNNGGC 1 cut(s) 59
BisI GCNGC 2 cut(s) 51, 605
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 2 cut(s) 52, 606
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 5 cut(s) 134, 303, 557, 689, 709
Bme18I GGWCC 1 cut(s) 226
BmeRI GACNNNNNGTC 1 cut(s) 844
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 4 cut(s) 48, 226, 288, 903
BmiI GGNNCC 3 cut(s) 454, 686, 890
BmrFI CCNGG 5 cut(s) 134, 303, 557, 689, 709
BmrI ACTGGG 1 cut(s) 259
BmsI GCATC 2 cut(s) 430, 511
BmuI ACTGGG 1 cut(s) 259
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpmI CTGGAG 2 cut(s) 155, 257
Bpu10I CCTNAGC 1 cut(s) 656
Bpu1102I GCTNAGC 1 cut(s) 858
BpuMI CCSGG 2 cut(s) 689, 709
BsaBI GATNNNNATC 1 cut(s) 626
BsaI GGTCTC 2 cut(s) 509, 633
BsaJI CCNNGG 5 cut(s) 112, 301, 318, 456, 884
BsaWI WCCGGW 3 cut(s) 10, 635, 772
BsaXI ACNNNNNCTCC 2 cut(s) 748, 778
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 235, 694, 708, 802
Bse1I ACTGG 1 cut(s) 254
Bse3DI GCAATG 2 cut(s) 667, 759
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 3 cut(s) 134, 303, 557
BseDI CCNNGG 5 cut(s) 112, 301, 318, 456, 884
BseGI GGATG 2 cut(s) 83, 382
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 5 cut(s) 139, 235, 694, 708, 802
BseMI GCAATG 2 cut(s) 667, 759
BseMII CTCAG 4 cut(s) 77, 345, 647, 849
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 444
BseXI GCAGC 1 cut(s) 616
BseYI CCCAGC 1 cut(s) 524
BsgI GTGCAG 3 cut(s) 230, 623, 753
Bsh1285I CGRYCG 1 cut(s) 708
BshFI GGCC 6 cut(s) 50, 289, 567, 666, 755, 905
BshNI GGYRCC 2 cut(s) 452, 684
BsiEI CGRYCG 1 cut(s) 708
BsiHKAI GWGCWC 2 cut(s) 132, 514
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 5 cut(s) 11, 636, 688, 708, 773
BslFI GGGAC 1 cut(s) 414
BslI CCNNNNNNNGG 5 cut(s) 139, 235, 694, 708, 802
BsmAI GTCTC 2 cut(s) 509, 633
BsmFI GGGAC 1 cut(s) 414
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 6 cut(s) 50, 289, 567, 666, 755, 905
Bso31I GGTCTC 2 cut(s) 509, 633
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 132, 514
Bsp143I GATC 2 cut(s) 719, 805
Bsp1720I GCTNAGC 1 cut(s) 858
Bsp19I CCATGG 2 cut(s) 112, 318
BspACI CCGC 2 cut(s) 51, 224
BspANI GGCC 6 cut(s) 50, 289, 567, 666, 755, 905
BspCNI CTCAG 4 cut(s) 76, 346, 648, 850
BspLI GGNNCC 3 cut(s) 454, 686, 890
BspPI GGATC 1 cut(s) 813
BspQI GCTCTTC 2 cut(s) 120, 548
BspT107I GGYRCC 2 cut(s) 452, 684
BspTNI GGTCTC 2 cut(s) 509, 633
BsrDI GCAATG 2 cut(s) 667, 759
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 5 cut(s) 112, 301, 318, 456, 884
BssMI GATC 2 cut(s) 719, 805
BssT1I CCWWGG 4 cut(s) 112, 318, 456, 884
Bst2UI CCWGG 3 cut(s) 134, 303, 557
Bst4CI ACNGT 1 cut(s) 477
Bst6I CTCTTC 4 cut(s) 120, 177, 318, 548
BstAPI GCANNNNNTGC 1 cut(s) 610
BstDEI CTNAG 4 cut(s) 63, 354, 656, 858
BstDSI CCRYGG 2 cut(s) 112, 318
BstF5I GGATG 2 cut(s) 83, 382
BstHHI GCGC 1 cut(s) 615
BstKTI GATC 2 cut(s) 722, 808
BstMAI GTCTC 2 cut(s) 509, 633
BstMBI GATC 2 cut(s) 719, 805
BstMCI CGRYCG 1 cut(s) 708
BstMWI GCNNNNNNNGC 5 cut(s) 59, 68, 610, 761, 902
BstNI CCWGG 3 cut(s) 134, 303, 557
BstSCI CCNGG 5 cut(s) 132, 301, 555, 687, 707
BstV1I GCAGC 1 cut(s) 616
BsuRI GGCC 6 cut(s) 50, 289, 567, 666, 755, 905
BtgI CCRYGG 2 cut(s) 112, 318
BtsCI GGATG 2 cut(s) 83, 382
BtsI GCAGTG 1 cut(s) 608
BtsIMutI CAGTG 2 cut(s) 608, 746
CaiI CAGNNNCTG 3 cut(s) 217, 524, 610
CfoI GCGC 1 cut(s) 615
Cfr13I GGNCC 4 cut(s) 48, 226, 288, 903
CsiI ACCWGGT 1 cut(s) 555
Csp6I GTAC 2 cut(s) 453, 813
CviAII CATG 4 cut(s) 113, 319, 406, 907
CviQI GTAC 2 cut(s) 453, 813
DdeI CTNAG 4 cut(s) 63, 354, 656, 858
DpnI GATC 2 cut(s) 721, 807
DpnII GATC 2 cut(s) 719, 805
DriI GACNNNNNGTC 1 cut(s) 844
Eam1104I CTCTTC 4 cut(s) 120, 177, 318, 548
Eam1105I GACNNNNNGTC 1 cut(s) 844
EarI CTCTTC 4 cut(s) 120, 177, 318, 548
Ecl136II GAGCTC 2 cut(s) 130, 512
Eco130I CCWWGG 4 cut(s) 112, 318, 456, 884
Eco147I AGGCCT 1 cut(s) 567
Eco24I GRGCYC 2 cut(s) 132, 514
Eco31I GGTCTC 2 cut(s) 509, 633
Eco47I GGWCC 1 cut(s) 226
Eco53kI GAGCTC 2 cut(s) 130, 512
Eco57I CTGAAG 2 cut(s) 362, 770
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 2 cut(s) 130, 512
EcoRII CCWGG 3 cut(s) 132, 301, 555
EcoT14I CCWWGG 4 cut(s) 112, 318, 456, 884
EcoT38I GRGCYC 2 cut(s) 132, 514
ErhI CCWWGG 4 cut(s) 112, 318, 456, 884
FaeI CATG 4 cut(s) 116, 322, 409, 910
FaiI YATR 6 cut(s) 114, 320, 330, 407, 855, 908
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FaqI GGGAC 1 cut(s) 414
FatI CATG 4 cut(s) 112, 318, 405, 906
FblI GTMKAC 2 cut(s) 200, 435
Fnu4HI GCNGC 2 cut(s) 51, 605
FokI GGATG 2 cut(s) 90, 389
FriOI GRGCYC 2 cut(s) 132, 514
Fsp4HI GCNGC 2 cut(s) 51, 605
FspBI CTAG 2 cut(s) 206, 350
FspI TGCGCA 1 cut(s) 614
GlaI GCGC 1 cut(s) 614
GluI GCNGC 2 cut(s) 51, 605
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 2 cut(s) 155, 257
HaeIII GGCC 6 cut(s) 50, 289, 567, 666, 755, 905
HapII CCGG 5 cut(s) 11, 636, 688, 708, 773
HhaI GCGC 1 cut(s) 615
Hin1II CATG 4 cut(s) 116, 322, 409, 910
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HincII GTYRAC 2 cut(s) 201, 436
HindII GTYRAC 2 cut(s) 201, 436
HinfI GANTC 2 cut(s) 306, 432
HpaII CCGG 5 cut(s) 11, 636, 688, 708, 773
HphI GGTGA 8 cut(s) 25, 50, 118, 154, 163, 472, 508, 848
Hpy166II GTNNAC 5 cut(s) 109, 201, 247, 392, 436
Hpy188I TCNGA 4 cut(s) 66, 124, 509, 810
Hpy188III TCNNGA 3 cut(s) 193, 272, 655
Hpy8I GTNNAC 5 cut(s) 109, 201, 247, 392, 436
Hpy99I CGWCG 2 cut(s) 161, 440
HpyAV CCTTC 5 cut(s) 142, 278, 427, 745, 781
HpyCH4III ACNGT 1 cut(s) 477
HpyCH4V TGCA 3 cut(s) 211, 604, 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 68, 610, 761, 902
HpyF3I CTNAG 4 cut(s) 63, 354, 656, 858
Hsp92II CATG 4 cut(s) 116, 322, 409, 910
HspAI GCGC 1 cut(s) 613
KpnI GGTACC 1 cut(s) 456
Kzo9I GATC 2 cut(s) 719, 805
LguI GCTCTTC 2 cut(s) 120, 548
LmnI GCTCC 2 cut(s) 135, 769
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 2 cut(s) 430, 511
MabI ACCWGGT 1 cut(s) 555
MaeI CTAG 2 cut(s) 206, 350
MaeIII GTNAC 2 cut(s) 496, 836
MalI GATC 2 cut(s) 721, 807
MboI GATC 2 cut(s) 719, 805
MfeI CAATTG 1 cut(s) 723
MhlI GDGCHC 2 cut(s) 132, 514
MluCI AATT 5 cut(s) 6, 117, 616, 723, 776
MlyI GAGTC 1 cut(s) 441
MroXI GAANNNNTTC 1 cut(s) 416
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 5 cut(s) 11, 636, 688, 708, 773
MspR9I CCNGG 5 cut(s) 134, 303, 557, 689, 709
MunI CAATTG 1 cut(s) 723
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 3 cut(s) 134, 303, 557
MwoI GCNNNNNNNGC 5 cut(s) 59, 68, 610, 761, 902
NciI CCSGG 2 cut(s) 689, 709
NcoI CCATGG 2 cut(s) 112, 318
NdeII GATC 2 cut(s) 719, 805
NlaIII CATG 4 cut(s) 116, 322, 409, 910
NlaIV GGNNCC 3 cut(s) 454, 686, 890
NmuCI GTSAC 2 cut(s) 496, 836
NsbI TGCGCA 1 cut(s) 614
PceI AGGCCT 1 cut(s) 567
PciSI GCTCTTC 2 cut(s) 120, 548
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 1 cut(s) 306
PflMI CCANNNNNTGG 2 cut(s) 235, 802
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 2 cut(s) 52, 606
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Psp124BI GAGCTC 2 cut(s) 132, 514
Psp6I CCWGG 3 cut(s) 132, 301, 555
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 3 cut(s) 132, 301, 555
PspN4I GGNNCC 3 cut(s) 454, 686, 890
PspPI GGNCC 4 cut(s) 48, 226, 288, 903
PstNI CAGNNNCTG 3 cut(s) 217, 524, 610
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 2 cut(s) 454, 814
RsaNI GTAC 2 cut(s) 453, 813
RseI CAYNNNNRTG 1 cut(s) 519
SacI GAGCTC 2 cut(s) 132, 514
SalI GTCGAC 2 cut(s) 199, 434
SapI GCTCTTC 2 cut(s) 120, 548
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 2 cut(s) 51, 605
Sau3AI GATC 2 cut(s) 719, 805
Sau96I GGNCC 4 cut(s) 48, 226, 288, 903
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 1 cut(s) 441
ScrFI CCNGG 5 cut(s) 134, 303, 557, 689, 709
SduI GDGCHC 2 cut(s) 132, 514
SexAI ACCWGGT 1 cut(s) 555
SfaNI GCATC 2 cut(s) 430, 511
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 519
SpeI ACTAGT 1 cut(s) 205
Sse9I AATT 5 cut(s) 6, 117, 616, 723, 776
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 2 cut(s) 51, 224
SspMI CTAG 2 cut(s) 206, 350
SstI GAGCTC 2 cut(s) 132, 514
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 5 cut(s) 132, 301, 555, 687, 707
StyI CCWWGG 4 cut(s) 112, 318, 456, 884
TaaI ACNGT 1 cut(s) 477
TaqI TCGA 5 cut(s) 33, 200, 293, 435, 620
TasI AATT 5 cut(s) 6, 117, 616, 723, 776
TatI WGTACW 1 cut(s) 812
TauI GCSGC 1 cut(s) 53
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 2 cut(s) 615, 753
TseFI GTSAC 2 cut(s) 496, 836
TseI GCWGC 1 cut(s) 604
Tsp45I GTSAC 2 cut(s) 496, 836
TspDTI ATGAA 2 cut(s) 422, 720
TspRI CASTG 2 cut(s) 615, 753
Van91I CCANNNNNTGG 2 cut(s) 235, 802
VpaK11BI GGWCC 1 cut(s) 226
XapI RAATTY 1 cut(s) 117
XmiI GTMKAC 2 cut(s) 200, 435
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 2 cut(s) 206, 350
ZrmI AGTACT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.