Rw6G032760

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015080
Physical Location & Seq
Forward (+)
57012685 .. 57013867
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G032760.1

Sequence Viewer

Length: 909 bp
ATGGCAATTCCAGTGATTGATTTCTCAAAACTCGATGGTGAGGAAAGGGCCACCACATTGGCCCAGATTGCAAATGGATGTGAGCAATGGGGCTTCTTCCAGCTGGTGAACCATGGAATATCAGAAGACCTCCTGGAGAGGGTGAAGAAGGTGAGCGACGACTGCTTTAAGAACGAAAGAGAAGAGAACTTCAAGAAGTCGACACTGGTGCAGTCGCTGAATGTGGACCAAAAGCTGGAGAATGTGGACTGGGAAGATGTCTTTACCCTTCTTGATGACAATGAATGGCCCTCGAAAACCCCTGGATTCAAGGAAACCATGGCAGAGTACAGAGCAGAACTGAAGAAACTAGCCGAGAGGGTCATGGAAGTGATGGATGAGAACTTGGAATTACCAAAGGGATACATGAAGAAAGCCTTCAATGGTGGAGAAGGTGACAGTGCCTTTTTCGGCACCAAGGTGAGCCACTACCCGCCCTGTCCTCAACCCGAGTTGGTGACAGGTCTCCGAGCTCACACAGATGCCGGGGGTGTCATCTTGCTCTTCCAAGATGACCAGGTGGGAGGCCTACAAATCCTCAAAGATGGAGAATGGATAGATGTGCAGCCACTGCCCAATTCGATTGTCATCAACACTGGAGACCAGATTGAAGTCTTGAGCAATGGCCTCTACAAGAGTGTTTGGCACCGGGTTCTGGCCACCCGGACTGGGAACAGAAGATCAATTGCTTCATTTTACAACCCATCACTGAAGGCCACCATTGCTCCTGCACCAGAACTGGTGGAGAAAGCAAACCAAGAAGTGGATCAGACTTATCCCAAGTTTGTGTTTGGTGACTACCTGTCTGTTTATGCTGAGCAGAAGTTCCTCCCCAAGGAACCAAGGTTCCAAGCTGTGAGAGCCATCTGA

Protein Analysis

302

Amino Acids

34.1

Weight (kDa)

4.92

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 3 - 92 1.3e-17 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 154 - 248 1e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G77330
fragaria_vesca FvH4_2g27800
malus_domestica MD08G1160900.v1.1
prunus_persica Prupe.1G490000_v2.0.a1
pyrus_communis pycom348g00080
rosa_chinensis RchiOBHm_Chr6g0296661
rosa_laevigata RLG00000011672
rosa_multiflora Rmu_sc0001741.1_g000013
rosa_roxburghii Rroxscaffold_7G00171280
rosa_rugosa Rorug06G0264000
rosa_samantha Rh6AG375800 Rh6BG384100 Rh6CG389200 Rh6DG376600
rosa_wichuraiana Rw6G032760 Rw6G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 452, 684
AccB7I CCANNNNNTGG 2 cut(s) 235, 802
AccI GTMKAC 1 cut(s) 200
AciI CCGC 1 cut(s) 473
AclWI GGATC 1 cut(s) 813
AcoI YGGCCR 1 cut(s) 696
AcuI CTGAAG 2 cut(s) 362, 770
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 6 cut(s) 139, 235, 694, 708, 802, 874
AgsI TTSAA 4 cut(s) 193, 310, 421, 650
AhdI GACNNNNNGTC 1 cut(s) 841
AjnI CCWGG 3 cut(s) 132, 301, 555
AleI CACNNNNGTG 1 cut(s) 458
AloI GAACNNNNNNTCC 4 cut(s) 869, 870, 901, 902
AluBI AGCT 4 cut(s) 103, 235, 512, 893
AluI AGCT 4 cut(s) 103, 235, 512, 893
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 2 cut(s) 509, 633
AlwI GGATC 1 cut(s) 813
AlwNI CAGNNNCTG 2 cut(s) 217, 610
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 7 cut(s) 48, 60, 287, 565, 664, 696, 753
ApeKI GCWGC 1 cut(s) 604
Asp700I GAANNNNTTC 1 cut(s) 416
AspS9I GGNCC 4 cut(s) 48, 61, 226, 288
AsuC2I CCSGG 3 cut(s) 526, 689, 703
AsuHPI GGTGA 8 cut(s) 50, 118, 154, 163, 446, 472, 508, 845
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 1 cut(s) 226
BalI TGGCCA 1 cut(s) 698
BanI GGYRCC 2 cut(s) 452, 684
BanII GRGCYC 1 cut(s) 514
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 1 cut(s) 616
BccI CCATC 4 cut(s) 29, 367, 578, 751
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 3 cut(s) 134, 303, 557
BciVI GTATCC 1 cut(s) 395
BcnI CCSGG 3 cut(s) 526, 689, 703
BcoDI GTCTC 2 cut(s) 509, 633
BfaI CTAG 1 cut(s) 350
BfuI GTATCC 1 cut(s) 395
BisI GCNGC 1 cut(s) 605
BlpI GCTNAGC 1 cut(s) 855
BlsI GCNGC 1 cut(s) 606
Bme1390I CCNGG 6 cut(s) 134, 303, 526, 557, 689, 703
Bme18I GGWCC 1 cut(s) 226
BmeRI GACNNNNNGTC 1 cut(s) 841
BmeT110I CYCGRG 1 cut(s) 488
BmgT120I GGNCC 4 cut(s) 48, 61, 226, 288
BmiI GGNNCC 4 cut(s) 454, 686, 879, 887
BmrFI CCNGG 6 cut(s) 134, 303, 526, 557, 689, 703
BmrI ACTGGG 2 cut(s) 259, 717
BmsI GCATC 1 cut(s) 511
BmuI ACTGGG 2 cut(s) 259, 717
BpiI GAAGAC 1 cut(s) 132
BpmI CTGGAG 3 cut(s) 155, 257, 657
Bpu1102I GCTNAGC 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 676
BpuMI CCSGG 3 cut(s) 526, 689, 703
BsaBI GATNNNNATC 1 cut(s) 626
BsaI GGTCTC 2 cut(s) 509, 633
BsaJI CCNNGG 7 cut(s) 112, 301, 318, 456, 525, 873, 881
BsaXI ACNNNNNCTCC 2 cut(s) 748, 778
Bsc4I CCNNNNNNNGG 6 cut(s) 139, 235, 694, 708, 802, 874
Bse1I ACTGG 6 cut(s) 11, 210, 254, 640, 712, 783
Bse3DI GCAATG 3 cut(s) 92, 667, 759
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 3 cut(s) 134, 303, 557
BseDI CCNNGG 7 cut(s) 112, 301, 318, 456, 525, 873, 881
BseGI GGATG 2 cut(s) 83, 382
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 6 cut(s) 139, 235, 694, 708, 802, 874
BseMI GCAATG 3 cut(s) 92, 667, 759
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 6 cut(s) 11, 210, 254, 640, 712, 783
BseXI GCAGC 1 cut(s) 616
BsgI GTGCAG 3 cut(s) 230, 623, 753
BshFI GGCC 7 cut(s) 50, 62, 289, 567, 666, 698, 755
BshNI GGYRCC 2 cut(s) 452, 684
BsiHKAI GWGCWC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 525, 688, 703
BslI CCNNNNNNNGG 6 cut(s) 139, 235, 694, 708, 802, 874
BsmAI GTCTC 2 cut(s) 509, 633
BsnI GGCC 7 cut(s) 50, 62, 289, 567, 666, 698, 755
Bso31I GGTCTC 2 cut(s) 509, 633
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 2 cut(s) 719, 805
Bsp1720I GCTNAGC 1 cut(s) 855
Bsp19I CCATGG 2 cut(s) 112, 318
BspACI CCGC 1 cut(s) 473
BspANI GGCC 7 cut(s) 50, 62, 289, 567, 666, 698, 755
BspCNI CTCAG 1 cut(s) 847
BspLI GGNNCC 4 cut(s) 454, 686, 879, 887
BspPI GGATC 1 cut(s) 813
BspQI GCTCTTC 1 cut(s) 548
BspT107I GGYRCC 2 cut(s) 452, 684
BspTNI GGTCTC 2 cut(s) 509, 633
BsrDI GCAATG 3 cut(s) 92, 667, 759
BsrI ACTGG 6 cut(s) 11, 210, 254, 640, 712, 783
BssECI CCNNGG 7 cut(s) 112, 301, 318, 456, 525, 873, 881
BssMI GATC 2 cut(s) 719, 805
BssT1I CCWWGG 5 cut(s) 112, 318, 456, 873, 881
Bst2UI CCWGG 3 cut(s) 134, 303, 557
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 2 cut(s) 177, 548
BstAPI GCANNNNNTGC 1 cut(s) 610
BstDEI CTNAG 1 cut(s) 855
BstDSI CCRYGG 2 cut(s) 112, 318
BstENI CCTNNNNNAGG 1 cut(s) 872
BstF5I GGATG 2 cut(s) 83, 382
BstKTI GATC 2 cut(s) 722, 808
BstMAI GTCTC 2 cut(s) 509, 633
BstMBI GATC 2 cut(s) 719, 805
BstMWI GCNNNNNNNGC 5 cut(s) 68, 162, 610, 761, 899
BstNI CCWGG 3 cut(s) 134, 303, 557
BstSCI CCNGG 6 cut(s) 132, 301, 524, 555, 687, 701
BstV1I GCAGC 1 cut(s) 616
BstV2I GAAGAC 1 cut(s) 132
BstXI CCANNNNNNTGG 1 cut(s) 58
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 7 cut(s) 50, 62, 289, 567, 666, 698, 755
BtgI CCRYGG 2 cut(s) 112, 318
BtsCI GGATG 2 cut(s) 83, 382
BtsI GCAGTG 1 cut(s) 608
BtsIMutI CAGTG 6 cut(s) 18, 203, 445, 608, 633, 746
CaiI CAGNNNCTG 2 cut(s) 217, 610
Cfr13I GGNCC 4 cut(s) 48, 61, 226, 288
CsiI ACCWGGT 1 cut(s) 555
Csp6I GTAC 1 cut(s) 328
CviAII CATG 4 cut(s) 113, 319, 364, 406
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 1 cut(s) 855
DpnI GATC 2 cut(s) 721, 807
DpnII GATC 2 cut(s) 719, 805
DriI GACNNNNNGTC 1 cut(s) 841
EaeI YGGCCR 1 cut(s) 696
Eam1104I CTCTTC 2 cut(s) 177, 548
Eam1105I GACNNNNNGTC 1 cut(s) 841
EarI CTCTTC 2 cut(s) 177, 548
Ecl136II GAGCTC 1 cut(s) 512
Eco130I CCWWGG 5 cut(s) 112, 318, 456, 873, 881
Eco147I AGGCCT 1 cut(s) 567
Eco24I GRGCYC 1 cut(s) 514
Eco31I GGTCTC 2 cut(s) 509, 633
Eco47I GGWCC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 512
Eco57I CTGAAG 2 cut(s) 362, 770
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 512
EcoNI CCTNNNNNAGG 1 cut(s) 872
EcoRII CCWGG 3 cut(s) 132, 301, 555
EcoT14I CCWWGG 5 cut(s) 112, 318, 456, 873, 881
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 5 cut(s) 112, 318, 456, 873, 881
FaeI CATG 4 cut(s) 116, 322, 367, 409
FaiI YATR 5 cut(s) 114, 320, 365, 407, 852
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FatI CATG 4 cut(s) 112, 318, 363, 405
FauI CCCGC 1 cut(s) 480
FblI GTMKAC 1 cut(s) 200
Fnu4HI GCNGC 1 cut(s) 605
FokI GGATG 2 cut(s) 90, 389
FriOI GRGCYC 1 cut(s) 514
Fsp4HI GCNGC 1 cut(s) 605
FspBI CTAG 1 cut(s) 350
GluI GCNGC 1 cut(s) 605
GsuI CTGGAG 3 cut(s) 155, 257, 657
HaeIII GGCC 7 cut(s) 50, 62, 289, 567, 666, 698, 755
HapII CCGG 3 cut(s) 525, 688, 703
Hin1II CATG 4 cut(s) 116, 322, 367, 409
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 1 cut(s) 306
HpaII CCGG 3 cut(s) 525, 688, 703
HphI GGTGA 8 cut(s) 50, 118, 154, 163, 446, 472, 508, 845
Hpy166II GTNNAC 4 cut(s) 109, 201, 226, 247
Hpy188I TCNGA 4 cut(s) 124, 509, 810, 908
Hpy188III TCNNGA 3 cut(s) 193, 272, 655
Hpy8I GTNNAC 4 cut(s) 109, 201, 226, 247
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 5 cut(s) 142, 278, 425, 427, 745
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4V TGCA 4 cut(s) 71, 211, 604, 770
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 162, 610, 761, 899
HpyF3I CTNAG 1 cut(s) 855
Hsp92II CATG 4 cut(s) 116, 322, 367, 409
Kzo9I GATC 2 cut(s) 719, 805
LguI GCTCTTC 1 cut(s) 548
LmnI GCTCC 1 cut(s) 769
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 1 cut(s) 511
MabI ACCWGGT 1 cut(s) 555
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 3 cut(s) 434, 496, 833
MalI GATC 2 cut(s) 721, 807
MboI GATC 2 cut(s) 719, 805
MboII GAAGA 9 cut(s) 88, 137, 157, 194, 266, 355, 421, 535, 729
MfeI CAATTG 1 cut(s) 723
MhlI GDGCHC 1 cut(s) 514
MlsI TGGCCA 1 cut(s) 698
MluCI AATT 4 cut(s) 6, 389, 616, 723
MluNI TGGCCA 1 cut(s) 698
Mox20I TGGCCA 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 416
MscI TGGCCA 1 cut(s) 698
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 3 cut(s) 368, 458, 519
Msp20I TGGCCA 1 cut(s) 698
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 3 cut(s) 525, 688, 703
MspR9I CCNGG 6 cut(s) 134, 303, 526, 557, 689, 703
MunI CAATTG 1 cut(s) 723
MvaI CCWGG 3 cut(s) 134, 303, 557
MwoI GCNNNNNNNGC 5 cut(s) 68, 162, 610, 761, 899
NciI CCSGG 3 cut(s) 526, 689, 703
NcoI CCATGG 2 cut(s) 112, 318
NdeII GATC 2 cut(s) 719, 805
NlaIII CATG 4 cut(s) 116, 322, 367, 409
NlaIV GGNNCC 4 cut(s) 454, 686, 879, 887
NmeAIII GCCGAG 1 cut(s) 379
NmuCI GTSAC 3 cut(s) 434, 496, 833
OliI CACNNNNGTG 1 cut(s) 458
PceI AGGCCT 1 cut(s) 567
PciSI GCTCTTC 1 cut(s) 548
PdmI GAANNNNTTC 1 cut(s) 416
PfeI GAWTC 1 cut(s) 306
PflMI CCANNNNNTGG 2 cut(s) 235, 802
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 1 cut(s) 606
Psp124BI GAGCTC 1 cut(s) 514
Psp6I CCWGG 3 cut(s) 132, 301, 555
PspGI CCWGG 3 cut(s) 132, 301, 555
PspN4I GGNNCC 4 cut(s) 454, 686, 879, 887
PspPI GGNCC 4 cut(s) 48, 61, 226, 288
PstNI CAGNNNCTG 2 cut(s) 217, 610
PvuII CAGCTG 1 cut(s) 103
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
RseI CAYNNNNRTG 3 cut(s) 368, 458, 519
SacI GAGCTC 1 cut(s) 514
SalI GTCGAC 1 cut(s) 199
SapI GCTCTTC 1 cut(s) 548
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 1 cut(s) 605
Sau3AI GATC 2 cut(s) 719, 805
Sau96I GGNCC 4 cut(s) 48, 61, 226, 288
ScrFI CCNGG 6 cut(s) 134, 303, 526, 557, 689, 703
SduI GDGCHC 1 cut(s) 514
SexAI ACCWGGT 1 cut(s) 555
SfaNI GCATC 1 cut(s) 511
SinI GGWCC 1 cut(s) 226
SmiMI CAYNNNNRTG 3 cut(s) 368, 458, 519
SmlI CTYRAG 1 cut(s) 655
SmoI CTYRAG 1 cut(s) 655
Sse9I AATT 4 cut(s) 6, 389, 616, 723
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 1 cut(s) 473
SspMI CTAG 1 cut(s) 350
SstI GAGCTC 1 cut(s) 514
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 6 cut(s) 132, 301, 524, 555, 687, 701
StyI CCWWGG 5 cut(s) 112, 318, 456, 873, 881
TaaI ACNGT 1 cut(s) 440
TaqI TCGA 4 cut(s) 33, 200, 293, 620
TasI AATT 4 cut(s) 6, 389, 616, 723
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 6 cut(s) 18, 210, 445, 615, 640, 753
TseFI GTSAC 3 cut(s) 434, 496, 833
TseI GCWGC 1 cut(s) 604
Tsp45I GTSAC 3 cut(s) 434, 496, 833
TspDTI ATGAA 3 cut(s) 297, 422, 720
TspRI CASTG 6 cut(s) 18, 210, 445, 615, 640, 753
Van91I CCANNNNNTGG 2 cut(s) 235, 802
VpaK11BI GGWCC 1 cut(s) 226
XagI CCTNNNNNAGG 1 cut(s) 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 200
XmnI GAANNNNTTC 1 cut(s) 416
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.