Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0009089

Physical Location & Seq

Genomic Coordinates Reverse (-)

23838188 .. 23845929

Gene Structure 7742 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g30930.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGAACACTATTCTCATCAGAGCCTCACTCTCGCCAGACCCGAAGCTCTTGTCCACCAATTCCAATGTCGACCGCTTCGCCTTCAACAACTTCAAGCCCCACCTCCCCCAACGCCGTCGTTTCCCTCATCGCCGTCACCGTTTTCTCCTCCGCCACCTGTCCAAACCTAATTTCACTCTCTCTAGCGGCCTGCCGAATCCGATCGCGGCGGCGGTTCAGGAGGAGCCGCGCGCCGCCGCCGCGGAAGCGTCGGTGCTGCTCGACGTCTCCGGGATGATGTGTGGCGGCTGCGTGTCGCGAGTCAAGTCGGTGCTGTCTGCTGATGACCGAGTCCACTCTGTGGCGGTCAACATGTTGACCGAGACGGCGGCGGTTAAGCTGAAGGCGGAGGTAGGCGCGGAGGAGGCGGCGGAGAGCTTGGCGGGGAGGTTGACGGAGTGCGGATTCGCGGCGAAGAGGAGGGCGTCCGGGATGGGTGTGGCGGAGAGTGTGAGGAAGTGGAAGGAGATGGTGAAGAACAAAGAGGAGATGCTGGTCAAGAGTAGGAACCGTGTCATTTTGGCTTGGACTCTCGTGGCCTTGTGCTGTGGTTCACATGCTTCTCACATTTTGCATTCTCTGGGAATTCACATTGCTCATGGATCGTATATGGACGTGCTCCATAATTCGTATGTGAAGGGTGGTTTGGCAATGGCAGCTTTGTTGGGACCAGGACGAGACTTGCTTTTTGATGGTTTGAGGGCTTTTAGAAAAGGATCGCCCAATATGAATTCTCTTGTGGGATTTGGTTCCCTGGCTGCTTTTACAATCAGTGCGGTCTCTCTTCTTAACCCTGACCTTCAATGGGATGCAGCCTTCTTTGATGAGCCGGTCATGCTTCTTGGTTTTGTGCTCCTAGGACGTTCTCTTGAAGAAAGGGCAAGGATCAGGGCATCTAGTGATATGAATGAACTCTTGTCACTGATAAACACTCAATCAAGACTTGTAATCGCGTCCTCAGAAAATGATTCCTCCAGCGACACTGTACTCGGCTCCGATGCAATTTGCCTTGAGGTCCCAACTGATGATGTTCGTGTGGGAGATTCTGTGTTAGTATTGCCAGGAGAAACTATACCCGTGGATGGAAGAGTTCTGGCCGGAAGAAGTGTTGTGGATGAGTCCATGCTCACAGGAGAATCACTTCCTGTATTTAAGGAAAAAGAACTTACTGTCTCAGCCGGAACAATAAATTGGGATGGTCCTCTGAGGATTGAAGCAACTTCAACTGGCTCCAATTCAATGATTTCCAAGATTGTTCGCATGGTTGAGGATGCTCAAGGACATGAAGCACCAATACAAAGGCTTGCTGATTCAATAGCCGGACCTTTTGTATATACTATAATGACTCTCTCAGCTACAACATTTGCTTTTTGGTACTATATTGGAACTCATATTTTTCCAGATGTTTTGCTCAATGATATTGCTGGACCAGATGGAGATCCATTGCTGTTGAGCTTGAAACTAGCTGTGGATGTCCTGGTAGTTTCTTGTCCTTGTGCACTGGGTCTTGCGACACCCACAGCAATCCTAGTAGGCACTTCTCTTGGAGCTAGGCAAGGACTCCTTGTGAGAGGAGCAGATGTATTGGAACGCTTGGCCAGTATAGATCATATTGCTTTAGACAAGACAGGAACCCTCACGGAAGGAAAACCTGCTGTTTCTAGCATTGCATCTTTCAAGTATAAGGAGTCCGAAATTCTTCAAATTGCTGCTGCTGTAGAGAGTACAGCATCACACCCAATTGCTAATGCTATCTTAAACAAAGCCAAATCATTGGACTTGAGTATCCCAGTCACAAAAAGGCAATTAACAGAACCAGGATTTGGAACATTAGCAGAAGTAGATGGCCTTTTGGTTGCCGTGGGTTCTTTGGAATGGGTTCATGAGCGTTTCCAGAGAAGAACAGACCGATCTGAAATTTTGAACCTAGAGCATGCCGTATGTCGATCATCAGAAGGCATAACACCCTCAAGTTATTCAAAAACAATTGTATATGTTGGACGTGAAGGGGAAGGTATCATTGGTGCTATTGCAATATCCGACAGCCTCCGGCATGATGCTGAGTTTACTGTAACTAGACTCCAGCAGAAGGGTATCAAAACTGTCCTGTTTTCCGGGGACAGGGAAGAGGCAGTTGCAACTATAGCAAAAGCAGTTGGAATAGAAAAGAAATTTATCAAGTCATCATTGACTCCACAGGGGAAATCTGGAGCTATTTCTAGTCTTAAAGCGGCAGGGCATCATGTTGCAATGGTTGGTGATGGCATAAATGATGCACCTTCTTTGGCTCTTGCGGACGTTGGGATTGCTCTTCAGATTGGGGGACAAGAAAATGCTGCTTCAAATGCTGCATCGATTATACTTCTTGGAAACAAATTATCACAAGTTGTAGATGCACTGGAACTTGCACAGGCAACAATGGCAAAAGTGTATCAAAATTTATCTTGGGCTGTTGCATATAACGTAATTGCCATCCCCATAGCTGCCGGAGTACTGCTTCCGCAATACGATTTTGCCATGACACCATCACTTTCTGGTGGAATGATGGCCTTGAGCTCGATTTTTGTAGTAACCAACTCACTGCTTCTACAGCTTCATAAATCCGAAAGTGCAAGGAAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0035434 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

888

Amino Acids

94.48

Weight (kDa)

6.42

Isoelectric Point (pI)

35.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	87 - 148	2.3e-10	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	349 - 436	1.6e-27	P-type ATPase actuator domain
Hydrolase	PF00702	548 - 779	6.1e-25	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21930 AT5G21930 AT5G21930 AT5G21930
fragaria_vesca	FvH4_2g30930 FvH4_2g30930 FvH4_2g30930
malus_domestica	MD15G1049400.v1.1
prunus_persica	Prupe.1G403700_v2.0.a1 Prupe.1G403700_v2.0.a1
pyrus_communis	pycom15g04820
rosa_chinensis	RchiOBHm_Chr6g0311921
rosa_multiflora	Rmu_ssc0000057.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157220
rosa_rugosa	Rorug06G0391300 Rorug06G0391400 Rorug06G0391400
rosa_samantha	Rh6AG507800 Rh6BG519000 Rh6CG524700 Rh6DG510300
rosa_wichuraiana	Rw6G044200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	267
Acc36I	ACCTGC	1 cut(s)	1699
AccB7I	CCANNNNNTGG	2 cut(s)	340, 1862
AccI	GTMKAC	1 cut(s)	69
AccII	CGCG	8 cut(s)	206, 229, 231, 242, 298, 398, 449, 992
AclWI	GGATC	4 cut(s)	649, 763, 932, 1472
AcoI	YGGCCR	2 cut(s)	1134, 1635
AcsI	RAATTY	6 cut(s)	624, 769, 1734, 1956, 2210, 2476
AcuI	CTGAAG	2 cut(s)	401, 2336
AcyI	GRCGYC	2 cut(s)	264, 464
AdeI	CACNNNGTG	1 cut(s)	340
AfaI	GTAC	4 cut(s)	1026, 1415, 1765, 2532
AfiI	CCNNNNNNNGG	6 cut(s)	340, 844, 1121, 1862, 2128, 2160
AflIII	ACRYGT	1 cut(s)	351
AjiI	CACGTC	2 cut(s)	655, 2042
AjnI	CCWGG	5 cut(s)	709, 792, 1099, 1515, 1855
Alw21I	GWGCWC	4 cut(s)	660, 894, 1540, 2597
Alw26I	GTCTC	5 cut(s)	271, 356, 711, 823, 1216
Alw44I	GTGCAC	1 cut(s)	1536
AlwI	GGATC	4 cut(s)	649, 763, 932, 1472
AoxI	GGCC	6 cut(s)	187, 576, 1134, 1635, 1885, 2586
ApaLI	GTGCAC	1 cut(s)	1536
ApoI	RAATTY	6 cut(s)	624, 769, 1734, 1956, 2210, 2476
ArsI	GACNNNNNNTTYG	4 cut(s)	35, 67, 710, 742
Asp700I	GAANNNNTTC	3 cut(s)	1179, 1737, 1917
AspA2I	CCTAGG	1 cut(s)	895
AspLEI	GCGC	3 cut(s)	231, 233, 398
AspS9I	GGNCC	5 cut(s)	707, 1054, 1238, 1361, 1466
AsuC2I	CCSGG	3 cut(s)	271, 469, 2155
AsuHPI	GGTGA	3 cut(s)	128, 523, 2309
AvaII	GGWCC	5 cut(s)	707, 1054, 1238, 1361, 1466
AvrII	CCTAGG	1 cut(s)	895
BaeGI	GKGCMC	1 cut(s)	1540
BaeI	ACNNNNGTAYC	2 cut(s)	1807, 1840
BalI	TGGCCA	1 cut(s)	1637
BanII	GRGCYC	1 cut(s)	2597
BarI	GAAGNNNNNNTAC	2 cut(s)	1317, 1349
BauI	CACGAG	1 cut(s)	572
Bbv12I	GWGCWC	4 cut(s)	660, 894, 1540, 2597
BceAI	ACGGC	5 cut(s)	99, 117, 381, 1883, 1961
BciT130I	CCWGG	5 cut(s)	711, 794, 1101, 1517, 1857
BciVI	GTATCC	1 cut(s)	1835
BcnI	CCSGG	3 cut(s)	271, 469, 2155
BcoDI	GTCTC	5 cut(s)	271, 356, 711, 823, 1216
BfmI	CTRYAG	3 cut(s)	1755, 2181, 2627
BfuAI	ACCTGC	1 cut(s)	1699
BfuI	GTATCC	1 cut(s)	1835
BlnI	CCTAGG	1 cut(s)	895
BmcAI	AGTACT	1 cut(s)	2532
Bme1390I	CCNGG	8 cut(s)	271, 469, 711, 794, 1101, 1517, 1857, 2155
Bme18I	GGWCC	5 cut(s)	707, 1054, 1238, 1361, 1466
BmgBI	CACGTC	2 cut(s)	655, 2042
BmgT120I	GGNCC	5 cut(s)	707, 1054, 1238, 1361, 1466
BmiI	GGNNCC	8 cut(s)	225, 548, 708, 790, 1033, 1056, 1270, 1672
BmrFI	CCNGG	8 cut(s)	271, 469, 711, 794, 1101, 1517, 1857, 2155
BmrI	ACTGGG	2 cut(s)	1550, 1823
BmuI	ACTGGG	2 cut(s)	1550, 1823
BplI	GAGNNNNNCTC	2 cut(s)	12, 44
BpmI	CTGGAG	3 cut(s)	997, 2105, 2268
BpuEI	CTTGAG	5 cut(s)	1070, 1299, 1840, 1993, 2611
BpuMI	CCSGG	3 cut(s)	271, 469, 2155
Bsa29I	ATCGAT	1 cut(s)	2393
BsaBI	GATNNNNATC	1 cut(s)	1476
BsaHI	GRCGYC	2 cut(s)	264, 464
BsaI	GGTCTC	1 cut(s)	823
BsaJI	CCNNGG	6 cut(s)	240, 792, 895, 1116, 1899, 2154
BsaXI	ACNNNNNCTCC	4 cut(s)	1071, 1101, 1605, 1635
Bsc4I	CCNNNNNNNGG	6 cut(s)	340, 844, 1121, 1862, 2128, 2160
Bse118I	RCCGGY	1 cut(s)	868
Bse1I	ACTGG	5 cut(s)	1270, 1545, 1638, 1829, 2442
Bse3DI	GCAATG	5 cut(s)	630, 696, 1481, 1704, 2295
Bse8I	GATNNNNATC	1 cut(s)	1476
BseBI	CCWGG	5 cut(s)	711, 794, 1101, 1517, 1857
BseCI	ATCGAT	1 cut(s)	2393
BseDI	CCNNGG	6 cut(s)	240, 792, 895, 1116, 1899, 2154
BseGI	GGATG	9 cut(s)	279, 477, 853, 1126, 1159, 1240, 1315, 1516, 2511
BseJI	GATNNNNATC	1 cut(s)	1476
BseLI	CCNNNNNNNGG	6 cut(s)	340, 844, 1121, 1862, 2128, 2160
BseMI	GCAATG	5 cut(s)	630, 696, 1481, 1704, 2295
BseMII	CTCAG	5 cut(s)	1011, 1227, 1235, 1404, 2091
BseNI	ACTGG	5 cut(s)	1270, 1545, 1638, 1829, 2442
BsePI	GCGCGC	1 cut(s)	229
BseRI	GAGGAG	6 cut(s)	137, 236, 416, 472, 539, 1626
BseSI	GKGCMC	1 cut(s)	1540
Bsh1236I	CGCG	8 cut(s)	206, 229, 231, 242, 298, 398, 449, 992
Bsh1285I	CGRYCG	2 cut(s)	73, 204
BshFI	GGCC	6 cut(s)	189, 578, 1136, 1637, 1887, 2588
BshVI	ATCGAT	1 cut(s)	2393
BsiEI	CGRYCG	2 cut(s)	73, 204
BsiHKAI	GWGCWC	4 cut(s)	660, 894, 1540, 2597
BsiSI	CCGG	9 cut(s)	270, 468, 869, 1137, 1218, 1359, 2089, 2154, 2526
BslFI	GGGAC	4 cut(s)	720, 1040, 2171, 2376
BslI	CCNNNNNNNGG	6 cut(s)	340, 844, 1121, 1862, 2128, 2160
BsmAI	GTCTC	5 cut(s)	271, 356, 711, 823, 1216
BsmBI	CGTCTC	2 cut(s)	271, 356
BsmFI	GGGAC	4 cut(s)	720, 1040, 2171, 2376
BsmI	GAATGC	1 cut(s)	613
BsnI	GGCC	6 cut(s)	189, 578, 1136, 1637, 1887, 2588
Bso31I	GGTCTC	1 cut(s)	823
Bsp1286I	GDGCHC	4 cut(s)	660, 894, 1540, 2597
Bsp143I	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
Bsp68I	TCGCGA	1 cut(s)	298
BspANI	GGCC	6 cut(s)	189, 578, 1136, 1637, 1887, 2588
BspCNI	CTCAG	5 cut(s)	1010, 1226, 1236, 1403, 2092
BspDI	ATCGAT	1 cut(s)	2393
BspFNI	CGCG	8 cut(s)	206, 229, 231, 242, 298, 398, 449, 992
BspHI	TCATGA	1 cut(s)	1921
BspLI	GGNNCC	8 cut(s)	225, 548, 708, 790, 1033, 1056, 1270, 1672
BspMI	ACCTGC	1 cut(s)	1699
BspPI	GGATC	4 cut(s)	649, 763, 932, 1472
BspQI	GCTCTTC	1 cut(s)	2355
BspTNI	GGTCTC	1 cut(s)	823
BsrDI	GCAATG	5 cut(s)	630, 696, 1481, 1704, 2295
BsrFI	RCCGGY	1 cut(s)	868
BsrI	ACTGG	5 cut(s)	1270, 1545, 1638, 1829, 2442
BssAI	RCCGGY	1 cut(s)	868
BssECI	CCNNGG	6 cut(s)	240, 792, 895, 1116, 1899, 2154
BssHII	GCGCGC	1 cut(s)	229
BssMI	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
BssNI	GRCGYC	2 cut(s)	264, 464
BssSI	CACGAG	1 cut(s)	572
BssT1I	CCWWGG	1 cut(s)	895
Bst2BI	CACGAG	1 cut(s)	572
Bst2UI	CCWGG	5 cut(s)	711, 794, 1101, 1517, 1857
Bst4CI	ACNGT	6 cut(s)	140, 551, 1024, 1210, 2110, 2143
Bst6I	CTCTTC	5 cut(s)	449, 828, 1120, 2160, 2355
BstACI	GRCGYC	2 cut(s)	264, 464
BstC8I	GCNNGC	4 cut(s)	191, 231, 1344, 1974
BstDEI	CTNAG	5 cut(s)	997, 1213, 1244, 1390, 2100
BstDSI	CCRYGG	3 cut(s)	240, 1116, 1899
BstF5I	GGATG	9 cut(s)	279, 477, 853, 1126, 1159, 1240, 1315, 1516, 2511
BstFNI	CGCG	8 cut(s)	206, 229, 231, 242, 298, 398, 449, 992
BstHHI	GCGC	3 cut(s)	231, 233, 398
BstKTI	GATC	8 cut(s)	204, 644, 758, 927, 1480, 1648, 1952, 1988
BstMAI	GTCTC	5 cut(s)	271, 356, 711, 823, 1216
BstMBI	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
BstMCI	CGRYCG	2 cut(s)	73, 204
BstMWI	GCNNNNNNNGC	9 cut(s)	239, 245, 404, 695, 874, 2183, 2384, 2459, 2629
BstNI	CCWGG	5 cut(s)	711, 794, 1101, 1517, 1857
BstNSI	RCATGY	3 cut(s)	355, 599, 1976
BstSCI	CCNGG	8 cut(s)	269, 467, 709, 792, 1099, 1515, 1855, 2153
BstSFI	CTRYAG	3 cut(s)	1755, 2181, 2627
BstSLI	GKGCMC	1 cut(s)	1540
BstUI	CGCG	8 cut(s)	206, 229, 231, 242, 298, 398, 449, 992
BstX2I	RGATCY	1 cut(s)	1477
BstXI	CCANNNNNNTGG	1 cut(s)	1813
BstYI	RGATCY	1 cut(s)	1477
Bsu15I	ATCGAT	1 cut(s)	2393
BsuI	GTATCC	1 cut(s)	1835
BsuRI	GGCC	6 cut(s)	189, 578, 1136, 1637, 1887, 2588
BsuTUI	ATCGAT	1 cut(s)	2393
BtgI	CCRYGG	3 cut(s)	240, 1116, 1899
BtgZI	GCGATG	1 cut(s)	113
BtrI	CACGTC	2 cut(s)	655, 2042
BtsCI	GGATG	9 cut(s)	279, 477, 853, 1126, 1159, 1240, 1315, 1516, 2511
BtsI	GCAGTG	1 cut(s)	2618
BtsIMutI	CAGTG	6 cut(s)	817, 959, 1020, 1538, 2435, 2618
BtuMI	TCGCGA	1 cut(s)	298
BveI	ACCTGC	1 cut(s)	1699
Cac8I	GCNNGC	4 cut(s)	191, 231, 1344, 1974
CciI	TCATGA	1 cut(s)	1921
CfoI	GCGC	3 cut(s)	231, 233, 398
Cfr10I	RCCGGY	1 cut(s)	868
Cfr13I	GGNCC	5 cut(s)	707, 1054, 1238, 1361, 1466
Cfr42I	CCGCGG	1 cut(s)	243
ClaI	ATCGAT	1 cut(s)	2393
CseI	GACGC	3 cut(s)	237, 453, 981
Csp6I	GTAC	4 cut(s)	1025, 1414, 1764, 2531
CviQI	GTAC	4 cut(s)	1025, 1414, 1764, 2531
DdeI	CTNAG	5 cut(s)	997, 1213, 1244, 1390, 2100
DpnI	GATC	8 cut(s)	203, 643, 757, 926, 1479, 1647, 1951, 1987
DpnII	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
DraIII	CACNNNGTG	1 cut(s)	340
EaeI	YGGCCR	2 cut(s)	1134, 1635
Eam1104I	CTCTTC	5 cut(s)	449, 828, 1120, 2160, 2355
EarI	CTCTTC	5 cut(s)	449, 828, 1120, 2160, 2355
EciI	GGCGGA	4 cut(s)	140, 401, 425, 497
Ecl136II	GAGCTC	1 cut(s)	2595
Eco130I	CCWWGG	1 cut(s)	895
Eco24I	GRGCYC	1 cut(s)	2597
Eco31I	GGTCTC	1 cut(s)	823
Eco47I	GGWCC	5 cut(s)	707, 1054, 1238, 1361, 1466
Eco53kI	GAGCTC	1 cut(s)	2595
Eco57I	CTGAAG	2 cut(s)	401, 2336
EcoICRI	GAGCTC	1 cut(s)	2595
EcoO109I	RGGNCCY	1 cut(s)	1054
EcoRI	GAATTC	2 cut(s)	624, 769
EcoRII	CCWGG	5 cut(s)	709, 792, 1099, 1515, 1855
EcoT14I	CCWWGG	1 cut(s)	895
EcoT38I	GRGCYC	1 cut(s)	2597
ErhI	CCWWGG	1 cut(s)	895
Esp3I	CGTCTC	2 cut(s)	271, 356
FalI	AAGNNNNNCTT	2 cut(s)	1587, 1619
FaqI	GGGAC	4 cut(s)	720, 1040, 2171, 2376
FauI	CCCGC	1 cut(s)	415
FblI	GTMKAC	1 cut(s)	69
FokI	GGATG	9 cut(s)	286, 484, 860, 1133, 1166, 1247, 1322, 1523, 2498
FriOI	GRGCYC	1 cut(s)	2597
GlaI	GCGC	3 cut(s)	230, 232, 397
GsuI	CTGGAG	3 cut(s)	997, 2105, 2268
HaeIII	GGCC	6 cut(s)	189, 578, 1136, 1637, 1887, 2588
HapII	CCGG	9 cut(s)	270, 468, 869, 1137, 1218, 1359, 2089, 2154, 2526
HgaI	GACGC	3 cut(s)	237, 453, 981
HhaI	GCGC	3 cut(s)	231, 233, 398
Hin1I	GRCGYC	2 cut(s)	264, 464
Hin6I	GCGC	3 cut(s)	229, 231, 396
HinP1I	GCGC	3 cut(s)	229, 231, 396
HincII	GTYRAC	4 cut(s)	70, 349, 357, 432
HindII	GTYRAC	4 cut(s)	70, 349, 357, 432
HpaII	CCGG	9 cut(s)	270, 468, 869, 1137, 1218, 1359, 2089, 2154, 2526
HphI	GGTGA	3 cut(s)	128, 523, 2309
Hpy166II	GTNNAC	9 cut(s)	54, 70, 334, 349, 357, 432, 593, 1538, 2106
Hpy188III	TCNNGA	9 cut(s)	218, 297, 538, 908, 978, 1439, 1922, 1933, 2247
Hpy8I	GTNNAC	9 cut(s)	54, 70, 334, 349, 357, 432, 593, 1538, 2106
Hpy99I	CGWCG	3 cut(s)	120, 253, 266
HpyCH4III	ACNGT	6 cut(s)	140, 551, 1024, 1210, 2110, 2143
HpyCH4IV	ACGT	6 cut(s)	264, 654, 901, 2041, 2337, 2502
HpyF10VI	GCNNNNNNNGC	9 cut(s)	239, 245, 404, 695, 874, 2183, 2384, 2459, 2629
HpyF3I	CTNAG	5 cut(s)	997, 1213, 1244, 1390, 2100
HpySE526I	ACGT	6 cut(s)	264, 654, 901, 2041, 2337, 2502
Hsp92I	GRCGYC	2 cut(s)	264, 464
HspAI	GCGC	3 cut(s)	229, 231, 396
KspI	CCGCGG	1 cut(s)	243
Kzo9I	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
LguI	GCTCTTC	1 cut(s)	2355
LmnI	GCTCC	8 cut(s)	223, 663, 897, 1037, 1274, 1586, 1613, 2249
MaeII	ACGT	6 cut(s)	264, 654, 901, 2041, 2337, 2502
MaeIII	GTNAC	5 cut(s)	134, 957, 1831, 2110, 2608
MalI	GATC	8 cut(s)	203, 643, 757, 926, 1479, 1647, 1951, 1987
MboI	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
MfeI	CAATTG	2 cut(s)	1779, 2025
MflI	RGATCY	1 cut(s)	1477
MhlI	GDGCHC	4 cut(s)	660, 894, 1540, 2597
MlsI	TGGCCA	1 cut(s)	1637
MluNI	TGGCCA	1 cut(s)	1637
MlyI	GAGTC	9 cut(s)	309, 339, 562, 1166, 1378, 1593, 1736, 2112, 2224
MmeI	TCCRAC	3 cut(s)	2017, 2103, 2176
Mox20I	TGGCCA	1 cut(s)	1637
MroXI	GAANNNNTTC	3 cut(s)	1179, 1737, 1917
MscI	TGGCCA	1 cut(s)	1637
MseI	TTAA	6 cut(s)	375, 828, 1191, 1796, 1847, 2265
Msp20I	TGGCCA	1 cut(s)	1637
MspA1I	CMGCKG	1 cut(s)	242
MspI	CCGG	9 cut(s)	270, 468, 869, 1137, 1218, 1359, 2089, 2154, 2526
MspR9I	CCNGG	8 cut(s)	271, 469, 711, 794, 1101, 1517, 1857, 2155
MunI	CAATTG	2 cut(s)	1779, 2025
Mva1269I	GAATGC	1 cut(s)	613
MvaI	CCWGG	5 cut(s)	711, 794, 1101, 1517, 1857
MvnI	CGCG	8 cut(s)	206, 229, 231, 242, 298, 398, 449, 992
MwoI	GCNNNNNNNGC	9 cut(s)	239, 245, 404, 695, 874, 2183, 2384, 2459, 2629
NciI	CCSGG	3 cut(s)	271, 469, 2155
NdeII	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
NlaIV	GGNNCC	8 cut(s)	225, 548, 708, 790, 1033, 1056, 1270, 1672
NmeAIII	GCCGAG	1 cut(s)	1008
NmuCI	GTSAC	3 cut(s)	134, 957, 1831
NruI	TCGCGA	1 cut(s)	298
NspI	RCATGY	3 cut(s)	355, 599, 1976
PaeI	GCATGC	1 cut(s)	1976
PagI	TCATGA	1 cut(s)	1921
PauI	GCGCGC	1 cut(s)	229
PciI	ACATGT	1 cut(s)	351
PciSI	GCTCTTC	1 cut(s)	2355
PcsI	WCGNNNNNNNCGW	2 cut(s)	38, 136
PctI	GAATGC	1 cut(s)	613
PdmI	GAANNNNTTC	3 cut(s)	1179, 1737, 1917
PfeI	GAWTC	6 cut(s)	196, 444, 1007, 1082, 1175, 1349
PflFI	GACNNNGTC	1 cut(s)	329
PflMI	CCANNNNNTGG	2 cut(s)	340, 1862
PfoI	TCCNGGA	2 cut(s)	269, 467
Ple19I	CGATCG	1 cut(s)	204
PleI	GAGTC	9 cut(s)	308, 338, 562, 1165, 1378, 1593, 1735, 2112, 2224
PpsI	GAGTC	9 cut(s)	308, 338, 562, 1165, 1378, 1593, 1735, 2112, 2224
PpuMI	RGGWCCY	1 cut(s)	1054
PscI	ACATGT	1 cut(s)	351
Psp124BI	GAGCTC	1 cut(s)	2597
Psp5II	RGGWCCY	1 cut(s)	1054
Psp6I	CCWGG	5 cut(s)	709, 792, 1099, 1515, 1855
PspGI	CCWGG	5 cut(s)	709, 792, 1099, 1515, 1855
PspN4I	GGNNCC	8 cut(s)	225, 548, 708, 790, 1033, 1056, 1270, 1672
PspPI	GGNCC	5 cut(s)	707, 1054, 1238, 1361, 1466
PspPPI	RGGWCCY	1 cut(s)	1054
PsuI	RGATCY	1 cut(s)	1477
PsyI	GACNNNGTC	1 cut(s)	329
PteI	GCGCGC	1 cut(s)	229
PvuI	CGATCG	1 cut(s)	204
RruI	TCGCGA	1 cut(s)	298
RsaI	GTAC	4 cut(s)	1026, 1415, 1765, 2532
RsaNI	GTAC	4 cut(s)	1025, 1414, 1764, 2531
SacI	GAGCTC	1 cut(s)	2597
SacII	CCGCGG	1 cut(s)	243
SalI	GTCGAC	1 cut(s)	68
SapI	GCTCTTC	1 cut(s)	2355
SaqAI	TTAA	6 cut(s)	375, 828, 1191, 1796, 1847, 2265
Sau3AI	GATC	8 cut(s)	201, 641, 755, 924, 1477, 1645, 1949, 1985
Sau96I	GGNCC	5 cut(s)	707, 1054, 1238, 1361, 1466
ScaI	AGTACT	1 cut(s)	2532
SchI	GAGTC	9 cut(s)	309, 339, 562, 1166, 1378, 1593, 1736, 2112, 2224
ScrFI	CCNGG	8 cut(s)	271, 469, 711, 794, 1101, 1517, 1857, 2155
SduI	GDGCHC	4 cut(s)	660, 894, 1540, 2597
SfcI	CTRYAG	3 cut(s)	1755, 2181, 2627
Sfr303I	CCGCGG	1 cut(s)	243
SgrBI	CCGCGG	1 cut(s)	243
SinI	GGWCC	5 cut(s)	707, 1054, 1238, 1361, 1466
SmlI	CTYRAG	5 cut(s)	1049, 1314, 1819, 2008, 2590
SmoI	CTYRAG	5 cut(s)	1049, 1314, 1819, 2008, 2590
SphI	GCATGC	1 cut(s)	1976
SstI	GAGCTC	1 cut(s)	2597
StyD4I	CCNGG	8 cut(s)	269, 467, 709, 792, 1099, 1515, 1855, 2153
StyI	CCWWGG	1 cut(s)	895
TaaI	ACNGT	6 cut(s)	140, 551, 1024, 1210, 2110, 2143
TaiI	ACGT	6 cut(s)	267, 657, 904, 2044, 2340, 2505
TaqI	TCGA	5 cut(s)	69, 261, 1984, 2393, 2597
TaqII	GACCGA	3 cut(s)	342, 374, 1962
TatI	WGTACW	3 cut(s)	1024, 1763, 2530
TfiI	GAWTC	6 cut(s)	196, 444, 1007, 1082, 1175, 1349
Tru1I	TTAA	6 cut(s)	375, 828, 1191, 1796, 1847, 2265
Tru9I	TTAA	6 cut(s)	375, 828, 1191, 1796, 1847, 2265
TscAI	CASTG	6 cut(s)	817, 966, 1027, 1545, 2442, 2625
TseFI	GTSAC	3 cut(s)	134, 957, 1831
Tsp45I	GTSAC	3 cut(s)	134, 957, 1831
TspDTI	ATGAA	7 cut(s)	17, 782, 959, 963, 1338, 1910, 2624
TspGWI	ACGGA	2 cut(s)	449, 1694
TspRI	CASTG	6 cut(s)	817, 966, 1027, 1545, 2442, 2625
Tth111I	GACNNNGTC	1 cut(s)	329
Van91I	CCANNNNNTGG	2 cut(s)	340, 1862
VneI	GTGCAC	1 cut(s)	1536
VpaK11BI	GGWCC	5 cut(s)	707, 1054, 1238, 1361, 1466
XapI	RAATTY	6 cut(s)	624, 769, 1734, 1956, 2210, 2476
XceI	RCATGY	3 cut(s)	355, 599, 1976
XmaJI	CCTAGG	1 cut(s)	895
XmiI	GTMKAC	1 cut(s)	69
XmnI	GAANNNNTTC	3 cut(s)	1179, 1737, 1917
ZraI	GACGTC	1 cut(s)	265
ZrmI	AGTACT	1 cut(s)	2532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.