Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0009089

Physical Location & Seq

Genomic Coordinates Forward (+)

67982865 .. 67990261

Gene Structure 7397 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG524700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGATGAATACTATTCTGACCAGAGCCTCTCTCTCGCCGGACCCGAAGCTCCTGTTCAGCTACACCACCAACTCCAATGTCGACCGCTTCGCCTTCAACTTCAAGCCTCACCTCCCGATACGCCGTCGTTTCCCTCATCGCCGTCATCTCCGGCAGCAGTCGAACCCTAATTTCACTCTCTCCAGCTGCCTGCAGGCCGCCGCGGCCCCAAATCATATCGCCACGGCGGCGGCGCCGATTCAGGAGGAGGCGTCGGTGCTGCTCGACGTCTCCGGGATGATGTGCGGCGGCTGTGTTTCGCGAGTCAAGTCGGTGCTGTCTGCGGACGACCGAGTCCACTCGGTGGCGGTCAACATGTTGACCGAGACGGCAGCGATTAAGCTGAGGCCGGAGGTTGAAGGTGGAGCGCAGGCCGTGGCGGAGAGCTTGGCCGGAAGGTTGACGGAGTGCGGATTCGCGGCGAGGAGGAGGGCTTCCGGGATGGGAGTGACGGAGAGTGTGAGGAAGTGGAAGGAGATGGTGAAGAAGAAGGAGGAGATGCTGGTGAAGAGTAGGAACCGAGTCATTTTGGCTTGGACTCTGGTGGCCTTGTGCTGTGGATCACACGCTTCCCACATTTTGCATTCTCTGGGAATTCACATTGCTCATGGATCGTATATGGATGTGCTCCATAATTCGTATGTGAAGGGTGGTTTGGCTATGGCAGCTTTGTTGGGACCGGGACGAGGAGCAAGGCAAGGACTCCTTGTGAGAGGAGCAGATGTATTGGAACGCTTGGCCAGTATAGATCATATTGCTTTAGACAAGACAGGAACCCTCACTGAAGGAAAACCTGCTGTTTCTAGCATTGCTTCTTTCAAGTATAAAGACTCAGAAATTCTTCAAATAGCTGCTGCTGTAGAGAGTACAGCATCACATCCAATTGCTGATGCTATCTTAAACAAAGCCAAATCATTGGAGTTGAGTATCCCAGTCACAAAAAGGCAATTAACAGAACCAGGTTTTGGAACATTAGCAGAAGTAGATGGCCTTTTGGTTGCAGTGGGTTCTTTGGAATGGGTTCATGAGCGTTTCCAGAGAAGAACAGATCGATCTGACATTTCAAACCTAGAGCATGCCGTGCATCAATCATCAGAAGGCATAACACCCTCAAATTATTCAAAAACAATTGTATACGTTGGACGTGAAGGGGAAGGTATCATCGGTGCTATTGCAATATCCGACAGCCTGCGGGAAGATGCTGAGTTTACTGTAACTAGACTCCAGCAGAAGGGTATCAGAACTGTCCTGTTTTCTGGGGACAGGGAAGAGGCAGTTGCAACTATAGCAAAATCAGTTGGAATAGAAAAGGAATTTATCAAGTCATCGTTGACTCCACAGGGAAAATCTGGAGCTATTTCTAGTCTTAAAGCTGCAGGGCATCATGTTGCAATGGTAACCATCTCCAATTTGTTCTGTAAATTTTGCTTTTTCATATGTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0035434 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

494

Amino Acids

53.39

Weight (kDa)

9.21

Isoelectric Point (pI)

41.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	88 - 132	2.3e-09	Heavy-metal-associated domain
Hydrolase	PF00702	262 - 479	2.5e-17	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21930 AT5G21930 AT5G21930 AT5G21930
fragaria_vesca	FvH4_2g30930 FvH4_2g30930 FvH4_2g30930
malus_domestica	MD15G1049400.v1.1
prunus_persica	Prupe.1G403700_v2.0.a1 Prupe.1G403700_v2.0.a1
pyrus_communis	pycom15g04820
rosa_chinensis	RchiOBHm_Chr6g0311921
rosa_multiflora	Rmu_ssc0000057.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157220
rosa_rugosa	Rorug06G0391300 Rorug06G0391400 Rorug06G0391400
rosa_samantha	Rh6AG507800 Rh6BG519000 Rh6CG524700 Rh6DG510300
rosa_wichuraiana	Rw6G044200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	332
AatII	GACGTC	1 cut(s)	270
Acc36I	ACCTGC	1 cut(s)	841
AccB1I	GGYRCC	1 cut(s)	232
AccB7I	CCANNNNNTGG	2 cut(s)	343, 1004
AccI	GTMKAC	2 cut(s)	81, 1173
AccII	CGCG	3 cut(s)	203, 301, 458
AclWI	GGATC	2 cut(s)	607, 658
AcoI	YGGCCR	2 cut(s)	429, 777
AcsI	RAATTY	4 cut(s)	633, 876, 1352, 1460
AcuI	CTGAAG	1 cut(s)	843
AcyI	GRCGYC	3 cut(s)	233, 251, 267
AdeI	CACNNNGTG	1 cut(s)	343
AfaI	GTAC	1 cut(s)	907
AfiI	CCNNNNNNNGG	4 cut(s)	343, 725, 1004, 1270
AflIII	ACRYGT	1 cut(s)	354
AgsI	TTSAA	7 cut(s)	97, 103, 398, 859, 884, 1104, 1161
AjiI	CACGTC	1 cut(s)	1184
AjnI	CCWGG	1 cut(s)	997
AjuI	GAANNNNNNNTTGG	4 cut(s)	677, 709, 987, 1019
AluBI	AGCT	9 cut(s)	49, 60, 186, 382, 426, 707, 890, 1394, 1412
AluI	AGCT	9 cut(s)	49, 60, 186, 382, 426, 707, 890, 1394, 1412
Alw21I	GWGCWC	1 cut(s)	669
Alw26I	GTCTC	2 cut(s)	274, 359
AlwI	GGATC	2 cut(s)	607, 658
AoxI	GGCC	8 cut(s)	195, 204, 386, 411, 429, 585, 777, 1027
ApeKI	GCWGC	8 cut(s)	154, 186, 259, 371, 704, 890, 893, 1412
ApoI	RAATTY	4 cut(s)	633, 876, 1352, 1460
Asp700I	GAANNNNTTC	2 cut(s)	879, 1059
AspLEI	GCGC	2 cut(s)	235, 409
AspS9I	GGNCC	3 cut(s)	40, 205, 716
AsuC2I	CCSGG	3 cut(s)	274, 478, 720
AsuHPI	GGTGA	3 cut(s)	101, 532, 556
AvaII	GGWCC	2 cut(s)	40, 716
BalI	TGGCCA	1 cut(s)	779
BanI	GGYRCC	1 cut(s)	232
Bbv12I	GWGCWC	1 cut(s)	669
BbvCI	CCTCAGC	1 cut(s)	383
BbvI	GCAGC	8 cut(s)	166, 173, 246, 383, 716, 877, 880, 1399
BccI	CCATC	4 cut(s)	475, 511, 1019, 1448
BceAI	ACGGC	6 cut(s)	108, 126, 240, 384, 398, 1103
BciT130I	CCWGG	1 cut(s)	999
BciVI	GTATCC	1 cut(s)	977
BcnI	CCSGG	3 cut(s)	274, 478, 720
BcoDI	GTCTC	2 cut(s)	274, 359
BfaI	CTAG	4 cut(s)	843, 1109, 1257, 1401
BfmI	CTRYAG	4 cut(s)	191, 897, 1323, 1413
BfoI	RGCGCY	1 cut(s)	236
BfuAI	ACCTGC	1 cut(s)	841
BfuI	GTATCC	1 cut(s)	977
BglI	GCCNNNNNGGC	2 cut(s)	203, 227
Bme1390I	CCNGG	4 cut(s)	274, 478, 720, 999
Bme18I	GGWCC	2 cut(s)	40, 716
BmgBI	CACGTC	1 cut(s)	1184
BmgT120I	GGNCC	3 cut(s)	40, 205, 716
BmiI	GGNNCC	6 cut(s)	42, 207, 234, 557, 717, 814
BmrFI	CCNGG	4 cut(s)	274, 478, 720, 999
BmrI	ACTGGG	1 cut(s)	965
BmsI	GCATC	6 cut(s)	528, 919, 920, 1132, 1228, 1429
BmuI	ACTGGG	1 cut(s)	965
BplI	GAGNNNNNCTC	2 cut(s)	15, 47
BpmI	CTGGAG	3 cut(s)	166, 1247, 1410
Bpu10I	CCTNAGC	1 cut(s)	383
BpuMI	CCSGG	3 cut(s)	274, 478, 720
Bsa29I	ATCGAT	1 cut(s)	1090
BsaHI	GRCGYC	3 cut(s)	233, 251, 267
BsaJI	CCNNGG	3 cut(s)	201, 222, 414
BsaXI	ACNNNNNCTCC	4 cut(s)	236, 266, 747, 777
Bsc4I	CCNNNNNNNGG	4 cut(s)	343, 725, 1004, 1270
Bse1I	ACTGG	2 cut(s)	780, 971
Bse3DI	GCAATG	3 cut(s)	639, 846, 1437
BseBI	CCWGG	1 cut(s)	999
BseCI	ATCGAT	1 cut(s)	1090
BseDI	CCNNGG	3 cut(s)	201, 222, 414
BseGI	GGATG	4 cut(s)	282, 486, 667, 916
BseLI	CCNNNNNNNGG	4 cut(s)	343, 725, 1004, 1270
BseMI	GCAATG	3 cut(s)	639, 846, 1437
BseMII	CTCAG	3 cut(s)	374, 885, 1233
BseNI	ACTGG	2 cut(s)	780, 971
BseRI	GAGGAG	6 cut(s)	260, 478, 481, 548, 741, 768
BseXI	GCAGC	8 cut(s)	166, 173, 246, 383, 716, 877, 880, 1399
Bsh1236I	CGCG	3 cut(s)	203, 301, 458
Bsh1285I	CGRYCG	2 cut(s)	85, 331
BshFI	GGCC	8 cut(s)	197, 206, 388, 413, 431, 587, 779, 1029
BshNI	GGYRCC	1 cut(s)	232
BshVI	ATCGAT	1 cut(s)	1090
BsiEI	CGRYCG	2 cut(s)	85, 331
BsiHKAI	GWGCWC	1 cut(s)	669
BsiSI	CCGG	7 cut(s)	38, 151, 273, 389, 432, 477, 719
BslFI	GGGAC	3 cut(s)	729, 735, 1313
BslI	CCNNNNNNNGG	4 cut(s)	343, 725, 1004, 1270
BsmAI	GTCTC	2 cut(s)	274, 359
BsmBI	CGTCTC	2 cut(s)	274, 359
BsmFI	GGGAC	3 cut(s)	729, 735, 1313
BsmI	GAATGC	1 cut(s)	622
BsnI	GGCC	8 cut(s)	197, 206, 388, 413, 431, 587, 779, 1029
Bsp1286I	GDGCHC	1 cut(s)	669
Bsp143I	GATC	5 cut(s)	599, 650, 787, 1087, 1091
Bsp68I	TCGCGA	1 cut(s)	301
BspANI	GGCC	8 cut(s)	197, 206, 388, 413, 431, 587, 779, 1029
BspCNI	CTCAG	3 cut(s)	375, 884, 1234
BspDI	ATCGAT	1 cut(s)	1090
BspFNI	CGCG	3 cut(s)	203, 301, 458
BspHI	TCATGA	1 cut(s)	1063
BspLI	GGNNCC	6 cut(s)	42, 207, 234, 557, 717, 814
BspMAI	CTGCAG	2 cut(s)	195, 1417
BspMI	ACCTGC	1 cut(s)	841
BspPI	GGATC	2 cut(s)	607, 658
BspT107I	GGYRCC	1 cut(s)	232
BsrDI	GCAATG	3 cut(s)	639, 846, 1437
BsrI	ACTGG	2 cut(s)	780, 971
BssECI	CCNNGG	3 cut(s)	201, 222, 414
BssMI	GATC	5 cut(s)	599, 650, 787, 1087, 1091
BssNAI	GTATAC	1 cut(s)	1174
BssNI	GRCGYC	3 cut(s)	233, 251, 267
Bst1107I	GTATAC	1 cut(s)	1174
Bst2UI	CCWGG	1 cut(s)	999
Bst4CI	ACNGT	2 cut(s)	1252, 1285
Bst6I	CTCTTC	2 cut(s)	542, 1302
BstACI	GRCGYC	3 cut(s)	233, 251, 267
BstAPI	GCANNNNNTGC	1 cut(s)	1120
BstC8I	GCNNGC	5 cut(s)	191, 195, 411, 1116, 1229
BstDEI	CTNAG	3 cut(s)	383, 871, 1242
BstDSI	CCRYGG	3 cut(s)	201, 222, 414
BstEII	GGTNACC	1 cut(s)	1435
BstF5I	GGATG	4 cut(s)	282, 486, 667, 916
BstFNI	CGCG	3 cut(s)	203, 301, 458
BstH2I	RGCGCY	1 cut(s)	236
BstHHI	GCGC	2 cut(s)	235, 409
BstKTI	GATC	5 cut(s)	602, 653, 790, 1090, 1094
BstMAI	GTCTC	2 cut(s)	274, 359
BstMBI	GATC	5 cut(s)	599, 650, 787, 1087, 1091
BstMCI	CGRYCG	2 cut(s)	85, 331
BstMWI	GCNNNNNNNGC	5 cut(s)	203, 227, 704, 1120, 1325
BstNI	CCWGG	1 cut(s)	999
BstNSI	RCATGY	2 cut(s)	358, 1118
BstPI	GGTNACC	1 cut(s)	1435
BstSCI	CCNGG	4 cut(s)	272, 476, 718, 997
BstSFI	CTRYAG	4 cut(s)	191, 897, 1323, 1413
BstUI	CGCG	3 cut(s)	203, 301, 458
BstV1I	GCAGC	8 cut(s)	166, 173, 246, 383, 716, 877, 880, 1399
BstXI	CCANNNNNNTGG	1 cut(s)	955
BstZ17I	GTATAC	1 cut(s)	1174
Bsu15I	ATCGAT	1 cut(s)	1090
BsuI	GTATCC	1 cut(s)	977
BsuRI	GGCC	8 cut(s)	197, 206, 388, 413, 431, 587, 779, 1029
BsuTUI	ATCGAT	1 cut(s)	1090
BtgI	CCRYGG	3 cut(s)	201, 222, 414
BtgZI	GCGATG	1 cut(s)	122
BtrI	CACGTC	1 cut(s)	1184
BtsCI	GGATG	4 cut(s)	282, 486, 667, 916
BtsI	GCAGTG	1 cut(s)	1047
BtsIMutI	CAGTG	2 cut(s)	819, 1047
BtuMI	TCGCGA	1 cut(s)	301
BveI	ACCTGC	1 cut(s)	841
Cac8I	GCNNGC	5 cut(s)	191, 195, 411, 1116, 1229
CciI	TCATGA	1 cut(s)	1063
CfoI	GCGC	2 cut(s)	235, 409
Cfr13I	GGNCC	3 cut(s)	40, 205, 716
Cfr42I	CCGCGG	1 cut(s)	204
ClaI	ATCGAT	1 cut(s)	1090
CseI	GACGC	1 cut(s)	240
CsiI	ACCWGGT	1 cut(s)	997
Csp6I	GTAC	1 cut(s)	906
CviAII	CATG	5 cut(s)	355, 647, 1064, 1115, 1424
CviQI	GTAC	1 cut(s)	906
DdeI	CTNAG	3 cut(s)	383, 871, 1242
DinI	GGCGCC	1 cut(s)	234
DpnI	GATC	5 cut(s)	601, 652, 789, 1089, 1093
DpnII	GATC	5 cut(s)	599, 650, 787, 1087, 1091
DraIII	CACNNNGTG	1 cut(s)	343
DrdI	GACNNNNNNGTC	1 cut(s)	332
DseDI	GACNNNNNNGTC	1 cut(s)	332
EaeI	YGGCCR	2 cut(s)	429, 777
Eam1104I	CTCTTC	2 cut(s)	542, 1302
EarI	CTCTTC	2 cut(s)	542, 1302
EciI	GGCGGA	1 cut(s)	434
Eco47I	GGWCC	2 cut(s)	40, 716
Eco57I	CTGAAG	1 cut(s)	843
Eco91I	GGTNACC	1 cut(s)	1435
EcoO65I	GGTNACC	1 cut(s)	1435
EcoRI	GAATTC	1 cut(s)	633
EcoRII	CCWGG	1 cut(s)	997
EgeI	GGCGCC	1 cut(s)	234
EheI	GGCGCC	1 cut(s)	234
Esp3I	CGTCTC	2 cut(s)	274, 359
FaeI	CATG	5 cut(s)	358, 650, 1067, 1118, 1427
FalI	AAGNNNNNCTT	2 cut(s)	729, 761
FaqI	GGGAC	3 cut(s)	729, 735, 1313
FatI	CATG	5 cut(s)	354, 646, 1063, 1114, 1423
FauI	CCCGC	1 cut(s)	1224
FauNDI	CATATG	1 cut(s)	1475
FblI	GTMKAC	2 cut(s)	81, 1173
FokI	GGATG	4 cut(s)	289, 493, 674, 903
FspBI	CTAG	4 cut(s)	843, 1109, 1257, 1401
GlaI	GCGC	2 cut(s)	234, 408
GsuI	CTGGAG	3 cut(s)	166, 1247, 1410
HaeII	RGCGCY	1 cut(s)	236
HaeIII	GGCC	8 cut(s)	197, 206, 388, 413, 431, 587, 779, 1029
HapII	CCGG	7 cut(s)	38, 151, 273, 389, 432, 477, 719
HgaI	GACGC	1 cut(s)	240
HhaI	GCGC	2 cut(s)	235, 409
Hin1I	GRCGYC	3 cut(s)	233, 251, 267
Hin1II	CATG	5 cut(s)	358, 650, 1067, 1118, 1427
Hin6I	GCGC	2 cut(s)	233, 407
HinP1I	GCGC	2 cut(s)	233, 407
HincII	GTYRAC	5 cut(s)	82, 352, 360, 441, 1371
HindII	GTYRAC	5 cut(s)	82, 352, 360, 441, 1371
HpaII	CCGG	7 cut(s)	38, 151, 273, 389, 432, 477, 719
HphI	GGTGA	3 cut(s)	101, 532, 556
Hpy166II	GTNNAC	8 cut(s)	82, 337, 352, 360, 441, 1174, 1248, 1371
Hpy188I	TCNGA	6 cut(s)	18, 874, 1096, 1135, 1222, 1280
Hpy188III	TCNNGA	6 cut(s)	115, 242, 300, 1064, 1075, 1389
Hpy8I	GTNNAC	8 cut(s)	82, 337, 352, 360, 441, 1174, 1248, 1371
Hpy99I	CGWCG	3 cut(s)	129, 256, 269
HpyCH4III	ACNGT	2 cut(s)	1252, 1285
HpyCH4IV	ACGT	3 cut(s)	267, 1176, 1183
HpyCH4V	TGCA	8 cut(s)	193, 622, 1040, 1123, 1214, 1319, 1415, 1430
HpyF10VI	GCNNNNNNNGC	5 cut(s)	203, 227, 704, 1120, 1325
HpyF3I	CTNAG	3 cut(s)	383, 871, 1242
HpySE526I	ACGT	3 cut(s)	267, 1176, 1183
Hsp92I	GRCGYC	3 cut(s)	233, 251, 267
Hsp92II	CATG	5 cut(s)	358, 650, 1067, 1118, 1427
HspAI	GCGC	2 cut(s)	233, 407
KasI	GGCGCC	1 cut(s)	232
KspI	CCGCGG	1 cut(s)	204
Kzo9I	GATC	5 cut(s)	599, 650, 787, 1087, 1091
LmnI	GCTCC	6 cut(s)	54, 404, 672, 728, 755, 1391
Lsp1109I	GCAGC	8 cut(s)	166, 173, 246, 383, 716, 877, 880, 1399
LweI	GCATC	6 cut(s)	528, 919, 920, 1132, 1228, 1429
MabI	ACCWGGT	1 cut(s)	997
MaeI	CTAG	4 cut(s)	843, 1109, 1257, 1401
MaeII	ACGT	3 cut(s)	267, 1176, 1183
MaeIII	GTNAC	4 cut(s)	487, 973, 1252, 1435
MalI	GATC	5 cut(s)	601, 652, 789, 1089, 1093
MboI	GATC	5 cut(s)	599, 650, 787, 1087, 1091
MboII	GAAGA	7 cut(s)	535, 538, 559, 872, 1092, 1247, 1319
MfeI	CAATTG	2 cut(s)	921, 1167
MhlI	GDGCHC	1 cut(s)	669
MlsI	TGGCCA	1 cut(s)	779
MluNI	TGGCCA	1 cut(s)	779
Mly113I	GGCGCC	1 cut(s)	233
MlyI	GAGTC	8 cut(s)	312, 342, 570, 571, 735, 863, 1254, 1366
MmeI	TCCRAC	3 cut(s)	1159, 1245, 1318
Mox20I	TGGCCA	1 cut(s)	779
MroXI	GAANNNNTTC	2 cut(s)	879, 1059
MscI	TGGCCA	1 cut(s)	779
MseI	TTAA	4 cut(s)	378, 938, 989, 1407
Msp20I	TGGCCA	1 cut(s)	779
MspA1I	CMGCKG	2 cut(s)	186, 203
MspI	CCGG	7 cut(s)	38, 151, 273, 389, 432, 477, 719
MspR9I	CCNGG	4 cut(s)	274, 478, 720, 999
MunI	CAATTG	2 cut(s)	921, 1167
Mva1269I	GAATGC	1 cut(s)	622
MvaI	CCWGG	1 cut(s)	999
MvnI	CGCG	3 cut(s)	203, 301, 458
MwoI	GCNNNNNNNGC	5 cut(s)	203, 227, 704, 1120, 1325
NarI	GGCGCC	1 cut(s)	233
NciI	CCSGG	3 cut(s)	274, 478, 720
NdeI	CATATG	1 cut(s)	1475
NdeII	GATC	5 cut(s)	599, 650, 787, 1087, 1091
NlaIII	CATG	5 cut(s)	358, 650, 1067, 1118, 1427
NlaIV	GGNNCC	6 cut(s)	42, 207, 234, 557, 717, 814
NmuCI	GTSAC	2 cut(s)	487, 973
NruI	TCGCGA	1 cut(s)	301
NspI	RCATGY	2 cut(s)	358, 1118
PaeI	GCATGC	1 cut(s)	1118
PagI	TCATGA	1 cut(s)	1063
PciI	ACATGT	1 cut(s)	354
PcsI	WCGNNNNNNNCGW	1 cut(s)	41
PctI	GAATGC	1 cut(s)	622
PdmI	GAANNNNTTC	2 cut(s)	879, 1059
PfeI	GAWTC	2 cut(s)	238, 453
PflFI	GACNNNGTC	1 cut(s)	332
PflMI	CCANNNNNTGG	2 cut(s)	343, 1004
PfoI	TCCNGGA	2 cut(s)	272, 476
PleI	GAGTC	8 cut(s)	311, 341, 569, 571, 735, 863, 1254, 1366
PluTI	GGCGCC	1 cut(s)	236
PpsI	GAGTC	8 cut(s)	311, 341, 569, 571, 735, 863, 1254, 1366
PscI	ACATGT	1 cut(s)	354
Psp6I	CCWGG	1 cut(s)	997
PspEI	GGTNACC	1 cut(s)	1435
PspGI	CCWGG	1 cut(s)	997
PspN4I	GGNNCC	6 cut(s)	42, 207, 234, 557, 717, 814
PspPI	GGNCC	3 cut(s)	40, 205, 716
PstI	CTGCAG	2 cut(s)	195, 1417
PsyI	GACNNNGTC	1 cut(s)	332
PvuII	CAGCTG	1 cut(s)	186
RruI	TCGCGA	1 cut(s)	301
RsaI	GTAC	1 cut(s)	907
RsaNI	GTAC	1 cut(s)	906
SacII	CCGCGG	1 cut(s)	204
SalI	GTCGAC	1 cut(s)	80
SaqAI	TTAA	4 cut(s)	378, 938, 989, 1407
Sau3AI	GATC	5 cut(s)	599, 650, 787, 1087, 1091
Sau96I	GGNCC	3 cut(s)	40, 205, 716
SbfI	CCTGCAGG	1 cut(s)	195
SchI	GAGTC	8 cut(s)	312, 342, 570, 571, 735, 863, 1254, 1366
ScrFI	CCNGG	4 cut(s)	274, 478, 720, 999
SdaI	CCTGCAGG	1 cut(s)	195
SduI	GDGCHC	1 cut(s)	669
SexAI	ACCWGGT	1 cut(s)	997
SfaNI	GCATC	6 cut(s)	528, 919, 920, 1132, 1228, 1429
SfcI	CTRYAG	4 cut(s)	191, 897, 1323, 1413
SfiI	GGCCNNNNNGGCC	1 cut(s)	203
SfoI	GGCGCC	1 cut(s)	234
Sfr303I	CCGCGG	1 cut(s)	204
SgrBI	CCGCGG	1 cut(s)	204
SinI	GGWCC	2 cut(s)	40, 716
SphI	GCATGC	1 cut(s)	1118
Sse8387I	CCTGCAGG	1 cut(s)	195
SspDI	GGCGCC	1 cut(s)	232
SspMI	CTAG	4 cut(s)	843, 1109, 1257, 1401
StyD4I	CCNGG	4 cut(s)	272, 476, 718, 997
TaaI	ACNGT	2 cut(s)	1252, 1285
TaiI	ACGT	3 cut(s)	270, 1179, 1186
TaqI	TCGA	4 cut(s)	81, 161, 264, 1090
TaqII	GACCGA	2 cut(s)	345, 377
TatI	WGTACW	1 cut(s)	905
TauI	GCSGC	8 cut(s)	200, 203, 206, 230, 233, 288, 291, 461
TfiI	GAWTC	2 cut(s)	238, 453
Tru1I	TTAA	4 cut(s)	378, 938, 989, 1407
Tru9I	TTAA	4 cut(s)	378, 938, 989, 1407
TscAI	CASTG	2 cut(s)	826, 1047
TseFI	GTSAC	2 cut(s)	487, 973
TseI	GCWGC	8 cut(s)	154, 186, 259, 371, 704, 890, 893, 1412
Tsp45I	GTSAC	2 cut(s)	487, 973
TspDTI	ATGAA	3 cut(s)	20, 1052, 1462
TspGWI	ACGGA	2 cut(s)	458, 506
TspRI	CASTG	2 cut(s)	826, 1047
Tth111I	GACNNNGTC	1 cut(s)	332
Van91I	CCANNNNNTGG	2 cut(s)	343, 1004
VpaK11BI	GGWCC	2 cut(s)	40, 716
XapI	RAATTY	4 cut(s)	633, 876, 1352, 1460
XceI	RCATGY	2 cut(s)	358, 1118
XmiI	GTMKAC	2 cut(s)	81, 1173
XmnI	GAANNNNTTC	2 cut(s)	879, 1059
XspI	CTAG	4 cut(s)	843, 1109, 1257, 1401
ZraI	GACGTC	1 cut(s)	268

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.