Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009089

Physical Location & Seq

Genomic Coordinates Reverse (-)

3379678 .. 3385456

Gene Structure 5779 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1049400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2664 bp

ATGATAAACAGTATGCTGAGGCTCTCTCTCTCCTCTGACCCCAAGCTCCTCTTCAGCTACACCACCAACTCCTCCAGTGTTGACCGCTTCGCCTTCAACTTCAAGCCCCACCTCCCGCAACGCCGCCGTTCCAACCACCTCCTCCGGCCCCAATCAATCCCTAATTTCACCCTCCGCAGCTCCCTCCAGACCTCCGCCGACGCCGCAGCGCCGCTTCAGCAAGTCCAGAATGATCCACCCGCCGAGGCATCGGTCCTGCTCGATGTCTCCGGGATGATGTGCGGCGGCTGCGTCTCGCGAGTCAAATCTGTTCTCTCCGCCGACGACCGAGTCGACTCGGTGGCGGTCAACTTGCTGACCGAAACGGCGGCGATCAAGCTGAGGCCGGAGGTCGCCGCCGATGTCGCGGCGGAGAGCCTGGCGGGGAGGCTGACAGAGTGCGGATTCGCTTCCAAGAGGAGGGCTTCCGGGATGGGAGTGGCGGAGAGCGTGAGGAAGTGGAAGGAGACGGTGAGGAAGAAGGAGGAGATGCTGGTGAAGAGCAGGAACAGAGTGATTTTAGCTTGGACTTTAGTGGCATTGTGCTGCGGATCGCATGCTTCTCACATTTTGCATTCTCTTGGAATTCATGCTGCTCATGGATCGTTTTGGGAGCTGCTGCATAATTCGTATGTAAAGGCGGGTTTAGCTACCGGGGCTTTGTTGGGACCTGGCCGAGACTTGCTTTTTGATGGTCTGAGAGCCTTCAAGAAAGGAGCACCAAACATGAATTCTCTTGTGGGATTCGGATCCCTCGCAGCTTTTACAATTAGTGCGGTCTCGCTTCTCAACCCTGGGCTTCAATGGGATGCAGCGTTCTTCGATGAGCCGGTCATGCTTCTTGGTTTTGTGCTCCTAGGACGTTCTCTCGAGGAAAGGGCAAGGATCAAGGCATCTAGTGATATGAATGAACTCTTGTCACTGATAAACACACAAGCAAGACTTGTAATTGGTTCCTCGGAAAATGATTCCTCCAGCAACAGTGTACTTTTCTCTGATGCAATATGCGTTGAAGTCCCAACTGACGATATTCGGGTTGGAGACTCTGTGTTGGTTTTGCCAGGAGAAACCATACCTGTGGATGGAAGAGTTGTGGCTGGAAGAAGTGTTGTCGACGAATCCATGCTCACAGGAGAATCACTTCCTGTATTCAAGGAAAAAGACCTTACTGTCTCAGCTGGAACCATAAACTGGGATGGTCCTCTGCGGGTTGAAGCATCTTCAACTGGCTCCAATTCAATGATATCCAAGATCGTTCGCATGGTTGAGGATGCTCAAGGACATGAAGCACCCATACAAAGGCTTGCTGATTCAATAGCTGGACCTTTTGTATACAGTATAATGACTCTATCAGCTACAACATTTGCTTTTTGGTACTACTTTGGAACACAAATTTTTCCAGATGTTTTGCTCAACGATATTGCTGGGCCAGATGGAGATCCATTGCTGTTGAGCTTGAAACTTGCTGTTGATGTCTTGGTTGTTTCTTGCCCTTGTGCACTGGGTCTTGCCACACCTACAGCAATCCTAGTTGGGACTTCACTCGGAGCAAGGCAAGGACTCCTTGTTAGAGGAGCAGATGTGCTTGAACGCTTAGCCAACGTAGATTATATTGCTTTAGATAAGACAGGAACTCTTACAGAAGGAAAACCCACTGTTTCTGGTATTGCATCTTTCATGTATGAAGAGTTGGAAATTCTTCAAATTGCTGCTGCAGTGGAGAATACAGCATCACATCCAATTGCAAAGGCTATCTTAAATAAAGCAAAATCATTGAATATGAGTATCCCAGTCACGAGAAGGCAATTAACAGAACCGGGTTTTGGAACTTTAGCAGAAGTCGATGGCCGATTGGTTGCAGTTGGTAGCTTAGAATGGGTCCGTGAACGGTTCCAGGCAAAAGCCAATGTGTCTGATCTTTTGAATCTAGAACATACTGTGCGCCAATCATCAGAAGGCATAACACCTTCAAGTTATTCAAAAACTATTGTATATGTTGGCCGTGAAGGAGAAGGCATCATCGGTGCTATTGCAATATCTGATAGCCTGCGTCATGATGCTGAGTTTACTGTAAATAGACTCCAGCAGAAGGGTATCCAAACTGTCCTCGTTTCTGGAGACAGGGAAGAGGCAGTTGCAACTATAGCGAAAGCTGTTGGAATAGAAAATGAATTTGTCAAATCATCATTGACTCCACAGGGAAAATCTGGAGCTATTTCTAGTCTTAAAGATGCAGGACATCACGTTGCAATGGTTGGCGATGGCATAAATGACGCACCATCTTTGGCACTTGCGGACGTTGGGATTGCCCTACAGATTGAGGGGCAAGAAAATGCCGCTTCAAATGCTGCATCTATTATACTTCTTGGAAACAAATTATCACAAGTTGTAGATGCCCTGGAACTGTCACAGGCAACAATGGCAAAAGTGTATCAAAATTTATCTTGGGCAATCGCATATAACCTATTTGCCATCCCCATTGCTGCCGGAGTACTGCTTCCTCAATATGATTTTGCCATGACACCATCACTCTCCGGCGGGATGATGGCCTTGAGCTCAATATTTGTAGTTTCCAATTCATTGCTTCTACAGCTCCATAAATCCGACGGGAGTAGAAAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0035434 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

888

Amino Acids

94.39

Weight (kDa)

5.71

Isoelectric Point (pI)

32.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	87 - 149	4.2e-10	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	344 - 436	1.4e-27	P-type ATPase actuator domain
Hydrolase	PF00702	549 - 779	2.4e-25	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21930 AT5G21930 AT5G21930 AT5G21930
fragaria_vesca	FvH4_2g30930 FvH4_2g30930 FvH4_2g30930
malus_domestica	MD15G1049400.v1.1
prunus_persica	Prupe.1G403700_v2.0.a1 Prupe.1G403700_v2.0.a1
pyrus_communis	pycom15g04820
rosa_chinensis	RchiOBHm_Chr6g0311921
rosa_multiflora	Rmu_ssc0000057.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157220
rosa_rugosa	Rorug06G0391300 Rorug06G0391400 Rorug06G0391400
rosa_samantha	Rh6AG507800 Rh6BG519000 Rh6CG524700 Rh6DG510300
rosa_wichuraiana	Rw6G044200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	329, 1208
AccB7I	CCANNNNNTGG	1 cut(s)	1230
AccI	GTMKAC	3 cut(s)	333, 1152, 1371
AccII	CGCG	2 cut(s)	298, 407
AclWI	GGATC	7 cut(s)	227, 598, 649, 783, 796, 932, 1472
AcoI	YGGCCR	3 cut(s)	712, 1885, 2038
AcsI	RAATTY	6 cut(s)	624, 769, 1431, 1734, 2210, 2476
AcuI	CTGAAG	2 cut(s)	37, 200
AcyI	GRCGYC	1 cut(s)	201
AfaI	GTAC	3 cut(s)	1026, 1415, 2532
AfiI	CCNNNNNNNGG	6 cut(s)	459, 1121, 1230, 1338, 1862, 2128
AjnI	CCWGG	6 cut(s)	417, 709, 832, 1099, 1932, 2436
AjuI	GAANNNNNNNTTGG	6 cut(s)	35, 67, 754, 786, 1845, 1877
AloI	GAACNNNNNNTCC	2 cut(s)	839, 871
Alw21I	GWGCWC	4 cut(s)	760, 894, 1540, 2597
Alw26I	GTCTC	8 cut(s)	271, 298, 500, 711, 823, 1074, 1216, 2151
Alw44I	GTGCAC	1 cut(s)	1536
AlwI	GGATC	7 cut(s)	227, 598, 649, 783, 796, 932, 1472
Ama87I	CYCGRG	1 cut(s)	908
AoxI	GGCC	7 cut(s)	146, 383, 712, 1466, 1885, 2038, 2586
ApaLI	GTGCAC	1 cut(s)	1536
ApoI	RAATTY	6 cut(s)	624, 769, 1431, 1734, 2210, 2476
ArsI	GACNNNNNNTTYG	2 cut(s)	710, 742
Asp700I	GAANNNNTTC	2 cut(s)	1179, 1737
AspA2I	CCTAGG	1 cut(s)	895
AspLEI	GCGC	2 cut(s)	211, 1983
AspS9I	GGNCC	7 cut(s)	147, 253, 707, 1238, 1361, 1466, 1918
AsuC2I	CCSGG	4 cut(s)	271, 469, 694, 1857
AsuHPI	GGTGA	3 cut(s)	160, 523, 547
AvaI	CYCGRG	1 cut(s)	908
AvaII	GGWCC	5 cut(s)	253, 707, 1238, 1361, 1918
AvrII	CCTAGG	1 cut(s)	895
BaeGI	GKGCMC	1 cut(s)	1540
BamHI	GGATCC	1 cut(s)	788
BanII	GRGCYC	1 cut(s)	2597
BarI	GAAGNNNNNNTAC	2 cut(s)	1317, 1349
BauI	CACGAG	1 cut(s)	1834
Bbv12I	GWGCWC	4 cut(s)	760, 894, 1540, 2597
BbvCI	CCTCAGC	2 cut(s)	17, 380
BceAI	ACGGC	3 cut(s)	111, 381, 2025
BciT130I	CCWGG	6 cut(s)	419, 711, 834, 1101, 1934, 2438
BciVI	GTATCC	2 cut(s)	1835, 2144
BcnI	CCSGG	4 cut(s)	271, 469, 694, 1857
BcoDI	GTCTC	8 cut(s)	271, 298, 500, 711, 823, 1074, 1216, 2151
BfaI	CTAG	5 cut(s)	896, 936, 1568, 1967, 2259
BfmI	CTRYAG	5 cut(s)	1557, 1752, 2181, 2351, 2627
BfoI	RGCGCY	1 cut(s)	212
BfuI	GTATCC	2 cut(s)	1835, 2144
BlnI	CCTAGG	1 cut(s)	895
BlpI	GCTNAGC	1 cut(s)	1633
BmcAI	AGTACT	1 cut(s)	2532
Bme18I	GGWCC	5 cut(s)	253, 707, 1238, 1361, 1918
BmeT110I	CYCGRG	1 cut(s)	908
BmgT120I	GGNCC	7 cut(s)	147, 253, 707, 1238, 1361, 1466, 1918
BmiI	GGNNCC	8 cut(s)	149, 708, 790, 994, 1222, 1270, 1919, 1931
BmrI	ACTGGG	3 cut(s)	1240, 1550, 1823
BmuI	ACTGGG	3 cut(s)	1240, 1550, 1823
BplI	GAGNNNNNCTC	2 cut(s)	10, 42
BpmI	CTGGAG	6 cut(s)	58, 170, 997, 2105, 2175, 2268
Bpu10I	CCTNAGC	2 cut(s)	17, 380
Bpu1102I	GCTNAGC	1 cut(s)	1633
BpuEI	CTTGAG	2 cut(s)	1299, 2611
BpuMI	CCSGG	4 cut(s)	271, 469, 694, 1857
BsaBI	GATNNNNATC	2 cut(s)	787, 1476
BsaHI	GRCGYC	1 cut(s)	201
BsaI	GGTCTC	1 cut(s)	823
BsaJI	CCNNGG	7 cut(s)	243, 693, 832, 833, 895, 996, 2436
BsaXI	ACNNNNNCTCC	4 cut(s)	1071, 1101, 1605, 1635
Bsc4I	CCNNNNNNNGG	6 cut(s)	459, 1121, 1230, 1338, 1862, 2128
Bse118I	RCCGGY	1 cut(s)	868
Bse1I	ACTGG	5 cut(s)	75, 1235, 1270, 1545, 1829
Bse3DI	GCAATG	4 cut(s)	1481, 2295, 2517, 2618
Bse8I	GATNNNNATC	2 cut(s)	787, 1476
BseBI	CCWGG	6 cut(s)	419, 711, 834, 1101, 1934, 2438
BseDI	CCNNGG	7 cut(s)	243, 693, 832, 833, 895, 996, 2436
BseGI	GGATG	9 cut(s)	279, 477, 853, 1126, 1240, 1315, 1774, 2511, 2586
BseJI	GATNNNNATC	2 cut(s)	787, 1476
BseLI	CCNNNNNNNGG	6 cut(s)	459, 1121, 1230, 1338, 1862, 2128
BseMI	GCAATG	4 cut(s)	1481, 2295, 2517, 2618
BseMII	CTCAG	5 cut(s)	8, 371, 728, 1227, 2091
BseNI	ACTGG	5 cut(s)	75, 1235, 1270, 1545, 1829
BseRI	GAGGAG	7 cut(s)	22, 38, 61, 131, 472, 539, 1626
BseSI	GKGCMC	1 cut(s)	1540
BseYI	CCCAGC	1 cut(s)	1463
Bsh1236I	CGCG	2 cut(s)	298, 407
Bsh1285I	CGRYCG	1 cut(s)	328
BshFI	GGCC	7 cut(s)	148, 385, 714, 1468, 1887, 2040, 2588
BsiEI	CGRYCG	1 cut(s)	328
BsiHKAI	GWGCWC	4 cut(s)	760, 894, 1540, 2597
BsiHKCI	CYCGRG	1 cut(s)	908
BsiSI	CCGG	9 cut(s)	145, 270, 386, 468, 693, 869, 1856, 2526, 2574
BslFI	GGGAC	3 cut(s)	720, 1040, 1588
BslI	CCNNNNNNNGG	6 cut(s)	459, 1121, 1230, 1338, 1862, 2128
BsmAI	GTCTC	8 cut(s)	271, 298, 500, 711, 823, 1074, 1216, 2151
BsmBI	CGTCTC	2 cut(s)	298, 500
BsmFI	GGGAC	3 cut(s)	720, 1040, 1588
BsmI	GAATGC	1 cut(s)	613
BsnI	GGCC	7 cut(s)	148, 385, 714, 1468, 1887, 2040, 2588
Bso31I	GGTCTC	1 cut(s)	823
BsoBI	CYCGRG	1 cut(s)	908
Bsp1286I	GDGCHC	4 cut(s)	760, 894, 1540, 2597
Bsp143I	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
Bsp1720I	GCTNAGC	1 cut(s)	1633
Bsp68I	TCGCGA	1 cut(s)	298
BspANI	GGCC	7 cut(s)	148, 385, 714, 1468, 1887, 2040, 2588
BspCNI	CTCAG	5 cut(s)	9, 372, 729, 1226, 2092
BspFNI	CGCG	2 cut(s)	298, 407
BspHI	TCATGA	1 cut(s)	2092
BspLI	GGNNCC	8 cut(s)	149, 708, 790, 994, 1222, 1270, 1919, 1931
BspMAI	CTGCAG	1 cut(s)	1756
BspPI	GGATC	7 cut(s)	227, 598, 649, 783, 796, 932, 1472
BspQI	GCTCTTC	1 cut(s)	533
BspTNI	GGTCTC	1 cut(s)	823
BsrDI	GCAATG	4 cut(s)	1481, 2295, 2517, 2618
BsrFI	RCCGGY	1 cut(s)	868
BsrI	ACTGG	5 cut(s)	75, 1235, 1270, 1545, 1829
BssAI	RCCGGY	1 cut(s)	868
BssECI	CCNNGG	7 cut(s)	243, 693, 832, 833, 895, 996, 2436
BssMI	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
BssNAI	GTATAC	1 cut(s)	1372
BssNI	GRCGYC	1 cut(s)	201
BssSI	CACGAG	1 cut(s)	1834
BssT1I	CCWWGG	1 cut(s)	895
Bst1107I	GTATAC	1 cut(s)	1372
Bst2BI	CACGAG	1 cut(s)	1834
Bst2UI	CCWGG	6 cut(s)	419, 711, 834, 1101, 1934, 2438
Bst6I	CTCTTC	5 cut(s)	56, 533, 1120, 1719, 2160
BstACI	GRCGYC	1 cut(s)	201
BstC8I	GCNNGC	3 cut(s)	597, 1344, 2087
BstDEI	CTNAG	7 cut(s)	17, 380, 737, 1213, 1633, 1909, 2100
BstF5I	GGATG	9 cut(s)	279, 477, 853, 1126, 1240, 1315, 1774, 2511, 2586
BstFNI	CGCG	2 cut(s)	298, 407
BstH2I	RGCGCY	1 cut(s)	212
BstHHI	GCGC	2 cut(s)	211, 1983
BstKTI	GATC	9 cut(s)	235, 375, 593, 644, 791, 927, 1293, 1480, 1957
BstMAI	GTCTC	8 cut(s)	271, 298, 500, 711, 823, 1074, 1216, 2151
BstMBI	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
BstMCI	CGRYCG	1 cut(s)	328
BstNI	CCWGG	6 cut(s)	419, 711, 834, 1101, 1934, 2438
BstNSI	RCATGY	1 cut(s)	599
BstSFI	CTRYAG	5 cut(s)	1557, 1752, 2181, 2351, 2627
BstSLI	GKGCMC	1 cut(s)	1540
BstUI	CGCG	2 cut(s)	298, 407
BstX2I	RGATCY	2 cut(s)	788, 1477
BstXI	CCANNNNNNTGG	1 cut(s)	1117
BstYI	RGATCY	2 cut(s)	788, 1477
BstZ17I	GTATAC	1 cut(s)	1372
BsuI	GTATCC	2 cut(s)	1835, 2144
BsuRI	GGCC	7 cut(s)	148, 385, 714, 1468, 1887, 2040, 2588
BtgZI	GCGATG	1 cut(s)	2313
BtsCI	GGATG	9 cut(s)	279, 477, 853, 1126, 1240, 1315, 1774, 2511, 2586
BtsI	GCAGTG	1 cut(s)	1761
BtsIMutI	CAGTG	6 cut(s)	82, 959, 1027, 1538, 1692, 1761
BtuMI	TCGCGA	1 cut(s)	298
Cac8I	GCNNGC	3 cut(s)	597, 1344, 2087
CciI	TCATGA	1 cut(s)	2092
CfoI	GCGC	2 cut(s)	211, 1983
Cfr10I	RCCGGY	1 cut(s)	868
Cfr13I	GGNCC	7 cut(s)	147, 253, 707, 1238, 1361, 1466, 1918
CseI	GACGC	4 cut(s)	209, 280, 2078, 2321
Csp6I	GTAC	3 cut(s)	1025, 1414, 2531
CviQI	GTAC	3 cut(s)	1025, 1414, 2531
DdeI	CTNAG	7 cut(s)	17, 380, 737, 1213, 1633, 1909, 2100
DpnI	GATC	9 cut(s)	234, 374, 592, 643, 790, 926, 1292, 1479, 1956
DpnII	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
DrdI	GACNNNNNNGTC	2 cut(s)	329, 1208
DseDI	GACNNNNNNGTC	2 cut(s)	329, 1208
EaeI	YGGCCR	3 cut(s)	712, 1885, 2038
Eam1104I	CTCTTC	5 cut(s)	56, 533, 1120, 1719, 2160
EarI	CTCTTC	5 cut(s)	56, 533, 1120, 1719, 2160
EciI	GGCGGA	4 cut(s)	184, 307, 425, 497
Ecl136II	GAGCTC	1 cut(s)	2595
Eco130I	CCWWGG	1 cut(s)	895
Eco24I	GRGCYC	1 cut(s)	2597
Eco31I	GGTCTC	1 cut(s)	823
Eco32I	GATATC	1 cut(s)	1284
Eco47I	GGWCC	5 cut(s)	253, 707, 1238, 1361, 1918
Eco53kI	GAGCTC	1 cut(s)	2595
Eco57I	CTGAAG	2 cut(s)	37, 200
Eco88I	CYCGRG	1 cut(s)	908
EcoICRI	GAGCTC	1 cut(s)	2595
EcoO109I	RGGNCCY	1 cut(s)	707
EcoRI	GAATTC	2 cut(s)	624, 769
EcoRII	CCWGG	6 cut(s)	417, 709, 832, 1099, 1932, 2436
EcoRV	GATATC	1 cut(s)	1284
EcoT14I	CCWWGG	1 cut(s)	895
EcoT38I	GRGCYC	1 cut(s)	2597
ErhI	CCWWGG	1 cut(s)	895
Esp3I	CGTCTC	2 cut(s)	298, 500
FalI	AAGNNNNNCTT	8 cut(s)	35, 67, 966, 998, 1587, 1619, 1778, 1810
FaqI	GGGAC	3 cut(s)	720, 1040, 1588
FauI	CCCGC	6 cut(s)	123, 247, 415, 673, 1239, 2570
FblI	GTMKAC	3 cut(s)	333, 1152, 1371
FokI	GGATG	9 cut(s)	286, 484, 860, 1133, 1247, 1322, 1761, 2498, 2593
FriOI	GRGCYC	1 cut(s)	2597
FspBI	CTAG	5 cut(s)	896, 936, 1568, 1967, 2259
GlaI	GCGC	2 cut(s)	210, 1982
GsaI	CCCAGC	1 cut(s)	1467
GsuI	CTGGAG	6 cut(s)	58, 170, 997, 2105, 2175, 2268
HaeII	RGCGCY	1 cut(s)	212
HaeIII	GGCC	7 cut(s)	148, 385, 714, 1468, 1887, 2040, 2588
HapII	CCGG	9 cut(s)	145, 270, 386, 468, 693, 869, 1856, 2526, 2574
HgaI	GACGC	4 cut(s)	209, 280, 2078, 2321
HhaI	GCGC	2 cut(s)	211, 1983
Hin1I	GRCGYC	1 cut(s)	201
Hin6I	GCGC	2 cut(s)	209, 1981
HinP1I	GCGC	2 cut(s)	209, 1981
HincII	GTYRAC	4 cut(s)	82, 334, 349, 1153
HindII	GTYRAC	4 cut(s)	82, 334, 349, 1153
HpaII	CCGG	9 cut(s)	145, 270, 386, 468, 693, 869, 1856, 2526, 2574
HphI	GGTGA	3 cut(s)	160, 523, 547
Hpy166II	GTNNAC	9 cut(s)	82, 334, 349, 1025, 1153, 1372, 1538, 1925, 2106
Hpy8I	GTNNAC	9 cut(s)	82, 334, 349, 1025, 1153, 1372, 1538, 1925, 2106
Hpy99I	CGWCG	4 cut(s)	203, 326, 1157, 2648
HpyCH4IV	ACGT	4 cut(s)	901, 1641, 2283, 2337
HpyF3I	CTNAG	7 cut(s)	17, 380, 737, 1213, 1633, 1909, 2100
HpySE526I	ACGT	4 cut(s)	901, 1641, 2283, 2337
Hsp92I	GRCGYC	1 cut(s)	201
HspAI	GCGC	2 cut(s)	209, 1981
Kzo9I	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
LguI	GCTCTTC	1 cut(s)	533
MaeI	CTAG	5 cut(s)	896, 936, 1568, 1967, 2259
MaeII	ACGT	4 cut(s)	901, 1641, 2283, 2337
MaeIII	GTNAC	3 cut(s)	957, 1831, 2445
MalI	GATC	9 cut(s)	234, 374, 592, 643, 790, 926, 1292, 1479, 1956
MboI	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
MfeI	CAATTG	1 cut(s)	1779
MflI	RGATCY	2 cut(s)	788, 1477
MhlI	GDGCHC	4 cut(s)	760, 894, 1540, 2597
MlyI	GAGTC	8 cut(s)	309, 329, 339, 1076, 1378, 1593, 2112, 2224
MmeI	TCCRAC	4 cut(s)	156, 1057, 1710, 2176
MroXI	GAANNNNTTC	2 cut(s)	1179, 1737
MseI	TTAA	3 cut(s)	1796, 1847, 2265
MslI	CAYNNNNRTG	1 cut(s)	1115
MspA1I	CMGCKG	1 cut(s)	1217
MspI	CCGG	9 cut(s)	145, 270, 386, 468, 693, 869, 1856, 2526, 2574
MunI	CAATTG	1 cut(s)	1779
Mva1269I	GAATGC	1 cut(s)	613
MvaI	CCWGG	6 cut(s)	419, 711, 834, 1101, 1934, 2438
MvnI	CGCG	2 cut(s)	298, 407
NciI	CCSGG	4 cut(s)	271, 469, 694, 1857
NdeII	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
NlaIV	GGNNCC	8 cut(s)	149, 708, 790, 994, 1222, 1270, 1919, 1931
NmeAIII	GCCGAG	2 cut(s)	268, 740
NmuCI	GTSAC	3 cut(s)	957, 1831, 2445
NruI	TCGCGA	1 cut(s)	298
NspI	RCATGY	1 cut(s)	599
PaeI	GCATGC	1 cut(s)	599
PaeR7I	CTCGAG	1 cut(s)	908
PagI	TCATGA	1 cut(s)	2092
PasI	CCCWGGG	1 cut(s)	833
PciSI	GCTCTTC	1 cut(s)	533
PcsI	WCGNNNNNNNCGW	1 cut(s)	330
PctI	GAATGC	1 cut(s)	613
PdmI	GAANNNNTTC	2 cut(s)	1179, 1737
PfeI	GAWTC	7 cut(s)	444, 783, 1007, 1157, 1175, 1349, 1963
PflFI	GACNNNGTC	1 cut(s)	329
PflMI	CCANNNNNTGG	1 cut(s)	1230
PfoI	TCCNGGA	2 cut(s)	269, 467
PleI	GAGTC	8 cut(s)	308, 329, 338, 1076, 1378, 1593, 2112, 2224
PpsI	GAGTC	8 cut(s)	308, 329, 338, 1076, 1378, 1593, 2112, 2224
PpuMI	RGGWCCY	1 cut(s)	707
Psp124BI	GAGCTC	1 cut(s)	2597
Psp5II	RGGWCCY	1 cut(s)	707
Psp6I	CCWGG	6 cut(s)	417, 709, 832, 1099, 1932, 2436
PspFI	CCCAGC	1 cut(s)	1463
PspGI	CCWGG	6 cut(s)	417, 709, 832, 1099, 1932, 2436
PspN4I	GGNNCC	8 cut(s)	149, 708, 790, 994, 1222, 1270, 1919, 1931
PspPI	GGNCC	7 cut(s)	147, 253, 707, 1238, 1361, 1466, 1918
PspPPI	RGGWCCY	1 cut(s)	707
PstI	CTGCAG	1 cut(s)	1756
PsuI	RGATCY	2 cut(s)	788, 1477
PsyI	GACNNNGTC	1 cut(s)	329
PvuII	CAGCTG	1 cut(s)	1217
RruI	TCGCGA	1 cut(s)	298
RsaI	GTAC	3 cut(s)	1026, 1415, 2532
RsaNI	GTAC	3 cut(s)	1025, 1414, 2531
RseI	CAYNNNNRTG	1 cut(s)	1115
SacI	GAGCTC	1 cut(s)	2597
SalI	GTCGAC	2 cut(s)	332, 1151
SapI	GCTCTTC	1 cut(s)	533
SaqAI	TTAA	3 cut(s)	1796, 1847, 2265
Sau3AI	GATC	9 cut(s)	232, 372, 590, 641, 788, 924, 1290, 1477, 1954
Sau96I	GGNCC	7 cut(s)	147, 253, 707, 1238, 1361, 1466, 1918
ScaI	AGTACT	1 cut(s)	2532
SchI	GAGTC	8 cut(s)	309, 329, 339, 1076, 1378, 1593, 2112, 2224
SduI	GDGCHC	4 cut(s)	760, 894, 1540, 2597
SfcI	CTRYAG	5 cut(s)	1557, 1752, 2181, 2351, 2627
Sfr274I	CTCGAG	1 cut(s)	908
SinI	GGWCC	5 cut(s)	253, 707, 1238, 1361, 1918
SlaI	CTCGAG	1 cut(s)	908
SmiMI	CAYNNNNRTG	1 cut(s)	1115
SmlI	CTYRAG	3 cut(s)	908, 1314, 2590
SmoI	CTYRAG	3 cut(s)	908, 1314, 2590
SphI	GCATGC	1 cut(s)	599
SspI	AATATT	1 cut(s)	2601
SspMI	CTAG	5 cut(s)	896, 936, 1568, 1967, 2259
SstI	GAGCTC	1 cut(s)	2597
StyI	CCWWGG	1 cut(s)	895
TaiI	ACGT	4 cut(s)	904, 1644, 2286, 2340
TaqI	TCGA	6 cut(s)	261, 333, 861, 909, 1152, 1881
TaqII	GACCGA	3 cut(s)	241, 342, 374
TatI	WGTACW	2 cut(s)	1024, 2530
TauI	GCSGC	9 cut(s)	126, 206, 214, 285, 288, 371, 398, 410, 2378
TfiI	GAWTC	7 cut(s)	444, 783, 1007, 1157, 1175, 1349, 1963
Tru1I	TTAA	3 cut(s)	1796, 1847, 2265
Tru9I	TTAA	3 cut(s)	1796, 1847, 2265
TscAI	CASTG	6 cut(s)	82, 966, 1027, 1545, 1699, 1761
TseFI	GTSAC	3 cut(s)	957, 1831, 2445
Tsp45I	GTSAC	3 cut(s)	957, 1831, 2445
TspDTI	ATGAA	9 cut(s)	617, 782, 959, 963, 1338, 1705, 1737, 2223, 2607
TspGWI	ACGGA	1 cut(s)	1910
TspRI	CASTG	6 cut(s)	82, 966, 1027, 1545, 1699, 1761
Tth111I	GACNNNGTC	1 cut(s)	329
Van91I	CCANNNNNTGG	1 cut(s)	1230
VneI	GTGCAC	1 cut(s)	1536
VpaK11BI	GGWCC	5 cut(s)	253, 707, 1238, 1361, 1918
XapI	RAATTY	6 cut(s)	624, 769, 1431, 1734, 2210, 2476
XbaI	TCTAGA	1 cut(s)	1966
XceI	RCATGY	1 cut(s)	599
XhoI	CTCGAG	1 cut(s)	908
XmaJI	CCTAGG	1 cut(s)	895
XmiI	GTMKAC	3 cut(s)	333, 1152, 1371
XmnI	GAANNNNTTC	2 cut(s)	1179, 1737
XspI	CTAG	5 cut(s)	896, 936, 1568, 1967, 2259
ZrmI	AGTACT	1 cut(s)	2532

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.