Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0009089

Physical Location & Seq

Genomic Coordinates Reverse (-)

997868 .. 1005422

Gene Structure 7555 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00157220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGATGAATACTATTCTCAATATCAGAGCCTCTCTCTCGCCGGACCCGAAGCTCCTGTTCAGCTACACCACCAACTCCAATGTCGACCGCTTCGCCTTCAACTTCAAGCCTCACCTCCCGATACGCCGTCGTTTCCCTCATCGCCGTCATCTCCGGCAGCAGTCGAACCCTAATTTCACTCCCTCCAGCTGCCTGCAGGCCGCCGCGGCCCCAAATCATATCGCCACGGCGGCGGCGCCAATTCAGGAGGAGGCATCGGTGCTGCTCGACGTCTCCGGGATGATGTGCGGCGGCTGTGTTTCGCGAGTCAAGTCGGTGCTGTCTGCGGACGACCGAGTCCACTCGGTGGCGGTCAACATGTTGACCGAGACGGCGGCGATTAAGCTGAGGCCGGAGGTTGAAGGTGGAGCGGAGGCCGTGGCGGAGAGCTTGGCCGGAAGGTTGACGGAGTGCGGATTCGCGGCGAGGAGGAGGGCTTCCGGGATGGGAGTGACGGAGAGTGTGAGGAAGTGGAAGGAGATGGTGAAGAAGAAGGAGGAGATGCTGGTGAAGAGTAGGAACCGAGTCATTTTGGCTTGGACTCTAGTGGCCTTGTGCTGTGGATCACACGCTTCCCACATTTTGCATTCTCTGGGAATTCACATTGCTCATGGATCGTATATGGATGTGCTCCATAATTCGTATGTGAAGGGTGGTTTGGCTATGGCAGCTTTGTTGGGACCCGGGCGAGGTTGGTACTACATTGGAACTCATATTTTTCCTGATGTCTTGCTCAATGATATTGCTGGGCCAGATGGAGATTCATTGCTGTTGAGCTTAAAACTAGCTGTGGATGTCTTGGTAGTTTCTTGCCCTTGTGCACTGGGTCTTGCAACACCCACAGCAATCCTAGTTGGCACTTCTCTTGGAGCAAGGCAAGGACTCCTTGTGAGAGGAGCAGATGTATTGGAACGCTTGGCCAGTATAGATCATATTGCTTTAGACAAGGTGATTTGCATTTACACAATCATGCTCACTCATACACATTGTACTGGCTCTGAAGAGATGAATCCATTTTACTCTTGCTCATATGTGAAGTTATCTTGCAATCTCACTCTAAGCTTCAACCTTCAGACAGGAACCCTCACTGAAGGAAAACCTGCTGTTTCTAGCATTGCTTCTTTCAAGTATAAAGACTCGGAAATTCTTCAAATAGCTGCTGCTGTAGAGAGTACAGCATCACATCCAATTGCTAATGCTATCTTAAACAAAGCCAAATCATTGGAGTTGAGTATCCCAGTCACAAAAAGGCAATTAACAGAACCAGGTTTTGGAACATTAGCAGAAGTAGATGGCCTTTTGGTTGCAGTGGGTTCTTTGGAATGGGTTGATGAGCGTTTCCAGAGAAGAACAGATCGATCTGACATTTCAAACCTACAGCATGCCGTGCATCAATCATCAGAAGGCATAACACCCTCAAATTATTCAAAAACAATTGTATATGTTGGACGTGAAGGGGAAGGTATCATCGGTGCTATTGCAATATCCGACAGCCTGCGGGAAGATGCTGAGTTTACTGTAACTAGACTCCAGCAGAAGGGTATCAGAACTGTCCTGTTTTCTGGGGACAGGGAAGAGGCAGTTGCAACTATAGCAAAATCAGTTGGAATAGAAAAGGAATTTATCAAGTCATCATTGACTCCACAGGGAAAATCTGCAGCTATTTCTAGTCTTAAAGCTGCAGGGCATCATGTTGCAATGGTTGGTGATGGCATAAATGATGCACCTTCTTTGGCTCTTGCCGACGTTGGGATTGCTCTTCAGATTGAGGGACAAGAAAATGCTGCTTCAAATGCTGCATCGATTATACTACTTGGAAACAAATTATCACAAGTTGTAGATGCACTGGAACTCGCACAGGCAACAATGGGAAAAGTGTATCAAAATTTATCTTGGGCTGTTGCATACAACGTAATTGCCATCCCCATTGCTGCTGGAGTACTGCTTCCTCAATACGATTTTGCCATGACACCATCACTTTCTGGTGGGATGATGGCCTTGAGCTCGATATTTGTAGTAACCAACTCTTTGATTCTACAGCTTCATAAGTCTGAAAGTGCTAGGAAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0035434 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

703

Amino Acids

75.17

Weight (kDa)

7.63

Isoelectric Point (pI)

41.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	89 - 133	2.3e-09	Heavy-metal-associated domain
Hydrolase	PF00702	372 - 595	1.5e-22	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21930 AT5G21930 AT5G21930 AT5G21930
fragaria_vesca	FvH4_2g30930 FvH4_2g30930 FvH4_2g30930
malus_domestica	MD15G1049400.v1.1
prunus_persica	Prupe.1G403700_v2.0.a1 Prupe.1G403700_v2.0.a1
pyrus_communis	pycom15g04820
rosa_chinensis	RchiOBHm_Chr6g0311921
rosa_multiflora	Rmu_ssc0000057.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157220
rosa_rugosa	Rorug06G0391300 Rorug06G0391400 Rorug06G0391400
rosa_samantha	Rh6AG507800 Rh6BG519000 Rh6CG524700 Rh6DG510300
rosa_wichuraiana	Rw6G044200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	335
AatII	GACGTC	1 cut(s)	273
Acc36I	ACCTGC	1 cut(s)	1147
AccB1I	GGYRCC	1 cut(s)	235
AccB7I	CCANNNNNTGG	2 cut(s)	346, 1310
AccBSI	CCGCTC	1 cut(s)	410
AccI	GTMKAC	1 cut(s)	84
AccII	CGCG	3 cut(s)	206, 304, 461
AclWI	GGATC	2 cut(s)	610, 661
AcoI	YGGCCR	2 cut(s)	432, 957
AcsI	RAATTY	4 cut(s)	636, 1182, 1658, 1924
AcuI	CTGAAG	4 cut(s)	1059, 1094, 1149, 1784
AcyI	GRCGYC	2 cut(s)	236, 270
AdeI	CACNNNGTG	1 cut(s)	346
AfaI	GTAC	4 cut(s)	737, 1030, 1213, 1980
AfiI	CCNNNNNNNGG	5 cut(s)	346, 728, 1310, 1576, 1788
AflIII	ACRYGT	1 cut(s)	357
AgsI	TTSAA	9 cut(s)	100, 106, 401, 1105, 1165, 1190, 1410, 1467, 1830
AjiI	CACGTC	1 cut(s)	1490
AjnI	CCWGG	1 cut(s)	1303
AjuI	GAANNNNNNNTTGG	4 cut(s)	680, 712, 1293, 1325
Alw21I	GWGCWC	3 cut(s)	672, 862, 2045
Alw26I	GTCTC	2 cut(s)	277, 362
Alw44I	GTGCAC	1 cut(s)	858
AlwI	GGATC	2 cut(s)	610, 661
Ama87I	CYCGRG	1 cut(s)	722
ApaLI	GTGCAC	1 cut(s)	858
ApoI	RAATTY	4 cut(s)	636, 1182, 1658, 1924
Asp700I	GAANNNNTTC	1 cut(s)	1185
AspLEI	GCGC	1 cut(s)	238
AspS9I	GGNCC	4 cut(s)	43, 208, 719, 788
AsuC2I	CCSGG	4 cut(s)	277, 481, 723, 724
AsuHPI	GGTGA	5 cut(s)	104, 535, 559, 1000, 1757
AvaI	CYCGRG	1 cut(s)	722
AvaII	GGWCC	2 cut(s)	43, 719
BaeGI	GKGCMC	1 cut(s)	862
BalI	TGGCCA	1 cut(s)	959
BanI	GGYRCC	1 cut(s)	235
BanII	GRGCYC	1 cut(s)	2045
Bbv12I	GWGCWC	3 cut(s)	672, 862, 2045
BbvCI	CCTCAGC	1 cut(s)	386
BccI	CCATC	8 cut(s)	478, 514, 788, 1325, 1742, 1967, 2020, 2026
BceAI	ACGGC	6 cut(s)	111, 129, 243, 387, 401, 1409
BciT130I	CCWGG	1 cut(s)	1305
BciVI	GTATCC	1 cut(s)	1283
BcnI	CCSGG	4 cut(s)	277, 481, 723, 724
BcoDI	GTCTC	2 cut(s)	277, 362
BfaI	CTAG	7 cut(s)	584, 824, 890, 1149, 1563, 1707, 2100
BfmI	CTRYAG	7 cut(s)	194, 1203, 1415, 1629, 1695, 1719, 2075
BfoI	RGCGCY	1 cut(s)	239
BfuAI	ACCTGC	1 cut(s)	1147
BfuI	GTATCC	1 cut(s)	1283
BglI	GCCNNNNNGGC	2 cut(s)	206, 230
BmcAI	AGTACT	1 cut(s)	1980
Bme1390I	CCNGG	5 cut(s)	277, 481, 723, 724, 1305
Bme18I	GGWCC	2 cut(s)	43, 719
BmeT110I	CYCGRG	1 cut(s)	722
BmgBI	CACGTC	1 cut(s)	1490
BmgT120I	GGNCC	4 cut(s)	43, 208, 719, 788
BmiI	GGNNCC	7 cut(s)	45, 210, 237, 560, 720, 721, 1120
BmrFI	CCNGG	5 cut(s)	277, 481, 723, 724, 1305
BmrI	ACTGGG	2 cut(s)	872, 1271
BmsI	GCATC	9 cut(s)	263, 531, 1226, 1438, 1534, 1735, 1750, 1847, 1870
BmuI	ACTGGG	2 cut(s)	872, 1271
BplI	GAGNNNNNCTC	2 cut(s)	18, 50
BpmI	CTGGAG	3 cut(s)	169, 1553, 1995
Bpu10I	CCTNAGC	1 cut(s)	386
BpuEI	CTTGAG	1 cut(s)	2059
BpuMI	CCSGG	4 cut(s)	277, 481, 723, 724
Bsa29I	ATCGAT	2 cut(s)	1396, 1841
BsaHI	GRCGYC	2 cut(s)	236, 270
BsaJI	CCNNGG	4 cut(s)	204, 225, 417, 722
BsaXI	ACNNNNNCTCC	2 cut(s)	927, 957
Bsc4I	CCNNNNNNNGG	5 cut(s)	346, 728, 1310, 1576, 1788
Bse1I	ACTGG	5 cut(s)	867, 960, 1036, 1277, 1890
Bse3DI	GCAATG	5 cut(s)	642, 803, 1152, 1743, 1965
BseBI	CCWGG	1 cut(s)	1305
BseCI	ATCGAT	2 cut(s)	1396, 1841
BseDI	CCNNGG	4 cut(s)	204, 225, 417, 722
BseGI	GGATG	7 cut(s)	285, 489, 670, 838, 1222, 1959, 2034
BseLI	CCNNNNNNNGG	5 cut(s)	346, 728, 1310, 1576, 1788
BseMI	GCAATG	5 cut(s)	642, 803, 1152, 1743, 1965
BseMII	CTCAG	2 cut(s)	377, 1539
BseNI	ACTGG	5 cut(s)	867, 960, 1036, 1277, 1890
BseRI	GAGGAG	5 cut(s)	263, 481, 484, 551, 948
BseSI	GKGCMC	1 cut(s)	862
BseYI	CCCAGC	1 cut(s)	785
Bsh1236I	CGCG	3 cut(s)	206, 304, 461
Bsh1285I	CGRYCG	2 cut(s)	88, 334
BshNI	GGYRCC	1 cut(s)	235
BshVI	ATCGAT	2 cut(s)	1396, 1841
BsiEI	CGRYCG	2 cut(s)	88, 334
BsiHKAI	GWGCWC	3 cut(s)	672, 862, 2045
BsiHKCI	CYCGRG	1 cut(s)	722
BsiSI	CCGG	7 cut(s)	41, 154, 276, 392, 435, 480, 723
BslFI	GGGAC	3 cut(s)	732, 1619, 1824
BslI	CCNNNNNNNGG	5 cut(s)	346, 728, 1310, 1576, 1788
BsmAI	GTCTC	2 cut(s)	277, 362
BsmBI	CGTCTC	2 cut(s)	277, 362
BsmFI	GGGAC	3 cut(s)	732, 1619, 1824
BsmI	GAATGC	1 cut(s)	625
BsoBI	CYCGRG	1 cut(s)	722
Bsp1286I	GDGCHC	3 cut(s)	672, 862, 2045
Bsp143I	GATC	5 cut(s)	602, 653, 967, 1393, 1397
Bsp68I	TCGCGA	1 cut(s)	304
BspCNI	CTCAG	2 cut(s)	378, 1540
BspDI	ATCGAT	2 cut(s)	1396, 1841
BspFNI	CGCG	3 cut(s)	206, 304, 461
BspLI	GGNNCC	7 cut(s)	45, 210, 237, 560, 720, 721, 1120
BspMAI	CTGCAG	3 cut(s)	198, 1699, 1723
BspMI	ACCTGC	1 cut(s)	1147
BspPI	GGATC	2 cut(s)	610, 661
BspQI	GCTCTTC	1 cut(s)	1803
BspT107I	GGYRCC	1 cut(s)	235
BsrBI	CCGCTC	1 cut(s)	410
BsrDI	GCAATG	5 cut(s)	642, 803, 1152, 1743, 1965
BsrI	ACTGG	5 cut(s)	867, 960, 1036, 1277, 1890
BssECI	CCNNGG	4 cut(s)	204, 225, 417, 722
BssMI	GATC	5 cut(s)	602, 653, 967, 1393, 1397
BssNI	GRCGYC	2 cut(s)	236, 270
Bst2UI	CCWGG	1 cut(s)	1305
Bst4CI	ACNGT	2 cut(s)	1558, 1591
Bst6I	CTCTTC	4 cut(s)	545, 1035, 1608, 1803
BstACI	GRCGYC	2 cut(s)	236, 270
BstAPI	GCANNNNNTGC	1 cut(s)	1426
BstC8I	GCNNGC	4 cut(s)	194, 198, 1422, 1535
BstDEI	CTNAG	3 cut(s)	386, 1097, 1548
BstDSI	CCRYGG	3 cut(s)	204, 225, 417
BstF5I	GGATG	7 cut(s)	285, 489, 670, 838, 1222, 1959, 2034
BstFNI	CGCG	3 cut(s)	206, 304, 461
BstH2I	RGCGCY	1 cut(s)	239
BstHHI	GCGC	1 cut(s)	238
BstKTI	GATC	5 cut(s)	605, 656, 970, 1396, 1400
BstMAI	GTCTC	2 cut(s)	277, 362
BstMBI	GATC	5 cut(s)	602, 653, 967, 1393, 1397
BstMCI	CGRYCG	2 cut(s)	88, 334
BstMWI	GCNNNNNNNGC	6 cut(s)	206, 230, 707, 1426, 1631, 1832
BstNI	CCWGG	1 cut(s)	1305
BstNSI	RCATGY	2 cut(s)	361, 1424
BstSCI	CCNGG	5 cut(s)	275, 479, 721, 722, 1303
BstSFI	CTRYAG	7 cut(s)	194, 1203, 1415, 1629, 1695, 1719, 2075
BstSLI	GKGCMC	1 cut(s)	862
BstUI	CGCG	3 cut(s)	206, 304, 461
BstXI	CCANNNNNNTGG	1 cut(s)	1261
Bsu15I	ATCGAT	2 cut(s)	1396, 1841
BsuI	GTATCC	1 cut(s)	1283
BsuTUI	ATCGAT	2 cut(s)	1396, 1841
BtgI	CCRYGG	3 cut(s)	204, 225, 417
BtgZI	GCGATG	1 cut(s)	125
BtrI	CACGTC	1 cut(s)	1490
BtsCI	GGATG	7 cut(s)	285, 489, 670, 838, 1222, 1959, 2034
BtsI	GCAGTG	1 cut(s)	1353
BtsIMutI	CAGTG	4 cut(s)	860, 1125, 1353, 1883
BtuMI	TCGCGA	1 cut(s)	304
BveI	ACCTGC	1 cut(s)	1147
Cac8I	GCNNGC	4 cut(s)	194, 198, 1422, 1535
CfoI	GCGC	1 cut(s)	238
Cfr13I	GGNCC	4 cut(s)	43, 208, 719, 788
Cfr42I	CCGCGG	1 cut(s)	207
Cfr9I	CCCGGG	1 cut(s)	722
ClaI	ATCGAT	2 cut(s)	1396, 1841
CsiI	ACCWGGT	1 cut(s)	1303
Csp6I	GTAC	4 cut(s)	736, 1029, 1212, 1979
CviAII	CATG	6 cut(s)	358, 650, 1009, 1421, 1730, 2005
CviQI	GTAC	4 cut(s)	736, 1029, 1212, 1979
DdeI	CTNAG	3 cut(s)	386, 1097, 1548
DinI	GGCGCC	1 cut(s)	237
DpnI	GATC	5 cut(s)	604, 655, 969, 1395, 1399
DpnII	GATC	5 cut(s)	602, 653, 967, 1393, 1397
DraIII	CACNNNGTG	1 cut(s)	346
DrdI	GACNNNNNNGTC	1 cut(s)	335
DseDI	GACNNNNNNGTC	1 cut(s)	335
EaeI	YGGCCR	2 cut(s)	432, 957
Eam1104I	CTCTTC	4 cut(s)	545, 1035, 1608, 1803
EarI	CTCTTC	4 cut(s)	545, 1035, 1608, 1803
EciI	GGCGGA	1 cut(s)	437
Ecl136II	GAGCTC	1 cut(s)	2043
Eco24I	GRGCYC	1 cut(s)	2045
Eco47I	GGWCC	2 cut(s)	43, 719
Eco53kI	GAGCTC	1 cut(s)	2043
Eco57I	CTGAAG	4 cut(s)	1059, 1094, 1149, 1784
Eco88I	CYCGRG	1 cut(s)	722
EcoICRI	GAGCTC	1 cut(s)	2043
EcoO109I	RGGNCCY	1 cut(s)	719
EcoRI	GAATTC	1 cut(s)	636
EcoRII	CCWGG	1 cut(s)	1303
EcoT38I	GRGCYC	1 cut(s)	2045
EgeI	GGCGCC	1 cut(s)	237
EheI	GGCGCC	1 cut(s)	237
Esp3I	CGTCTC	2 cut(s)	277, 362
FaeI	CATG	6 cut(s)	361, 653, 1012, 1424, 1733, 2008
FalI	AAGNNNNNCTT	2 cut(s)	909, 941
FaqI	GGGAC	3 cut(s)	732, 1619, 1824
FatI	CATG	6 cut(s)	357, 649, 1008, 1420, 1729, 2004
FauI	CCCGC	1 cut(s)	1530
FauNDI	CATATG	1 cut(s)	1069
FblI	GTMKAC	1 cut(s)	84
FokI	GGATG	7 cut(s)	292, 496, 677, 845, 1209, 1946, 2041
FriOI	GRGCYC	1 cut(s)	2045
FspBI	CTAG	7 cut(s)	584, 824, 890, 1149, 1563, 1707, 2100
GlaI	GCGC	1 cut(s)	237
GsaI	CCCAGC	1 cut(s)	789
GsuI	CTGGAG	3 cut(s)	169, 1553, 1995
HaeII	RGCGCY	1 cut(s)	239
HapII	CCGG	7 cut(s)	41, 154, 276, 392, 435, 480, 723
HhaI	GCGC	1 cut(s)	238
Hin1I	GRCGYC	2 cut(s)	236, 270
Hin1II	CATG	6 cut(s)	361, 653, 1012, 1424, 1733, 2008
Hin6I	GCGC	1 cut(s)	236
HinP1I	GCGC	1 cut(s)	236
HincII	GTYRAC	4 cut(s)	85, 355, 363, 444
HindII	GTYRAC	4 cut(s)	85, 355, 363, 444
HindIII	AAGCTT	1 cut(s)	1099
HpaII	CCGG	7 cut(s)	41, 154, 276, 392, 435, 480, 723
HphI	GGTGA	5 cut(s)	104, 535, 559, 1000, 1757
Hpy166II	GTNNAC	7 cut(s)	85, 340, 355, 363, 444, 860, 1554
Hpy188III	TCNNGA	5 cut(s)	118, 245, 303, 761, 1381
Hpy8I	GTNNAC	7 cut(s)	85, 340, 355, 363, 444, 860, 1554
Hpy99I	CGWCG	3 cut(s)	132, 272, 1787
HpyCH4III	ACNGT	2 cut(s)	1558, 1591
HpyCH4IV	ACGT	4 cut(s)	270, 1489, 1785, 1950
HpyF10VI	GCNNNNNNNGC	6 cut(s)	206, 230, 707, 1426, 1631, 1832
HpyF3I	CTNAG	3 cut(s)	386, 1097, 1548
HpySE526I	ACGT	4 cut(s)	270, 1489, 1785, 1950
Hsp92I	GRCGYC	2 cut(s)	236, 270
Hsp92II	CATG	6 cut(s)	361, 653, 1012, 1424, 1733, 2008
HspAI	GCGC	1 cut(s)	236
KasI	GGCGCC	1 cut(s)	235
KflI	GGGWCCC	1 cut(s)	719
KspI	CCGCGG	1 cut(s)	207
Kzo9I	GATC	5 cut(s)	602, 653, 967, 1393, 1397
LguI	GCTCTTC	1 cut(s)	1803
LmnI	GCTCC	5 cut(s)	57, 407, 675, 908, 935
LweI	GCATC	9 cut(s)	263, 531, 1226, 1438, 1534, 1735, 1750, 1847, 1870
MabI	ACCWGGT	1 cut(s)	1303
MaeI	CTAG	7 cut(s)	584, 824, 890, 1149, 1563, 1707, 2100
MaeII	ACGT	4 cut(s)	270, 1489, 1785, 1950
MaeIII	GTNAC	4 cut(s)	490, 1279, 1558, 2056
MalI	GATC	5 cut(s)	604, 655, 969, 1395, 1399
MbiI	CCGCTC	1 cut(s)	410
MboI	GATC	5 cut(s)	602, 653, 967, 1393, 1397
MboII	GAAGA	9 cut(s)	538, 541, 562, 1052, 1178, 1398, 1553, 1625, 1790
MfeI	CAATTG	2 cut(s)	1227, 1473
MhlI	GDGCHC	3 cut(s)	672, 862, 2045
MlsI	TGGCCA	1 cut(s)	959
MluNI	TGGCCA	1 cut(s)	959
Mly113I	GGCGCC	1 cut(s)	236
MlyI	GAGTC	8 cut(s)	315, 345, 573, 574, 915, 1169, 1560, 1672
MmeI	TCCRAC	3 cut(s)	1465, 1551, 1624
Mox20I	TGGCCA	1 cut(s)	959
MroXI	GAANNNNTTC	1 cut(s)	1185
MscI	TGGCCA	1 cut(s)	959
MseI	TTAA	5 cut(s)	381, 818, 1244, 1295, 1713
MslI	CAYNNNNRTG	1 cut(s)	1007
Msp20I	TGGCCA	1 cut(s)	959
MspA1I	CMGCKG	2 cut(s)	189, 206
MspI	CCGG	7 cut(s)	41, 154, 276, 392, 435, 480, 723
MspR9I	CCNGG	5 cut(s)	277, 481, 723, 724, 1305
MunI	CAATTG	2 cut(s)	1227, 1473
Mva1269I	GAATGC	1 cut(s)	625
MvaI	CCWGG	1 cut(s)	1305
MvnI	CGCG	3 cut(s)	206, 304, 461
MwoI	GCNNNNNNNGC	6 cut(s)	206, 230, 707, 1426, 1631, 1832
NarI	GGCGCC	1 cut(s)	236
NciI	CCSGG	4 cut(s)	277, 481, 723, 724
NdeI	CATATG	1 cut(s)	1069
NdeII	GATC	5 cut(s)	602, 653, 967, 1393, 1397
NlaIII	CATG	6 cut(s)	361, 653, 1012, 1424, 1733, 2008
NlaIV	GGNNCC	7 cut(s)	45, 210, 237, 560, 720, 721, 1120
NmuCI	GTSAC	2 cut(s)	490, 1279
NruI	TCGCGA	1 cut(s)	304
NspI	RCATGY	2 cut(s)	361, 1424
PaeI	GCATGC	1 cut(s)	1424
PciI	ACATGT	1 cut(s)	357
PciSI	GCTCTTC	1 cut(s)	1803
PcsI	WCGNNNNNNNCGW	1 cut(s)	44
PctI	GAATGC	1 cut(s)	625
PdmI	GAANNNNTTC	1 cut(s)	1185
PfeI	GAWTC	4 cut(s)	456, 800, 1048, 2071
PflFI	GACNNNGTC	1 cut(s)	335
PflMI	CCANNNNNTGG	2 cut(s)	346, 1310
PfoI	TCCNGGA	2 cut(s)	275, 479
PleI	GAGTC	8 cut(s)	314, 344, 572, 574, 915, 1169, 1560, 1672
PluTI	GGCGCC	1 cut(s)	239
PpsI	GAGTC	8 cut(s)	314, 344, 572, 574, 915, 1169, 1560, 1672
PpuMI	RGGWCCY	1 cut(s)	719
PscI	ACATGT	1 cut(s)	357
Psp124BI	GAGCTC	1 cut(s)	2045
Psp5II	RGGWCCY	1 cut(s)	719
Psp6I	CCWGG	1 cut(s)	1303
PspFI	CCCAGC	1 cut(s)	785
PspGI	CCWGG	1 cut(s)	1303
PspN4I	GGNNCC	7 cut(s)	45, 210, 237, 560, 720, 721, 1120
PspPI	GGNCC	4 cut(s)	43, 208, 719, 788
PspPPI	RGGWCCY	1 cut(s)	719
PstI	CTGCAG	3 cut(s)	198, 1699, 1723
PsyI	GACNNNGTC	1 cut(s)	335
PvuII	CAGCTG	1 cut(s)	189
RruI	TCGCGA	1 cut(s)	304
RsaI	GTAC	4 cut(s)	737, 1030, 1213, 1980
RsaNI	GTAC	4 cut(s)	736, 1029, 1212, 1979
RseI	CAYNNNNRTG	1 cut(s)	1007
SacI	GAGCTC	1 cut(s)	2045
SacII	CCGCGG	1 cut(s)	207
SalI	GTCGAC	1 cut(s)	83
SapI	GCTCTTC	1 cut(s)	1803
SaqAI	TTAA	5 cut(s)	381, 818, 1244, 1295, 1713
Sau3AI	GATC	5 cut(s)	602, 653, 967, 1393, 1397
Sau96I	GGNCC	4 cut(s)	43, 208, 719, 788
SbfI	CCTGCAGG	1 cut(s)	198
ScaI	AGTACT	1 cut(s)	1980
SchI	GAGTC	8 cut(s)	315, 345, 573, 574, 915, 1169, 1560, 1672
ScrFI	CCNGG	5 cut(s)	277, 481, 723, 724, 1305
SdaI	CCTGCAGG	1 cut(s)	198
SduI	GDGCHC	3 cut(s)	672, 862, 2045
SexAI	ACCWGGT	1 cut(s)	1303
SfaNI	GCATC	9 cut(s)	263, 531, 1226, 1438, 1534, 1735, 1750, 1847, 1870
SfcI	CTRYAG	7 cut(s)	194, 1203, 1415, 1629, 1695, 1719, 2075
SfiI	GGCCNNNNNGGCC	1 cut(s)	206
SfoI	GGCGCC	1 cut(s)	237
Sfr303I	CCGCGG	1 cut(s)	207
SgrBI	CCGCGG	1 cut(s)	207
SinI	GGWCC	2 cut(s)	43, 719
SmaI	CCCGGG	1 cut(s)	724
SmiMI	CAYNNNNRTG	1 cut(s)	1007
SmlI	CTYRAG	1 cut(s)	2038
SmoI	CTYRAG	1 cut(s)	2038
SphI	GCATGC	1 cut(s)	1424
Sse8387I	CCTGCAGG	1 cut(s)	198
SspDI	GGCGCC	1 cut(s)	235
SspMI	CTAG	7 cut(s)	584, 824, 890, 1149, 1563, 1707, 2100
SstI	GAGCTC	1 cut(s)	2045
StyD4I	CCNGG	5 cut(s)	275, 479, 721, 722, 1303
TaaI	ACNGT	2 cut(s)	1558, 1591
TaiI	ACGT	4 cut(s)	273, 1492, 1788, 1953
TaqI	TCGA	6 cut(s)	84, 164, 267, 1396, 1841, 2045
TaqII	GACCGA	2 cut(s)	348, 380
TatI	WGTACW	3 cut(s)	1028, 1211, 1978
TauI	GCSGC	9 cut(s)	203, 206, 209, 233, 236, 291, 294, 377, 464
TfiI	GAWTC	4 cut(s)	456, 800, 1048, 2071
Tru1I	TTAA	5 cut(s)	381, 818, 1244, 1295, 1713
Tru9I	TTAA	5 cut(s)	381, 818, 1244, 1295, 1713
TscAI	CASTG	4 cut(s)	867, 1132, 1353, 1890
TseFI	GTSAC	2 cut(s)	490, 1279
Tsp45I	GTSAC	2 cut(s)	490, 1279
TspDTI	ATGAA	4 cut(s)	20, 792, 1061, 2072
TspGWI	ACGGA	2 cut(s)	461, 509
TspMI	CCCGGG	1 cut(s)	722
TspRI	CASTG	4 cut(s)	867, 1132, 1353, 1890
Tth111I	GACNNNGTC	1 cut(s)	335
Van91I	CCANNNNNTGG	2 cut(s)	346, 1310
VneI	GTGCAC	1 cut(s)	858
VpaK11BI	GGWCC	2 cut(s)	43, 719
XapI	RAATTY	4 cut(s)	636, 1182, 1658, 1924
XceI	RCATGY	2 cut(s)	361, 1424
XmaI	CCCGGG	1 cut(s)	722
XmiI	GTMKAC	1 cut(s)	84
XmnI	GAANNNNTTC	1 cut(s)	1185
XspI	CTAG	7 cut(s)	584, 824, 890, 1149, 1563, 1707, 2100
ZraI	GACGTC	1 cut(s)	271
ZrmI	AGTACT	1 cut(s)	1980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.