Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0009089

Physical Location & Seq

Genomic Coordinates Forward (+)

67357357 .. 67363273

Gene Structure 5917 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G044200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGATGAATACTATTCTCAATATCAGAGCCTCTCTCTCGCCGGACCCGAAGCTCTTGTTCAGCTACACCACCAACTCCAATGTCGACCGCTTCGCCTTCAACTTCAAGCCTCACCTCCCGATACGCCGTCGTTTCCCTCATCGCCGCCATCTCCGGCAGCATTCGAACCCTAATTTCACTCTCTCCAGCTGCCTGCAGGCCGCCGCCGCCGCCCCAAATCATATCGCCACGGCGGCGGCGCCGATTCAGGAGGAGGCGTCGGTGCTGCTCGACGTCTCCGGGATGATGTGCGGCGGCTGCGTGTCGCGAGTCAAGTCGGTGCTGTCTGCTGACGACCGAGTCCACTCCGTGGCGGTCAACATGTTGACCGAGACGGCGGCGATTAAGCTGAGGCCGGAGGTTGAAGGTGGAGCGGAGGCCGTCGCCGAGAGCTTGGCCGGAAGGTTGACGGAGTGCGGATTCGCGGCGAGGAGGAGGGCTTCCGGGGTGGGAGTGACGGAGAGTGTGAGGAAGTGGAAGGAGATGGCGAAGAAGAAGGAGGAGATGCTGGTGAAGAGTAGGAACCGAGTCATTTTGGCTTGGACTCTGGTGGCCTTGTGCTGTGGATCACACGCTTCCCACATTTTGCATTCTCTGGGAATTCACATTGCTCATGGATCGTATATGGATGTGCTCCATAATTCCTATGTGAAGGGTGGTTTGGCTATGGCAGCTTTGTTGGGACCCGGACGAGACTTGCTTTTTGATGGTTTGAGGGCTTTCAAAAAAGGATCACCCAATATGAATTCTCTTGTGGGATTTGGATCCCTGGCTGCTTTTACAATTAGTGCGGTCTCTCTTCTTAACCCTGAACTTCAATGGGATGCAGCCTTCTTTGATGAGCCGGTCATGCTTCTTGGTTTTGTGCTCCTAGGACGTTCTCTTGAAGAAAGGGCAAGGATCAGGGCATCTAGTGATATGAACGAACTCTTGTCACTGATAAACACTCAATCAAGACTTGTAATTGCATCCTCAGAAAATGATTCCTCCAGTGACACTGTACTTGGCTCCGATGCAATTTGCATTGAGGTCCCAACTGATGATGTTCGTGTGGGAGATTCTGTGTTGATATTGCCAGGAGAAACTATACCCGTGGATGGAAGAGTTCTGGCTGGAAGAAGTGTTGTGGATGAGTCCATGCTCACAGGAGAATCGCTTCCTGTATTTAAGGAAAAAGAACTTACCGTCTCAGCTGGAACAATAAATTGGGATGGTCCTCTGAGAATTGAAGCAACTTCAACTGGCTCCAATTCAATGATTTCCAAGATTGTTCGCATGGTTGAGGATGCTCAAGGCCATGAAGCACCAATTCAAAGGCTTGCTGATTCAATAGCTGGACCTTTTGTATACACTATAATGACTCTCTCAGCTACAACATTTGCTTTTTGGTACTACATTGGAACTCATATTTTTCCTGATGTTTTGCTCAATGATATTGCTGGGCCAGATGGAGATTCATTGCTGTTGAGCTTGAAACTAGCTGTGGATGTCTTGGTAGTTTCTTGCCCTTGTGCACTGGGTCTTGCAACACCCACAGCAATCCTAGTTGGCACTTCTCTTGGAGCAAGGCAAGGACTCCTTGTGAGAGGAGCAGATGTATTGGAACGCTTGGCCAGTATAGATCATATTGCTTTAGACAAGACAGGAACCCTCACTGAAGGAAAACCTGCTGTTTCTAGCATTGCTTCTTTCAAGTATAAAGACTCGGAAATTCTTCAAATAGCTGCTGCTGTAGAGAGTACAGCATCACATCCAATTGCTGATGCTATCTTAAACAAAGCCAAATCATTGGAGTTGAGTATCCCAGTCACAAAAAGGCAATTAACCGAACCGGGTTTTGGAACATTAGCAGAAGTAGATGGCCTTTTGGTTGCAGTGGGTTCTTTGGAATGGGTTCATGAGCAGCATGCTGTGCATCAATCATCAGAAGGCATAACACCCTCAAATTATTCAAAAACAATTGTATATGTTGGACGTGAAGGGGAAGGTATCATCGGTGCTATAGCAATATCCGACAGCCTGCGGGAAGATGCTGAGTTTACTGTAACTAGACTCCAGCAGAAGGGTATCAGAACTGTCCTGTTTTCTGGGGACAGGGAAGAGGCAGTTGCAACTATAGCAAAATCAGTTGGAATAGAAAAGGAATTTATCAAGTCATCATTGACTCCACAGGGAAAATCTGGAGCTATTTCTAGTCTTAAAGCTGCAGGGCATCATGTTGCAATGGTTGGTGATGGCATAAATGATGCACCTTCTTTGGCTCTTGCCGACGTTGGGATTGCTCTTCAGATTGAGGGACAAGAAAATGCTGCTTCAAATGCTGCATCGATTATACTACTTGGAAACAAATTATCACAAGTTGTAGATGCACTGGAACTTGCACAGGCAACAATGGGAAAAGTGTATCAAAATTTATCTTGGGCCGTTGCATACAACGTAATTGCCATCCCCATTGCTGCTGGAGTACTGCTTCCTCAATACGATTTTGCCATGACACCATCACTTTCTGGTGGGATGATGGCCTTGAGCTCGATATTTGTAGTAACCAACTCCTTGCTTCTACAGCTTCATAAGTCTGAAAGTGCTAGGAAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0035434 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

878

Amino Acids

93.35

Weight (kDa)

5.91

Isoelectric Point (pI)

37.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	90 - 134	3e-09	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	350 - 441	1.7e-27	P-type ATPase actuator domain
Hydrolase	PF00702	553 - 770	6e-26	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21930 AT5G21930 AT5G21930 AT5G21930
fragaria_vesca	FvH4_2g30930 FvH4_2g30930 FvH4_2g30930
malus_domestica	MD15G1049400.v1.1
prunus_persica	Prupe.1G403700_v2.0.a1 Prupe.1G403700_v2.0.a1
pyrus_communis	pycom15g04820
rosa_chinensis	RchiOBHm_Chr6g0311921
rosa_multiflora	Rmu_ssc0000057.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157220
rosa_rugosa	Rorug06G0391300 Rorug06G0391400 Rorug06G0391400
rosa_samantha	Rh6AG507800 Rh6BG519000 Rh6CG524700 Rh6DG510300
rosa_wichuraiana	Rw6G044200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	338
AatII	GACGTC	1 cut(s)	276
Acc36I	ACCTGC	1 cut(s)	1714
AccB1I	GGYRCC	1 cut(s)	238
AccB7I	CCANNNNNTGG	1 cut(s)	349
AccBSI	CCGCTC	1 cut(s)	413
AccI	GTMKAC	2 cut(s)	84, 1386
AccII	CGCG	2 cut(s)	307, 464
AclWI	GGATC	6 cut(s)	613, 664, 778, 798, 811, 947
AcoI	YGGCCR	2 cut(s)	435, 1650
AcsI	RAATTY	5 cut(s)	639, 784, 1749, 2183, 2449
AcuI	CTGAAG	2 cut(s)	1716, 2309
AcyI	GRCGYC	3 cut(s)	239, 257, 273
AdeI	CACNNNGTG	1 cut(s)	349
AfaI	GTAC	4 cut(s)	1041, 1430, 1780, 2505
AfiI	CCNNNNNNNGG	5 cut(s)	349, 1136, 1877, 2101, 2313
AflIII	ACRYGT	1 cut(s)	360
AjiI	CACGTC	1 cut(s)	2015
AjnI	CCWGG	2 cut(s)	807, 1114
AjuI	GAANNNNNNNTTGG	6 cut(s)	683, 715, 1228, 1260, 1860, 1892
Alw21I	GWGCWC	4 cut(s)	675, 909, 1555, 2570
Alw26I	GTCTC	5 cut(s)	280, 365, 726, 838, 1231
Alw44I	GTGCAC	1 cut(s)	1551
AlwI	GGATC	6 cut(s)	613, 664, 778, 798, 811, 947
ApaLI	GTGCAC	1 cut(s)	1551
ApoI	RAATTY	5 cut(s)	639, 784, 1749, 2183, 2449
ArsI	GACNNNNNNTTYG	2 cut(s)	725, 757
Asp700I	GAANNNNTTC	3 cut(s)	1194, 1752, 1932
AspA2I	CCTAGG	1 cut(s)	910
AspLEI	GCGC	1 cut(s)	241
AspS9I	GGNCC	7 cut(s)	43, 722, 1069, 1253, 1376, 1481, 2460
AsuC2I	CCSGG	4 cut(s)	280, 484, 726, 1872
AsuHPI	GGTGA	4 cut(s)	104, 562, 765, 2282
AsuII	TTCGAA	1 cut(s)	164
AvaII	GGWCC	5 cut(s)	43, 722, 1069, 1253, 1376
AvrII	CCTAGG	1 cut(s)	910
BaeGI	GKGCMC	1 cut(s)	1555
BalI	TGGCCA	1 cut(s)	1652
BamHI	GGATCC	1 cut(s)	803
BanI	GGYRCC	1 cut(s)	238
BanII	GRGCYC	1 cut(s)	2570
Bbv12I	GWGCWC	4 cut(s)	675, 909, 1555, 2570
BbvCI	CCTCAGC	1 cut(s)	389
BceAI	ACGGC	5 cut(s)	111, 246, 390, 404, 2447
BciT130I	CCWGG	2 cut(s)	809, 1116
BciVI	GTATCC	1 cut(s)	1850
BcnI	CCSGG	4 cut(s)	280, 484, 726, 1872
BcoDI	GTCTC	5 cut(s)	280, 365, 726, 838, 1231
BfaI	CTAG	8 cut(s)	911, 951, 1517, 1583, 1716, 2088, 2232, 2625
BfmI	CTRYAG	6 cut(s)	194, 1770, 2040, 2154, 2244, 2600
BfoI	RGCGCY	1 cut(s)	242
BfuAI	ACCTGC	1 cut(s)	1714
BfuI	GTATCC	1 cut(s)	1850
BglI	GCCNNNNNGGC	1 cut(s)	233
BlnI	CCTAGG	1 cut(s)	910
BmcAI	AGTACT	1 cut(s)	2505
Bme1390I	CCNGG	6 cut(s)	280, 484, 726, 809, 1116, 1872
Bme18I	GGWCC	5 cut(s)	43, 722, 1069, 1253, 1376
BmgBI	CACGTC	1 cut(s)	2015
BmgT120I	GGNCC	7 cut(s)	43, 722, 1069, 1253, 1376, 1481, 2460
BmrFI	CCNGG	6 cut(s)	280, 484, 726, 809, 1116, 1872
BmrI	ACTGGG	2 cut(s)	1565, 1838
BmuI	ACTGGG	2 cut(s)	1565, 1838
BplI	GAGNNNNNCTC	2 cut(s)	18, 50
BpmI	CTGGAG	5 cut(s)	169, 1012, 2078, 2241, 2520
Bpu10I	CCTNAGC	1 cut(s)	389
Bpu14I	TTCGAA	1 cut(s)	164
BpuEI	CTTGAG	2 cut(s)	1314, 2584
BpuMI	CCSGG	4 cut(s)	280, 484, 726, 1872
Bsa29I	ATCGAT	1 cut(s)	2366
BsaBI	GATNNNNATC	1 cut(s)	802
BsaHI	GRCGYC	3 cut(s)	239, 257, 273
BsaI	GGTCTC	1 cut(s)	838
BsaJI	CCNNGG	6 cut(s)	228, 348, 483, 807, 910, 1131
BsaXI	ACNNNNNCTCC	6 cut(s)	242, 272, 1086, 1116, 1620, 1650
Bsc4I	CCNNNNNNNGG	5 cut(s)	349, 1136, 1877, 2101, 2313
Bse118I	RCCGGY	1 cut(s)	883
Bse1I	ACTGG	6 cut(s)	1029, 1285, 1560, 1653, 1844, 2415
Bse3DI	GCAATG	5 cut(s)	645, 1496, 1719, 2268, 2490
Bse8I	GATNNNNATC	1 cut(s)	802
BseBI	CCWGG	2 cut(s)	809, 1116
BseCI	ATCGAT	1 cut(s)	2366
BseDI	CCNNGG	6 cut(s)	228, 348, 483, 807, 910, 1131
BseJI	GATNNNNATC	1 cut(s)	802
BseLI	CCNNNNNNNGG	5 cut(s)	349, 1136, 1877, 2101, 2313
BseMI	GCAATG	5 cut(s)	645, 1496, 1719, 2268, 2490
BseMII	CTCAG	6 cut(s)	380, 1026, 1242, 1250, 1419, 2064
BseNI	ACTGG	6 cut(s)	1029, 1285, 1560, 1653, 1844, 2415
BseRI	GAGGAG	5 cut(s)	266, 484, 487, 554, 1641
BseSI	GKGCMC	1 cut(s)	1555
BseYI	CCCAGC	1 cut(s)	1478
Bsh1236I	CGCG	2 cut(s)	307, 464
Bsh1285I	CGRYCG	2 cut(s)	88, 337
BshNI	GGYRCC	1 cut(s)	238
BshVI	ATCGAT	1 cut(s)	2366
BsiEI	CGRYCG	2 cut(s)	88, 337
BsiHKAI	GWGCWC	4 cut(s)	675, 909, 1555, 2570
BsiSI	CCGG	9 cut(s)	41, 154, 279, 395, 438, 483, 726, 884, 1871
BslFI	GGGAC	4 cut(s)	735, 1055, 2144, 2349
BslI	CCNNNNNNNGG	5 cut(s)	349, 1136, 1877, 2101, 2313
BsmAI	GTCTC	5 cut(s)	280, 365, 726, 838, 1231
BsmBI	CGTCTC	3 cut(s)	280, 365, 1231
BsmFI	GGGAC	4 cut(s)	735, 1055, 2144, 2349
BsmI	GAATGC	2 cut(s)	160, 628
Bso31I	GGTCTC	1 cut(s)	838
Bsp119I	TTCGAA	1 cut(s)	164
Bsp1286I	GDGCHC	4 cut(s)	675, 909, 1555, 2570
Bsp143I	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
Bsp68I	TCGCGA	1 cut(s)	307
BspCNI	CTCAG	6 cut(s)	381, 1025, 1241, 1251, 1418, 2065
BspDI	ATCGAT	1 cut(s)	2366
BspFNI	CGCG	2 cut(s)	307, 464
BspHI	TCATGA	1 cut(s)	1936
BspMAI	CTGCAG	2 cut(s)	198, 2248
BspMI	ACCTGC	1 cut(s)	1714
BspPI	GGATC	6 cut(s)	613, 664, 778, 798, 811, 947
BspQI	GCTCTTC	1 cut(s)	2328
BspT104I	TTCGAA	1 cut(s)	164
BspT107I	GGYRCC	1 cut(s)	238
BspTNI	GGTCTC	1 cut(s)	838
BsrBI	CCGCTC	1 cut(s)	413
BsrDI	GCAATG	5 cut(s)	645, 1496, 1719, 2268, 2490
BsrFI	RCCGGY	1 cut(s)	883
BsrI	ACTGG	6 cut(s)	1029, 1285, 1560, 1653, 1844, 2415
BssAI	RCCGGY	1 cut(s)	883
BssECI	CCNNGG	6 cut(s)	228, 348, 483, 807, 910, 1131
BssMI	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
BssNAI	GTATAC	1 cut(s)	1387
BssNI	GRCGYC	3 cut(s)	239, 257, 273
BssT1I	CCWWGG	1 cut(s)	910
Bst1107I	GTATAC	1 cut(s)	1387
Bst2UI	CCWGG	2 cut(s)	809, 1116
Bst4CI	ACNGT	4 cut(s)	1039, 1225, 2083, 2116
Bst6I	CTCTTC	5 cut(s)	548, 843, 1135, 2133, 2328
BstACI	GRCGYC	3 cut(s)	239, 257, 273
BstAPI	GCANNNNNTGC	1 cut(s)	1951
BstBI	TTCGAA	1 cut(s)	164
BstC8I	GCNNGC	5 cut(s)	194, 198, 1359, 1947, 2060
BstDEI	CTNAG	6 cut(s)	389, 1012, 1228, 1259, 1405, 2073
BstDSI	CCRYGG	3 cut(s)	228, 348, 1131
BstFNI	CGCG	2 cut(s)	307, 464
BstH2I	RGCGCY	1 cut(s)	242
BstHHI	GCGC	1 cut(s)	241
BstKTI	GATC	6 cut(s)	608, 659, 773, 806, 942, 1663
BstMAI	GTCTC	5 cut(s)	280, 365, 726, 838, 1231
BstMBI	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
BstMCI	CGRYCG	2 cut(s)	88, 337
BstNI	CCWGG	2 cut(s)	809, 1116
BstNSI	RCATGY	2 cut(s)	364, 1949
BstSCI	CCNGG	6 cut(s)	278, 482, 724, 807, 1114, 1870
BstSFI	CTRYAG	6 cut(s)	194, 1770, 2040, 2154, 2244, 2600
BstSLI	GKGCMC	1 cut(s)	1555
BstUI	CGCG	2 cut(s)	307, 464
BstX2I	RGATCY	1 cut(s)	803
BstXI	CCANNNNNNTGG	1 cut(s)	1828
BstYI	RGATCY	1 cut(s)	803
BstZ17I	GTATAC	1 cut(s)	1387
Bsu15I	ATCGAT	1 cut(s)	2366
BsuI	GTATCC	1 cut(s)	1850
BsuTUI	ATCGAT	1 cut(s)	2366
BtgI	CCRYGG	3 cut(s)	228, 348, 1131
BtgZI	GCGATG	1 cut(s)	125
BtrI	CACGTC	1 cut(s)	2015
BtsI	GCAGTG	1 cut(s)	1920
BtsIMutI	CAGTG	7 cut(s)	974, 1035, 1036, 1553, 1692, 1920, 2408
BtuMI	TCGCGA	1 cut(s)	307
BveI	ACCTGC	1 cut(s)	1714
Cac8I	GCNNGC	5 cut(s)	194, 198, 1359, 1947, 2060
CciI	TCATGA	1 cut(s)	1936
CfoI	GCGC	1 cut(s)	241
Cfr10I	RCCGGY	1 cut(s)	883
Cfr13I	GGNCC	7 cut(s)	43, 722, 1069, 1253, 1376, 1481, 2460
ClaI	ATCGAT	1 cut(s)	2366
CseI	GACGC	1 cut(s)	246
Csp6I	GTAC	4 cut(s)	1040, 1429, 1779, 2504
CviQI	GTAC	4 cut(s)	1040, 1429, 1779, 2504
DdeI	CTNAG	6 cut(s)	389, 1012, 1228, 1259, 1405, 2073
DinI	GGCGCC	1 cut(s)	240
DpnI	GATC	6 cut(s)	607, 658, 772, 805, 941, 1662
DpnII	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
DraIII	CACNNNGTG	1 cut(s)	349
DrdI	GACNNNNNNGTC	1 cut(s)	338
DseDI	GACNNNNNNGTC	1 cut(s)	338
EaeI	YGGCCR	2 cut(s)	435, 1650
Eam1104I	CTCTTC	5 cut(s)	548, 843, 1135, 2133, 2328
EarI	CTCTTC	5 cut(s)	548, 843, 1135, 2133, 2328
Ecl136II	GAGCTC	1 cut(s)	2568
Eco130I	CCWWGG	1 cut(s)	910
Eco24I	GRGCYC	1 cut(s)	2570
Eco31I	GGTCTC	1 cut(s)	838
Eco47I	GGWCC	5 cut(s)	43, 722, 1069, 1253, 1376
Eco53kI	GAGCTC	1 cut(s)	2568
Eco57I	CTGAAG	2 cut(s)	1716, 2309
EcoICRI	GAGCTC	1 cut(s)	2568
EcoO109I	RGGNCCY	2 cut(s)	722, 1069
EcoRI	GAATTC	2 cut(s)	639, 784
EcoRII	CCWGG	2 cut(s)	807, 1114
EcoT14I	CCWWGG	1 cut(s)	910
EcoT38I	GRGCYC	1 cut(s)	2570
EgeI	GGCGCC	1 cut(s)	240
EheI	GGCGCC	1 cut(s)	240
ErhI	CCWWGG	1 cut(s)	910
Esp3I	CGTCTC	3 cut(s)	280, 365, 1231
FalI	AAGNNNNNCTT	2 cut(s)	1602, 1634
FaqI	GGGAC	4 cut(s)	735, 1055, 2144, 2349
FauI	CCCGC	1 cut(s)	2055
FblI	GTMKAC	2 cut(s)	84, 1386
FriOI	GRGCYC	1 cut(s)	2570
FspBI	CTAG	8 cut(s)	911, 951, 1517, 1583, 1716, 2088, 2232, 2625
GlaI	GCGC	1 cut(s)	240
GsaI	CCCAGC	1 cut(s)	1482
GsuI	CTGGAG	5 cut(s)	169, 1012, 2078, 2241, 2520
HaeII	RGCGCY	1 cut(s)	242
HapII	CCGG	9 cut(s)	41, 154, 279, 395, 438, 483, 726, 884, 1871
HgaI	GACGC	1 cut(s)	246
HhaI	GCGC	1 cut(s)	241
Hin1I	GRCGYC	3 cut(s)	239, 257, 273
Hin6I	GCGC	1 cut(s)	239
HinP1I	GCGC	1 cut(s)	239
HincII	GTYRAC	4 cut(s)	85, 358, 366, 447
HindII	GTYRAC	4 cut(s)	85, 358, 366, 447
HpaII	CCGG	9 cut(s)	41, 154, 279, 395, 438, 483, 726, 884, 1871
HphI	GGTGA	4 cut(s)	104, 562, 765, 2282
Hpy166II	GTNNAC	8 cut(s)	85, 343, 358, 366, 447, 1387, 1553, 2079
Hpy188III	TCNNGA	8 cut(s)	118, 248, 306, 923, 993, 1454, 1937, 2220
Hpy8I	GTNNAC	8 cut(s)	85, 343, 358, 366, 447, 1387, 1553, 2079
Hpy99I	CGWCG	5 cut(s)	132, 262, 275, 425, 2312
HpyCH4III	ACNGT	4 cut(s)	1039, 1225, 2083, 2116
HpyCH4IV	ACGT	5 cut(s)	273, 916, 2014, 2310, 2475
HpyF3I	CTNAG	6 cut(s)	389, 1012, 1228, 1259, 1405, 2073
HpySE526I	ACGT	5 cut(s)	273, 916, 2014, 2310, 2475
Hsp92I	GRCGYC	3 cut(s)	239, 257, 273
HspAI	GCGC	1 cut(s)	239
KasI	GGCGCC	1 cut(s)	238
KflI	GGGWCCC	1 cut(s)	722
Kzo9I	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
LguI	GCTCTTC	1 cut(s)	2328
LmnI	GCTCC	8 cut(s)	410, 678, 912, 1052, 1289, 1601, 1628, 2222
MaeI	CTAG	8 cut(s)	911, 951, 1517, 1583, 1716, 2088, 2232, 2625
MaeII	ACGT	5 cut(s)	273, 916, 2014, 2310, 2475
MaeIII	GTNAC	6 cut(s)	493, 972, 1031, 1846, 2083, 2581
MalI	GATC	6 cut(s)	607, 658, 772, 805, 941, 1662
MbiI	CCGCTC	1 cut(s)	413
MboI	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
MfeI	CAATTG	2 cut(s)	1794, 1998
MflI	RGATCY	1 cut(s)	803
MhlI	GDGCHC	4 cut(s)	675, 909, 1555, 2570
MlsI	TGGCCA	1 cut(s)	1652
MluNI	TGGCCA	1 cut(s)	1652
Mly113I	GGCGCC	1 cut(s)	239
MmeI	TCCRAC	3 cut(s)	1990, 2076, 2149
Mox20I	TGGCCA	1 cut(s)	1652
MroXI	GAANNNNTTC	3 cut(s)	1194, 1752, 1932
MscI	TGGCCA	1 cut(s)	1652
MseI	TTAA	6 cut(s)	384, 843, 1206, 1811, 1862, 2238
MslI	CAYNNNNRTG	1 cut(s)	1394
Msp20I	TGGCCA	1 cut(s)	1652
MspA1I	CMGCKG	2 cut(s)	189, 1232
MspI	CCGG	9 cut(s)	41, 154, 279, 395, 438, 483, 726, 884, 1871
MspR9I	CCNGG	6 cut(s)	280, 484, 726, 809, 1116, 1872
MunI	CAATTG	2 cut(s)	1794, 1998
Mva1269I	GAATGC	2 cut(s)	160, 628
MvaI	CCWGG	2 cut(s)	809, 1116
MvnI	CGCG	2 cut(s)	307, 464
NarI	GGCGCC	1 cut(s)	239
NciI	CCSGG	4 cut(s)	280, 484, 726, 1872
NdeII	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
NmeAIII	GCCGAG	1 cut(s)	451
NmuCI	GTSAC	4 cut(s)	493, 972, 1031, 1846
NruI	TCGCGA	1 cut(s)	307
NspI	RCATGY	2 cut(s)	364, 1949
NspV	TTCGAA	1 cut(s)	164
PaeI	GCATGC	1 cut(s)	1949
PagI	TCATGA	1 cut(s)	1936
PciI	ACATGT	1 cut(s)	360
PciSI	GCTCTTC	1 cut(s)	2328
PcsI	WCGNNNNNNNCGW	1 cut(s)	44
PctI	GAATGC	2 cut(s)	160, 628
PdmI	GAANNNNTTC	3 cut(s)	1194, 1752, 1932
PfeI	GAWTC	7 cut(s)	244, 459, 1022, 1097, 1190, 1364, 1493
PflFI	GACNNNGTC	1 cut(s)	338
PflMI	CCANNNNNTGG	1 cut(s)	349
PfoI	TCCNGGA	1 cut(s)	278
PluTI	GGCGCC	1 cut(s)	242
PpuMI	RGGWCCY	2 cut(s)	722, 1069
PscI	ACATGT	1 cut(s)	360
Psp124BI	GAGCTC	1 cut(s)	2570
Psp5II	RGGWCCY	2 cut(s)	722, 1069
Psp6I	CCWGG	2 cut(s)	807, 1114
PspFI	CCCAGC	1 cut(s)	1478
PspGI	CCWGG	2 cut(s)	807, 1114
PspPI	GGNCC	7 cut(s)	43, 722, 1069, 1253, 1376, 1481, 2460
PspPPI	RGGWCCY	2 cut(s)	722, 1069
PstI	CTGCAG	2 cut(s)	198, 2248
PsuI	RGATCY	1 cut(s)	803
PsyI	GACNNNGTC	1 cut(s)	338
PvuII	CAGCTG	2 cut(s)	189, 1232
RruI	TCGCGA	1 cut(s)	307
RsaI	GTAC	4 cut(s)	1041, 1430, 1780, 2505
RsaNI	GTAC	4 cut(s)	1040, 1429, 1779, 2504
RseI	CAYNNNNRTG	1 cut(s)	1394
SacI	GAGCTC	1 cut(s)	2570
SalI	GTCGAC	1 cut(s)	83
SapI	GCTCTTC	1 cut(s)	2328
SaqAI	TTAA	6 cut(s)	384, 843, 1206, 1811, 1862, 2238
Sau3AI	GATC	6 cut(s)	605, 656, 770, 803, 939, 1660
Sau96I	GGNCC	7 cut(s)	43, 722, 1069, 1253, 1376, 1481, 2460
SbfI	CCTGCAGG	1 cut(s)	198
ScaI	AGTACT	1 cut(s)	2505
ScrFI	CCNGG	6 cut(s)	280, 484, 726, 809, 1116, 1872
SdaI	CCTGCAGG	1 cut(s)	198
SduI	GDGCHC	4 cut(s)	675, 909, 1555, 2570
SfcI	CTRYAG	6 cut(s)	194, 1770, 2040, 2154, 2244, 2600
SfoI	GGCGCC	1 cut(s)	240
SfuI	TTCGAA	1 cut(s)	164
SinI	GGWCC	5 cut(s)	43, 722, 1069, 1253, 1376
SmiMI	CAYNNNNRTG	1 cut(s)	1394
SmlI	CTYRAG	2 cut(s)	1329, 2563
SmoI	CTYRAG	2 cut(s)	1329, 2563
SphI	GCATGC	1 cut(s)	1949
Sse8387I	CCTGCAGG	1 cut(s)	198
SspDI	GGCGCC	1 cut(s)	238
SspMI	CTAG	8 cut(s)	911, 951, 1517, 1583, 1716, 2088, 2232, 2625
SstI	GAGCTC	1 cut(s)	2570
StyD4I	CCNGG	6 cut(s)	278, 482, 724, 807, 1114, 1870
StyI	CCWWGG	1 cut(s)	910
TaaI	ACNGT	4 cut(s)	1039, 1225, 2083, 2116
TaiI	ACGT	5 cut(s)	276, 919, 2017, 2313, 2478
TaqI	TCGA	5 cut(s)	84, 164, 270, 2366, 2570
TaqII	GACCGA	2 cut(s)	351, 383
TatI	WGTACW	3 cut(s)	1039, 1778, 2503
TfiI	GAWTC	7 cut(s)	244, 459, 1022, 1097, 1190, 1364, 1493
Tru1I	TTAA	6 cut(s)	384, 843, 1206, 1811, 1862, 2238
Tru9I	TTAA	6 cut(s)	384, 843, 1206, 1811, 1862, 2238
TscAI	CASTG	7 cut(s)	981, 1036, 1042, 1560, 1699, 1920, 2415
TseFI	GTSAC	4 cut(s)	493, 972, 1031, 1846
Tsp45I	GTSAC	4 cut(s)	493, 972, 1031, 1846
TspDTI	ATGAA	7 cut(s)	20, 797, 974, 1353, 1485, 1925, 2597
TspGWI	ACGGA	3 cut(s)	337, 464, 512
TspRI	CASTG	7 cut(s)	981, 1036, 1042, 1560, 1699, 1920, 2415
Tth111I	GACNNNGTC	1 cut(s)	338
Van91I	CCANNNNNTGG	1 cut(s)	349
VneI	GTGCAC	1 cut(s)	1551
VpaK11BI	GGWCC	5 cut(s)	43, 722, 1069, 1253, 1376
XapI	RAATTY	5 cut(s)	639, 784, 1749, 2183, 2449
XceI	RCATGY	2 cut(s)	364, 1949
XmaJI	CCTAGG	1 cut(s)	910
XmiI	GTMKAC	2 cut(s)	84, 1386
XmnI	GAANNNNTTC	3 cut(s)	1194, 1752, 1932
XspI	CTAG	8 cut(s)	911, 951, 1517, 1583, 1716, 2088, 2232, 2625
ZraI	GACGTC	1 cut(s)	274
ZrmI	AGTACT	1 cut(s)	2505

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.