FvH4_2g31380
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009262
Physical Location & Seq
Reverse (-)
24060990 .. 24064225
3236 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31380.t1

Sequence Viewer

Length: 1500 bp
ATGGCGCGTACGAGAGTCGCAGCGACGCCGCCGTCGACGGCCGGCGAGAAGTCAGAAACACGGCGCCGTTTAAGCATGGAGAGGTCGGCGCTGTTCGCCAGGAGAGAGGCGGCGAAGGTTTTGAAGCAAATTCTCCAAGGCGACTCCCGGCGGCGGTCCGTCGGCTCTATCAAGTCGCTCGTTTACAGTCCGTCGGTGAGGAACAAGAAGGGGACCTTTGCTCTGGTCTGCGAAACCCTAAAGCATCTCAAGATAATCAAACAGGTGTTGAATGCTGCAGACGTTCTGAATAGCAAGTGGAGGAAGCAAGATGAGTTGGTTTACATTGTCACCTATGATATTCTTTTCGGCAAGGTGACTTCGTCGACTGGTGATGCAGAGAGGTTTCTCTTGCTTCGGAAAGGGGCTCTGCAGGCAGCTCTAGATCGCCTTTTAGTGAAGAAAAAAGTGAAGAGCGTTGAAGAGTTGATAGCTCTCGATCAGACTCCTGAGATTTCAAAACCTCGGTATGTTCGTGTTAATACTCTGAAAATCGATGTCAATACAGCCTTGTTTGAATTACAGAAACAATACAAGGTTCAAAAGGATGATCTGCTTCCTGACTTGCTGATTCTTCCACCCAGCAGTGACTTGCATGACCATCCTCTAGTTGTCAACGGAAGTGTCTTCATGCAAGGAAAGGCAAGCTCTATGGTTGCAACAGCCCTTGCTCCAAAACCAGGGTGGGAGGTTCTTGATGCATGCTCAGCTCCAGGAAATAAAACAGTCCACCTAGCAGCTCTTATGGGAGATGAAGGAAAAGTCATAGCATGTGAGCTGAATGAAGAGAGGGTTAAACGACTAAAAGAGACGATCAGACTCTCGGGTGCCTCTAATATTAAGGTTCTGCACGGAGATTTCTTACACTTGAACCCTGAGGATCCTGCATATTCCAAGGTTCAGGCCATTCTATTGGATCCTTCCTGCTCTGGATCCGGGACAGCTTCTGCAAGATTAGATCACTTGCTCCCTTCTTCTGCTCCTGGTCACACTGCCGACTTCACAGAGACAGAAAGACTGAACAAGCTTGCAGCGTTTCAGGAAAAAGCTCTGGCTCATGCGTTGTCTTTTCCAGAAGTGGAAAGAATTGTTTACAGCACATGCTCTGTCAACCAAATTGAAAATGAGGATGTCGTCAATTCTGTTCTACCTCTTGCTGAATCTTATGGTTTTGAACTTGAAACTCTGTTTCCCAAATGGCAACGCCGTGGTCTTCCGGTTTTCAAGGGTGCCGAACGCCTGCTTCGAACTGATCCTGTTGAGGACAAAGAAGGCTTCTTCATTGCTTTGTTTGTCAGAAAACGCTCTGATCAATCAGAGGAACCTAATTTGACAAATGAAGATAACACTCGCAAACATCGACATGCAAGTAAGAGAATTGTTGTACCACCCACTACCAATTTGTTTAAAATCTGGGCACATGCTATGGTGAATAGGCCAAACTGTAGTTGTTGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltr_RsmB-F

Protein Analysis

500

Amino Acids

55.5

Weight (kDa)

9.29

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NSUN5_N PF21153 72 - 157 4.4e-16 NOL1/NOP2/Sun domain family member 5, N-terminal domain
NSUN5_fdxn-like PF21148 191 - 225 1.9e-06 NOL1/NOP2/Sun domain family member 5, ferredoxin-like domain
Ubie_methyltran PF01209 232 - 310 3.8e-06 ubiE/COQ5 methyltransferase family
Methyltr_RsmB-F PF01189 233 - 444 7.2e-27 16S rRNA methyltransferase RsmB/F
Methyltransf_31 PF13847 239 - 304 7.3e-06 Methyltransferase domain
Methyltransf_25 PF13649 244 - 313 4.1e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 31
AccB1I GGYRCC 3 cut(s) 63, 866, 1268
AccI GTMKAC 2 cut(s) 35, 365
AccII CGCG 1 cut(s) 7
AciI CCGC 5 cut(s) 29, 110, 151, 154, 1494
AclWI GGATC 7 cut(s) 914, 927, 950, 963, 966, 979, 1286
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 129
AcyI GRCGYC 2 cut(s) 26, 64
AfaI GTAC 2 cut(s) 10, 1425
AfiI CCNNNNNNNGG 2 cut(s) 153, 719
AjnI CCWGG 4 cut(s) 98, 718, 751, 1021
AluBI AGCT 9 cut(s) 419, 473, 687, 749, 779, 817, 983, 1066, 1088
AluI AGCT 9 cut(s) 419, 473, 687, 749, 779, 817, 983, 1066, 1088
Alw26I GTCTC 2 cut(s) 842, 1040
AlwI GGATC 7 cut(s) 914, 927, 950, 963, 966, 979, 1286
AlwNI CAGNNNCTG 1 cut(s) 986
Ama87I CYCGRG 1 cut(s) 862
AoxI GGCC 3 cut(s) 39, 942, 1475
ApeKI GCWGC 5 cut(s) 20, 275, 416, 776, 1070
ApoI RAATTY 1 cut(s) 129
AspLEI GCGC 3 cut(s) 7, 66, 91
AspS9I GGNCC 2 cut(s) 156, 213
AsuC2I CCSGG 2 cut(s) 148, 976
AsuHPI GGTGA 5 cut(s) 208, 322, 367, 383, 1480
AsuII TTCGAA 1 cut(s) 1285
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 2 cut(s) 156, 213
AxyI CCTNAGG 1 cut(s) 915
BaeGI GKGCMC 1 cut(s) 1459
BamHI GGATCC 3 cut(s) 919, 955, 971
BanI GGYRCC 3 cut(s) 63, 866, 1268
BanII GRGCYC 1 cut(s) 409
BbsI GAAGAC 2 cut(s) 658, 1244
BbvI GCAGC 5 cut(s) 32, 262, 428, 788, 1082
BccI CCATC 1 cut(s) 648
BceAI ACGGC 5 cut(s) 16, 51, 54, 77, 1230
BciT130I CCWGG 4 cut(s) 100, 720, 753, 1023
BclI TGATCA 1 cut(s) 1348
BcnI CCSGG 2 cut(s) 148, 976
BcoDI GTCTC 2 cut(s) 842, 1040
BfaI CTAG 3 cut(s) 422, 647, 773
BfmI CTRYAG 3 cut(s) 276, 410, 1483
BfoI RGCGCY 2 cut(s) 67, 92
BisI GCNGC 8 cut(s) 21, 29, 111, 152, 276, 417, 777, 1071
BlpI GCTNAGC 1 cut(s) 745
BlsI GCNGC 8 cut(s) 22, 30, 112, 153, 277, 418, 778, 1072
Bme1390I CCNGG 6 cut(s) 100, 148, 720, 753, 976, 1023
Bme18I GGWCC 2 cut(s) 156, 213
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 2 cut(s) 156, 213
BmiI GGNNCC 8 cut(s) 65, 214, 868, 921, 957, 973, 1270, 1362
BmrFI CCNGG 6 cut(s) 100, 148, 720, 753, 976, 1023
BmsI GCATC 3 cut(s) 253, 364, 727
BpiI GAAGAC 2 cut(s) 658, 1244
BpmI CTGGAG 1 cut(s) 735
Bpu1102I GCTNAGC 1 cut(s) 745
Bpu14I TTCGAA 1 cut(s) 1285
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 2 cut(s) 148, 976
Bsa29I ATCGAT 1 cut(s) 534
BsaHI GRCGYC 2 cut(s) 26, 64
BsaJI CCNNGG 5 cut(s) 136, 503, 719, 933, 1246
BsaWI WCCGGW 1 cut(s) 1255
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 719
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 373
Bse21I CCTNAGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 1320
BseBI CCWGG 4 cut(s) 100, 720, 753, 1023
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 5 cut(s) 136, 503, 719, 933, 1246
BseGI GGATG 3 cut(s) 592, 640, 1174
BseLI CCNNNNNNNGG 2 cut(s) 153, 719
BseMI GCAATG 1 cut(s) 1320
BseMII CTCAG 3 cut(s) 480, 759, 906
BseNI ACTGG 1 cut(s) 373
BseSI GKGCMC 1 cut(s) 1459
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 5 cut(s) 32, 262, 428, 788, 1082
BseYI CCCAGC 1 cut(s) 620
BsgI GTGCAG 1 cut(s) 872
Bsh1236I CGCG 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 3 cut(s) 41, 944, 1477
BshNI GGYRCC 3 cut(s) 63, 866, 1268
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 862
BsiSI CCGG 4 cut(s) 42, 148, 975, 1256
BsiWI CGTACG 1 cut(s) 8
BslFI GGGAC 2 cut(s) 226, 991
BslI CCNNNNNNNGG 2 cut(s) 153, 719
BsmAI GTCTC 2 cut(s) 842, 1040
BsmBI CGTCTC 1 cut(s) 842
BsmFI GGGAC 2 cut(s) 226, 991
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 3 cut(s) 41, 944, 1477
BsoBI CYCGRG 1 cut(s) 862
Bsp119I TTCGAA 1 cut(s) 1285
Bsp1286I GDGCHC 2 cut(s) 409, 1459
Bsp1720I GCTNAGC 1 cut(s) 745
BspACI CCGC 5 cut(s) 29, 110, 151, 154, 1494
BspANI GGCC 3 cut(s) 41, 944, 1477
BspCNI CTCAG 3 cut(s) 481, 758, 907
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 8 cut(s) 65, 214, 868, 921, 957, 973, 1270, 1362
BspMAI CTGCAG 2 cut(s) 280, 414
BspPI GGATC 7 cut(s) 914, 927, 950, 963, 966, 979, 1286
BspQI GCTCTTC 1 cut(s) 446
BspT104I TTCGAA 1 cut(s) 1285
BspT107I GGYRCC 3 cut(s) 63, 866, 1268
BsrDI GCAATG 1 cut(s) 1320
BsrFI RCCGGY 1 cut(s) 41
BsrI ACTGG 1 cut(s) 373
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 5 cut(s) 136, 503, 719, 933, 1246
BssNI GRCGYC 2 cut(s) 26, 64
BssT1I CCWWGG 2 cut(s) 136, 933
Bst2UI CCWGG 4 cut(s) 100, 720, 753, 1023
Bst4CI ACNGT 3 cut(s) 188, 766, 1484
Bst6I CTCTTC 3 cut(s) 446, 456, 819
BstACI GRCGYC 2 cut(s) 26, 64
BstBI TTCGAA 1 cut(s) 1285
BstC8I GCNNGC 6 cut(s) 43, 414, 685, 742, 1068, 1280
BstDEI CTNAG 3 cut(s) 489, 745, 915
BstDSI CCRYGG 1 cut(s) 1246
BstF5I GGATG 3 cut(s) 592, 640, 1174
BstFNI CGCG 1 cut(s) 7
BstH2I RGCGCY 2 cut(s) 67, 92
BstHHI GCGC 3 cut(s) 7, 66, 91
BstMAI GTCTC 2 cut(s) 842, 1040
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 4 cut(s) 72, 95, 413, 746
BstNI CCWGG 4 cut(s) 100, 720, 753, 1023
BstNSI RCATGY 5 cut(s) 744, 813, 1143, 1406, 1463
BstSCI CCNGG 6 cut(s) 98, 146, 718, 751, 974, 1021
BstSFI CTRYAG 3 cut(s) 276, 410, 1483
BstSLI GKGCMC 1 cut(s) 1459
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 5 cut(s) 32, 262, 428, 788, 1082
BstV2I GAAGAC 2 cut(s) 658, 1244
BstX2I RGATCY 3 cut(s) 919, 955, 971
BstXI CCANNNNNNTGG 1 cut(s) 952
BstYI RGATCY 3 cut(s) 919, 955, 971
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 534
Bsu36I CCTNAGG 1 cut(s) 915
BsuRI GGCC 3 cut(s) 41, 944, 1477
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 1246
BtsCI GGATG 3 cut(s) 592, 640, 1174
BtsI GCAGTG 2 cut(s) 631, 1029
BtsIMutI CAGTG 2 cut(s) 631, 1029
Cac8I GCNNGC 6 cut(s) 43, 414, 685, 742, 1068, 1280
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 3 cut(s) 7, 66, 91
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 156, 213
ClaI ATCGAT 1 cut(s) 534
CpoI CGGWCCG 1 cut(s) 156
CseI GACGC 1 cut(s) 34
Csp6I GTAC 2 cut(s) 9, 1424
CspI CGGWCCG 1 cut(s) 156
CviAII CATG 9 cut(s) 76, 635, 670, 741, 810, 1097, 1140, 1403, 1460
CviQI GTAC 2 cut(s) 9, 1424
DdeI CTNAG 3 cut(s) 489, 745, 915
DinI GGCGCC 1 cut(s) 65
DraI TTTAAA 1 cut(s) 1447
DrdI GACNNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 31
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 446, 456, 819
EarI CTCTTC 3 cut(s) 446, 456, 819
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 2 cut(s) 136, 933
Eco24I GRGCYC 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 156, 213
Eco52I CGGCCG 1 cut(s) 39
Eco81I CCTNAGG 1 cut(s) 915
Eco88I CYCGRG 1 cut(s) 862
EcoO109I RGGNCCY 1 cut(s) 213
EcoRII CCWGG 4 cut(s) 98, 718, 751, 1021
EcoT14I CCWWGG 2 cut(s) 136, 933
EcoT22I ATGCAT 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 409
EgeI GGCGCC 1 cut(s) 65
EheI GGCGCC 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 136, 933
Esp3I CGTCTC 1 cut(s) 842
FaeI CATG 9 cut(s) 79, 638, 673, 744, 813, 1100, 1143, 1406, 1463
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 2 cut(s) 226, 991
FatI CATG 9 cut(s) 75, 634, 669, 740, 809, 1096, 1139, 1402, 1459
FbaI TGATCA 1 cut(s) 1348
FblI GTMKAC 2 cut(s) 35, 365
Fnu4HI GCNGC 8 cut(s) 21, 29, 111, 152, 276, 417, 777, 1071
FokI GGATG 3 cut(s) 599, 627, 1181
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 8 cut(s) 21, 29, 111, 152, 276, 417, 777, 1071
FspBI CTAG 3 cut(s) 422, 647, 773
GlaI GCGC 3 cut(s) 6, 65, 90
GluI GCNGC 8 cut(s) 21, 29, 111, 152, 276, 417, 777, 1071
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 1 cut(s) 735
HaeII RGCGCY 2 cut(s) 67, 92
HaeIII GGCC 3 cut(s) 41, 944, 1477
HapII CCGG 4 cut(s) 42, 148, 975, 1256
HgaI GACGC 1 cut(s) 34
HhaI GCGC 3 cut(s) 7, 66, 91
Hin1I GRCGYC 2 cut(s) 26, 64
Hin1II CATG 9 cut(s) 79, 638, 673, 744, 813, 1100, 1143, 1406, 1463
Hin6I GCGC 3 cut(s) 5, 64, 89
HinP1I GCGC 3 cut(s) 5, 64, 89
HincII GTYRAC 4 cut(s) 36, 366, 655, 1150
HindII GTYRAC 4 cut(s) 36, 366, 655, 1150
HindIII AAGCTT 1 cut(s) 1064
HinfI GANTC 6 cut(s) 15, 143, 484, 610, 858, 1199
HpaII CCGG 4 cut(s) 42, 148, 975, 1256
HphI GGTGA 5 cut(s) 208, 322, 367, 383, 1480
Hpy166II GTNNAC 8 cut(s) 36, 184, 322, 366, 655, 769, 1132, 1150
Hpy188I TCNGA 9 cut(s) 55, 288, 399, 483, 528, 857, 1337, 1348, 1357
Hpy188III TCNNGA 9 cut(s) 250, 422, 476, 488, 599, 734, 969, 1079, 1112
Hpy8I GTNNAC 8 cut(s) 36, 184, 322, 366, 655, 769, 1132, 1150
Hpy99I CGWCG 6 cut(s) 28, 37, 40, 164, 196, 367
HpyAV CCTTC 6 cut(s) 109, 202, 788, 969, 1020, 1304
HpyCH4III ACNGT 3 cut(s) 188, 766, 1484
HpyCH4IV ACGT 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 95, 413, 746
HpyF3I CTNAG 3 cut(s) 489, 745, 915
HpySE526I ACGT 1 cut(s) 282
Hsp92I GRCGYC 2 cut(s) 26, 64
Hsp92II CATG 9 cut(s) 79, 638, 673, 744, 813, 1100, 1143, 1406, 1463
HspAI GCGC 3 cut(s) 5, 64, 89
KasI GGCGCC 1 cut(s) 63
KroI GCCGGC 1 cut(s) 41
KroNI GCCGGC 1 cut(s) 43
Ksp22I TGATCA 1 cut(s) 1348
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 4 cut(s) 715, 754, 1011, 1024
Lsp1109I GCAGC 5 cut(s) 32, 262, 428, 788, 1082
LweI GCATC 3 cut(s) 253, 364, 727
MaeI CTAG 3 cut(s) 422, 647, 773
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 4 cut(s) 328, 355, 626, 1025
MflI RGATCY 3 cut(s) 919, 955, 971
MhlI GDGCHC 2 cut(s) 409, 1459
MluCI AATT 8 cut(s) 129, 557, 1125, 1155, 1177, 1366, 1416, 1438
Mly113I GGCGCC 1 cut(s) 64
MlyI GAGTC 4 cut(s) 24, 137, 478, 852
Mph1103I ATGCAT 1 cut(s) 742
MroNI GCCGGC 1 cut(s) 41
MseI TTAA 5 cut(s) 71, 519, 834, 879, 1446
MslI CAYNNNNRTG 1 cut(s) 1401
MspI CCGG 4 cut(s) 42, 148, 975, 1256
MspR9I CCNGG 6 cut(s) 100, 148, 720, 753, 976, 1023
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 4 cut(s) 100, 720, 753, 1023
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 4 cut(s) 72, 95, 413, 746
NaeI GCCGGC 1 cut(s) 43
NarI GGCGCC 1 cut(s) 64
NciI CCSGG 2 cut(s) 148, 976
NgoMIV GCCGGC 1 cut(s) 41
NlaIII CATG 9 cut(s) 79, 638, 673, 744, 813, 1100, 1143, 1406, 1463
NlaIV GGNNCC 8 cut(s) 65, 214, 868, 921, 957, 973, 1270, 1362
NmuCI GTSAC 4 cut(s) 328, 355, 626, 1025
NsiI ATGCAT 1 cut(s) 742
NspI RCATGY 5 cut(s) 744, 813, 1143, 1406, 1463
NspV TTCGAA 1 cut(s) 1285
PaeI GCATGC 1 cut(s) 744
PciSI GCTCTTC 1 cut(s) 446
PcsI WCGNNNNNNNCGW 4 cut(s) 32, 511, 1282, 1396
PctI GAATGC 1 cut(s) 277
PdiI GCCGGC 1 cut(s) 43
PfeI GAWTC 2 cut(s) 610, 1199
Pfl23II CGTACG 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 361
PfoI TCCNGGA 2 cut(s) 751, 974
PkrI GCNGC 8 cut(s) 22, 30, 112, 153, 277, 418, 778, 1072
PleI GAGTC 4 cut(s) 23, 137, 478, 852
PluTI GGCGCC 1 cut(s) 67
PpsI GAGTC 4 cut(s) 23, 137, 478, 852
PpuMI RGGWCCY 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 213
Psp6I CCWGG 4 cut(s) 98, 718, 751, 1021
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 4 cut(s) 98, 718, 751, 1021
PspLI CGTACG 1 cut(s) 8
PspN4I GGNNCC 8 cut(s) 65, 214, 868, 921, 957, 973, 1270, 1362
PspPI GGNCC 2 cut(s) 156, 213
PspPPI RGGWCCY 1 cut(s) 213
PstI CTGCAG 2 cut(s) 280, 414
PstNI CAGNNNCTG 1 cut(s) 986
PsuI RGATCY 3 cut(s) 919, 955, 971
PsyI GACNNNGTC 1 cut(s) 361
RsaI GTAC 2 cut(s) 10, 1425
RsaNI GTAC 2 cut(s) 9, 1424
RseI CAYNNNNRTG 1 cut(s) 1401
Rsr2I CGGWCCG 1 cut(s) 156
RsrII CGGWCCG 1 cut(s) 156
SalI GTCGAC 2 cut(s) 34, 364
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 5 cut(s) 71, 519, 834, 879, 1446
SatI GCNGC 8 cut(s) 21, 29, 111, 152, 276, 417, 777, 1071
Sau96I GGNCC 2 cut(s) 156, 213
SchI GAGTC 4 cut(s) 24, 137, 478, 852
ScrFI CCNGG 6 cut(s) 100, 148, 720, 753, 976, 1023
SduI GDGCHC 2 cut(s) 409, 1459
SfaNI GCATC 3 cut(s) 253, 364, 727
SfcI CTRYAG 3 cut(s) 276, 410, 1483
SfoI GGCGCC 1 cut(s) 65
SfuI TTCGAA 1 cut(s) 1285
SgrDI CGTCGACG 1 cut(s) 34
SinI GGWCC 2 cut(s) 156, 213
SmiMI CAYNNNNRTG 1 cut(s) 1401
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SphI GCATGC 1 cut(s) 744
Sse9I AATT 8 cut(s) 129, 557, 1125, 1155, 1177, 1366, 1416, 1438
SsiI CCGC 5 cut(s) 29, 110, 151, 154, 1494
SspDI GGCGCC 1 cut(s) 63
SspI AATATT 1 cut(s) 877
SspMI CTAG 3 cut(s) 422, 647, 773
StyD4I CCNGG 6 cut(s) 98, 146, 718, 751, 974, 1021
StyI CCWWGG 2 cut(s) 136, 933
TaaI ACNGT 3 cut(s) 188, 766, 1484
TaiI ACGT 1 cut(s) 285
TaqI TCGA 6 cut(s) 35, 365, 477, 534, 1285, 1399
TasI AATT 8 cut(s) 129, 557, 1125, 1155, 1177, 1366, 1416, 1438
TauI GCSGC 3 cut(s) 31, 113, 154
TfiI GAWTC 2 cut(s) 610, 1199
Tru1I TTAA 5 cut(s) 71, 519, 834, 879, 1446
Tru9I TTAA 5 cut(s) 71, 519, 834, 879, 1446
TscAI CASTG 2 cut(s) 631, 1036
TseFI GTSAC 4 cut(s) 328, 355, 626, 1025
TseI GCWGC 5 cut(s) 20, 275, 416, 776, 1070
Tsp45I GTSAC 4 cut(s) 328, 355, 626, 1025
TspDTI ATGAA 5 cut(s) 658, 807, 837, 1309, 1392
TspGWI ACGGA 4 cut(s) 148, 180, 672, 906
TspRI CASTG 2 cut(s) 631, 1036
Tth111I GACNNNGTC 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 156, 213
XapI RAATTY 1 cut(s) 129
XbaI TCTAGA 1 cut(s) 421
XceI RCATGY 5 cut(s) 744, 813, 1143, 1406, 1463
XcmI CCANNNNNNNNNTGG 1 cut(s) 720
XmiI GTMKAC 2 cut(s) 35, 365
XspI CTAG 3 cut(s) 422, 647, 773
Zsp2I ATGCAT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.