Rorug06G0389100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001449
Physical Location & Seq
Forward (+)
53353631 .. 53357948
4318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0389100.1

Sequence Viewer

Length: 1359 bp
ATGGATTCGGATAATTTGGAGGCCGCCTATGGGCTTCAGGTTGCTCTTGAAAATGGTGGTCATGGGCAACTTAGTGTTGGTCCAGACGGTGAAACTACTCTCACCGAGACTGCAGCACCGAATGGGAGAATTGAAAATGTGGTGAACTCGGATGATGGTGTGTCGTATAATTCATCTGCTGGAGAAGTCAAAGAGGAATCCAGTGTCAACTCTGCAAGCAATGGTTTGACTATTGCTAAGGAAAGGGGACCGAAAGTTTCTGTTCAGTCAAAGCAATCCAAAGTGCAGAAGGGTCAAGGTAAGAGTAAGATTGAGAAGCCCCCTAGTCCCAAGAGCGCCTTGCCAATTCGGATGAAGAAAAGCAAAGATGGAAATGATGCAGAGGCTACAGCTACTCTTTCCAATGGTTCAGCAGGTCCAAATTCACTCGCCAAGCAGCCTAACAAGAGTAGATCATTGAATGGCCAACAGCAGCAACCTAAACAATCTGAGGCACCATCGACTGAAGGCCTTGTGGAGGAGACAAATTTGAAGCCACTAAACAAAGGCTCCTATAATAAGGCTGATGGAGACTCCCAATCTTCTTTAAGTCCCACGGAAGGAGATATGAAAGCACCTAGGGTGGGTACACTGCCAAAGTATGGTTTCAGCTTTAAGTGTGATGAAAGAGCTGAGAAAAGAAGAGAGTTCTACACCAAACTTGAGGAAAAGATACATGCAAAGGAGATGGAGAAGAATAATTTGCAAGCCAAGTCTAAGGAAACTCTAGAAGCTGAGATTAAGATGCTCAGGAAGAAGCTGACTTTTAAAGCAACTCCAATGCCAAGCTTTTATCAGGAGCCTCCGCCTCCTAAGGTGGAGCTAAAGAAGTTACCAACAACAAGAGCCAAGTCTCCCAAGCTCGGCCGCAGGAAGAGCTTACCTCCAGCAGATTCTGAAGGAAACAGTAATACCAAGAGTCAATCTGGCAGGCTTAGTCTGGGTGAGAAACTTCCTCAAAACTCTGCCAAAGGACCTTCTCCTGTGCATCCAAAGAAGCCACAAAGAAAGTCTCTTCCTAGACTTCCATCTGAGAAGACCACTTTATCTAATGTGAAGAGTGTGCGGAAGGTTACTTCTAAGGCAACAAACGAGGAAAAGGCTGCCGCGTTGCCCAATGCAACAAGTGAAGCTGGACCTCACACCCAAGAGCAGGAGGGAGTTCCCAGAGCTGAGATAACTGAACCCTACACCCAAGAGCCGGAAGCAGTTCCCAGGGCTGCAATAAGTGAGGAGCAGTCCCATTCAGATGATGAGACAGTTAATGAAGAGGAACATCATCCCACCTTGGTTCAAGAGCCTATAGCGCTGGAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltr_RsmB-F

Protein Analysis

452

Amino Acids

48.86

Weight (kDa)

8.94

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 216 - 286 4.4e-27 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 641
AccII CGCG 1 cut(s) 1148
AciI CCGC 5 cut(s) 24, 845, 907, 1105, 1146
AcoI YGGCCR 2 cut(s) 463, 904
AcsI RAATTY 2 cut(s) 421, 526
AcuI CTGAAG 3 cut(s) 20, 525, 957
AfaI GTAC 1 cut(s) 628
AfeI AGCGCT 1 cut(s) 1347
AfiI CCNNNNNNNGG 8 cut(s) 30, 517, 599, 623, 641, 902, 1192, 1240
AgsI TTSAA 5 cut(s) 50, 134, 460, 532, 1334
AjnI CCWGG 1 cut(s) 1253
Alw26I GTCTC 6 cut(s) 101, 515, 564, 897, 1056, 1289
AlwNI CAGNNNCTG 1 cut(s) 935
Aor51HI AGCGCT 1 cut(s) 1347
AoxI GGCC 4 cut(s) 21, 463, 508, 904
ApeKI GCWGC 5 cut(s) 113, 436, 472, 1142, 1259
ApoI RAATTY 2 cut(s) 421, 526
Asp700I GAANNNNTTC 1 cut(s) 1248
AspA2I CCTAGG 1 cut(s) 617
AspLEI GCGC 2 cut(s) 338, 1348
AspS9I GGNCC 5 cut(s) 80, 248, 416, 1013, 1175
AsuHPI GGTGA 4 cut(s) 94, 101, 154, 995
AvaII GGWCC 5 cut(s) 80, 248, 416, 1013, 1175
AvrII CCTAGG 1 cut(s) 617
AxyI CCTNAGG 1 cut(s) 852
BalI TGGCCA 1 cut(s) 465
BanI GGYRCC 1 cut(s) 493
BbsI GAAGAC 1 cut(s) 1082
BbvI GCAGC 5 cut(s) 125, 448, 484, 1129, 1246
BccI CCATC 6 cut(s) 149, 362, 505, 560, 721, 1075
BciT130I CCWGG 1 cut(s) 1255
BcoDI GTCTC 6 cut(s) 101, 515, 564, 897, 1056, 1289
BfaI CTAG 4 cut(s) 324, 618, 767, 1059
BfmI CTRYAG 3 cut(s) 111, 387, 1341
BfoI RGCGCY 2 cut(s) 339, 1349
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 8 cut(s) 24, 114, 437, 473, 907, 1143, 1146, 1260
BlnI CCTAGG 1 cut(s) 617
BlsI GCNGC 8 cut(s) 25, 115, 438, 474, 908, 1144, 1147, 1261
Bme1390I CCNGG 1 cut(s) 1255
Bme18I GGWCC 5 cut(s) 80, 248, 416, 1013, 1175
BmgT120I GGNCC 5 cut(s) 80, 248, 416, 1013, 1175
BmiI GGNNCC 4 cut(s) 249, 495, 550, 840
BmrFI CCNGG 1 cut(s) 1255
BmsI GCATC 3 cut(s) 367, 774, 1036
BpiI GAAGAC 1 cut(s) 1082
BplI GAGNNNNNCTC 2 cut(s) 907, 939
BpmI CTGGAG 2 cut(s) 201, 909
Bpu10I CCTNAGC 2 cut(s) 237, 788
BpuEI CTTGAG 1 cut(s) 722
BsaJI CCNNGG 5 cut(s) 594, 617, 1253, 1254, 1326
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 8 cut(s) 30, 517, 599, 623, 641, 902, 1192, 1240
Bse1I ACTGG 1 cut(s) 201
Bse21I CCTNAGG 1 cut(s) 852
Bse3DI GCAATG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 1255
BseDI CCNNGG 5 cut(s) 594, 617, 1253, 1254, 1326
BseGI GGATG 4 cut(s) 157, 357, 1027, 1318
BseLI CCNNNNNNNGG 8 cut(s) 30, 517, 599, 623, 641, 902, 1192, 1240
BseMI GCAATG 1 cut(s) 226
BseMII CTCAG 6 cut(s) 480, 663, 765, 802, 1062, 1203
BseNI ACTGG 1 cut(s) 201
BseRI GAGGAG 2 cut(s) 533, 1286
BseX3I CGGCCG 1 cut(s) 904
BseXI GCAGC 5 cut(s) 125, 448, 484, 1129, 1246
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 1 cut(s) 1148
Bsh1285I CGRYCG 1 cut(s) 907
BshFI GGCC 4 cut(s) 23, 465, 510, 906
BshNI GGYRCC 1 cut(s) 493
BsiEI CGRYCG 1 cut(s) 907
BsiSI CCGG 1 cut(s) 1241
BslFI GGGAC 4 cut(s) 261, 312, 576, 1264
BslI CCNNNNNNNGG 8 cut(s) 30, 517, 599, 623, 641, 902, 1192, 1240
BsmAI GTCTC 6 cut(s) 101, 515, 564, 897, 1056, 1289
BsmFI GGGAC 4 cut(s) 261, 312, 576, 1264
BsnI GGCC 4 cut(s) 23, 465, 510, 906
Bsp143I GATC 1 cut(s) 452
BspACI CCGC 5 cut(s) 24, 845, 907, 1105, 1146
BspANI GGCC 4 cut(s) 23, 465, 510, 906
BspCNI CTCAG 6 cut(s) 481, 664, 766, 801, 1063, 1204
BspFNI CGCG 1 cut(s) 1148
BspLI GGNNCC 4 cut(s) 249, 495, 550, 840
BspMAI CTGCAG 1 cut(s) 115
BspMI ACCTGC 1 cut(s) 404
BspQI GCTCTTC 1 cut(s) 908
BspT107I GGYRCC 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 226
BsrI ACTGG 1 cut(s) 201
BssECI CCNNGG 5 cut(s) 594, 617, 1253, 1254, 1326
BssMI GATC 1 cut(s) 452
BssT1I CCWWGG 2 cut(s) 617, 1326
Bst2UI CCWGG 1 cut(s) 1255
Bst4CI ACNGT 3 cut(s) 89, 947, 1300
Bst6I CTCTTC 5 cut(s) 676, 908, 1059, 1091, 1302
BstC8I GCNNGC 3 cut(s) 217, 747, 971
BstDSI CCRYGG 1 cut(s) 594
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 4 cut(s) 157, 357, 1027, 1318
BstFNI CGCG 1 cut(s) 1148
BstH2I RGCGCY 2 cut(s) 339, 1349
BstHHI GCGC 2 cut(s) 338, 1348
BstKTI GATC 1 cut(s) 455
BstMAI GTCTC 6 cut(s) 101, 515, 564, 897, 1056, 1289
BstMBI GATC 1 cut(s) 452
BstMCI CGRYCG 1 cut(s) 907
BstMWI GCNNNNNNNGC 2 cut(s) 915, 1345
BstNI CCWGG 1 cut(s) 1255
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 1 cut(s) 1253
BstSFI CTRYAG 3 cut(s) 111, 387, 1341
BstUI CGCG 1 cut(s) 1148
BstV1I GCAGC 5 cut(s) 125, 448, 484, 1129, 1246
BstV2I GAAGAC 1 cut(s) 1082
BstZI CGGCCG 1 cut(s) 904
Bsu36I CCTNAGG 1 cut(s) 852
BsuRI GGCC 4 cut(s) 23, 465, 510, 906
BtgI CCRYGG 1 cut(s) 594
BtsCI GGATG 4 cut(s) 157, 357, 1027, 1318
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 2 cut(s) 208, 629
BveI ACCTGC 1 cut(s) 404
Cac8I GCNNGC 3 cut(s) 217, 747, 971
CaiI CAGNNNCTG 1 cut(s) 935
CfoI GCGC 2 cut(s) 338, 1348
Cfr13I GGNCC 5 cut(s) 80, 248, 416, 1013, 1175
Csp6I GTAC 1 cut(s) 627
CviAII CATG 2 cut(s) 62, 716
CviQI GTAC 1 cut(s) 627
DpnI GATC 1 cut(s) 454
DpnII GATC 1 cut(s) 452
DraI TTTAAA 1 cut(s) 808
EaeI YGGCCR 2 cut(s) 463, 904
EagI CGGCCG 1 cut(s) 904
Eam1104I CTCTTC 5 cut(s) 676, 908, 1059, 1091, 1302
EarI CTCTTC 5 cut(s) 676, 908, 1059, 1091, 1302
EciI GGCGGA 1 cut(s) 834
EclXI CGGCCG 1 cut(s) 904
Eco130I CCWWGG 2 cut(s) 617, 1326
Eco147I AGGCCT 1 cut(s) 510
Eco47I GGWCC 5 cut(s) 80, 248, 416, 1013, 1175
Eco47III AGCGCT 1 cut(s) 1347
Eco52I CGGCCG 1 cut(s) 904
Eco57I CTGAAG 3 cut(s) 20, 525, 957
Eco81I CCTNAGG 1 cut(s) 852
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoO109I RGGNCCY 1 cut(s) 1013
EcoRII CCWGG 1 cut(s) 1253
EcoT14I CCWWGG 2 cut(s) 617, 1326
ErhI CCWWGG 2 cut(s) 617, 1326
FaeI CATG 2 cut(s) 65, 719
FaiI YATR 8 cut(s) 30, 63, 168, 555, 608, 642, 717, 1343
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 4 cut(s) 261, 312, 576, 1264
FatI CATG 2 cut(s) 61, 715
Fnu4HI GCNGC 8 cut(s) 24, 114, 437, 473, 907, 1143, 1146, 1260
FokI GGATG 4 cut(s) 164, 364, 1014, 1305
Fsp4HI GCNGC 8 cut(s) 24, 114, 437, 473, 907, 1143, 1146, 1260
FspBI CTAG 4 cut(s) 324, 618, 767, 1059
GlaI GCGC 2 cut(s) 337, 1347
GluI GCNGC 8 cut(s) 24, 114, 437, 473, 907, 1143, 1146, 1260
GsuI CTGGAG 2 cut(s) 201, 909
HaeII RGCGCY 2 cut(s) 339, 1349
HaeIII GGCC 4 cut(s) 23, 465, 510, 906
HapII CCGG 1 cut(s) 1241
HhaI GCGC 2 cut(s) 338, 1348
Hin1II CATG 2 cut(s) 65, 719
Hin6I GCGC 2 cut(s) 336, 1346
HinP1I GCGC 2 cut(s) 336, 1346
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 826
HinfI GANTC 5 cut(s) 5, 197, 572, 932, 958
HpaII CCGG 1 cut(s) 1241
HphI GGTGA 4 cut(s) 94, 101, 154, 995
Hpy166II GTNNAC 3 cut(s) 145, 208, 629
Hpy188I TCNGA 7 cut(s) 10, 151, 351, 490, 937, 1072, 1288
Hpy188III TCNNGA 6 cut(s) 47, 83, 767, 790, 836, 1334
Hpy8I GTNNAC 3 cut(s) 145, 208, 629
HpyAV CCTTC 6 cut(s) 283, 500, 593, 932, 1026, 1102
HpyCH4III ACNGT 3 cut(s) 89, 947, 1300
HpyCH4V TGCA 9 cut(s) 113, 215, 286, 380, 719, 745, 1027, 1160, 1262
HpyF10VI GCNNNNNNNGC 2 cut(s) 915, 1345
Hsp92II CATG 2 cut(s) 65, 719
HspAI GCGC 2 cut(s) 336, 1346
Kzo9I GATC 1 cut(s) 452
LguI GCTCTTC 1 cut(s) 908
LmnI GCTCC 4 cut(s) 554, 838, 859, 1273
Lsp1109I GCAGC 5 cut(s) 125, 448, 484, 1129, 1246
LweI GCATC 3 cut(s) 367, 774, 1036
MaeI CTAG 4 cut(s) 324, 618, 767, 1059
MaeIII GTNAC 2 cut(s) 870, 1111
MalI GATC 1 cut(s) 454
MboI GATC 1 cut(s) 452
MlsI TGGCCA 1 cut(s) 465
MluCI AATT 8 cut(s) 13, 129, 169, 345, 421, 526, 739, 1354
MluNI TGGCCA 1 cut(s) 465
MlyI GAGTC 2 cut(s) 566, 967
Mox20I TGGCCA 1 cut(s) 465
MroXI GAANNNNTTC 1 cut(s) 1248
MscI TGGCCA 1 cut(s) 465
MseI TTAA 5 cut(s) 587, 654, 780, 807, 1302
MslI CAYNNNNRTG 1 cut(s) 1287
Msp20I TGGCCA 1 cut(s) 465
MspI CCGG 1 cut(s) 1241
MspR9I CCNGG 1 cut(s) 1255
MvaI CCWGG 1 cut(s) 1255
MvnI CGCG 1 cut(s) 1148
MwoI GCNNNNNNNGC 2 cut(s) 915, 1345
NdeII GATC 1 cut(s) 452
NlaIII CATG 2 cut(s) 65, 719
NlaIV GGNNCC 4 cut(s) 249, 495, 550, 840
NmeAIII GCCGAG 1 cut(s) 882
NspI RCATGY 1 cut(s) 719
PasI CCCWGGG 1 cut(s) 1254
PceI AGGCCT 1 cut(s) 510
PciSI GCTCTTC 1 cut(s) 908
PdmI GAANNNNTTC 1 cut(s) 1248
PfeI GAWTC 3 cut(s) 5, 197, 932
PflMI CCANNNNNTGG 1 cut(s) 641
PkrI GCNGC 8 cut(s) 25, 115, 438, 474, 908, 1144, 1147, 1261
PleI GAGTC 2 cut(s) 566, 966
PpsI GAGTC 2 cut(s) 566, 966
PpuMI RGGWCCY 1 cut(s) 1013
Psp5II RGGWCCY 1 cut(s) 1013
Psp6I CCWGG 1 cut(s) 1253
PspGI CCWGG 1 cut(s) 1253
PspN4I GGNNCC 4 cut(s) 249, 495, 550, 840
PspPI GGNCC 5 cut(s) 80, 248, 416, 1013, 1175
PspPPI RGGWCCY 1 cut(s) 1013
PstI CTGCAG 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 935
RsaI GTAC 1 cut(s) 628
RsaNI GTAC 1 cut(s) 627
RseI CAYNNNNRTG 1 cut(s) 1287
SapI GCTCTTC 1 cut(s) 908
SaqAI TTAA 5 cut(s) 587, 654, 780, 807, 1302
SatI GCNGC 8 cut(s) 24, 114, 437, 473, 907, 1143, 1146, 1260
Sau3AI GATC 1 cut(s) 452
Sau96I GGNCC 5 cut(s) 80, 248, 416, 1013, 1175
SchI GAGTC 2 cut(s) 566, 967
ScrFI CCNGG 1 cut(s) 1255
SfaNI GCATC 3 cut(s) 367, 774, 1036
SfcI CTRYAG 3 cut(s) 111, 387, 1341
SinI GGWCC 5 cut(s) 80, 248, 416, 1013, 1175
SmiMI CAYNNNNRTG 1 cut(s) 1287
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
Sse9I AATT 8 cut(s) 13, 129, 169, 345, 421, 526, 739, 1354
SseBI AGGCCT 1 cut(s) 510
SsiI CCGC 5 cut(s) 24, 845, 907, 1105, 1146
SspMI CTAG 4 cut(s) 324, 618, 767, 1059
StuI AGGCCT 1 cut(s) 510
StyD4I CCNGG 1 cut(s) 1253
StyI CCWWGG 2 cut(s) 617, 1326
TaaI ACNGT 3 cut(s) 89, 947, 1300
TaqI TCGA 1 cut(s) 500
TaqII GACCGA 1 cut(s) 265
TasI AATT 8 cut(s) 13, 129, 169, 345, 421, 526, 739, 1354
TauI GCSGC 3 cut(s) 26, 909, 1148
TfiI GAWTC 3 cut(s) 5, 197, 932
Tru1I TTAA 5 cut(s) 587, 654, 780, 807, 1302
Tru9I TTAA 5 cut(s) 587, 654, 780, 807, 1302
TscAI CASTG 2 cut(s) 208, 636
TseI GCWGC 5 cut(s) 113, 436, 472, 1142, 1259
TspDTI ATGAA 5 cut(s) 162, 368, 623, 678, 1320
TspGWI ACGGA 1 cut(s) 611
TspRI CASTG 2 cut(s) 208, 636
Van91I CCANNNNNTGG 1 cut(s) 641
VpaK11BI GGWCC 5 cut(s) 80, 248, 416, 1013, 1175
XagI CCTNNNNNAGG 1 cut(s) 515
XapI RAATTY 2 cut(s) 421, 526
XbaI TCTAGA 1 cut(s) 766
XceI RCATGY 1 cut(s) 719
XmaJI CCTAGG 1 cut(s) 617
XmnI GAANNNNTTC 1 cut(s) 1248
XspI CTAG 4 cut(s) 324, 618, 767, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.