Rh6BG514300

kDa class II heat shock protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001449
Physical Location & Seq
Reverse (-)
70894520 .. 70895724
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG514300.1

Sequence Viewer

Length: 624 bp
ATGGACCTCATGATTCCCCTGAGGAATATGGGTGTCGACACCAACCTCCTGGACGCCCTCCACGACCTTTTGGACTTCTCTGACGAGCAAAATCAGGCGAGCCACCATGCGCCTTCGCGCCAATACGTCCGGGAAGCCAAGGCCATGTCGGCTACTCCAGCTGACGTGAAGGAGACTCAGAATGCCTACATCTTTGTGATAGATGTGCCCGGGCTGAAGTCGGACCAGATCAAAGTCCATCTGGAAGACGACAACGTTTTGGTGGTGTGCGGCGAGAGAAAGAGGGAGAAGGAGAGAGACCAGGGAGTGAGGTACCTGAGGATGGAGAGGAGGCTCGGCAAGTATCTCAAGAAGTTTGTGCTGCCGGAGAATGCTGATGTAGAGAAGATCAGTGCCGAGTGTCAGGATGGGGTGTTGACTATTGTGGTGGAGAAGAAGCCGCCGCCGGAGCTGAAGAAGCCCAAGATTGTTCAGGTGAGGATTGGCGACAGCCAAGAAGGCCAACAGGGTCAAGGTGGCCAGTGGGGCCAAGAGGGTCAAGGTGGAGGTCAAGGAGACCAGTGGGGTCAAAAGGGCCAAGGAGGTCAAGAGGGTCAGGGTGGCCAAGATGGCAACAGCCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

207

Amino Acids

22.97

Weight (kDa)

5.48

Isoelectric Point (pI)

31.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 55 - 159 1e-26 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 1 cut(s) 312
AccI GTMKAC 1 cut(s) 36
AccII CGCG 1 cut(s) 118
AciI CCGC 4 cut(s) 270, 440, 443, 619
AclI AACGTT 1 cut(s) 255
AcoI YGGCCR 2 cut(s) 517, 601
AcuI CTGAAG 2 cut(s) 236, 473
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 322
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 2 cut(s) 48, 300
AluBI AGCT 2 cut(s) 161, 451
AluI AGCT 2 cut(s) 161, 451
Alw26I GTCTC 3 cut(s) 167, 291, 549
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 6 cut(s) 141, 499, 517, 526, 574, 601
ApeKI GCWGC 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 312
AspLEI GCGC 2 cut(s) 112, 120
AspS9I GGNCC 4 cut(s) 4, 223, 526, 574
AsuC2I CCSGG 3 cut(s) 131, 210, 211
AsuHPI GGTGA 1 cut(s) 487
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 2 cut(s) 4, 223
AxyI CCTNAGG 2 cut(s) 20, 317
BaeGI GKGCMC 1 cut(s) 210
BalI TGGCCA 2 cut(s) 519, 603
BanI GGYRCC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 348
BccI CCATC 4 cut(s) 246, 316, 401, 602
BciT130I CCWGG 2 cut(s) 50, 302
BcnI CCSGG 3 cut(s) 131, 210, 211
BcoDI GTCTC 3 cut(s) 167, 291, 549
BfaI CTAG 1 cut(s) 622
BglI GCCNNNNNGGC 4 cut(s) 149, 498, 525, 609
BisI GCNGC 5 cut(s) 271, 362, 440, 443, 619
BlsI GCNGC 5 cut(s) 272, 363, 441, 444, 620
Bme1390I CCNGG 5 cut(s) 50, 131, 210, 211, 302
Bme18I GGWCC 2 cut(s) 4, 223
BmeT110I CYCGRG 1 cut(s) 209
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 4 cut(s) 4, 223, 526, 574
BmiI GGNNCC 2 cut(s) 314, 527
BmrFI CCNGG 5 cut(s) 50, 131, 210, 211, 302
BpiI GAAGAC 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 3 cut(s) 131, 210, 211
BsaHI GRCGYC 1 cut(s) 54
BsaI GGTCTC 2 cut(s) 291, 549
BsaJI CCNNGG 4 cut(s) 138, 209, 301, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse1I ACTGG 2 cut(s) 520, 559
Bse21I CCTNAGG 2 cut(s) 20, 317
BseBI CCWGG 2 cut(s) 50, 302
BseDI CCNNGG 4 cut(s) 138, 209, 301, 577
BseGI GGATG 2 cut(s) 327, 412
BseLI CCNNNNNNNGG 1 cut(s) 322
BseMII CTCAG 3 cut(s) 11, 191, 308
BseNI ACTGG 2 cut(s) 520, 559
BseRI GAGGAG 1 cut(s) 343
BseSI GKGCMC 1 cut(s) 210
BseXI GCAGC 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 6 cut(s) 143, 501, 519, 528, 576, 603
BshNI GGYRCC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 130, 210, 365, 446
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 3 cut(s) 167, 291, 549
BsmI GAATGC 2 cut(s) 187, 376
BsnI GGCC 6 cut(s) 143, 501, 519, 528, 576, 603
Bso31I GGTCTC 2 cut(s) 291, 549
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 2 cut(s) 228, 387
BspACI CCGC 4 cut(s) 270, 440, 443, 619
BspANI GGCC 6 cut(s) 143, 501, 519, 528, 576, 603
BspCNI CTCAG 3 cut(s) 12, 190, 309
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 2 cut(s) 314, 527
BspT107I GGYRCC 1 cut(s) 312
BspTNI GGTCTC 2 cut(s) 291, 549
BsrI ACTGG 2 cut(s) 520, 559
BssECI CCNNGG 4 cut(s) 138, 209, 301, 577
BssMI GATC 2 cut(s) 228, 387
BssNI GRCGYC 1 cut(s) 54
BssT1I CCWWGG 2 cut(s) 138, 577
Bst2UI CCWGG 2 cut(s) 50, 302
BstACI GRCGYC 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 3 cut(s) 20, 177, 317
BstF5I GGATG 2 cut(s) 327, 412
BstFNI CGCG 1 cut(s) 118
BstHHI GCGC 2 cut(s) 112, 120
BstKTI GATC 2 cut(s) 231, 390
BstMAI GTCTC 3 cut(s) 167, 291, 549
BstMBI GATC 2 cut(s) 228, 387
BstMWI GCNNNNNNNGC 8 cut(s) 149, 158, 448, 457, 498, 525, 609, 618
BstNI CCWGG 2 cut(s) 50, 302
BstSCI CCNGG 5 cut(s) 48, 129, 208, 209, 300
BstSLI GKGCMC 1 cut(s) 210
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 348
BstV2I GAAGAC 1 cut(s) 252
BstXI CCANNNNNNTGG 1 cut(s) 49
Bsu36I CCTNAGG 2 cut(s) 20, 317
BsuRI GGCC 6 cut(s) 143, 501, 519, 528, 576, 603
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 2 cut(s) 327, 412
BtsIMutI CAGTG 3 cut(s) 397, 527, 566
Cac8I GCNNGC 1 cut(s) 100
CciI TCATGA 1 cut(s) 9
CfoI GCGC 2 cut(s) 112, 120
Cfr13I GGNCC 4 cut(s) 4, 223, 526, 574
Cfr9I CCCGGG 1 cut(s) 209
CseI GACGC 1 cut(s) 62
Csp6I GTAC 1 cut(s) 313
CviAII CATG 3 cut(s) 10, 107, 145
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 3 cut(s) 20, 177, 317
DpnI GATC 2 cut(s) 230, 389
DpnII GATC 2 cut(s) 228, 387
EaeI YGGCCR 2 cut(s) 517, 601
Eco130I CCWWGG 2 cut(s) 138, 577
Eco31I GGTCTC 2 cut(s) 291, 549
Eco47I GGWCC 2 cut(s) 4, 223
Eco57I CTGAAG 2 cut(s) 236, 473
Eco81I CCTNAGG 2 cut(s) 20, 317
Eco88I CYCGRG 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 48, 300
EcoT14I CCWWGG 2 cut(s) 138, 577
ErhI CCWWGG 2 cut(s) 138, 577
FaeI CATG 3 cut(s) 13, 110, 148
FaiI YATR 4 cut(s) 11, 29, 108, 146
FatI CATG 3 cut(s) 9, 106, 144
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 5 cut(s) 271, 362, 440, 443, 619
FokI GGATG 2 cut(s) 334, 419
Fsp4HI GCNGC 5 cut(s) 271, 362, 440, 443, 619
FspBI CTAG 1 cut(s) 622
GlaI GCGC 2 cut(s) 111, 119
GluI GCNGC 5 cut(s) 271, 362, 440, 443, 619
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 6 cut(s) 143, 501, 519, 528, 576, 603
HapII CCGG 4 cut(s) 130, 210, 365, 446
HgaI GACGC 1 cut(s) 62
HhaI GCGC 2 cut(s) 112, 120
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 3 cut(s) 13, 110, 148
Hin6I GCGC 2 cut(s) 110, 118
HinP1I GCGC 2 cut(s) 110, 118
HincII GTYRAC 2 cut(s) 37, 417
HindII GTYRAC 2 cut(s) 37, 417
HinfI GANTC 2 cut(s) 13, 175
HpaII CCGG 4 cut(s) 130, 210, 365, 446
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 2 cut(s) 37, 417
Hpy188I TCNGA 3 cut(s) 82, 180, 223
Hpy188III TCNNGA 5 cut(s) 10, 242, 349, 404, 587
Hpy8I GTNNAC 2 cut(s) 37, 417
HpyAV CCTTC 4 cut(s) 123, 163, 283, 491
HpyCH4IV ACGT 3 cut(s) 126, 165, 255
HpyF10VI GCNNNNNNNGC 8 cut(s) 149, 158, 448, 457, 498, 525, 609, 618
HpyF3I CTNAG 3 cut(s) 20, 177, 317
HpySE526I ACGT 3 cut(s) 126, 165, 255
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 3 cut(s) 13, 110, 148
HspAI GCGC 2 cut(s) 110, 118
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 2 cut(s) 228, 387
LmnI GCTCC 1 cut(s) 448
Lsp1109I GCAGC 1 cut(s) 348
MaeI CTAG 1 cut(s) 622
MaeII ACGT 3 cut(s) 126, 165, 255
MalI GATC 2 cut(s) 230, 389
MboI GATC 2 cut(s) 228, 387
MboII GAAGA 4 cut(s) 257, 397, 445, 466
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 2 cut(s) 519, 603
MluNI TGGCCA 2 cut(s) 519, 603
MlyI GAGTC 1 cut(s) 169
MmeI TCCRAC 1 cut(s) 201
Mox20I TGGCCA 2 cut(s) 519, 603
MscI TGGCCA 2 cut(s) 519, 603
MslI CAYNNNNRTG 1 cut(s) 194
Msp20I TGGCCA 2 cut(s) 519, 603
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 4 cut(s) 130, 210, 365, 446
MspR9I CCNGG 5 cut(s) 50, 131, 210, 211, 302
Mva1269I GAATGC 2 cut(s) 187, 376
MvaI CCWGG 2 cut(s) 50, 302
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 8 cut(s) 149, 158, 448, 457, 498, 525, 609, 618
NciI CCSGG 3 cut(s) 131, 210, 211
NdeII GATC 2 cut(s) 228, 387
NlaIII CATG 3 cut(s) 13, 110, 148
NlaIV GGNNCC 2 cut(s) 314, 527
NmeAIII GCCGAG 2 cut(s) 315, 421
PagI TCATGA 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 2 cut(s) 187, 376
PfeI GAWTC 1 cut(s) 13
PfoI TCCNGGA 2 cut(s) 48, 129
PkrI GCNGC 5 cut(s) 272, 363, 441, 444, 620
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
Psp1406I AACGTT 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 48, 300
PspGI CCWGG 2 cut(s) 48, 300
PspN4I GGNNCC 2 cut(s) 314, 527
PspPI GGNCC 4 cut(s) 4, 223, 526, 574
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 1 cut(s) 194
SalI GTCGAC 1 cut(s) 35
SatI GCNGC 5 cut(s) 271, 362, 440, 443, 619
Sau3AI GATC 2 cut(s) 228, 387
Sau96I GGNCC 4 cut(s) 4, 223, 526, 574
SchI GAGTC 1 cut(s) 169
ScrFI CCNGG 5 cut(s) 50, 131, 210, 211, 302
SduI GDGCHC 1 cut(s) 210
SfiI GGCCNNNNNGGCC 1 cut(s) 525
SinI GGWCC 2 cut(s) 4, 223
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 194
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
SrfI GCCCGGGC 1 cut(s) 211
SsiI CCGC 4 cut(s) 270, 440, 443, 619
SspMI CTAG 1 cut(s) 622
StyD4I CCNGG 5 cut(s) 48, 129, 208, 209, 300
StyI CCWWGG 2 cut(s) 138, 577
TaiI ACGT 3 cut(s) 129, 168, 258
TaqI TCGA 1 cut(s) 36
TauI GCSGC 4 cut(s) 273, 442, 445, 621
TfiI GAWTC 1 cut(s) 13
TscAI CASTG 3 cut(s) 397, 527, 566
TseI GCWGC 1 cut(s) 361
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 3 cut(s) 397, 527, 566
VpaK11BI GGWCC 2 cut(s) 4, 223
XmaI CCCGGG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 36
XspI CTAG 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.