Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001449

Physical Location & Seq

Genomic Coordinates Reverse (-)

5474013 .. 5474495

Gene Structure 483 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1068800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 483 bp

ATGGATTTGATAAACGGGGGAAACAGTTTCTTCAGAGTATTGGAAGACATGCTGGACTTCGCCGATCAAGAGCCCGAAAAGTCCCGAAACGCCAACAACCCCTCTCGGGCGTACGTCCGAGACGCGAAGGCGATGGCGGCCACCCCAGCGGATGTGGTGGAGTACCCGAACGCGTACGTGTTCGTGGTGGACATGCCTGGAATCAAAGCCAATGAAATTAAAGTGCAGGTGGAGAACGACAACGTGTTGGTGGTGAGCGGGGAGCGGAGGGGGGAGAAAGAGAAGGAGAGCAAGGATGCGGTGAAGTATTTGAAGATGGAAAGGAGGGTTGGGAAGTTTATGAGGAAGTTTGTGCTGCCGGAGAATGCGAACTTGGAGGCGATCACGGCGGTTTCTCAAGACGGCGTGCTGACGGTGAGGGTGGAGAAGTTGCCTCCGCCAGAGCCCAAGAGGGCCAAGACCATTCAGGTCAGTGTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

HSP20

Protein Analysis

161

Amino Acids

17.9

Weight (kDa)

6.41

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP20	PF00011	52 - 158	1.7e-27	Hsp20/alpha crystallin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0001449)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12020 AT5G12030
fragaria_vesca	FvH4_2g31371
malus_domestica	MD00G1181700.v1.1 MD08G1068000.v1.1 MD08G1068200.v1.1 MD08G1068300.v1.1 MD08G1068500.v1.1 MD08G1068700.v1.1 MD08G1068800.v1.1 MD15G1053500.v1.1 MD15G1053600.v1.1 MD15G1053800.v1.1
prunus_persica	Prupe.1G407100_v2.0.a1 Prupe.1G407200_v2.0.a1 Prupe.1G407300_v2.0.a1
pyrus_communis	pycom08g05660 pycom08g05670
rosa_chinensis	RchiOBHm_Chr6g0311451 RchiOBHm_Chr6g0311461 RchiOBHm_Chr6g0311471
rosa_laevigata	RLG00000010403 RLG00000010404 RLG00000010405
rosa_multiflora	Rmu_co8421495.1_g000001 Rmu_sc0006915.1_g000010 Rmu_sc0006915.1_g000011 Rmu_sc0006915.1_g000012 Rmu_sc0013619.1_g000003 Rmu_sc0013619.1_g000004 Rmu_sc0013619.1_g000006 Rmu_sc0015790.1_g000003 Rmu_sc0015790.1_g000004
rosa_roxburghii	Rroxscaffold_7G00157590 Rroxscaffold_7G00157600
rosa_rugosa	Rorug06G0389100 Rorug06G0389100 Rorug06G0389200
rosa_samantha	Rh2AG205100 Rh2BG216900 Rh2DG211400 Rh6AG503500 Rh6AG503700 Rh6BG514200 Rh6BG514300 Rh6CG520000 Rh6CG520100 Rh6DG506100
rosa_wichuraiana	Rw2G016340 Rw6G043850 Rw6G043860 Rw6G043870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	217
Acc36I	ACCTGC	1 cut(s)	217
AccBSI	CCGCTC	2 cut(s)	258, 265
AccII	CGCG	2 cut(s)	125, 173
AciI	CCGC	7 cut(s)	137, 149, 258, 265, 299, 389, 437
AcoI	YGGCCR	1 cut(s)	138
AcuI	CTGAAG	1 cut(s)	16
AfaI	GTAC	3 cut(s)	113, 164, 176
AfiI	CCNNNNNNNGG	1 cut(s)	106
AflIII	ACRYGT	3 cut(s)	171, 177, 243
AgsI	TTSAA	1 cut(s)	313
AjnI	CCWGG	1 cut(s)	196
AjuI	GAANNNNNNNTTGG	4 cut(s)	312, 344, 356, 388
Alw26I	GTCTC	1 cut(s)	114
Ama87I	CYCGRG	1 cut(s)	105
AoxI	GGCC	2 cut(s)	138, 453
ApeKI	GCWGC	1 cut(s)	355
AspS9I	GGNCC	1 cut(s)	453
AsuHPI	GGTGA	3 cut(s)	265, 313, 427
AvaI	CYCGRG	1 cut(s)	105
BanII	GRGCYC	2 cut(s)	75, 447
BbsI	GAAGAC	1 cut(s)	51
BbvI	GCAGC	1 cut(s)	342
BccI	CCATC	2 cut(s)	127, 310
BceAI	ACGGC	2 cut(s)	402, 418
BciT130I	CCWGG	1 cut(s)	198
BcoDI	GTCTC	1 cut(s)	114
BfuAI	ACCTGC	1 cut(s)	217
BisI	GCNGC	2 cut(s)	138, 356
BlsI	GCNGC	2 cut(s)	139, 357
Bme1390I	CCNGG	1 cut(s)	198
BmeT110I	CYCGRG	1 cut(s)	105
BmgT120I	GGNCC	1 cut(s)	453
BmrFI	CCNGG	1 cut(s)	198
BmsI	GCATC	1 cut(s)	286
BpiI	GAAGAC	1 cut(s)	51
BpuEI	CTTGAG	1 cut(s)	381
BsaAI	YACGTR	1 cut(s)	178
Bsc4I	CCNNNNNNNGG	1 cut(s)	106
BseBI	CCWGG	1 cut(s)	198
BseGI	GGATG	2 cut(s)	157, 301
BseLI	CCNNNNNNNGG	1 cut(s)	106
BseXI	GCAGC	1 cut(s)	342
BseYI	CCCAGC	1 cut(s)	145
BsgI	GTGCAG	1 cut(s)	245
Bsh1236I	CGCG	2 cut(s)	125, 173
BshFI	GGCC	2 cut(s)	140, 455
BsiHKCI	CYCGRG	1 cut(s)	105
BsiSI	CCGG	1 cut(s)	359
BsiWI	CGTACG	2 cut(s)	111, 174
BslFI	GGGAC	1 cut(s)	67
BslI	CCNNNNNNNGG	1 cut(s)	106
BsmAI	GTCTC	1 cut(s)	114
BsmBI	CGTCTC	1 cut(s)	114
BsmFI	GGGAC	1 cut(s)	67
BsmI	GAATGC	1 cut(s)	370
BsnI	GGCC	2 cut(s)	140, 455
BsoBI	CYCGRG	1 cut(s)	105
Bsp1286I	GDGCHC	2 cut(s)	75, 447
Bsp143I	GATC	2 cut(s)	64, 381
BspACI	CCGC	7 cut(s)	137, 149, 258, 265, 299, 389, 437
BspANI	GGCC	2 cut(s)	140, 455
BspFNI	CGCG	2 cut(s)	125, 173
BspMI	ACCTGC	1 cut(s)	217
BsrBI	CCGCTC	2 cut(s)	258, 265
BssMI	GATC	2 cut(s)	64, 381
Bst2UI	CCWGG	1 cut(s)	198
Bst4CI	ACNGT	2 cut(s)	26, 415
BstBAI	YACGTR	1 cut(s)	178
BstC8I	GCNNGC	1 cut(s)	407
BstF5I	GGATG	2 cut(s)	157, 301
BstFNI	CGCG	2 cut(s)	125, 173
BstKTI	GATC	2 cut(s)	67, 384
BstMAI	GTCTC	1 cut(s)	114
BstMBI	GATC	2 cut(s)	64, 381
BstMWI	GCNNNNNNNGC	3 cut(s)	137, 146, 386
BstNI	CCWGG	1 cut(s)	198
BstNSI	RCATGY	2 cut(s)	52, 196
BstSCI	CCNGG	1 cut(s)	196
BstUI	CGCG	2 cut(s)	125, 173
BstV1I	GCAGC	1 cut(s)	342
BstV2I	GAAGAC	1 cut(s)	51
BsuRI	GGCC	2 cut(s)	140, 455
BtgZI	GCGATG	1 cut(s)	146
BtsCI	GGATG	2 cut(s)	157, 301
BtsIMutI	CAGTG	1 cut(s)	478
BveI	ACCTGC	1 cut(s)	217
Cac8I	GCNNGC	1 cut(s)	407
Cfr13I	GGNCC	1 cut(s)	453
CseI	GACGC	1 cut(s)	131
Csp6I	GTAC	3 cut(s)	112, 163, 175
CviAII	CATG	2 cut(s)	49, 193
CviJI	RGCY	5 cut(s)	73, 140, 209, 445, 455
CviKI_1	RGCY	5 cut(s)	73, 140, 209, 445, 455
CviQI	GTAC	3 cut(s)	112, 163, 175
DpnI	GATC	2 cut(s)	66, 383
DpnII	GATC	2 cut(s)	64, 381
EaeI	YGGCCR	1 cut(s)	138
EciI	GGCGGA	1 cut(s)	426
Eco24I	GRGCYC	2 cut(s)	75, 447
Eco57I	CTGAAG	1 cut(s)	16
Eco88I	CYCGRG	1 cut(s)	105
EcoRII	CCWGG	1 cut(s)	196
EcoT38I	GRGCYC	2 cut(s)	75, 447
Esp3I	CGTCTC	1 cut(s)	114
FaeI	CATG	2 cut(s)	52, 196
FaiI	YATR	3 cut(s)	50, 194, 341
FaqI	GGGAC	1 cut(s)	67
FatI	CATG	2 cut(s)	48, 192
FauI	CCCGC	1 cut(s)	251
Fnu4HI	GCNGC	2 cut(s)	138, 356
FokI	GGATG	2 cut(s)	164, 308
FriOI	GRGCYC	2 cut(s)	75, 447
Fsp4HI	GCNGC	2 cut(s)	138, 356
GluI	GCNGC	2 cut(s)	138, 356
GsaI	CCCAGC	1 cut(s)	149
HaeIII	GGCC	2 cut(s)	140, 455
HapII	CCGG	1 cut(s)	359
HgaI	GACGC	1 cut(s)	131
Hin1II	CATG	2 cut(s)	52, 196
HinfI	GANTC	1 cut(s)	201
HpaII	CCGG	1 cut(s)	359
HphI	GGTGA	3 cut(s)	265, 313, 427
Hpy166II	GTNNAC	1 cut(s)	190
Hpy188I	TCNGA	2 cut(s)	35, 119
Hpy188III	TCNNGA	3 cut(s)	68, 84, 398
Hpy8I	GTNNAC	1 cut(s)	190
HpyAV	CCTTC	2 cut(s)	121, 277
HpyCH4III	ACNGT	2 cut(s)	26, 415
HpyCH4IV	ACGT	3 cut(s)	114, 177, 243
HpyCH4V	TGCA	1 cut(s)	226
HpyF10VI	GCNNNNNNNGC	3 cut(s)	137, 146, 386
HpySE526I	ACGT	3 cut(s)	114, 177, 243
Hsp92II	CATG	2 cut(s)	52, 196
Kzo9I	GATC	2 cut(s)	64, 381
LmnI	GCTCC	1 cut(s)	262
LpnPI	CCDG	8 cut(s)	38, 159, 183, 210, 212, 372, 452, 453
Lsp1109I	GCAGC	1 cut(s)	342
LweI	GCATC	1 cut(s)	286
MaeII	ACGT	3 cut(s)	114, 177, 243
MalI	GATC	2 cut(s)	66, 383
MbiI	CCGCTC	2 cut(s)	258, 265
MboI	GATC	2 cut(s)	64, 381
MboII	GAAGA	3 cut(s)	22, 56, 325
MhlI	GDGCHC	2 cut(s)	75, 447
MluCI	AATT	1 cut(s)	216
MluI	ACGCGT	1 cut(s)	171
MnlI	CCTC	8 cut(s)	112, 261, 318, 336, 370, 411, 444, 444
MseI	TTAA	1 cut(s)	219
MspA1I	CMGCKG	1 cut(s)	149
MspI	CCGG	1 cut(s)	359
MspR9I	CCNGG	1 cut(s)	198
Mva1269I	GAATGC	1 cut(s)	370
MvaI	CCWGG	1 cut(s)	198
MvnI	CGCG	2 cut(s)	125, 173
MwoI	GCNNNNNNNGC	3 cut(s)	137, 146, 386
NdeII	GATC	2 cut(s)	64, 381
NlaIII	CATG	2 cut(s)	52, 196
NspI	RCATGY	2 cut(s)	52, 196
PaqCI	CACCTGC	1 cut(s)	217
PctI	GAATGC	1 cut(s)	370
PfeI	GAWTC	1 cut(s)	201
Pfl23II	CGTACG	2 cut(s)	111, 174
PkrI	GCNGC	2 cut(s)	139, 357
Ppu21I	YACGTR	1 cut(s)	178
Psp6I	CCWGG	1 cut(s)	196
PspFI	CCCAGC	1 cut(s)	145
PspGI	CCWGG	1 cut(s)	196
PspLI	CGTACG	2 cut(s)	111, 174
PspPI	GGNCC	1 cut(s)	453
RsaI	GTAC	3 cut(s)	113, 164, 176
RsaNI	GTAC	3 cut(s)	112, 163, 175
SaqAI	TTAA	1 cut(s)	219
SatI	GCNGC	2 cut(s)	138, 356
Sau3AI	GATC	2 cut(s)	64, 381
Sau96I	GGNCC	1 cut(s)	453
ScrFI	CCNGG	1 cut(s)	198
SduI	GDGCHC	2 cut(s)	75, 447
SetI	ASST	5 cut(s)	117, 180, 231, 246, 471
SfaNI	GCATC	1 cut(s)	286
SmlI	CTYRAG	1 cut(s)	396
SmoI	CTYRAG	1 cut(s)	396
Sse9I	AATT	1 cut(s)	216
SsiI	CCGC	7 cut(s)	137, 149, 258, 265, 299, 389, 437
StyD4I	CCNGG	1 cut(s)	196
TaaI	ACNGT	2 cut(s)	26, 415
TaiI	ACGT	3 cut(s)	117, 180, 246
TasI	AATT	1 cut(s)	216
TauI	GCSGC	1 cut(s)	140
TfiI	GAWTC	1 cut(s)	201
Tru1I	TTAA	1 cut(s)	219
Tru9I	TTAA	1 cut(s)	219
TscAI	CASTG	1 cut(s)	478
TseI	GCWGC	1 cut(s)	355
TspDTI	ATGAA	1 cut(s)	228
TspRI	CASTG	1 cut(s)	478
XceI	RCATGY	2 cut(s)	52, 196

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.